CN106688589B - Cotton test tube seedling trench method for transplanting - Google Patents
Cotton test tube seedling trench method for transplanting Download PDFInfo
- Publication number
- CN106688589B CN106688589B CN201710028477.4A CN201710028477A CN106688589B CN 106688589 B CN106688589 B CN 106688589B CN 201710028477 A CN201710028477 A CN 201710028477A CN 106688589 B CN106688589 B CN 106688589B
- Authority
- CN
- China
- Prior art keywords
- trench
- grafting
- test tube
- culture
- cotton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Forests & Forestry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Wood Science & Technology (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses cotton test tube seedling trench method for transplanting.The present invention provides a kind of cotton test tube method for transplanting, includes the following steps: 1) to graft cotton test tube seedling, obtain grafting；2) grafting is cultivated in the trench for being covered with cool canopy, obtains grafting after trench culture；3) grafting after the trench culture is moved under the cool canopy of ground and is cultivated, obtain grafting after cool canopy culture；4) transplantation of seedlings crop field will be grafted after the cool canopy culture, realize the transplantation of seedlings of cotton test tube；The width of the trench is 50-100cm, depth 80-120cm.The experiment proves that trench is formed by subenvironment condition, is conducive to test tube seedling graft and transplantation and survives, with this transplantation technique, northern summer high temperature, Qiang Guang, be air-dried under conditions of still be able to carry out test tube transplantation of seedlings.
Description
Technical field
The present invention relates to field of biotechnology more particularly to a kind of cotton test tube seedling trench method for transplanting.
Background technique
The seedling that tissue cultures come out is referred to as test tube seedling or tissue-cultured seedling.Test tube seedling long term growth is in constant temperature, high humidity, dim light, nothing
It is huge with external environment difference in the closed environment of bacterium.It is influenced by this environment, cotton test tube seedling grows thin and delicate, cauline leaf table
Face cuticula is undeveloped；The photosynthesis of chloroplaset is poor；Stoma of Leaves number is few, poor activity；Root system is undeveloped, absorption function
Difference；It is poor to the adaptability and resistivity of adverse circumstance.
The ability that exactly internal and external environment difference is big, test tube seedling adapts to external environment is poor, in northern dry weather, directly transplants
Cotton test tube shoot survival percent is very low.The method for generalling use graft and transplantation test tube seedling (does scion with test tube seedling, grafting is sent out in root system
On the cotton seedling reached).But northern summer (7,8,9 three months) temperature is high, and illumination is extremely strong, it is air-dried, even if
Graft and transplantation is also that survival rate is very low.For cotton, tissue culture breaks up test tube seedling will be by embryonic differentiation approach, relative to it
His plant is more difficult, and cotton test tube seedling transplanting survival rate directly affects the success or failure of entire tissue culture work.
Summary of the invention
The object of the present invention is to provide a kind of cotton test tube method for transplanting.
Method provided by the invention, includes the following steps:
1) cotton test tube seedling is grafted, obtains grafting；
2) grafting is cultivated in the trench for being covered with cool canopy, obtains grafting after trench culture；
3) grafting after the trench culture is moved into progress cool canopy culture under the cool canopy of ground, is grafted after obtaining cool canopy culture
Seedling；
4) transplantation of seedlings crop field will be grafted after the cool canopy culture, realize the transplantation of seedlings of cotton test tube；
The width of the trench is 50-100cm, depth 80-120cm.
In the above method, the width of the trench is 80cm, depth 100cm.
In the above method, 25 DEG C -30 DEG C or so of temperature, humidity in the trench are 70%-90% or so, intensity of illumination
For 1500-2200LX or so.
Or the cool canopy is rain-proof shade.
In the above method, the time of the cotton test tube method for transplanting is summer.
In the above method, the place of the cotton test tube method for transplanting is north of China, and specially Shanxi, embodiment exists
Yuncheng, Shanxi carries out.
Yuncheng, Shanxi summer mean daily temperature is 30-40 DEG C or so, per day intensity of illumination be 3000-4000LX or so,
Per day air humidity is 30%-70% or so.
In the above method, in step 2), it includes following A and B that the grafting is cultivated in trench:
A, the grafting covering film culture in trench is placed in remove described thin to the grafting wound healing
Film obtains the grafting of removal film；
It is above-mentioned be placed in culture to the grafting wound healing in trench be specially after being placed in trench the 7th day beginnings discharge hole lead to
Gas, it is ascending, until interface obviously expands wound healing.
B, the grafting of the removal film is continued to place in trench and is cultivated, obtain grafting after trench transplanting.
In the above method, in step A, the incubation time is 20-30 days；
In step B, the incubation time is 3-5 days.
In the above method, in step 3), the cool canopy incubation time is 1-2 weeks；
Moved under sunlight daily during the mode of the cool canopy culture for several times, time first short and then long, generally from the morning 10 when
When to afternoon 4, remaining time is in cool canopy.
Another object of the present invention is to provide a kind of cotton grafting seedling trench method for transplanting.
Method provided by the invention includes the following steps: the step 2) -4 in the above method).
In the above method, the cotton test tube seedling is No. 35 test tube seedlings of middle cotton or other kind test tube seedlings；
The stock that uses grafted is sea island cotton seedling or the strong cotton variety of other well developed root systems, resistance of reverse.
Environmental condition directly affects the effect of test tube seedling graft and transplantation, it is important to light during controlling grafting, temperature, wet.
The experiment of the invention proves that trench is formed by subenvironment condition, the required dim light of test tube seedling grafting is met
(1500-2200LX), suitable constant temperature (25 DEG C -30 DEG C), suitable humidity (70%-90%) are conducive to test tube seedling grafting and move
Cultivation survives.With this transplantation technique, northern summer high temperature, Qiang Guang, be air-dried under conditions of still be able to carry out test tube seedling shifting
It plants.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1, cotton test tube seedling trench method for transplanting
1, the preparation of trench and graft
1) trench excavates
Yuncheng, Shanxi July summer digs one long under the shade of Cotton Research Institute, Shanxi Academy of Agricultural Sciences solarium:
It is 200cm or so, wide: the trench (under the cool canopy of common rain-proof) of 80cm, depth: 100cm, 25 DEG C of temperature or so in trench, ground
Humidity in ditch is 80% or so, and the intensity of illumination in trench is 1500LX or so.
2), graft
The sea island cotton seedling with 3-4 piece true leaf in flowerpot is chosen as stock, with No. 35 tissue cultures of cotton in 50 plants
Regenerating cotton test tube seedling, (hypocotyl of 35 cotton aseptic seedlings is that explant carries out tissue cultures in, passes through embryonic differentiation approach
The cotton regenerated plant obtained) scion is done respectively is grafted, obtain the cotton test tube seedling for grafting in flowerpot, as grafting.
2, trench is transplanted
It will be placed in the trench that 1 obtains after the cotton test tube seedling covering thin transparent film for grafting in flowerpot that above-mentioned 1 obtains,
Carry out following trench transplanting culture:
The first beginning discharge hole ventilation in the 7th day after being placed in trench, it is ascending, until interface obviously expands and (illustrates notch
Through healing, survive substantially), it removes interface cotton rope and removes transparent membrane completely, obtain within about 20 days grafting in for removal film
The cotton test tube seedling of flowerpot；
Continue the cotton test tube seedling for grafting in flowerpot for removing film to place culture 3 days in trench again, obtains trench shifting
Grafting after cultivation.
3, cool canopy culture
The test tube seedling of grafting survival in trench is moved on under the cool canopy on ground and cultivates one week (period moves under sunlight number daily
It is secondary, time first short and then long, generally from the morning 10 when afternoon 4), obtain grafting after cool canopy culture.
4, crop field is moved into
Grafting after cool canopy culture is moved into crop field.
50 plants of test tube seedling of test grafting, survives 35 plants, survival rate 70%.
Embodiment 2, cotton test tube seedling trench method for transplanting
Essentially identical with the method for embodiment 1, different is only that No. 35 tissue cultures of cotton in 50 plants are regenerated cotton test tube
Seedling replaces with No. 3 tissue cultures regeneration cotton test tube seedlings of 30 plants of nasal mucus cottons.
As a result, 30 plants of test tube seedling of test grafting, survives 18 plants, survival rate 60%.
Claims (7)
1. a kind of cotton test tube method for transplanting, includes the following steps:
1) cotton test tube seedling is grafted, obtains grafting；
2) grafting is cultivated in the trench for being covered with cool canopy, obtains grafting after trench culture；
3) grafting after the trench culture is moved into progress cool canopy culture under the cool canopy of ground, obtains grafting after cool canopy culture；
4) transplantation of seedlings crop field will be grafted after the cool canopy culture, realize the transplantation of seedlings of cotton test tube；
The width of the trench is 50-100cm, depth 80-120cm；
25 DEG C -30 DEG C of temperature in the trench, humidity 70%-90%, intensity of illumination 1500-2200LX；
The time of the cotton test tube method for transplanting is summer；
In step 2, it includes following A and B that the grafting is cultivated in trench:
A, the grafting covering film culture in trench is placed in remove the film to the grafting wound healing, obtain
To the grafting of removal film；
B, the grafting of the removal film is continued to place in trench and is cultivated, obtain grafting after trench culture.
2. according to the method described in claim 1, it is characterized by: the width of the trench is 80cm, depth 100cm.
3. method according to claim 1 or 2, it is characterised in that: cool canopy is rain-proof shade.
4. method according to claim 1 or 2, it is characterised in that: during the place of the cotton test tube method for transplanting is
The state north.
5. method according to claim 1 or 2, it is characterised in that:
The incubation time of step A is 20-30 days；
The incubation time of step B is 3-5 days.
6. method according to claim 1 or 2, it is characterised in that:
In step 3), the cool canopy incubation time is 1-2 weeks.
7. according to the method described in claim 1, it is characterized by: the stock that uses grafted is sea island cotton seedling or other
The strong cotton variety of well developed root system, resistance of reverse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710028477.4A CN106688589B (en) | 2017-01-16 | 2017-01-16 | Cotton test tube seedling trench method for transplanting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710028477.4A CN106688589B (en) | 2017-01-16 | 2017-01-16 | Cotton test tube seedling trench method for transplanting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106688589A CN106688589A (en) | 2017-05-24 |
| CN106688589B true CN106688589B (en) | 2019-11-15 |
Family
ID=58908422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710028477.4A Expired - Fee Related CN106688589B (en) | 2017-01-16 | 2017-01-16 | Cotton test tube seedling trench method for transplanting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106688589B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950340A (en) * | 2017-11-01 | 2018-04-24 | 河北省农林科学院遗传生理研究所 | A kind of cotton test tube method for transplanting of high-survival rate |
| CN109804881A (en) * | 2018-11-22 | 2019-05-28 | 安徽省农业科学院棉花研究所 | A kind of cotton field-transplanting wintering method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1685795A (en) * | 2005-04-30 | 2005-10-26 | 山西省农业科学院棉花研究所 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
| CN1985573A (en) * | 2006-12-30 | 2007-06-27 | 中国农业科学院棉花研究所 | Non-embryo transgenic process for preparing transgenic cotton |
| CN103782811B (en) * | 2014-02-26 | 2015-09-02 | 武汉市农业科学研究所 | A kind of watermelon plantlet in vitro test tube micro-grafting method |
| CN105103972A (en) * | 2015-09-25 | 2015-12-02 | 山西省农业科学院棉花研究所 | Temperature-controlled grafting method for regenerated plants of transgenic cotton |
-
2017
- 2017-01-16 CN CN201710028477.4A patent/CN106688589B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1685795A (en) * | 2005-04-30 | 2005-10-26 | 山西省农业科学院棉花研究所 | Substrate and method of cotton regeneration test tube seedling direct transplantation |
| CN1985573A (en) * | 2006-12-30 | 2007-06-27 | 中国农业科学院棉花研究所 | Non-embryo transgenic process for preparing transgenic cotton |
| CN103782811B (en) * | 2014-02-26 | 2015-09-02 | 武汉市农业科学研究所 | A kind of watermelon plantlet in vitro test tube micro-grafting method |
| CN105103972A (en) * | 2015-09-25 | 2015-12-02 | 山西省农业科学院棉花研究所 | Temperature-controlled grafting method for regenerated plants of transgenic cotton |
Non-Patent Citations (1)
| Title |
|---|
| 陆地棉体细胞胚胎发生和植株再生体系的建立;林君;《中国优秀博硕士学位论文全文数据库（硕士） 农业科技辑》;20060915(第09期);D047-80页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106688589A (en) | 2017-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102119660B (en) | Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse | |
| CN100559934C (en) | A kind of primulina tabacum (Primulina tabacum Hance) tissue culture propagating and field planting method | |
| CN105075863B (en) | A kind of Paeonia papaveracea rapid propagation method | |
| CN103190344B (en) | Tissue culture method of fargesii | |
| CN103688855B (en) | A kind of leaflet red bean isolated seed embryo and plant regeneration method | |
| CN110771503B (en) | Sugar-free cultivation strong seedling transplanting method for detoxified sweet potato seedlings | |
| CN109845500B (en) | Method for raising seedlings of populus tomentosa by cutting tender branches | |
| CN104686362A (en) | Butterfly orchid tissue culturing method | |
| CN104871945A (en) | Heuchera micrantha tissue culture propagation ex-vitro rooting seedling hardening method | |
| CN104996169A (en) | Method for grafting China rose and rose on multiflora rose | |
| CN106688589B (en) | Cotton test tube seedling trench method for transplanting | |
| CN103371103B (en) | Rapid propagation method for tissue culture of Rhododendron delavayi Franch | |
| CN108029559B (en) | Method for quickly cultivating ilex latifolia tissue culture seedlings | |
| CN1685795A (en) | Substrate and method of cotton regeneration test tube seedling direct transplantation | |
| CN104871967A (en) | Method for hardening and transplanting tissue cultured opisthopapus taihangensis seedlings | |
| CN107232061B (en) | A kind of special culture media and tissue culture method of rapid induction bletilla bulb | |
| CN104904603A (en) | Spring begonia tissue culture breeding method | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN103348915B (en) | Method for strong seedling and rooting culture of strawberries | |
| WO2022171212A2 (en) | Method for ex vivo culturing of thornless green prickly ash zanthoxylum armatum dc | |
| CN1768576A (en) | Peony standardized quick-breeding method | |
| Adelberg et al. | Photoautotrophic shoot and root development for triploid melon | |
| CN110679457B (en) | Method for improving transplanting survival rate of tissue culture seedlings of Nicotiana benthamiana | |
| CN104521527B (en) | Rapid propagation method for hyacinths | |
| CN103155866A (en) | Malus zumi tissue culture rapid propagation seedling raising method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191115 Termination date: 20220116 |