CN111011190A - Method for transplanting and transiting tissue culture seedlings of insect-eating plants - Google Patents
Method for transplanting and transiting tissue culture seedlings of insect-eating plants Download PDFInfo
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- CN111011190A CN111011190A CN201911325708.3A CN201911325708A CN111011190A CN 111011190 A CN111011190 A CN 111011190A CN 201911325708 A CN201911325708 A CN 201911325708A CN 111011190 A CN111011190 A CN 111011190A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to a method for transplanting transition of tissue culture seedlings of insect-eating plants, belonging to the technical field of transplanting of tissue culture seedlings of insect-eating plants, which takes insect-eating plant tissue culture seedlings as transplanting objects and transplants the seedlings by the following modes: washing the roots of the rooted seedlings and soaking the roots in 1000-1250 ppm thiophanate methyl or carbendazim for 10-15 min; selecting a mixture of perlite and turf with a volume ratio of 1.5-2:1 as a culture medium, and culturing for 15-20 days in a shading way of 78-80%; shading 30-40% of the plant, and culturing for 15-20 days; and (5) counting the survival rate after 10 days of full-light cultivation. The invention can directly transplant the plant without hardening the seedling after cleaning the culture medium, and can achieve higher transplanting survival rate through the control management in the transplanting process.
Description
Technical Field
The invention belongs to the technical field of insect-eating plant seedling culture and transplantation, and particularly relates to a method for insect-eating plant tissue culture seedling transplantation transition.
Background
An insect-feeding plant is an autotrophic plant that can capture and digest animals to obtain nutrition (non-energy) using certain parts of the plant body (most of the insect-catching organs are metamorphic leaves). Growing in soil-barren, particularly nitrogen-deficient, areas such as acid swamps and stony desertification. Most prey of insect-feeding plants are insects and arthropods. These insect-eating plants, which are capable of attracting and capturing prey, producing digestive enzymes and absorbing nutrients, are distributed globally, but as their living environment is destroyed, a certain number of insect-eating plants have been or are nearing extinction at present. The insect-eating plant has quite good ornamental value in the flower and garden world due to the novel appearance and the unique special morphological structure of the insect-eating plant, and is a plant with great economic value. Tissue culture and in-vitro rapid propagation of the insect-eating plant can culture a large number of high-quality seedlings which grow robustly and uniformly in a short time, can effectively save deficient germplasm resources and fill in the vacancy of market supply. At present, the tissue culture and in vitro propagation of pitcher plant and sundew in five major insect-eating plants are mature day by day. However, the tissue culture rooted seedlings of the pitcher plant are transplanted after hardening the seedlings for 18 to 25 days; the sundew tissue culture rooted seedlings are sealed and hardened for 5-7d, then are transplanted after being opened and hardened for 2-4d, and the survival rate of insect-eating plants which are not hardened is lower after being transplanted.
In the hardening-seedling period, the growth of the plants is slowed, and the plants are directly transplanted without hardening-seedling, so that the survival rate is low. In the hardening period of the seedlings, management such as air release, cooling, water control and the like is needed, the workload is large, the implementation conditions are harsh, and much labor is consumed.
Disclosure of Invention
In order to overcome the problems in the background art, the invention provides a method for the transfer of the tissue culture seedlings of the insect-eating plants in the transplanting process, which does not need to acclimatize the seedlings, directly transplants the seedlings after cleaning the culture medium, and can achieve higher transplanting survival rate through the control management in the transplanting process.
In order to realize the purpose, the invention is realized by the following technical scheme:
the method for transplanting and transiting the tissue culture seedlings of the insect-eating plants comprises the following steps:
(1) after washing the roots of the tissue culture seedlings, soaking the roots of the tissue culture seedlings in 1000-1250 ppm thiophanate methyl or carbendazim for 10-15 min;
(2) shading 70-80% of the seedlings in the step (1) for cultivation (cultivation stage I);
(3) shading 30-40% of the seedlings in the step (2) for cultivation (cultivation stage II);
the substrate used for cultivation is a mixture of perlite and turf in a volume ratio of 1.5-2:1, and is uniformly mixed with 1500-2000 ppm of mancozeb or chlorothalonil solution before use;
further, the turf is non-fertilizer turf with the particle size of 4-6 mm; the perlite has a particle size of 3-7 mm.
Further, the step (2) and the step (3) are cultivated in a stuffy box; the top cover of the stuffy culture seedling box is a transparent cover, and the top cover is provided with a humidity adjusting hole which can be opened and closed.
Furthermore, perlite needs to be paved at the bottom of the culture medium.
Further, the cultivation time is 15-20 days, wherein the cultivation time is 70-80% in shade.
Further, the cultivation time is 15-20 days with 30-40% shading.
Drawings
FIG. 1: example 1 physical diagram of sundew cultivation process;
FIG. 2: example 2 physical picture of pitcher plant cultivation process;
FIG. 3: example 2 physical picture after transplanting nepenthes for 20 days.
The invention has the beneficial effects that:
according to the method for transiting transplanting of the tissue culture seedlings of the insect-eating plants, seedling hardening is not needed, the seedlings are directly transplanted after the culture medium is cleaned, and the transplanting survival rate can be higher through control management in the transplanting process.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding of the skilled person.
Example 1
In the invention, the best suitable time for transplanting the insect-eating plants is 2-10 months per year, the sundew root-taking seedlings cultured in a culture room for 20 days are transferred to a greenhouse, the seedlings are taken out of a culture bottle in a shady and sunnless direct-irradiation place, a culture medium is shaken clean, the rest culture medium is washed clean under running water, the seedlings are soaked in 1000-1250 ppm thiophanate methyl or carbendazim for 10min, the seedlings are taken out and placed in a perforated sieve, and the seedlings are covered by wet cloth.
The culture medium adopts perlite: mixing the grass peat and the grass peat; the turf is the non-fertilizer turf with the particle size of 5mm, can enhance the water retention capacity of the matrix and is good for the later growth of the root system; the perlite has a particle size of about 5mm, and can improve the porosity of the matrix and enhance the air permeability of the matrix.
Before the culture medium is used, the culture medium is uniformly mixed with 1500-2000 ppm mancozeb or chlorothalonil solution, and the culture medium can be kneaded by hands to form a cluster and does not produce water. Before filling the matrix, about 1cm of perlite should be filled at the bottom of each small hole of the plug tray to facilitate ventilation.
Before transplanting, the hole tray filled with the matrix (a trimmed 72-hole PS hole tray, the length, the width and the height are 51cm multiplied by 25cm multiplied by 4.5cm, and the specification of small holes is 4.5cm multiplied by 4.5cm) is placed in a tray of a closed seedling raising box (the closed seedling raising box comprises the tray and a top cover, the tray is a hard solid tray made of black ABS resin, the external specification is 54cm multiplied by 28cm multiplied by 6.5cm, the internal specification is 52cm multiplied by 26cm multiplied by 5.5cm, the top cover is a PVC transparent cover of 54cm multiplied by 28cm multiplied by 18cm, the left side and the right side of the upper part of the top cover are respectively provided with 4 square distributed small holes with the diameter of 1cm, and the excellent small hole with the diameter of 0.5cm at the center of the square is used for buckling a top buckle rotating button so as to adjust the humidity in the seedling raising box).
Hold the sundew root portion root with sharp tweezers, insert perpendicularly in the cave hole, the depth of insertion uses the root to submerge the matrix completely as the standard, and whole process goes on under the little hunch canopy that covers the sunshade net, has planted a dish, sprays water immediately, covers the transparent PVC top cap of vexed fostering seedling case, screws the top knob.
And (3) cultivation stage I: after culturing for several days under different shading rates, unscrewing a rotating button at the top of the PVC top cover to expose the small hole; and (3) cultivation stage II: changing the sunshade net to adjust the refractive index, adding water from the edge of the tray every 10 days by a pot soaking method to keep the humidity in the seedling box, uncovering the sunshade net after culturing for a plurality of days under the condition, and counting the survival rate after 10 days. After the transplanting, the invention only needs to add water once every 10 days, and does not need to invest excessive manpower and management cost.
The cultivation period I and II, the shading rate, the cultivation days and the survival rate after transplanting are shown in the following table.
Example 2
In the invention, the best suitable time for transplanting the insect-eating plants is 2-10 months per year, the nepenthes rooting seedlings cultured in a culture room for 30 days are transferred to a greenhouse, the seedlings are taken out of a culture bottle in a shady and sunnless direct-irradiation place, a culture medium is shaken clean, the rest culture medium is washed clean under running water, the seedlings are soaked in 1000-1250 ppm thiophanate methyl or carbendazim for 10min, the seedlings are taken out and placed in a perforated sieve, and the seedlings are covered with wet cloth.
Perlite adopted by the culture medium: mixing the grass peat and the grass peat; the turf is the non-fertilizer turf with the particle size of 5mm, can enhance the water retention capacity of the matrix and is good for the later growth of the root system; the perlite has a particle size of about 5mm, and can improve the porosity of the matrix and enhance the air permeability of the matrix.
Before the culture medium is used, the culture medium is uniformly mixed with 1500-2000 ppm mancozeb or chlorothalonil solution, and the culture medium can be kneaded by hands to form a cluster and does not produce water. Before filling the matrix, about 1cm of perlite should be filled at the bottom of each small hole of the plug tray to facilitate ventilation.
Before transplanting, the hole tray filled with the matrix (a trimmed 72-hole PS hole tray, the length, the width and the height are 51cm multiplied by 25cm multiplied by 4.5cm, and the specification of small holes is 4.5cm multiplied by 4.5cm) is placed in a tray of a closed seedling raising box (the closed seedling raising box comprises the tray and a top cover, the tray is a hard solid tray made of black ABS resin, the external specification is 54cm multiplied by 28cm multiplied by 6.5cm, the internal specification is 52cm multiplied by 26cm multiplied by 5.5cm, the top cover is a PVC transparent cover of 54cm multiplied by 28cm multiplied by 18cm, the left side and the right side of the upper part of the top cover are respectively provided with 4 square distributed small holes with the diameter of 1cm, and the excellent small hole with the diameter of 0.5cm at the center of the square is used for buckling a top buckle rotating button so as to adjust the humidity in the seedling raising box).
The root of the basic part of the pitcher plant is pinched by the sharp forceps and is vertically inserted into the hole, the insertion depth is based on the fact that the root is completely immersed into the matrix, the whole process is carried out under a small arched shed covering the sunshade net, a disc is planted, water is sprayed immediately, a transparent PVC top cover is covered, and a top rotating button is screwed.
And (3) cultivation stage I: after culturing for several days under different shading rates, unscrewing a rotating button at the top of the PVC top cover to expose the small hole; and (3) cultivation stage II: changing the sunshade net to adjust shading rate, adding water from the edge of the tray every 10 days by a pot soaking method to maintain the humidity in the seedling box, culturing for several days under the condition, then uncovering the sunshade net, and counting the survival rate after 10 days. After the transplanting, the invention only needs to add water once every 10 days, and does not need to invest excessive manpower and management cost.
The cultivation period I and II, the shading rate, the cultivation days and the survival rate after transplanting are shown in the following table.
Comparative example:
the best suitable time for transplanting the insect-eating plants is 2-10 months per year, the sundew root seedlings cultured for 20 days in a culture room are transferred to a greenhouse, the seedlings are taken out of a culture bottle in a shady and sunny-free direct-irradiation place, the culture medium is shaken clean, the rest culture medium is washed clean under running water, the seedlings are soaked in 1000-1250 ppm thiophanate methyl or carbendazim for 10min, the seedlings are taken out and put into a perforated sieve, and the sieve is covered with wet cloth.
Before the culture medium is used, the culture medium is uniformly mixed with 1500-2000 ppm mancozeb or chlorothalonil solution, and the culture medium can be kneaded by hands to form a cluster and does not produce water. Before filling the matrix, about 1cm of perlite should be filled at the bottom of each small hole of the plug tray to facilitate ventilation.
The culture medium adopts perlite with a volume ratio of 2: 1: mixing the grass peat and the grass peat; the turf is the non-fertilizer turf with the particle size of 5mm, can enhance the water retention capacity of the matrix and is good for the later growth of the root system; the perlite has a particle size of about 5mm, and can improve the porosity of the matrix and enhance the air permeability of the matrix.
Directly planting the sundew tissue culture seedlings into a hole tray, and placing under a small arched shed covered with a plastic film for cultivation. In the period I of cultivation, water is sprayed 1 time every 3 days to increase humidity. In the second cultivation period, the sunshade net is removed, the plastic films at two ends of the small arched shed are gradually removed after being uncovered and ventilated for 3-5 days, water is sprayed every day (3-5 times in summer and 2-3 times in spring and autumn), and the survival rate is counted after 20 days.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (6)
1. A method for transplanting and transiting tissue culture seedlings of insect-eating plants is characterized by comprising the following steps: the method for transplanting and transiting the tissue culture seedlings of the insect-eating plants comprises the following steps:
after washing the roots of the tissue culture seedlings, soaking the roots of the tissue culture seedlings in 1000-1250 ppm thiophanate methyl or carbendazim for 10-15 min;
shading 70-80% of the seedlings in the step (1) for cultivation;
shading 30-40% of the seedlings in the step (2) for cultivation;
the substrate used for cultivation is a mixture of perlite and turf in a volume ratio of 1.5-2:1, and is uniformly mixed with 1500-2000 ppm of mancozeb or chlorothalonil solution before use.
2. The method for transplanting transition of tissue culture seedlings of insect-eating plants according to claim 1, which is characterized in that: the turf is non-fertilizer turf with the particle size of 4-6 mm; the perlite has a particle size of 3-7 mm.
3. The method for transplanting transition of tissue culture seedlings of insect-eating plants according to claim 2, which is characterized in that: cultivating in a closed seedling cultivating box in the step (2) and the step (3); the top cover of the stuffy culture seedling box is a transparent cover, and the top cover is provided with a humidity adjusting hole which can be opened and closed.
4. The method for transiting transplanting of tissue culture seedlings of insect plants according to claim 1, wherein: the bottom of the culture medium needs to be paved with perlite.
5. The method for transplanting transition of tissue culture seedlings of insect-eating plants according to claim 1, which is characterized in that: shading 70-80% of the cultivation time is 15-20 days.
6. The method for transplanting transition of tissue culture seedlings of insect-eating plants according to claim 1, which is characterized in that: shading 30-40% of the cultivation time is 15-20 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113100066A (en) * | 2021-05-14 | 2021-07-13 | 中国热带农业科学院环境与植物保护研究所 | Efficient transplanting method for litchi tissue culture seedlings |
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