CN107980531A - A kind of method for transplanting of sugarcane tissue-culture nursery - Google Patents

A kind of method for transplanting of sugarcane tissue-culture nursery Download PDF

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Publication number
CN107980531A
CN107980531A CN201711421940.8A CN201711421940A CN107980531A CN 107980531 A CN107980531 A CN 107980531A CN 201711421940 A CN201711421940 A CN 201711421940A CN 107980531 A CN107980531 A CN 107980531A
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CN
China
Prior art keywords
transplanting
days
culture
sugarcane
seedling
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Pending
Application number
CN201711421940.8A
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Chinese (zh)
Inventor
李蓓
孙琼珂
周军州
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Luoyang Mingli Science and Technology Development Co Ltd
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Luoyang Mingli Science and Technology Development Co Ltd
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Publication date
Application filed by Luoyang Mingli Science and Technology Development Co Ltd filed Critical Luoyang Mingli Science and Technology Development Co Ltd
Priority to CN201711421940.8A priority Critical patent/CN107980531A/en
Publication of CN107980531A publication Critical patent/CN107980531A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants

Abstract

A kind of method for transplanting of sugarcane tissue-culture nursery, sugarcane tissue-cultured seedling bottle seedling directly carries out culture of rootage in culturing room after Multiplying culture, take out and clean up from bottle after rooting, soaked with carbendazim and wait to plant, heeled in Seedlings nursery and carry out point single plant and transplant to the seedling breeding crop field made preparation for ploughing and sowing;First plant sugarcane ditch is drenched with water before transplanting and is drenched, transplanting is finished builds Small plastic shed above plant sugarcane ditch, highly the film being dimensioned for is selected to be covered on Small plastic shed according to line-spacing and Small plastic shed, the top both sides that film is placed on seedling land, and both ends open and stay ventilating opening, screened negative is placed on outside film and by the ventilator hole cover of both ends, the transplanting same day opens an air hole at the top of Small plastic shed, after 3~5 days, expands air hole, transplanting raised film after 8 days completely, and watering in two days is once;And first time fertilizer is applied based on composite fertilizer, once in a week, sugarcane tissue-culture transplantation of seedlings transplants crop field in more than 40 days.Simple to operate, cost is low, and sugar-cane tissue culture seedlings transplanting survival rate is high.

Description

A kind of method for transplanting of sugarcane tissue-culture nursery
Technical field
The invention belongs to plant fast propagation production technology, is specifically a kind of method for transplanting of sugarcane tissue-culture nursery.
Background technology
Sugarcane tissue culture is a kind of efficient reproduction technique, can accelerate the quick breeding of sugarcane improved seeds, anniversary The sugarcane improved seeds tissue-cultured seedling of large-scale production character neat and consistent supplies production application, so that it is short to solve each sugarcane district breeding seedling Lack, improved seeds promote the problems such as slow and cost of transportation is high.
Sugarcane (Saccharum.ssp) is the most important sugar crop in China, and most important energy-source plant in the world. The development of sugarcane science and technology have become Sugarcane Industry development new power, but science and technology practicality and replicability Decide the practical application of technology again.Sugarcane is a kind of crop of vegetative propagation, in production mostly or using propagation of seed stock as It is main.Although the tissue culture technology of sugarcane is ripe already, using long-term deficiency in production, in many sugarcane districts because production technology falls behind also Fail the popularizing planting sugar-cane tissue culture seedlings of large area, therefore, in terms of the popularization and application of sugarcane health seedling and new variety of sugarcane Tissue-cultured seedling is not applied fully also.Sugarcane tissue culture technology also needs to further develop, particularly in tissue culture transplantation of seedlings skill In terms of art.The transplanting survival rate of sugar-cane tissue culture seedlings largely affects the quality of tissue-cultured seedling and the efficiency of tissue cultures, Meanwhile and tissue-cultured seedling be applied to the key technology of each sugarcane district.A kind of simple, efficient, quick sugarcane tissue-culture transplantation of seedlings Technology be at present we there is an urgent need to.
The content of the invention
It is an object of the invention in view of the above-mentioned problems, proposing a kind of method for transplanting of sugarcane tissue-culture nursery.
To achieve these goals, the present invention is realized using following technical scheme.It is sweet according to one kind proposed by the present invention The method for transplanting of sugarcane tissue culture, sugarcane tissue-cultured seedling bottle seedling are directly given birth in culturing room after the Multiplying culture in 2~4 generations Root culture, takes out after rooting from bottle and cleans up, and waits to plant with 0.5% carbendazim immersion 10min;
It is divided into 3~8 plants/clump and is heeled in Seedlings nursery and carries out within 15~20 days point single plant and transplant to the seedling breeding made preparation for ploughing and sowing Crop field;First plant sugarcane ditch is drenched with water before transplanting and is drenched, transplanting finishes and builds Small plastic shed above sugarcane ditch planting, according to line-spacing and small Shed highly selects the film being dimensioned for be covered on Small plastic shed, and the top both sides that film is placed on seedling land, and both ends are opened and stayed Ventilating opening, screened negative is placed on outside film and by the ventilator hole cover of both ends, the transplanting same day opens one at the top of Small plastic shed every 30~60 ㎝ A 2 ㎝ air holes of diameter;
After transplanting 3~5 days, expand air hole to 10 ㎝ of diameter, transplanting and raise film completely after 8 days, watering one in two days It is secondary;And apply based on the composite fertilizer of fertilizer 0.05% for the first time, once in a week;Cutting seedlings is once weekly;0.15% nitrogenous fertilizer is affixed by after 20 days Or composite fertilizer;Sugarcane tissue-culture transplantation of seedlings transplants crop field in more than 40 days.
A kind of method for transplanting of sugarcane tissue-culture nursery proposed by the present invention is in outdoor transplanting, rather than is moved in greenhouse Plant, realize that tissue culture is once transplanted, transplanting process is short, uses manpower and material resources sparingly, and efficiently and survival rate is high;It is simple to operate, Cost is low, and sugar-cane tissue culture seedlings transplanting survival rate is adapted to the needs of industrial massive production up to 90% -95%.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention, and It can be practiced according to the content of specification, and to allow the above and other objects, features and advantages of the present invention can be brighter It is aobvious understandable, described in detail below especially exemplified by preferred embodiment.
Embodiment
The present invention is described in further detail below by specific embodiment.
A kind of method for transplanting of sugarcane tissue-culture nursery, sugarcane tissue-cultured seedling bottle seedling are trained in culturing room by the propagation in 2~4 generations Culture of rootage is directly carried out after supporting, takes out and cleans up from bottle after rooting, is treated with 0.5% carbendazim immersion 10min Kind;
It is divided into 3~8 plants/clump and is heeled in Seedlings nursery and carries out within 15~20 days point single plant and transplant to the seedling breeding made preparation for ploughing and sowing Crop field;First plant sugarcane ditch is drenched with water before transplanting and is drenched, transplanting finishes and builds Small plastic shed above sugarcane ditch planting, according to line-spacing and small Shed highly selects the film being dimensioned for be covered on Small plastic shed, and the top both sides that film is placed on seedling land, and both ends are opened and stayed Ventilating opening, screened negative is placed on outside film and by the ventilator hole cover of both ends, the transplanting same day opens one at the top of Small plastic shed every 30~60 ㎝ A 2 ㎝ air holes of diameter;
After transplanting 3~5 days, expand air hole to 10 ㎝ of diameter, transplanting and raise film completely after 8 days, watering one in two days It is secondary;And apply based on the composite fertilizer of fertilizer 0.05% for the first time, once in a week;Cutting seedlings is once weekly;0.15% nitrogenous fertilizer is affixed by after 20 days Or composite fertilizer;Sugarcane tissue-culture transplantation of seedlings transplants crop field in more than 40 days.
A kind of method for transplanting of sugarcane tissue-culture nursery proposed by the present invention is in outdoor transplanting, rather than is moved in greenhouse Plant, realize that tissue culture is once transplanted, transplanting process is short, uses manpower and material resources sparingly, and efficiently and survival rate is high;It is simple to operate, Cost is low, and sugar-cane tissue culture seedlings transplanting survival rate is adapted to the needs of industrial massive production up to 90% -95%.
Above in association with the specific embodiment technical principle that the invention has been described.These descriptions are intended merely to explain the present invention's Principle, and limiting the scope of the invention cannot be construed in any way.Base of the those skilled in the art in the present invention Various modifications or additions are done on plinth or are substituted in a similar way, without departing from structure of the invention or are surmounted Scope defined in the claims, should all belong to the protection category of the present invention.

Claims (1)

  1. A kind of 1. method for transplanting of sugarcane tissue-culture nursery, it is characterised in that:Sugarcane tissue-cultured seedling bottle seedling passed through for 2~4 generations in culturing room Multiplying culture after directly carry out culture of rootage, takes out after rooting and cleans up from bottle, with 0.5% carbendazim immersion 10min waits to plant, be divided into 3~8 plants/clump heeled in Seedlings nursery carry out within 15~20 days point single plant transplant it is numerous to the seedling made preparation for ploughing and sowing Educate crop field;First plant sugarcane ditch is drenched with water before transplanting and is drenched, transplanting finishes and builds Small plastic shed above sugarcane ditch planting, according to line-spacing and Small plastic shed highly selects the film being dimensioned for be covered on Small plastic shed, and the top both sides that film is placed on seedling land, and both ends are opened Ventilating opening is stayed, screened negative is placed on outside film and by the ventilator hole cover of both ends, the transplanting same day opens at the top of Small plastic shed every 30~60 ㎝ One 2 ㎝ air hole of diameter, after transplanting 3~5 days, expands air hole to 10 ㎝ of diameter, transplanting and raises film completely after 8 days, Watering in two days is once;And apply based on the composite fertilizer of fertilizer 0.05% for the first time, once in a week;Cutting seedlings is once weekly;It is affixed by after 20 days 0.15% nitrogenous fertilizer or composite fertilizer;Sugarcane tissue-culture transplantation of seedlings transplants crop field in more than 40 days.
CN201711421940.8A 2017-12-25 2017-12-25 A kind of method for transplanting of sugarcane tissue-culture nursery Pending CN107980531A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711421940.8A CN107980531A (en) 2017-12-25 2017-12-25 A kind of method for transplanting of sugarcane tissue-culture nursery

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711421940.8A CN107980531A (en) 2017-12-25 2017-12-25 A kind of method for transplanting of sugarcane tissue-culture nursery

Publications (1)

Publication Number Publication Date
CN107980531A true CN107980531A (en) 2018-05-04

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108718981A (en) * 2018-08-14 2018-11-02 柳州市合联农业有限公司 A kind of method of sugar-cane tissue culture seedlings field production
CN108834809A (en) * 2018-07-19 2018-11-20 广西壮族自治区农业科学院甘蔗研究所 A kind of 2 years three sugarcane rotation cropping methods received
CN110476809A (en) * 2019-08-16 2019-11-22 广东省生物工程研究所(广州甘蔗糖业研究所) A kind of transportation resources of sugarcane tissue-culture rooted seedling
CN112314378A (en) * 2020-10-26 2021-02-05 广西壮族自治区农业科学院 Efficient sugarcane test-tube seedling transplanting method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101707979A (en) * 2009-11-30 2010-05-19 广西壮族自治区甘蔗研究所 Sugarcane tissue-culture-seedling bare-seedling field-transplanting method
CN102405837A (en) * 2011-09-06 2012-04-11 广西亚热带作物研究所试验站 Method for transplanting sugarcane tissue culture seedlings to fields from low tunnels
CN102498912A (en) * 2011-11-23 2012-06-20 云南省农业科学院甘蔗研究所 Method for transplanting sugar-cane tissue culture seedlings

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101707979A (en) * 2009-11-30 2010-05-19 广西壮族自治区甘蔗研究所 Sugarcane tissue-culture-seedling bare-seedling field-transplanting method
CN102405837A (en) * 2011-09-06 2012-04-11 广西亚热带作物研究所试验站 Method for transplanting sugarcane tissue culture seedlings to fields from low tunnels
CN102498912A (en) * 2011-11-23 2012-06-20 云南省农业科学院甘蔗研究所 Method for transplanting sugar-cane tissue culture seedlings

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834809A (en) * 2018-07-19 2018-11-20 广西壮族自治区农业科学院甘蔗研究所 A kind of 2 years three sugarcane rotation cropping methods received
CN108718981A (en) * 2018-08-14 2018-11-02 柳州市合联农业有限公司 A kind of method of sugar-cane tissue culture seedlings field production
CN110476809A (en) * 2019-08-16 2019-11-22 广东省生物工程研究所(广州甘蔗糖业研究所) A kind of transportation resources of sugarcane tissue-culture rooted seedling
CN112314378A (en) * 2020-10-26 2021-02-05 广西壮族自治区农业科学院 Efficient sugarcane test-tube seedling transplanting method

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Application publication date: 20180504