CN102090329B - Method for transplanting Damascus rose tissue culture seedling - Google Patents
Method for transplanting Damascus rose tissue culture seedling Download PDFInfo
- Publication number
- CN102090329B CN102090329B CN2010105375585A CN201010537558A CN102090329B CN 102090329 B CN102090329 B CN 102090329B CN 2010105375585 A CN2010105375585 A CN 2010105375585A CN 201010537558 A CN201010537558 A CN 201010537558A CN 102090329 B CN102090329 B CN 102090329B
- Authority
- CN
- China
- Prior art keywords
- transplanting
- seedling
- tissue culture
- illumination
- humidity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for transplanting a Damascus rose tissue culture seedling, comprising the following steps of: hardening off the Damascus rose tissue culture seedling for a week; then taking out the tissue culture seedling from a culture bottle; cleaning a root culture medium with clear water; directly transplanting the root culture medium into a hole disc filled with a transplanting substrate; setting and then irrigating a 0.1% carbendazol water solution once; irrigating again every other 5 days; continuously irrigating three times; covering a film on a seed bed, and uncovering the film every day for ventilating, wherein the culture temperature is 20-23 DEG C, the humidity is 90-95%, the shading light rate is 60-70%, and the illumination is 6,000-8,000Lx; irrigating an MS (Murashige and Skoog) culture medium which is diluted ten times every other three days once to promote the root system growth and the leaf germination; and after new leaves are grown, reducing the humidity to 70-80%, enhancing the illumination to 8,000-12,000 Lx and controlling the culture temperature to be 23-28 DEG C.
Description
Technical field
The present invention relates to plant tissue cultivation method field; Damask Rose cultivation method field particularly.
Background technology
Damask Rose (Rosa damascena) is shrub.Originate in Asia Minor, in southern Europe cultivation long, for perfume material processed, economic worth is very high.China various places are existing introducing and planting in recent years, for viewing and admiring and extract essence.Its propagation method mainly is cottage propagation, and survival rate is low, and rootage duration is long, and offspring's quality descends.Also have at present and use method for plant tissue culture to breed, but group training transplantation of seedlings survival rate is low, has greatly limited the batch production production of Damask Rose.
Summary of the invention
The present invention is for solving the method that the breeding cycle is long, technical problem that survival rate is low provides a kind of suitable transplanting Damascus rose tissue culture seedling, in order to improve the survival rate of Damask Rose.
The present invention is that the technical scheme of taking is: method for transplanting Damascus rose tissue culture seedling, the Damascus rose tissue culture seedling seedling is in the greenhouse hardening after one week, taking-up group training seedling from blake bottle, with clear water with root medium wash clean, directly transplant on the cave dish that transplanting medium is housed, water the carbendazim aqueous solution of one time 0.1% (volume volume ratio) after the field planting, watered once in 5 days again at every interval, water continuously 3 times, film is added a cover in the seedbed, 20～23 ℃ of cultivation temperature, humidity 90%～95%, shading 60～70%, illumination 6000～8000lx raises membrane gas treatment every day; Once dilute the MS liquid nutrient medium of 10 times (volume volume ratios) every pouring in 3 days, promote the sprouting of root growth and leaf; After young leaves was extracted out, humidity was reduced to 70～80%, and illumination is enhanced to 8000～12000Lx, and cultivation temperature is 23～28 ℃, and illumination is 8000～12000Lx.
Described cultivation matrix is the mixed-matrix of peat, perlite, river sand, and its volume ratio is 3～4: 2～3: 1.
Advantage and good effect that the present invention has are: adopt transplanting method of the present invention, improved the survival rate of large Ma Shi rose, and the growth conditions of plant is good.
Embodiment
This experiment is divided into experimental group and control group.Each processing of experimental group and control group are all chosen 500 strain groups training seedling as experiment material.
The experimental group experimental technique: the Damascus rose tissue culture seedling seedling is in the greenhouse hardening after one week, taking-up group training seedling from blake bottle, use clear water with root medium wash clean, directly transplant on the cave dish that transplanting medium is housed, transplanting medium is peat, river sand, perlitic mixed-matrix, and its volume ratio sees Table 1.Water the carbendazim aqueous solution of one time 0.1% (volume volume ratio) after the field planting of group training seedling, watered once in 5 days again at every interval, waters continuously 3 times, and film is added a cover in the seedbed.20～23 ℃ of cultivation temperature, humidity 90%～95%, shading 60～70%, illumination 6000～8000lx raises membrane gas treatment every day; Once dilute 10 times MS liquid nutrient medium (not adding sucrose) every pouring in 3 days, promote the sprouting of root growth and leaf.After young leaves was extracted out, humidity was reduced to 70～80%, and illumination is enhanced to 8000～12000Lx, and cultivation temperature is 23～28 ℃.
Table 1 cultivation matrix kind and proportioning
The control group experimental technique: the Damascus rose tissue culture seedling seedling is in the greenhouse hardening after one week, and taking-up group training seedling uses clear water with root medium wash clean from blake bottle, then uses 0.1% carbendazim solution soaking disinfection 20 minutes.Group after sterilization training transplantation of seedlings on the cave dish that river sand is housed, is not watered 0.1% carbendazim solution after the field planting.Condition of culture and manage same experimental group.
The cultivation method that adopts experimental group after about 20 days seedling can extract young leaves out.Adopt the sterilization method of pouring carbendazim more to be conducive to group training seedling rooting in the experimental group.Statistical experiment result after two months, statistical indicator comprise and survive strain number and average plant height.The result is as follows:
Table 2 experimental group and control group cultivation method are transplanted the impact of effect on Damask Rose
Interpretation of result:
As can be seen from Table 2, the group of experimental group training shoot survival percent, average plant height all are higher than control group.Wherein experimental group 2 cultivation effects are the most obvious, and the survival rate of Damask Rose seedling is up to 84.6%, and the plant average height is up to 25.2cm, and seedling growth potential is best, and blade is dark green, and cane is more sturdy.
Claims (1)
1. method for transplanting Damascus rose tissue culture seedling is characterized in that, after one week,, directly transplant on the cave dish that transplanting medium is housed root medium wash clean with clear water by taking-up group training seedling from blake bottle in the greenhouse hardening for the Damascus rose tissue culture seedling seedling; Water one time 0.1% the carbendazim aqueous solution after the field planting, watered once in 5 days again at every interval, waters continuously 3 times, and film is added a cover in the seedbed, 20～23 ℃ of cultivation temperature, humidity 90%～95%, shading 60～70%, illumination 6000～8000lx; Once dilute 10 times MS liquid nutrient medium every pouring in 3 days, promote the sprouting of root growth and leaf; After young leaves was extracted out, humidity was reduced to 70～80%, and illumination is enhanced to 8000～12000lx, and cultivation temperature is 23～28 ℃; Transplanting medium is peat, river sand, perlitic mixed-matrix, and its volume ratio is 3～4: 2～3: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105375585A CN102090329B (en) | 2010-11-10 | 2010-11-10 | Method for transplanting Damascus rose tissue culture seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105375585A CN102090329B (en) | 2010-11-10 | 2010-11-10 | Method for transplanting Damascus rose tissue culture seedling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102090329A CN102090329A (en) | 2011-06-15 |
| CN102090329B true CN102090329B (en) | 2013-02-20 |
Family
ID=44123731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105375585A Active CN102090329B (en) | 2010-11-10 | 2010-11-10 | Method for transplanting Damascus rose tissue culture seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102090329B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108464207A (en) * | 2018-04-13 | 2018-08-31 | 中国水稻研究所 | A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate |
| CN111011190A (en) * | 2019-12-20 | 2020-04-17 | 云南省农业科学院花卉研究所 | Method for transplanting and transiting tissue culture seedlings of insect-eating plants |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535253A (en) * | 2013-10-23 | 2014-01-29 | 镇江市丹徒区紫杉生态农业园 | Cultivating method of rose nutrient solution |
| CN104429925B (en) * | 2014-11-06 | 2018-09-28 | 王明琴 | A kind of rose and its breeding method |
| CN104584835B (en) * | 2015-01-27 | 2017-06-16 | 福建省农业科学院作物研究所 | A kind of rose growing on hill-side method |
| CN106818120A (en) * | 2016-12-24 | 2017-06-13 | 庆阳敦博科技发展有限公司 | A kind of method for transplanting of bitter water rose tissue-cultured seedling |
| CN107493908A (en) * | 2017-09-06 | 2017-12-22 | 莫斌 | A kind of Damask Rose epicormic branch cutting method |
| CN109089814A (en) * | 2018-06-14 | 2018-12-28 | 云南丰德生物科技有限公司 | A kind of rose cuttage breeding method |
| CN110115221A (en) * | 2019-06-19 | 2019-08-13 | 陆彤 | A kind of matrix and its preparation method for breeding Damask Rose seedling |
-
2010
- 2010-11-10 CN CN2010105375585A patent/CN102090329B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108464207A (en) * | 2018-04-13 | 2018-08-31 | 中国水稻研究所 | A kind of cultural method improving rice tissue-cultured seedling transplanting survival rate |
| CN111011190A (en) * | 2019-12-20 | 2020-04-17 | 云南省农业科学院花卉研究所 | Method for transplanting and transiting tissue culture seedlings of insect-eating plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102090329A (en) | 2011-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102090329B (en) | Method for transplanting Damascus rose tissue culture seedling | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN101822220A (en) | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii | |
| CN103749302A (en) | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings | |
| CN101889547A (en) | Aseptic and rapid propagation method of dendrobium devonianum seeds | |
| CN103734009B (en) | Tissue culture method of anthurium | |
| CN101836585B (en) | Tissue-culture seedling raising method of rhodiola crenulata | |
| CN103931497A (en) | Method for improving seedling rate of tissue culture seedlings of hylocereus undulatus britt | |
| CN102884922A (en) | Method for improving cuttage survival rate of holly | |
| CN102090337A (en) | Rapid propagation method of rhododendron latoucheae | |
| CN104041412A (en) | Rapid propagation method for tissue culture of Guizhou hemiboea cavaleriei | |
| CN104012417A (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN103430845A (en) | Strawberry tissue culturing method | |
| CN103460971B (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN105638220A (en) | Cutting propagation method for magnolia soulangeana | |
| CN102823502A (en) | Method for intermediately propagating and culturing vitis quinquangularis in vitro | |
| CN101352146A (en) | Quick breeding method for tissue culture of Geraldton wax | |
| CN101637123B (en) | Organic-culturing quick-reproducing method of curcuma | |
| CN103125393A (en) | Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am | |
| CN105104143A (en) | Water planting cutting propagation method for myrtus communis | |
| CN104770173A (en) | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings | |
| CN104542284A (en) | Tissue culture rapid propagation method for rhododendron irroratum | |
| CN105494097A (en) | In-vitro rapid propagation technology of viburnum sargentii koehne | |
| CN105010142A (en) | Vietnamese Aquilaria agallocha Roxb tissue culture method | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 300300 Dongli District, Tianjin Huan Huan Road, No. 6901 Patentee after: DASHUN INTERNATIONAL FLOWER CO., LTD. Address before: 300300 Dongli District, Tianjin Huan Huan Road, No. 6901 Patentee before: Tianjin Binhai International Flower Technology Park Co., Ltd. |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for transplanting Damascus rose tissue culture seedling Effective date of registration: 20190219 Granted publication date: 20130220 Pledgee: Tianjin Caixin Huitong small loan Co., Ltd. Pledgor: DASHUN INTERNATIONAL FLOWER CO., LTD. Registration number: 2019120000007 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200817 Granted publication date: 20130220 Pledgee: Tianjin Caixin Huitong small loan Co.,Ltd. Pledgor: DASHUN INTERNATIONAL FLOWER Co.,Ltd. Registration number: 2019120000007 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |