CN101049091A - Transplanting method for breeding grouped seedling of fleshiness plant - Google Patents
Transplanting method for breeding grouped seedling of fleshiness plant Download PDFInfo
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- CN101049091A CN101049091A CNA2007100685704A CN200710068570A CN101049091A CN 101049091 A CN101049091 A CN 101049091A CN A2007100685704 A CNA2007100685704 A CN A2007100685704A CN 200710068570 A CN200710068570 A CN 200710068570A CN 101049091 A CN101049091 A CN 101049091A
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Abstract
A transplanting method for the tissue-cultured plantlet of pulpy plant includes such steps as taking the tissue-cultured plantlet out of the culture bottle, water washing for removing culture medium, dipping in the solution of wettable powder of carbendazim, drying in the air, transplanting it in the culture matrix, covering film for 7-10 days but opening the film for 1 hr in the morning and at night, and conventional transplanting and managing.
Description
Technical field
The present invention relates to a kind of technology of plant tissue culture transplantation of seedlings, mainly is a kind of transplanting method of breeding grouped seedling of fleshiness plant.
Background technology
Utilizing Plant Tissue Breeding to carry out quick propagating technology is the plant seedling means of production commonly used during flower gardening is produced, and it mainly is divided into four production stages, i.e. the foundation of aseptic seedling, enrichment culture, culture of rootage, tissue cultivating seedling are transplanted.Wherein tissue cultivating seedling is transplanted and to be one and to become autotrophy by the plant heterotrophism, by aseptic becoming bacterium is arranged, by constant temperature, high humidity, the low light level gradually to alternating temperature, low humidity, the process of high light transition, specification requirement is very high, careless slightly, will reduce transplanting survival rate, thereby increase the seedling production cost.Conventional at present transplanting method is that the tissue cultivating seedling bottle outlet is cleaned, directly plant to adopt behind the cultivation matrix and shelter from heat or light more than 60%, epiphragma keeps air humidity more than 90%, and temperature is controlled at 20～24 ℃, increase illumination after 7～10 days gradually and reduce air humidity, make seedling adapt to external condition gradually.
Succulent is very popular in recent years a kind of ornamental plants; relate to Cactaceae; Liliaceae; Aizoaceae; Crassulaceae; Euphorbiaceae; several ten thousand plant species such as composite family; its breeding is mainly by sowing and cuttage; but this can not satisfy the demand in market; therefore breed now by plant tissue culture technique; but breeding grouped seedling of fleshiness plant is constant relatively in temperature; high humidity; the environment of closed sterile soft is growth down; its epidermis protective tissue does not have substantially; interior confluent monolayer cells contains the moisture content more than 90%, and is when therefore transplanting method routinely is transplanted to tissue cultivating seedling in the cultivation matrix, changeable in ambient temperature; germ is assorted to be given birth to; be easy to rot under the environment of high humidity, transplanting survival rate is not high, has only about 30%.
Summary of the invention
The present invention will solve the defective of above-mentioned prior art, and a kind of transplanting method of breeding grouped seedling of fleshiness plant is provided.
The present invention solves the technical scheme that its technical problem adopts.The transplanting method of this breeding grouped seedling of fleshiness plant, carry out as follows:
1), tissue cultivating seedling bottle outlet: breeding grouped seedling of fleshiness plant is taken out from blake bottle carefully, clean the medium of tissue cultivating seedling base portion with running water;
2), tissue cultivating seedling sterilization: tissue cultivating seedling is dipped in the 50% carbendazol wettable powder solution of 500mg/L;
3), tissue cultivating seedling dries: placing the ventilating and cooling place then, drying to the tissue cultivating seedling surface has shrinkage slightly;
4), tissue cultivating seedling is transplanted: tissue cultivating seedling is planted in the cultivation matrix, and epiphragma keeps humidity more than 90%, and is sooner or later respectively ventilating 1 hour;
5), tissue cultivating seedling management: can manage according to the tissue cultivating seedling transplanting method of routine after 7～10 days.
Tissue cultivating seedling flash-off time of the present invention needs 2～3 days.
The cultivation matrix that the present invention adopts is peat and perlitic mixed-matrix, and peat and perlitic volume ratio are 2: 1.
The effect that the present invention is useful is: the lack of water shrinkage of fleshiness tissue cultivating seedling surface is scabbed, increased resistance, improved the transplanting survival rate of fleshiness tissue cultivating seedling greatly, and seedling-slowing stage short, take root soon, can take root in 7～10 days, after taking root, well developed root system, plant strain growth is fast, and the plant plumpness is full, and is manageable.The inventive method is simple, and processing ease does not increase cost, and transplanting survival rate is brought up to more than 98%.
Embodiment
The invention will be described further below in conjunction with embodiment: but content of the present invention is not limited thereto.
The method that embodiment 1 this breeding grouped seedling of fleshiness plant is transplanted, concrete steps are as follows:
1), tissue cultivating seedling bottle outlet: breeding grouped seedling of fleshiness plant is taken out from blake bottle carefully, clean the medium of tissue cultivating seedling root with running water;
2), tissue cultivating seedling sterilization: tissue cultivating seedling is dipped in the 50% carbendazol wettable powder solution of 500mg/L;
3), tissue cultivating seedling dries: fitly tissue cultivating seedling is put in clean tray, be placed on the ventilating and cooling place 2～3 days, drying to the tissue cultivating seedling surface has shrinkage slightly;
4), tissue cultivating seedling is transplanted: tissue cultivating seedling is planted in the moistening cultivation matrix, cultivation matrix is peat and perlitic mixed-matrix, and peat and perlitic volume ratio are 2: 1, transplants the back epiphragma and keeps humidity more than 90%, and sooner or later respectively ventilating the cooling of to shelter from heat or light summer 1 hour;
5), tissue cultivating seedling management: can manage according to the tissue cultivating seedling transplanting method of routine after 7～10 days, increase ventilation time gradually, complete until root growth.
The method that embodiment 2 this breeding grouped seedling of fleshiness plant are transplanted, concrete steps are as follows:
1), tissue cultivating seedling bottle outlet: 12 volume soft leaf class longevity of genus of succulent, precious careless tissue cultivating seedling are taken out from blake bottle carefully, clean the medium of tissue cultivating seedling root with running water;
2), tissue cultivating seedling sterilization: tissue cultivating seedling is dipped in the 50% carbendazol wettable powder solution of 500mg/L;
3), tissue cultivating seedling dries: fitly tissue cultivating seedling is put in clean tray, be placed on the ventilating and cooling place 3 days, drying to the tissue cultivating seedling surface has shrinkage slightly;
4), tissue cultivating seedling is transplanted: tissue cultivating seedling is planted in the moistening cultivation matrix, cultivation matrix is peat and perlitic mixed-matrix, and peat and perlitic volume ratio are 2: 1, transplants the back epiphragma and keeps humidity more than 90%, and sooner or later respectively ventilating the cooling of to shelter from heat or light summer 1 hour;
5), tissue cultivating seedling management: can manage according to the tissue cultivating seedling transplanting method of routine after 7～10 days, increase ventilation time gradually, complete until root growth.
The method that embodiment 3 this breeding grouped seedling of fleshiness plant are transplanted, concrete steps are as follows:
1), tissue cultivating seedling bottle outlet: succulent 12 volume genus sclerophyll class stripeds 12 volumes, beautiful 12 volume tissue cultivating seedling are taken out from blake bottle carefully, clean the medium of tissue cultivating seedling root with running water;
2), tissue cultivating seedling sterilization: tissue cultivating seedling is dipped in the 50% carbendazol wettable powder solution of 500mg/L;
3), tissue cultivating seedling dries: fitly tissue cultivating seedling is put in clean tray, be placed on the ventilating and cooling place 2 days, drying to the tissue cultivating seedling surface has shrinkage slightly;
4), tissue cultivating seedling is transplanted: tissue cultivating seedling is planted in the moistening cultivation matrix, cultivation matrix is peat and perlitic mixed-matrix, and peat and perlitic volume ratio are 2: 1, transplants the back epiphragma and keeps humidity more than 90%, and sooner or later respectively ventilating the cooling of to shelter from heat or light summer 1 hour;
5), tissue cultivating seedling management: can manage according to the tissue cultivating seedling transplanting method of routine after 7～10 days, increase ventilation time gradually, complete until root growth.
Claims (3)
1, a kind of transplanting method of breeding grouped seedling of fleshiness plant is characterized in that: its step is as follows:
1.1), the tissue cultivating seedling bottle outlet: breeding grouped seedling of fleshiness plant is taken out from blake bottle, cleans the medium of tissue cultivating seedling base portion with running water;
1.2), the tissue cultivating seedling sterilization: tissue cultivating seedling is dipped in the 50% carbendazol wettable powder solution of 500mg/L;
1.3), tissue cultivating seedling dries: placing the ventilating and cooling place then, drying to the tissue cultivating seedling surface has shrinkage slightly;
1.4), tissue cultivating seedling transplants: tissue cultivating seedling is planted in the cultivation matrix, and epiphragma keeps humidity more than 90%, and is sooner or later respectively ventilating 1 hour;
1.5), tissue cultivating seedling management: can manage according to the tissue cultivating seedling transplanting method of routine after 7～10 days.
2, the transplanting method of breeding grouped seedling of fleshiness plant according to claim 1 is characterized in that, described tissue cultivating seedling flash-off time needs 2～3 days.
3, the transplanting method of breeding grouped seedling of fleshiness plant according to claim 1 is characterized in that, described cultivation matrix is peat and perlitic mixed-matrix, and peat and perlitic volume ratio are 2: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100685704A CN101049091A (en) | 2007-05-14 | 2007-05-14 | Transplanting method for breeding grouped seedling of fleshiness plant |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100685704A CN101049091A (en) | 2007-05-14 | 2007-05-14 | Transplanting method for breeding grouped seedling of fleshiness plant |
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| CNA2007100685704A Pending CN101049091A (en) | 2007-05-14 | 2007-05-14 | Transplanting method for breeding grouped seedling of fleshiness plant |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499090A (en) * | 2011-11-07 | 2012-06-20 | 上海旭东园艺有限公司 | Method for isolated culture of Haworthia succulent plants |
| CN103141387A (en) * | 2013-03-08 | 2013-06-12 | 浙江省农业科学院 | Method for cultivating haworthia maughanii tissue |
| CN103270947A (en) * | 2013-03-08 | 2013-09-04 | 浙江省农业科学院 | Duvalia angustiloba tissue culturing method |
| CN106258397A (en) * | 2016-08-12 | 2017-01-04 | 广西鑫雅皇庭园林工程有限责任公司 | A kind of implantation methods of Bryophyllum succulent |
| CN107960297A (en) * | 2017-11-10 | 2018-04-27 | 贵溪欧绿多肉植物有限公司 | The cultivation implantation methods of succulent |
| CN111011190A (en) * | 2019-12-20 | 2020-04-17 | 云南省农业科学院花卉研究所 | Method for transplanting and transiting tissue culture seedlings of insect-eating plants |
-
2007
- 2007-05-14 CN CNA2007100685704A patent/CN101049091A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499090A (en) * | 2011-11-07 | 2012-06-20 | 上海旭东园艺有限公司 | Method for isolated culture of Haworthia succulent plants |
| CN103141387A (en) * | 2013-03-08 | 2013-06-12 | 浙江省农业科学院 | Method for cultivating haworthia maughanii tissue |
| CN103270947A (en) * | 2013-03-08 | 2013-09-04 | 浙江省农业科学院 | Duvalia angustiloba tissue culturing method |
| CN103270947B (en) * | 2013-03-08 | 2014-09-03 | 浙江省农业科学院 | Duvalia angustiloba tissue culturing method |
| CN106258397A (en) * | 2016-08-12 | 2017-01-04 | 广西鑫雅皇庭园林工程有限责任公司 | A kind of implantation methods of Bryophyllum succulent |
| CN107960297A (en) * | 2017-11-10 | 2018-04-27 | 贵溪欧绿多肉植物有限公司 | The cultivation implantation methods of succulent |
| CN111011190A (en) * | 2019-12-20 | 2020-04-17 | 云南省农业科学院花卉研究所 | Method for transplanting and transiting tissue culture seedlings of insect-eating plants |
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Open date: 20071010 |