CN107360966A - Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high selection and propagation method - Google Patents
Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high selection and propagation method Download PDFInfo
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- CN107360966A CN107360966A CN201710767781.0A CN201710767781A CN107360966A CN 107360966 A CN107360966 A CN 107360966A CN 201710767781 A CN201710767781 A CN 201710767781A CN 107360966 A CN107360966 A CN 107360966A
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- river
- spring river
- exposing ratio
- rice
- stigma exposing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses it is a kind of colored when early, Xian round-grained rice osculant sterile line spring river 35A that stigma exposing ratio is high selection and reproduction technique, belong to rice heterosis utilization field.The breeding method of the present invention comprises the following steps：From with characteristics such as stigma exposing ratio height, Hua Shizao, anti-stripe virus diseases mix indica type late japonica rice maintainer spring river 19B and indica maintainer spring river 23B with the characteristic such as stigma exposing ratio is high, Hua Shite is early hybridizes, it is returned again using the river 16A of late japonica rice sterile spring with characteristics such as stigma exposing ratio high, Hua Shite morning as male parent, plantation backcross progeny to B6F1Algebraically is relatively low, has stablized, and is named as spring river 35A.It is early during the Xian round-grained rice osculant sterile line spring river 35A flowers of this method seed selection, stigma exposing ratio is high, outcrossing seed-setting rate is high, strong stress resistance (specific), best in quality.
Description
Technical field
The present invention relates to a kind of Yunnan I types Xian round-grained rice osculant sterile line breeding methods, when particularly a kind of colored outside early, column cap
The high Xian round-grained rice osculant sterile line spring river 35A of dew rate breeding and propagation method.
Background technology
The japonica rice variety on the glutinous rice zone of the provinces and cities such as Jiangsu, Anhui, Zhejiang, Shanghai and Xian round-grained rice companion plantings area, production in recent years
Stripe virus disease occur to aggravate year by year, therefore, the resistance that the seed selection of Japonica Hybrid Combination nova must pay attention to stripe virus disease is asked
Topic, the japonica rice sterile of the anti-stripe virus disease of transformation is as one of key of Japonica Hybrid stripe virus disease resistance breeding.
For Japonica Hybrid Combination nova in addition to itself should possess excellent Comprehensive Traits, the height of hybrid seed yield is also to influence
An important factor for can it be widely applied.The major part three that China applies in production at present is japonica rice sterile, general column cap
Not exposed or exposing ratio is slower when relatively low, colored, and outcrossing seed-setting rate and hybrid seed yield are relatively low, and this constrains hybridization to a certain extent
The popularization and application of japonica rice Combination nova, there is an urgent need to the japonica rice sterile that transformation goes out high outcrossing seed-setting rate, to improve hybrid seed yield.
In addition, the japonica rice sterile in Zhejiang Province is mainly based on late round-grained rice type at present, such as peaceful 67A, river in Zhejiang Province round-grained rice No. 2 A, Chun Jiang
The sterile lines such as 12A, elegant water 110A, all it is the sterile line of late round-grained rice type, is limited by sterile line photonasty is stronger, matches somebody with somebody hybridization round-grained rice
The photonasty of rice or ideotype breeding combination is also relatively strong, some combinations even time of infertility it is long and can not be in Zhejiang Province
Utilize.Therefore, the Japonica Hybrid or ideotype breeding combination that the seed selection time of infertility is shorter it may first have to abundant japonica rice
The ripe phase type of sterile line, as seed selection photonasty is weaker, the japonica rice sterile of temperature-sensitive type, consider life in configuration Hybrid Rice Combination
Just there is greater flexibility and initiative when educating the phase.
Therefore, cultivate that Hua Shizao, stigma exposing ratio be high and the middle round-grained rice sterile line of strong stress resistance, for improve hybrid seed yield and
It is significant to promote Japonica Hybrid Combination nova.
The content of the invention
It is an object of the invention to provide it is a kind of colored when early, stigma exposing ratio is high and the middle round-grained rice sterile line spring river of strong stress resistance
35A breeding and propagation method, so as to cultivate Hua Shizao, stigma exposing ratio height and strong stress resistance middle round-grained rice sterile line, it is used for
Improve Hybrid Rice Combination hybrid seed yield and the popularization and application in production.
In order to realize above-mentioned purpose, present invention employs following technical scheme：
Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high selection, this method include
Following steps：
1) selection mixes indica type japonica rice maintainer spring river with stigma exposing ratio height, Hua Shizao, anti-stripe virus disease characteristic
19B and with stigma exposing ratio is high, round-grained rice maintainer spring river 23B hybridizes in temperature sensitive type of Hua Shite morning characteristics, hybridization obtains F1Kind
Son；
2) by F1After adding generation, to F2Screened when the economical character of each individual plant of colony, stigma exposing ratio and flower, be selected in agriculture
Skill character is good, good stress resistance, stigma exposing ratio are high, Hua Shizao individual plant；Plant F3Strain, carry out economical character, resistance, post
Screening when head exposing ratio and flower, is selected in fine individual plant B36；
3) B36 carries out test cross with the japonica rice sterile spring river 16A with stigma exposing ratio height, Hua Shite morning characteristics；Plantation
Test cross F1, and to the test cross F with sterile holding capacity1It is returned with male parent B36；Start simultaneously at reference to combining ability test,
In Fertility identification, 1 year two generation, carry out transformation and obtain B6F1, neat and consistent in strain colony is returned, bagging self-fruitful rate is zero, agriculture
Skill character is consistent with stable fertility, cultivates compact plant type, dense cluster of short stem, Hua Shizao, outcrossing rate is good, hybrid seed yield is high, degeneration-resistant
Property strong middle round-grained rice sterile line spring river 35A, it is B36 that it, which is returned male parent,；
4) it is maternal with spring river 35A, is male parent with spring river 35B, breeds spring river 35A seed.
Xian round-grained rice osculant sterile line spring river 35A early when the present invention is a kind of colored, stigma exposing ratio is high propagation method, the party
Method comprises the following steps：Make female parent with spring river 35A, make male parent with B36, the spring river when early and middle ten dayses same period in June sowing, sowing
0.8-1.0 kgs/acre of 35A sowing quantities, B36 are 0.2-0.25 kgs/acre, and length of time rice seedlings grow control was at 20~30 days, father, maternal row ratio
2:8, maternal 20.0 centimetres of 16.7 cm x of seeding row spacing, it is single this or double originally plant；1-2 days before the cut phase, father, female parent prescind simultaneously
The sword-like leave of half, and spray 6-8 grams of " 920 " initial stage in two times in cut, the first Parent when earing inferior to 5% plant is all
4-6 grams/acre is sprayed, interval sprays second for 1-2 days, 2 grams/acre, catches up with powder to prescind maintainer in time after terminating.
The spring river 35A of the present invention has the advantages that：
1) it is best in quality：Analyzed according to Ministry of Agriculture's rice and quality of item supervision and inspection center, according to NY/T593-
2013《Edible rice varieties quality》Standard, in 12 indexs that sterile line spring river 35A maintainer spring river 35B is examined, head milled rice
Rate, transparency and amylose content reach primary standard, and Coarse Rice Rate and chalkiness degree reach secondary standard, grain length 6.2mm, more typically
Polished rice grain type is longer；
2) strong stress resistance；
3) outcrossing rate is high：Early when spring river 35A is spent, stigma exposure rate more than 50%, outcrossing is good, and system, breeding all compare
It is easier to, yield is higher.The hybridization group that spring in 2014, spring in 2015 and spring in 2016 configure in Hainan to spring river 35A
The production of hybrid seeds is closed, setting percentage is all more than 75%.If further research matched breeding high yield technique, it is expected to obtained more in larger area
High hybrid seed yield.
Brief description of the drawings
Fig. 1 is the breeding pedigree schematic diagram of the present invention.
Embodiment
The breeding method of the present invention is further illustrated below in conjunction with accompanying drawing (Fig. 1).
Sterile line spring river 35A breeding method：
A) selection mixes the indica type japonica rice maintainer spring with characteristics such as stigma exposing ratio height, Hua Shizao, anti-stripe virus diseases
River 19B and obtain F with round-grained rice maintainer spring river 23B in the temperature sensitive type of characteristic such as stigma exposing ratio is high, Hua Shite is early, hybridization1Kind
Son；
B) by F1After adding generation, to F2Screened when the economical character of each individual plant of colony, stigma exposing ratio and flower, be selected in agriculture
Skill character is good, good stress resistance, stigma exposing ratio are high, Hua Shizao individual plant.Plant F3Strain, carry out economical character, resistance, post
Screening when head exposing ratio and flower, is selected in fine individual plant.
C) by selected fine individual plant, continue with the late japonica rice sterile with characteristics such as stigma exposing ratio high, Hua Shite morning
Spring river 16A is that male parent is returned, and starts simultaneously at and carries out transformation acquisition with reference to combining ability test, Fertility identification, 1 year two generation
B6F1, neat and consistent in strain colony is returned, bagging self-fruitful rate is zero, and economical character is consistent with stable fertility, cultivates strain
Type is compact, dense cluster of short stem, Hua Shizao, outcrossing rate is good, hybrid seed yield is high, the middle round-grained rice sterile line spring river 35A of strong stress resistance.
Sterile line spring river 35A propagation method：
1st, preferable breeding field and suitable season are selected
Sterile line propagation field should select the field away from other rice, and to isolate naturally, preferable, wind is small, faces south, irrigation and drainage
Field convenient, soil property is good, preceding work is preferably non-rice.Preferably stagger the florescence with other rice seasonal, prevent string powder.
2nd, sowing time is determined
Single breeding evening, river 35A spring in In Hangzhou Region of Zhe Jiang Province, preferably sows spring river 35A and B36, under normal circumstances in early or mid June, the same period
Mid-September ears, and spring river 35A is bloomed in heading, it is early during flower and concentrate, the florescence it is short.
3rd, cultivate band tiller and strengthen seedling
0.8-1.0 kgs/acre of spring river 35A sowing quantity, spring river 35B are 0.2-0.25 kgs/acre, and rice seedling bed application rate rests in
7.0-8.0 kg/acre.The length of time rice seedlings grow is controlled at 25 days or so.
4th, rational planting proportion is determined
The rational planting proportion of Parent is：The planting proportion of female parent should be increased to greatest extent, increase having for female parent
Floret bears are imitated, ensure enough pollen amounts again, improve outcrossing seed-setting rate.Determined according to experiment for many years, father during transplanting, female parent
Than 2：8 is more satisfactory, and male parent presses 20.0 centimetres of 16.7 cm x of seeding row spacing, and list is originally or double sheets are inserted.The maternal cm x of seeding row spacing 16.7
20.0 centimetres, every clump is inserted 2,24 centimetres of Parent spacing.Field is put in time, to promote to take root, suitable to apply guarantor's fertilizers for potted flowers, mu applies urea 5
Kilogram, 5 kilograms of potassium chloride, coordinate colony, individual is healthy and strong, cultivates big fringe, improves effective floret bears.With reference to fertilising observation in time
, such as there is difference in Parent ear differentiation process, and to growing the faster suitably more nitrogen fertilizer applications of a side, the side for growing slower is fitted
When more potassium applications, the Parent florescence is in optimal state of meeting.
5th, artificial supplementary pollination, setting percentage is improved
1-2 days before the cut phase, father, the maternal sword-like leave for prescinding half simultaneously, make paternal pollen be easy to fly away, improve female
This pollination rate.Cut initial stage, spray in two times 6-8 grams/acre of " 920 ", Parent all sprays during 5% plant heading for the first time, 4-6
Gram/acre, the next day spray second, 2 grams/acre, father, maternal heading is concentrated, is neat, elongate fringe neck, concentrate flowering pollination, coordinate people
Work supple-mentary pollination, outcrossing seed-setting rate is improved, obtain higher breeding yield.
6th, field insect pest preventing and controlling
The preventing and treating of the pest and disease damages such as rice blast, banded sclerotial blight, brown paddy plant hopper, leaf roll snout moth's larva, pink rice borer, striped rice borer is the same as general japonica rice variety.
The preventing and treating of stripe disease, it is important to which rice seedling bed is turned in time before surviving the winter eliminates weeds, avoids wheatland as far as possible, and nursery period is strengthened
The preventing and treating of rice small brown rice planthopper, cut off the approach of viral transmission.
7th, impurity elimination standard and technical essential
Using original seed level Parent seed breeding, and accomplish that place is isolated in 500 meters, no rice varieties.Strictly go
Miscellaneous pure keeping, it is ensured that seed quality, to accomplish that whole breeding time all answers strict impurity elimination, before and after particularly earing, strictly remove hybrid strain.
First 1-2 days of spray " 920 " are the key periods of impurity elimination for the first time, to remove the non-sterile strain such as the maintainer being mixed into female parent as early as possible,
Powder is caught up with to prescind maintainer in time after terminating, in good time harvesting, to ensure the seed quality of sterile line.In each rings such as receipts, solarization, accumulatings
Section prevents mechanical admixture, it is ensured that seed purity.
Claims (2)
1. Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high selection, it is characterised in that
This method comprises the following steps：
1) selection with stigma exposing ratio height, Hua Shizao, anti-stripe virus disease characteristic mix indica type japonica rice maintainer spring river 19B and
With stigma exposing ratio is high, round-grained rice maintainer spring river 23B hybridizes in temperature sensitive type of Hua Shite morning characteristics, hybridization obtains F1Seed；
2) by F1After adding generation, to F2Screened when the economical character of each individual plant of colony, stigma exposing ratio and flower, be selected in agronomy
Shape is good, good stress resistance, stigma exposing ratio are high, Hua Shizao individual plant；Plant F3Strain, carry out economical character, resistance, outside column cap
Screening when dew rate and flower, is selected in fine individual plant B36；
3) B36 carries out test cross with the japonica rice sterile spring river 16A with stigma exposing ratio height, Hua Shite morning characteristics；Plant test cross
F1, and to the test cross F with sterile holding capacity1It is returned with male parent B36；Start simultaneously at and reflected with reference to combining ability test, fertility
Fixed, in 1 year two generation, carried out transformation and obtains B6F1, neat and consistent in strain colony is returned, bagging self-fruitful rate is zero, economical character
It is consistent with stable fertility, cultivate compact plant type, dense cluster of short stem, Hua Shizao, outcrossing rate is good, hybrid seed yield is high, strong stress resistance
Middle round-grained rice sterile line spring river 35A, it is B36 that it, which is returned male parent,；
4) it is maternal with spring river 35A, is male parent with spring river 35B, breeds spring river 35A seed.
2. Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high propagation method, it is characterised in that
This method comprises the following steps：Make female parent with spring river 35A, make male parent with B36, when the early and middle ten dayses same period in June sowing, sowing
0.8-1.0 kgs/acre of spring river 35A sowing quantity, B36 are 0.2-0.25 kgs/acre, and length of time rice seedlings grow control was at 20~30 days, father, maternal row
Than 2:8, maternal 20.0 centimetres of 16.7 cm x of seeding row spacing, it is single this or double originally plant；1-2 days before the cut phase, father, female parent are cut simultaneously
The sword-like leave of half is removed, and 6-8 grams of " 920 " are sprayed in two times initial stage in cut, first is inferior to Parent when 5% plant ears
4-6 grams/acre is all sprayed, interval sprays second for 1-2 days, 2 grams/acre, catches up with powder to prescind maintainer in time after terminating.
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| CN201710767781.0A CN107360966A (en) | 2017-08-31 | 2017-08-31 | Xian round-grained rice osculant sterile line spring river 35A early when a kind of colored, stigma exposing ratio is high selection and propagation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN109076954A (en) * | 2018-09-27 | 2018-12-25 | 江苏沿江地区农业科学研究所 | A method of promote BT type japonica rice sterile to bloom ahead of time |
| CN112586338A (en) * | 2020-12-04 | 2021-04-02 | 国家粳稻工程技术研究中心 | Breeding method of japonica rice sterile line in early flowering |
| CN113383702A (en) * | 2021-05-25 | 2021-09-14 | 高邮周邶墩农业发展有限公司 | Breeding method of hybrid rice between subspecies of japonica indica-free glutinous rice and subspecies of japonica indica-free glutinous rice in short growth period |
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| CN101595840A (en) * | 2009-07-01 | 2009-12-09 | 中国水稻研究所 | A kind of allusion quotation loses the selection of the wide affine male sterile line of type high stigma exposure rate japonica rice rice |
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| CN101595840A (en) * | 2009-07-01 | 2009-12-09 | 中国水稻研究所 | A kind of allusion quotation loses the selection of the wide affine male sterile line of type high stigma exposure rate japonica rice rice |
| CN103609430A (en) * | 2013-11-19 | 2014-03-05 | 浙江省农业科学院 | Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN109076954A (en) * | 2018-09-27 | 2018-12-25 | 江苏沿江地区农业科学研究所 | A method of promote BT type japonica rice sterile to bloom ahead of time |
| CN109076954B (en) * | 2018-09-27 | 2022-09-09 | 江苏沿江地区农业科学研究所 | Method for promoting earlier flowering of BT type japonica rice sterile line |
| CN112586338A (en) * | 2020-12-04 | 2021-04-02 | 国家粳稻工程技术研究中心 | Breeding method of japonica rice sterile line in early flowering |
| CN113383702A (en) * | 2021-05-25 | 2021-09-14 | 高邮周邶墩农业发展有限公司 | Breeding method of hybrid rice between subspecies of japonica indica-free glutinous rice and subspecies of japonica indica-free glutinous rice in short growth period |
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Application publication date: 20171121 |