CN105103972A - Temperature-controlled grafting method for regenerated plants of transgenic cotton - Google Patents

Abstract

The invention discloses a temperature-controlled grafting method for regenerated plants of transgenic cotton. The method comprises the following specific operation steps: 1, preparation of stock seedlings, wherein gossypium barbadense seeds which are good in disease resistance and long in growing period are selected and sowed in a small basin, the height of the small basin is 10-12 cm, the diameter of an upper opening is 10-12 cm, the diameter of a lower opening is 9 cm, and when 1-4 main leaves emerge, the seedlings are reserved for standby application; 2, grafting and temperature control. By means of the method, the phenomenon that the total plants die due to the fact that at the high temperature time in summer, stock roots or the upper portions of the regenerated plants of the transgenic cotton are prone to being rotten can be effectively reduced, when the atmosphere temperature is equal to or greater than 35 DEG C in summer, after the grafted seedlings are grafted and 1-7 days later, and when the wrapped space temperature is less than or equal to 28 DEG C, the survival rate of the grafted seedlings can reach over 95%, and the survival rate of the regenerated plants of the transgenic cotton can be raised by over 15% in a high temperature season.

Description

A kind of transgenic cotton regenerated plant temperature control grafting

Technical field

The present invention relates to biological technical field, be specifically related to a kind of transgenic cotton regenerated plant temperature control grafting.

Background technology

Present stage, the grafting of transgenic cotton regenerated plant routine is grafting, first transgenic cotton regenerated plant test tube cap is opened and carry out indoor in-bottle seeding 1-2 days, on (cotton) stock of transgenic cotton regenerated plant grafting being planted in basin alms bowl in greenhouse or solarium again, whole cotton seedling 7 ~ 8 days are sealed together with basin alms bowl again with plastic film, then progressively leak informaton, finally take down plastic film.But summer high temperature period stock root or transgenic cotton regenerated plant grafting top perishable and cause whole strain dead render transgenic cotton survival rate not high.In order to solve above-mentioned existing problem, (patent announcement number is respectively CN201595049U to have investigated a series of transgene cotton grafting method at present, CN202340415U, CN102893813A), although improve the survival rate of grafting to a certain extent, also do not reach anticipation object.

Summary of the invention

The object of the invention is to provide a kind of transgenic cotton regenerated plant temperature control grafting, and it can solve problem existing in background technology effectively.

In order to solve problem existing in background technology, it specifically comprises following operating process:

The preparation of step one, rootstock seedling: select disease-resistant good, sea-island cotton planting seed (10 ~ 12cm is high, back cut diameter 10 ~ 12cm, lower port diameter 9cm) in little basin that vegetative period is longer, emerge to stand-by during 1 ~ 4 true leaf;

Step 2, grafting and temperature control: the grafting of (1) transgenosis regeneration strain, on the stock of basin alms bowl, ties up with rope; (2) intersect fixing around the grafting of basin alms bowl with iron wire, grafting peripheral with plastic film parcel to basin upper edge edge; (3) plastic foil wrapped up is tightened, and head inserts in plastic foil; (4) place of space temperature≤28 DEG C of controling environment put into by grafting basin alms bowl; Plastic foil bottom osculum is first decontroled when (5) the 7th days; (6) 8th ~ 10 day every day progressively plastic foil; Thoroughly take down plastic foil when (7) the 11st days, untie the tied rope of grafting and stock; (8) grafting band basin was directly put into solarium and move into large basin seedling to gathering in the crops seed when transgenosis regeneration strain is transplanted and slightly grown up to large.

Owing to have employed above technical scheme, the present invention has following beneficial effect: effectively can reduce summer high temperature period stock root or transgenic cotton regenerated plant grafting top perishable and cause the phenomenon of whole strain death, when atmospheric air temperature >=35 DEG C in summer, adopt 1 ~ 7 day after controlling grafting grafting, parcel space temperature≤28 DEG C, grafting survival rate can reach more than 95%, in the high temperature time, transgenic cotton regenerated plant shoot survival percent can be improved more than 15%.

Embodiment

The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below the technical scheme in the embodiment of the present invention being clearly and completely described.

Embodiment 1

A kind of transgenic cotton regenerated plant temperature control grafting, it specifically comprises following operating process:

The preparation of step one, rootstock seedling: select disease-resistant good, sea-island cotton planting seed (10 ~ 12cm is high, back cut diameter 10 ~ 12cm, lower port diameter 9cm) in little basin that vegetative period is longer, emerge to stand-by during 1 ~ 4 true leaf;

Step 2, grafting and temperature control: the grafting of (1) transgenosis regeneration strain, on the stock of basin alms bowl, ties up with rope; (2) intersect fixing around the grafting of basin alms bowl with iron wire, grafting peripheral with plastic film parcel to basin upper edge edge; (3) plastic foil wrapped up is tightened, and head inserts in plastic foil; (4) place of space temperature≤28 DEG C of controling environment put into by grafting basin alms bowl; Plastic foil bottom osculum is first decontroled when (5) the 7th days; (6) 8th ~ 10 day every day progressively plastic foil; Thoroughly take down plastic foil when (7) the 11st days, untie the tied rope of grafting and stock; (8) grafting band basin was directly put into solarium and move into large basin seedling to gathering in the crops seed when transgenosis regeneration strain is transplanted and slightly grown up to large.

Embodiment 2

A kind of transgenic cotton regenerated plant temperature control grafting, in the summer high temperature period (atmospheric temperature >=35 DEG C), the grafting of cotton transgenic regeneration strain grown in little basin on disease-resistant good, the sea-island cotton stock that vegetative period is longer of 1 ~ 4 true leaf, with the place after plastic film wrapped grafting basin alms bowl, grafting basin alms bowl being put into space temperature≤28 DEG C of controling environment, transgenosis regeneration strain space temperature decreases, stock root imputrescibility and cause whole plant dead, grafting part is also rotted and dead.Therefore, transgenosis regeneration strain transplanting success is very high, so this is a kind of simple, ideal transgenosis regenerated plant transplantation method.

Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art should be appreciated that it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims (1)

1. a transgenic cotton regenerated plant temperature control grafting, is characterized in that it specifically comprises following operating process:

The preparation of step one, rootstock seedling: select disease-resistant good, sea-island cotton planting seed (10 ~ 12cm is high, back cut diameter 10 ~ 12cm, lower port diameter 9cm) in little basin that vegetative period is longer, emerge to stand-by during 1 ~ 4 true leaf;

Step 2, grafting and temperature control: the grafting of (1) transgenosis regeneration strain, on the stock of basin alms bowl, ties up with rope; (2) intersect fixing around the grafting of basin alms bowl with iron wire, grafting peripheral with plastic film parcel to basin upper edge edge; (3) plastic foil wrapped up is tightened, and head inserts in plastic foil; (4) place of space temperature≤28 DEG C of controling environment put into by grafting basin alms bowl; Plastic foil bottom osculum is first decontroled when (5) the 7th days; (6) 8th ~ 10 day every day progressively plastic foil; Thoroughly take down plastic foil when (7) the 11st days, untie the tied rope of grafting and stock; (8) grafting band basin was directly put into solarium and move into large basin seedling to gathering in the crops seed when transgenosis regeneration strain is transplanted and slightly grown up to large.