CN101919353A - Breeding method of hybrid rice seed - Google Patents

Abstract

The invention belongs to the field of hybrid vigor utilization, in particular to a breeding method of a hybrid rice seed. Sterile line 94A is used for being hybridized with restoring line Nonghua 19 to breed three-line hybrid rice seed Jiuyou No.9. The hybrid rice seed has the advantages of high yield, stable yield, strong disease resistance and high biological yield.

Description

A kind of selection of hybrid rice seed

Technical field

The present invention relates to a kind of selection of hybrid rice seed, is the method for utilizing the new combination of three line method seed selection hybrid rice.

Background technology

China is paddy rice big country, and rice breeding technology maintains the leading position in the world always.But because the growth of Chinese population, the food problem that reply increases population remains the problem that China's agricultural needs most solution.Though it is hybrid rice seed production that the cost of three lines paddy seed is higher than two, because two be the production of hybrid seeds, have a big risk, be subjected to the uncontrollability of weather effect.Plant industry company in order to reduce the risk of production, produce hybrid rice seed, be still the maximum method of present kind industry company's employing with three line method.The shortening of the alternative time limit of kind at present reaches in order to enlarge with planting the needs that industry company competes abroad, and the three lines paddy crossbreed of seed selection safety is a most important task in present China rice breeding circle.

Summary of the invention:

The objective of the invention is to utilize the hybrid vigour theory, select high-combining ability, economical character is good, genotype is isozygotied, parents' affiliation is far away hybridization between selfed lines for use, select high yield, disease-resistant hybrid rice seed.

The invention provides a kind of method of the paddy rice cross breeding production of hybrid seeds, comprise the seed selection inbred line, safety is isolated, and regulates date of seeding, suitable row ratio, and roguing, results are utilized two-line sterile line 94A and are recovered the magnificent 19 assembly hybrid rice seeds of system's farming in good time.

Wherein said three is male sterile line 94A changes the crossbreed of B hybridization assembly with excellent IB/L301B//inland river phenanthrene, breeds through 8 generations of selfing.Applied for the protection of new variety of plant power.These male sterile line leaf sheath purple lines, dark green leaf is during leaf leaf second from the bottom is long, Ye Kuanzhong, the blade fine hair is dredged, tip of a leaf white, tip of a leaf length is long, tip of a leaf shape two is split, the pulvinus purple, sword-like leave blade warp, angle of glume opening is big, it is long to open the grain husk time, when flower scope long, the peak is early when colored, the water stain shape of flower pesticide, column cap white is long in the cane length, thickness is medium, angle osculant, the stem number is many, base portion stipes bag, joint look purple, internode look redness, sword-like leave is of moderate length, width is medium, angle is upright, and the stem number of blade is few, and the spike length degree is medium, fringe degree of stretching out is partly extracted out, fringe type osculant, and secondary branch stalk is few, in the cane resting bud vigor, grain husk shell fine hair is many, and clever pinnacle point is red, and the distribution of awns does not have, number of grain per ear is many, shaky, the seed holding height, sterile lemma is of moderate length, the sterile lemma redness, grain husk shell yellow is in the grain length, narrow, shape is elongated, in the thousand kernel weight, in the brown rice, spindle-like, it is light yellow to plant skin, and sterile plant rate is high, and the restorability of male sterile line is good.

The crossbreed of wherein said recovery system farming China 19 after with the extensive 838 hybridization assembly of (10-19//japonica rice 1394 is selected in samsara 422/) * spoke selects fine individual plant, and continuous 6 generations selfing is bred.

The biomaterial 94A that the present invention relates to, samsara 422, select 10-19, japonica rice 1394,838 all open in non-patent literature.

94A is bulletin in " the new variety of agricultural plant power protection communique " published on July 1st, 2009.

Wherein samsara 422, select 10-19, Gan Chaohong at Peng Yingcai; 9 excellent 3190[J in the new combination of precocious high blast resisting hybrid early]; Hybrid rice; 2006 06 phases; Open among the P80-81.

Open in the 9th page of its intermediate keng rice 1394 Jiangxi Province variety of crops validation boards the 6th meeting authorization kind catalogue authorization kind.

Wherein spoke extensive 838 is at Deng Wenmin, Deng Dasheng, and Wu Wanyi, Yang Chengming, Liu Yongqiang, poplar east feelings, it is the 02nd interim open that Chen Lihua, radiation research and radiation process journal were rolled up in 2005 the 23rd.

Advantage of the present invention and good effect are:

1, output height: Zhejiang in 2008 district test result: 518.5 kilograms of average yield per mus, comparison reaches significance level according to excellent No. 10 volume increase 8.7% of Shan; 2. best in quality: the outward appearance commodity grain of selling well well.

2, resistance: plant 2 years resistances of little 2007-2008 of institute through the province academy of agricultural sciences and identify, 0 grade of average leaf pest, 3.4 grades of fringe pest, fringe pest percent of loss 11.3%, composite index is respectively 1.1 and 3.9; 7.5 grades of bacterial leaf-blights, 9.0 grades of brown back rice plant hoppers.4. wide adaptability: be adapted at Henan, the semilate rice plantation of area, the middle and lower reach of Yangtze River.

3, rice is of fine quality, survey through Ministry of Agriculture's rice and 2 years Mi Zhijian of the quality of item supervision 2007-2008 of inspection center, average head rice rate 59.0%, aspect ratio 3.1, the white grain of chalk rate 44.5%, chalkiness degree 7.2%, 2 grades of transparencies, glue denseness 72.0mm, amylose content 20.6%, its 2 years rice matter indexs reach edible rice varieties quality 3 grades promulgated by the ministries or commissions of the Central Government and 3 etc. respectively.

Embodiment

Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.

Embodiment 1: the concrete seed selection process that crossbreed of the present invention nine is excellent No. 9

Nine excellent be for No. 9 to be that male sterile line 94A breeds with recovering 19 hybridization of system farming China by three, 94A changes the crossbreed of B hybridization assembly with excellent IB/L301B//inland river phenanthrene, breeds through 8 generations of selfing.Recover the crossbreed of system farming China 19 after with the extensive 838 hybridization assembly of (10-19//japonica rice 1394 is selected in samsara 422/) * spoke, select fine individual plant, continuous 6 generations selfing is bred.

Embodiment 2: the production technology that crossbreed of the present invention nine is excellent No. 9

(1) safety is isolated: space length＞500 meter, or other rice varieties of heading flowering period of being separated by more than 15 days carries out the production of hybrid seeds and isolates.

(2) begin sowing in good time: first phase male parent is than maternal Zao inserting 25～30 days, or leaf order difference 6.5-7.5 leaf, or the effective accumulated temperature father and mother originally differ from, and (area is maternal in the Soviet Union sows 20-25 day in May about 205 ℃, sowing about the April 24 male parent first phase), the second phase, the third phase are separated by about 7 days with preceding first phase respectively, or are separated by 12 days with two phase male parents.

(3) rational close planting, suitable row ratio: 16.7 * 10 centimetres of seeding row spacing female parents, plant foot 15～180,000 basic seedlings for every mu, the male parent spacing in the rows is 16.7 centimetres, and the aisle was 33.3 centimetres when duplicate rows was planted, and father and mother's one's own profession ratio is 2: 20 or 1: 12.Row is to vertical with locality predominant wind at heading stage.

(4) use " 920 " technology and artificial supplementary pollination; Spray 920 during 10% female parent heading, every a spray 20 grams, the 4th spray 5 grams.Catch up with pollen to carry out artificial supplementary pollination whole flowering stage every day at florescence from fringe phase beginning to neat fringe.

(5) prevent and treat the kernel smut technology: black spirit (producer's medicine) prevents and treats kernel smut 2-3 time continuously at cut phase, fringe phase beginning and full heading time with going out.

(6) roguing technology: whole growing is pulled out the high temperature sensitive variant of removing the loose powder phenomenon among this outside proterties such as variant of form, fertility and father and mother and the maternal 94A, contains spend back about 23 days timely results hybrid seed.

Embodiment 3: crossbreed nine excellent No. 9 cultivation application technologies

(1) begins sowing in good time: at the sowing on May 8 of area, the middle and lower reach of Yangtze River, length of time rice seedlings grow 30-40 days, the rice seedling bed amount of broadcasting 1.0-1.5 kilogram/mu.

(2) transplant density and colony's requirement: make in the season rice culture and generally be advisable with 26.7 * 13.3 centimetres, basic seedling 8.5-10 ten thousand, plant back 15 days stem tiller numbers and reach predetermined spike number, plant and reached peak seedling in back 30 days and be no more than 270,000, become fringe 18-20 ten thousand at last, every mu of total grain husk flower 3600-4000 ten thousand, ripening rate is more than 85%, more than thousand kernel weight 25 grams.

(3) fertilizer application: always use 15 kilograms of purity nitrogens, basal plane is fertile in principle wants sufficient, and early stage, fertilizing amount accounted for always with nitrogen amount about 75%.

(4) water starch management: shallow irrigation after the rice transplanting, after stem tiller number reaches predetermined spike number, gently put several times and at last the field is baked in fact.Pustulation period is based on water, alternation of wetting and drying.Harvesting can be dewatered the last week.

(5) crossbreed can be used as rice culture in the basin, the middle and lower reach of Yangtze River, also can be used as South China two morning in seasons of late rice.

(6) attention control rice blast and smut.

Claims (7)

1. the selection of a hybrid rice seed comprises that the sowing father and mother originally, spray gibberellin maternal plant heading stage, it is characterized in that: with three is that male sterile line 94A is the magnificent 19 group gas-mixing hybridization seed selection ternary hybrid rices of farming with recovering.Maternal 94A is that the high yield and high quality three by Beijing Jinse nonghua seed industry Technology Co., Ltd's seed selection is a male sterile line; It is new restorer lines that (10-19//1394 are selected in samsara 422/) * 838 pedigree seed selection forms that male parent is recovered system farming China 19.

2. method according to claim 1 is characterized in that safe isolation distance＞500 meter, or is separated by with this heading flowering period of described father and mother and carries out the production of hybrid seeds more than 15 days and isolate.

3. method according to claim 1, it is characterized in that first phase male parent than maternal Zao inserting 25～30 days, or leaf order difference 6.5-7.0 leaf, or the effective accumulated temperature father and mother originally differ from 200 ℃, the second phase, the third phase were separated by 7 days with preceding first phase respectively, or were separated by 10 days with two phase male parents.

4. method according to claim 1 is characterized in that 16.7 * 10 centimetres of seeding row spacing female parents, and the male parent spacing in the rows is 16.7 centimetres, and the aisle was 33.3 centimetres when duplicate rows was planted, and father and mother's one's own profession ratio is 2: 20 or 1: 12.

5. method according to claim 1 is characterized in that 10% of maternal total spike number sprays gibberellin 920 when earing, every a spray 20 grams, spray in the 4th day 5 grams.

6. method according to claim 1 and 2 is characterized in that preventing and treating kernel smut 2-3 time continuously at cut phase, fringe phase beginning and full heading time.

7. method according to claim 1 is characterized in that whole growing pulls out outside proterties and the discrepant plant of maternal 94A, contains and spends back 25 days results hybrid seeds.