CN110100722B - Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety - Google Patents
Breeding method of purple-leaf white-flower cabbage type landscape rape conventional variety Download PDFInfo
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- CN110100722B CN110100722B CN201910486526.8A CN201910486526A CN110100722B CN 110100722 B CN110100722 B CN 110100722B CN 201910486526 A CN201910486526 A CN 201910486526A CN 110100722 B CN110100722 B CN 110100722B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a breeding method of a conventional variety of purple-leaf white-flower cabbage type landscape rape. Comprises the following steps: a) hybridizing the brassica napus strain with the brassica napus as a male parent and the brassica napus strain with green leaves and white flowers as a female parent to obtain hybrid progeny F1(ii) a b) Subjecting said hybrid progeny F1Selfing to obtain F2Plant growing; c) selecting the F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and 3 times of backcross are carried out to obtain backcross progeny BC3F1Plant growing; d) subjecting the backcross progeny BC3F1Plants are selfed to obtain BC3F2Plant generation, selecting BC3F2And (3) generating a plant line with purplish seedling leaves, whitest flowering flowers and highest yield in the plant generation, and obtaining the purple-leaf white-flower cabbage type conventional rape variety. The method can be used for breeding the conventional varieties of the purple-leaf white-flower cabbage type landscape rape.
Description
Technical Field
The invention belongs to the field of rape breeding, and relates to a breeding method of a conventional variety of purple-leaf white-flower cabbage type landscape rape.
Background
Oilseed rape is a widely cultivated oil crop worldwide. China is a big rape producing country, the planting area is about 1 hundred million mu throughout the year, the area and the yield are at the top of the world, but in recent years, the area and the total yield of rape slide down, the low comparative benefit of rape planting is the main reason, the improvement of the comparative benefit of rape planting is needed, and the development of multifunctional rape utilization is one of effective measures.
The rape has six functions of oil use, flower use, honey use, vegetable use, feed use, fertilizer use and the like, wherein the rape flower use is mainly to cultivate the rape as an ornamental crop for appreciation, but the rape is used as a landscape crop, particularly used for rape flower sea with landscape pattern design, and the rape flower sea can only appreciate patterns and flower sea in the flowering phase and cannot attract tourists in the seedling phase. Therefore, the breeding of the landscape rape which can observe leaves and flowers in the seedling stage has important significance for developing the multifunctional utilization of the rape.
Disclosure of Invention
The invention aims to provide a breeding method of a conventional variety of purple-leaf white-flower cabbage type landscape rape.
The method provided by the invention is to transfer the purple leaf character of purple green vegetables into a green leaf and white flower cabbage type rape line and breed and obtain the purple leaf and white flower cabbage type conventional rape variety.
In the above method, the brassica napus var viridis strain is ZH 17;
the ZH17 is obtained by selfing and breeding after the hybridization of Zhongshuang 11 and Baihuashanghai oil 17;
the preservation number of the Baihuashanyou 17 is CGMCC No. 17384.
In the method, the method for transferring the purple leaf character of the purple cabbage into the green-leaf white-flower cabbage type rape line comprises the following steps:
a) hybridizing the brassica napus strain with the brassica napus as a male parent and the brassica napus strain with green leaves and white flowers as a female parent to obtain hybrid progeny F1；
b) Subjecting said hybrid progeny F1Selfing to obtain F2Plant growing;
c) selectingSaid F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and 3 times of backcross are carried out to obtain backcross progeny BC3F1Plant growing;
the 3 backcrosses are all single plants which are selected from the progeny of the previous backcross and have the purest leaf in the seedling stage and the whitest flower in the flowering stage for selfing, and then the single plants which are selected from the progeny of the selfing and have the purest leaf in the seedling stage and the whitest flower in the flowering stage are used as non-recurrent parents for the next backcross;
d) selecting the backcross progeny BC3F1Selfing the single plant with purple seedling leaves and white flowering flowers to obtain BC3F2Plant generation, selecting BC3F2And (3) generating a plant line with purplish seedling leaves, whitest flowering flowers and highest yield in the plant generation, and obtaining the purple-leaf white-flower cabbage type conventional rape variety.
In the above method, the 3 backcrosses include the following steps:
c-1, selecting the F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and backcross is carried out for 1 time to obtain backcross progeny BC1F1Plant growing; then the back-cross progeny BC1F1Selecting the single plant with purple seedling leaves and white flower flowers from the plants to self-breed to obtain BC1F2Plant growing;
c-2, selecting the BC1F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and backcross is carried out for 2 times to obtain backcross progeny BC2F1Plant growing; then the back-cross progeny BC2F1The plants are selected to be inbred with the single plant with purple seedling leaves and white flowering flowers to obtain BC2F2Plant growing;
c-3, selecting the BC2F2In the plants, the single plant with purple seedling leaves and white flower flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and backcross is carried out for 3 times to obtain backcross progeny BC3F1And (5) plant growing.
In the above method, c-1), the F is selected2The number of the single plants which have the purest leaf in the seedling stage and the whitest flower in the flowering stage and are non-recurrent parent is 10;
or, in c-2) to c-3), the number of the single plants which are selected from the backcross progeny plants as non-recurrent parents and have the purple seedling leaves and the whitest flowering flowers is 10;
or, in c-1) to c-3), the number of the single plants which are used for selfing and have the purple leaf at the seedling stage and the whitest flower at the flowering stage is 2 in each time of selection from backcross progeny.
In the method, in d), the backcross progeny BC is selected3F1The number of individuals with the purest leaf at seedling stage and the whitest flower at flowering stage in the plants was 10.
In the method, the sites of hybridization, selfing, backcrossing and selfing are all staggered.
The application of the green-leaf white-flowered cabbage type rape strain ZH17 in the conventional variety breeding of purple-leaf white-flowered cabbage type landscape rape is also within the protection range of the invention;
the ZH17 is obtained by selfing and breeding after the hybridization of Zhongshuang 11 and Baihuashanghai oil 17;
the preservation number of the Baihuashanyou 17 is CGMCC No. 17384.
In the method, the white-flowered cabbage type rape line is a cabbage type conventional rape line white-flowered ZH17, the white-flowered ZH17 is formed by hybridizing a Zhongshuang 11 (bred by oil crop research institute of Chinese academy of agricultural sciences, national oil 20008030) female parent and a male parent Huyou 17 (bred by crops of Shanghai academy of agricultural sciences, national oil 2011006) white-flowered mutant strain 'white-flowered Huyou 17', bagging and selfing for 5 continuous generations, and carrying out systematic breeding by taking visual white-flowered as target characters.
Baihuashanyou oil 17 with preservation number of CGMCC No.17384, China Committee for culture Collection of microorganisms, CGMCC for short, address: the microbial research institute of Chinese academy of sciences, No. 3 Xilu No.1 of Beijing, Chao code 100101, has a storage date of 2019, 2 months and 28 days, and is classified and named as Brassica napus.
The test of the invention proves that the purple leaf character of purple green vegetables can be transferred into the white cabbage type rape line through hybridization and backcross technology, and excellent conventional rape varieties with purple leaf and white flower characters (purple leaves in seedling stage and white flowers in full-bloom stage) are bred as multifunctional landscape rape for leaf observation and flower observation in seedling stage and oil extraction of grains; when the landscape rape variety with purple seedlings and white flowers in full-bloom stage and the common rape with green leaves and yellow flowers are used for rape flowering sea with landscape pattern design, tourists can be attracted in the seedling stage and the flowering stage, the cultivation time of the rape as a landscape crop is prolonged, and the income is increased.
Drawings
FIG. 1 is a process diagram of the conventional variety purple oil No.1 of purple-leaf white-flower cabbage type landscape rape.
FIG. 2 shows purple cabbage.
FIG. 3 is a comparison of purple leaf white cabbage type landscape rape and green leaf cabbage type rape leaves.
FIG. 4 shows the seedling stage comparison of purple-leaf white-flower cabbage type landscape rape and green-leaf cabbage type rape (left, white-flower ZH 17; right, purple oil No. 1).
FIG. 5 is a comparison of the bud stages of purple-leaf and white-flower cabbage type landscape rape and green-leaf cabbage type rape (left, purple oil No. 1; right, white-flower ZH 17).
FIG. 6 is the bolting period of purple-leaf white-cabbage type landscape rape.
FIG. 7 is the inflorescence of purple-leaf white-flower cabbage type landscape rape.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 Breeding of conventional varieties of purple-leaf white-flower cabbage type landscape rape
First, the breeding of cabbage type conventional rape line white flower ZH17
White flower ZH 17: the Chinese-Shuang 11 (bred by oil crop research institute of Chinese agricultural academy of sciences, national oil inspection 20008030) is used as female parent to hybridize with the male parent Huyou 17 (bred by crops of Shanghai academy of sciences, national oil inspection 2011006) white flower mutant strain "Baihua Huyou 17", then the hybrid is continuously bagged for 5 generations for selfing, and the hybrid is systematically bred by taking visual white flower as target character.
Baihuashanyou oil 17 with preservation number of CGMCC No.17384, China Committee for culture Collection of microorganisms, CGMCC for short, address: the microbial research institute of Chinese academy of sciences, No. 3 Xilu No.1 of Beijing, Chao code 100101, has a storage date of 2019, 2 months and 28 days, and is classified and named as Brassica napus.
Second, cross transformation
In 3 months in 2010, cross-breeding was performed according to FIG. 1:
1. hybridization of
In 3 months of 2010, 2 purple greens (Fangzhen purple greens, product number: 7095, Lichonghui county, Lichongfeng breed; fig. 2) are used as male parents and 5 white cabbage type rape lines, white flower ZH17, are used as female parents for hybridization to obtain 10 hybrid progeny F1Seeds;
2. selfing
In 5 months 2010, 10 portions of F are harvested1The hybrid seeds are brought to Qinghai, 10 parts of F in 7 months in 20101Selecting 2 single plants with purple leaves and white flowers in seedling stage, bagging and selfing to obtain 20F plants2Selfing seeds;
3. multiple backcrosses
1) First backcross
In 9 months in 2010, 20F plants harvested from the combined fertilizer planting Qinghai2Selfed seed, each plant F22 lines of inbred plants, 20F in total2And (5) strain. 2011 20F in 3 months2Selecting 10 single plants with purple seedling leaves and white flower flowers in the flowering phase from the strains; with 10 strains F2Backcrossing with 10 white flower ZH17 as male parent (non-recurrent parent) and 10 backcross generations 1 generation of colony BC1F1Seeds;
in 5 months of 2011, 10 harvested backcrosses are carried out on 1 generation population BC1F1The seeds are brought to Qinghai for planting to obtain 10 parts of BC1F1Plant growing; from 10 parts of BC in 7 months in 20111F1Selecting the single plant with purple seedling leaves and white flower in flowering phaseBagging 2 plants for selfing to obtain 20 selfed plant seeds, and recording the seeds as BC1F2。
2011 in 9 months, 20 BC harvested from Qinghai are planted in a fertilizer1F2Seed to obtain BC1F2Plants, 2 rows planted per plant, 20 BC in total1F2And (5) strain.
2) The 2 nd backcross
From 20 BC 3 months 20121F2Selecting 10 single plants with purple seedling leaves and white flower flowers in the flowering phase from the strains; the 10 BC plants1F2Backcrossing with 10 white flower ZH17 as male parent (non-recurrent parent) and 10 backcross generations 2 of colony BC2F1Seeds;
5 months 2012, 10 BC harvested2F1The seeds are brought to the Qinghai for planting to obtain BC2F1Plant, 10 BC from 7 months 20122F1Selecting 2 single plants with purple leaves and white flowers in seedling stage from the plants, bagging and selfing to obtain 20 selfed plant seeds, and recording the seeds as BC2F2；
In 9 months of 2012, 20 BC harvested from combined fertilizer planted Qinghai2F2Seed to obtain BC2F2Plants, 2 rows planted per plant, 20 BC in total2F2And (5) strain.
3) 3 rd backcross
20 BC in 3 months in 20132F2Selecting 10 single plants with purple seedling leaves and white flower flowers in the flowering phase from the strains; the 10 BC plants2F2Backcrossing with 10 white flower ZH17 as male parent (non-recurrent parent) and 10 backcrossed 3-generation population BC3F1Seeds;
in 5 months in 2013, 10 portions of BC harvested3F1The seeds are brought to the Qinghai for planting to obtain BC3F1Plant, 10 parts of BC in 7 months in 20133F1Selecting 10 individual plants with purple seedling leaves and white flower flowers in the flowering period from the plants, bagging and selfing to obtain 10 selfed plants, and recording the 10 selfed plants as BC3F2Seeds;
9 months in 2013, Hehe10 parts of BC harvested from fertilizer planted Qinghai3F2Seed to obtain BC3F2Plants were planted in 100 plants each, for a total of 10 lines.
10 BC in 3 months in 20143F2The plant lines are separately propagated by using 100-mesh nylon net accounts before the initial flowering, the purple-leaf white-flower characters of 10 lines separately propagated by the net accounts are investigated in the mature period, the yield is measured after the rape is mature, and the line with purple leaves at the seedling stage, the white flowers at the flowering stage and the highest yield is screened as the target purple-leaf white-flower cabbage type landscape rape conventional variety, which is named as purple oil No. 1.
FIG. 3 is a comparison of purple leaf white cabbage type landscape rape and green leaf cabbage type rape leaves.
The results of comparing the seedling stage of common green cabbage type rape (white flower ZH17) with red cabbage and white flower landscape rape (purple oil No. 1) are shown in fig. 4. The bud stage comparison of common green cabbage type rape (white flower ZH17) and purple white flower cabbage landscape rape (purple oil No. 1) is shown in FIG. 5. It can be seen that the purple leaf character of the purple leaf white flower rape (right, purple oil No. 1) is obvious and consistent by visual inspection at the seedling stage. FIG. 6 is the bolting period of purple-leaf white-cabbage type landscape rape. FIG. 7 is the inflorescence of purple-leaf white-flower cabbage type landscape rape.
Claims (6)
1. A method for breeding a conventional variety of purple-leaf white-flower cabbage type landscape rape comprises transferring purple-leaf characters of purple green vegetables into a green-leaf white-flower cabbage type rape line, and breeding to obtain a conventional variety of purple-leaf white-flower cabbage type rape;
the green-leaf white-flower cabbage type rape strain is ZH 17;
the ZH17 is obtained by selfing and breeding after the hybridization of Zhongshuang 11 and Baihuashanghai oil 17;
the preservation number of the Baihuashanyou 17 is CGMCC No. 17384;
the method for transferring the purple leaf character of the purple cabbage into the green-leaf white-flower cabbage type rape line comprises the following steps:
a) hybridizing the brassica napus strain with the brassica napus as a male parent and the brassica napus strain with green leaves and white flowers as a female parent to obtain hybrid progeny F1；
b) Will be provided withThe hybrid progeny F1Selfing to obtain F2Plant growing;
c) selecting the F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and 3 times of backcross are carried out to obtain backcross progeny BC3F1Plant growing;
the 3 backcrosses are all single plants which are selected from the progeny of the previous backcross and have the purest leaf in the seedling stage and the whitest flower in the flowering stage for selfing, and then the single plants which are selected from the progeny of the selfing and have the purest leaf in the seedling stage and the whitest flower in the flowering stage are used as non-recurrent parents for the next backcross;
d) selecting the backcross progeny BC3F1Selfing the single plant with purple seedling leaves and white flowering flowers to obtain BC3F2Plant generation, selecting BC3F2And (3) generating a plant line with purplish seedling leaves, whitest flowering flowers and highest yield in the plant generation, and obtaining the purple-leaf white-flower cabbage type conventional rape variety.
2. The method of claim 1, wherein:
the 3 backcrosses include the following:
c-1, selecting the F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and backcross is carried out for 1 time to obtain backcross progeny BC1F1Plant growing; then the back-cross progeny BC1F1Selecting the single plant with purple seedling leaves and white flower flowers from the plants to self-breed to obtain BC1F2Plant growing;
c-2, selecting the BC1F2In the plants, the single plant with the purple seedling leaves and the white flowering flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and backcross is carried out for 2 times to obtain backcross progeny BC2F1Plant growing; then the back-cross progeny BC2F1The plants are selected to be inbred with the single plant with purple seedling leaves and white flowering flowers to obtain BC2F2Plant growing;
c-3, selecting the BC2F2In the plants, the single plant with purple seedling leaves and white flower flowers is taken as a non-recurrent parent, the green-leaf white-flower cabbage type rape line is taken as a recurrent parent, and backcross is carried out for 3 times to obtain backcross progeny BC3F1And (5) plant growing.
3. The method of claim 2, wherein:
c-1), selecting the F2The number of the single plants which have the purest leaf in the seedling stage and the whitest flower in the flowering stage and are non-recurrent parent is 10;
or, in c-2) to c-3), the number of the single plants which are selected from the backcross progeny plants as non-recurrent parents and have the purple seedling leaves and the whitest flowering flowers is 10;
or, in c-1) to c-3), the number of the single plants which are used for selfing and have the purple leaf at the seedling stage and the whitest flower at the flowering stage is 2 in each time of selection from backcross progeny.
4. A method according to any one of claims 1-3, characterized in that:
d) selecting the backcross progeny BC3F1The number of individuals with the purest leaf at seedling stage and the whitest flower at flowering stage in the plants was 10.
5. A method according to any one of claims 1-3, characterized in that: in the method, the sites of hybridization, selfing, backcrossing and selfing are all staggered.
6. The application of the green-leaf white-flowered cabbage type rape strain ZH17 in the conventional variety breeding of purple-leaf white-flowered cabbage type landscape rape;
the ZH17 is obtained by selfing and breeding after the hybridization of Zhongshuang 11 and Baihuashanghai oil 17;
the preservation number of the Baihuashanyou 17 is CGMCC No. 17384.
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| CN111512959A (en) * | 2020-05-22 | 2020-08-11 | 汉中职业技术学院 | Hybrid rape breeding method capable of early identifying seed purity |
| CN112438199B (en) * | 2020-12-18 | 2021-11-02 | 湖南省作物研究所 | Breeding method of rape variety suitable for balcony cultivation |
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| CN105766626A (en) * | 2016-05-12 | 2016-07-20 | 安徽农业大学 | Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination |
| CN105900831A (en) * | 2016-05-12 | 2016-08-31 | 安徽农业大学 | Breeding method of main-inflorescence multi-silique brassica napus type conventional rape variety |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105766626A (en) * | 2016-05-12 | 2016-07-20 | 安徽农业大学 | Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination |
| CN105900831A (en) * | 2016-05-12 | 2016-08-31 | 安徽农业大学 | Breeding method of main-inflorescence multi-silique brassica napus type conventional rape variety |
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