CN1742542A - Method for directly taking root of tea-tree tissue culture seedling - Google Patents
Method for directly taking root of tea-tree tissue culture seedling Download PDFInfo
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- CN1742542A CN1742542A CN 200510060923 CN200510060923A CN1742542A CN 1742542 A CN1742542 A CN 1742542A CN 200510060923 CN200510060923 CN 200510060923 CN 200510060923 A CN200510060923 A CN 200510060923A CN 1742542 A CN1742542 A CN 1742542A
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Abstract
The invention discloses a kind of method for directly taking root of tea-tree tissue culture seedling, can improve the survival rate of tea-tree tissue culture seedling, simplify the operation sequence of taking root, shorten cultivation cycle.Realize by following steps: be experiment material with the tea-tree tissue culture seedling, handle with certain density HORMONE TREATMENT, the survival rate of tea-tree tissue culture seedling reaches more than 70% after 60 days, and the rooting rate that survives seedling reaches 90%, and mean elements and root length reach 9.4 and 3.64CM respectively.The inventive method (comparing with in vitro taking root) has been simplified the rooting method of tea-tree tissue culture seedling, has shortened tea-tree tissue culture seedling and has adapted to 30 days cycles.The present invention paves the way for the application of tissue culture technology on tea tree is grown seedlings, and helps the quick popularization and the breeding of new tea kind.
Description
Technical field
The invention belongs to seedling-cultivating method, relate generally to method for directly taking root of tea-tree tissue culture seedling, specifically relate to tissue cultivating seedling and pass through hormone induction rooting method rapidly and efficiently in the greenhouse.
Background technology
The reproduction coefficient of tea tree single node cutting is about 1: 50～and 60, to compare gap very far away with field crop (more than 1: 1000).Because reproduction coefficient is low, after the new tea kind is bred, generally need the protospecies breeding time in about 10 years, just can reach the ability of growing seedlings of certain scale, the species the advantage of new varieties then needs the longer time to embody aborning, so the cultivation of new tea kind and promotion rate are very slow.Utilize the tissue culture technology can the fast breeding seedling, improve reproduction coefficient, but tissue culture technology also fails really to use growing seedlings up of tea tree at present, one of them most important factor lacks the technology of taking root fast and effectively exactly.Tea-tree tissue culture seedling often all adopts in vitro to take root for transplanting then to matrix and carries out cultured method at present.And the present rooting rate of rooting method in vitro generally has only about 60%, the cycle of taking root is about 60 days, because the root of tissue cultivating seedling formation in vitro is the tender fleshy root of children, after in being transplanted to matrix, greatly since root to the inadaptability of matrix and death, the survival rate that causes transplanting is less than 50%, cause the huge waste of resource, make the application of tissue culture technology on tea tree is grown seedlings obtain restriction, and at present owing to improve the needs of tea industry, the problem of tea place improved variety need to be resolved hurrily.The problem that solves improved variety then is the quick breeding by group culture of new varieties nursery stock, and the technology of taking root becomes the bottleneck that tissue culture technology is used.Therefore the rooting rate that improves tissue cultivating seedling is the problem that presses for solution at present.
Summary of the invention
The purpose of this invention is to provide a kind of method for directly taking root of tea-tree tissue culture seedling, is by improving existing tea-tree tissue culture seedling rooting method, proposing to be fit to the fast direct rooting method of production application.
The present invention implements by following steps:
(1) hardening is handled before the tea-tree tissue culture seedling rooting: test material is a tea-tree tissue culture seedling, and the explant source is axillalry bud, and kind is a Dragon Well tea 43.Choose highly for about 5-6cm, the more consistent group training seedling of growing state, be transferred in the MS medium about 10 days strong sprouts, preceding 3 days of test bottle is opened, be put in the greenhouse and practiced seedling 3 days;
(2) the tissue cultivating seedling processing of taking root: adopt different root-promoting hormone and variable concentrations, and adopt different dipping the processing time, after the tissue cultivating seedling base portion was handled, directly cuttage went in the matrix to take root;
(3) the take root investigation of situation:, the situation of taking root (rooting rate, lethality, radical and root are long) of tea-tree tissue culture seedling is carried out counting statistics, the relatively situation of the taking root difference between different disposal since the 20th day.
The environment of taking root of tissue cultivating seedling is: 25 ℃～35 ℃ of temperature, and humidity 60%～90%, used transplanting medium is a peat: perlite: vermiculite=2: 1: 1 (volume ratio).
The beneficial effect of method for directly taking root of tea-tree tissue culture seedling provided by the invention has following several respects: the firstth, and the transplantation rooting survival rate of the tea-tree tissue culture seedling that improves has solved the problem of tea-tree tissue culture seedling transplantation rooting difficulty.The present invention has screened the hormone kind of the direct root induction in suitable tea-tree tissue culture seedling greenhouse, concentration, and the processing time etc., thereby set up the tea-tree tissue culture seedling greenhouse technology of directly taking root, compare with existing rooting method in vitro, it can be brought up to the survival rooting rate of tissue cultivating seedling more than 60% and take root all rightly, and mean elements and root be long to reach 9.4 and 3.64CM respectively.Second has shortened about the 30 days hardening time of tea shoot growing fine after tissue cultivating seedling is taken root.The present invention paves the way for the application of tissue culture technology on tea tree is grown seedlings, and helps the quick popularization and the breeding of new tea kind.
Embodiment
The present invention is described further by embodiment.
Embodiment one
With taking root of Dragon Well tea 43 tissue cultivating seedling is example, and directly the specific descriptions of rooting method are as follows:
1. the acquisition of tea-tree tissue culture transplanted seedling
(1) test material is a tea-tree tissue culture seedling, and the explant source is axillalry bud.Choose highly for about 5-6cm, the more consistent group training seedling of growing state, be transferred in the MS medium about 10 days strong sprouts, preceding 3 days of test bottle is opened, be put in the greenhouse hardening 3 days;
(2) tissue cultivating seedling is taken out from blake bottle, clean the medium of bottom, and the brown callus that base portion produces is removed, use the carbendazim solution rinsing.
2. the processing of taking root of tea-tree tissue culture seedling
Adopt certain density HORMONE TREATMENT tea-tree tissue culture seedling base portion, in the matrix that its cuttage is gone into to be mixed, the percent by volume of matrix is a peat: perlite: vermiculite=2: 1: 1 then.
3. take root the environment control and the management of tissue cultivating seedling
(1) temperature control: adopt the cooling effect of sunshade net and switch, axial flow blower and the water Lian in skylight and the realization of hot blast warming-up device to temperature controlling, the growing environment temperature of tea-tree tissue culture seedling is controlled between 25 ℃～35 ℃;
(2) humidity control: adopt spraying system that the air humidity in the tissue cultured seedling growth environment is adjusted, make it be controlled at 60%～90%;
(3) intensity of illumination: about tissue cultivating seedling transplanting initial stage (30) sky,, prevent that tissue cultivating seedling is too strong dead because of illumination to its processing of sheltering from heat or light of carrying out 70%.Carry out full exposure after 30 days.
4. the final-period management of tissue cultivating seedling
Tissue cultivating seedling is after taking root, and it is consistent with cuttage seeding with environmental management to apply fertilizer.
Embodiment two:
With Dragon Well tea the taking root of tissue cultivating seedling of coming into leaves is example, and directly the specific descriptions of rooting method are as follows:
(1) acquisition of tea-tree tissue culture transplanted seedling
1. test material is a tea-tree tissue culture seedling, and the explant source is axillalry bud.Choose highly for about 5-6cm, the more consistent group training seedling of growing state, be transferred in the MS medium about 10 days strong sprouts, preceding 3 days of test bottle is opened, be put in the greenhouse hardening 3 days.
2. tissue cultivating seedling is taken out from blake bottle, clean the medium of bottom, and the brown callus that base portion produces is removed, use the carbendazim solution rinsing.
(2) processing of taking root of tea-tree tissue culture seedling
Adopt certain density HORMONE TREATMENT tea-tree tissue culture seedling base portion, then the matrix that its cuttage is gone into to be mixed (peat: perlite: vermiculite=2: 1: 1).
(3) take root the environment control and the management of tissue cultivating seedling
1. temperature control: adopt the cooling effect of sunshade net and switch, axial flow blower and the water Lian in skylight and the realization of hot blast warming-up device to temperature controlling, the growing environment temperature of tea-tree tissue culture seedling is controlled between 25 ℃～35 ℃.
2. humidity control: adopt spraying system that the air humidity in the tissue cultured seedling growth environment is adjusted, make it be controlled at 60%～90%.
3. intensity of illumination: transplant about initial stage (30) day its processings of sheltering from heat or light of carrying out 70% at tissue cultivating seedling, prevent that tissue cultivating seedling is because of the too strong death of illumination.Carry out full exposure after 30 days.
(4) final-period management of tissue cultivating seedling
Tissue cultivating seedling is after taking root, and it is consistent with cuttage seeding with environmental management to apply fertilizer.
Embodiment three:
With taking root of middle tea 102 tissue cultivating seedling is example, and directly the specific descriptions of rooting method are as follows:
(1) acquisition of tea-tree tissue culture transplanted seedling
1. test material is a tea-tree tissue culture seedling, and the explant source is axillalry bud.Choose highly for about 5-6cm, the more consistent group training seedling of growing state, be transferred in the MS medium about 10 days strong sprouts, preceding 3 days of test bottle is opened, be put in the greenhouse hardening 3 days.
2. tissue cultivating seedling is taken out from blake bottle, clean the medium of bottom, open and the brown callus that base portion produces is removed, use the carbendazim solution rinsing.
(2) processing of taking root of tea-tree tissue culture seedling
Adopt certain density HORMONE TREATMENT tea-tree tissue culture seedling base portion, then the matrix that its cuttage is gone into to be mixed (peat: perlite: vermiculite=2: 1: 1).
(3) take root the environment control and the management of tissue cultivating seedling
1. temperature control: adopt the cooling effect of sunshade net and switch, axial flow blower and the water Lian in skylight and the realization of hot blast warming-up device to temperature controlling, the growing environment temperature of tea-tree tissue culture seedling is controlled between 25 ℃～35 ℃.
2. humidity control: adopt spraying system that the air humidity in the tissue cultured seedling growth environment is adjusted, make it be controlled at 60%～90%.
3. intensity of illumination: transplant about initial stage (30) day its processings of sheltering from heat or light of carrying out 70% at tissue cultivating seedling, prevent that tissue cultivating seedling is because of the too strong death of illumination.Carry out full exposure after 30 days.
(4) final-period management of tissue cultivating seedling
Tissue cultivating seedling is after taking root, and it is consistent with cuttage seeding with environmental management to apply fertilizer.
Need not further to elaborate, believe and adopt the disclosed content in front, those skilled in the art can use the present invention to greatest extent.Therefore, the preferred specific embodiments of front is interpreted as only illustrating, but not limits the scope of the invention by any way.
Claims (4)
1. method for directly taking root of tea-tree tissue culture seedling is characterized in that realizing by following steps:
(1) hardening is handled before the tea-tree tissue culture seedling rooting: test material is a tea-tree tissue culture seedling, the explant source is axillalry bud, choose highly for about 5-6cm, the more consistent group training seedling of growing state, be transferred in the MS medium about 10 days strong sprouts, bottle was opened in preceding 3 days in test, be put in the greenhouse and practiced seedling 3 days;
(2) the tissue cultivating seedling processing of taking root: adopt different root-promoting hormone and variable concentrations, and adopt different dipping the processing time, after the tissue cultivating seedling base portion was handled, directly cuttage went in the matrix to take root;
(3) the take root investigation of situation: since the 20th day, the situation of taking root of tea-tree tissue culture seedling is carried out counting statistics, relatively the situation of the taking root difference between different disposal.
2. method for directly taking root of tea-tree tissue culture seedling according to claim 1 is characterized in that: the environmental temperature that suitable tissue cultivating seedling is taken root in the step (2) is controlled between 25 ℃～35 ℃.
3. method for directly taking root of tea-tree tissue culture seedling according to claim 1 is characterized in that: the ambient humidity that suitable tissue cultivating seedling is taken root in the step (2) is controlled at 60%～90%.
4. method for directly taking root of tea-tree tissue culture seedling according to claim 1 is characterized in that: it is peat that the matrix that tissue cultivating seedling is taken root in the step (2) adopts volume ratio: vermiculite: perlite=2: 1: 1.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569266B (en) * | 2009-06-08 | 2011-05-18 | 丽水市农业科学研究院 | Method for propagating tea plants |
| CN102726296A (en) * | 2012-07-04 | 2012-10-17 | 安徽农业大学 | Method for establishing tissue culture regeneration system of tea tree |
| CN102860229A (en) * | 2012-08-05 | 2013-01-09 | 肖勇 | Tea tree tray-soilless seedling substrate |
| CN102934603A (en) * | 2012-10-30 | 2013-02-20 | 安徽农业大学 | Asexual rapid propagation method of tea trees and root taking culture solution |
| CN107278686A (en) * | 2017-06-06 | 2017-10-24 | 山东大丰园农业有限公司 | A kind of American red-maple tissue culture outside sprout-cultivating-bottle radication agent and method for culturing seedlings |
| CN107926416A (en) * | 2017-10-19 | 2018-04-20 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of implantation methods of the wild stone precipice tea in Guangxi |
| CN115968786A (en) * | 2023-02-16 | 2023-04-18 | 云南省农业科学院茶叶研究所 | Culture medium and culture method for tea tree tissue culture |
-
2005
- 2005-09-28 CN CN 200510060923 patent/CN1742542A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569266B (en) * | 2009-06-08 | 2011-05-18 | 丽水市农业科学研究院 | Method for propagating tea plants |
| CN102726296A (en) * | 2012-07-04 | 2012-10-17 | 安徽农业大学 | Method for establishing tissue culture regeneration system of tea tree |
| CN102860229A (en) * | 2012-08-05 | 2013-01-09 | 肖勇 | Tea tree tray-soilless seedling substrate |
| CN102934603A (en) * | 2012-10-30 | 2013-02-20 | 安徽农业大学 | Asexual rapid propagation method of tea trees and root taking culture solution |
| CN102934603B (en) * | 2012-10-30 | 2014-07-09 | 安徽农业大学 | Asexual rapid propagation method of tea trees and root taking culture solution |
| CN107278686A (en) * | 2017-06-06 | 2017-10-24 | 山东大丰园农业有限公司 | A kind of American red-maple tissue culture outside sprout-cultivating-bottle radication agent and method for culturing seedlings |
| CN107926416A (en) * | 2017-10-19 | 2018-04-20 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of implantation methods of the wild stone precipice tea in Guangxi |
| CN115968786A (en) * | 2023-02-16 | 2023-04-18 | 云南省农业科学院茶叶研究所 | Culture medium and culture method for tea tree tissue culture |
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