CN101663993A - Culture method of round grain glutinous rice variety resisting stripe virus disease - Google Patents

Abstract

The invention relates to a culture method of a round grain glutinous rice variety resisting a stripe virus disease, belonging to the technical field of plant breeding. The culture method comprises thefollowing steps: using (Zhendao No.2*Yanjing No.2) F1 formed by hybridizing Zhendao No.2 (sticky rice) and Yanjing No.2 as a female parent and (Sidao No.8*Sidao No.9) formed by hybridizing Sidao No.8and Sidao No.9 as a male parent for sexual hybridization; selecting the yield, the resistance and the sticky quality of continuous nine generations added in a normal season in Yancheng, Jiangsu and in Sanya, Hainan to culture a new round grain glutinous rice variety Yangjingnuo No.2 with high rice quality reaching an international excellent-quality sticky rice level, high yield level, stripe virus disease resistance, suitable maturity period, wide suitability and excellent comprehensive properties.

Description

A kind of breeding method of resisting stripe virus disease glutinous rice variety

One, technical field

The breeding method of a kind of resisting stripe virus disease glutinous rice variety of the present invention belongs to field of plant variety breeding technology, more specifically says so to be exclusively used in seed selection resisting stripe virus disease glutinous rice new varieties.

Two, background technology

China has rice breeding advanced in the world achievement, has successively successfully implemented the rice dwarf breeding, three is, the double-linear hybrid rice breeding.Paddy rice is the first crops of China, the improvement of rice varieties is the major fields of China's agri-scientific research always, high-quality, high yield, resist are the important long-term targets of rice breeding more, Jiangsu is the big province of japonica rice, the japonica rice breeding in Jiangsu Province has obtained great success during " eight or five ", bred large quantities of high yields, high-quality, many anti-japonica rice new varieties, made positive contribution for ensureing China's grain security.The extraordinary rice breeding in Jiangsu Province especially round-grained rice glutinous rice breeding of new variety makes fast progress, and has bred a collection of round-grained rice glutinous rice new varieties.Along with the enriching constantly and improve of living standards of the people, large tracts of land production presses for resistance, glutinous property is good, output is high round-grained rice glutinous rice new varieties.

Stripe disease is a kind of important disease of Jiangsu and contiguous province Rice Production in recent years, caused great harm to Jiangsu and contiguous province Rice Production, present Jiangsu Province and each rice district, contiguous province be the late ripe middle glutinous kind of round-grained rice of nonreactive stripe virus disease large tracts of land popularizing planting on producing still.If will resist stripe disease characteristic and high yield and high-quality to combine, extensive adaptability and the stable high yield and the high-quality performance of high-quality round-grained rice glutinous rice new varieties will be strengthened further.

The jiangsu coast area institute of agricultural sciences and Yancheng kind tomorrow industry Science and Technology Ltd. use " breeding method of resisting stripe virus disease glutinous rice variety " common seed selection in the recent period yield level higher, rice matter reaches GB quality glutinous rice standard, resistance especially shows glutinous No. 2 of the round-grained rice glutinous rice variety salt round-grained rice of anti-stripe virus disease, 2006-2008 participates in Jiangsu Inst. of Coastal Area Agricultural Sciences's japonica rice strain and identifies, the strain variety comparative test, the performance comparison significantly or is extremely significantly increased production according to kind, rice matter reaches GB quality glutinous rice standard, high anti-stripe virus disease (HR), the ripe phase is moderate, wide adaptability, characteristics such as comprehensive proterties is good have good prospects for application.

Three, summary of the invention

Technical problem

The objective of the invention is to solve above-mentioned problems of the prior art with not enough, a kind of breeding method of resisting stripe virus disease glutinous rice variety is provided.

Technical scheme

The breeding method of a kind of anti-stripe virus disease round-grained rice glutinous rice variety of the present invention, its technical scheme is as follows:

A) select with pressing down (No. 2, No. 2 * salt of town rice round-grained rice) F that rice forms with No. 2 hybridization of salt round-grained rice for No. 2 ₁Be female parent, (No. 8 * nasal mucus of the nasal mucus rice rice No. 9) F that forms with No. 9 hybridization of nasal mucus rice No. 8 and nasal mucus rice ₁Be male parent, sexual hybridization obtains F ₀Seed;

B) in 9 generations of selfing then,, selfing limit, limit is selected: select anti--middle bacterial blight-resisting, sick level 0-3 level; Anti--middle blast resisting, sick level 0-3 level; Anti-stripe virus disease is disease-resistant, sick level 0-2 level, incidence of disease 0-15%; The plant type compactness, blade is short very, falls the length 25-30cm of 1,2,3 leaves, thousand kernel weight 25-26 gram, the white rate of chalk≤30%, chalkiness degree≤5%, the strong fine individual plant of glutinous property;

From selfing F ₂Generation beginning, take the field to identify by generation and require: the plant type compactness, blade is very short, falls the length 25-30cm of 1,2,3 leaves, thousand kernel weight 25-26 gram; Identify exterior quality, the white rate of leading indicator chalk≤30%, chalkiness degree≤5% before the paddy maturation; Indoorly carrying out artificial threshing again, going out its glutinous property of rough evaluation again after the results, keeping the fine individual plant (fringe) that meets breeding objective and enter the seed selection work that next breeding generation repeats the previous generation again;

From selfing F ₃Generation beginning takes the capable 33cm+16.5cm of width, spacing in the rows 16.5cm, high fertile mu to execute the planting type that pure N17 kilogram, thin planting mu are planted 1.5 ten thousand caves, carries out artificial infection by generation and identifies bacterial leaf spot resistance, sick level index 0-3 level; Identify its rice blast resistance under field conditions (factors), sick level index 0-3 level; Carry out the stripe virus disease resistance under field conditions (factors) and identify, sick level index 0-2 level, incidence of disease index 0-15%;

9 generations of selfing are F ₉Glutinous No. 2 of resisting stripe virus disease glutinous rice variety salt round-grained rice have been bred, this kind 150-152 in the time of infertility days, plant height 90-95cm, blade is very short, promptly falls 1,2, the length of 3 leaves is 25-30cm, and the leaf look moderate, blade is straight and upright, and stem total leaf number 17-18 opens, 5 elongation internodes, sowing is to earing 105-108 days, and tillering ability is stronger, percentage of earbearing tiller 65-70%, general mu is fringe 20-22 ten thousand effectively, and spike length 18-21 centimetre, every total grain panicle 135-140 grain, the real grain of every fringe 125-130 grain, ripening rate 90-92%, thousand kernel weight 25-26 gram, no awns, all indexs of rice quality all reach GB quality glutinous rice standard, brown rice rate 83.3%, polished rice rate 71.7%, head rice rate 66.8%, grain length 5.0mm, disappear 6.3 grades of values of aspect ratio 1.8, alkali, glue denseness 100mm, amylose content 1.4%, glutinous property is strong, good palatability; Anti--middle bacterial blight-resisting, sick level 0-3 level; Anti--middle blast resisting resistance, sick level 0-3 level; High resisting-anti-stripe virus disease, sick level index 0-2 level, incidence of disease index 0-15%.

Beneficial effect

The resisting stripe virus disease glutinous rice new varieties salt round-grained rice that the present invention cultivates has following beneficial effect glutinous No. 2:

(1) rice is of fine quality, and glutinous property is strong

2008, through Ministry of Agriculture's rice and the test of quality of item supervision and inspection center, glutinous No. 2 all indexs of rice quality of salt round-grained rice all reach GB quality glutinous rice standard, good palatability, its result is: brown rice rate 83.3%, polished rice rate 71.7%, head rice rate 66.8%, grain length 5.0mm, aspect ratio 1.8, disappear 6.3 grades of values of alkali, glue denseness 100mm, amylose content 1.4%.

(2) high anti-stripe virus disease, hold concurrently bacterial blight-resisting and rice blast

2007-2008, the salt round-grained rice is identified the resistance result according to Institute of Plant Protection, academy of agricultural sciences, Jiangsu Province inoculation glutinous No. 2: bacterial leaf spot JS49-6 pathogenic strain (3 grades), bacterial leaf spot KS-6-6 pathogenic strain (1-3 level), bacterial leaf spot Zhejiang 173 pathogenic strains (1-3 level), PX079 pathogenic strain (1-5 level), to 7 all immunity of biological strain such as rice blast ZA5, ZB13, ZC5, ZD5, ZE3, ZF1, Z grams 1, sick level is 0 grade, the panicle blast resistance is (a 1-3 level), sharp eyespot resistance is (S), the stripe virus disease resistance is (HR), and contrast force is educated No. 3 stripe virus disease resistances of round-grained rice and is (HS).Sturdy because of glutinous No. 2 canes of salt round-grained rice simultaneously, leaf sheath is embraced the stem consolidation, has fertilizer resistance anti-lodging performance preferably.

(3) stable high yield, yield potential is big

The salt round-grained rice was participated in round-grained rice strain evaluation in this institute in glutinous No. 2 2006,620.1 kilograms of average yield per mus, and comparison is educated No. 3 volume increase 11.1% of round-grained rice according to force, reaches utmost point significance level; The round-grained rice strain is identified in these institutes of continuation participation in 2007,636.5 kilograms of average yield per mus, and comparison increases production 3.88% No. 8 according to raising the spoke round-grained rice, compares according to force and educates No. 3 volume increase 20.6% of round-grained rice, all reaches utmost point significance level; Participated in round-grained rice strain variety comparative test in this institute in 2008,641.6 kilograms of average yield per mus, comparison is according to raising No. 8 volume increase 4.6% of spoke round-grained rice, and comparison is educated No. 3 volume increase 20.6% of round-grained rice according to force, all reaches utmost point significance level.

(4) economical character is good, wide adaptability

Glutinous No. 2 rice cultures in Jiangsu Province does of salt round-grained rice, in the 150-152 days time of infertility, comparison was educated No. 3 slow ripe 1-2 of round-grained rice days according to force.The seedling body is strong and vigorous, the bud scale green, and the leaf sheath light green, blade is tall and straight, plant type is collecting and distributing moderate, plant height 90-95cm, stem number of blade 17-18 leaf, 5 elongation internodes, sowing is to earing 105-108 days, and cane is sturdy, the leaf sheath plumpness is embraced the stem consolidation, does not reveal joint, upper blade is tall and straight, and the length of 1,2,3 leaves is 25-30cm, is subjected to the light attitude good, functional period is long, and it is delicate and pretty to grow, and self regulating power is strong.Tillering ability is stronger, percentage of earbearing tiller 65-70%, and general mu is fringe 20-22 ten thousand effectively, and spike length 18-21 centimetre, every total grain panicle 135-140 grain, the real grain of every fringe 125-130 grain, ripening rate 90-92%, thousand kernel weight 25-26 gram, no awns, ripe lover when ripe.

Four, description of drawings

Fig. 1 is the glutinous No. 2 seed selection pedigree schematic diagrames of resisting stripe virus disease glutinous rice new varieties salt round-grained rice

The salt round-grained rice is paddy rice (Oryza sativa) glutinous No. 2, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on June 8th, 2009, and preserving number is: CGMCC No.3114.

Five, embodiment

Further specify resisting stripe virus disease glutinous rice variety breeding method of the present invention below in conjunction with accompanying drawing.

A) select with pressing down (No. 2, No. 2 * salt of town rice round-grained rice) F that rice forms with No. 2 hybridization of salt round-grained rice for No. 2 ₁Be female parent, (No. 8 * nasal mucus of the nasal mucus rice rice No. 9) F that forms with No. 9 hybridization of nasal mucus rice No. 8 and nasal mucus rice ₁Be male parent, sexual hybridization obtains F ₀Seed;

B) in 9 generations of selfing then,, selfing limit, limit is selected: select anti--middle bacterial blight-resisting, sick level 0-3 level; Anti--middle blast resisting, sick level 0-3 level; Anti-stripe virus disease is disease-resistant, sick level 0-2 level, incidence of disease 0-15%; The plant type compactness, blade is short very, falls the length 25-30cm of 1,2,3 leaves, thousand kernel weight 25-26 gram, the white rate of chalk≤30%, chalkiness degree≤5%, the strong fine individual plant of glutinous property;

From selfing F ₂Generation beginning, take the field to identify by generation and require: the plant type compactness, blade is very short, falls the length 25-30cm of 1,2,3 leaves, thousand kernel weight 25-26 gram; Identify exterior quality, the white rate of leading indicator chalk≤30%, chalkiness degree≤5% before the paddy maturation; Indoorly carrying out artificial threshing again, going out its glutinous property of rough evaluation again after the results, keeping the fine individual plant (fringe) that meets breeding objective and enter the seed selection work that next breeding generation repeats the previous generation again;

From selfing F ₃Generation beginning takes the capable 33cm+16.5cm of width, spacing in the rows 16.5cm, high fertile mu to execute the planting type that pure N17 kilogram, thin planting mu are planted 1.5 ten thousand caves, carries out artificial infection by generation and identifies bacterial leaf spot resistance, sick level index 0-3 level; Identify its rice blast resistance under field conditions (factors), sick level index 0-3 level; Carry out the stripe virus disease resistance under field conditions (factors) and identify, sick level index 0-2 level, incidence of disease index 0-15%;

9 generations of selfing are F ₉Glutinous No. 2 of resisting stripe virus disease glutinous rice variety salt round-grained rice have been bred, this kind 150-152 in the time of infertility days, plant height 90-95cm, blade is very short, promptly falls 1,2, the length of 3 leaves is 25-30cm, and the leaf look moderate, blade is straight and upright, and stem total leaf number 17-18 opens, 5 elongation internodes, sowing is to earing 105-108 days, and tillering ability is stronger, percentage of earbearing tiller 65-70%, general mu is fringe 20-22 ten thousand effectively, and spike length 18-21 centimetre, every total grain panicle 135-140 grain, the real grain of every fringe 125-130 grain, ripening rate 90-92%, thousand kernel weight 25-26 gram, no awns, all indexs of rice quality all reach GB quality glutinous rice standard, brown rice rate 83.3%, polished rice rate 71.7%, head rice rate 66.8%, grain length 5.0mm, disappear 6.3 grades of values of aspect ratio 1.8, alkali, glue denseness 100mm, amylose content 1.4%, glutinous property is strong, good palatability; Anti--middle bacterial blight-resisting, sick level 0-3 level; Anti--middle blast resisting resistance, sick level 0-3 level; High resisting-anti-stripe virus disease, sick level index 0-2 level, incidence of disease index 0-15%.

Table 1: the seed selection process that the salt round-grained rice of breeding method seed selection of the present invention is glutinous No. 2

The glutinous No. 2 culture technique main points of salt round-grained rice are as follows in addition:

Plant experimentally the output structure of glutinous No. 2 per mu yields of salt round-grained rice more than 600 kilograms through test: every mu 21.0 ten thousand fringe, 135 of every total grain panicles, ripening rate 90%, thousand kernel weight 25.5 grams.High-yield culturing should be given full play to the advantage of its big fringe, on the basis of suitable spike number, attacks big fringe guarantor grain and weighs.Promptly by cultivating the strong seedling of many tillers, rational close planting urgees early to send out early enough seedlings, and raising colony quality is accomplished fluently the high yield basis, and on the basis of guaranteeing suitable spike number, the short strong big fringe of bar is taken back again aim of high yield thereby reach big of fringe foot fringe.The technical essential that should grasp is:

(1) of the right age strong seedling is cultivated in optimum period light seeding

The salt round-grained rice belongs to slow ripe intermediate keng rice types glutinous No. 2, and generally with sowing in the first tenday period of a month in May, the length of time rice seedlings grow is controlled at about 30 days to well in slow ripe intermediate keng rice area.Watery ricenursery seeding quantity 25-30 kilogram/mu, non-irrigated seedling planting amount 35-40 kilogram/mu, land for growing field crops sowing quantity 2.5-3 kilogram/mu.The rice seedling bed will use sufficient base manure, vegetable soil, and it is fertile that a leaf is wholeheartedly executed wean, and tri-leaf period, benefit was executed relay fertilizer, and strengthened management, and especially non-irrigated seedling will be prevented blue or green withered, has controlled water, skilful fertilising, carries out the preventing and controlling of damage by disease and insect.Breed the of the right age strong seedling of delicate and pretty tall and straight anosis worm.

(2) rational close planting, an even surface planting improves and plants quality

Planting experimentally the result shows: in go up fertility field piece mu and plant 1.6-1.8 ten thousand caves, seeding row spacing 13cm * 28cm, every cave 1-2 this, every mu of basic seedling 5-6 ten thousand; The relatively poor field piece mu of fertility is planted 20,000 caves, seeding row spacing 13cm * 25cm, and every mu of basic seedling 7-8 ten thousand, comparatively favourable to colony with individual coordinated development, increasing ears and grains, be easier to obtain stable high yield.To improve conscientiously simultaneously and plant quality,, improve colony's quality, for striving many fringes, attacking big fringe and lay a solid foundation for early going out tiller, seedling enough early.

(3) science rich water is striven for high yield

600 kilograms of cultivations of glutinous No. 2 per mu yields of salt round-grained rice, the total fertilizer requirement of Honda is amounted to about 20 kilograms/mu of purity nitrogens.Phosphorus, potash fertilizer are used in collocation, and phosphorus, potash fertilizer are main as base manure.Fertilizer application is grasped the principle of " mend preceding heavy, middle control, back ", and account for 60% nitrogenous fertilizer early stage (base manure and tillering fertilizer), and fringe granulated fertilizer accounts for 40%.Water starch management should be taked an irrigation method of " shallow water transplants seedlings, very little water is lived, thin moisture tiller, water conservation heading flowering, alternation of wetting and drying ".Requirement was sent out foot in 15-20 days and is waited the fringe seedling planting the back, and when mu stem tiller reaches when waiting fringe seedling, the field is put in the draining gradation, and the control peak seedling is no more than 280,000/mu, become fringe 210,000/mu at last.Gathered in the crops ponding between drained field preceding about 7 days.

(4) preventing and controlling of reinforcement disease pest and weed

According to the pests occurrence rule of paddy rice disease, worm, crop smothering, in time carry out preventing and controlling, especially under the high-yield culturing condition,, should note carrying out preventing and controlling such as leaf-roller, paddy stem borer, rice fulgorid, banded sclerotial blight and weeds because nitrogen level is higher.

Claims (1)

1, a kind of breeding method of resisting stripe virus disease glutinous rice variety may further comprise the steps:

A) select with pressing down No. 2 F of No. 2 * salt of town rice round-grained rice that rice forms with No. 2 hybridization of salt round-grained rice for No. 2 ₁Be female parent, No. 9 F of No. 8 * nasal mucus of nasal mucus rice rice that form with No. 9 hybridization of nasal mucus rice No. 8 and nasal mucus rice ₁Be male parent, sexual hybridization obtains F ₀Seed;

B) in 9 generations of selfing then,, selfing limit, limit is selected: select anti--middle bacterial blight-resisting, sick level 0-3 level; Anti--middle blast resisting, sick level 0-3 level; Anti-stripe virus disease is disease-resistant, sick level 0-2 level, incidence of disease 0-15%; The plant type compactness, blade is short very, falls the length 25-30cm of 1,2,3 leaves, thousand kernel weight 25-26 gram, the white rate of chalk≤30%, chalkiness degree≤5%, the strong fine individual plant of glutinous property;

From selfing F ₂Generation beginning, take the field to identify by generation and require: the plant type compactness, blade is very short, falls the length 25-30cm of 1,2,3 leaves, thousand kernel weight 25-26 gram; Identify exterior quality, the white rate of leading indicator chalk≤30%, chalkiness degree≤5% before the paddy maturation; Indoorly carrying out artificial threshing again, going out its glutinous property of rough evaluation again after the results, keeping the fine individual plant fringe that meets breeding objective and enter the seed selection work that next breeding generation repeats the previous generation again;

From selfing F ₃Generation beginning takes the capable 33cm+16.5cm of width, spacing in the rows 16.5cm, high fertile mu to execute the planting type that pure N17 kilogram, thin planting mu are planted 1.5 ten thousand caves, carries out artificial infection by generation and identifies bacterial leaf spot resistance, sick level index 0-3 level; Identify its rice blast resistance under field conditions (factors), sick level index 0-3 level; Carry out the stripe virus disease resistance under field conditions (factors) and identify, sick level index 0-2 level, incidence of disease index 0-15%;

9 generations of selfing are F ₉Glutinous No. 2 of resisting stripe virus disease glutinous rice variety salt round-grained rice have been bred, this kind 150-152 in the time of infertility days, plant height 90-95cm, blade is very short, promptly falls 1,2, the length of 3 leaves is 25-30cm, and the leaf look moderate, blade is straight and upright, and stem total leaf number 17-18 opens, 5 elongation internodes, sowing is to earing 105-108 days, and tillering ability is stronger, percentage of earbearing tiller 65-70%, general mu is fringe 20-22 ten thousand effectively, and spike length 18-21 centimetre, every total grain panicle 135-140 grain, the real grain of every fringe 125-130 grain, ripening rate 90-92%, thousand kernel weight 25-26 gram, no awns, all indexs of rice quality all reach GB quality glutinous rice standard, brown rice rate 83.3%, polished rice rate 71.7%, head rice rate 66.8%, grain length 5.0mm, disappear 6.3 grades of values of aspect ratio 1.8, alkali, glue denseness 100mm, amylose content 1.4%, glutinous property is strong, good palatability; Anti--middle bacterial blight-resisting, sick level 0-3 level; Anti--middle blast resisting resistance, sick level 0-3 level; High resisting-anti-stripe virus disease, sick level index 0-2 level, incidence of disease index 0-15%.

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