CN101707979A - Sugarcane tissue-culture-seedling bare-seedling field-transplanting method - Google Patents
Sugarcane tissue-culture-seedling bare-seedling field-transplanting method Download PDFInfo
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- CN101707979A CN101707979A CN200910114601A CN200910114601A CN101707979A CN 101707979 A CN101707979 A CN 101707979A CN 200910114601 A CN200910114601 A CN 200910114601A CN 200910114601 A CN200910114601 A CN 200910114601A CN 101707979 A CN101707979 A CN 101707979A
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Abstract
The invention provides a sugarcane tissue-culture-seedling bare-seedling field-transplanting method which comprises the following steps: culturing sugarcane tissue culture seedlings in a sound seedling culture medium for 1-2 generations; carrying out the rooting culture; group-planting in a seedling breeding garden to breed the seedlings for 30-40 days; after each sugarcane seedling has the independent root system, hardening the group-planted seedlings, dividing the seedlings, and directly transplanting each bare individual seedling to a prepared seedling breeding field; and after planting, carrying out the management for enhancing the survival percent, and the like. Compared with the conventional tissue culture seedling breeding transplanting method, the method of the invention reduces the step of tissue-culture-seedling nutritive cup temporary planting, can effectively solve the problems of long nursery breeding time before sugarcane tissue culture seedling is planted to the field, large occupation area in the nursery, high production cost and the like, and has the advantages of easy seedling lifting, convenient transportation and transportation cost saving. The invention prolongs the field growth time and enhances the per unit yield by about 25%.
Description
Technical field
The present invention relates to the propagation production of cane seedling, specifically belong to a kind of method of sugarcane tissue-culture-seedling bare-seedling field-transplanting.
Background technology
In order to quicken the new good popularization reproduction speed that reaches the detoxification health seedling of sugarcane, adopt advanced plant tissue culture technique cane seedling to be carried out numerous soon.At present the sugarcane tissue-culture seedling at bottle seedling of taking root to transplanting the land for growing field crops during this period of time, generally plant, divide links such as the individual plant nutrient cup is heeled in, field-transplanting through the husky bed clump in nursery, breeding with popularization of seedling there is big adverse effect, main performance has: (1) individual plant nutrient cup is heeled in and is grown seedlings that to take the nursery time long, efficient was hanged down in conditions such as illumination, temperature and moisture 4～October preferably, nutrient cup is heeled in seedling and is heeled in to going out garden 30～40 day time of need from plant division, it is bigger to heel in the shared area of nursery bed of nutrient cup seedling, is about 4 times that clump is planted the seedling area; (2) the high nutrient cup seed of seedling cost is planted, nursery bed management, goes out work such as garden, need to drop into a large amount of material resources, labour, and transportation is inconvenient, the production cost height, and the comprehensive average seedling cost of every young plant reaches about 0.2 yuan; (3) because tissue cultivating seedling is long in the time in nursery, postpone to transplant, shortened the grown in field time relatively, output reduces, and is unfavorable for the numerous and promotion rate of breeding tissue culture sprout quick.
Summary of the invention
The method that the purpose of this invention is to provide a kind of sugarcane tissue-culture-seedling bare-seedling field-transplanting.Reduce the tissue cultivating seedling nutrient cup and heel in this link, can solve the sugarcane tissue-culture seed effectively and plant land for growing field crops preceding nursery cultivation time length, take the problem that the site area of growing seedlings is big, production cost is high, improve tissue cultivating seedling land for growing field crops unit are kind stem output.
The present invention is achieved through the following technical solutions:
The sugarcane tissue-culture seedling was cultivated for 1～2 generation in the strong seedling culture base earlier, carry out culture of rootage then, the metaplexus that takes root is planted in and grows seedlings 30～40 days in the Seedlings nursery tissue cultivating seedling, when treating that every strain sugarcane seedling all has independently root system, clump is planted seedling carry out plant division after the suitable hardening, naked then seedling individual plant direct transplanting is to ready seedling breeding land for growing field crops.
Described strong seedling culture base is the MS minimal medium that does not contain hormone.
In 1～2 generation of described cultivation, be incubation time, about 15～20 days of 1 generation.
Described culture of rootage is to take root in the medium of tissue cultivating seedling at MS+NAA5mg/L of strong seedling culture.
Described clump is planted, and is bottle seedling of taking root is carried out the hardening cultivation after 3～7 days outdoor, and bottle seedling of will taking root shifts out outside the bottle, and every bottle of seedling is divided into some clumps, and every Cong Miao 3～5 strain seedlings are planted on the seedbed then.
Described Seedlings nursery is to have the windproof printing opacity of waterproof seedling growth greenhouse preferably, and seedling medium is mainly aseptic new soil or river sand.
Describedly clump is planted seedling carrying out suitable hardening, is about the false stem of sugarcane seedling is up to 15 centimetres, stops fertilising, and reduces using of water gradually, after 5～6 days, stops trickle 2～3 days, treats that the sugarcane seedling carries out plant division when occurring slightly suffering from drought symptom.
Described plant division is to pull up from the seedbed planting seedling through the clump of hardening, then every young plant is separated separately, prepares to transplant the land for growing field crops.
Described naked seedling direct transplantation is with above-mentioned 7 individual plant seedling, cuts off 2/3 blade, with containing ABT3 root-inducing powder yellow mud slurry root, is colonizated in the land for growing field crops by certain planting density then, drenches sufficient normal root water.
The survival rate management is improved in the described back that plants, and is group training root-naked transplanting, after all left and right sides sugarcane seedling survives after the field planting, enters normal field management work.
The present invention has reduced the tissue cultivating seedling nutrient cup and has heeled in this link, can solve the sugarcane tissue-culture seed effectively and plant land for growing field crops preceding nursery cultivation time length, take the problem that the site area of growing seedlings is big, production cost is high, and lifting is easy, and convenient transportation is saved cost of transportation; Prolong the grown in field time, increase the yield per unit area about 25%.Per hectare can volume increase be worth 6400 yuan, remarkable in economical benefits.
Embodiment
The present invention is described in further detail by following specific embodiment, but the present invention trains the seedling bare-seedling field-transplanting time and content is not limited thereto.
1. cultivate the sturdy seedling of taking root
With new platform sugar is donor No. 22, carries out stem apex detoxify 7～August and handles, and breeds tissue cultural seedlings of free, and proliferated culture medium is MS+6-BA1.0-1.5mg/L+NAA0.1mg/L, breeds under natural scattering light, 28 ℃ of conditions of temperature.When tissue cultivating seedling is bred to 5～6 generations, change it in the MS medium that does not contain hormone about 30 days (1～2 time in generation) of cultivation, reduce growth rate, make medium nutrition satisfy existing tissue cultivating seedling stalwartness to greatest extent and grow.Tissue cultivating seedling through strong seedling culture was taken root under MS+NAA 5mg/L condition the late January in 1 year, grew root system in about about 20 days, and its rooting rate and root system quality are all higher.
MS is that the most general a kind of medium title is used in Plant Tissue Breeding, and main component is for planting the required number of chemical material of growth, its public use of filling a prescription; The 6-BA Chinese is a 6-benzyl aminoadenine, and the NAA Chinese is a naa, and sowing rich garden supplies Co., Ltd by Shanghai provides.
2. cultivate healthy and strong transplanted seedling
After tissue cultivating seedling is taken root, the bottle seedling is shifted out outdoor (late Febuary), be placed on rainproof, hide and to sprout, to loose and thank in the booth of light intensity, ventilation, carrying out hardening cultivated after 10 days, bottle seedling of will taking root shifts out outside the bottle, and every bottle of seedling is divided into some clumps, every Cong Miao 3～5 strain seedlings, clean up, cut off the long blade of part, be soaked in 0.1%～0.2% the liquor potassic permanganate 1～2 minute, clump is planted on the husky bed of protective equipment then, 8～10 centimetres of line-spacings, clump is apart from 6～8 centimetres; Clump is planted after seedling survives, and drenches 2～3 times with the ternary import composite fertilizer concentration about 0.10% of effective ingredient 45% weekly, cuts 1～2 leaf strong sprout weekly, urgees root; Under the normal condition of weather condition, the false stem of early April sugarcane seedling is high about 15 centimetres, stops fertilising, and reduces using of water gradually, after 5～6 days, stops trickle 2～3 days, treats to transplant when the sugarcane seedling occurs slightly suffering from drought symptom.
3. prepare in the transplanted seedling field
The seedling breeding land for growing field crops is the soil of preparing the plantation tissue cultivating seedling, selects that fertile soil, irrigation and drainage are convenient, sun-drenched plot is the tissue cultivating seedling land used of planting.Meticulous whole ground is carried out in the sugarcane field, require to reach dark, loose, broken, flat wholely, hold the plantation ditch then successfully, 1.0～1.2 meters of line-spacings, about 35 centimetres of ditch depths; About tissue cultivating seedling is transplanted preceding 1 week, use 50 kilograms of the ternary compound fertilizers of content 25% planting every mu in ditch, and mix about 10 centimetres of blindings thoroughly with soil; , convert water 30～50kg according to the soil moisture situation and evenly spray ground with 38% atrazine 200ml+50% Acetochlor 100ml at plantation ditch mu; Behind the herbicide spraying, covering with plastic film then.
4. tissue-culture-seedling bare-seedling field-transplanting
Early April, the sugarcane seedling after hardening transplanted mid-April.The clump of prepare transplanting planted carry out the branch individual plant after seedling pulls up, classification by size cuts off 2/3 blade with every blade of every young plant, with contain ABT3 root-inducing powder (Chinese forest-science academy ABT research and development centre provides), yellow mud is transplanted the land for growing field crops after starching root; Punch by planting density with diameter 5cm left and right sides wooden stick earlier before transplanting, the dark 10～12cm in hole, a seedling is put in a hole, and system vertically puts into the hole earthing with the sugarcane shoot root, and the degree of depth is grasped to cover about root system 1cm, drenches sufficient normal root water then.
5. plant the back and improve the survival rate management
Behind the group training root-naked transplanting, the weather condition during according to plantation is carried out moisture and sunshade management, to improve survival rate.It is bigger that this moment plantation, daytime temperature are no more than 30 ℃, soil moisture, before surviving every day trickle 1 time; If temperature height, the sun are fierce, every day is trickle 1 time sooner or later, and carries out sunshade.About one all left and right sides sugarcane seedling survives after the field planting, can enter normal field management work.
6. implementation result
Group training clump is planted seedling and divides individual plant to plant in late April.Train naked seedling direct transplantation land for growing field crops for of the present invention group, can cut to late August and take in numerous kind of capable fall planting.As seen from Table 1, the naked seedling direct transplantation of group training land for growing field crops, because it does sth. in advance the field planting time, its plant height, stem footpath, unit are productive tiller, output etc. all are better than contrast (nutrient cup is heeled in seedling), remarkable in economical benefits.
By the existing sugarcane area in Guangxi, the annual cultivation sugarcane tissue-culture seedling one-level seedling area that needs is counted more than 1000 hectares:
(1) saves seedling cost
Use healthy tissue-cultured seedling 30000 strains of sugarcane detoxication in per hectare, reduce and divide individual plant to heel in this link, heel in 0.2 yuan of glass seedling production and transport cost by every strain, then per hectare is saved 6000 yuan cost expenditure, the 1000 hectares of cost expenditures that then can save 6,000,000 yuan;
(2) increase the unit are income
By 500 yuan on per ton kind of stem, per hectare can the volume increase value=(62.7-49.9) * and 500=6400 unit, then can increase income 6,400,000 yuan for 1000 hectares.
Table 1 group is trained naked seedling direct transplantation grown in field information slip
Claims (9)
1. the method for a sugarcane tissue-culture-seedling bare-seedling field-transplanting, it is characterized in that: the sugarcane tissue-culture seedling carries out culture of rootage after cultivating for 1～2 generation earlier in the strong seedling culture base, the metaplexus that takes root is planted in and grows seedlings 30～40 days in the Seedlings nursery tissue cultivating seedling, when treating that every strain sugarcane seedling all has independently root system, clump is planted seedling carry out plant division after the suitable hardening, naked then seedling individual plant direct transplanting is to ready seedling breeding land for growing field crops.
2. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1, it is characterized in that: described strong seedling culture base is the MS minimal medium that does not contain hormone.
3. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1 is characterized in that: in 1～2 generation of described cultivation, be incubation time, about 15～20 days of 1 time in generation.
4. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1, it is characterized in that: described clump is planted, be that bottle seedling of will taking root carries out after hardening cultivates 3～7 days outdoor, bottle seedling of will taking root shifts out outside the bottle, every bottle of seedling is divided into some clumps, every Cong Miao 3～5 strain seedlings are planted on the seedbed then.
5. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1, it is characterized in that: described Seedlings nursery is to have the windproof printing opacity of waterproof seedling growth greenhouse preferably, and seedling medium is mainly aseptic new soil or river sand.
6. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1, it is characterized in that: describedly clump is planted seedling carry out suitable hardening, be about the false stem of sugarcane seedling is up to 15 centimetres, stop fertilising, and reduce using of water gradually, after 5～6 days, stopped trickle 2～3 days, treat that the sugarcane seedling carries out plant division when occurring slightly suffering from drought symptom.
7. according to the method for claim 1 or 6 described sugarcane tissue-culture-seedling bare-seedling field-transplantings, it is characterized in that: described plant division is to pull up from the seedbed planting seedling through the clump of hardening, then every young plant is separated separately, prepares to transplant the land for growing field crops.
8. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1, it is characterized in that: described naked seedling direct transplantation, be with above-mentioned 7 individual plant seedling, cut off 2/3 blade, with containing ABT3 root-inducing powder yellow mud slurry root, be colonizated in the land for growing field crops by certain planting density then, drench sufficient normal root water.
9. the method for sugarcane tissue-culture-seedling bare-seedling field-transplanting according to claim 1 is characterized in that: the survival rate management is improved in the described back that plants, and is group training root-naked transplanting, after all left and right sides sugarcane seedling survives after the field planting, enters normal field management work.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102405837A (en) * | 2011-09-06 | 2012-04-11 | 广西亚热带作物研究所试验站 | Method for transplanting sugarcane tissue culture seedlings to fields from low tunnels |
| CN102498912A (en) * | 2011-11-23 | 2012-06-20 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
| CN104381107A (en) * | 2014-11-06 | 2015-03-04 | 海南省农业科学院热带果树研究所 | Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling |
| CN104770173A (en) * | 2015-04-13 | 2015-07-15 | 云南省农业科学院甘蔗研究所 | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings |
| CN104823848A (en) * | 2015-05-05 | 2015-08-12 | 广州甘蔗糖业研究所 | Root-inducing strong seedling medium for sugarcane and direct field planting method of sugarcane tissue culture bottle seedling |
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| CN106614624A (en) * | 2016-11-02 | 2017-05-10 | 广西壮族自治区农业科学院甘蔗研究所（中国农业科学院甘蔗研究中心） | Herbicide for seedling of sugar cane and using method thereof |
| CN106888903A (en) * | 2017-02-08 | 2017-06-27 | 合肥师范学院 | A kind of blueberry tissue culture bare-root seeding field-transplanting method |
| CN107736217A (en) * | 2017-11-01 | 2018-02-27 | 李福元 | A kind of field production method of sugarcane detoxication tissue culturing seedling |
| CN107980531A (en) * | 2017-12-25 | 2018-05-04 | 洛阳名力科技开发有限公司 | A kind of method for transplanting of sugarcane tissue-culture nursery |
| CN110506600A (en) * | 2019-08-28 | 2019-11-29 | 广西壮族自治区农业科学院甘蔗研究所 | Utilize the method for sugarcane health seedling ineffective tillering nursery |
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| CN111642350A (en) * | 2020-06-30 | 2020-09-11 | 广西壮族自治区农业科学院 | Cultivation method of sugarcane |
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| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
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| CN101361458B (en) * | 2008-09-27 | 2011-09-28 | 云南省农业科学院甘蔗研究所 | Efficient breeding method of sugarcane health seedling |
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- 2009-11-30 CN CN2009101146014A patent/CN101707979B/en not_active Expired - Fee Related
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| CN102405837A (en) * | 2011-09-06 | 2012-04-11 | 广西亚热带作物研究所试验站 | Method for transplanting sugarcane tissue culture seedlings to fields from low tunnels |
| CN102498912A (en) * | 2011-11-23 | 2012-06-20 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
| CN102498912B (en) * | 2011-11-23 | 2014-07-23 | 云南省农业科学院甘蔗研究所 | Method for transplanting sugar-cane tissue culture seedlings |
| CN104381107A (en) * | 2014-11-06 | 2015-03-04 | 海南省农业科学院热带果树研究所 | Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling |
| CN104770173A (en) * | 2015-04-13 | 2015-07-15 | 云南省农业科学院甘蔗研究所 | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings |
| CN104823848A (en) * | 2015-05-05 | 2015-08-12 | 广州甘蔗糖业研究所 | Root-inducing strong seedling medium for sugarcane and direct field planting method of sugarcane tissue culture bottle seedling |
| CN105165548A (en) * | 2015-09-01 | 2015-12-23 | 福建省春天园林工程有限公司 | Megaphanerophyte group planting cultivation method |
| CN106614624A (en) * | 2016-11-02 | 2017-05-10 | 广西壮族自治区农业科学院甘蔗研究所（中国农业科学院甘蔗研究中心） | Herbicide for seedling of sugar cane and using method thereof |
| CN106888903A (en) * | 2017-02-08 | 2017-06-27 | 合肥师范学院 | A kind of blueberry tissue culture bare-root seeding field-transplanting method |
| CN107736217A (en) * | 2017-11-01 | 2018-02-27 | 李福元 | A kind of field production method of sugarcane detoxication tissue culturing seedling |
| CN107980531A (en) * | 2017-12-25 | 2018-05-04 | 洛阳名力科技开发有限公司 | A kind of method for transplanting of sugarcane tissue-culture nursery |
| CN110506600A (en) * | 2019-08-28 | 2019-11-29 | 广西壮族自治区农业科学院甘蔗研究所 | Utilize the method for sugarcane health seedling ineffective tillering nursery |
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| CN111642350A (en) * | 2020-06-30 | 2020-09-11 | 广西壮族自治区农业科学院 | Cultivation method of sugarcane |
| CN111727687A (en) * | 2020-06-30 | 2020-10-02 | 广西壮族自治区农业科学院 | Cultivation method of sugarcane transplanted seedlings |
| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
| CN113545268B (en) * | 2021-08-13 | 2022-09-09 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
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