CN103988762A - Water culturing and transplanting method for apple tissue culture seedlings - Google Patents
Water culturing and transplanting method for apple tissue culture seedlings Download PDFInfo
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- CN103988762A CN103988762A CN201410156599.8A CN201410156599A CN103988762A CN 103988762 A CN103988762 A CN 103988762A CN 201410156599 A CN201410156599 A CN 201410156599A CN 103988762 A CN103988762 A CN 103988762A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000012258 culturing Methods 0.000 title claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 29
- 238000004140 cleaning Methods 0.000 claims abstract description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 16
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- 235000011148 calcium chloride Nutrition 0.000 claims description 8
- 239000011790 ferrous sulphate Substances 0.000 claims description 8
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 8
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 8
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 239000011702 manganese sulphate Substances 0.000 claims description 8
- 235000007079 manganese sulphate Nutrition 0.000 claims description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 230000000050 nutritive effect Effects 0.000 claims description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- 239000002985 plastic film Substances 0.000 claims description 8
- 229920006255 plastic film Polymers 0.000 claims description 8
- 235000010333 potassium nitrate Nutrition 0.000 claims description 8
- 239000004323 potassium nitrate Substances 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 8
- 239000011686 zinc sulphate Substances 0.000 claims description 8
- 235000009529 zinc sulphate Nutrition 0.000 claims description 8
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
- 210000003608 fece Anatomy 0.000 claims description 4
- 244000037666 field crops Species 0.000 claims description 4
- 239000010871 livestock manure Substances 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 230000008719 thickening Effects 0.000 claims description 4
- 230000007704 transition Effects 0.000 claims description 4
- 238000002054 transplantation Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000004060 metabolic process Effects 0.000 abstract description 4
- 230000001651 autotrophic effect Effects 0.000 abstract description 2
- 230000029553 photosynthesis Effects 0.000 abstract description 2
- 238000010672 photosynthesis Methods 0.000 abstract description 2
- 230000009466 transformation Effects 0.000 abstract description 2
- 210000001339 epidermal cell Anatomy 0.000 abstract 1
- 235000011430 Malus pumila Nutrition 0.000 description 14
- 235000015103 Malus silvestris Nutrition 0.000 description 14
- 241000220225 Malus Species 0.000 description 12
- -1 0.9 part Chemical compound 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 239000002609 medium Substances 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 231100000252 nontoxic Toxicity 0.000 description 4
- 230000003000 nontoxic effect Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 3
- 238000004161 plant tissue culture Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 244000141359 Malus pumila Species 0.000 description 2
- 238000004173 biogeochemical cycle Methods 0.000 description 2
- 238000001784 detoxification Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229960002523 mercuric chloride Drugs 0.000 description 2
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 208000005156 Dehydration Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
Classifications
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- Y02P60/216—
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- Cultivation Of Plants (AREA)
Abstract
The invention discloses a water culturing and transplanting method for apple tissue culture seedlings. The method includes the steps of greenhouse exercising of rooting container seedlings, cleaning of the tissue culture seedlings, preparing of a water culturing facility, water culturing of the rooting seedlings and outdoor transplanting management. According to the water culturing and transplanting method for the apple tissue culture seedlings, due to the physiological feature that before the apple tissue culture seedlings are taken out of a container, complete cuticles are not formed on blades of the apple tissue culture seedlings and the effective photosynthesis cannot be conducted, nutrient solutions are used for water culturing, under an appropriate environment, the epidermal cells of the blades of the tissue culture seedlings are promoted to develop to be mature, transformation from heterotrophic metabolism to autotrophic metabolism is fast completed, and the purpose of improving the transplantation survival rate of the apple tissue culture seedlings is achieved. The technology is easy to implement and low in cost, and the transplantation survival rate can be 90 percent or more.
Description
Technical field
The present invention relates to field of plant tissue culture technique, specifically a kind of apple group that relates to is trained the method that the seedling water planting of taking root is transplanted, and this transplanting method can make transplanting survival rate reach more than 90%, and management is simple, and production cost is low.
Background technology
In recent years, along with scientific and technical fast development, plant tissue culture technique has entered the production application stage, is widely used in flowers, the large-scale rapid propagation in vitro of fruit tree, and test-tube plantlet has formed industrialization in international market.Tissue is cultivated the important means as apple detoxification, and realizes the fast breeding of nursery stock, replaces the cultivation of common seedling to apply more and more extensive with group training detoxic seedling, and application prospect is also very wide.
Plant tissue culture detoxifying fast breeding was produced in manual control condition's next year four seasons, and obtained at short notice a large amount of group training detoxification nursery stocks.But the transplanting of group training seedling, from indoor to outdoor, is transformed to natural environmental condition gradually by the low light level in bottle, constant temperature, nearly saturated humidity, gnotobasis, and technical difficulty is larger.Due to the blade of differentiation under high humidity, the low light level, heterotrophism condition and growth, leaf cuticle, wax coat are undeveloped, leaf texture gap is large, palisade tissue is thin, and the conducting system of stem is grown imperfection in addition, and root is without absorption function or extremely low, when transplanting high ambient temperature or humidity when lower and easily causing a large amount of dehydrations of blade, and that root system can not absorb water is effectively supplementary, therefore it is withered very easily to wilt, easily cause the failure of transplanting.Especially in common greenhouse by solar heat or plastic tunnel, transplant, be subject to the impact of Various Seasonal temp. and humidity, transplanting effect has very large difference.
Summary of the invention
Technical problem to be solved by this invention is the water planting transplanting method that a kind of apple group training seedling that improves apple group training transplantation of seedlings survival rate is provided for transplanting the poor problem of effect in prior art.
Adopt following technical scheme for solving technical problem of the present invention:
A water planting transplanting method for apple group training seedling, comprises that seedling, the cleaning of group training seedling, the preparation of solution culture facilities, the seedling water planting of taking root, outdoor point of cultivation management process are practiced in the greenhouse of bottle seedling of taking root:
The indoor experienced seedling of a, bottle seedling of taking root: by taking out from culturing room through culture of rootage, the bottle seedling that reaches transplanting standard, be placed in greenhouse by solar heat, close bottle and take exercise after 5～7 days, throw off blake bottle sealing, in bottle, inject the high running water of 0.3-0.5cm, then take exercise 3～5 days, then carry out water planting;
The cleaning of b, group training seedling: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium that is attached to root with clear water off, remove lower blade, as far as possible the damage of minimizing group training shoot root, stem, leaf, after cleaning, dry in the shade the moisture content on group training seedling leaf surface;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 80～100cm, long 600～800cm puts successively in greenhouse, passes into nutrient solution in hydroponic box, places the cystosepiment of accomplishing fluently aperture of thick 0.3～1cm for fixing young plant on nutrient solution; Nutrient solution is made up of water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate;
The water planting of d, group training seedling: plant in accomplishing fluently in advance the cystosepiment of aperture cleaning the group training seedling of taking root drying well, add the nutrient solution that 10-15 cm is high in incubator, cystosepiment is floated on nutrient solution;
E, plastic film cover: incubator mouth is all covered with plastic film, the space transition that makes Rooted Cuttings be placed in sealing is cultivated 3～5 days, all threw off film with 3～5 day time again, the growth of ventilating, in the time of water planting 30～40 days, main root lengthens growth, hair root increases, it is green that blade turns, and when thickening, extracts a point cultivation;
F, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, spray water in time or water, make nursery stock adapt to gradually open country growing environment, while growing to 10-15cm Deng seedling in nutritive cube, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
The mass ratio of the each raw material consumption of described nutrient solution is: 1000 parts, water, potassium nitrate 0.8-1.0 part, ammonium nitrate 0.7-0.9 part, potassium dihydrogen phosphate 0.75-0.95 part, magnesium sulfate 0.16-0.20 part, calcium chloride 0.20-0.24 part, manganese sulphate 0.08-0.14 part, zinc sulphate 0.02-0.06 part, ferrous sulfate 0.16-0.20 part.
In described step c, between each incubator, be connected with supple-management, by water pump, nutrient solution entirety circulated.
The present invention does not form perfect cuticula according to apple group training seedling its blade before bottle outlet, can not carry out the physiological characteristics such as effective photosynthesis, use suspension culture, make group training seedling under compared with adapt circumstance condition, promote blade table chrotoplast to reach maturity, complete quickly the transformation from heterotroph metabolism to autotrophic type metabolism, reach and improve the object that apple group training transplantation of seedlings survives.This technology is implemented simple, and cost is low, and transplanting survival rate reaches more than 90%.
Embodiment
embodiment 1
Apple tissue culture technology comprise explant foundation, expand the links such as numerous cultivation, culture of rootage, transplanting, wherein:
The foundation of explant: the annual treelet terminal bud of the vigorous good apple tree of clip grown in field or lateral bud, use running water flow wash, 74% alcohol surface sterilization, with 0.1% mercuric chloride sterilizing, after finally clean with aseptic water washing, on desinfection chamber superclean bench, receive on inducing culture and in culturing room, induce differentiation, obtain nontoxic stem apex as cultivation explant;
Expand numerous cultivation: the nontoxic stem apex that said method is obtained is cut into the stem section of 2cm, be inoculated into and expand in breeding culture medium on desinfection chamber superclean bench, every bottle of medium connects 4 buds, is placed on culturing room and expands numerous cultivation;
Take root: numerous after some when nursery stock expansion, select healthy and strong strong sprout of growing, on desinfection chamber superclean bench, be cut into 2cm stem apex and be inoculated on root media, every bottle of 5 strains, are placed on culturing room and carry out culture of rootage, when after more than 90% little seedling rooting, can pull out culturing room and transplant.
Apple group training seedling Greenhouse Water Culture transplantation technique comprises that seedling is practiced in the greenhouse of bottle seedling of taking root, group is trained the cleaning of seedling, the preparation of solution culture facilities, the configuration of nutrient solution, the seedling water planting of taking root, outdoor point of cultivation management process:
The indoor experienced seedling of a, bottle seedling of taking root: by taking out from culturing room through culture of rootage, the bottle seedling that reaches transplanting standard, be placed in greenhouse by solar heat, close bottle and take exercise after 7 days, throw off blake bottle sealing, in bottle, inject the high running water of 0.3cm, then take exercise 3 days, then carry out water planting;
The cleaning of b, group training seedling: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium that is attached to root with clear water off, remove lower blade, as far as possible the damage of minimizing group training shoot root, stem, leaf, after cleaning, dry in the shade the moisture content on group training seedling leaf surface;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 80cm, long 800cm puts successively in greenhouse, between each incubator, is connected with supple-management, by water pump, nutrient solution entirety is circulated, and places the cystosepiment of thick 1cm for fixing young plant on nutrient solution;
The configuration of d, nutrient solution: nutrient solution is by being to form with water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate, and the mass ratio of each raw material consumption is: 1000 parts, water, 0.8 part, potassium nitrate, 0.9 part, ammonium nitrate, 0.75 part of potassium dihydrogen phosphate, 0.16 part, magnesium sulfate, 0.24 part, calcium chloride, 0.14 part of manganese sulphate, 0.02 part, zinc sulphate, 0.16 part, ferrous sulfate;
The water planting of e, group training seedling: plant in accomplishing fluently in advance the cystosepiment of aperture cleaning the group training seedling of taking root drying well, add the nutrient solution that 10cm is high in incubator, cystosepiment is floated on nutrient solution, open water pump, Nutrient Cycle is flowed;
F, plastic film cover: incubator mouth is all covered with plastic film, the space transition that makes Rooted Cuttings be placed in sealing is cultivated 3 days, all threw off film with 3 day time again, the growth of ventilating, in the time of water planting 40 days, main root lengthens growth, hair root increases, it is green that blade turns, and when thickening, extracts a point cultivation;
G, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, spray water in time or water, make nursery stock adapt to gradually open country growing environment, while growing to 10cm Deng seedling in nutritive cube, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
embodiment 2
Apple tissue culture technology comprise explant foundation, expand the links such as numerous cultivation, culture of rootage, transplanting, wherein:
The foundation of explant: the annual treelet terminal bud of the vigorous good apple tree of clip grown in field or lateral bud, use running water flow wash, 74% alcohol surface sterilization, with 0.1% mercuric chloride sterilizing, after finally clean with aseptic water washing, on desinfection chamber superclean bench, receive on inducing culture and in culturing room, induce differentiation, obtain nontoxic stem apex as cultivation explant;
Expand numerous cultivation: the nontoxic stem apex that said method is obtained is cut into the stem section of 2cm, be inoculated into and expand in breeding culture medium on desinfection chamber superclean bench, every bottle of medium connects 3 buds, is placed on culturing room and expands numerous cultivation;
Take root: numerous after some when nursery stock expansion, select healthy and strong strong sprout of growing, on desinfection chamber superclean bench, be cut into 2cm stem apex and be inoculated on root media, every bottle of 4 strains, are placed on culturing room and carry out culture of rootage, when after more than 90% little seedling rooting, can pull out culturing room and transplant.
Apple group training seedling Greenhouse Water Culture transplantation technique comprises that seedling is practiced in the greenhouse of bottle seedling of taking root, group is trained the cleaning of seedling, the preparation of solution culture facilities, the configuration of nutrient solution, the seedling water planting of taking root, outdoor point of cultivation management process:
The indoor experienced seedling of a, bottle seedling of taking root: by taking out from culturing room through culture of rootage, the bottle seedling that reaches transplanting standard, be placed in greenhouse by solar heat, close bottle and take exercise after 5 days, throw off blake bottle sealing, in bottle, inject the high running water of 0.5cm, then take exercise 5 days, then carry out water planting;
The cleaning of b, group training seedling: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium that is attached to root with clear water off, remove lower blade, as far as possible the damage of minimizing group training shoot root, stem, leaf, after cleaning, dry in the shade the moisture content on group training seedling leaf surface;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 100cm, long 600cm, in greenhouse, put successively, between each incubator, be connected with supple-management, by water pump, nutrient solution entirety circulated, on nutrient solution, place the cystosepiment of thick 0.3cm for fixing young plant;
The configuration of d, nutrient solution: nutrient solution is by being to form with water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate, and the mass ratio of each raw material consumption is: 1000 parts, water, 1.0 parts, potassium nitrate, 0.7 part, ammonium nitrate, 0.95 part of potassium dihydrogen phosphate, 0.20 part, magnesium sulfate, 0.20 part, calcium chloride, 0.08 part of manganese sulphate, 0.06 part, zinc sulphate, 0.20 part, ferrous sulfate;
The water planting of e, group training seedling: plant in accomplishing fluently in advance the cystosepiment of aperture cleaning the group training seedling of taking root drying well, add the nutrient solution that 15cm is high in incubator, cystosepiment is floated on nutrient solution, open water pump, Nutrient Cycle is flowed;
F, plastic film cover: incubator mouth is all covered with plastic film, the space transition that makes Rooted Cuttings be placed in sealing is cultivated 5 days, all threw off film with 5 day time again, the growth of ventilating, in the time of water planting 30 days, main root lengthens growth, hair root increases, it is green that blade turns, and when thickening, extracts a point cultivation;
G, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, spray water in time or water, make nursery stock adapt to gradually open country growing environment, while growing to 15cm Deng seedling in nutritive cube, band soil mud enters land for growing field crops, with reference to field seedling raising management by methods.
Claims (3)
1. a water planting transplanting method for apple group training seedling, is characterized in that comprising take root the greenhouse white silk seedling of bottle seedling, the cleaning of group training seedling, the preparation of solution culture facilities, the seedling water planting of taking root, outdoor point of cultivation management process:
The indoor experienced seedling of a, bottle seedling of taking root: by taking out from culturing room through culture of rootage, the bottle seedling that reaches transplanting standard, be placed in greenhouse by solar heat, close bottle and take exercise after 5～7 days, throw off blake bottle sealing, in bottle, inject the high running water of 0.3-0.5cm, then take exercise 3～5 days, then carry out water planting;
The cleaning of b, group training seedling: the bottle seedling tweezers of having taken exercise are taken out gently from bottle, wash the medium that is attached to root with clear water off, remove lower blade, as far as possible the damage of minimizing group training shoot root, stem, leaf, after cleaning, dry in the shade the moisture content on group training seedling leaf surface;
The preparation of c, solution culture facilities: the dark 20cm of hydroponic box, wide 80～100cm, long 600～800cm puts successively in greenhouse, passes into nutrient solution in hydroponic box, places the cystosepiment of accomplishing fluently aperture of thick 0.3～1cm for fixing young plant on nutrient solution; Nutrient solution is made up of water, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, manganese sulphate, zinc sulphate, ferrous sulfate;
The water planting of d, group training seedling: plant in accomplishing fluently in advance the cystosepiment of aperture cleaning the group training seedling of taking root drying well, add the nutrient solution that 10cm-15 cm is high in incubator, cystosepiment is floated on nutrient solution;
E, plastic film cover: incubator mouth is all covered with plastic film, the space transition that makes Rooted Cuttings be placed in sealing is cultivated 3～5 days, all threw off film with 3～5 day time again, the growth of ventilating, in the time of water planting 30～40 days, main root lengthens growth, hair root increases, it is green that blade turns, and when thickening, extracts a point cultivation;
F, outdoor point of cultivation management: by the seedling of water planting in greenhouse, it is soil that individual plant moves into matrix: in the nutritive cube of decomposed manure 1:0.3, move in outdoor shade, spray water in time or water, make nursery stock adapt to gradually open country growing environment, while growing to 10-15cm Deng seedling in nutritive cube, be with soil mud to enter land for growing field crops, with reference to field seedling raising management by methods.
2. the water planting transplanting method of a kind of apple group training seedling according to claim 1, is characterized in that: the mass ratio of the each raw material consumption of described nutrient solution is: 1000 parts, water, potassium nitrate 0.8-1.0 part, ammonium nitrate 0.7-0.9 part, potassium dihydrogen phosphate 0.75-0.95 part, magnesium sulfate 0.16-0.20 part, calcium chloride 0.20-0.24 part, manganese sulphate 0.08-0.14 part, zinc sulphate 0.02-0.06 part, ferrous sulfate 0.16-0.20 part.
3. the water planting transplanting method of a kind of apple group training seedling according to claim 1 and 2, is characterized in that: in described step c, between each incubator, be connected with supple-management, by water pump, nutrient solution entirety circulated.
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Cited By (8)
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| CN104335887A (en) * | 2014-10-14 | 2015-02-11 | 金陵科技学院 | Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid |
| CN104604661A (en) * | 2015-03-02 | 2015-05-13 | 吴国强 | Method for potting daphone odora varmargmata |
| CN105123479A (en) * | 2015-09-07 | 2015-12-09 | 天水市果树研究所 | Water culture transplanting method for cherry tissue culture seedlings |
| CN105900662A (en) * | 2016-05-12 | 2016-08-31 | 山东理工大学 | Soilless half-hydroponic planting method of organic Chinese chives |
| CN106212237A (en) * | 2016-08-08 | 2016-12-14 | 天水市果树研究所 | A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling |
| CN110679457A (en) * | 2019-11-14 | 2020-01-14 | 中国林业科学研究院亚热带林业研究所 | A method for improving the transplanting survival rate of N. benthamiana tissue culture seedlings |
| CN112167034A (en) * | 2020-08-07 | 2021-01-05 | 兰婷 | Method for quickly breeding blueberries and natural light microcirculation cultivation box |
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| CN104335887A (en) * | 2014-10-14 | 2015-02-11 | 金陵科技学院 | Method for increasing transplanting survival rate of tissue culture hybrid seedlings of orchid |
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| CN106212237A (en) * | 2016-08-08 | 2016-12-14 | 天水市果树研究所 | A kind of outside sprout-cultivating-bottle method of Tissue-cultured apple seedling |
| CN106212237B (en) * | 2016-08-08 | 2021-05-04 | 天水市果树研究所 | Ex-bottle rooting method for apple tissue culture seedlings |
| CN110679457A (en) * | 2019-11-14 | 2020-01-14 | 中国林业科学研究院亚热带林业研究所 | A method for improving the transplanting survival rate of N. benthamiana tissue culture seedlings |
| CN112167034A (en) * | 2020-08-07 | 2021-01-05 | 兰婷 | Method for quickly breeding blueberries and natural light microcirculation cultivation box |
| CN113647318A (en) * | 2021-09-06 | 2021-11-16 | 北京农学院 | A method for promoting the occurrence of new roots of Chinese chestnut hydroponics |
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