CN1826873A - Seed-breeding and seed-producing method of hybrid rice II You 42 - Google Patents
Seed-breeding and seed-producing method of hybrid rice II You 42 Download PDFInfo
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- CN1826873A CN1826873A CNA200610037698XA CN200610037698A CN1826873A CN 1826873 A CN1826873 A CN 1826873A CN A200610037698X A CNA200610037698X A CN A200610037698XA CN 200610037698 A CN200610037698 A CN 200610037698A CN 1826873 A CN1826873 A CN 1826873A
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Abstract
The invention discloses a method for breeding and producing of hybrid seeds for hybrid rice II super 42. The breeding method comprises the following steps: A. taking Zhenhui129 as female parent and Zhenhui084 as male parent to get seed F0; B. auto-copulating for 5-7 generations and choosing at the same time plants with strong restoration and disease-resistant ability, short leaf, compact plant type and good appearance, cultivating out restoration system Zhenhui42 of new plant type, disease-resistant and high productivity; C. crossing sterile system II-32A with said restoration system Zhenhui42 and getting new rice breed II super 42 with high leaf blight-resistant property, short leaf and compact plant type. The rice got in this invention is characterized by the good comprehensive productivity, moderate maturity time; sound property; outstanding disease-resistant property and stable productivity.
Description
Technical field
The present invention relates to the breeding and the producing method for seed of a plant species, more specifically to breeding and the producing method for seed of a kind of hybrid rice II excellent 42.
Background technology
Paddy rice is the first crops of China, and the cultivated area of hybrid rice accounts for the over half of China's paddy rice cultivated area.The improvement of hybrid rice variety is the major fields of China's agri-scientific research always, and high-quality, high yield, resist are the important long-term targets of rice breeding more, and it is one of important channel of realizing breeding objective that ideotype and hybrid vigour combine.
Over past ten years, China's breeding of hybridized rice makes much progress, and large quantities of hybrid rice new varieties are applied on producing, but most hybrid rice new varieties blades are too in great numbers, and colony's improper ventilation causes colony's closing easily, damage by disease and insect takes place serious, is unfavorable for the raising of Rice Population output.Select that blade is short very, plant compactness, leaf growth speed in early stage hybrid rice varieties fast, that total leaf number is many be the effective way that solves colony and individual contradiction.
Retrieval is found, the Chinese patent application that application number is 94102551.9, the applying date is on March 18th, 1994 discloses the breeding method of a kind of hybrid rice, it is too in great numbers that yet the paddy rice of this patent application also exists blade, colony's improper ventilation, cause colony's closing easily, serious problem takes place in damage by disease and insect, and its rice quality is still waiting to improve.
Bacterial leaf-blight is a kind of important disease of Rice Production, if bacterial blight-resisting characteristic and novel strain type are combined, will further strengthen the extensive adaptability of hybrid rice and high yield, stable yields performance.
Summary of the invention
The objective of the invention is to solve above-mentioned problems of the prior art with not enough, a kind of breeding and producing method for seed with hybrid rice II excellent 42 of good novel strain type, disease-resistant, high yield is provided.
The applicant is through repetition test for many years, in many hybridization cultivation schemes and selection, find, breeding method of following hybrid rice and producing method for seed can well be realized goal of the invention to solve the problem that prior art exists, and breeding and its technical scheme of producing method for seed of hybrid rice II excellent 42 of the present invention are as follows:
The breeding method of hybrid rice II excellent 42 of the present invention may further comprise the steps:
A) select the plant type compactness, containing the fast elongated of growing thickly, to recover to be that special blue or green blood lineage's free good superior restorer line town extensive 129 does maternal, select the plant type compactness, contain the fast elongated of growing thickly recover be special blue or green blood lineage and carry Xa-7 bacterial leaf spot resistant ospc gene, colory free good superior restorer line town extensive 084 makes paternal hybrid and obtains F
0Seed;
B) in 5～7 generations of selfing,, selfing limit, limit is selected, and selects that restoring force is strong, disease-resistant, blade is short very, plant type compactness, the good individual plant of exterior quality, cultivate have good novel strain type, the superior restorer line town extensive 42 of disease-resistant, high yield;
C), obtain to have high resistance to hoja blanca, blade is shortly endured, excellent 42 (former name II excellent 085) of hybrid rice new varieties II of plant type compactness again with extensive 42 hybridization in male sterile line II-32A and above superior restorer line town.
The breeding method of above-mentioned hybrid rice seeds is from selfing 3 generation (F
3) begin, take the planting type of wide row, narrow strain, high fertilizer, thin planting, simultaneously in conjunction with the bacterial leaf-blight inoculation, carry out the bacterial leaf spot resistance evaluation, from F
4Begin to measure its restoring force and general combining ability with Zhenshan 97a; The planting type of described wide row, narrow strain, high fertilizer, thin planting, its line width are that 33cm, spacing in the rows are that 16.5cm, high fertilizer are 1.5 ten thousand seedling/mus for every mu of 17 kilograms of pure N, thin planting.
The breeding method of above-mentioned hybrid rice II excellent 42 is from selfing 4 generation (F
4) begin to join hybridization with the Zhenshan 97a survey, measure its restorability.
The producing method for seed of hybrid rice II excellent 42 of the present invention, it is maternal to be to use II-32A to do, and presses down extensive 42 and makes male parent, hybridizes preparing hybrid rice II excellent 42.
The producing method for seed of described hybrid rice II excellent 42 is: male parent is divided the sowing of two phases, I phase male parent is sowed by the end of April, II phase male parent was than I phase male parent late sowing 10 days, maternal than I phase male parent late sowing 14 days, seed farm father and mother one's own profession ratio is 2: 12, I, single this alternate the planting of II phase male parent, maternal seeding row spacing 13cm * 16.5cm lacks and originally plants.The artificial supplementary pollination measure is taked in the production of hybrid seeds simultaneously, and carries out diseases and pests controlling work.
Extensive 42 (former name towns extensive 085), town of the present invention have following beneficial effect:
About the 140 days time of infertility, about 110 centimetres of plant heights, about total blade 20 leaves of stem, plant type is good, blade is tall and straight, and the leaf look moderate, and tillering ability is strong, and the fringe type is bigger, spike length is about in the of 24 centimetres, and about 160 of every total grain panicles, ripening rate are more than 93%, and thousand kernel weight 31 restrains, rice quality is excellent, and cane is sturdy, and lodging resistance is good, and restoring force is strong, extensive spectrum is wide, and the coordinate force height is to bacterial leaf-blight immunity, high blast resisting, it is smooth to open grain husk, and the pollen amount foot is beneficial to the raising hybrid seed yield.
Hybrid rice II excellent 42 of the present invention in addition has following beneficial effect:
1. comprehensive yielding ability is good, and the ripe phase is more moderate
Excellent 42 tillering abilities of II are stronger, percentage of earbearing tiller height, big panicle many grains per panicle, fecundity is good, general every mu of effective fringe 16-18 ten thousand, about 24 centimetres of spike lengths, every total grain panicle is several more than 160, ripening rate is more than 86%, about thousand kernel weight 29.5 grams, all shows higher yield level and yield potential in pilot experiment and demonstration are planted experimentally.About the 142 days time of infertility, late-maturing about 3 days approximately than Shanyou 63.Newly make up variety comparative test simultaneously, folding per mu yield 689.89kg, comparison is according to Shanyou 63 volume increase 19.30%.
2. best in quality, commodity is good
Excellent 42 grain of II are parallel shape, and are slightly elongated and full, yellow.Sample presentation to Ministry of Agriculture's rice and quality of item supervision and inspection center detected in 2002: the long 6.2mm of the grain of rice, aspect ratio 2.4, brown rice rate 80.3%, polished rice rate 73.0%, head rice rate 54.5%, the white rate 28% of chalk, chalkiness degree 4.9%, 2 grades of transparencies, disappear 4.8 grades of values of alkali, glue denseness 58mm, amylose content 22.7%.
3. plant type is good, easily cultivation
Excellent 42 bud scales of II are short and thick, are aubergine, and the seedling body is strong and vigorous, and leaf is tall and straight, the leaf look medium, and tillering ability is stronger, and plant type is collecting and distributing moderate, the stem number of blade 18 leaves, 5 elongation internodes, ear node is long, about 120 centimetres of plant heights, cane is sturdy, and the leaf sheath plumpness is embraced the stem consolidation, do not reveal joint, upper blade is tall and straight, is subjected to the light attitude good, functional period is long, and it is delicate and pretty to grow, and self regulating power is strong.
4. disease resistance is outstanding, and yield stability is good
Contain dominance wide spectrum high resistance to hoja blanca gene Xa-7 and bacterial leaf spot resistant gene Xa-4 because of pressing down extensive 42, so II excellent 42 has high resistance to hoja blanca and rice blast.Identify according to the Institute of Plant Protection's artificial infection of academy of agricultural sciences, Jiangsu Province: the resistance to the KS-6-6 of bacterial leaf-blight, Zhejiang 173, PX079, four bacterial classifications of JS49-6 is respectively 1,2,1,1 grade (9 grades of systems); With middle D1, middle E3, middle B5, middle G1, five rice blast biological strain inoculations of middle F1, sick level is 0 grade entirely.And it is light not have stripe virus disease, banded sclerotial blight and other mixed natural disposition diseases, ripe lover of later stage.Because excellent 42 canes of II are sturdy, leaf sheath is embraced the stem consolidation, has fertilizer resistance anti-lodging performance preferably.
Description of drawings
Fig. 1 is the excellent 42 breeding pedigree schematic diagrames of hybrid rice II of the present invention
Embodiment
Further specify breeding and the producing method for seed of hybrid rice II excellent 42 of the present invention below in conjunction with accompanying drawing.
Breeding method:
A) select that plant type is compact, containing the fast elongated of growing thickly, to recover to be that special blue or green blood lineage's free good superior restorer line town extensive 129 does maternal, select plant type compact, contain the fast elongated of growing thickly recover be special blue or green blood lineage and carry Xa-7 bacterial leaf spot resistant ospc gene, colory free good superior restorer line town extensive 084 makes paternal hybrid and obtains F
0Seed;
B) in 5 generations of selfing then,, selfing limit, limit is selected, and selects that disease-resistant, blade is shortly endured, plant type compactness, the good individual plant of exterior quality, from selfing 3 generation F
3Begin, take the planting type of wide row (33cm+16.5cm), narrow strain (16.5cm), high fertile (mu is executed pure N17 kilogram), thin planting (mu is planted 1.5 ten thousand caves), the while carries out bacterial leaf spot resistance and identifies, from selfing 4 generation (F in conjunction with the bacterial leaf-blight inoculation
4) beginning surveys with Zhenshan 97a and joins hybridization, cultivate have good novel strain type, extensive 42 (former name towns extensive 085), superior restorer line town disease-resistant, high yield;
C), obtain to have high resistance to hoja blanca, blade is shortly endured, the hybrid rice II excellent 42 of plant type compactness again with produce going up widely used male sterile line II-32A and extensive 42 hybridization in above superior restorer line hybridization long-grained nonglutinous rice town.Concrete breeding is through seeing Table 1.
The propagation method (Jurong, Jiangsu) of male sterile line II-32A is simultaneously: sow for maternal (II-32A) late April to the first tenday period of a month in May, male parent (II-32B) divided for two phases, the more maternal late sowing of I phase male parent 3 days, the more maternal late sowing of II phase male parent 7 days, father and mother's one's own profession was than 2: 9, about maternal seeding row spacing 13cm * 16.5cm, I, alternate the planting of II phase male parent, father and mother originally are individual plant transplanting.The strict isolation, note anti-assorted impurity elimination; Take ancillary method, improve outcrossing seed-setting rate.
The producing method for seed of II of the present invention excellent 42 (Jurongs, Jiangsu):
Make female parent with II-32A, press down extensive 42 and make male parent.Male parent is divided the sowing of two phases, I phase male parent is sowed by the end of April, II phase male parent was than I phase male parent late sowing 10 days, maternal than I phase male parent late sowing 14 days, leaf age differs from about 2.1 leaves, and seed farm father and mother one's own profession ratio is 2: 12, I, single this alternate the planting of II phase male parent, about maternal seeding row spacing 13cm * 16.5cm, lack and originally plant.The strict isolation, note impurity elimination, take the artificial supplementary pollination measure, spray in right amount " 920 ", and note carrying out diseases and pests controlling work.
Table 1: breeding method seed selection process of the present invention
| Time, place | Breeding and selfing | Pedigree | Process and explanation |
| Spring Hainan in 1997 | Combo | Press down extensive 129 * town extensive 084 | Get 72 seeds, combination number friendship 294 |
| Jurong in 1997 | F 1 | 2837 | Broadcast 60 seeds, become seedling 40 strains, plant 24 strains, receive two strains after the impurity elimination, get 40 gram seeds |
| Jurong in 1998 | F 2 | 684 | Broadcast 35 gram seeds, plant 1072 strains, by the strain screening, be selected in 11 individual plants according to comprehensive agronomy proterties and exterior quality etc. |
| Spring Hainan in 1999 | F 3 | 9025 | Eliminate 5 systems, selected 20 individual plants are from F 3Beginning to carry out bacterial leaf spot resistance by generation identifies. |
| Jurong in 1999 | F 4 | 591 | Selecting 13 systems to survey with Zhenshan 97a respectively joins. |
| Jurong in 2000 | F 5 | 258 | Selecting 4 systems to survey with Zhenshan 97a joins again |
| Calendar year 2001 the Jurong | F 6 | C042 | Survey with II-32A again and join |
| Spring Hainan in 2002 | F 7 | 9085 | Further purify and the manual production of hybrid seeds |
| 2002 | Produce breeder's stock | The Xian that mixes newly makes up variety comparative test | |
| 2003 | Produce breeder's stock, original seed | II excellent 42 participates in the whole nation assorted Xian district examination |
The excellent 42 culture technique main points of II are as follows in addition:
Plant experimentally the output structure of the excellent 42 per mu yield 650Kg of II through test: every mu 17.0 ten thousand fringe, every fringe are more than 160, and ripening rate is more than 85%, about thousand kernel weight 29.5 grams.High-yield culturing should be given full play to the advantage of its big fringe, on the basis of suitable spike number, attacks big fringe guarantor grain and weighs.Promptly by cultivating the strong seedling of many tillers, rational close planting urgees early to send out early enough seedlings, and raising colony quality is accomplished fluently the high yield basis, and on the basis of guaranteeing suitable spike number, the short strong big fringe of bar is taken back again aim of high yield thereby reach big of fringe foot fringe.The technical essential that should grasp is:
1. of the right age strong seedling is cultivated in optimum period light seeding
During II excellent 42 belongs to ripe in the Xian type, generally with sowing in the first tenday period of a month in May, the length of time rice seedlings grow is controlled at about 30 days to well in southern area of Jiangsu Province.The every mu of rice seedling bed amount of broadcasting 10-15 kilogram; About 30 kilograms of every mu of rice seedling bed of drought seedling amounts of broadcasting; Every mu of land for growing field crops is with planting 1.0 kilograms.The rice seedling bed will use sufficient base manure, vegetable soil, and it is fertile that a leaf is wholeheartedly executed wean, and tri-leaf period, benefit was executed relay fertilizer, and strengthened management, and especially non-irrigated seedling will be prevented blue or green withered, has controlled water, skilful fertilising, carries out the preventing and controlling of damage by disease and insect.Breed the of the right age strong seedling of delicate and pretty tall and straight anosis worm.
2. rational close planting, an even surface planting improves and plants quality
Plant experimentally and show: in go up fertility field piece mu and plant 1.6～1.8 ten thousand caves, seeding row spacing 13cm * 28cm, every cave 1-2 this, every mu of basic seedling about 50,000; The relatively poor field piece mu of fertility is planted 20,000 caves, seeding row spacing 13cm * 25cm, and every mu of basic seedling about 70,000, comparatively favourable to colony with individual coordinated development, increasing ears and grains, be easier to obtain stable high yield.To improve conscientiously simultaneously and plant quality,, improve colony's quality, for striving many fringes, attacking big fringe and lay a solid foundation for early going out tiller, seedling enough early.
3. science rich water is striven for high yield
The yield level of II excellent 42 is higher than Shanyou 63, needs rich water flat also a little more than Shanyou 63.General mu is executed about 15 kilograms of purity nitrogens, and thin field also should increase in right amount.Short before fertilizer application is grasped, middle control, the steady principle in back.Requirement uses sufficient base manure (base manure is as far as possible based on fertilizer, and nitrogen, phosphorus, potassium cooperate), early executes the heavy dressing fetilizer for tillering; send out short morning; early reach seedling, in time put the field, on the basis of middle control; executed ear manuer; short guarantor takes into account, and based on short, the later stage is adopted the wet method of doing; note not cutting off the water supply too early, guarantee the ripe high yield and high quality of living.
4. strengthen the preventing and controlling of disease pest and weed
According to the pests occurrence rule of disease, worm, crop smothering, in time carry out preventing and controlling, especially under the high-yield culturing condition,, should note carrying out the preventing and controlling of leaf-roller, paddy stem borer, rice fulgorid and weeds because nitrogen level is higher.
Claims (7)
1, the breeding method of a kind of hybrid rice II excellent 42 may further comprise the steps:
A) select the plant type compactness, containing the fast elongated of growing thickly, to recover to be that special blue or green blood lineage's free good superior restorer line town extensive 129 does maternal, select the plant type compactness, contain the fast elongated of growing thickly recover be special blue or green blood lineage and carry Xa-7 bacterial leaf spot resistant ospc gene, colory free good superior restorer line town extensive 084 makes paternal hybrid and obtains F
0Seed;
B) in 5～7 generations of selfing,, selfing limit, limit is selected, and selects that restoring force is strong, disease-resistant, blade is short very, plant type compactness, the good individual plant of exterior quality, cultivate have good novel strain type, the superior restorer line town extensive 42 of disease-resistant, high yield;
C), obtain to have high resistance to hoja blanca, blade is shortly endured, the hybrid rice new varieties II excellent 42 of plant type compactness again with extensive 42 hybridization in male sterile line II-32A and above superior restorer line town.
2, the breeding method of hybrid rice II excellent 42 according to claim 1 is characterized in that: from selfing 3 generation (F
3) begin, take the planting type of wide row, narrow strain, high fertilizer, thin planting, simultaneously in conjunction with the bacterial leaf-blight inoculation, carry out the bacterial leaf spot resistance evaluation, from F
4Begin to measure its restoring force and general combining ability with Zhenshan 97a.
3, the breeding method of hybrid rice II excellent 42 according to claim 2, it is characterized in that: the planting type of described wide row, narrow strain, high fertilizer, thin planting, its line width are that 33cm, spacing in the rows are that 16.5cm, high fertilizer are 1.5 ten thousand seedling/mus for every mu of 17 kilograms of pure N, thin planting.
4, the breeding method of hybrid rice II excellent 42 according to claim 1 is characterized in that: from selfing 4 generation (F
4) begin to join hybridization with the Zhenshan 97a survey, measure its restorability.
5, the breeding method of hybrid rice II excellent 42 according to claim 1 is characterized in that step is as follows:
A) select the plant type compactness, containing the fast elongated of growing thickly, to recover to be that special blue or green blood lineage's free good superior restorer line town extensive 129 does maternal, select the plant type compactness, contain the fast elongated of growing thickly recover be special blue or green blood lineage and carry Xa-7 bacterial leaf spot resistant ospc gene, colory free good superior restorer line town extensive 084 makes paternal hybrid and obtains F
072 in seed;
B) selfing F
1, broadcast 60 seeds, become seedling 40 strains, plant 24 strains, receive two strains after the impurity elimination, get 40 gram seeds; Selfing F
2, broadcast 35 gram seeds, plant 1072 strains, by the strain screening, be selected in 11 individual plants according to comprehensive agronomy proterties and exterior quality etc.; Selfing F
3, eliminate 5 systems, selected 20 individual plants are from F
3Beginning to carry out bacterial leaf spot resistance by generation identifies; Selfing F
4, select 13 systems to survey with Zhenshan 97a respectively and join; Selfing F
5, select 4 systems to survey with Zhenshan 97a again and join, cultivate have good novel strain type, the superior restorer line town extensive 42 of disease-resistant, high yield;
C), obtain to have high resistance to hoja blanca, blade is shortly endured, the hybrid rice new varieties II excellent 42 of plant type compactness again with extensive 42 hybridization in male sterile line II-32A and above superior restorer line town.
6, the producing method for seed of hybrid rice II excellent 42 according to claim 1 is characterized in that: it is maternal to use II-32A to do, and presses down extensive 42 and makes male parent, hybridizes preparing hybrid rice II excellent 42.
7, the producing method for seed of hybrid rice II excellent 42 according to claim 6, it is characterized in that: male parent is divided the sowing of two phases, I phase male parent is sowed by the end of April, II phase male parent was than I phase male parent late sowing 10 days, maternal than I phase male parent late sowing 14 days, seed farm father and mother one's own profession ratio is 2: 12, I, single this alternate the planting of II phase male parent, maternal seeding row spacing 13cm * 16.5cm lacks and originally plants.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101796918A (en) * | 2009-03-12 | 2010-08-11 | 江苏丘陵地区镇江农业科学研究所 | Breeding and seed production methods of quality multi-resistant high-yield hybrid rice |
| CN103461094A (en) * | 2013-08-14 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Method for breeding indica rice restorer line with wide capability and bacterial leaf blight resistance |
| CN103461093A (en) * | 2013-08-14 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Intersubspecific indica type three-line hybrid rice restorer line breeding method |
| CN103518618A (en) * | 2013-10-14 | 2014-01-22 | 广西恒茂农业科技有限公司 | Method for breeding rice hybrid Lanyou2098 |
| CN115623983A (en) * | 2022-10-19 | 2023-01-20 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of rice with competitive advantage |
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- 2006-01-10 CN CNA200610037698XA patent/CN1826873A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101796918A (en) * | 2009-03-12 | 2010-08-11 | 江苏丘陵地区镇江农业科学研究所 | Breeding and seed production methods of quality multi-resistant high-yield hybrid rice |
| CN103461094A (en) * | 2013-08-14 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Method for breeding indica rice restorer line with wide capability and bacterial leaf blight resistance |
| CN103461093A (en) * | 2013-08-14 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Intersubspecific indica type three-line hybrid rice restorer line breeding method |
| CN103461093B (en) * | 2013-08-14 | 2015-09-02 | 江苏丘陵地区镇江农业科学研究所 | A kind of breeding method of subspecies Indica Three Line Restorer |
| CN103518618A (en) * | 2013-10-14 | 2014-01-22 | 广西恒茂农业科技有限公司 | Method for breeding rice hybrid Lanyou2098 |
| CN115623983A (en) * | 2022-10-19 | 2023-01-20 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of rice with competitive advantage |
| CN115623983B (en) * | 2022-10-19 | 2023-11-21 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of rice with competitive advantage |
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