CN104335887A - Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid - Google Patents
Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid Download PDFInfo
- Publication number
- CN104335887A CN104335887A CN201410543538.7A CN201410543538A CN104335887A CN 104335887 A CN104335887 A CN 104335887A CN 201410543538 A CN201410543538 A CN 201410543538A CN 104335887 A CN104335887 A CN 104335887A
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- Prior art keywords
- seedling
- survival rate
- orchid
- seedlings
- transplanting survival
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention belongs to the technical field of flower seedling transplantation and in particular relates to a method for increasing the transplanting survival rate of tissue culture hybrid seedlings of orchid by a water culture process. According to the method, the tissue culture hybrid seedlings are transplanted and recovered by the characteristics that the root systems of the seedlings are in direct contact with nutritional liquid and can quickly absorb nutrition in the nutritional liquid in the water culture technology, so that the effects of reducing cost, saving labor and obtaining a relatively large amount of high-quality tissue culture hybrid seedlings of the orchid are achieved; as the seedlings are recovered by the water culture process, the number of new leaves, the number of new buds and the plant height increment of the seedlings are all superior to the growth condition of the tissue culture seedlings directly transferred into a matrix, the transplanting survival rate of tissue culture hybrid seedlings of the orchid is increased, the quality of nursery stock is improved, and the economic benefit of the orchid is increased.
Description
One, technical field
The invention belongs to flowers transplantation of seedlings technical field, be specifically related to a kind of method utilizing water planting mode to delay seedling raising orchid hybridization plantlet in vitro transplanting survival rate.
Two, background technology
Crossbreeding is the main path of orchid cultivation new varieties, has obtained between Some Species, intergeneric conjugal transfer by artificial hybridization, has cultivated the crossbreed of different pattern, flower pattern, tool fragrance, high resistance to cold and diseases.On a large scale, the commerciality application of tissue rapid propagation is also flourish throughout the country in the industrialized development of current China orchid test-tube plantlet, green house seedling.Transplanting as the last of group training is also a very important step, and the height of its survival rate has substantial connection with the height of the development of orchid industry and orchid quality.In raising orchid plantlet in vitro transplanting survival rate, have certain research, but mainly lay particular emphasis on the aspects such as the management of type of culture medium, transplanting medium, hardening, bottle seedling stalwartness and plantlet in vitro.But the method for raising plantlet in vitro transplanting survival rate conventional is at present time-consuming takes a lot of work, and transplanting survival rate is low, and seedling growth is weak.Grow in the environment of temperature control control light, afforest hardening if be allowed to condition in planting environment, grow and grow to seedlings of blooming, domestication, slow seedling, transplant most important.
Three, summary of the invention
The present invention need solve problem be:
How the method for raising orchid hybridization plantlet in vitro survival rate, namely take root the hybridization group training of cultivating under temperature control light control bar part for seedling by domestication, the cultivation of slow seedling immigration conventional substrate, and improve transplanting survival rate.
Technical scheme of the present invention is:
(1) selection and the process of plantlet in vitro is hybridized: growth selection is healthy and strong, the hybridization plantlet in vitro of having taken root, at glass greenhouse hardening 7d, with the medium of clean water root, the thiophanate methyl with 0.1% or carbendazim solution dry in the shade after soaking 30min to root system and turn white;
(2) seedling water planting: wrapped up by the root system mulch film of the hybridization group of cleaning and sterilizing training seedling, pass from foam cap hole, remove mulch film, what allow root system unfold puts into ready water planting container, allows nutrient solution submergence root system 2/3 in container; Nutrient solution prescription is: Ca (NO
3)
24H
2o 354mg/L, KNO
3117mg/L, MgSO
47H
2o 57.5mg/L, (NH
4)
2sO
4185mg/L, FeSO
47H
2o 27mg/L, EDTA-2Na37mg/L, H
3bO
34mg/L, MnSO
44H
2o 2mg/L, ZnSO
47H
2o 0.3mg/L, CuSO
45H
2o0.08mg/L, Na
2moO
42H
2o 0.03mg/L;
(3) plantlet in vitro is transplanted and management:
Seedling after sterilization is proceeded in ready water planting container, water planting container is inserted in the small plastic canopy of heat and moisture preserving, control temperature 20-25 DEG C, relative air humidity 80-90%, intensity of illumination is 0.8-1.5 ten thousand Lx, within every two weeks, spray a bactericide, bactericide is first holder or the carbendazim of 0.1%, within every about 10 days, change one time of nutrition liquid, to clean container when changing nutrient solution, process there being the seedling of rotten, note not damaging seedling, when temperature raises or evaporation discharge is very large, want to be immersed in wherein to Additional nutrient solution in culture vessel to keep root system in time, after 50d, transplanting survival rate reaches 96.7%, afterwards by seedling replanting to sphagna: cultivate in the mixed-matrix of import peat composed of rotten mosses mass ratio=2:1, after 60d, transplanting survival rate is 98.0%, and growth of seedling is good, the new number of blade, sprouting number, plant height increment is all better than the upgrowth situation directly moved into by plantlet in vitro in matrix.
The present invention compared with prior art beneficial effect is:
Instant invention overcomes the deficiency of traditional orchid hybridization plantlet in vitro transplanting method, take full advantage of water planting seedlings root and contact with nutrient solution, directly absorb the feature of the nutrient in nutrient solution, overcome the difficult point that orchid plantlet in vitro transplanting survival rate is low and seedling growth is weak.Pass through technique improvement, water planting is adopted to delay seedling new technology, not only transplanting survival rate reaches 98%, because water planting delays seedling, the new number of blade of seedling, sprouting number, plant height increment are all better than the upgrowth situation directly moved into by plantlet in vitro in matrix, improve transplanting survival rate and the nursery stock quality of orchid hybridization plantlet in vitro, and then improve the economic benefit of orchid.
Four, accompanying drawing explanation
Accompanying drawing is that water planting is delayed seedling and mixed-matrix and to be planted Chunlan and hybrid cymbidium cross hybrid seedling grows and root system development situation:
Fig. 1 water planting delays the seedling of seedling nutrient solution process.
Fig. 2 water planting delays the children of seedling clear water process.
The seedling of Fig. 3 mixed-matrix cultivating nutrient solution process.
The seedling of Fig. 4 mixed-matrix cultivation clear water process.
Five, embodiment
(1) hybridize the selection of plantlet in vitro and process: before transplanting, Chunlan and hybrid cymbidium are hybridized plantlet in vitro of taking root and train laboratory transfer to glass greenhouse booth hardening 7d from organizing.When transplanting growth selection healthy and strong, without the hybridization group training seedling of damage by disease and insect, remove rotten, old root etc., root clear water cleaned, then uses running water, then carry out disinfection with 0.1% thiophanate methyl or carbendazim solution immersion 30min.
(2) water planting container and nutrient solution prepare: water planting container is the wide-mouth jam jar of 200ml, fix bottle stopper with the cystosepiment of self-control boring as orchid.Water planting container is carried out cleaning and disinfecting and injects appropriate nutrient solution, nutrient solution prescription is: Ca (NO
3)
24H
2o 354mg/L, KNO
3117mg/L, MgSO
47H
2o 57.5mg/L, (NH
4)
2sO
4185mg/L, FeSO
47H
2o 27mg/L, EDTA-2Na 37mg/L, H
3bO
34mg/L, MnSO
44H
2o 2mg/L, ZnSO
47H
2o 0.3mg/L, CuSO
45H
2o 0.08mg/L, Na
2moO
42H
2o0.03mg/L.
(3) seedling water planting: the root system mulch film that the Chunlan of cleaning and sterilizing and hybrid cymbidium hybridization group train seedling is wrapped up, pass from foam cap hole, remove mulch film, what allow root system unfold puts into ready water planting container, allows nutrient solution submergence root system 2/3 in container.
(4) water planting seedling management: control temperature 20-25 DEG C, relative air humidity 80-90%, intensity of illumination is 0.8-1.5 ten thousand Lx.Within every two weeks, spray a bactericide (the first holder of 0.1% or carbendazim).Within every about 10 days, changing one time of nutrition liquid (original formulation), will clean container when changing nutrient solution, to there being the seedling of rotten to process, noting not damaging seedling.When temperature raises or evaporation discharge is very large, to be immersed in wherein to Additional nutrient solution in culture vessel to keep root system in time.After cultivating 50d, transplanting survival rate reaches 96.7%.Afterwards seedling is moved into sphagna: the import peat composed of rotten mosses=2:1 matrix is cultivated, and continuation mill water culture nutrient solution waters.After 60d, transplanting survival rate is 98.0%.
Claims (1)
1. improve a method for orchid hybridization plantlet in vitro transplanting survival rate, it is characterized in that adopting water planting to delay seedling to improve transplanting survival rate and seedling quality, be specifically made up of following steps:
(1) selection and the process of plantlet in vitro is hybridized: growth selection is healthy and strong, the hybridization plantlet in vitro of having taken root, at glass greenhouse hardening 7d, with the medium of clean water root, the thiophanate methyl with 0.1% or carbendazim solution dry in the shade after soaking 30min to root system and turn white;
(2) seedling water planting: wrapped up by the root system mulch film of the hybridization group of cleaning and sterilizing training seedling, pass from foam cap hole, remove mulch film, what allow root system unfold puts into ready water planting container, allows nutrient solution submergence root system 2/3 in container; Nutrient solution prescription is: Ca (NO
3)
24H
2o 354mg/L, KNO
3117mg/L, MgSO
47H
2o 57.5mg/L, (NH
4)
2sO
4185mg/L, FeSO
47H
2o 27mg/L, EDTA-2Na37mg/L, H
3bO
34mg/L, MnSO
44H
2o 2mg/L, ZnSO
47H
2o 0.3mg/L, CuSO
45H
2o0.08mg/L, Na
2moO
42H
2o 0.03mg/L;
(3) plantlet in vitro is transplanted and management:
Seedling after sterilization is proceeded in ready water planting container, water planting container is inserted in the small plastic canopy of heat and moisture preserving, control temperature 20-25 DEG C, relative air humidity 80-90%, intensity of illumination is 0.8-1.5 ten thousand Lx, within every two weeks, spray a bactericide, bactericide is first holder or the carbendazim of 0.1%, within every about 10 days, change one time of nutrition liquid, to clean container when changing nutrient solution, process there being the seedling of rotten, note not damaging seedling, when temperature raises or evaporation discharge is very large, want to be immersed in wherein to Additional nutrient solution in culture vessel to keep root system in time, after 50d, transplanting survival rate reaches 96.7%, afterwards by seedling replanting to sphagna: cultivate in the mixed-matrix of import peat composed of rotten mosses mass ratio=2:1, after 60d, transplanting survival rate is 98.0%.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191774A (en) * | 2015-11-04 | 2015-12-30 | 华南农业大学 | Water culturing method for nursery stock |
| CN107637494A (en) * | 2017-10-20 | 2018-01-30 | 三明市农业科学研究院 | A kind of method for improving oncidiumLuridum tissue-cultured seedling transplanting survival rate |
| CN108464207A (en) * | 2018-04-13 | 2018-08-31 | 中国水稻研究所 | A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate |
| CN110771486A (en) * | 2019-11-14 | 2020-02-11 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
| CN110959513A (en) * | 2018-09-28 | 2020-04-07 | 河北农业大学 | Novel water culture method for improving transplanting survival rate of tissue culture seedlings of pyrus betulaefolia |
| CN114521491A (en) * | 2022-02-25 | 2022-05-24 | 肇庆雅兰芳农业科技有限公司 | Method for breaking transplanting dormancy of cymbidium tissue culture seedlings and shortening production period |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191774A (en) * | 2015-11-04 | 2015-12-30 | 华南农业大学 | Water culturing method for nursery stock |
| CN107637494A (en) * | 2017-10-20 | 2018-01-30 | 三明市农业科学研究院 | A kind of method for improving oncidiumLuridum tissue-cultured seedling transplanting survival rate |
| CN108464207A (en) * | 2018-04-13 | 2018-08-31 | 中国水稻研究所 | A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate |
| CN110959513A (en) * | 2018-09-28 | 2020-04-07 | 河北农业大学 | Novel water culture method for improving transplanting survival rate of tissue culture seedlings of pyrus betulaefolia |
| CN110771486A (en) * | 2019-11-14 | 2020-02-11 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
| CN110771486B (en) * | 2019-11-14 | 2022-03-01 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
| CN114521491A (en) * | 2022-02-25 | 2022-05-24 | 肇庆雅兰芳农业科技有限公司 | Method for breaking transplanting dormancy of cymbidium tissue culture seedlings and shortening production period |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
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Application publication date: 20150211 |
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