CN102334446A - Method for breeding perennial triticale-thinopyrum elongatum hybrid - Google Patents
Method for breeding perennial triticale-thinopyrum elongatum hybrid Download PDFInfo
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- CN102334446A CN102334446A CN2010102342965A CN201010234296A CN102334446A CN 102334446 A CN102334446 A CN 102334446A CN 2010102342965 A CN2010102342965 A CN 2010102342965A CN 201010234296 A CN201010234296 A CN 201010234296A CN 102334446 A CN102334446 A CN 102334446A
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Abstract
The invention discloses a method for breeding a perennial triticale-thinopyrum elongatum hybrid. The method comprises the following steps that 1, triticale I is bred out through wheat as a bridge material and the triticale I is hybridized with thinopyrum elongatum; 2, the thinopyrum elongatum subjected to hybridization is transplanted to a greenhouse in October; light is added at night in middle and late February; and when the thinopyrum elongatum flowers and flies pollen on April 25, orchid triticale I is obtained; 3, a triticale plant of the orchid triticale I is transplanted to a flowerpot and is subjected to heading; and when gynoecium stigmas develop fully, the triticale plant is transferred in thinopyrum elongatum and carries out open pollination to produce F0; 4, ears of the F0 are harvested according to combinations, dried in air, threshed and stored in early June; 5, triticale is hybridized with thinopyrum elongatum in early September to produce F0 seeds; the F0 seeds are subjected to accelerating germination to produce seedlings; and the seedlings are transplanted to screen houses in farmland in late September and are bred into F1 seedlings; 6, the F1 seedlings are transferred to a room and are subjected to chromosome reduplication; and 7, the F1 seedlings subjected to chromosome reduplication are transplanted to a greenhouse and are subjected to strengthened management, wherein the survived F1 seedlings are the perennial triticale-thinopyrum elongatum hybrids. The method can breed out a novel species of the perennial triticale-thinopyrum elongatum hybrid easy for hybridizing with common wheat.
Description
Technical field
The present invention relates to the crop breeding method, particularly relate to the breeding method of a kind of perennial little black wheat of laying down.
Background technology
At present, cultivate high yield, high-quality, many anti-wide suitable breakthrough new variety of wheat, be unable to do without the innovation of germ plasm resource.Therefore, many in the world well-known scientists have proposed the breeding theory of " germplasm is supreme ", and China only pays attention to breeding of new variety for a long time, and have ignored the innovation of germ plasm resource, therefore cause germ plasm resource deficient, and breeding does not have the situation of new breakthrough.Show according to research, germ plasm resource is provided in order to give the super high-yielding breeding, with the excellent genes importing common wheat of external source kind, synthetic new species and type, utilizing these germplasms to hybridize is to create the more feasible method of cultivating new varieties again.
Summary of the invention
The breeding method that the purpose of this invention is to provide a kind of perennial little black wheat of laying down can be cultivated an easy perennial little black wheat new germ plasm of laying down of hybridizing with common wheat.
For realizing above-mentioned purpose, technical scheme of the present invention is:
The breeding method of a kind of perennial little black wheat of laying down may further comprise the steps:
(1) being the bridge material with the wheat, cultivating " triticale No. 1 ", serves as maternal and long fringe couchgrass hybridization with " triticale No. 1 ";
(2) October long fringe couchgrass is transplanted to the greenhouse, added light night for during to the middle ten days and the last ten days in February long fringe couchgrass,, obtain " blue triticale No. 1 " to duration fringe on the 25th couchgrass loose powder of blooming in April;
(3) with the plantlet of transplant of triticale in " blue triticale No. 1 " in flowerpot; Put in order fringe during heading and castrate, treat that pistil stigma is reached maturity after, move in the long fringe couchgrass clump; Let its open pollination; Couchgrass was rocked in manual work when bloomed long fringe couchgrass every day, let the pollen of long fringe couchgrass be scattered on the column cap of triticale, obtained F
0
At the beginning of (4) 6 months with solid F
0Tassel is regained by combination and is dried, threshing, collection;
(5) triticale hybridizes the F that ties with long fringe couchgrass early September
0Seed obtains seedling by combination vernalization, and late September in the solarium, field, is cultivated strong sprout with seedling replanting, obtains the F of triticale and couchgrass hybridization
1Seedling;
(6) late November is with F
1Seedling is fetched the indoor chromosome doubling of carrying out by combination;
(7) double the back transplanting and to the greenhouse, strengthen management, recover growth, double into plant alive and be the perennial little black wheat of laying down.
Preferably, adding the light time in the step according to the invention (2) adopts per 7～10m in the greenhouse
2The mercury lamp of adorning a 450w shines.
Preferably, F during vernalization in the step according to the invention (5)
0Seed is placed in the sand table in the illumination box by combination.
Preferably, adopt colchicin doubling monoploids method in the step according to the invention (6) during chromosome doubling.
Therefore compared with prior art, the present invention has following beneficial effect:
(1) because long fringe couchgrass is long breeding time; Bloom general 7～August; Make male parent and will guarantee that flower synchronization must regulate the florescence, step of the present invention (2) just adopts the method that adds light night to regulate the florescence, makes that long fringe couchgrass is blooming by the end of April; Add light night and heat again and can guarantee 24 hours illumination, regulating effect is better;
(2) couchgrass is rocked in the every day of manual work when long fringe couchgrass blooms in the step of the present invention (3); Let couchgrass pollen be scattered on the column cap of triticale; So maternal triticale all can be accepted the male parent couchgrass any time in 24 hours pollen insemination has significantly improved ripening rate;
(3) " blue triticale No. 1 " is hexaploid, and 42 chromosomes are arranged, and wherein 28 is the chromosome of wheat; Article 14, be the chromosome of rye, solid normal, and long fringe couchgrass is a decaploid; 70 chromosomes are arranged, and both hybridize the back first generation owing to be different inter-genera distant hybridizations, and chromosome just occurs unpaired; The first generation is shaky, so step of the present invention (6) adopts colchicin doubling monoploids method to double;
(4) the perennial little black wheat of laying down cultivated of the present invention generally doubles survival rate all 40---and about 70%; The AB chromosome set of the existing wheat of this newtype has the R chromosome set of rye again; The E chromosome set that also has long fringe couchgrass; Perennial little black 150 centimetres of the wheat plant heights of newly breeding of laying down, plant is compact, and tillering ability is strong, the percentage of earbearing tiller is high, blade is thick,, the narrow length of leaf look dark green uprightly goes up and lifts, fringe is rectangular greatly, about 40 centimetres of spike lengths, about 35 of spikelet numbers; Indivedual plant have branch, spend more many realities, high anti-bar, leaf, black stem rust and powdery mildew; Combining the merit that wheat, rye, couchgrass three belongs to, is unusual precious wheat breeding germ plasm resource, we can say that therefore the present invention is that the excellent genes of rye, couchgrass is incorporated into effective way is provided in the common wheat; Also broken the isolation between the species, solved wheat, rye, couchgrass intergeneric cross and be difficult for solid problem.
Embodiment
To combine accompanying drawing and specific embodiment to specify the present invention below, be used for explaining the present invention in this illustrative examples of the present invention and explanation, but not as to qualification of the present invention.
Cultivate the perennial little black wheat of laying down according to following steps:
(1) being the bridge material with the wheat, cultivating " triticale No. 1 ", serves as maternal and long fringe couchgrass hybridization with " triticale No. 1 ";
(2) October long fringe couchgrass is transplanted to the greenhouse, added light night for during to the middle ten days and the last ten days in February long fringe couchgrass, add per 7～10m in the light time employing greenhouse
2The mercury lamp of adorning a 450w shines, and to duration fringe on the 25th couchgrass loose powder of blooming in April, obtains " blue triticale No. 1 ";
(3) with the plantlet of transplant of triticale in " blue triticale No. 1 " in flowerpot; Put in order fringe during heading and castrate, treat that pistil stigma is reached maturity after, move in the long fringe couchgrass clump; Let its open pollination; Couchgrass was rocked in manual work when bloomed long fringe couchgrass every day, let the pollen of long fringe couchgrass be scattered on the column cap of triticale, obtained F
0
At the beginning of (4) 6 months with solid F
0Tassel is regained by combination and is dried, threshing, collection;
(5) triticale hybridizes the F that ties with long fringe couchgrass early September
0Seed obtains seedling, F during vernalization by combination vernalization
0Seed is placed in the sand table in the illumination box by combination, and late September in the solarium, field, is cultivated strong sprout with seedling replanting, obtains the F of triticale and couchgrass hybridization
1Seedling;
(6) late November is with F
1Seedling is fetched the indoor chromosome doubling of carrying out by combination, adopts colchicin doubling monoploids method when doubling;
(7) double the back transplanting and to the greenhouse, strengthen management, recover growth, double into plant alive and be the perennial little black wheat of laying down.
During practical operation, add the light time in the above-mentioned steps (2) to adopt per 7～10m in the greenhouse
2The mercury lamp of adorning a 450w shines; F during vernalization in the above-mentioned steps (5)
0Seed is placed on the above-mentioned colchicin doubling monoploids method that adopts when stating in the step (6) chromosome doubling in the sand table in the illumination box by combination.
More than the technical scheme that the embodiment of the invention provided is described in detail; Used specific case herein the principle and the embodiment of the embodiment of the invention are set forth, the explanation of above embodiment only is applicable to the principle that helps to understand the embodiment of the invention; Simultaneously, for one of ordinary skill in the art, according to the embodiment of the invention, the part that on the specific embodiment and range of application, all can change, in sum, this description should not be construed as limitation of the present invention.
Claims (4)
1. the breeding method of the perennial little black wheat of laying down is characterized in that may further comprise the steps:
(1) being the bridge material with the wheat, cultivating " triticale No. 1 ", serves as maternal and long fringe couchgrass hybridization with " triticale No. 1 ";
(2) October long fringe couchgrass is transplanted to the greenhouse, added light night for during to the middle ten days and the last ten days in February long fringe couchgrass,, obtain " blue triticale No. 1 " to duration fringe on the 25th couchgrass loose powder of blooming in April;
(3) with the plantlet of transplant of triticale in " blue triticale No. 1 " in flowerpot; Put in order fringe during heading and castrate, treat that pistil stigma is reached maturity after, move in the long fringe couchgrass clump; Let its open pollination; Couchgrass was rocked in manual work when bloomed long fringe couchgrass every day, let the pollen of long fringe couchgrass be scattered on the column cap of triticale, obtained F
0
At the beginning of (4) 6 months with solid F
0Tassel is regained by combination and is dried, threshing, collection;
(5) triticale hybridizes the F that ties with long fringe couchgrass early September
0Seed obtains seedling by combination vernalization, and late September in the solarium, field, is cultivated strong sprout with seedling replanting, obtains the F of triticale and couchgrass hybridization
1Seedling;
(6) late November is with F
1Seedling is fetched the indoor chromosome doubling of carrying out by combination;
(7) double the back transplanting and to the greenhouse, strengthen management, recover growth, double into plant alive and be the perennial little black wheat of laying down.
2. the breeding method of the perennial little black wheat of laying down according to claim 1 is characterized in that: adding the light time in the said step (2) adopts per 7～10m in the greenhouse
2The mercury lamp of adorning a 450w shines.
3. the breeding method of the perennial little black wheat of laying down according to claim 1 is characterized in that: F during vernalization in the said step (5)
0Seed is placed in the sand table in the illumination box by combination.
4. the breeding method of the perennial little black wheat of laying down according to claim 1 is characterized in that: adopt colchicin doubling monoploids method in the said step (6) during chromosome doubling.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102342965A CN102334446A (en) | 2010-07-22 | 2010-07-22 | Method for breeding perennial triticale-thinopyrum elongatum hybrid |
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| CN2010102342965A CN102334446A (en) | 2010-07-22 | 2010-07-22 | Method for breeding perennial triticale-thinopyrum elongatum hybrid |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250632A (en) * | 2013-05-27 | 2013-08-21 | 湖北省烟草科研所 | Propagation method for tobacco seeds by artificial supplementary pollination |
| RU2511752C2 (en) * | 2012-07-31 | 2014-04-10 | Владимир Николаевич Баринов | Method of growing herbs for green fodder and seeds on sod-podzol sandy loam soil of central non-black earth area |
| CN111202000A (en) * | 2020-01-20 | 2020-05-29 | 克劳沃(北京)生态科技有限公司 | Breeding method of triticale |
-
2010
- 2010-07-22 CN CN2010102342965A patent/CN102334446A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2511752C2 (en) * | 2012-07-31 | 2014-04-10 | Владимир Николаевич Баринов | Method of growing herbs for green fodder and seeds on sod-podzol sandy loam soil of central non-black earth area |
| CN103250632A (en) * | 2013-05-27 | 2013-08-21 | 湖北省烟草科研所 | Propagation method for tobacco seeds by artificial supplementary pollination |
| CN103250632B (en) * | 2013-05-27 | 2014-10-22 | 湖北省烟草科研所 | Propagation method for tobacco seeds by artificial supplementary pollination |
| CN111202000A (en) * | 2020-01-20 | 2020-05-29 | 克劳沃(北京)生态科技有限公司 | Breeding method of triticale |
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Application publication date: 20120201 |