CN105265159B - Knoxia valerianoide tissue-cultured seedling cultural method - Google Patents
Knoxia valerianoide tissue-cultured seedling cultural method Download PDFInfo
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- CN105265159B CN105265159B CN201510810887.5A CN201510810887A CN105265159B CN 105265159 B CN105265159 B CN 105265159B CN 201510810887 A CN201510810887 A CN 201510810887A CN 105265159 B CN105265159 B CN 105265159B
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- 244000188405 Knoxia valerianoides Species 0.000 title claims abstract description 106
- 238000000034 method Methods 0.000 title claims abstract description 57
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 239000011159 matrix material Substances 0.000 claims abstract description 21
- 239000010815 organic waste Substances 0.000 claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract 2
- 230000004151 fermentation Effects 0.000 claims abstract 2
- 210000003608 fece Anatomy 0.000 claims description 38
- 239000010871 livestock manure Substances 0.000 claims description 33
- 239000012188 paraffin wax Substances 0.000 claims description 32
- 235000015097 nutrients Nutrition 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 244000025254 Cannabis sativa Species 0.000 claims description 15
- 239000010902 straw Substances 0.000 claims description 15
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 10
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 10
- 239000004563 wettable powder Substances 0.000 claims description 10
- 239000007921 spray Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 239000003337 fertilizer Substances 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- 241000287828 Gallus gallus Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 5
- KMQAPZBMEMMKSS-UHFFFAOYSA-K calcium;magnesium;phosphate Chemical compound [Mg+2].[Ca+2].[O-]P([O-])([O-])=O KMQAPZBMEMMKSS-UHFFFAOYSA-K 0.000 claims description 5
- 230000006378 damage Effects 0.000 claims description 5
- 239000003415 peat Substances 0.000 claims description 5
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 5
- 235000011009 potassium phosphates Nutrition 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000006041 probiotic Substances 0.000 claims description 5
- 235000018291 probiotics Nutrition 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 239000013535 sea water Substances 0.000 claims description 5
- 230000000529 probiotic effect Effects 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 19
- 241000196324 Embryophyta Species 0.000 abstract description 16
- 239000004576 sand Substances 0.000 abstract description 5
- 241000209094 Oryza Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
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- 239000007787 solid Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 235000013406 prebiotics Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 241000221079 Euphorbia <genus> Species 0.000 description 2
- 206010026749 Mania Diseases 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 1
- 235000018893 Cercis canadensis var canadensis Nutrition 0.000 description 1
- 240000000024 Cercis siliquastrum Species 0.000 description 1
- 241000434018 Euphorbia pekinensis Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000028446 budding cell bud growth Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 238000009400 out breeding Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a kind of Knoxia valerianoide tissue-cultured seedling cultural method, comprise the following steps：1) plant planting-line to prepare, in furrow face, laying depth is 5~10cm mixed planting matrix successively from down to up, and the thin river sand that thickness is 3~5cm forms kind of planting-line；Wherein, the mixed planting matrix is made by following steps：It is rotten that the well mixed complete fermentation heap that carries out of A liquid is sprayed by organic waste, then is sprayed B liquid and be sufficiently mixed and mix thoroughly；2) tissue-cultured seedling is transplanted；Compared with prior art, the Knoxia valerianoide tissue-cultured seedling transplanting survival rate and last survival rate of plant obtained using cultural method of the present invention efficiently solves the low bottleneck problem of the artificial cultivation scale survival rate of Knoxia valerianoide tissue-cultured seedling more than 95%.
Description
Technical field
The present invention relates to a kind of Knoxia valerianoide tissue-cultured seedling cultural method.It is more particularly related to a kind of significantly carry
The Knoxia valerianoide tissue-cultured seedling cultural method of high viability.
Background technology
Knoxia valerianoide (Knoxia valerianoides Thorel.) is Rubiaceae radix knoxiae category perennial herb
Medicinal plant, it is distributed in south China provinces and regions, alias radix knoxiae, the southern root of Beijing euphorbia, the purple root of Beijing euphorbia, vast halberd.
Knoxia valerianoide medicinal effects are root, there is diuresis, detumescence, dissipating bind and other effects, control chest and abdomen ponding, difficulty in urination and defecation, carbuncle swells sore
The diseases such as malicious, cold sick subcutaneous nodule (Fu Bing etc., the ecosystem characterization research of Medical Plant Knoxia Valerianoides), reuse can also treat manic psychosis (Yu Hui
The people, reuse radix knoxiae treatment manic psychosis 12)；The main ingredient of Chinese patent drug zijin troche is not only, and Guilin and medicine company share have
The main component of the main product " day and rheumatalgia-relieving plaster " of limit company production.
For many years, required medicinal material relies on wild resource acquisition, however, vegetation degeneration and people due to Knoxia valerianoide Proterozoic
Work indiscriminate mining and serious waste, wild resource quantity are drastically reduced, relatively low plus wild resource quantity setting percentage and seed germination rate, growth speed
Degree is slow, and natural crude drugs are far from meeting the needs of people, and at present, price has risen arrives 300 yuan of per kilogram.
Pan Jichang was early in 1989, i.e., by seed and root division progress introducing and planting, (Pan Jichang, radix knoxiae are introduced a fine variety
Cultivation technique)；After 1993, many researchers are carried out breeding to Knoxia valerianoide using biological technique method and obtain quantum jump
(Huang Hao, the research of Medical Plant Knoxia Valerianoides tissue cultures；Ling Zhengzhu etc., Knoxia valerianoide tissue cultures obtain regeneration plant；Chen Fang
It is clear etc., the tissue cultures of Medical Plant Knoxia Valerianoides；Qiu Anjing etc., the tissue cultures of radix knoxiae), but in the transplanting of tissue-cultured seedling
It is still to limit a bottleneck of artificial growth, transplanting survival rate highest only up to 88.36% (Huang Hao etc., red bud with cultivation survival rate
The research of Seedling in Euphorbia pekinensis transplantation technique).
At present, Knoxia valerianoide tissue-cultured seedling is similar to other most of tissue-cultured seedling, is first transplanted to the loose base of the water conservation containing ventilation
Cultivated in matter, after tissue-cultured seedling adapts to ambient atmosphere environment completely, then be transplanted to field planting；This kind repeatedly transplanted
Plant mode, grown for the stronger medicinal plant of root, bud growth ability, influence it is simultaneously little, but for grow it is weak, in migration process
In the Knoxia valerianoide tissue-cultured seedling that is easily damaged to hindering root and bud, have a great influence, virtually reduce the survival rate of tissue-cultured seedling.
Further, since Knoxia valerianoide is perennial medicinal plant, and every winter, the whole seedlings of aerial part, in order to ensure production
Amount, usually need to dig out potato root in the winter time during artificial growth, in room or in kiln, be embedded in ventilation, moistening sand or wood chip in vacation
Plant, plant potato root again to next year, planting tube shield workload is larger.
Therefore, the Knoxia valerianoide tissue-cultured seedling cultivation technique economical and practical, survival rate is high has turned into protection Knoxia valerianoide wild resource
The important topic urgently to be resolved hurrily with people's medication demand is met.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of Knoxia valerianoide tissue-cultured seedling cultural method, it is by tissue-cultured seedling in tissue culture bottle
Practice after seedling, the milpa being directly transplanted to, and no longer transplant or dig out again and plant again, not only reduce human and material resources
Expenditure, also improves plantation survival rate.
In order to realize according to object of the present invention and further advantage, there is provided a kind of Knoxia valerianoide tissue-cultured seedling cultivation side
Method, comprise the following steps：
1) planting-line is planted to prepare
In furrow face, laying depth is 5~10cm mixed planting matrix successively, and thickness is 3~5cm's from down to up
Thin river sand forms kind of a planting-line；
Wherein, the mixed planting matrix is made by following steps：On organic waste surface, sprinkling A liquid is sent out completely
Ferment heap is rotten, then sprays B liquid, is sufficiently mixed and mixes thoroughly, wherein, the organic waste is：Peat that volume ratio is 1: 1: 0.6~3,
Jig saw wood chip and barnyard manure, or volume ratio be 1: 1: 0.6~3 through crushing it is withered fall leaf branch, farmyard manure and barnyard manure, institute
It is the carbendazol wettable powder that mass ratio is 1: 150~250 and the solution that water is formed to state A liquid, and the B liquid is that volume ratio is 1:
The solution that 300 compound probiotic stoste and water is formed；
2) tissue-cultured seedling is transplanted
Knoxia valerianoide tissue-cultured seedling is washed into root with C liquid, is that Knoxia valerianoide tissue-cultured seedling is implanted in kind of a planting-line by 12~18cm by seeding row spacing
On so that the coring of Knoxia valerianoide tissue-cultured seedling completely attaches to the mixed planting matrix in kind planting-line, and sprays root water, wherein, institute
It is the carbendazol wettable powder that mass ratio is 1: 350~450 and the solution that water is formed to state C liquid.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, the step 1) also include before：
Site preparation plays furrow：Planting site trench plow is turned over, exposure 3~5 days, per 667m2Spread fertilizer over the fields 200~250kg of calcium magnesium phosphate, corruption
The ripe 500kg of organic fertilizer 1 000~1, rake is thin, mixes, and flattens furrow face, is 15~20cm by height, and width is that 1.5m plays furrow, and
When furrow are being built with firebrick.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, the step 2) are managed and protected after also including transplanting afterwards：
Step A, 12~18 days after transplanting, it is 28~32 DEG C to control the temperature in greenhouse, and relative humidity is 65~75%, light
Illumination is 1000~1200lx；
Step B, there is after seedling in Knoxia valerianoide tissue-cultured seedling, furrow face is being planted with withered grass, rice straw mulching, it is long to spring next year
Go out after sprouting that the withered grass of furrow face will be planted, straw is thrown off, Knoxia valerianoide medicinal material harvests after circulating step B to 3~4 years.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, in the step B, the withered grass, the equal nothing of the straw
Pest and disease damage.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, the step (2) are also needed Knoxia valerianoide tissue-cultured seedling before
Carry out hardening treatment.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, it is 1: 1: 3 that the raw material of the barnyard manure, which includes mass ratio,
Chicken manure, cow dung and the pig manure after methane-generating pit ferments.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, the hardening treatment are carried out in tissue culture bottle, specifically
For：Tissue culture bottle cap is opened, is placed in 30min under sunlight, is placed in indoor shady place 3 days, and by its in intensity be 80 micro- spies
30min is stood in this magnetic field drawn, 20min is stood in the infrared ray that wavelength is 900nm.
Preferably, described Knoxia valerianoide tissue-cultured seedling cultural method, in the step A, in addition to, in plantation 30 days
Afterwards, nutrient solution is applied to Knoxia valerianoide tissue-cultured seedling root, it is specially：
A, multiple a diameter of 1cm sponge ball is placed in nutrient solution and soaked, to each sponge ball in fill up the battalion
Nutrient solution, wherein, every 1 liter of nutrient solution places 100 sponge balls, the sponge ball is placed in refrigerator be iced in solid afterwards
State；
B, the sponge ball that frost handles to obtain in step a is placed in the heat-treated paraffin being in a liquid state so that described
Sponge ball is immersed in the paraffin of liquid, and is immediately taken out, and is cooled to room temperature, is obtained the paraffin sponge ball of solid-state, standby；
C, the paraffin sponge ball that step b is obtained is placed near Knoxia valerianoide tissue-cultured seedling root, when cultivating the 5th day, existed with pin
1/2 paraffin sponge ball surface stamp through hole, when cultivating the 20th day, through hole is stabbed in other 1/2 paraffin sponge ball surface with pin；
Wherein, the nutrient solution includes potassium chloride, potassium phosphate and the deep sea water that mass ratio is 19: 20: 500.
The present invention comprises at least following beneficial effect：
(1) planting soil for cultivating furrow face is respectively classified into thin river sand, mixed planting matrix, organic fertilizer mixture table soil from top to bottom
Three layers, the thin river sand in upper strata meets ventilative, permeable requirement；Many nation biological species of the middle level planting matrix addition containing organic matter and probiotics
Tailored version compound probiotic stoste is planted, is meeting that nutritional requirement is prebiotic on the basis of needed for ventilative, appropriate water conservation and plant growth
Bacterium can promote the activity of root, improve the survival rate of tissue culture transplantation of seedlings early stage, it is ensured that the survival rate in later stage phase；Bottom has containing abundance
Machine fertilizer and somewhat viscous, the further nutrient growth that more conducively Knoxia valerianoide root is passed through after middle level mixed planting matrix.
(2) cultural method of the present invention, due to matrix good air permeability, from transplanting to harvesting, do not have to dig again
Go out to preserve and plant again；And top layer is thin river sand, the weeds grown are less, save a large amount of man power and materials.
(3) the Knoxia valerianoide tissue-cultured seedling transplanting survival rate that is obtained using cultural method of the present invention and last plant into
Motility rate is high more than current general planting mode survival rate more than 95%；It is economic, practical, convenient, efficiently carry out tissue-cultured seedling
Artificial cultivation, efficiently solve the low bottleneck problem of the artificial cultivation scale survival rate of Knoxia valerianoide tissue-cultured seedling.
(4) nutrient solution is first drawn and stored by the present invention with sponge ball, and frost is got up so that as solid-state, then uses stone
The sponge ball for storing nutrient solution was fixed and sealed by wax, can be thus locked in nutrient solution inside paraffin ball, when cultivation 5 days
Afterwards, as cultivating process needs increasing nutrition, paraffin ball is now pricked into hole, the nutrient solution of inside is progressively discharged
Come, matched with incubation, reduce the evaporation loss of nutrient solution, make full use of nutritional ingredient, further increase utilization rate.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of individual other elements or its combination.
<Embodiment 1>
A kind of Knoxia valerianoide tissue-cultured seedling cultural method, comprises the following steps：
1) planting-line is planted to prepare
In furrow face, laying depth is 5cm mixed planting matrix successively from down to up, and the thin river sand structure that thickness is 3cm
Into kind of a planting-line；
Wherein, the mixed planting matrix is made by following steps：On organic waste surface, sprinkling A liquid is sent out completely
Ferment heap is rotten, then sprays B liquid, is sufficiently mixed and mixes thoroughly, wherein, the organic waste is：It is peat that volume ratio is 1: 1: 0.6, thin
Wood sawdust and barnyard manure, or volume ratio be 1: 1: 0.6 through crushing it is withered fall leaf branch, farmyard manure and barnyard manure, the A liquid
The carbendazol wettable powder and water that are 1: 150 for mass ratio form solution, the B liquid be volume ratio be 1: 300 it is compound
The solution that probiotics stoste and water are formed；
2) tissue-cultured seedling is transplanted
Knoxia valerianoide tissue-cultured seedling is washed into root with C liquid, is that Knoxia valerianoide tissue-cultured seedling is implanted on kind of planting-line by 12cm by seeding row spacing, makes
The coring and the mixed planting matrix in kind planting-line for obtaining Knoxia valerianoide tissue-cultured seedling completely attach to, and spray root water, wherein, the C liquid
The solution that the carbendazol wettable powder and water that are 1: 350 for mass ratio are formed.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step 1) also include before：
Site preparation plays furrow：Planting site trench plow is turned over, exposure 3 days, per 667m2Spread fertilizer over the fields calcium magnesium phosphate 200kg, decomposed manure
1 000kg, rake is thin, mixes, and flattens furrow face, is 15cm by height, and width is that 1.5m plays furrow, and when furrow are being built with firebrick.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step 2) are managed and protected after also including transplanting afterwards：
Step A, 12 days after transplanting, it is 28 DEG C to control the temperature in greenhouse, relative humidity 65%, and illuminance is
1000lx；
Step B, there is after seedling in Knoxia valerianoide tissue-cultured seedling, furrow face is being planted with withered grass, rice straw mulching, it is long to spring next year
Go out after sprouting that the withered grass of furrow face will be planted, straw is thrown off, Knoxia valerianoide medicinal material harvests after circulating step B to 3 years.
Described Knoxia valerianoide tissue-cultured seedling cultural method, in the step B, the withered grass, the equal no disease and pests harm of the straw.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step (2) also need Knoxia valerianoide tissue-cultured seedling carrying out hardening before
Processing.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the raw material of the barnyard manure include chicken manure, cow dung that mass ratio is 1: 1: 3
And the pig manure after methane-generating pit ferments.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the hardening treatment are carried out in tissue culture bottle, are specially：By tissue culture bottle
Bottle cap is opened, and is placed in 30min under sunlight, is placed in indoor shady place 3 days, and by it in the magnetic field that intensity is 80 micro- teslas
30min is stood, 20min is stood in the infrared ray that wavelength is 900nm.
Described Knoxia valerianoide tissue-cultured seedling cultural method, in the step A, in addition to, after plantation 30 days, to Knoxia valerianoide
Tissue-cultured seedling root applies nutrient solution, and it is specially：
A, multiple a diameter of 1cm sponge ball is placed in nutrient solution and soaked, to each sponge ball in fill up the battalion
Nutrient solution, wherein, every 1 liter of nutrient solution places 100 sponge balls, the sponge ball is placed in refrigerator be iced in solid afterwards
State；
B, the sponge ball that frost handles to obtain in step a is placed in the heat-treated paraffin being in a liquid state so that described
Sponge ball is immersed in the paraffin of liquid, and is immediately taken out, and is cooled to room temperature, is obtained the paraffin sponge ball of solid-state, standby；
C, the paraffin sponge ball that step b is obtained is placed near Knoxia valerianoide tissue-cultured seedling root, when cultivating the 5th day, existed with pin
1/2 paraffin sponge ball surface stamp through hole, when cultivating the 20th day, through hole is stabbed in other 1/2 paraffin sponge ball surface with pin；
Wherein, the nutrient solution includes potassium chloride, potassium phosphate and the deep sea water that mass ratio is 19: 20: 500.
<Embodiment 2>
A kind of Knoxia valerianoide tissue-cultured seedling cultural method, comprises the following steps：
1) planting-line is planted to prepare
In furrow face, laying depth is 7cm mixed planting matrix successively from down to up, and the thin river sand structure that thickness is 4cm
Into kind of a planting-line；
Wherein, the mixed planting matrix is made by following steps：On organic waste surface, sprinkling A liquid is sent out completely
Ferment heap is rotten, then sprays B liquid, is sufficiently mixed and mixes thoroughly, wherein, the organic waste is：Peat that volume ratio is 1: 1: 2, jig saw
Wood chip and barnyard manure, or volume ratio be 1: 1: 2 through crushing it is withered fall leaf branch, farmyard manure and barnyard manure, the A liquid is matter
Amount than for 1: 200 carbendazol wettable powder and water form solution, the B liquid be volume ratio be 1: 300 it is compound prebiotic
The solution that bacterium stoste and water are formed；
2) tissue-cultured seedling is transplanted
Knoxia valerianoide tissue-cultured seedling is washed into root with C liquid, is that Knoxia valerianoide tissue-cultured seedling is implanted on kind of planting-line by 15cm by seeding row spacing, makes
The coring and the mixed planting matrix in kind planting-line for obtaining Knoxia valerianoide tissue-cultured seedling completely attach to, and spray root water, wherein, the C liquid
The solution that the carbendazol wettable powder and water that are 1: 400 for mass ratio are formed.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step 1) also include before：
Site preparation plays furrow：Planting site trench plow is turned over, exposure 4 days, per 667m2Spread fertilizer over the fields calcium magnesium phosphate 220kg, decomposed manure
1230kg, rake is thin, mixes, and flattens furrow face, is 18cm by height, and width is that 1.5m plays furrow, and when furrow are being built with firebrick.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step 2) are managed and protected after also including transplanting afterwards：
Step A, 16 days after transplanting, it is 30 DEG C to control the temperature in greenhouse, relative humidity 70%, and illuminance is
1100lx；
Step B, there is after seedling in Knoxia valerianoide tissue-cultured seedling, furrow face is being planted with withered grass, rice straw mulching, it is long to spring next year
Go out after sprouting that the withered grass of furrow face will be planted, straw is thrown off, Knoxia valerianoide medicinal material harvests after circulating step B to 3 years.
Described Knoxia valerianoide tissue-cultured seedling cultural method, in the step B, the withered grass, the equal no disease and pests harm of the straw.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step (2) also need Knoxia valerianoide tissue-cultured seedling carrying out hardening before
Processing.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the raw material of the barnyard manure include chicken manure, cow dung that mass ratio is 1: 1: 3
And the pig manure after methane-generating pit ferments.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the hardening treatment are carried out in tissue culture bottle, are specially：By tissue culture bottle
Bottle cap is opened, and is placed in 30min under sunlight, is placed in indoor shady place 3 days, and by it in the magnetic field that intensity is 80 micro- teslas
30min is stood, 20min is stood in the infrared ray that wavelength is 900nm.
Described Knoxia valerianoide tissue-cultured seedling cultural method, in the step A, in addition to, after plantation 30 days, to Knoxia valerianoide
Tissue-cultured seedling root applies nutrient solution, and it is specially：
A, multiple a diameter of 1cm sponge ball is placed in nutrient solution and soaked, to each sponge ball in fill up the battalion
Nutrient solution, wherein, every 1 liter of nutrient solution places 100 sponge balls, the sponge ball is placed in refrigerator be iced in solid afterwards
State；
B, the sponge ball that frost handles to obtain in step a is placed in the heat-treated paraffin being in a liquid state so that described
Sponge ball is immersed in the paraffin of liquid, and is immediately taken out, and is cooled to room temperature, is obtained the paraffin sponge ball of solid-state, standby；
C, the paraffin sponge ball that step b is obtained is placed near Knoxia valerianoide tissue-cultured seedling root, when cultivating the 5th day, existed with pin
1/2 paraffin sponge ball surface stamp through hole, when cultivating the 20th day, through hole is stabbed in other 1/2 paraffin sponge ball surface with pin；
Wherein, the nutrient solution includes potassium chloride, potassium phosphate and the deep sea water that mass ratio is 19: 20: 500.
<Embodiment 3>
A kind of Knoxia valerianoide tissue-cultured seedling cultural method, comprises the following steps：
1) planting-line is planted to prepare
In furrow face, laying depth is 10cm mixed planting matrix successively from down to up, and the thin river that thickness is 3~5cm
Sand forms kind of a planting-line；
Wherein, the mixed planting matrix is made by following steps：On organic waste surface, sprinkling A liquid is sent out completely
Ferment heap is rotten, then sprays B liquid, is sufficiently mixed and mixes thoroughly, wherein, the organic waste is：Peat that volume ratio is 1: 1: 3, jig saw
Wood chip and barnyard manure, or volume ratio be 1: 1: 3 through crushing it is withered fall leaf branch, farmyard manure and barnyard manure, the A liquid is matter
Amount than for 1: 250 carbendazol wettable powder and water form solution, the B liquid be volume ratio be 1: 300 it is compound prebiotic
The solution that bacterium stoste and water are formed；
2) tissue-cultured seedling is transplanted
Knoxia valerianoide tissue-cultured seedling is washed into root with C liquid, is that Knoxia valerianoide tissue-cultured seedling is implanted on kind of planting-line by 18cm by seeding row spacing, makes
The coring and the mixed planting matrix in kind planting-line for obtaining Knoxia valerianoide tissue-cultured seedling completely attach to, and spray root water, wherein, the C liquid
The solution that the carbendazol wettable powder and water that are 1: 450 for mass ratio are formed.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step 1) also include before：
Site preparation plays furrow：Planting site trench plow is turned over, exposure 5 days, per 667m2Spread fertilizer over the fields calcium magnesium phosphate 250kg, decomposed manure
1 500kg, rake is thin, mixes, and flattens furrow face, is 20cm by height, and width is that 1.5m plays furrow, and when furrow are being built with firebrick.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step 2) are managed and protected after also including transplanting afterwards：
Step A, 18 days after transplanting, it is 32 DEG C to control the temperature in greenhouse, relative humidity 75%, and illuminance is
1200lx；
Step B, there is after seedling in Knoxia valerianoide tissue-cultured seedling, furrow face is being planted with withered grass, rice straw mulching, it is long to spring next year
Go out after sprouting that the withered grass of furrow face will be planted, straw is thrown off, Knoxia valerianoide medicinal material harvests after circulating step B to 4 years.
Described Knoxia valerianoide tissue-cultured seedling cultural method, in the step B, the withered grass, the equal no disease and pests harm of the straw.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the step (2) also need Knoxia valerianoide tissue-cultured seedling carrying out hardening before
Processing.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the raw material of the barnyard manure include chicken manure, cow dung that mass ratio is 1: 1: 3
And the pig manure after methane-generating pit ferments.
Described Knoxia valerianoide tissue-cultured seedling cultural method, the hardening treatment are carried out in tissue culture bottle, are specially：By tissue culture bottle
Bottle cap is opened, and is placed in 30min under sunlight, is placed in indoor shady place 3 days, and by it in the magnetic field that intensity is 80 micro- teslas
30min is stood, 20min is stood in the infrared ray that wavelength is 900nm.
Described Knoxia valerianoide tissue-cultured seedling cultural method, in the step A, in addition to, after plantation 30 days, to Knoxia valerianoide
Tissue-cultured seedling root applies nutrient solution, and it is specially：
A, multiple a diameter of 1cm sponge ball is placed in nutrient solution and soaked, to each sponge ball in fill up the battalion
Nutrient solution, wherein, every 1 liter of nutrient solution places 100 sponge balls, the sponge ball is placed in refrigerator be iced in solid afterwards
State；
B, the sponge ball that frost handles to obtain in step a is placed in the heat-treated paraffin being in a liquid state so that described
Sponge ball is immersed in the paraffin of liquid, and is immediately taken out, and is cooled to room temperature, is obtained the paraffin sponge ball of solid-state, standby；
C, the paraffin sponge ball that step b is obtained is placed near Knoxia valerianoide tissue-cultured seedling root, when cultivating the 5th day, existed with pin
1/2 paraffin sponge ball surface stamp through hole, when cultivating the 20th day, through hole is stabbed in other 1/2 paraffin sponge ball surface with pin；
Wherein, the nutrient solution includes potassium chloride, potassium phosphate and the deep sea water that mass ratio is 19: 20: 500.
Inventor additionally provide using current conventional method cultural method Knoxia valerianoide survival rate as a comparison case, and with this
The survival rate of the provided cultural method of invention is compared,
| Group | Comparative example | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Survival rate | 84% | 96% | 99% | 98% |
The Knoxia valerianoide tissue-cultured seedling transplanting survival rate that is obtained using cultural method of the present invention and last plant are survived
Rate is high more than current general planting mode survival rate more than 95%；It is economic, practical, convenient, efficiently carry out tissue culture the Miaos
Work is cultivated, and efficiently solves the low bottleneck problem of the artificial cultivation scale survival rate of Knoxia valerianoide tissue-cultured seedling.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the embodiment with description.
Claims (6)
1. a kind of Knoxia valerianoide tissue-cultured seedling cultural method, it is characterised in that comprise the following steps：
1) planting-line is planted to prepare
In furrow face, laying depth is 5~10cm mixed planting matrix successively from down to up, and the thin river that thickness is 3~5cm
Sand forms kind of a planting-line；
Wherein, the mixed planting matrix is made by following steps：On organic waste surface, sprinkling A liquid carries out complete fermentation heap
Corruption, then B liquid is sprayed, it is sufficiently mixed and mixes thoroughly, wherein, the organic waste is：Volume ratio is 1:1:0.6~3 peat, jig saw
Wood chip and barnyard manure, or volume ratio are 1:1:0.6~3 through crushing it is withered fall leaf branch, farmyard manure and barnyard manure, the A
Liquid is that mass ratio is 1:The solution that 150~250 carbendazol wettable powder and water is formed, the B liquid is that volume ratio is 1:300
Compound probiotic stoste and water form solution；
2) tissue-cultured seedling is transplanted
Knoxia valerianoide tissue-cultured seedling is washed into root with C liquid, is that Knoxia valerianoide tissue-cultured seedling is implanted on kind of planting-line by 12~18cm by line-spacing so that
The coring of Knoxia valerianoide tissue-cultured seedling completely attaches to the mixed planting matrix in kind planting-line, and sprays root water, wherein, the C liquid is
Mass ratio is 1:The solution that 350~450 carbendazol wettable powder and water is formed；
Managed and protected after also including transplanting after the step 2)：
Step A, 12~18 days after transplanting, it is 28~32 DEG C to control the temperature in greenhouse, and relative humidity is 65~75%, illuminance
For 1000~1200lx；
Step B, there is after seedling in Knoxia valerianoide tissue-cultured seedling, furrow face is being planted with withered grass and rice straw mulching, to spring next year, grow new
The withered grass for planting furrow face and straw are thrown off after bud, Knoxia valerianoide medicinal material harvests after circulating step B to 3~4 years；
In the step A, in addition to, apply nutrient solution to Knoxia valerianoide tissue-cultured seedling root, it is specially：
A, multiple a diameter of 1cm sponge ball is placed in nutrient solution and soaked, to each sponge ball in fill up the nutrient solution,
Wherein, every 1 liter of nutrient solution places 100 sponge balls, the sponge ball is placed in refrigerator be iced in solid-state afterwards；
B, the sponge ball that frost handles to obtain in step a is placed in the heat-treated paraffin being in a liquid state so that the sponge
Ball is immersed in the paraffin of liquid, and is immediately taken out, and is cooled to room temperature, is obtained the paraffin sponge ball of solid-state, standby；
C, the paraffin sponge ball that step b is obtained is placed near Knoxia valerianoide tissue-cultured seedling root, when cultivating the 5th day, with pin 1/2
Paraffin sponge ball surface stabs through hole, and when cultivating the 20th day, through hole is stabbed in other 1/2 paraffin sponge ball surface with pin；
Wherein, it is 19 that the nutrient solution, which includes mass ratio,:20:500 potassium chloride, potassium phosphate and deep sea water.
2. Knoxia valerianoide tissue-cultured seedling cultural method as claimed in claim 1, it is characterised in that also include before the step 1)：
Site preparation plays furrow：Planting site trench plow is turned over, exposure 3~5 days, per 667m2Spread fertilizer over the fields 200~250kg of calcium magnesium phosphate and decomposed have
Machine 1 000~1 500kg of fertilizer, rake is thin, mixes, and flattens furrow face, is 15~20cm by height, and width is that 1.5m plays furrow, and in furrow
When being built with firebrick.
3. Knoxia valerianoide tissue-cultured seedling cultural method as claimed in claim 1, it is characterised in that in the step B, the withered grass,
The equal no disease and pests harm of straw.
4. Knoxia valerianoide tissue-cultured seedling cultural method as claimed in claim 1, it is characterised in that also need before the step (2) by
Knoxia valerianoide tissue-cultured seedling carries out hardening treatment.
5. Knoxia valerianoide tissue-cultured seedling cultural method as claimed in claim 1, it is characterised in that the raw material of the barnyard manure includes quality
Than for 1:1:3 chicken manure, cow dung and the pig manure after methane-generating pit ferments.
6. Knoxia valerianoide tissue-cultured seedling cultural method as claimed in claim 4, it is characterised in that the hardening treatment is in tissue culture bottle
Carry out, be specially：Tissue culture bottle cap is opened, is placed in 30min under sunlight, is placed in indoor shady place 3 days, and by it in intensity
30min is stood in magnetic field for 80 micro- teslas, 20min is stood in the infrared ray that wavelength is 900nm.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766086A (en) * | 2009-01-04 | 2010-07-07 | 王兴祥 | Method for rapidly cutting and reproducing euphorbia pekinensis |
| CN103477901A (en) * | 2013-07-29 | 2014-01-01 | 广西植物组培苗有限公司 | Nutritive cup provisional-planting substrate and provisional-planting method for tissue culture seedlings of downy grapes |
| CN104186175A (en) * | 2014-09-03 | 2014-12-10 | 浙江农林大学 | Underwood planting method of anoectochilus roxburghii |
| CN104541904A (en) * | 2015-01-23 | 2015-04-29 | 陕西省西安植物园 | Field human assistance overwintering method for barbadoslily |
| CN104604510A (en) * | 2015-02-04 | 2015-05-13 | 长沙山湘农产品开发有限公司 | Anoectochilus formosanus planting method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10313717A (en) * | 1997-05-15 | 1998-12-02 | Nikka Chem Co Ltd | Tomato seed and seedling culturing method |
-
2015
- 2015-11-20 CN CN201510810887.5A patent/CN105265159B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766086A (en) * | 2009-01-04 | 2010-07-07 | 王兴祥 | Method for rapidly cutting and reproducing euphorbia pekinensis |
| CN103477901A (en) * | 2013-07-29 | 2014-01-01 | 广西植物组培苗有限公司 | Nutritive cup provisional-planting substrate and provisional-planting method for tissue culture seedlings of downy grapes |
| CN104186175A (en) * | 2014-09-03 | 2014-12-10 | 浙江农林大学 | Underwood planting method of anoectochilus roxburghii |
| CN104541904A (en) * | 2015-01-23 | 2015-04-29 | 陕西省西安植物园 | Field human assistance overwintering method for barbadoslily |
| CN104604510A (en) * | 2015-02-04 | 2015-05-13 | 长沙山湘农产品开发有限公司 | Anoectochilus formosanus planting method |
Non-Patent Citations (1)
| Title |
|---|
| 红芽大戟组培苗移栽技术的研究;黄浩等;《热带农业科技》;20070331;第30卷(第3期);第27-28、31页 * |
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Application publication date: 20160127 Assignee: Guangxi Weisen Biotechnology Group Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980045127 Denomination of invention: Cultivation Method of Red Euphorbia Tissue Culture Seedlings Granted publication date: 20171212 License type: Common License Record date: 20231101 |