CN102668975A - Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker - Google Patents
Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker Download PDFInfo
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- CN102668975A CN102668975A CN2012101915121A CN201210191512A CN102668975A CN 102668975 A CN102668975 A CN 102668975A CN 2012101915121 A CN2012101915121 A CN 2012101915121A CN 201210191512 A CN201210191512 A CN 201210191512A CN 102668975 A CN102668975 A CN 102668975A
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- purple leaf
- sterile line
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Abstract
The invention discloses a method for producing a seed by using a three-line hybrid rice sterile line with a purple leaf marker, comprising the following steps of: (1) arranging the sowing time according to the leaf difference and the time difference of the sterile line and the selected restoring line; (2) raising rice seedlings by using a rice seedling bed; (3) arranging an impurity removal work for a female parent rice field in a rice seedling period, and carrying out water and fertilizer management for a male parent and the female parent, wherein a female parent belongs to the sterile line of a purple leaf, therefore, plants with green leaves in the female parent rice field are needed to be pulled up; (4) enabling the ratio of female parent rows to male parent rows in a seed field to be 2:(12-14) and the number of the male parent rows to be 2 in different sowing times, and transplanting male parent plants into a row shaped like a Chinese character pin; (5) spraying Berelex; (6) carrying out artificial supplementary pollination; (7) isolating to prevent hybrid; (8) strengthening the water and fertilizer management and pest control works for the seed field; and (9) mechanically harvesting the male parent in advance. The method has the advantages of time and labor saving and cost reduction. The sterile line is provided with the purple leaf marker; labor can be saved due to the convenient impurity removal work; and mechanical harvesting is adopted, so that the production cost is reduced.
Description
Technical field
The invention belongs to agricultural technology field, relating to the hybrid rice three of cultivating band purple leaf mark is male sterile line, utilizes this male sterile line to carry out breeding of hybrid rice, and it is pure in production of hybrid seeds process, to come quick removal of impurities to protect through identification leaf look characteristics, improves hybrid purity.
Background technology
Hybrid rice is owing to have tangible hybrid vigour, and its output, resistance, adaptability etc. all are superior to general conventional rice kind, thereby obtains to promote preferably.At present, the year cultivated area of China hybrid rice accounts for the half the of the total cultivated area of paddy rice, and output then accounts for 58% of paddy rice gross yield.Although the hybrid rice development rapidly, crossbreed descends owing to reasons such as self-fertility, weather effect make purity, brings about great losses.The method of identifying hybrid seed purity has the strange land plantation to identify, isozyme electrophoresis is identified and dna molecular marker evaluation etc., and these methods can more accurately be identified hybrid purity, but step is cumbersome, time and effort consuming and cost are higher.With regard to identification of seed purity, it is a kind of directly perceived that development must be thought deeply in raising seed quality aspect in the hybrid seed production process, and easy method is guaranteed the seed quality of hybrid rice.Therefore, breeding man design in the leaf color marker introductive crossing rice sterile line, cultivate the male sterile line of band leaf color marker, reach according to the significant difference of leaf look and conveniently remove selfed seed, mix strain etc., reach simple and easy impurity elimination, identify the target of hybrid purity.The present invention imports purple leaf mark property in the male sterile line according to such thinking, and the hybrid rice three of cultivating purple leaf mark is a male sterile line, and utilizing this male sterile line again is to carry out the production of hybrid seeds with recovering, and produces high-quality hybrid rice seeds.
Summary of the invention
The purpose of this invention is to provide that a kind of to have purple leaf mark hybrid rice three be the selection of male sterile line and utilize this male sterile line and recover the method that system carries out the production of hybrid seeds.
Having purple leaf mark hybrid rice three is the selection of male sterile line:
(1) utilize the rice varieties of purple leaf mark and green leaf paddy rice maintenance line material to hybridize F
1In generation, all show as green; F2 shows as 3: 13 for purple leaf and greenery or separates at 5: 59; The directed offspring who selects again with a plurality of good maintenance lines hybridization, it is good and have the Fertile material of purple leaf mark to select comprehensive proterties at last, as corresponding maintenance line;
(2) with in the step (1) have purple leaf mark Fertile material be male parent and greenery three be male sterile line hybridize F
1, plantation F
1Generation, F
1In generation, select good sterile plant of economical character and male parent to backcross; In the back of backcrossing, select the sterile strain of purple leaf; Through continuous backcross many generations, choosing has purple leaf mark among the offspring, economical character is good and the individual plant of thorough abortion, and finally having selected the paddy rice three with purple leaf mark is male sterile line.
Use the hybrid rice producing method for seed of this male sterile line:
(1) arrange date of seeding properly according to leaf difference and the time difference of male sterile line and selected recovery system, guarantee the parental instinct flower synchronization, arrange male sterile line than to recover system Zao and ear to be advisable about 3 days, male parent will divide for two phases sowed, and I phase male parent was than II phase male parent early sowing 8-10 days.
(2) adopt the paddy field seedling-cultivation plate.Good suitable seedling furrow are played earlier in the paddy field, on the furrow face, spread the seedling dish, and the field mud that layer overlay is very thin on the seedling dish again will pass through this seed of vernalization father and mother and evenly sow and completing on the seedling dish of field mud, press kind, build seedling fertilizer.
(3) a removal of impurities job is arranged in the maternal rice seedling bed of seeding stage, because female parent is a purple leaf male sterile line, therefore will green leaf plant in the maternal rice seedling bed be pulled out, and carries out father and mother's water and fertilizer management originally.
(4) the seed farm female parent is 2 with male parent row ratio: 12-14, and 2 row of male parent behavior different sowing dates, paternal plant is inserted by the article word is capable.
(5) spray " 920 ".Under normal circumstances, the male sterile line heading began spray " 920 " at 5～10% o'clock. about every mu of consumption 10 grams, divide to spray in three days.Add spray male parent 2-3 gram altogether in addition in second and third sky.
(6) artificial supplementary pollination.Catch up with the powder time to be as the criterion should be with male parent the flowers are in blossom loose powder the time, caught up with pollen three times in one day, increase pollen density, improve outcrossing seed-setting rate.
(7) carry out isolation, prevent to mix.Generally apart from not planting the rice varieties of other heading same period in 200 meters of the seed farms or staggering heading stage more than 12 days; This male sterile line has purple leaf mark; In female parent is capable, can remove greenery easily and mix strain, will conscientiously remove the maintenance line that mixes in female parent is capable simultaneously.
(8) water and fertilizer management of reinforcement seed farm, extermination of disease and insect pest work.
(9) cut off male parent ahead of time, adopt mechanical harvesting.After this season pollination finishes, in time cut off male parent ahead of time; Carry out the cleaning works of grain header before the grain header harvesting, get rid of the seed that mixes.
The present invention has following effect
(1) hybrid purity is guaranteed in simple, intuitive removal of impurities, improves seed quality.This male sterile line has purple leaf mark, nursery period maternal field just can remove the assorted strain of greenery easily.As long as carry out in the seed farm the assorted strain of non-male parent greenery and mix the maintenance line removal of impurities work in female parent is capable, prevent mechanical admixture, just can obtain highly purified hybrid.
(2) save labor, practice thrift cost.This male sterile line has purple leaf mark, and removal of impurities work easily can be saved manual work, adds the employing mechanical harvesting, has therefore reduced production cost.
Embodiment
Following examples are used to explain the present invention, are not deviating under the present invention's spirit and the nature, and to the inventive method, the modification that step or condition are done all belongs to scope of the present invention.
Cultivation has the practical implementation process that purple leaf mark three is a male sterile line:
(1) 1994 year early season with the maintenance line intermediate materials K green grass or young crops of the purple leaf kind IR1552 that derives from the International Rice Research Institute and academy of agricultural sciences, Guangxi paddy rice institute oneself wound hybridize F
1Generation, season in evening plantation F
1, F
1Dai Yese all shows as green; Nineteen ninety-five is season plantation F early
2, F
2In generation, isolated purple leaf and green leaf plant, and selecting the good purple leaf plant of economical character is seed plantation offspring.
(2) filial generation of step (1) selected 1 good purple leaf individual plant of proterties and the maintenance line Zhenshan 97B hybridization that derives from Jiangxi in 1972 academy of agricultural sciences's seed selection to obtain hybrid seed in season in evening in 1997 after 3 generations of selfing, 1998 seasons plantation F early
1, F
1All be the greenery plant, mix and receive F
1Seed; Season in evening in 1998 plantation F
2, selecting the plantation of purple leaf plant seedling stage, group receives and plants.
Season (3) 1999 years morning is at the F of step (2) filial generation
3Middle proterties good 1 purple leaf individual plant and academy of agricultural sciences, the Guangxi paddy rice research institute selected obtains crossbreed from the rich B hybridization of the maintenance line of wound, and season in evening in 1999 was planted F
1, F
1Complete is the greenery plant, and group receives and plants; Early season was planted F in 2000
2, selecting the good purple leaf individual plant of proterties, group receives and plants; After 2 generations of selfing, F
4Dai Xuanzi leaf plant group receives and plants.
(4) the purple leaf offspring F in the season evening calendar year 2001 plantation step (3)
5, and from F
5In generation, selected good purple leaf plant 5 strains of proterties and derives from the rich A test cross that Yulin Bobai County, the Guangxi institute of agricultural sciences breeds, and early season was planted backcross progeny B in 2002
1F
1And F
6, B
1F
1Plant is green, from F6, selects 2 strains and B
1F
1Pairing backcrossing; Season in evening in 2002 plantation B
2F
1And F
7, from B
2F
1In select the sterile strain of purple leaf, backcross a pair of for every pair; After 8 generations of backcrossing, season in evening in 2005 plantation B
8F
1And F
13, B wherein
8F
1As the red A of male sterile line called after elder generation, F
13Be the corresponding maintenance line called after red B of elder generation.
The male sterile line that step (4) obtains red A earlier passed through the authorization of the Guangxi kind committee in 2008.
Table 1 is red A, B seed selection pedigree earlier
Utilization has the implementation process that purple leaf marking sterile series carries out the production of hybrid seeds:
Utilizing male sterile line red A of elder generation and greenery to recover is the R7571 production of hybrid seeds
(1) according to male sterile line earlier red A be the leaf difference and time difference of R7571 to arrange date of seeding properly with recovering.The plantation in Nanning, the early March sowing, red A stem blade 13 leaves of elder generation, sowing to the fringe that begins lasted about 80 days; Sowing mid-July in season evening, sowing to the fringe that begins lasts 60 days.Recovery is the plantation of R7571 Nanning, sowing at the beginning of 3 months, and stem blade 16 leaves, sowing to the fringe that begins lasts 90 days, sowing mid-July in season evening, sowing to the fringe that begins lasts 80 days.Male sterile line red A earlier is that R7571 heading in 3 days ahead of time is more suitable than recovering.Therefore, the red A of male sterile line elder generation is than R7571 sowing in late about 13 days.R7571 divides two phases sowings, I phase and II phase to be separated by to be advisable in 5-8 days.
(2) adopt the paddy field seedling-cultivation plate.Good seedling is played in the paddy field earlier, on face, spreads the seedling dish, and the field mud that layer overlay is very thin on the seedling dish again will pass through vernalization red A of elder generation or R7571 seed and evenly be sprinkled upon on the seedling dish of completing field mud.
(3) a removal of impurities job of the maternal neoasozin row of seeding stage because female parent is a purple leaf male sterile line, therefore will be pulled out maternal Tanaka's green leaf plant, carries out father and mother's water and fertilizer management originally, extermination of disease and insect pest work.
(4) the red A of seed farm elder generation is 2 with the capable ratio of R7571: 12-14, male parent row R7571 are 2 row of different sowing dates, by the capable arrangement of article word.
(5) rationally spray " 920 ".Under normal circumstances, earlier red A heading began spray " 920 " at 5～10% o'clock. about every mu of consumption 10 grams, divide to spray in three days.Respectively add spray male parent 2-3 gram in addition in second and third sky.
(6) artificial supplementary pollination.Catch up with the powder time to be as the criterion should be with male parent R7571 the flowers are in blossom loose powder the time, caught up with pollen three times in one day, increase pollen density, improve outcrossing seed-setting rate.
(7) carry out isolation, removal of impurities work.Generally apart from originally staggering more than 12 days at the rice varieties of not planting other heading same period in 200 meters of the seed farms or heading stage and father and mother; This male sterile line has purple leaf mark; In female parent is capable, can remove greenery easily and mix strain, but the assorted strain that will conscientiously remove the non-male parent of greenery in the maintenance line that mixes in the capable red A earlier of female parent and the seed farm.
(8) water and fertilizer management of reinforcement seed farm, extermination of disease and insect pest work.
(9) in time slice off male parent, adopt mechanical harvesting.This season pollination finishes, and in time cuts off male parent earlier.Carry out the cleaning works of grain header before the grain header harvesting, get rid of the seed that mixes.
Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (1)
1. to have purple leaf mark hybrid rice three be the method for sterile lines seed making in an application, it is characterized in that, may further comprise the steps:
(1) arrange date of seeding properly according to leaf difference and the time difference of this male sterile line and selected recovery system, guarantee the parental instinct flower synchronization, arrange male sterile line than to recover system Zao and ear to be advisable about 3 days, male parent will divide for two phases sowed, and I phase male parent was than II phase male parent early sowing 8-10 days;
(2) adopt the paddy field seedling-cultivation plate.Good suitable seedling furrow are played earlier in the paddy field, on the furrow face, spread the seedling dish, and the field mud that layer overlay is very thin on the seedling dish again will pass through this seed of vernalization father and mother and evenly sow and completing on the seedling dish of field mud, press kind, build seedling fertilizer;
(3) a removal of impurities job is arranged in the maternal rice seedling bed of seeding stage, because female parent is a purple leaf male sterile line, therefore will green leaf plant in the maternal rice seedling bed be pulled out, and carries out father and mother's water and fertilizer management originally;
(4) the seed farm female parent is 2 with male parent row ratio: 12-14, and 2 row of male parent behavior different sowing dates, paternal plant is inserted by the article word is capable.；
(5) spray " 920 ".Under normal circumstances, the male sterile line heading began spray " 920 " at 5～10% o'clock. about every mu of consumption 10 grams, divide to spray in three days.Add spray male parent 2-3 gram altogether in addition in second and third sky;
(6) artificial supplementary pollination.Catch up with the powder time to be as the criterion should be with male parent the flowers are in blossom loose powder the time, caught up with pollen three times in one day, increase pollen density, improve outcrossing seed-setting rate;
(7) carry out isolation, prevent to mix.Generally apart from not planting the rice varieties of other heading same period in 200 meters of the seed farms or staggering heading stage more than 12 days; This male sterile line has purple leaf mark; In female parent is capable, can remove greenery easily and mix strain, will conscientiously remove the maintenance line that mixes in female parent is capable simultaneously;
(8) water and fertilizer management of reinforcement seed farm, extermination of disease and insect pest work;
(9) cut off male parent ahead of time, adopt mechanical harvesting.After this season pollination finishes, in time cut off male parent ahead of time; Carry out the cleaning works of grain header before the grain header harvesting, get rid of the seed that mixes;
It is said that to have purple leaf mark hybrid rice three be the selection of male sterile line:
A: utilize the rice varieties and the hybridization of green leaf paddy rice maintenance line material of purple leaf mark, F
1In generation, all show as green; F2 shows as 3: 13 for purple leaf and greenery or separates at 5: 59; The directed offspring who selects again with a plurality of good maintenance lines hybridization, it is good and have the Fertile material of purple leaf mark to select comprehensive proterties at last, as corresponding maintenance line;
B: with in the steps A have purple leaf mark Fertile material be male parent and greenery three be male sterile line hybridize F
1, plantation F
1Generation, F
1In generation, select good sterile plant of economical character and male parent to backcross; In the back of backcrossing, select the sterile strain of purple leaf; Through continuous backcross many generations, choosing has purple leaf mark among the offspring, economical character is good and the individual plant of thorough abortion, and finally having selected the paddy rice three with purple leaf mark is male sterile line.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101915121A CN102668975A (en) | 2012-06-11 | 2012-06-11 | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker |
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|---|---|---|---|
| CN2012101915121A CN102668975A (en) | 2012-06-11 | 2012-06-11 | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105265261A (en) * | 2015-10-16 | 2016-01-27 | 苏州市农业科学院 | Test cross parent field arranging and transplanting method |
| CN106718989A (en) * | 2016-11-11 | 2017-05-31 | 安徽华安种业有限责任公司 | A kind of producing method for seed of hybrid rice |
| CN109042292A (en) * | 2018-08-03 | 2018-12-21 | 武汉大学 | A kind of hybrid rice seed breeding process of same period rice transplanting parent |
| CN113475385A (en) * | 2021-07-20 | 2021-10-08 | 贺州市农业科学院 | Breeding method of sterile line of three-line hybrid rice with leaf marking property |
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|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| CN1709037A (en) * | 2005-06-14 | 2005-12-21 | 浙江大学 | Monitorable rice planting method |
| CN1799333A (en) * | 2005-12-27 | 2006-07-12 | 辽宁省稻作研究所 | High-yield seeds production technique for japonica hybrid rice |
-
2012
- 2012-06-11 CN CN2012101915121A patent/CN102668975A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1185898A (en) * | 1997-09-10 | 1998-07-01 | 武汉大学 | Selection of sterility lined with leaf marks for three way crossed rice |
| CN1709037A (en) * | 2005-06-14 | 2005-12-21 | 浙江大学 | Monitorable rice planting method |
| CN1799333A (en) * | 2005-12-27 | 2006-07-12 | 辽宁省稻作研究所 | High-yield seeds production technique for japonica hybrid rice |
Non-Patent Citations (3)
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|---|
| 邓国富等: "水稻紫红叶标记不育系先红A 的选育及应用", 《中国稻米》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105265261A (en) * | 2015-10-16 | 2016-01-27 | 苏州市农业科学院 | Test cross parent field arranging and transplanting method |
| CN105265261B (en) * | 2015-10-16 | 2018-01-12 | 苏州市农业科学院 | The field arrangement of tester a kind of and method for transplanting |
| CN106718989A (en) * | 2016-11-11 | 2017-05-31 | 安徽华安种业有限责任公司 | A kind of producing method for seed of hybrid rice |
| CN109042292A (en) * | 2018-08-03 | 2018-12-21 | 武汉大学 | A kind of hybrid rice seed breeding process of same period rice transplanting parent |
| CN109042292B (en) * | 2018-08-03 | 2021-12-28 | 武汉大学 | Hybrid rice seed production method for parents of rice transplanting at same period |
| CN113475385A (en) * | 2021-07-20 | 2021-10-08 | 贺州市农业科学院 | Breeding method of sterile line of three-line hybrid rice with leaf marking property |
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Application publication date: 20120919 |