CN102334414B - Culture method for perennial trititrigia - Google Patents
Culture method for perennial trititrigia Download PDFInfo
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- CN102334414B CN102334414B CN 201010236197 CN201010236197A CN102334414B CN 102334414 B CN102334414 B CN 102334414B CN 201010236197 CN201010236197 CN 201010236197 CN 201010236197 A CN201010236197 A CN 201010236197A CN 102334414 B CN102334414 B CN 102334414B
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- perennial
- wheat
- seedlings
- short time
- laying down
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- 238000012136 culture method Methods 0.000 title abstract 3
- 241000209140 Triticum Species 0.000 claims abstract description 25
- 235000021307 Triticum Nutrition 0.000 claims abstract description 25
- 210000000349 chromosome Anatomy 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims abstract description 10
- 239000004576 sand Substances 0.000 claims abstract description 4
- 238000009331 sowing Methods 0.000 claims abstract description 4
- 241000508725 Elymus repens Species 0.000 claims description 25
- 238000009395 breeding Methods 0.000 claims description 12
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 claims description 8
- 244000098338 Triticum aestivum Species 0.000 claims description 8
- 230000010152 pollination Effects 0.000 claims description 7
- 230000008774 maternal effect Effects 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 238000009396 hybridization Methods 0.000 claims description 5
- 229960001338 colchicine Drugs 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 241000894007 species Species 0.000 abstract description 3
- 241000700189 Hystrix <Rodentia> Species 0.000 abstract description 2
- 241000209125 Thinopyrum elongatum Species 0.000 abstract 3
- 241000238631 Hexapoda Species 0.000 abstract 1
- 210000005069 ears Anatomy 0.000 abstract 1
- 230000005070 ripening Effects 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000380130 Ehrharta erecta Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a culture method for a perennial trititrigia, which includes the following steps: (1) in overwintering period, a greenhouse is built in an Elytrigia elongata-sowing region, filament lamps start to be used for lighting in late February, and Elytrigia elongata ear and bloom in late April; (2) 'Lankao Aizao 8' plants are entirely castrated, transplanted into flowerpots, put into the Elytrigia elongata greenhouse and freely pollinated, and thereby hybrid first-generation seedlings F0 are obtained; (3) in early June, the ears of the F0 are dried in the sun, and are threshed, stored and prevented from being eaten by insects according to hybridized combination; (4) in early September, F0 seeds are sowed in sand trays and put into an illuminating incubator to grow seedlings; (5) in late September or early October, the F0 seeds are transplanted into a net house, tiller and develop, and thereby F1 seedlings are obtained; (6) in late November, chromosome doubling is carried out on the F1 seedlings indoors, so that the F1 seedlings can become amphidiploids, the F1 seedlings are transplanted into the field after doubling, and thereby the perennial trititrigia is obtained. The culture method synthesizes a new perennial trititrigia species hybridized with wheat and Elytrigia elongata.
Description
Technical field
The present invention relates to the crop breeding method, particularly relate to the breeding method of a kind of perennial wheat of laying down for a short time.
Background technology
At present, along with China's rapid economy development, the soil reduces, increased population, and shortage of water resources, the variation of ecological environmental condition and biological variation all bring to the agricultural production development to seriously influence.Ensure China's grain security effective supply, only rely on scientific and technological advances, cultivate better variety of crops, significantly improve the yield per unit area of crops.Cultivate new crop varieties, good germ plasm resource will be arranged.Wheat is China's staple food crop, and it is food with the wheat that there is the urban population more than 40% in the whole nation.The wheat breed of China output does not for a long time have breakthrough, and quality is unexcellent, and disease resistance, resistance are not strong, the shortcoming that adaptability is wideless, and tracing it to its cause is that wheat breed is not broken through.Can not educate good kind, key is the short and deficient of germ plasm resource.Long fringe couchgrass is a kind of perennial grass, couchgrass belongs to (Elytrigia), about 150 centimetres of plant heights, blade is thick narrow, about 50 centimetres of spike lengths, the elder reaches more than 80 centimetre, and general more than 20 of sign indicating numbers rare (small ear) are spent more, well developed root system, cold resistance is strong, high anti-wheat bar, leaf, black stem rust, and chromosome is 70 (10 times of bodies), if can transfer to its big fringe, leaflet, excellent genes such as disease-resistant in the common wheat, just the new varieties that can synthesize a grow wheat, however that Triticum and couchgrass belong to both affiliations is far away, is difficult for cross-fertile.
Summary of the invention
The breeding method that the purpose of this invention is to provide a kind of perennial wheat of laying down has for a short time synthesized the perennial wheat new species of laying down for a short time that a grow wheat-long fringe couchgrass hybridization forms.
For achieving the above object, technical scheme of the present invention is:
The breeding method of a kind of perennial wheat of laying down for a short time may further comprise the steps:
(1) phase of surviving the winter is built warm canopy with long fringe couchgrass sowing division, and late Febuary begins to add light with incandescent lamp, and long fringe couchgrass heading is bloomed at the bottom of April;
(2) with after the whole fringe castration of the plant of " short morning eight of Lankao ", transplant to flowerpot, put into long fringe couchgrass temperature canopy, allow its open pollination, every morning 4～6 o'clock and at 6 o'clock in afternoon aim at maternal plant and manually rock long fringe couchgrass, allow its pollen freely be scattered on the maternal column cap, obtain the F of common wheat and the hybridization of long fringe couchgrass
0
At the beginning of (3) 6 months with F
0Tassel dry, by the hybrid combination threshing, preserve, prevent worm food;
At the beginning of (4) 9 months with F
0Planting seed is placed in the illumination box and grows seedlings in sand table;
(5) at the beginning of 10 months by the end of September with F
0Be transplanted in the solarium, tiller, in strong sprout, obtain F
1Seedling;
(6) late November is indoor to F
1Seedling carries out chromosome doubling handles, and makes it become amphidiploid, doubles the back and transplants to the field, strengthens management, and obtains the perennial wheat of laying down for a short time.
Preferably, adding light time incandescent lamp irradiation area in the step of the present invention (1) is 7～9m
2
Preferably, the time of open pollination is more than ten day in the step of the present invention (2).
Preferably, line-spacing is 50～60 centimetres when transplanting in the step of the present invention (5), 30～50 centimetres of spacing in the rows.
Adopt colchicine chromosome doubling method when preferably, chromosome doubling is handled in the step of the present invention (6).
Therefore compared with prior art, the present invention has following beneficial effect:
(1) because " short morning eight of Lankao " is common wheat, belong to Triticum, to belong to both affiliations far away with long fringe couchgrass, be difficult for cross-fertile, and long fringe couchgrass is long breeding time, and begin to bloom general 7～August under the natural conditions, so will allow two kinds hybridize, the florescence is regulated earlier in step of the present invention (1) and (2), also filters out the common wheat kind of easy and long fringe couchgrass cross-fertile;
(2) the middle open pollination of step of the present invention (2) is more than ten day, can significantly improve ripening rate, in order to improve ripening rate, can also many do female parent with several common wheat kinds during practical operation, selects the high kind of ripening rate to hybridize therein;
(3) because " short morning eight of Lankao " be 6 times of bodies, 42 chromosomes are arranged, and long fringe couchgrass is 10 times of bodies, 70 chromosomes are arranged, become 38 times of bodies after the two hybridization, 56 chromosomes are arranged, so the monoploid hybrid first generation (F
1) shaky, in order to solve shaky problem, step of the present invention (6) adopts the colchicine method for doubling, allows it become amphidiploid, i.e. wheat-16 times of bodies of long fringe couchgrass;
(4) the general survival rate of the present invention is 40～60%, and the plant that survives after doubling namely becomes a new species type---the perennial wheat of laying down for a short time, 100～150 centimetres of the perennial wheat of breeding 1 of laying down for a short time---No. 23 general plant heights, about 50 centimetres of spike lengths, several about 25 of Xiao Hua, blade is little and go up to lift, and tillering capacity is extremely strong, percentage of earbearing tiller height, perennial habit is strong, and is disease-resistant, quality better, easily and wheat hybridizing solid, had the advantage of common wheat and couchgrass concurrently.
Embodiment
Describe the present invention in detail below in conjunction with specific embodiment, be used for explaining the present invention in this illustrative examples of the present invention and explanation, but not as a limitation of the invention.
Cultivate the perennial wheat of laying down for a short time according to following steps:
(1) phase of surviving the winter is built warm canopy with long fringe couchgrass sowing division, and late Febuary begins to add light with incandescent lamp, and long fringe couchgrass heading is bloomed at the bottom of April;
(2) with after the whole fringe castration of the plant of " short morning eight of Lankao ", transplant to flowerpot, put into long fringe couchgrass temperature canopy, allow its open pollination, every morning 4～6 o'clock and at 6 o'clock in afternoon aim at maternal plant and manually rock long fringe couchgrass, allow its pollen freely be scattered on the maternal column cap, obtain the F of common wheat and the hybridization of long fringe couchgrass
0
At the beginning of (3) 6 months with F
0Tassel dry, by the hybrid combination threshing, preserve, prevent worm food;
At the beginning of (4) 9 months with F
0Planting seed is placed in the illumination box and grows seedlings in sand table;
(5) at the beginning of 10 months by the end of September with F
0Be transplanted in the solarium, tiller, in strong sprout, obtain F
1Seedling.
(6) late November is indoor to F
1Seedling carries out chromosome doubling handles, and makes it become amphidiploid, doubles the back and transplants to the field, strengthens management, and obtains the perennial wheat of laying down for a short time.
During practical operation, adding light time incandescent lamp irradiation area in the above-mentioned steps (1) is 7～9m
2The time of open pollination is more than ten day in the above-mentioned steps (2); Line-spacing is 50～60 centimetres when transplanting in the above-mentioned steps (5), 30～50 centimetres of spacing in the rows; Adopt colchicine chromosome doubling method when chromosome doubling is handled in the above-mentioned steps (6).
More than technical scheme that the embodiment of the invention is provided be described in detail, used specific case herein principle and the embodiment of the embodiment of the invention are set forth, the explanation of above embodiment only is applicable to the principle that helps to understand the embodiment of the invention; Simultaneously, for the those skilled in the art in ability city, according to the embodiment of the invention, the part that on embodiment and range of application, all can change, in sum, this description should not be construed as limitation of the present invention.
Claims (5)
1. the breeding method of the perennial wheat of laying down for a short time is characterized in that may further comprise the steps:
(1) phase of surviving the winter is built warm canopy with long fringe couchgrass sowing division, and late Febuary begins to add light with incandescent lamp, and long fringe couchgrass heading is bloomed at the bottom of April;
(2) with after the whole fringe castration of the plant of " short morning eight of Lankao ", transplant to flowerpot, put into long fringe couchgrass temperature canopy, allow its open pollination, every morning 4～6 o'clock and at 6 o'clock in afternoon aim at maternal plant and manually rock long fringe couchgrass, allow its pollen freely be scattered on the maternal column cap, obtain the F of common wheat and the hybridization of long fringe couchgrass
0
At the beginning of (3) 6 months with F
0Tassel dry, by the hybrid combination threshing, preserve, prevent worm food;
At the beginning of (4) 9 months with F
0Planting seed is placed in the illumination box and grows seedlings in sand table;
(5) at the beginning of 10 months by the end of September with F
0Be transplanted in the solarium, tiller, in strong sprout, obtain F
1Seedling;
(6) late November is indoor to F
1Seedling carries out chromosome doubling handles, and makes it become amphidiploid, doubles the back and transplants to the field, strengthens management, and obtains the perennial wheat of laying down for a short time.
2. the breeding method of the perennial wheat of laying down for a short time according to claim 1 is characterized in that: adding light time incandescent lamp irradiation area in the described step (1) is 7～9m
2
3. the breeding method of the perennial wheat of laying down for a short time according to claim 1 is characterized in that: the time of open pollination is more than ten day in the described step (2).
4. the breeding method of the perennial wheat of laying down for a short time according to claim 1 is characterized in that: line-spacing is 50～60 centimetres when transplanting in the described step (5), 30～50 centimetres of spacing in the rows.
5. the breeding method of the perennial wheat of laying down for a short time according to claim 1 is characterized in that: adopt colchicine chromosome doubling method when chromosome doubling is handled in the described step (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010236197 CN102334414B (en) | 2010-07-23 | 2010-07-23 | Culture method for perennial trititrigia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010236197 CN102334414B (en) | 2010-07-23 | 2010-07-23 | Culture method for perennial trititrigia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102334414A CN102334414A (en) | 2012-02-01 |
| CN102334414B true CN102334414B (en) | 2013-07-03 |
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|---|---|---|---|
| CN 201010236197 Active CN102334414B (en) | 2010-07-23 | 2010-07-23 | Culture method for perennial trititrigia |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646651A (en) * | 2017-10-13 | 2018-02-02 | 山东农业大学 | A kind of cultural method of wheat seedling |
| CN111512731B (en) * | 2020-05-07 | 2021-10-01 | 北京市农林科学院 | Method for promoting germination of elytrigia elongata seeds |
| CN114902916A (en) * | 2022-05-17 | 2022-08-16 | 中国科学院遗传与发育生物学研究所 | Mechanical vegetative propagation method of elytrigia elongata |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601368A (en) * | 2008-06-10 | 2009-12-16 | 沈天民 | Improve the method for wheat and couchgrass cross-fertile rate |
| CN101755683A (en) * | 2008-12-19 | 2010-06-30 | 李祥 | Applications of Elytrigia elongata to genetic improvement of wheat |
-
2010
- 2010-07-23 CN CN 201010236197 patent/CN102334414B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601368A (en) * | 2008-06-10 | 2009-12-16 | 沈天民 | Improve the method for wheat and couchgrass cross-fertile rate |
| CN101755683A (en) * | 2008-12-19 | 2010-06-30 | 李祥 | Applications of Elytrigia elongata to genetic improvement of wheat |
Non-Patent Citations (7)
| Title |
|---|
| 八倍体小偃麦与普通小麦杂交后代花药培养的研究;王洪刚等,1;《西北植物学报》;19930615;第13卷(第03期);186-191 * |
| 刘翠云,1.小麦与长穗偃麦杂种花药培养试验.《陕西农业科学》.1984,(第06期),29-31. |
| 小麦与长穗偃麦杂种花药培养试验;刘翠云,1;《陕西农业科学》;19840629(第06期);29-31 * |
| 李振声 等.(二)杂种染色体加倍.《小麦远缘杂交》.1985,57-61,24-25. * |
| 王洪刚等,1.八倍体小偃麦与普通小麦杂交后代花药培养的研究.《西北植物学报》.1993,第13卷(第03期),186-191. |
| 钟冠昌等,1.长穗偃麦草细胞质小麦核质杂种农艺性状的研究.《西北植物学报》.1993,第13卷(第04期),255-259. |
| 长穗偃麦草细胞质小麦核质杂种农艺性状的研究;钟冠昌等,1;《西北植物学报》;19930815;第13卷(第04期);255-259 * |
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| CN102334414A (en) | 2012-02-01 |
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Effective date of registration: 20161209 Address after: State Road 310 North District 475300 Lankao County Industrial Agglomeration in Henan Province Patentee after: Henan Tianmin Seed Co. Ltd. Address before: Cemetery Road Chengguan Town, Lankao County, Henan province 475330 alley 1 Patentee before: Shen Tianmin |