CN103430823A - Two-line hybrid rice seed breeding method - Google Patents
Two-line hybrid rice seed breeding method Download PDFInfo
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Abstract
The present invention discloses a two-line hybrid rice seed Yanliangyou 1668 breeding method, and belongs to the field of rice hybrid seed production. According to the present invention, an indica type photo-thermosensitive genic male sterile line Yan 166S is adopted as a female parent, and an indica type two-line restorer line Yanhui 888 is adopted as a male parent to produce the two-line hybrid rice seed Yanliangyou 1668, wherein the preservation number of the Yan 166S is CGMCCNO.7701; and the hybrid rice seed has characteristics of moderate mature stage, high yield, stable yield, national standard grade II good quality rice, and strong disease resistance.
Description
Technical field
The present invention relates to paddy rice cross breeding seed production field, specifically a kind of breeding method of double-linear hybrid rice seeds salt two excellent 1668.
Background technology
Paddy rice is the staple food crop of China, and the cultivated area of hybrid rice accounts for the over half of China's Monitoring of Paddy Rice Plant Area.The improvement of double-linear hybrid rice kind is the major fields of China's agri-scientific research in the recent period, and high-quality, high yield, many anti-, wide fitting are the important long-term targets of rice breeding.It is one of important channel of realizing China's breeding of hybridized rice third phase target that the ideotype that Academician YUAN Long-ping proposes and hybrid vigour combine.
Nearly ten years, China's breeding of hybridized rice research has obtained the achievement that makes the world attract attention, in succession being bred as the super double-linear hybrid rices such as " two line system ", " Liangyou0293 ", " excellent No. 2 of Y two " newly combines, and realizing China's super rice target of hundred mu of first, second and third phases of high yield side's per mu yield, sign China's rice breeding and production continue to keep world lead level.China's super hybrid rice realizes that the breeding objective of first, second and third phase is also the yield level in hundred mu of sides and thousand mu of high yield sides, and the yield level that China's paddy rice large tracts of land is produced has just realized that per mu yield exceedes 500 kilograms, average yield per mu does not also reach 550 kilograms of levels.At present, super double-linear hybrid rice new varieties ubiquity high yield and inharmonic defects such as resistance, high yield and high-quality of application in production.Seed selection, production and popularization rice quality reach national fine quality rice standard, stable high yield, the ripe phase is moderate, disease resistance is strong new hybrid rice varieties is the effective way that further improves China's hybrid rice production output, ensures national food safety.
Summary of the invention
The object of the invention is to for ensureing that China's grain-production reaches safely the double-linear hybrid rice that China's Rice Production market safety cultivates and newly combines, and a kind of breeding method that obtains the double-linear hybrid rice seeds of high yield is provided.
A kind of breeding method of double-linear hybrid rice seeds salt two excellent 1668, select indica type photo-thermo-sensitive genic male sterile line " salt 166S " for maternal, and take indica type two is that restorer " salt extensive 888 " be male parent, production double-linear hybrid rice seeds " salt two excellent 1668 ".Described salt 166S has been deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 29th, 2013, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address, deposit number: CGMCC NO.7701, Classification And Nomenclature paddy rice (Oryza sativa).Salt extensive 888 is bred as for Jiangsu Inst. of Coastal Area Agricultural Sciences, just, in large tracts of land production of hybrid seeds application, can buy by market approach.
Described method, the space length >=500m of safety isolation, or time isolation, around 500m with the heading flowering period of the rice varieties of interior plantation, with the heading flowering period of described father, female parent, be separated by more than 15d.
Described method, male parent is divided two phases sowings, the more maternal early sowing 36～38d of the 1st phase male parent, or leaf age differs from 6.6～6.8 leaves, two male parent dates of seeding phase, differed 10～12d.
Described method, production of hybrid seeds old farmer, maternal row are planted with period interval than 2: 12～14, two phase male parent duplicate rows, male parent distance between rows and hills 30cm * 15cm; Maternal distance between rows and hills 16.7cm * 13.3cm, 2～3, every cave, plant sufficient Basic Seedling.
Described method, the total consumption 35～40g/ mu of seed farm " 920 ", divide and spray for 3 times, cuts leaf for the first time when female parent heading 5-10% and sprayed 15g/ mu the same day, cuts the leaf second day and spray 15～20g/ mu, cuts leaf and within the 4th day, spray 5～10g/ mu.
Described method, be shown in that fringe phase, fringe phase beginning, full heading time prevent and treat rice kernel smut 3～4 times continuously.
Described method, the paddy rice production of hybrid seeds will be prevented assorted pure keeping the time of infertility, emphasis is the strict impurity elimination of heading flowering period 3～4 times, pulls out and father, maternal discrepant plant, contains bow structure bundle 25～30d, first by the clean rear results hybrid seed of male parent harvesting.
The present invention utilizes indica type photo-thermo-sensitive genic male sterile line " salt 166S " for maternal, take indica type two be restorer " salt extensive 888 " for two of male parent production be that the beneficial effect of hybrid rice seed " salt two excellent 1668 " is as follows:
Double-linear hybrid rice " the salt two excellent 1668 " stable high yield that the present invention produces: within 2009, participate in medium rice district, Jiangsu Province multiple spot variety comparative test, average yield 598.6kg, than excellent 084 volume increase 5.69% of contrast II.Within 2010, participate in medium rice district, Jiangsu Province multiple spot variety comparative test, average yield 566.8kg, than excellent 084 volume increase 3.94% of contrast II, reach utmost point significance level.Within 2011, participate in medium rice district, Jiangsu Province multiple spot variety comparative test, average yield 587.5kg, than excellent 084 volume increase 5.72% of contrast II, reach utmost point significance level.Within 2013, participate in medium rice district, Jiangsu Province multiple spot great Qu demonstration test.
Double-linear hybrid rice " the salt two excellent 1668 " rice quality that the present invention produces is excellent: 2009 according to Ministry of Agriculture's rice and quality of item supervision and inspection center test (Wuhan: Hua Zhong Agriculture University): brown rice rate 80.8%, polished rice rate 72.3%, head rice rate 67.2%, grain length 7.1mm, aspect ratio 3.1, the white rate 20% of chalk, chalkiness degree 1.8%, 2 grades of transparencies, disappear 6.8 grades of values of alkali, glue denseness 68mm, amylose content 16.4%, the all indexs of rice quality all reach GB secondary superior rice standard, good palatability.
Double-linear hybrid rice " the salt two excellent 1668 " disease resistance that the present invention produces is strong: 2009 according to Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province inoculated identification result: bacterial leaf spot resistant JS49-6 pathogenic strain (1 grade), bacterial leaf spot resistant KS-6-6 pathogenic strain (1 grade), middle bacterial leaf spot resistant Zhejiang 173 pathogenic strains (3 grades), in anti-PX079 pathogenic strain (3 grades), to 2 biological strains such as rice blast ZB10, ZF1, show disease-resistantly, sick level is 1 grade; To all immunity of 4 biological strains such as rice blast ZC15, ZD7, ZE3, ZG1, sick level is 0 grade, anti-panicle blast (1 grade), and middle sense banded sclerotial blight (MS), anti-stripe virus disease (R), lodging resistance is stronger.
Double-linear hybrid rice " the salt two excellent 1668 " economical character that the present invention produces is good: " salt two excellent 1668 " seedling is short strong, the short and thick green of bud scale, and blade is straight and upright, and the leaf look dark green, and stooling is stronger.The strain plant type is collecting and distributing moderate, plant height 125cm left and right, and the total blade 17-18 of stem opens, 5 extend internodes, three, top functional leaf is straight and upright, is subject to the light attitude good, lifts on sword-like leave when ripe and is the straw mulch top, the blue or green seed yellow maturity lover of bar.The percentage of earbearing tiller 65% left and right, general mu is fringe 15-17 ten thousand effectively, and the fringe type is larger, spike length 25.5cm, several 185 left and right of every total grain panicle, several 150 left and right of the real grain of every fringe, ripening rate 85% left and right, more than thousand kernel weight 29g, slightly short awns.The time of infertility, about 150d, than the slow ripe 3-5d of contrast II excellent 084, belonged to the basic nutrition vegetative form, in ripe indica hybrid rice type.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1: the production technology of hybrid rice of the present invention " salt two excellent 1668 " seed
Select indica type photo-thermo-sensitive genic male sterile line " salt 166S " for maternal, take indica type two is that restorer " salt extensive 888 " be male parent, production double-linear hybrid rice seeds " salt two excellent 1668 ".Described salt 166S has been deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 29th, 2013, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address, deposit number: CGMCC NO.7701, Classification And Nomenclature paddy rice (Oryza sativa).Salt extensive 888 is bred as for Jiangsu Inst. of Coastal Area Agricultural Sciences, just, in large tracts of land production of hybrid seeds application, can buy by market approach.
(1) safety isolation: space length >=500m, or around time isolation, 500m is separated by more than 15d with the heading flowering period of described father, female parent with the heading flowering period of the rice varieties of interior plantation.
(2) begin sowing in good time: the two phases sowing of extensive 888 minutes of male parent salt, the more maternal early sowing 36～38d of the 1st phase male parent, or leaf age differs from 6.6～6.8 leaves, two male parent dates of seeding phase, differed 10～12d.
(3) rational close planting, suitable row ratio.Production of hybrid seeds old farmer, maternal row are planted with period interval than 2: 12～14, two phase male parent duplicate rows, male parent distance between rows and hills 30cm * 15cm; Maternal distance between rows and hills 16.7cm * 13.3cm, 2～3, every cave, plant sufficient Basic Seedling.
(4) use " 920 " technology and artificial supplementary pollination.The total consumption 35～40g/ mu of seed farm " 920 ", divide and spray for 3 times, cuts leaf for the first time when female parent heading 5-10% and sprayed 15g/ mu the same day, cuts the leaf second day and spray 15～20g/ mu, cuts leaf and within the 4th day, spray 5～10g/ mu.From fringe phase beginning to neat fringe, catch up with pollen to carry out artificial supplementary pollination 5-6 time whole flowering stage every day at florescence.
(5) prevent and treat the kernel smut technology: cut phase, fringe phase beginning and for full heading time bactericide 17.5% wetting powder (production of chemical plant, the Changjiang river, Taicang City, Jiangsu Province) that " goes out black No. 1 " prevent and treat continuously kernel smut 3～4 times.
(6) pure technology is gone in impurity elimination: the paddy rice production of hybrid seeds will be prevented assorted pure keeping the whole time of infertility from the rice seedling bed to the land for growing field crops, and emphasis is the strict impurity elimination of heading flowering period 3～4 times, pulls out form and the discrepant plant of maternal " salt 166S " and male parent " salt extensive 88 ".Contain bow structure bundle 25～30d, first by the clean rear results hybrid seed of male parent harvesting.
Embodiment 2: the concrete Breeding Process of hybrid rice seed of the present invention " salt two excellent 1668 ".
The hybrid rice seeds that the present invention produces " salt two excellent 1668 ", in the New Hybrid Rice Combination variety comparative test of 2009～2011 years participation our unit tissues, shows stable high yield, and average yield per mu is increased production 5% left and right than contrast II excellent 084.In Table 1.
Table 1:2009-2011 salt two excellent 1668 variety comparative test output situations
The hybrid rice seeds that the present invention produces " salt two excellent 1668 ", within 2009, according to the test of Ministry of Agriculture's rice and quality of item supervision and inspection center, (Wuhan: Hua Zhong Agriculture University), all indexs of its rice quality all reach GB superior rice standard more than three grades.In Table 2.
Table 2:2011 salt two excellent 1668 rice quality indexs
The hybrid rice seeds that the present invention produces " salt two excellent 1668 ", 2009 according to Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province inoculated identification result: bacterial leaf spot resistant JS49-6 pathogenic strain (1 grade), bacterial leaf spot resistant KS-6-6 pathogenic strain (1 grade), middle bacterial leaf spot resistant Zhejiang 173 pathogenic strains (3 grades), in anti-PX079 pathogenic strain (3 grades), to 2 biological strains such as rice blast ZB10, ZF1, show disease-resistantly, sick level is 1 grade; To all immunity of 4 biological strains such as rice blast ZC15, ZD7, ZE3, ZG1, sick level is 0 grade, anti-panicle blast (1 grade), and middle sense banded sclerotial blight (MS), anti-stripe virus disease (R), lodging resistance is stronger.
Table 3:2009 salt two excellent 1668 Disease Resistance Identification results
Embodiment 3: hybrid rice of the present invention " salt two excellent 1668 " high-yield culture technique
(1) proper seeding, cultivate strong seedling: according to various places Rice Production custom, reasonable arrangement sowing time, guarantee safety heading flowering.10～15 kilograms of every mu of clean seedling plate amounts of broadcasting of water-raised seedling, 20～25 kilograms of every mu of clean seedling plate amounts of broadcasting of non-irrigated seedling, 1.0～1.5 kg/acres of land for growing field crops sowing quantities.1.5～2.0 kilograms of every mu of land for growing field crops sowing quantities of transplanting.Rice seedling bed will use sufficient base manure, early executes wean fertile, imposes long thick short fetilizer for tillering, send and transfer fertilizer, and carry out rice seedling bed disease pest and weed control, cultivates the strong seedling of of the right age many tillers.
(2) transplant rational close planting: transplant, rational close planting is the basis of high-yield culturing in good time in good time.At 30～35 days water-raised seedling length of time rice seedlings grow, the non-irrigated seedling length of time rice seedlings grow was advisable with 20～25 days, general 18～20 days of the transplanting length of time rice seedlings grow.In upper fertility field plant soon specification 1.6～1.8 ten thousand caves/mu left and right, 1～2, every cave, plant sufficient Basic Seedling 6～80,000/mu, it is 1.8～2.0 ten thousand caves/mu that the field that fertility is low will suitably increase Planting Densities, accomplishes shallow slotting, even cultivation while planting, and guarantees to plant quality.
(3) scientific utilization of fertilizer, short seedling is early sent out: scientific utilization of fertilizer, it is the key that forms yielding Populations that short seedling is early sent out.Per mu yield is cultivated more than 650 kilograms, and general fertilizer requirement amounts to purity nitrogen 20 kg/acres of left and right, and phosphorus, potash fertilizer are used in collocation, phosphorus, potash fertilizer are main as base manure, and fertilising should be adhered to " front short, in steady, rear benefit ", principle, base manure accounts for 60% left and right, and tillering fertilizer accounts for 15% left and right, and fringe granulated fertilizer accounts for 25% left and right.
(4) run water slurry well, coordinate colony: water starch management should take that shallow water transplants seedlings, very little water is lived, thin moisture tiller, deep water heading flowering, the irrigation method of later stage alternation of wetting and drying.Require to plant latter about 20 days, send out foot and wait the fringe seedling, field is put in draining in good time, controls peak seedling and is no more than 250,000/mu, finally becomes fringe 16～170,000/mu, and moistening endowment of later stage, gather in and cut off the water supply in first 5 days, guarantees the blue or green seed Huang of stalk.
(5) the anti-worm of curing the disease, keep a full stand of seedings and increase fringe: broadcast front with medicament seed soaking and prevent and treat the seed-borne diseases such as bakanae disease and Aplelenchiodes bessegi of rice.Seeding stage attention control small brown rice planthopper, rice thrips, aphid, field period will be carried out the preventing and controlling of the damage by disease and insect such as control rice blast, bacterial leaf-blight, banded sclerotial blight, black streak dwarf, snout moth's larva, brown plant-hopper, guarantees that the rice seedling normal growth grows, and keeps a full stand of seedings to strive fringe and strive for high yield.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (7)
1. the breeding method of a double-linear hybrid rice seeds salt two excellent 1668, it is characterized in that, select indica type photo-thermo-sensitive genic male sterile line " salt 166S " for maternal, take indica type two is that restorer " salt extensive 888 " is male parent, produce double-linear hybrid rice seeds " salt two excellent 1668 ", the deposit number of described salt 166S: CGMCC NO.7701.
2. method according to claim 1, is characterized in that, the space length >=500m of safety isolation, or time isolation, around 500m with the heading flowering period of the rice varieties of interior plantation, with the heading flowering period of described father, female parent, be separated by more than 15d.
3. method according to claim 1, is characterized in that, male parent is divided two phases sowings, the more maternal early sowing 36～38d of the 1st phase male parent, or leaf age differs from 6.6～6.8 leaves, and two male parent dates of seeding phase, differed 10～12d.
4. method according to claim 1, is characterized in that, production of hybrid seeds old farmer, maternal row are planted with period interval than 2: 12～14, two phase male parent duplicate rows, male parent distance between rows and hills 30cm * 15cm; Maternal distance between rows and hills 16.7cm * 13.3cm, 2～3, every cave, plant sufficient Basic Seedling.
5. method according to claim 1, is characterized in that, the total consumption 35～40g/ mu of seed farm " 920 ", divide and spray for 3 times, cut leaf for the first time when female parent heading 5-10% and sprayed 15g/ mu the same day, cut the leaf second day and spray 15～20g/ mu, cut leaf and within the 4th day, spray 5～10g/ mu.
6. method according to claim 1, is characterized in that, seeing that fringe phase, fringe phase beginning, full heading time prevent and treat rice kernel smut 3～4 times continuously.
7. method according to claim 1, is characterized in that, the paddy rice production of hybrid seeds will be prevented assorted pure keeping the time of infertility, emphasis is the strict impurity elimination of heading flowering period 3～4 times, pull out and father, maternal discrepant plant, contain bow structure bundle 25～30d, first by the clean rear results hybrid seed of male parent harvesting.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585025A (en) * | 2015-02-03 | 2015-05-06 | 盐城明天种业科技有限公司 | Method for breeding broad-spectrum rice restorer line hybrid rice combination |
| CN105660386A (en) * | 2016-03-29 | 2016-06-15 | 浙江省嘉兴市农业科学研究院（所） | Breeding method of conventional early Indica rice |
| CN106332771A (en) * | 2016-08-08 | 2017-01-18 | 盘锦北方农业技术开发有限公司 | Method for cultivation of high-yield, high-quality and multi-resistant paddy rice new variety |
| CN114982631A (en) * | 2022-05-25 | 2022-09-02 | 江苏省徐州大华种业有限公司 | Two-line hybrid rice breeding method suitable for machine insertion |
-
2013
- 2013-06-19 CN CN2013102420313A patent/CN103430823A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585025A (en) * | 2015-02-03 | 2015-05-06 | 盐城明天种业科技有限公司 | Method for breeding broad-spectrum rice restorer line hybrid rice combination |
| CN104585025B (en) * | 2015-02-03 | 2017-04-19 | 盐城明天种业科技有限公司 | Method for breeding broad-spectrum rice restorer line hybrid rice combination |
| CN105660386A (en) * | 2016-03-29 | 2016-06-15 | 浙江省嘉兴市农业科学研究院（所） | Breeding method of conventional early Indica rice |
| CN106332771A (en) * | 2016-08-08 | 2017-01-18 | 盘锦北方农业技术开发有限公司 | Method for cultivation of high-yield, high-quality and multi-resistant paddy rice new variety |
| CN114982631A (en) * | 2022-05-25 | 2022-09-02 | 江苏省徐州大华种业有限公司 | Two-line hybrid rice breeding method suitable for machine insertion |
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Application publication date: 20131211 |