CN103250635A - Breeding method and seed production method of high-grain-weight and high-quality two-line hybrid rice - Google Patents

Breeding method and seed production method of high-grain-weight and high-quality two-line hybrid rice Download PDF

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CN103250635A
CN103250635A CN2013102153971A CN201310215397A CN103250635A CN 103250635 A CN103250635 A CN 103250635A CN 2013102153971 A CN2013102153971 A CN 2013102153971A CN 201310215397 A CN201310215397 A CN 201310215397A CN 103250635 A CN103250635 A CN 103250635A
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rice
grain
quality
salt
hybrid rice
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CN103250635B (en
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姚立生
孙明法
严国红
王爱民
朱国永
唐红生
何冲霄
任仲玲
刘凯
张桂云
施伟
单忠德
孙红芹
万林生
倪正斌
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YANCHENG MINGTIAN SEED SCIENCE AND TECHNOLOGY Co Ltd
Jiangsu Institute of Coastal Area Agricultural Sciences
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YANCHENG MINGTIAN SEED SCIENCE AND TECHNOLOGY Co Ltd
Jiangsu Institute of Coastal Area Agricultural Sciences
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Abstract

The invention discloses a breeding method and a seed production method of high-grain-weight and high-quality two-line hybrid rice, belonging to the technical field of plant breeding. The breeding method comprises the steps of: selecting natural variants which are slightly large in increment, slightly high in plant, slightly large in ear type and slightly high in grain weight from a Guangzhan 63S growth population group, wherein the photo-thermo sensitive genic male sterile line characters of the natural variants are similar to those of the Guangzhan 63S; from the F2 generation, in contrast with the Guangzhan 63S for each generation, selecting excellent single plants which have the photo-thermo sensitive genic male sterile line characters, the quality and the disease resistance similar to those of the Guangzhan 63S, are slightly large in growth and slightly large in ear type until indica type photo-thermo sensitive genic male sterile line salts, 220 S which are compact in plant type, strong in disease resistance, high in combining ability and high in quality, are bred in the F6 generation, wherein the thousand seed weight of the excellent single plants is about 29g and the thousand seed weight of the indica type photo-thermo sensitive genic male sterile line salts 220 S is 29g; and then preparing a hybrid rice combination from the salts 220S and excellent restorers Yanhui 888 to obtain Yanliangyou 2208 as a new two-line hybrid indica rice variety, which is compact in plant type, moderate in harvesting period and high in grain weight, and resists rice blast and bacterial blight, wherein the quality of the Yanliangyou 2208 reaches the third-grade national standard.

Description

A kind of breeding method and producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind
One, technical field
The present invention relates to a kind of breeding method and producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind, belong to field of plant variety breeding technology, breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind of more specifically saying so.
Two, background technology
Paddy rice is the staple food crop of China, and the cultivated area of hybrid rice accounts for the over half of China's paddy rice cultivated area.The improvement of double-linear hybrid rice kind is the major fields of China's agri-scientific research in the recent period, and high-quality, high yield, many anti-, wide fitting are the important long-term targets of rice breeding.It is one of important channel of realizing China's breeding of hybridized rice third phase target that the ideotype that Yuan Longping academician proposes and hybrid vigour combine.
Over past ten years, China's breeding of hybridized rice research has obtained the achievement that makes the world attract attention, having bred super double-linear hybrid rices such as " two line system ", " two excellent 0293 ", " Y two excellent No. 2 " in succession newly makes up, and realizing the target of China's hundred mu of first, second and third phases of high yield side's per mu yield of super rice, sign China's rice breeding and production continue to keep world lead level.China's super hybrid rice realizes that the breeding objective of first, second and third phase also is the yield level in hundred mu of sides and thousand mu of high yield sides, and the yield level that China's paddy rice large tracts of land is produced has realized that just per mu yield exceedes 500 kilograms, and average yield per mu does not also reach 550 kilograms of levels.For many years, domestic and international many colleague's researchs draw, and increasing thousand kernel weight is one of important channel of further improving rice varieties yield per unit area.At present, inharmonic defectives such as the super double-linear hybrid rice new varieties ubiquity high-quality of using in the production, output heavy with high grain and high-quality.The high grain of seed selection is heavy and reach national fine quality rice standard, the good hybrid rice new varieties of other bulk properties are further to improve China's hybrid rice production output and meet the effective way that high-quality resists more.
Three, summary of the invention
Technical problem
The objective of the invention is to solve above-mentioned problems of the prior art with not enough, the applicant is through experimental study for many years, in many hybridization cultivation schemes and selection, find, breeding and the producing method for seed of the heavy excellent double-linear hybrid rice of a kind of high grain, can well realize the problem that goal of the invention exists with the solution prior art, and successfully select the double-linear hybrid rice new varieties salt two excellent 2208 that high grain is heavy, rice quality reaches three grades of superior rice standards of GB.
Technical scheme
Breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind provided by the invention may further comprise the steps:
A) extensively account for the 63S kind at high-quality, blast resisting, the indica type photo-thermo-sensitive genic male sterile line of high-combining ability and plant bigger, slightly height, bigger, heavily slightly height, sterile strain 1 strain of natural variation similar to extensively accounting for 63S of photo-thermo-sensitive genic male sterile line characteristic of grain of fringe type of plant of acquisition amount of growth in the colony, collect this natural variation strain seed (F when cutting root and stem of certain plants regeneration maturation 1);
B) selfing limit in limit is selected then, from selfing 2 generation (F 2) beginning, it is contrast that every Dai Jun extensively accounts for 63S with indica type photo-thermo-sensitive genic male sterile line, and selective light temperature-sensitive sterile line characteristic, quality, disease resistance be with extensively to account for 63S similar, and amount of growth is bigger, the fringe type is bigger, the fine individual plant of thousand kernel weight 29 about restraining, to selfing 6 generation (F 6) breed that plant type compactness, disease resistance are strong, the indica type photo-thermo-sensitive genic male sterile line salt 220S of high-combining ability, high-quality, thousand kernel weight 29 grams;
Indica type photo-thermo-sensitive genic male sterile line salt 220S and two with CGMCCNO.7558 is that recovery is the extensive 888 preparing hybrid rice combination of salt, obtains high-grain weight excellent-quality double-linear hybrid rice new varieties.
Breed high-grain weight excellent-quality double-linear hybrid rice new varieties, name to salt two excellently 2208, it is characterized in that:
Salt two excellent 2208 seedling are short strong, the short and thick green of bud scale, and blade is straight and upright, and the leaf look dark green, and stooling is stronger; Become the strain plant type collecting and distributing moderate, the average 125cm of plant height, the total blade 17-18 of stem opens, 5 elongation internodes, three functional leafs in top are straight and upright, are subjected to the light attitude good, lift to be the straw mulch top the blue or green seed yellow maturity lover of bar when ripe on the sword-like leave; The percentage of earbearing tiller is average 65%, and general mu is fringe 15-17 ten thousand effectively, and average mu is fringe 15.8 ten thousand effectively, and the fringe type is bigger, the average 25.4cm of spike length, and average 168.5 of every total grain panicle number, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, average 30 grams of thousand kernel weight; Average 139.5 days of the time of infertility than contrast II excellent 838 ripe 5 days late, belonged to the basic nutrition vegetative form, in ripe in Xian two-line hybrid rice type.
The producing method for seed of described high-grain weight excellent-quality double-linear hybrid rice new varieties is characterized in that:
The producing method for seed of salt two excellent 2208 is the sowings of extensive 888 minutes two phases of male parent salt, I phase male parent is sowed by the end of April, II phase male parent was than I phase male parent late sowing 10 days, maternal than I phase male parent late sowing 35-36 days, this line number of seed farm father and mother is than being 2:12, I, single this alternate the planting of II phase male parent, maternal seeding row spacing 13cm * 16.5cm, every cave 2-3 originally plants the basic seedling of foot.
Beneficial effect
Compared with prior art, the breeding of a kind of high-grain weight excellent-quality double-linear hybrid rice of the present invention kind and producing method for seed have following advantage and good effect:
1, adopts the breeding method of a kind of high-grain weight excellent-quality ternary hybrid rice variety provided by the present invention, breed the indica type photo-thermo-sensitive genic male sterile line salt of high-grain weight excellent-quality 220S.
Salt 220S seedling is short strong, and blade is slightly wide, and blade is endured, leaf look light green, tillering ability is stronger, and property in great numbers is good, become the strain plant type collecting and distributing moderate, about plant height 85cm, 6 internodes, the total blade 15-17 of stem leaf, stooling is stronger, single-strain tassel number 8-10, about spike length 23.0cm, the total grain husk flower of every fringe 150-160 grain, grain length 6.5mm, aspect ratio 2.8, thousand kernel weight 29 gram, the rice quality leading indicator reaches GB superior rice standard more than three grades.The paddy look slightly dark, clever sharp bar yellow, and no tip awn, the fringe top is degenerated slightly heavy.
Salt 220S is sterile, and the phase is longer, and about 50 days stable sterile phases, sterile phase pollen abortion type is no pollen or a little abortive pollen type, and sterile plant rate reaches 100% in the sterile phase, and the pollen sterility degree is 100%, and self-fruitful rate is 0%.Sterility in the sterile phase and the fertility restorer that can educate in the phase are all better.2012, identify according to China Paddy Rice Inst's climatic cabinate fertility: salt 220S sterility is stable, and fertility conversion origin temp is lower, and under 14.5h long-day condition, critical male sterile temperature is lower than 24 ℃; Under 11.5h short-day condition, critical male sterile temperature is lower than 24 ℃.
Salt 220S is used for sowing about the hybrid rice production of hybrid seeds general June 5, heading mid-August, general duration from seeding to heading 84-85 days, the 115-117 days time of infertility in Yancheng, Jiangsu Province area.Belong to Xian male sterile line type in the precocity.Under the normal weather of early and middle ten days in August, the 9:00 beginning in the morning is spent, and 10:30-11:30 enters full-bloom stage, opens grain husk and lasts 30~45 minutes to closing grain husk, and single tassel blossom is gone through 4~5 days phases, and colony blooms and goes through phase 8-9 days.Column cap is colourless, stigma exposing ratio 78.3%, and bilateral exposing ratio 43.5% wherein, outcrossing seed-setting rate about 46.6%, responsive to " 920 ", different friendship is good, about 200 kilograms of general mu hybrid seed yields, high-yield field piece mu hybrid seed yield is more than 300 kilograms.
2, adopt breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind provided by the present invention, breed high-grain weight excellent-quality double-linear hybrid rice new varieties salt two excellent 2208.
A) stable high yield, yield potentiality is big
2010-2011, salt two excellent 2208 is participated in China's middle and lower reach of Yangtze River medium rice district multiple spot variety comparative test, and 579.41 kilograms of two annual per mu yields are than excellent 838 volume increase 4.73% of contrast II, than the excellent 838 volume increase point of contrast II ratio 77.9%; Than contrast rich two excellent No. 4 volume increase 3.84%, than contrast rich two excellent No. 4 volume increase point ratios 73.3%, all reach utmost point significance level.
Participated in China middle and lower reach of Yangtze River medium rice district multiple spot Da Qu variety comparative test in 2012,571.39 kilograms of average yield per mus are than excellent 838 volume increase 8.15% of contrast II, than the excellent 838 volume increase point of contrast II ratio 100%.
B) best in quality, commodity is good
Salt two excellent 2208 grain are elongated, slightly short awns, and it is yellow that grain is, and plumpness is good, and grain of rice transparency is good.Detected through Ministry of Agriculture's rice and quality of item supervision and inspection center in 2011: brown rice rate 81.3%, polished rice rate 73.4%, head rice rate 65.4%, grain length 7.1mm, aspect ratio 3.0, the white rate 28.0% of chalk, chalkiness degree 3.5%, 1 grade of transparency, disappear 7.0 grades of values of alkali, glue denseness 79mm, amylose content 17.4%, all indexs of rice quality all reach three grades of GBs, standard laid down by the ministries or commissions of the Central Government secondary superior rice standard.
C) disease resistance is outstanding, and yield stability is good
2010-2011 carried out disease resistance in continuous 2 years to be identified, salt two excellent 2208 pairs of rice blast, bacterial leaf spot resistance obviously are better than check variety II excellent 838, similar to brown planthopper resistance and check variety II excellent 838, its specific targets are: 5.7 grades of rice blast composite indexes (CK7.2 level), highest 9 grades (the CK9 level) of fringe pest percent of loss; 5 grades of bacterial leaf-blight average levels (CK7 level), highest 5 grades (CK7 level); 8 grades of brown planthopper average levels (CK9 level), highest 9 grades (CK9 level).Rice blast, bacterial leaf-blight and brown planthopper resistance are identified with reference to national new rice variety pilot experiment standard: 0 grade (high anti-), 1 grade (resist), 3 grades (in anti-), 5 grades (middle sense), 7 grades (sense), 9 grades (height is felt).Because salt two excellent 2208 canes are sturdy, leaf sheath is embraced the stem consolidation, has fertilizer resistance anti-lodging performance preferably.
D) economical character is good, wide adaptability
Salt two excellent 2208 seedling are short strong, the short and thick green of bud scale, and blade is straight and upright, and the leaf look dark green, and stooling is stronger.Become the strain plant type collecting and distributing moderate, about plant height 125cm, the total blade 17-18 of stem opens, 5 elongation internodes, and three functional leafs in top are straight and upright, are subjected to the light attitude good, lift to be the straw mulch top the blue or green seed yellow maturity lover of bar when ripe on the sword-like leave.About the percentage of earbearing tiller 65%, general mu is fringe 15-17 ten thousand effectively, and average mu is fringe 15.8 ten thousand effectively, and the fringe type is bigger, spike length 25.4cm, and several about 168.5 of every total grain panicle, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, thousand kernel weight 30 grams.Than contrast II excellent 838 ripe 5 days late, belong to the basic nutrition vegetative form the 139.5 days time of infertility, in ripe in Xian double-linear hybrid rice type.
Four, description of drawings
Accompanying drawing 1: be high-grain weight excellent-quality double-linear hybrid rice kind salt two excellent 2208 breeding pedigree schematic diagrames of the present invention
Biological preservation
Salt 220S, classification called after paddy rice (Oryzasativa), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 6th, 2013, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, preserving number is: CGMCCNo.7558.
Five, embodiment
Further specify a kind of breeding and producing method for seed of high-grain weight excellent-quality double-linear hybrid rice kind below in conjunction with accompanying drawing 1.
1, breeding method
A) extensively account for the 63S kind at high-quality, blast resisting, the indica type photo-thermo-sensitive genic male sterile line of high-combining ability and plant bigger, slightly height, bigger, heavily slightly height, sterile strain 1 strain of natural variation similar to extensively accounting for 63S of photo-thermo-sensitive genic male sterile line characteristic of grain of fringe type of plant of acquisition amount of growth in the colony, collect this natural variation strain seed (F when cutting root and stem of certain plants regeneration maturation 1);
B) selfing limit in limit is selected then, from selfing 2 generation (F 2) beginning, it is contrast that every Dai Jun extensively accounts for 63S with indica type photo-thermo-sensitive genic male sterile line, and selective light temperature-sensitive sterile line characteristic, quality, disease resistance be with extensively to account for 63S similar, and amount of growth is bigger, the fringe type is bigger, the fine individual plant of thousand kernel weight 29 about restraining, to selfing 6 generation (F 6) breed that plant type compactness, disease resistance are strong, the indica type photo-thermo-sensitive genic male sterile line salt 220S of high-combining ability, high-quality, thousand kernel weight 29 grams;
C) again with more than the indica type photo-thermo-sensitive genic male sterile line salt 220S that breeds and Jiangsu Inst. of Coastal Area Agricultural Sciences breed have plant type compactness, high-quality, extensive 888 a preparing hybrid rice combination of superior restorer line salt disease-resistant, high yield, obtain plant type compactness, suitable middle and high grain of ripe phase is heavy, meter matter reaches three grades of superior rices of GB, middle blast resisting and bacterial leaf-blight double-line hybrid long-grained nonglutinous rice new varieties salt two excellent 2208.
Concrete breeding is through seeing Table 1.
Table 1: high-grain weight excellent-quality double-linear hybrid rice kind salt two excellent 2208 breedings processes of the present invention
Figure BDA00003282372100051
Adopt breeding and the producing method for seed of a kind of high-grain weight excellent-quality double-linear hybrid rice kind provided by the present invention, breed high-grain weight excellent-quality double-linear hybrid rice new varieties salt two excellent 2208.
A) stable high yield, yield potentiality is big
2010-2011, salt two excellent 2208 is participated in China's middle and lower reach of Yangtze River medium rice district multiple spot variety comparative test, and 579.41 kilograms of two annual per mu yields are than excellent 838 volume increase 4.73% of contrast II, than the excellent 838 volume increase point of contrast II ratio 77.9%; Than contrast rich two excellent No. 4 volume increase 3.84%, than contrast rich two excellent No. 4 volume increase point ratios 73.3%, all reach utmost point significance level.
Participated in China middle and lower reach of Yangtze River medium rice district multiple spot Da Qu variety comparative test in 2012,571.39 kilograms of average yield per mus are than excellent 838 volume increase 8.15% of contrast II, than the excellent 838 volume increase point of contrast II ratio 100%.
B) best in quality, commodity is good
Salt two excellent 2208 grain are elongated, slightly short awns, and it is yellow that grain is, and plumpness is good, and grain of rice transparency is good.Detected through Ministry of Agriculture's rice and quality of item supervision and inspection center in 2011: brown rice rate 81.3%, polished rice rate 73.4%, head rice rate 65.4%, grain length 7.1mm, aspect ratio 3.0, the white rate 28.0% of chalk, chalkiness degree 3.5%, 1 grade of transparency, disappear 7.0 grades of values of alkali, glue denseness 79mm, amylose content 17.4%, all indexs of rice quality all reach three grades of GBs, standard laid down by the ministries or commissions of the Central Government secondary superior rice standard.
C) disease resistance is outstanding, and yield stability is good
2010-2011 carried out disease resistance in continuous 2 years to be identified, salt two excellent 2208 pairs of rice blast, bacterial leaf spot resistance obviously are better than check variety II excellent 838, similar to brown planthopper resistance and check variety II excellent 838, its specific targets are: 5.7 grades of rice blast composite indexes (CK7.2 level), highest 9 grades (the CK9 level) of fringe pest percent of loss; 5 grades of bacterial leaf-blight average levels (CK7 level), highest 5 grades (CK7 level); 8 grades of brown planthopper average levels (CK9 level), highest 9 grades (CK9 level).Rice blast, bacterial leaf-blight and brown planthopper resistance are identified with reference to national new rice variety pilot experiment standard: 0 grade (high anti-), 1 grade (resist), 3 grades (in anti-), 5 grades (middle sense), 7 grades (sense), 9 grades (height is felt).Because salt two excellent 2208 canes are sturdy, leaf sheath is embraced the stem consolidation, has fertilizer resistance anti-lodging performance preferably.
D) economical character is good, wide adaptability
Salt two excellent 2208 seedling are short strong, the short and thick green of bud scale, and blade is straight and upright, and the leaf look dark green, and stooling is stronger.Become the strain plant type collecting and distributing moderate, about plant height 125cm, the total blade 17-18 of stem opens, 5 elongation internodes, and three functional leafs in top are straight and upright, are subjected to the light attitude good, lift to be the straw mulch top the blue or green seed yellow maturity lover of bar when ripe on the sword-like leave.About the percentage of earbearing tiller 65%, general mu is fringe 15-17 ten thousand effectively, and average mu is fringe 15.8 ten thousand effectively, and the fringe type is bigger, spike length 25.4cm, and several about 168.5 of every total grain panicle, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, thousand kernel weight 30 grams.Than contrast II excellent 838 ripe 5 days late, belong to the basic nutrition vegetative form the 139.5 days time of infertility, in ripe in Xian double-linear hybrid rice type.
2, salt two excellent 2208 culture technique main points
According to testing and demonstration result through multiple years, the output structure that salt two excellent 2208 establishing in large scale per mu yields are 650 kilograms is: mu is fringe 16-18 ten thousand effectively, and about every total grain panicle 175 numbers, ripening rate about 90% is about thousand kernel weight 30 grams.High-yield culturing should striven sufficient fringe, attack on the basis of big fringe, improves ripening rate.Namely by cultivating the strong seedling of of the right age many tillers, rational close planting is shortly early sent out early enough seedling and is in time put the field, improves colony quality, accomplishes fluently the high yield basis, and on the basis of guaranteeing suitable spike number, the short strong big fringe of stalk is taken back again aim of high yield thereby reach sufficient fringe, big fringe, high grain.The high-yield culture technique main points that should grasp are:
(1) proper seeding is cultivated strong seedling
According to various places Rice Production custom, rationally arrange sowing time, guarantee safety heading flowering.The every mu of clean seedling plate amount of broadcasting 10-15 kilogram of water-raised seedling, the every mu of clean seedling plate amount of broadcasting 20-25 kilogram of non-irrigated seedling, land for growing field crops sowing quantity 1.0-1.5 kilogram/mu.Machine transplanting of rice seedling 20-25 day in general May is sowed, every mu of land for growing field crops sowing quantity 1.5-2.0 kilogram.The rice seedling bed will use sufficient base manure, and it is fertile early to execute wean, imposes long thick short fetilizer for tillering, send and transfer fertilizer, and carry out rice seedling bed disease pest and weed control, cultivates the strong seedling of of the right age many tillers.
(2) transplant rational close planting in good time
Transplant, rational close planting is the basis of high-yield culturing in good time.At the 30-35 days water-raised seedling length of time rice seedlings grow, the non-irrigated seedling length of time rice seedlings grow was advisable with 20-25 days, general 18-20 of the machine transplanting of rice seedling length of time rice seedlings grow days.In go up the fertility field and plant soon about specification 1.6-1.8 ten thousand cave/mus, every cave 1-2 this, plant the basic seedling 6-8 ten thousand of foot/mu, the field piece that fertility is low will increase suitably that to plant density be 1.8-2.0 ten thousand cave/mus, accomplish when planting shallow slotting, spare and plant, guarantee to plant quality.
(3) science is with fertile, and short seedling is early sent out
Science is with fertile, and it is the key that forms high yield colony that short seedling is early sent out.Salt two excellent 2208 per mu yields are cultivated more than 650 kilograms, general fertilizer requirement is amounted to about 20 kilograms/mu of purity nitrogens, phosphorus, potash fertilizer are used in collocation, phosphorus, potash fertilizer are main as base manure, fertilising should adhere to " preceding short, in steady, back mend ", principle, base manure accounts for about 60%, tillering fertilizer accounts for about 15%, and fringe granulated fertilizer accounts for about 25%.
(4) run water slurry well, coordinate colony
Water starch management should take that shallow water transplants seedlings, very little water is lived, thin moisture tiller, deep water heading flowering, the irrigation method of later stage alternation of wetting and drying.Requirement was planted back about 20 days, sent out foot and waited the fringe seedling, and the field is put in draining in good time, and the control peak seedling is no more than 250,000/mu, becomes fringe 16-17 ten thousand/mu at last, and moistening endowment of later stage is gathered in and cut off the water supply in preceding 5 days, guaranteed the blue or green seed Huang of stalk.
(5) the anti-worm of curing the disease, keeping a full stand of seedings increases fringe
The with medicament seed soaking prevents and treats seed-borne diseases such as bakanae disease and Aplelenchiodes bessegi of rice before broadcasting.Seeding stage attention control small brown rice planthopper, rice thrips, aphid, field period will be carried out the preventing and controlling of damage by disease and insect such as control rice blast, bacterial leaf-blight, banded sclerotial blight, black streak dwarf, snout moth's larva, brown plant-hopper, guarantee that the rice seedling normal growth grows, and keep a full stand of seedings to strive fringe and strive for high yield.
3, salt two excellent 2208 seeding technique main points
(1) rationally arranges to broadcast the poor phase, guarantee that flower synchronization is good
Salt two excellent 2208 is in the production of hybrid seeds of Yancheng, Jiangsu, and the first phase male parent is sowed by the end of April, and the second stage of male parent was than first phase male parent late sowing 10 days.Maternal early June sowing is broadcast poor time difference 35-36 days with the first phase male parent, leaf difference 6.5-7.0 leaf, and the such time difference and leaf are poor, and the general florescence can meet well.
(2) cultivate strong seedling, suitable row ratio is participated basic seedling
3-4 on male parent band is tillered when generally requiring to transplant, and female parent is with 1-2 to tiller.Production of hybrid seeds old farmer, maternal row are than being advisable with 2:12, and two phase male parent duplicate rows are planted with period interval, distance between rows and hills 30 ㎝ * 15 ㎝; Maternal distance between rows and hills 16.7 ㎝ * 13.3 ㎝ stop action row, and every cave 2-3 originally plants the basic seedling of foot.
(3) the seedling frame is put up in fertile water transfer control, coordinates colony
Seed farm rich water is planned strategies for, grasp " preceding heavy, in steady, back mend " principle, base manure is wanted foot, tillering fertilizer is wanted early, promotes this early growth and quick tiller of father and mother, balanced growth.In time put the field behind enough seedlings, the control ineffective tillering; The late growth stage alternation of wetting and drying is irrigated, and supports Gen Baoye, promotes that grouting is solid.
(4) spray " 920 " in right amount, improve outcrossing seed-setting rate
Maternal salt 220S fringe type is big, bag neck degree is lighter, seed farm " 920 " consumption is too much unsuitable, general consumption 30-40 gram/mu divides three uses, for the first time consumption 10-15 gram/mu, see that at the seed farm female parent fringe 5% begins to cut leaf and used the same day, for the second time, consumption is respectively 15-20 gram/mu and 5-10 gram/mu for the third time, " 920 " for the first time with after sprayed in second day and the 4th day, can solve male sterile line bag neck problem preferably, promote that the fringe layer is neat, improve outcrossing seed-setting rate and production of hybrid seeds per mu yield.
(5) grasp the key link period, carry out the anti-assorted pure work of protecting conscientiously
Under the prerequisite of strict time and space isolation, florescence impurity elimination work is extremely important, is the anti-assorted most important thing of protecting pure work.Seed farm from see fringe to cut behind the leaf " 920 " use tell on before, approximately 6-7 days time was the material time of florescence impurity elimination work, at the sturdy impurity elimination 3-4 of this material time time, can play a multiplier effect to improving seed production purity.In addition, during the ripe and harvested, need guarantee that male parent and maternal list are received, list shines, the single storage, this also is that anti-mixing protected one of pure work.
(6) note diseases and pests controlling, improve the seed commodity
During the paddy growth, note the control of damage by disease and insect such as rice thrips, aphid, banded sclerotial blight, snout moth's larva, rice fulgorid, to guarantee that rice growth is normal.Note using the anti-microbial type agricultural chemicals, to the control disease, improve the paddy look, improving the seed commodity has remarkable result, and major measure has three, and one is to use jinggangmeisu, uses twice booting stage, takes place suppressing banded sclerotial blight, and the strong stalk of healthy tree has obvious effect; Two are to use " going out black No. 1 " bactericide, and female parent uses twice after cutting leaf, both can prevent and treat grain smut, can improve the paddy look again; The 3rd, in good time harvesting is accomplished " ninety percent ripe hundred per cent receive " and is in time dried, and is conducive to improve the commodity of germination rate and seed.
4, salt 220S propagation technique main points
Indica type photo-thermo-sensitive genic male sterile line salt 220S breeding: the place is chosen in Sanya, Hainan, is arranged in annual mid or late November sowing time, and in mid-February, second heading obtains comparatively desirable reproductive output easily for best.

Claims (3)

1. the breeding method of a high-grain weight excellent-quality double-linear hybrid rice kind may further comprise the steps:
Be that the indica type photo-thermo-sensitive genic male sterile line salt 220S and two of CGMCC NO. 7558 is that to recover be the extensive 888 preparing hybrid rice combination of salt with preserving number, obtain high-grain weight excellent-quality double-linear hybrid rice new varieties.
2. according to the breeding method of the described a kind of high-grain weight excellent-quality double-linear hybrid rice kind of claim 1, breed high-grain weight excellent-quality double-linear hybrid rice new varieties, name to salt two excellently 2208, it is characterized in that:
Salt two excellent 2208 seedling are short strong, the short and thick green of bud scale, and blade is straight and upright, and the leaf look dark green, and stooling is stronger; Become the strain plant type collecting and distributing moderate, the average 125cm of plant height, the total blade 17-18 of stem opens, 5 elongation internodes, three functional leafs in top are straight and upright, are subjected to the light attitude good, lift to be the straw mulch top the blue or green seed yellow maturity lover of bar when ripe on the sword-like leave; The percentage of earbearing tiller is average 65%, and general mu is fringe 15-17 ten thousand effectively, and average mu is fringe 15.8 ten thousand effectively, and the fringe type is bigger, the average 25.4cm of spike length, and average 168.5 of every total grain panicle number, the real grain of every fringe is several more than 140, and ripening rate is more than 80%, average 30 grams of thousand kernel weight; Average 139.5 days of the time of infertility than contrast II excellent 838 ripe 5 days late, belonged to the basic nutrition vegetative form, in ripe in Xian double-linear hybrid rice type.
3. the producing method for seed of the described high-grain weight excellent-quality double-linear hybrid rice of claim 1 new varieties is characterized in that:
The producing method for seed of salt two excellent 2208 is the sowings of extensive 888 minutes two phases of male parent salt, I phase male parent is sowed by the end of April, II phase male parent was than I phase male parent late sowing 10 days, maternal than I phase male parent late sowing 35-36 days, this line number of seed farm father and mother is than being 2:12, I, single this alternate the planting of II phase male parent, maternal seeding row spacing 13cm * 16.5cm, every cave 2-3 originally plants the basic seedling of foot.
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