CN108464207A - A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate - Google Patents
A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate Download PDFInfo
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- CN108464207A CN108464207A CN201810331778.9A CN201810331778A CN108464207A CN 108464207 A CN108464207 A CN 108464207A CN 201810331778 A CN201810331778 A CN 201810331778A CN 108464207 A CN108464207 A CN 108464207A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses it is a kind of improve rice tissue-cultured seedling transplanting survival rate cultural method, by tissue-cultured seedling cleaning, HORMONE TREATMENT, soil prepare, transplanting and environmental management and water and fertilizer management and etc. completion.The combination that the present invention passes through the innovation and auxin application technology of agronomic measures, solve the problems, such as that the survival rate after rice tissue culture transplantation of seedlings is substantially not guaranteed, using the survival rate after rice tissue culture transplantation of seedlings after this technology invention 85% or more, rice tissue-cultured seedling well developed root system after transplanting, robust plant, the percentage of earbearing tiller are significantly improved with setting percentage.
Description
Technical field
The invention belongs to micropropagation of plants technical fields, and in particular to a kind of to improve rice tissue-cultured seedling transplanting survival rate
Cultural method.
Background technology
Tissue cultures are under the aseptic condition artificially created, by isolated organ (such as root, stem, leaf, stem section, the original of plant
Raw plastid), tissue or cell be placed in culture medium, and be placed in suitable environment, carry out continuous culture to obtain cell, tissue
Or the technology of individual.This technology is widely used to the research of agricultural and biology, medicine.Rice tissue-cultured seedling is exactly to utilize tissue
The rice transformation seedling that culture technique obtains generally is grown in sterilizing and closed test tube or culture bottle, stringent
It is grown under the conditions of light temperature etc..
Rice tissue-cultured seedling is very young tender, is not easy to survive after transplanting (removing closed test tube or culture bottle).Using tradition side
Method, the survival rate after rice tissue culture transplantation of seedlings is substantially not guaranteed, and the survival rate after transplanting under normal circumstances is less than 50%.Even if
It survives, late growth is slow, and the percentage of earbearing tiller is low, and tassel is small.
Invention content
The present invention is in view of the defects existing in the prior art, it is therefore intended that is not easy the skill survived after solving rice tissue culture transplantation of seedlings
Art problem, provides a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate, and rice can be greatly improved in the technology of the present invention
Survival rate after tissue culture transplantation of seedlings, the rice tissue-cultured seedling well developed root system after transplanting, robust plant.
The present invention is realized especially by following technical scheme：
A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate, includes the following steps：
1) tissue-cultured seedling cleans
It after impregnating tissue-cultured seedling 2 days using International Rice institute nutrient solution prescription, is cleaned with 20-25 DEG C of Natural Water, removes tissue culture
The nutrient solution of seedling root prevents the generation that tissue-cultured seedling root system goes mouldy after transplanting；
2) HORMONE TREATMENT
The tissue-cultured seedling cleaned is put into test tube, the Natural Water containing abscisic acid is added not have root, in greenhouse shade item
It is cultivated 1-2 days under part, in seedling foliage-spray Gibberellins solution before transplanting, keeps temperature and with crape net sunshade；
3) soil prepares
With water paddy soil, per 7~10 kilograms of barreled dry ground, after dress soil plus water and smash to pieces uniformly, after in greenhouse stand 2
It, so as to soil temperature raising and surface dust sedimentation equilibrium；
4) transplanting and environmental management
It is inserted for single this kind when transplanting, transplanting depth just covers rice shoot root with soil to be advisable, and 1-2 centimetres is filled after shoot transplanting equipment
Shallow water after a week with the sunshade of crape net removes crape net and is cultivated under greenhouse natural lighting；
5) water and fertilizer management
Shallow water is filled after transplanting after 4 weeks and applies 10-15/basin of compound fertilizer dedicated to paddy, falls naturally after doing and keeps alternation of wetting and drying
Soil environment, fertilising after a week can field planting.
The Natural Water is by the sun according to the tap water for shining 5 days.
The content containing abscisic acid is 10~100mg/L in the Natural Water.
A concentration of 1~10mg/L of the Gibberellins solution, Study On Spraying Condition be per seedling foliage-spray Gibberellins solution 2~
5ml。
The condition of the crape net sunshade is to reach 1/10 intensity of illumination of normal illumination, to avoid direct sunlight.
Further include after transplanting 15 days in the transplanting and environmental management plus shallow water weed it is native primary, weed after soil fall naturally do to
Soil goes up water again after there are fine cracks, keeps alternation of wetting and drying soil environment later.
Beneficial effects of the present invention are：
The technology of the present invention is efficiently solved by the combination of the innovation and auxin application technology of agronomic measures
Problem is stated, using rice tissue-cultured seedling of the survival rate after rice tissue culture transplantation of seedlings after this technology invention after 85% or more, transplanting
Well developed root system, robust plant, the percentage of earbearing tiller are significantly improved with setting percentage.
Specific implementation mode
Below in conjunction with specific embodiment of the present invention, real technical solution of the invention is clearly and completely described, is shown
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
The present embodiment be directed to rice tissue culture transplantation of seedlings after survival rate substantially there is no guarantee that, under normal circumstances transplant after at
Motility rate is less than 50%, even if surviving, late growth is slow, and the percentage of earbearing tiller is low, and the small technological deficiency of tassel passes through agronomic measures
Innovation and auxin application technology combination, provide it is a kind of improve rice tissue-cultured seedling transplanting survival rate culture side
Method, the effective solution above problem, is completed especially by following steps：
1) tissue-cultured seedling cleans
Nutrition vacuole tissue-cultured seedling 2 days (International Rice institute nutrient solution prescription) is first used, then (is passed through with 20-25 degree " Natural Water "
The sun is according to the tap water for shining 5 days) cleaning, the culture medium (agarose etc.) of removal tissue-cultured seedling root, tissue-cultured seedling root after prevention transplanting
It is the generation gone mouldy, rear Ensure Liquid liquid did not had root.
2) HORMONE TREATMENT：
Tissue-cultured seedling (the step 1) cleaned is put into test tube, and " Natural Water " is added not have root, and 10 are contained in added " Natural Water "
The abscisic acid (ABA) of~100mg/L, adapts to 1-2 days before transplanting under greenhouse shading condition.First 5 hours of transplanting, per seedling blade face
2~5ml of Gibberellins solution of 1~10mg/L is sprayed, environment temperature maintains 20-25 degree, (reaches normal light with crape net sunshade
According to 1/10 intensity of illumination, to avoid direct sunlight).
3) soil prepares
The depth of potting bucket is 30-50 centimetres, 30-40 centimetres of diameter.It is public per barreled dry ground 7~10 with water paddy soil
Jin (dry ground is from 10~15cm of top of bucket), dress soil after plus water and smash to pieces uniformly, after greenhouse stand 2 days, so as to soil temperature raising
With surface dust sedimentation equilibrium, greenhouse temperature is maintained at 20-25 degree.
4) transplanting and environmental management
It is inserted for single this kind when test tube transplantation of seedlings, transplanting depth just covers rice shoot root with soil to be advisable, and is filled after shoot transplanting equipment
1-2 centimetres of shallow water, with crape net sunshade (reaching 1/10 intensity of illumination of normal illumination, to avoid direct sunlight) one week, after
Crape net (20-25 degree) under greenhouse natural lighting is removed to cultivate.Transplanting adds shallow water to weed native (loosening the soil) once with ventilation after 15 days
Oxygenation, while removing the withered rotten branches and leaves in rice strain, weed after soil fall naturally do there are fine cracks to soil after upper water again, Zhi Houbao
Hold alternation of wetting and drying soil environment.If air natural temperature at this time within the scope of 20-30, can move to potting outdoor natural
It is grown in environment.
5) water and fertilizer management
Tissue-cultured seedling has been completed to shake down and start normal growth tiller after 4 weeks after transplanting, fills shallow water at this time and applies
10-15/basin of compound fertilizer dedicated to paddy falls after doing and keeps the soil environment of alternation of wetting and drying naturally.Fertilising after a week can be seedling
Transplantation of seedlings can also keep potted plant growth (to be applicable in rice shoot negligible amounts to field planting (being applicable in a fairly large number of situation of rice shoot)
The case where), liquid manure and disease pest and weed management are with general Production of Large Fields, until harvest.
Embodiment 2
The transplanting for implementing 7 batches of tissue-cultured seedling altogether in China Paddy Rice Inst Fuyang base in 2017, transplanted seedling is in temperature control temperature
Room carries out, and for temperature control in greenhouse range at 20~30 degree, natural lighting is simultaneously equipped with shading net.Using the abscisic acid of 30mg/L
(ABA) the first 5 hours Gibberellins solution 2ml per seedling foliage-spray 3mg/L are transplanted in solution leaching root 2 days.Experimental result such as 1 He of table
2。
20 days survival rates after 1 tissue culture transplantation of seedlings of table
| Number batch | Tissue-cultured seedling quantity (strain) | Survival seedling quantity (strain) after transplanting | Survival rate % after transplanting 20 days |
| CN-170215-Pr-67 | 55 | 48 | 87.3 |
| CN-170325-Pr-59 | 80 | 68 | 85.0 |
| CN-170412-Pr-59 | 60 | 55 | 91.7 |
| CN-170412-Ar-67 | 91 | 80 | 87.9 |
| CN-170509-Si-59 | 95 | 83 | 87.4 |
| CN-170509-Ir-59 | 45 | 39 | 86.7 |
| CN-170606-Sn-59 | 174 | 161 | 92.5 |
| It amounts to | 600 | 534 | Average survival 89.0% |
As can be seen from Table 1, the survival rate of 20 days tissue-cultured seedling is 85%~92.5% after being transplanted using this technology, it is average at
Motility rate is 89.0%.
The percentage of earbearing tiller and setting percentage (every clump) of the genetic transformation strain of 2 rice Nipponbare of table
As can be seen from Table 2, the tissue-cultured seedling percentage of earbearing tiller 41.5%~48.4 after routine techniques transplanting, the average percentage of earbearing tiller are
44.5%；And it is 60.0%~68.1% to apply the tissue-cultured seedling percentage of earbearing tiller after this technology transplanting, the average percentage of earbearing tiller is 62.7%.Knot
Real rate similarly, is promoted by 25.8%~39.7% to 60.7%~80.4%, average setting percentage is also increased to by 29.9%
67.2%.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate, which is characterized in that include the following steps：
1) tissue-cultured seedling cleans
It after impregnating tissue-cultured seedling 2 days using International Rice institute nutrient solution prescription, is cleaned with 20-25 DEG C of Natural Water, removes tissue-cultured seedling root
The nutrient solution in portion prevents the generation that tissue-cultured seedling root system goes mouldy after transplanting；
2) HORMONE TREATMENT
The tissue-cultured seedling cleaned is put into test tube, the Natural Water containing abscisic acid is added not have root, under greenhouse shading condition
Culture 1-2 days, in seedling foliage-spray Gibberellins solution before transplanting, holding temperature and with crape net sunshade；
3) soil prepares
With water paddy soil, per 7~10 kilograms of barreled dry ground, after dress soil plus water and smash to pieces uniformly, after in greenhouse stand 2 days, with
Just soil temperature raising and surface dust sedimentation equilibrium；
4) transplanting and environmental management
It is inserted for single this kind when transplanting, transplanting depth just covers rice shoot root with soil to be advisable, and 1-2 centimetres of filling is shallow after shoot transplanting equipment
Water after a week with the sunshade of crape net removes crape net and is cultivated under greenhouse natural lighting；
5) water and fertilizer management
Shallow water is filled after transplanting after 4 weeks and applies 10-15/basin of compound fertilizer dedicated to paddy, falls naturally after doing and keeps the soil of alternation of wetting and drying
Earth environment, fertilising after a week can field planting.
2. a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate according to claim 1, which is characterized in that institute
The Natural Water stated is by the sun according to the tap water for shining 5 days.
3. a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate according to claim 1, which is characterized in that institute
The content of abscisic acid is 10~100mg/L in the Natural Water containing abscisic acid stated.
4. a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate according to claim 1, which is characterized in that institute
A concentration of 1~the 10mg/L for the Gibberellins solution stated, Study On Spraying Condition are per 2~5ml of seedling foliage-spray Gibberellins solution.
5. a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate according to claim 1, which is characterized in that institute
The condition for the crape net sunshade stated is to reach 1/10 intensity of illumination of normal illumination, to avoid direct sunlight.
6. a kind of cultural method improving rice tissue-cultured seedling transplanting survival rate according to claim 1, which is characterized in that institute
Further include that shallow water is added to weed soil once after transplanting 15 days in the transplanting and environmental management stated, weeds after soil to fall naturally and do to soil appearance carefully
Water is gone up after small crack again, keeps alternation of wetting and drying soil environment later.
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Cited By (1)
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