CN108684460A - Radix knoxiae cultural method - Google Patents

Abstract

The invention discloses a method for cultivating Euphorbia euphorbius, comprising: planting propagation materials into light substrate cups to obtain cup seedlings, acclimatizing and cultivating the cup seedlings and transplanting them to the field, undergoing field management, overwintering management and After germination in spring, culture until harvest; wherein, the light matrix cup is made of base material, and the base material includes the following raw materials in parts by weight: 60-70 parts of peat soil, 5-8 parts of coconut peat, 5 parts of perlite ~8 parts, humic acid phosphate fertilizer 4~5 parts, yellow mud 10~15 parts, floating beads 6~8 parts, carbonized rice husk 10~15 parts. Compared with the prior art, the present invention can effectively avoid the problem that the survival rate is affected by transplanting, facilitates the heat preservation of potato pieces in winter and improves the germination rate in spring. The final yield of Euphorbia euphorbia cultivated by the method disclosed in the invention is obviously improved.

Description

Cultivation method of Euphorbia euphorbia

technical field

The invention belongs to the technical field of agricultural planting, and in particular relates to a method for cultivating Euphorbia red buds.

Background technique

Red Euphorbia (Knoxia valerianoides Thorel.) is a perennial herbaceous plant of the genus Knoxia valerianoides in Rubiaceae. It is distributed in the southern provinces of my country. The medicinal part of Euphorbia euphorbia is the root, which has the effects of diuresis, detumescence, and stagnation. It is the main drug of Zijinding, a Chinese patent medicine, and the main ingredient of the main product "Tianhe Zhuifeng Ointment" produced by Guilin Tianhe Pharmaceutical Co., Ltd.

Over the years, the required medicinal materials have been obtained from wild resources. However, due to the vegetation degradation in the original habitat of the red euphorbia, excessive artificial harvesting and digging, and the widespread use of herbicides, the number of wild resources has decreased sharply. The germination rate is low, the growth rate is slow, and wild medicinal materials are far from meeting people's needs.

As early as 1989, Pan Jichang introduced and cultivated by seeds and split-root propagation (Pan Jichang, Introduction and Cultivation Technique of Euphorbia euphorbia); after 1993, many researchers made great breakthroughs in the propagation of Euphorbia euphorbia by means of biotechnology (Huang euphorbia Hao, research on tissue culture of medicinal plant Euphorbia red bud; Ling Zhengzhu et al., Regenerated plants obtained from tissue culture of red Euphorbia; Chen Fangqing et al. Tissue culture of medicinal plant Euphorbia red bud; Qiu Anjing et al. tissue culture), but the transplanting and cultivation survival rate of tissue cultured seedlings is still a bottleneck that limits artificial planting, and the highest transplanting survival rate only reaches 88.36% (Huang Hao et al. Research). The invention with the patent application number CN201510810887.5 discloses a method for cultivating Euphorbia tissue-cultivated seedlings. Compared with the prior art, the transplanting survival rate and final plant survival rate of Euphorbia tissue-cultivated seedlings obtained by the cultivation method are The rate is more than 95%, which basically effectively solves the bottleneck problem of low survival rate of large-scale artificial cultivation of Euphorbia euphorbia tissue culture seedlings. However, in this method, the tissue cultured seedlings are directly transplanted to the planting bed, which not only requires careful operation, but also has high requirements for the planting bed, and it is troublesome to reuse the planting bed, and the seedlings need to adapt to the environment of the planting bed again. , even if the seedlings can survive, the roots are often easily rotted during the winter and affect the growth of the next year. In the past, in order to ensure the yield, it was often necessary to dig out the potato roots in winter during artificial planting, bury them in ventilated, moist sand or sawdust in the room or in a kiln, and replant the potato roots in the next year. The amount is large. In the invention with the patent application number CN201510810887.5, after the downed seedlings of Euphorbia euphorbia tissue culture seedlings appear, the withered grass and straw are used to cover the planting border. In the spring of the following year, the withered grass and straw on the border will be planted after new shoots grow. Remove and repeat this step until 3 to 4 years after the harvest of the Euphorbia euphorbia medicinal material, although a certain effect has been achieved, the process is cumbersome and labor-intensive; at the same time, it is difficult to drain water when there is a lot of rain, resulting in some euphorbia euphorbia Although it can germinate, the roots are rotten due to too much water and cannot be used. In the end, the harvest of Euphorbia euphorbia is not optimistic.

Contents of the invention

The object of the present invention is to solve at least the above-mentioned disadvantages and to provide advantages which will be explained later.

In order to achieve these objects and other advantages of the present invention, a method for cultivating Euphorbia euphorbia is now provided, by planting the propagating material into light substrate cups to obtain cup seedlings, and transplanting the cup seedlings to the field after domestication and cultivation , after field management, overwintering management and germination in spring, cultivate until harvest. Reduce the damage to the plants during the transplanting process, which is conducive to rapid growth in the second year, not only increasing the output but also reducing labor.

In other words, the present invention discloses a method for cultivating Euphorbia euphorbia, comprising:

Plant the propagating material into light substrate cups to obtain cup seedlings, transplant the cup seedlings to the field after acclimatization and cultivation, undergo field management, overwintering management and spring germination, and cultivate until harvesting; wherein, the light substrate cups It is prepared by using a base material, and the base material includes the following raw materials in parts by weight: 60-70 parts of peat soil, 5-8 parts of coconut peat, 5-8 parts of perlite, 4-5 parts of humic acid phosphate fertilizer, and 10 parts of yellow mud. ～15 parts, 6～8 parts of floating beads, 10～15 parts of carbonized rice husk. The light substrate cup made has good air permeability, and can increase the organic matter, humus and microbial quantity of the soil, increase the structure of the soil mass, improve the fertility and quality of the soil, and create an excellent environment for the growth of Euphorbia.

Preferably, the base material is mixed evenly and distributed into non-woven bags to make the light matrix cup, and then the light matrix cup is placed on the empty tray of the seedling raising rack and covered with a film for future use. The use of non-woven bags can achieve the purpose of moisture-proof, so that when the rain is too full, the water in the planting ground cannot overflow into the substrate cup. At the same time, the non-woven bag has good air permeability, which is beneficial to the propagation of the material and drains the water out of the substrate. cup.

Preferably, the propagation material is prepared by the following method: after hardening the Euphorbia tissue cultured seedlings for 30 days, the medium at the base is washed away to obtain seedlings, and the seedlings are the propagation materials. Wash away the medium at the base to prevent the growth of bacteria that can cause root rot.

Preferably, the seedlings are transplanted into the light matrix cup, and sprayed with water to fix the roots, and after spray disinfection, cover with a film to keep the seedlings in the cup.

Preferably, the domestication and cultivation of the cup seedlings specifically includes: disinfecting the cup seedlings on the 3rd day after obtaining the cup seedlings, then disinfecting once a week, spraying water-soluble fertilizer after 10 days, and transplanting out of the nursery after 90 days Daejeon operation.

Preferably, the transplanting field operation includes the following steps: deeply plowing the planting site, spreading fertilizer after 10 days of exposure to the sun, raking the planting site, mixing the fertilizer and leveling the border surface, with a height of 20 cm and a width of 1.5 m Raise the furrow, make drainage ditches and form the field with red bricks on the edge of the furrow; transplant the cup seedlings after acclimatization and cultivation to the field according to the specifications of 10cm×10cm, cover with soil and pour enough water to set the field. Root and plant two rows of corn on the border.

Preferably, the field management includes the following steps: 1-7 days after the cup seedlings are transplanted to the field, keep water and moisturize, 10 days after normal management, and 25-30 days later, water urea or compound fertilizer aqueous solution.

Preferably, the overwintering management includes: before the temperature drops below 10 degrees in winter, cover the field with a black plastic net to keep warm, and prohibit artificial watering. The black plastic net absorbs heat during the day and heats it up, and it can keep warm at night; when there is a lot of rain, it is also convenient for drainage and ventilation, and prevents the roots of the seedlings from rotting due to rainwater accumulating on the seedlings.

Preferably, the germination in spring includes: after the temperature is stabilized above 18°C in the next year, remove the black plastic net, and spray EM bacteria solution for germination; ready to harvest.

Preferably, the light matrix cup is thoroughly disinfected with potassium permanganate aqueous solution 1 day before transplanting; the seedlings are disinfected by soaking in potassium permanganate aqueous solution for 5 minutes before transplanting. The double disinfection of plants and light matrix cups reduces the occurrence of diseases, prepares for the subsequent domestication of seedlings, and at the same time avoids the phenomenon of rot before the wounds heal, and improves the survival rate.

The conditions for hardening the Euphorbia euphorbia tissue culture seedlings are as follows: cover with a shade net and the shading degree is not less than 70%, the color of the shade net is green, the humidity in the shade net is 80-100%, and the shade inside the shade net The temperature is 25-28°C; simulate the harsh environment to give the seedlings an adaptation period, improve the adaptability of the seedlings to the environment, improve the photosynthetic ability of the seedlings, promote the robustness of the tissue-cultured seedlings, and finally achieve the purpose of transplanting and surviving the tissue-cultured seedlings. The seedling hardening is carried out in a tissue culture bottle, specifically: open the bottle mouth of the tissue culture bottle, place it in a cool place indoors for 3 days, then place it in a magnetic field with an intensity of 80 microtesla for 30 minutes, and then place it in a wavelength Infrared rays of 900nm for 20 minutes, transitional exercise for seedlings, to enhance the adaptability of seedlings to the environment.

In the domestication and cultivation of cupped seedlings, the water-soluble fertilizer is 1-1.5wt‰ mineral fulvic acid aqueous solution, which not only plays an important role in drought resistance, but also improves plant stress resistance and effectively promotes the growth of Radix euphorbia.

The cup seedlings that can be out of the nursery are sprayed with a plant anti-transpiration agent before transplanting to the field. The plant anti-transpiration agent is an aqueous solution of a powder-type anti-transpiration agent diluted by 6000 to 8000 times. The plant anti-transpiration agent can Close the stomata on the plant surface, and form an ultra-thin light-transmitting protective film on the plant branches and leaf surfaces, effectively inhibit excessive transpiration of water in the plant, minimize damage caused by transplantation, drought and wind erosion, and delay metabolism. The mesh structure and its molecular gap are breathable, which can ensure the normal breathing and ventilation of plants and improve the survival rate.

In the field transplanting operation, the fertilization is specifically: 200 kg of calcium magnesium phosphate fertilizer and 1000 kg of decomposed organic fertilizer per 667 square meters of planting land. To provide the required nutrients for the growth of Euphorbia halberds, reasonable fertilization can not only supply sufficient nutrients and avoid waste, but also avoid crop poisoning caused by excessive fertilizer residues and affect the survival rate of crops.

The EM bacterial liquid adopts 0.5% EM fermentation dilution. EM bacteria are very easy to survive and reproduce in the soil, so they can quickly and stably occupy the ecological position in the soil, forming a dominant community of beneficial microbial bacteria, thereby controlling the reproduction of pathogenic microorganisms and the invasion of crops. After dilution, EM The bacterial liquid is sufficient to meet the effect of anti-bacteria and germination acceleration.

Advantages of the present invention:

1. The base material is mixed to make a light matrix cup, which not only meets the nutrients needed for the growth of the red euphorbia seedlings, but also has better ventilation and water permeability performance, creating a good environment for the growth of the red euphorbia seedlings. surroundings.

2. The domestication of euphorbia seedlings is carried out directly in light substrate cups, and the rapid growth of roots of euphorbia seedlings is promoted by spraying water-soluble fertilizers, and then the domesticated cup seedlings are directly transplanted to the planting site to reduce the risk of transplantation. The damage to the plants during the planting process affects the survival rate, and is conducive to the rapid growth of the second year, which not only increases the output but also reduces labor.

3. Field finishing according to certain specifications will help the red euphorbia better absorb nutrients during the growth process, and ensure that there are enough gaps between the red euphorbia plants to satisfy each seedling with smooth ventilation And avoid the rotten potato pieces.

4. Reasonable overwintering maintenance, use black plastic net to cover for heat preservation, it is very convenient to cover and uncover, reduce labor and ensure that the seedlings will not be frostbitten in the cold winter and affect the survival rate.

5. Germination by spraying EM bacteria solution can improve the soil environment, and can effectively control the reproduction of pathogenic microorganisms and the invasion of crops, thereby promoting faster germination of Euphorbia seedlings; moreover, the metabolism of the flora The product can also be directly absorbed by the Euphorbia spp. seedlings to provide nutrients for the growth of Euphorbia spp.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below, so that those skilled in the art can implement it by referring to the description. However, it should be noted that many of the details listed in the specification are only for readers to have a thorough understanding of one or more aspects of the present invention, and these aspects of the present invention can be implemented even without these specific details.

Example 1

A method for domesticating and planting red euphorbia tissue culture seedlings, specifically comprising the following steps:

Step 1. Production and loading of light substrate cups: Take 60 parts of peat soil, 5 parts of coconut peat, 5 parts of perlite, 4 parts of humic acid phosphate fertilizer, 10 parts of yellow mud, 6 parts of floating beads and 10 parts of carbonized rice husk After mixing, the mixed base material is divided into non-woven bags to make light substrate cups, and the prepared light substrate cups are placed on the empty tray of the seedling raising rack, covered with a film for subsequent use.

Step 2, cover the red euphorbia tissue culture seedlings with a green shade net with a shading degree of 70%, and adjust the humidity in the shade net to be 80%, and the temperature is 25°C to harden the seedlings for 30 days, and then wash away the cultivation of the base Base, get seedlings.

Step 3. Transplant the tissue-cultured seedlings to the light matrix cup: sterilize the light matrix cup described in step 1 with potassium permanganate aqueous solution 1 day before transplanting, soak the seedlings in step 2 in potassium permanganate The aqueous solution is sterilized for 5 minutes, and then transplanted into a sterilized light substrate cup, and sprayed with water to fix the roots, and after spray disinfection, cover with a film to moisturize, and obtain a cup seedling.

Step 4, post-acclimatization management: The cup seedlings are sterilized for the second time on the third day, and then once a week, and spray 1wt‰ mineral fulvic acid water-soluble fertilizer after the 10th day, and can be out of the nursery on the 95th day.

Step 5. Field finishing and transplanting: plow the planting ground deeply, and after 10 days of exposure, spread 200kg of calcium magnesium phosphate fertilizer and 1000kg of decomposed organic fertilizer per 667 square meters, and rake the planting ground to mix the fertilizer and level the border On the surface, be 20cm in height and 1.5m in width to start the furrow, make drainage ditches and build edges with red bricks at the edge of the furrow; the cup seedlings that can go out of the garden described in step 4 are sprayed on the seedlings before being transplanted to the field. The aqueous solution of the powdered anti-transpiration agent diluted 6000 times was then transplanted to the field according to the size of 10cm×10cm, covered with 3cm of soil and poured with enough water to set the roots and planted two rows of corn on the edge of the border.

Step 6. Field management: 1 to 7 days after transplanting to the field, water must be kept and moisturized. After 10 days, it should be managed normally, and urea should be poured after 25 to 30 days.

Step 7. Overwintering management: Before the temperature drops below 10 degrees in winter, the red euphorbia seedlings are laid down, covered with black plastic nets to keep warm, and artificial watering is prohibited.

Step 8. Spring germination: After the temperature is stable above 18 degrees in the next year, remove the black plastic net, spray 0.5% EM fermentation dilution to accelerate germination, manage normally after new shoots grow, and harvest in winter in the third year.

Example 2

A method for domesticating and planting red euphorbia tissue culture seedlings, specifically comprising the following steps:

Step 1. Production and loading of light substrate cups: Take 70 parts of peat soil, 8 parts of coconut peat, 8 parts of perlite, 5 parts of humic acid phosphate fertilizer, 15 parts of yellow mud, 8 parts of floating beads and 15 parts of carbonized rice husk After mixing well, the mixed base material is subpackaged to obtain a light matrix cup, which is placed on an empty plate of a seedling raising rack and covered with a film for subsequent use.

All the other parameters are identical with embodiment 1, and technological process is also identical.

Comparative example 1

A method for cultivating Euphorbia euphorbia tissue culture seedlings, comprising the following steps:

Step 1. Soil preparation and furrowing: plow and plow the planting land, expose to the sun for 3 days, spread 200kg of calcium magnesium phosphate fertilizer and 1,000kg of decomposed organic fertilizer per 667m2, rake finely, mix well, and level the furrow surface, according to the height of 15cm, width Build a furrow for 1.5m, and use fire bricks to build the edge of the furrow.

Step 2. Preparation of mixed planting substrate: Spray liquid A on the surface of the organic waste for complete fermentation and composting, then spray liquid B, mix well, wherein the organic waste is: the volume ratio is 1:1:0.6 Peat, fine sawdust and manure, or crushed littered leaves and branches, soil manure and manure with a volume ratio of 1:1:0.6, and the A liquid is carbendazim wettable with a mass ratio of 1:150 A solution composed of powder and water, the B liquid is a solution composed of a composite probiotic stock solution and water with a volume ratio of 1:300; wherein, the raw materials of the manure include chicken manure, cow manure, and cattle manure with a mass ratio of 1:1:3. Manure and pig manure fermented by biogas tanks.

Step 3, planting furrow preparation: Lay a mixed planting substrate with a thickness of 5cm on the furrow surface from bottom to top, and fine river sand with a thickness of 3cm to form the planting furrow;

Step 4, the tissue-cultured seedlings of Euphorbia euphorbia are subjected to hardening treatment. Wherein, the seedling hardening treatment is carried out in a tissue culture bottle, specifically: open the bottle cap of the tissue culture bottle, place it in the sun for 30 minutes, and place it in a cool place indoors for 3 days, and place it in a temperature of 80 microtes Stand still for 30 minutes in a pulled magnetic field, and stand still for 20 minutes in infrared rays with a wavelength of 900nm.

Step 5, tissue-cultured seedling transplantation: wash the roots of the red euphorbia tissue-cultured seedlings with C liquid, and transplant the red euphorbia tissue-cultured seedlings on the planting bed according to the row spacing of 12 cm, so that the roots of the red euphorbia tissue-cultured seedlings are in line with the planting The mixed planting substrate in the furrow is completely in contact with, and the root water is sprayed, wherein the liquid C is a solution composed of carbendazim wettable powder and water with a mass ratio of 1:350.

Step 6. Care after transplantation:

Step A, 12 days after transplantation, the temperature in the control greenhouse is 28°C, the relative humidity is 65%, and the illuminance is 1000lx;

After planting 30 days, apply nutrient solution to the roots of Euphorbia euphorbia tissue culture seedlings, which are specifically:

a, a plurality of sponge balls with a diameter of 1 cm are placed in the nutrient solution and soaked until each sponge ball is filled with the nutrient solution, wherein, 100 of the sponge balls are placed for every 1 liter of the nutrient solution, and then all the sponge balls are placed The sponge ball is placed in the refrigerator and frozen until it is solid state;

b. Place the sponge ball obtained by the freezing treatment in step a into the liquid paraffin wax after heating treatment, so that the sponge ball is immersed in the liquid paraffin wax, and take it out immediately, and cool to room temperature to obtain a solid paraffin wax sponge ball, spare;

c. Place the paraffin sponge ball obtained in step b near the roots of the Euphorbia tissue culture seedlings. On the 5th day of cultivation, use a needle to poke through holes on the surface of 1/2 of the paraffin sponge ball. 1/2 of the surface of the paraffin sponge ball poke through holes;

Wherein, the nutrient solution includes potassium chloride, potassium phosphate and deep seawater in a mass ratio of 19:20:500.

Step B. After the red euphorbia tissue culture seedlings have fallen seedlings, cover the planting border with withered grass and straw until the next spring. After new shoots grow, remove the withered grass and straw on the planting border, and repeat steps B to 3 Red Euphorbia medicinal materials are harvested after the year.

Wherein, in step B, neither the subtilized grass nor the rice straw is damaged by diseases and insect pests.

Cultivate 1000 plants of Euphorbia euphorbia seedlings respectively by the method of embodiment and comparative example, compare the survival situation of Euphorbia euphorbia plants in the winter of the first year, the second year and the third year respectively, the results are shown in Table 1:

project Example 1 Example 2 Comparative example 1 first year survival rate 986 strains 990 plants 949 strains second year survival rate 980 plants 982 strains 800 plants third year survival rate 976 strains 979 strains 652 strains

From the data obtained in Table 1, it can be clearly seen that the Euphorbia euphorbia planted by the method of Example 1 and Example 2 is hardly affected by the harsh environment in winter, while the Euphorbia euphorbia planted by the method of Comparative Example 1 Although the survival rate in the first winter was relatively high, the survival rate of the plants decreased significantly after the second year.

After counting the survival situation of the plants in the winter of the third year, harvest the Euphorbia euphorbia cultivated by the method of the embodiment and the comparative example respectively, and the results are shown in Table 2:

project Example 1 Example 2 Comparative example 1 good number of tubers 976 strains 979 strains 361 strains

By the data obtained in Table 2, it can be clearly seen that the Euphorbia euphorbia not only has a high survival rate, but the roots obtained by planting the method of Example 1 and Example 2 are intact without rot. rate reaches 98%; and though the method of comparative example 1 planted the resulting Euphorbia erythrophylla, the survival rate was 65%, but in the surviving plants, 45% of the plant tubers had rotted and could not be used. Actual availability is only 36%.

Although embodiments of the present invention have been disclosed above, it is not limited to the applications set forth in the specification and examples. It can be fully applied to various fields suitable for the present invention. Additional modifications can readily be made by those skilled in the art.

Claims (10)

1. a kind of radix knoxiae cultural method, which is characterized in that including:

Cup seedling will be obtained into propagating materials plantation to Light media cup, by transplanting after the domestication culture at cup seedling to crop field, warp After going through field management, overwinter management and spring vernalization, culture is until harvest;

Wherein, the Light media cup is made using base-material, and the base-material includes the raw material of following parts by weight:Peat soil 60~70 Part, 5~8 parts of coconut palm chaff, 5~8 parts of perlite, 4~5 parts of humic acid phosphate fertilizer, 10~15 parts of yellow mud, 6~8 parts of floating bead, charring rice husk 10~15 parts.

2. radix knoxiae cultural method as described in claim 1, which is characterized in that by the base-material dispense after mixing to The Light media cup is made in non-woven bag, it is standby then to be covered into film on the blank panel of seedling culture frame for manufactured Light media cup With.

3. radix knoxiae cultural method as described in claim 1, which is characterized in that the propagating materials is made by the following method It is standby:

By Knoxia valerianoide tissue-cultured seedling after hardening 30 days, the culture medium of base portion is washed away, obtains seedling, the seedling is described numerous Grow material.

4. radix knoxiae cultural method as claimed in claim 3, which is characterized in that by the seedling replanting to the Light media It in cup, and sprays clear water and determines root, thin film moisturizing is covered after spraying disinfection, obtains into cup seedling.

5. radix knoxiae cultural method as claimed in claim 4, which is characterized in that described at the specific packet of cup seedling domestication culture It includes:

The carrying out disinfection for the 3rd day at cup seedling is being obtained, is sterilizing 1 time weekly later, Water soluble fertilizer is sprayed after 10 days, after 90 days It can be out of the garden and carry out transplanting field practice.

6. radix knoxiae cultural method as claimed in claim 5, which is characterized in that the transplanting field practice includes following step Suddenly:

Planting site trench plow is turned over, broadcast application after exposure 10 days, planting site rake carefully by fertilizer mixing and flattens furrow face, by being highly 20cm, width are that 1.5m plays furrow, carry out gutter and form the crop field when being built with common brick in furrow;According to 10cm × 10cm's Specification will be after domestication culture at cup transplantation of seedlings to the crop field, earthing simultaneously spoons enough water and determine root and in furrow side plantation two rows Corn.

7. radix knoxiae cultural method as claimed in claim 6, which is characterized in that the field management includes the following steps:

Water conservation moisturizing in 1~7 day after the transplantation of seedlings to crop field at cup, normally manages and protects after 10 days, is poured after 25~30 days and apply urine Element or composite fertilizer's aqueous solution.

8. radix knoxiae cultural method as claimed in claim 7, which is characterized in that the overwinter management includes:In winter temperature Before degree drops to 10 degree or less, it is covered on crop field and is kept the temperature with black plastic net, and forbid artificial water drenching.

9. radix knoxiae cultural method as claimed in claim 8, which is characterized in that the spring vernalization includes:Wait for next year gas Temperature is stablized after 18 DEG C or more, and black plastic net is thrown off, and sprays EM bacterium solutions and carries out vernalization;And normal management is trained after growing sprouting It supports, the 3rd year autumn or winter can harvest.

10. radix knoxiae cultural method as claimed in claim 9, which is characterized in that the Light media cup is 1 day before transplanting It is irrigated and is carried out disinfection with potassium permanganate solution;The seedling is soaked in potassium permanganate solution before transplanting and sterilizes 5 minutes;

The condition of the hardening of the Knoxia valerianoide tissue-cultured seedling is:It is covered with shady net and degree of shading is not less than the color of 70%, shady net Humidity in green, shady net be 80~100% and shady net in temperature be 25~28 DEG C;The hardening in tissue culture bottle into Row, specially:The bottleneck of tissue culture bottle, shady place disposed within 3 days are opened, then is statically placed in the magnetic field that intensity is 80 micro- teslas 30min, then it is placed in 20min in the infrared ray that wavelength is 900nm;

In the domestication culture at cup seedling, the Water soluble fertilizer is the mineral yellow corruption aqueous acid of 1~1.5wt ‰;

What can be out of the garden steams seedling spray on plants antitranspirant, the Genes For Plant Tolerance before carrying out transplanting field practice at cup seedling It is aqueous solution of the powder-type antitranspirant after 6000~8000 times of dilutions to rise agent;

In the transplanting field practice, the broadcast application is specially:Every 667 square metres of planting sites spread fertilizer over the fields calcium magnesium phosphate 200kg and Decomposed manure 1000kg;

The EM bacterium solutions are using 0.5%EM fermentation dilutions.