CN102144526A - Detoxified seedling stem matrix floating seedling culturing method for potato - Google Patents

Detoxified seedling stem matrix floating seedling culturing method for potato Download PDF

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CN102144526A
CN102144526A CN2011100000972A CN201110000097A CN102144526A CN 102144526 A CN102144526 A CN 102144526A CN 2011100000972 A CN2011100000972 A CN 2011100000972A CN 201110000097 A CN201110000097 A CN 201110000097A CN 102144526 A CN102144526 A CN 102144526A
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seedling
matrix
days
seedlings
cuttage
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包丽仙
杨琼芬
卢丽丽
李先平
尹自友
白建明
徐宁生
李山云
李燕山
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INSTITUTE OF INDUSTRIAL CROP YUNNAN ACADEMY OF AGRICULTURAL SCIENCES
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Abstract

The invention provides a detoxified seedling stem matrix floating seedling culturing method for potato, comprising the following steps of: shearing a detoxified tissue culture seedling into stem sections, wherein each stem section is between two joints; processing the stem sections by using root-growing liquid containing 100 PPm of naphthylacetic acid and 50 PPm of farm-oriented streptomycin, cutting the stem sections in a plastic foam seedling culture disc, wherein the plastic foam seedling culture disc contains a matrix consisting of perlite and grass carbon and the ratio of perlite to grass carbon is 1:1; placing the plastic foam seedling culture disc with the stem sections in a sterilized water slot for culturing; shielding during the early stage of culturing, spraying chemical agents during the middle stage for preventing late blight, pumping water out competely at the rear stage and acclimatizing seedlings for 12-20 days to obtain strong seedlings which can be directly transplanted in field. The seedling culturing method is used in the following three aspects: 1, the method can be used for producing the seedlings used for breeding breeder stock in a large scale, has higher fixed planting survival rate than a method of directly planting tissue culture seedlings, the cultured seedlings grow uniformly and are easy to manage, production efficiency of the breeder stock per unit area is high; 2, the method can be used for producing and providing seedlings used for directly breeding breeder seed in the field, can reduce the step of producing micro potatoes in a greenhouse, thereby reducing the cost of breeder seed production process and shortening the production time of the detoxified potato seeds; and 3, the method can be used for producing and providing the seedlings used for gas-culturing and breeding the breeder stock, and is convenient for transplanting and managing.

Description

A kind of potato virus-free plantlet stem segment base matter floating seedlings culturing method
Technical field
The technology of the present invention method relates to a kind of novel seedling growing process of potato virus-free plantlet stem segment base matter method floating seedlings, and the germchit of potato of taking off poison that being used for scale provides field planting to produce original seed, mist training production breeder's stock is grown seedlings.
Background technology
China's virus-free seed potato generally adopts Pyatyi potato seed breeding of method, in recent years, for quality that improves virus-free seed potato and the popularization degree that improves virus-free seed potato, carries out three grades of potato seed systems.More than two kinds of breedings of method, (or two produce seasons) just can obtain the potato seeds of a large amount of plantations in the land for growing field crops all will to need the time more than 2 years through the production process of " core detoxic seedling---breeder's stock---original seed ".Breeder's stock and original seed production link are the highest links of cost during whole potato seed is produced.In order to accelerate the potato seed reproduction speed, reduce the production cost of potato seed, the production unit that has adopts the method for the direct field planting of detoxic seedling to land for growing field crops production potato seed, but also exists survival rate low, and growing way is irregular, and the management recruitment is many, problems such as inconvenient operation.Publication number is that the patent of invention of CN101138310 discloses a kind of method with detoxicating cuvette potato float seedling, and the material of employing is a detoxicating cuvette potato.About the stem section that adopts detoxic seedling in matrix with the technology of floating method scale breeding, do not appear in the newspapers at present.
Summary of the invention
In order to shorten the production time of virus-free seed potato, reduce production costs, improve potato seed and quality, the invention provides a kind of seedling-cultivating method of detoxification core seedling being bred in batches convenient strong sprout of transplanting: tissue cultural seedlings of free is cut into several stem sections, after the taking root liquid processing with the agricultural streptomycin that adds the control bacterial disease, cuttage is put into through the tank of sterilization processing and is cultivated in the plastic foam seedling-cultivating tray of particular substrate is housed.Cultivate the shading treatment in early stage, laxative prevention in mid-term late blight, the later stage is drained the water hardening.The method is grown seedlings and can be used in the following aspects: 1, be used for producing breeder's stock in the booth, than the direct planting survival rates height of tissue cultivating seedling, growing way is neat, manageability, unit are production efficiency height.2, offer the direct field planting in land for growing field crops and produce original seed, reduce the link of producing micro potato in the Wembledon tennis open competition chamber, the cost of original seed production link is reduced, and shortened the production time in 1 year.3, scale provides seedling to be used for the mist training and produces breeder's stock, becomes seedling fast, and the seedling gesture is neat,, the convenient transplanting and management.
The technical scheme that the technology of the present invention adopted is:
1, nursery pond is prepared: wide 1.5 meters, dark 0.15 meter bottom sealed cistern is built up in the seedbed in that Wembledon tennis open competition is indoor.With 1% formalin solution clear water is injected in tank sterilization back.
2, matrix is prepared: the perlite and the peat composed of rotten mosses were by 1: 1 proportioning, and every cubic metre of matrix adds 2 kilograms of composite fertilizers of 15: 15: 15 and fully mixes thoroughly.With the matrix spray tide that atomizer spray will be mixed, pack long 0.7 meter, wide 0.4 meter into, the plastic foam seedling-cultivating tray of the bottom of which has holes in dark 0.06 meter 162 caves of every dish.
3, taking root liquid: the naa, the 50PPm agricultural streptomycin that contain 100PPm.
4, detoxic seedling hardening: the detoxic seedling that breeding is come out connects bottle and takes out culturing room, covers 75% sunshade net hardening 3 days in the greenhouse.
5, cuttage: will refine good detoxic seedling and take out from bottle, it is a bit of to be cut into two joints with scissors, puts into sterile shallow bid.Pick up little stem section with sterile tweezers, in taking root liquid, handle the lower end incision of stem section, insert in the hole of matrix in the seedling-cultivating tray.
6, cultivate: stick with a dish back and squirt the cave panel surface, put into nursery pond with sprayer.Hide 75% shading net on the nursery pond, extractd in 5 days after the cuttage.
8, cuttage after 7 days leaf surface spraying concentration be 0.2% special foliage fertilizer once.
10, the water hardening in the nursery pond is drained in cuttage after 10 days.
11, the medicine that sprays the anti-late blight of prevention once.
11, after 12 days-20 days seedling is connected the root carrying substrates from seedling-cultivating tray after the cuttage and take out, for field planting.If field planting ground from grow seedlings far away, the desirable back of emerging connects matrix and wraps with newspaper with one of 50 strain, traverse transports in chest.
The present invention has following advantage with comparing with existing seedling growing process:
1) it is fast to grow seedlings into seedling, and the production time is short, and the whole production time only needs 12-20 days.
2) this method production efficiency height of growing seedlings, a strain detoxification test tube plantlet cuts into the 3-4 section, can after 12 days-20 days, breed can the direct transplanting land for growing field crops strong sprout.
3) only need 10% of common seedling medium consumption with this method substrates quantity, economical with materials is taken greatly.
3) planting percent height can reach more than 95%, and is more much higher than employing cuttage planting percent on the matrix in the seedbed under the similarity condition.
4) convenient management, whole process save a large amount of labours that water and drop into.
5) shoot root that brings out system is flourishing, and matrix forms and is difficult for loose one attached on the root system during taking-up, during transplanting root system be difficult for impaired, the transplant survival height.
6) all can grow seedlings throughout the year, be used for field planting breeding potato seed, need not consider the problem of breaking dormancy.
Examples of implementation 1:
In March, 2007-April, in academy of agricultural sciences, Yunnan Province potato test greenhouse, carry out the operation of matrix method floating seedlings.
1,1 to 15 March is at the group training numerous potato of indoor expansion " cloud potato 301 " virus-elimination seedlings.And will be smooth in original seedbed be tank.March 16, will organize the training detoxic seedling and connect bottle and transport from group training chamber, be put in No. 4 canopies and move hardening on the seedbed, cover 75% shade net.
2, March 18, matrix is prepared: the perlite and the peat composed of rotten mosses add 2 kilograms of composite fertilizer import composite fertilizers of 15: 15: 15 of ratios adding in every cube of matrix and also fully mix thoroughly by 1: 1 proportioning.With the matrix spray tide that sprayer will be mixed, the plastic foam seedling-cultivating tray in 162 holes of every dish of packing into, each cave dish has been prepared 20 dishes altogether with 4 liters of matrix.
3, the same day seedling was taken out in bottle, with scissors be cut into two the joint a bit of, put into sterile shallow bid.Pick up little stem section with sterile tweezers, handle the lower end incision of stem section in taking root liquid, insert in the hole of seedling-cultivating tray, the degree of depth is that lower end joint portion imbeds 1 centimetre of matrix, inserts the matrix at flicking jack place, back, makes matrix be close to cutting stem section.
4, stick with a dish back and squirt the cave panel surface, put into nursery pond with sprayer.3240 strains of growing seedlings are coiled in cuttage 20 altogether.Hide 75% shading net on the nursery pond.
5, extractd shading net behind the sky March 23, after the cuttage March 25 leaf surface spraying concentration be 0.2% foliage fertilizer once.
6, the water of draining in the nursery pond March 28.And with giving birth to 500 times of solution greatly at foliar spray.
7, April 1 seedling is connected the root carrying substrates from seedling-cultivating tray and take out, be used for the plantation of Wembledon tennis open competition chamber and produce breeder's stock.Gathered in the crops breeder's stock on July 5.
The identical time in the comparison of growing seedlings of greenhouse utilization commonsense method, adopt Routine Management method inlet pipe reason.Scale: 8 square metres, cuttage and seedling culture 3200 strains.Investigation: investigation on April 1 planting percent, plant height, blade size, root quantity, root length.Two kinds of transplanting survival rates that method is grown seedlings of investigation on April 8.The quantity and the average simple grain anharmonic ratio of investigation on July 5 results breeder's stock see following table for details.
Examples of implementation 2:
In August, 2008, in academy of agricultural sciences, Yunnan Province potato test greenhouse, carry out the operation of matrix method floating seedlings.
1, preparation: July 23 is to August 10, at the group training numerous potato of indoor expansion " No. one, purple cloud " virus-elimination seedlings.August 15, tank is sterilized with 1% formalin.
2, August 16, will organize the training detoxic seedling and connect bottle from group training chamber taking-up, be put in No. 4 canopies and move hardening on the seedbed, cover 75% shade net.
2,17 to 19 August, matrix is prepared: the perlite and the peat composed of rotten mosses add 2 kilograms of composite fertilizer import composite fertilizers of 15: 15: 15 of ratios adding in every cube of matrix and also fully mix thoroughly by 1: 1 proportioning.With the matrix spray tide that sprayer will be mixed, the plastic foam seedling-cultivating tray in 162 holes of every dish of packing into, each cave dish has been prepared 500 dishes altogether with 4 liters of matrix.Can be for producing quantity 81000 strains of growing seedlings.
3, August 20, seedling is taken out in bottle, with scissors be cut into two the joint a bit of, put into sterile shallow bid.Pick up little stem section with sterile tweezers, handle the lower end incision of stem section in taking root liquid, insert in the hole of seedling-cultivating tray, the degree of depth is that lower end joint portion imbeds matrix more than 1 centimetre, inserts the matrix at flicking jack place, back, makes matrix be close to cutting stem section.
4, stick with a dish back and squirt the cave panel surface, put into nursery pond with sprayer.Hide 75% shading net on the nursery pond.Cuttage always is to end of day in Augusts 26, cuttage every day put into a nursery pond, 81000 strains of growing seedlings altogether.
5, extract shading net gradually after August 25, every day on August 27 to September 1 respectively to the cuttage seeding leaf surface spraying concentration of different time be 0.2% foliage fertilizer once.
6, the water of draining in the nursery pond August 30.And with giving birth to 500 times of liquid greatly at foliar spray.
7, beginning on September 2 connects the root carrying substrates with seedling from seedling-cultivating tray and takes out, and transplants to No. 2 Wembledon tennis open competition chamber plantation production breeder's stocks.December 10 gathered in the crops, classification, counting, packing.
In identical, directly in No. 1 booth seedbed, adopt the cuttage of normal cutting propagation method 50,000 seedlings produce breeder's stock.
Investigation: investigation on September 2 planting percent, plant height, blade size, root quantity, root length.Investigation on September 15 transplanting survival rate, December 15, quantity, individual plant knot potato number, the average simple grain of statistics breeder's stock were heavy.Relatively see following table for details.
Figure BSA00000406292400051
Examples of implementation 3:
In March, 2009, in academy of agricultural sciences, Yunnan Province potato test greenhouse, carry out the operation of matrix method floating seedlings, original seed is bred in transplanting to big wheat field village, Kunming Dian Yuan town plantation.
1, preparation: March 15 to 23, at the group training numerous potato of indoor expansion " cloud potato 301 " virus-elimination seedlings.
2, March 25, will organize the training detoxic seedling and connect bottle and transport from group training chamber, be put in No. 4 canopies and move hardening on the seedbed, cover 75% sunshade net.
3, March 28, nursery pond is sterilized with 1% formalin.
4, matrix is prepared: the perlite and the peat composed of rotten mosses add 2 kilograms of import composite fertilizers of 15: 15: 15 by every cubic metre of matrix and also fully mix thoroughly by 1: 1 proportioning.With the matrix spray tide that sprayer will be mixed, the plastic foam seedling-cultivating tray in 162 holes of every dish of packing into, each cave dish has been prepared 40 dishes altogether with 4 liters of matrix.Can be for producing quantity 6480 strains of growing seedlings.
5, March 28, seedling is taken out in bottle, with scissors be cut into two the joint a bit of, put into sterile shallow bid.Pick up little stem section with sterile tweezers, handle the lower end incision of stem section in taking root liquid, insert in the hole of seedling-cultivating tray, the degree of depth is that lower end joint portion imbeds matrix more than 1 centimetre, inserts the matrix at flicking jack place, back, makes matrix be close to cutting stem section.
6, stick with a dish back and squirt the cave panel surface, put into nursery pond with sprayer.Hide 75% shading net on the nursery pond.
7, extract shading net on April 5, sprayed concentration on April 8 and be 0.2% foliage fertilizer once.
8, hardening: drained the water in the nursery pond on April 14.
9, gave birth to 500 times of liquid at foliar spray with big at dusk the same day.
9, connect the root carrying substrates with seedling from seedling-cultivating tray and take out April 16, and one of 50 strain are wrapped with newspaper, are loaded on carton, are transported to big wheat field village, Dian Yuan town, Kunming.Field planting in the morning on April 17.Gathered in the crops original seed on September 3.
Investigation: investigation cuttage on April 16 planting percent, plant height, blade size, root quantity, root length.Investigation on May 6 field-transplanting survival rate, September 3, the statistics individual plant was tied potato amount and per mu yield.
Investigation sees following table for details.
Figure BSA00000406292400071
Breeding original seed with the plantation breeder's stock compares:
Figure BSA00000406292400072
Annotate: original seed is produced 700 yuan/mu of other cost meters.

Claims (6)

1. a potato virus-free plantlet stem segment base matter floating seedlings culturing method is characterized in that comprising the steps:
1), wide 1.5 meters, dark 0.15 meter bottom sealed cistern is built up in the seedbed in that Wembledon tennis open competition is indoor.With 1% formalin solution clear water is injected in tank sterilization back.
2), matrix is prepared: the perlite and the peat composed of rotten mosses were by 1: 1 proportioning, and every cubic metre of matrix adds 2kg composite fertilizer (N: P: K is 15: 15: 15) and fully mixes thoroughly.With the matrix spray tide that atomizer spray will be mixed, pack long 0.7 meter, wide 0.4 meter into, the plastic foam seedling-cultivating tray of the bottom of which has holes in dark 0.06 meter 162 caves of every dish.
3), handle the taking root liquid of stem section: the naa that contains 100PPm adds the 50PPm agricultural streptomycin.
4), detoxic seedling hardening: the detoxic seedling that breeding is come out connects bottle and takes out culturing room, covers 75% sunshade net hardening 3 days in the greenhouse.
5), cuttage: will refine good detoxic seedling and take out from bottle, it is a bit of to be cut into two joints with scissors, puts into sterile shallow bid.Pick up little stem section with sterile tweezers, in taking root liquid, handle the lower end incision of stem section, insert in the hole of seedling-cultivating tray.
6), stick with a dish back and squirt the cave panel surface with sprayer, put into nursery pond.Hide 75% shading net on the nursery pond, extractd in 5 days after the cuttage.
7), cuttage after 7 days leaf surface spraying concentration be 0.2% special foliage fertilizer once.Drain the water hardening in the nursery pond after cuttage 10-12 days, the medicine that sprays the anti-late blight of prevention once.After 12 days-20 days seedling is connected the root carrying substrates from seedling-cultivating tray after the cuttage and take out, for field planting.
2. the method for potato virus-free plantlet stem segment base matter method floating seedlings according to claim 1 is characterized in that: 1.5 meters, dark 0.15 meter bottom sealed cistern are built up in the seedbed, and it is stand-by with 1% formalin solution clear water to be injected in tank sterilization back.
3. the method for potato virus-free plantlet stem segment base matter method floating seedlings according to claim 1, it is characterized in that: matrix is pressed perlite and 1: 1 proportioning of the peat composed of rotten mosses, 2 kilograms of N: P: K of every cube of matrix adding are that 15: 15: 15 composite fertilizer fully mixes thoroughly, with the plastic foam seedling-cultivating tray of the bottom of which has holes of packing into behind the sprayer spray tide.
4. the method for potato virus-free plantlet stem segment base matter method floating seedlings according to claim 1 is characterized in that: the taking root liquid of handling the stem section is that the naa of 100PPm adds the 50PPm agricultural streptomycin.
5. the method for potato virus-free plantlet stem segment base matter method floating seedlings according to claim 1, it is characterized in that: tissue cultural seedlings of free is cut into stem section of two joints with scissors, in taking root liquid, handle the lower end incision of stem section, insert in the matrix in seedling-cultivating tray hole.
6. the method for potato virus-free plantlet stem segment base matter method floating seedlings according to claim 1 is characterized in that: cultivate preceding 5 days with 75% shading net shading, cuttage sprayed foliage fertilizer once after 7 days; The water hardening in the nursery pond is drained in cuttage after 10 days, spray the prevention late blight medicine once.
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Cited By (11)

* Cited by examiner, † Cited by third party
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CN102657081A (en) * 2012-04-28 2012-09-12 四川省农业科学院作物研究所 Method for culturing potato virus-free test-tube plantlets in water culture mode
CN103039245A (en) * 2013-01-05 2013-04-17 凉山州西昌农业科学研究所 Potato seed facility seedling raising method
CN103348864A (en) * 2013-07-22 2013-10-16 信阳农林学院 Method for cutting propagation of gardenias through plugging shallow water bathing
CN104303748A (en) * 2014-09-28 2015-01-28 王慧 Geranium facility cultivation rapid propagation method
CN104429866A (en) * 2014-12-24 2015-03-25 延边大学 Method for cultivating small potatoes which germinate without dormancy
CN105123488A (en) * 2015-08-28 2015-12-09 肖玉兰 Plant tissue culture rooting and seedling exercising integrated cultivating device and cultivating method thereof
CN106747817A (en) * 2016-12-29 2017-05-31 广西壮族自治区亚热带作物研究所 Sisal hemp floating seedlings nutrient solution and sisal hemp float seedling method
CN107624583A (en) * 2017-10-20 2018-01-26 山西省农业科学院玉米研究所 The method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area
CN110583141A (en) * 2019-09-09 2019-12-20 玉溪市农业科学院 Potato seed floating seedling transplantation method
CN110692471A (en) * 2019-11-07 2020-01-17 云南省农业科学院经济作物研究所 Cultivation management method for seed production of early-spring potato seeds
CN110692472A (en) * 2019-11-07 2020-01-17 云南省农业科学院经济作物研究所 Method for field transplanting and breeding stock seeds of potato low latitude plateau tissue culture seedlings

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102657081A (en) * 2012-04-28 2012-09-12 四川省农业科学院作物研究所 Method for culturing potato virus-free test-tube plantlets in water culture mode
CN102657081B (en) * 2012-04-28 2014-09-17 四川省农业科学院作物研究所 Method for culturing potato virus-free test-tube plantlets in water culture mode
CN103039245A (en) * 2013-01-05 2013-04-17 凉山州西昌农业科学研究所 Potato seed facility seedling raising method
CN103039245B (en) * 2013-01-05 2014-06-04 凉山州西昌农业科学研究所 Potato seed facility seedling raising method
CN103348864A (en) * 2013-07-22 2013-10-16 信阳农林学院 Method for cutting propagation of gardenias through plugging shallow water bathing
CN103348864B (en) * 2013-07-22 2014-12-10 信阳农林学院 Method for cutting propagation of gardenias through plugging shallow water bathing
CN104303748A (en) * 2014-09-28 2015-01-28 王慧 Geranium facility cultivation rapid propagation method
CN104429866A (en) * 2014-12-24 2015-03-25 延边大学 Method for cultivating small potatoes which germinate without dormancy
CN105123488A (en) * 2015-08-28 2015-12-09 肖玉兰 Plant tissue culture rooting and seedling exercising integrated cultivating device and cultivating method thereof
CN105123488B (en) * 2015-08-28 2018-05-15 上海离草科技有限公司 Plant tissue culture is taken root, the integrated culture apparatus of hardening and its cultural method
CN106747817A (en) * 2016-12-29 2017-05-31 广西壮族自治区亚热带作物研究所 Sisal hemp floating seedlings nutrient solution and sisal hemp float seedling method
CN107624583A (en) * 2017-10-20 2018-01-26 山西省农业科学院玉米研究所 The method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area
CN110583141A (en) * 2019-09-09 2019-12-20 玉溪市农业科学院 Potato seed floating seedling transplantation method
CN110692471A (en) * 2019-11-07 2020-01-17 云南省农业科学院经济作物研究所 Cultivation management method for seed production of early-spring potato seeds
CN110692472A (en) * 2019-11-07 2020-01-17 云南省农业科学院经济作物研究所 Method for field transplanting and breeding stock seeds of potato low latitude plateau tissue culture seedlings
CN110692471B (en) * 2019-11-07 2021-08-20 云南省农业科学院经济作物研究所 Cultivation management method for seed production of early-spring potato seeds

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Application publication date: 20110810