CN108077061A - A kind of selection made wine with the glutinous two-line sterile lines of Xian - Google Patents
A kind of selection made wine with the glutinous two-line sterile lines of Xian Download PDFInfo
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- CN108077061A CN108077061A CN201711377659.9A CN201711377659A CN108077061A CN 108077061 A CN108077061 A CN 108077061A CN 201711377659 A CN201711377659 A CN 201711377659A CN 108077061 A CN108077061 A CN 108077061A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selections made wine with the glutinous two-line sterile lines of Xian, belong to Special Rices breeding technical field.The present invention selects indica type glutinous rice maintainer, high yield resistance upland rice variety, thick stalk, rice variety resistant to lodging, storage tolerance rice variety to make recurrent parent for hybrid strain, two-line sterile line, by hybridizing, reestablishing diplomatic relations and pollen cultures and offspring plant screening, high yield, critical temperature of sterility≤23.0 DEG C, drought resisting, wine brewing resistant to lodging, storage endurance the glutinous two-line sterile lines of Xian are selected.The sterile line and corresponding restorer can combo go out the glutinous hybrid paddy rices of wine brewing Xian, peasant planting can increase by 480 yuan of income or more per acre.The method of the present invention is scientific and efficient, easily operated.
Description
Technical field
Present invention relates particularly to a kind of selections made wine with the glutinous two-line sterile lines of Xian, belong to Special Rices breeding technique neck
Domain.
Background technology
It is the glutinous rice wine that primary raw material is brewed with glutinous rice, it is deep with its unique taste to be liked by the people.In rice breeding
In, there is breeder to carry out breed breeding as target using purposes of making wine, as the institute of agricultural sciences of Shaoxin City be suitble to brewing yellow wine with excellent
Matter glutinous rice is the breed breeding of target, is successively bred as continuing glutinous 86, continue the kinds such as glutinous 119.It makes wine mainly based on glutinous rice, vintager
Industry has certain requirement to the rice matter and appearance index of glutinous rice.Coarse Rice Rate and head rice rate influence liquor output rate, amylose content
Number, the size of gel consistence can all affect pol, alcohol taste of wine etc..Research shows to be suitable for brewing high-quality high-grade
The rice matter standard of the glutinous rice variety of rice wine is：Head rice rate will be more than 60%, amylose ＜ 1.5%, gelatinization point (base number)
7.0,100 mm of gel consistence, the grain of rice will be grown.
The present invention selects indica type glutinous rice maintainer, high resistance to cold and diseases upland rice variety, thick stalk, rice variety resistant to lodging, storage tolerance long-grained nonglutinous rice product
Kind make recurrent parent for hybrid strain, two-line sterile line, by hybridizing, reestablishing diplomatic relations and pollen cultures and offspring plant screening, choosing
Bring out high yield, critical temperature of sterility≤23.0 DEG C, drought resisting, wine brewing resistant to lodging, storage endurance the glutinous two-line sterile lines of Xian.The infertility
System with corresponding restorer can combo go out the glutinous hybrid paddy rices of wine brewing Xian, rice is suitable for making wine, wine brewing sugar valency height, solid content
More, distillation yield height.The method of the present invention is scientific and efficient, easily operated, is selection and breeding, is provided using the kind of wine brewing high-quality hybrid glutinous rice
Source provides scientific method and foundation.
The content of the invention
It is an object of the invention to be directed to the deficiency of existing breeding technique, providing one kind can be with combo wine brewing high-quality hybrid
The selection of the glutinous two-line sterile lines of Xian of glutinous rice.
To achieve the above object, the present invention adopts the following technical scheme that.
A kind of selection made wine with the glutinous two-line sterile lines of Xian, comprises the following steps：
（1）Juvenile stage：Indica type glutinous rice maintainer is selected to praise agriculture wx2B as P1, the strong upland rice variety original upland rice No. 3 of high yield resistance
（The selection and breeding of good quality and high output drought-resistant variety original upland rice No. 3, China seed industry, 2014（3）：70-71）For P2, thick stalk, long-grained nonglutinous rice resistant to lodging
Strain Fuhui 838 is P3, and two autumn of storage tolerance rice strain is No. 1 short（The screening of Long-storage Rice Germplasm Resources, Agriculture in Jiangxi
Report, 2008,20 (4):17-19）For P4, using P1, P2, P3 and P4 as hybrid strain, two-line sterile line " Y58S " or " extensively account for
63S " makees recurrent parent S；
（2）Hybridize combo and offspring's plantation, selection method：
1）Work hybridizes：P1×P2→F1-12；P3×P4→F1-34；
2）Plant first generation of hybrid F1-12、F1-34, florescence makees hybridization F1-12/F1-34, a generation of reestablishing diplomatic relations is obtained, with F1-1234It represents；
3）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Children is removed on superclean bench
Fringe, 0.1wt% HgCl2Solution disinfection 10 minutes, sterile water wash 5 times are inoculated in SK3 culture mediums after drying up flower pesticide surface moisture
Upper evoked callus, inoculum concentration are 2400-2600 flower pesticide；Callus subculture, which is placed in N6 culture mediums for 1 time, to be divided
Change induction, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, it is long
Into seedling；It is finally transferred to take root on the MS culture mediums of no hormone, grows up to plantlet；It is transplanted in greenhouse and plants again, removal is single
Times and polyploid, retain liploid plant, it is ripe after single plant sowing obtain DH groups, represented with DH1；
4）DH1 is planted, group 800-900 plants, in the heading maturity period, DH1 groups selection stalk is sturdy, plant type is compact, effectively divides
Tiller number is obtained in 10-12, more than paddy grain length 8.0mm, setting percentage up to the excellent single plant harvest seed of more than 86%, economical character
DH2；
5）In laboratory using miniature rice mill the DH2 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80%, brown rice performance glutinous rice character White-opalescent and no separated single plant；Upper menu strain takes part kind
Son measures head rice rate, and the single-strain seed for selecting head rice rate high carries out high temperature resistant storage properties screening, selects storage endurance single plant；Upper choosing
Single plant rice carries out amylose content and gelatinization point measures, and selects amylose content ＜ 1.2%, gelatinization point (base number)
The single plant of ＜ 7.0, gel consistence 100mm；
6）Light seeding 5 on nonirrigated farmland）Screening gained DH2 single plants, each strain plant group 80-90 plants, have sowed and sprinkled profoundly water, afterwards
It does not rewater, screening of drought resistance is carried out to rice shoot within after planting 25 days, removal tiller is poor, plant is thin and delicate, the seedling of yellow leaf, choosing point
Tiller power is strong, plant strain growth is vigorous, the rice seedling of blade edge, is transplanted to paddy field；Maturity period selection rice straw is sturdy, plant type is compact, effective
Tiller number is in 10-12, more than paddy grain length 8.0mm, setting percentage up to more than 86%, blade annesl is good, time of infertility 115-130
It strain therefrom selects elite seedling harvest seed to obtain DH3；
7）In laboratory using small-sized brown rice machine the DH3 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80%, brown rice performance glutinous rice character White-opalescent and no separated single plant；On single-strain seed is selected to carry out
High temperature resistant storage properties are screened, and select storage endurance single plant；Upper menu strain rice carries out amylose content and gelatinization point measures, choosing
Select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
8）The DH3 single plants that select and long-grained nonglutinous rice two-line sterile line S in strain plantation, florescence selection plant stalk is sturdy, upright blade,
Sword-like leave is short, single-strain tassel number is more, fringe head is big, neat unseparated excellent strain 2-3 of strain performance, therefrom respectively selects elite seedling 2
Strain is listed as male parent, is hybridized by female parent of S, must hybridize for 1 generation with F1-SIt represents；
9）Plant F1-SWith sterile line S, florescence is returned by female parent of S, must be returned for 1 generation with BC1F1-SIt represents；
10）Plant BC1F1-S, sowing obtains BC1F2-S；Using small-sized brown rice machine to the BC of harvest in laboratory1F2-SSeed is added
Work goes out rough, selects the brown rice of the White-opalescent of brown rice appearance glutinous rice character；The BC selected in plantation1F2-SBrown rice is bloomed
Phase selects the thorough single plant 80-100 plants of listing marks of pollen sterility, and the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration,
Florescence observes pollen fertility, and 20-30 single plant sowing of single plant that pollen fertility is good, setting percentage is high is selected to obtain BC1F3-S；
11）Using miniature rice mill to the BC of harvest in laboratory1F3-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80%, the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain kind
Son carries out high temperature resistant storage properties screening, selects storage endurance single plant；On storage endurance single plant rice is selected to carry out amylose content and paste
Change temperature measuring, select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
12）Light seeding 11 on nonirrigated farmland）Screening gained BC1F3-S, florescence is arranged in summer（Daytime temperature is at 28-38 DEG C）Season, often
It a strain group 40-45 plants, has sowed and has sprinkled profoundly water, do not rewatered afterwards, screening of drought resistance had been carried out to rice shoot in after planting 25 days, gone
It except tiller is poor, plant is thin and delicate, the seedling of yellow leaf, selects that tillering ability is strong, plant strain growth is vigorous, the rice seedling of blade edge, is transplanted to water
Field；Florescence investigates fertility, selects the strain 10 of pollen performance infertility, therefrom selects sturdy plant stalk, upright blade, sword-like leave
Each 5 plants of single plants short, that single-strain tassel number is more, fringe head is big take microscope in tassel room to carry out pollen microscopic examination, pollen performance infertility completely
Single plant listing mark；
13）By 12）Listing mark single plant cuts pocket and moves on to temperate-controlled intraocular's weather chamber planting, from the formation stage of pistil and stamen to ear differentiation
The temperature in phase greenhouse is controlled in 20-23.3 DEG C, carries out fertility screening, selects sturdy plant stalk, upright blade, sword-like leave short, single
The fertile single plant that strain spike number is more, fringe head is big, maturity period single plant sowing obtain BC1F4-S；
14）Using miniature rice mill to the BC of harvest in laboratory1F4-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80%, the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain kind
Son carries out high temperature resistant storage properties screening, selects storage endurance single plant；On storage endurance single plant rice is selected to carry out amylose content and paste
Change temperature measuring, select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
15）Strain plantation 14）The BC of upper choosing1F4-S, pollen fertility, infertility in temperate-controlled intraocular's weather greenhouse to selected strain
Conversion temperature is identified, is selected critical temperature of sterility≤23.0 DEG C, the thorough strain breeding seed of sterile phase abortion, that is, is bred as
The wine brewing glutinous two-line sterile lines of Xian.
Beneficial effects of the present invention：
High yield, critical temperature of sterility≤23.0 DEG C, drought resisting, wine resistant to lodging, storage endurance can be selected using the method for the present invention
The wine glutinous two-line sterile lines of Xian；The sterile line and corresponding restorer can combo go out the glutinous hybrid paddy rices of wine brewing Xian, peasant planting is every
Mu can increase by 480 yuan of income or more, and rice is suitable for making wine, and making wine, sugared valency is high, solid content is more, distillation yield is high；This hair
Bright methodological science is efficient, easily operated, for selection and breeding, using wine brewing with the variety source of high-quality hybrid glutinous rice provide scientific method and
Foundation gives full play to China's rice pest insects advantage, and certain impetus is played to the sound development of China's wine brewing industry.
Specific embodiment：
Embodiment 1：
（1）Juvenile stage：Indica type glutinous rice maintainer is selected to praise agriculture wx2B-P1, No. 3-P2 of upland rice variety original upland rice, long-grained nonglutinous rice resistant to lodging
Strain Fuhui 838-P3, two autumn of storage tolerance strain short No. 1-P4 are hybrid strain, and two-line sterile line " Y58S "-S makees samsara parent
This；
（2）Hybridize combo and offspring's plantation, selection method：
1）Work hybridizes：P1×P2→F1-12；P3×P4→F1-34；
2）Plant first generation of hybrid F1-12、F1-34, florescence makees hybridization F1-12/F1-34, a generation of reestablishing diplomatic relations is obtained, with F1-1234It represents；
3）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Children is removed on superclean bench
Fringe, 0.1wt% HgCl2Solution disinfection 10 minutes, sterile water wash 5 times are inoculated in SK3 culture mediums after drying up flower pesticide surface moisture
Upper evoked callus, inoculum concentration are 2400 flower pesticide；Callus subculture, which is placed in N6 culture mediums for 1 time, to carry out differentiation and lures
It leads, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, grow up to small
Seedling；It is finally transferred to take root on the MS culture mediums of no hormone, grows up to plantlet；Be transplanted in greenhouse and plant again, single times of removal and
Polyploid retains liploid plant, and single plant sowing obtains DH groups after maturation, is represented with DH1；
4）DH1 is planted, 800 plants of group, in the heading maturity period, DH1 groups selection stalk is sturdy, plant type is compact, available tillering
In 10-12, more than paddy grain length 8.0mm, setting percentage DH2 is obtained up to the excellent single plant harvest seed of more than 86%, economical character；
5）In laboratory using miniature rice mill the DH2 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80%, brown rice performance glutinous rice character White-opalescent and no separated single plant；Upper menu strain takes part kind
Son measures head rice rate, and the single-strain seed for selecting head rice rate high carries out high temperature resistant storage properties screening, selects storage endurance single plant；Upper choosing
Single plant rice carries out amylose content and gelatinization point measures, and selects amylose content ＜ 1.2%, gelatinization point (base number)
The single plant of ＜ 7.0, gel consistence 100mm；
6）Light seeding 5 on nonirrigated farmland）Screening gained DH2 single plants, each strain plant 80 plants of group, have sowed and sprinkled profoundly water, afterwards not
It rewaters, screening of drought resistance is carried out to rice shoot within after planting 25 days, removal tiller is poor, plant is thin and delicate, the seedling of yellow leaf, selects tiller
Power is strong, plant strain growth is vigorous, the rice seedling of blade edge, is transplanted to paddy field；Maturity period selection rice straw is sturdy, plant type is compact, effectively divides
Tiller number is in 10-12, more than paddy grain length 8.0mm, setting percentage up to more than 86%, blade annesl is good, the 115-130 days time of infertility
Strain, therefrom select elite seedling harvest seed obtain DH3；
7）In laboratory using small-sized brown rice machine the DH3 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80%, brown rice performance glutinous rice character White-opalescent and no separated single plant；On single-strain seed is selected to carry out
High temperature resistant storage properties are screened, and select storage endurance single plant；Upper menu strain rice carries out amylose content and gelatinization point measures, choosing
Select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
8）The DH3 single plants that select and long-grained nonglutinous rice two-line sterile line S in strain plantation, florescence selection plant stalk is sturdy, upright blade,
Sword-like leave is short, single-strain tassel number is more, fringe head is big, the neat unseparated excellent strain 2 of strain performance, therefrom respectively selects 2 plants of elite seedling
It is listed as male parent, is hybridized by female parent of S, must hybridize for 1 generation with F1-SIt represents；
9）Plant F1-SWith sterile line S, florescence is returned by female parent of S, must be returned for 1 generation with BC1F1-SIt represents；
10）Plant BC1F1-S, sowing obtains BC1F2-S；Using small-sized brown rice machine to the BC of harvest in laboratory1F2-SSeed is added
Work goes out rough, selects the brown rice of the White-opalescent of brown rice appearance glutinous rice character；The BC selected in plantation1F2-SBrown rice is bloomed
Phase selects the thorough 80 plants of listing marks of single plant of pollen sterility, and the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, blooms
Phase observes pollen fertility, and 20 single plant sowings of single plant that pollen fertility is good, setting percentage is high is selected to obtain BC1F3-S；
11）Using miniature rice mill to the BC of harvest in laboratory1F3-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80%, the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain kind
Son carries out high temperature resistant storage properties screening, selects storage endurance single plant；On storage endurance single plant rice is selected to carry out amylose content and paste
Change temperature measuring, select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
12）Light seeding 11 on nonirrigated farmland）Screening gained BC1F3-S, florescence is arranged in summer high-temperature season（Daytime temperature is in 28-38
℃）, each 40 plants of strain group has sowed and has sprinkled profoundly water, do not rewatered afterwards, carries out drought resistance sieve to rice shoot within after planting 25 days
Choosing, removal tiller is poor, plant is thin and delicate, the seedling of yellow leaf, selects that tillering ability is strong, plant strain growth is vigorous, the rice seedling of blade edge, transplants
To paddy field；Florescence investigate fertility, select pollen performance infertility strain 10, therefrom select sturdy plant stalk, upright blade,
Each 5 plants of the single plant that sword-like leave is short, single-strain tassel number is more, fringe head is big takes microscope in tassel room to carry out pollen microscopic examination, and pollen performance is complete
The single plant listing mark of infertility；
13）By 12）Listing mark single plant cuts pocket and moves on to temperate-controlled intraocular's weather chamber planting, from the formation stage of pistil and stamen to ear differentiation
The temperature in phase greenhouse is controlled in 20 DEG C, carries out fertility screening, selects that sturdy plant stalk, upright blade, sword-like leave be short, single-strain tassel number
Fertile single plant more, fringe head is big, maturity period single plant sowing obtain BC1F4-S；
14）Using miniature rice mill to the BC of harvest in laboratory1F4-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80%, the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain kind
Son carries out high temperature resistant storage properties screening, selects storage endurance single plant；On storage endurance single plant rice is selected to carry out amylose content and paste
Change temperature measuring, select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
15）Strain plantation 14）The BC of upper choosing1F4-S, pollen fertility, infertility in temperate-controlled intraocular's weather greenhouse to selected strain
Conversion temperature is identified, is selected critical temperature of sterility≤23.0 DEG C, the thorough strain breeding seed of sterile phase abortion, that is, is bred as
The wine brewing glutinous two-line sterile lines of Xian-wine rice -1S.
Wine rice -1S high yields, critical temperature of sterility≤22.8 DEG C, drought resisting, resistant to lodging, storage endurance.Wine rice -1S recovers with glutinous rice
System praises the glutinous hybrid paddy rices of Xian of glutinous extensive No. 2 combos, 670 kilograms of per mu yield, and peasant planting can increase by 490 yuan of income, rice per acre
It is suitable for making wine, making wine, sugared valency is high, solid content is more, distillation yield is high.
Embodiment 2：
（1）Juvenile stage：Indica type glutinous rice maintainer is selected to praise agriculture wx2B-P1, the strong upland rice variety original upland rice No. 3 of high yield resistance-
P2, thick stalk, long-grained nonglutinous rice strain Fuhui 838-P3 resistant to lodging, two autumn of storage tolerance rice strain short No. 1-P4 are hybrid strain, and two systems are not
Educate is that Guangzhan 63S-S makees recurrent parent；
（2）Hybridize combo and offspring's plantation, selection method：
1）Work hybridizes：P1×P2→F1-12；P3×P4→F1-34；
2）Plant first generation of hybrid F1-12、F1-34, florescence makees hybridization F1-12/F1-34, a generation of reestablishing diplomatic relations is obtained, with F1-1234It represents；
3）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Children is removed on superclean bench
Fringe, 0.1wt% HgCl2Solution disinfection 10 minutes, sterile water wash 5 times are inoculated in SK3 culture mediums after drying up flower pesticide surface moisture
Upper evoked callus, inoculum concentration are 2600 flower pesticide；Callus subculture, which is placed in N6 culture mediums for 1 time, to carry out differentiation and lures
It leads, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, grow up to small
Seedling；It is finally transferred to take root on the MS culture mediums of no hormone, grows up to plantlet；Be transplanted in greenhouse and plant again, single times of removal and
Polyploid retains liploid plant, and single plant sowing obtains DH groups after maturation, is represented with DH1；
4）DH1 is planted, 900 plants of group, in the heading maturity period, DH1 groups selection stalk is sturdy, plant type is compact, available tillering
In 10-12, more than paddy grain length 8.0mm, setting percentage DH2 is obtained up to the excellent single plant harvest seed of more than 86%, economical character；
5）In laboratory using miniature rice mill the DH2 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80%, brown rice performance glutinous rice character White-opalescent and no separated single plant；Upper menu strain takes part kind
Son measures head rice rate, and the single-strain seed for selecting head rice rate high carries out high temperature resistant storage properties screening, selects storage endurance single plant；Upper choosing
Single plant rice carries out amylose content and gelatinization point measures, and selects amylose content ＜ 1.2%, gelatinization point (base number)
The single plant of ＜ 7.0, gel consistence 100mm；
6）Light seeding 5 on nonirrigated farmland）Screening gained DH2 single plants, each strain plant group 80-90 plants, have sowed and sprinkled profoundly water, afterwards
It does not rewater, screening of drought resistance is carried out to rice shoot within after planting 25 days, removal tiller is poor, plant is thin and delicate, the seedling of yellow leaf, choosing point
Tiller power is strong, plant strain growth is vigorous, the rice seedling of blade edge, is transplanted to paddy field；Maturity period selection rice straw is sturdy, plant type is compact, effective
Tiller number is in 10-12, more than paddy grain length 8.0mm, setting percentage up to more than 86%, blade annesl is good, time of infertility 115-130
It strain therefrom selects elite seedling harvest seed to obtain DH3；
7）In laboratory using small-sized brown rice machine the DH3 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80%, brown rice performance glutinous rice character White-opalescent and no separated single plant；On single-strain seed is selected to carry out
High temperature resistant storage properties are screened, and select storage endurance single plant；Upper menu strain rice carries out amylose content and gelatinization point measures, choosing
Select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
8）The DH3 single plants that select and long-grained nonglutinous rice two-line sterile line S in strain plantation, florescence selection plant stalk is sturdy, upright blade,
Sword-like leave is short, single-strain tassel number is more, fringe head is big, the neat unseparated excellent strain 3 of strain performance, therefrom respectively selects 2 plants of elite seedling
It is listed as male parent, is hybridized by female parent of S, must hybridize for 1 generation with F1-SIt represents；
9）Plant F1-SWith sterile line S, florescence is returned by female parent of S, must be returned for 1 generation with BC1F1-SIt represents；
10）Plant BC1F1-S, sowing obtains BC1F2-S；Using small-sized brown rice machine to the BC of harvest in laboratory1F2-SSeed is added
Work goes out rough, selects the brown rice of the White-opalescent of brown rice appearance glutinous rice character；The BC selected in plantation1F2-SBrown rice is bloomed
Phase selects the thorough 100 plants of listing marks of single plant of pollen sterility, and the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration, blooms
Phase observes pollen fertility, and 30 single plant sowings of single plant that pollen fertility is good, setting percentage is high is selected to obtain BC1F3-S；
11）Using miniature rice mill to the BC of harvest in laboratory1F3-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80%, the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain kind
Son carries out high temperature resistant storage properties screening, selects storage endurance single plant；On storage endurance single plant rice is selected to carry out amylose content and paste
Change temperature measuring, select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
12）Light seeding 11 on nonirrigated farmland）Screening gained BC1F3-S, florescence is arranged in summer high-temperature season（Daytime temperature is in 28-38
℃）, each 45 plants of strain group has sowed and has sprinkled profoundly water, do not rewatered afterwards, carries out drought resistance sieve to rice shoot within after planting 25 days
Choosing, removal tiller is poor, plant is thin and delicate, the seedling of yellow leaf, selects that tillering ability is strong, plant strain growth is vigorous, the rice seedling of blade edge, transplants
To paddy field；Florescence investigate fertility, select pollen performance infertility strain 10, therefrom select sturdy plant stalk, upright blade,
Each 5 plants of the single plant that sword-like leave is short, single-strain tassel number is more, fringe head is big takes microscope in tassel room to carry out pollen microscopic examination, and pollen performance is complete
The single plant listing mark of infertility；
13）By 12）Listing mark single plant cuts pocket and moves on to temperate-controlled intraocular's weather chamber planting, from the formation stage of pistil and stamen to ear differentiation
The temperature in phase greenhouse is controlled in 23.3 DEG C, carries out fertility screening, selects that sturdy plant stalk, upright blade, sword-like leave be short, single plant fringe
The fertile single plant that number is more, fringe head is big, maturity period single plant sowing obtain BC1F4-S；
14）Using miniature rice mill to the BC of harvest in laboratory1F4-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80%, the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain kind
Son carries out high temperature resistant storage properties screening, selects storage endurance single plant；On storage endurance single plant rice is selected to carry out amylose content and paste
Change temperature measuring, select amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0, the single plant of gel consistence 100mm；
15）Strain plantation 14）The BC of upper choosing1F4-S, pollen fertility, infertility in temperate-controlled intraocular's weather greenhouse to selected strain
Conversion temperature is identified, is selected critical temperature of sterility≤23.0 DEG C, the thorough strain breeding seed of sterile phase abortion, that is, is bred as
The wine brewing glutinous two-line sterile lines of Xian-wine rice -2S.
Wine rice -1S high yields, critical temperature of sterility≤22.6 DEG C, drought resisting, resistant to lodging, storage endurance.Wine rice -2S recovers with glutinous rice
System praises the glutinous hybrid paddy rices of Xian of glutinous extensive No. 2 combos, 682 kilograms of per mu yield, and peasant planting can increase by 485 yuan of income, rice per acre
It is suitable for making wine, making wine, sugared valency is high, solid content is more, distillation yield is high.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (1)
1. a kind of selection made wine with the glutinous two-line sterile lines of Xian, it is characterised in that：The selection comprises the following steps：
（1）Juvenile stage：Indica type glutinous rice maintainer is selected to praise agriculture wx2B as P1, the strong upland rice variety original upland rice No. 3 of high yield resistance
For P2, thick stalk, long-grained nonglutinous rice strain Fuhui 838 resistant to lodging are P3, and short No. 1 of two autumn of storage tolerance rice strain is P4, with P1, P2, P3 and
P4 makees recurrent parent S as hybrid strain, two-line sterile line " Y58S " or " Guangzhan 63S "；
（2）Hybridize combo and offspring's plantation, selection method：
1）Work hybridizes：P1×P2→F1-12；P3×P4→F1-34；
2）Plant first generation of hybrid F1-12And F1-34, florescence makees hybridization F1-12/F1-34, a generation of reestablishing diplomatic relations is obtained, with F1-1234It represents；
3）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Children is removed on superclean bench
Fringe, 0.1wt% HgCl2Solution disinfection 10 minutes, sterile water wash 5 times are inoculated in SK3 cultures after drying up flower pesticide surface moisture
Evoked callus on base, inoculum concentration are 2400-2600 flower pesticide；Callus subculture, which is placed in N6 culture mediums for 1 time, to carry out
The differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate by induction,
Grow up to seedling；It is finally transferred to take root on the MS culture mediums of no hormone, grows up to plantlet；It is transplanted in greenhouse and plants again, remove
Single times and polyploid, retain liploid plant, and single plant sowing obtains DH groups after maturation, is represented with DH1；
4）DH1 is planted, group 800-900 plants, in the heading maturity period, DH1 groups selection stalk is sturdy, plant type is compact, effectively divides
Tiller number is obtained in 10-12, more than paddy grain length 8.0mm, setting percentage up to the excellent single plant harvest seed of more than 86% and economical character
DH2；
5）In laboratory using miniature rice mill the DH2 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80% and the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain takes part kind
Son measures head rice rate, and the single-strain seed for selecting head rice rate high carries out high temperature resistant storage properties screening, selects storage endurance single plant；Upper choosing
Single plant rice carries out amylose content and gelatinization point measures, and selects amylose content ＜ 1.2%, gelatinization point (base number)
The single plant of ＜ 7.0 and gel consistence 100mm；
6）Light seeding 5 on nonirrigated farmland）Screening gained DH2 single plants, each strain plant group 80-90 plants, have sowed and sprinkled profoundly water, afterwards
It does not rewater, screening of drought resistance is carried out to rice shoot within after planting 25 days, removal tiller is poor, plant is thin and delicate and the seedling of yellow leaf, choosing
Tillering ability is strong, plant strain growth is vigorous and the rice seedling of blade edge, is transplanted to paddy field；Maturity period selection rice straw is sturdy, plant type is compact, has
Tiller number is imitated in 10-12, more than paddy grain length 8.0mm, setting percentage up to more than 86%, blade annesl is good and time of infertility 115-
The strain of 130 days therefrom selects elite seedling harvest seed to obtain DH3；
7）In laboratory using small-sized brown rice machine the DH3 single-strain seeds of harvest are processed out it is rough, select brown rice grain length 7.2mm with
Upper, Coarse Rice Rate ＞ 80% and the White-opalescent of brown rice performance glutinous rice character and no separated single plant；On single-strain seed is selected to carry out
High temperature resistant storage properties are screened, and select storage endurance single plant；Upper menu strain rice carries out amylose content and gelatinization point measures, choosing
Select the single plant of amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0 and gel consistence 100mm；
8）The DH3 single plants that select and long-grained nonglutinous rice two-line sterile line S in strain plantation, florescence selection plant stalk is sturdy, upright blade,
Sword-like leave is short, single-strain tassel number is more, fringe head is big and neat unseparated excellent strain 2-3 of strain performance, therefrom respectively selects elite seedling 2
Strain is listed as male parent, is hybridized by female parent of S, must hybridize for 1 generation with F1-SIt represents；
9）Plant F1-SWith sterile line S, florescence is returned by female parent of S, must be returned for 1 generation with BC1F1-SIt represents；
10）Plant BC1F1-S, sowing obtains BC1F2-S；Using small-sized brown rice machine to the BC of harvest in laboratory1F2-SSeed is added
Work goes out rough, selects the brown rice of the White-opalescent of brown rice appearance glutinous rice character；The BC selected in plantation1F2-SBrown rice is bloomed
Phase selects the thorough single plant 80-100 plants of listing marks of pollen sterility, and the growth cabinet that shift-in temperature is 23 DEG C cuts pocket regeneration,
Florescence observes pollen fertility, and 20-30 single plant sowing of single plant that pollen fertility is good and setting percentage is high is selected to obtain BC1F3-S；
11）Using miniature rice mill to the BC of harvest in laboratory1F3-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80% and the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain
Seed carries out high temperature resistant storage properties screening, selects storage endurance single plant；On select storage endurance single plant rice carry out amylose content and
Gelatinization point measures, and selects the single plant of amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0 and gel consistence 100mm；
12）Light seeding 11 on nonirrigated farmland）Screening gained BC1F3-S, florescence is arranged in season in summer, each strain group 40-45 plants, broadcasts
Finish and sprinkle profoundly water, do not rewater afterwards, screening of drought resistance is carried out to rice shoot within after planting 25 days, tiller is poor for removal, plant is thin and delicate and
The seedling of yellow leaf, selects that tillering ability is strong, plant strain growth is vigorous and the rice seedling of blade edge, is transplanted to paddy field；Florescence investigates fertility,
Select the strain 10 of pollen performance infertility, therefrom select sturdy plant stalk, upright blade, sword-like leave be short, single-strain tassel number mostly and fringe
Each 5 plants of big single plant takes microscope in tassel room to carry out pollen microscopic examination, the single plant listing mark of pollen performance infertility completely；
13）By 12）Listing mark single plant cuts pocket and moves on to temperate-controlled intraocular's weather chamber planting, from the formation stage of pistil and stamen to ear differentiation
The temperature in phase greenhouse is controlled in 20-23.3 DEG C, carries out fertility screening, selects sturdy plant stalk, upright blade, sword-like leave short, single
The strain spike number fertile single plant big mostly with fringe head, maturity period single plant sowing obtain BC1F4-S；
14）Using miniature rice mill to the BC of harvest in laboratory1F4-SSingle-strain seed is processed screening, selects brown rice grain length
More than 7.2mm, Coarse Rice Rate ＞ 80% and the White-opalescent of brown rice performance glutinous rice character and no separated single plant；Upper menu strain
Seed carries out high temperature resistant storage properties screening, selects storage endurance single plant；On select storage endurance single plant rice carry out amylose content and
Gelatinization point measures, and selects the single plant of amylose content ＜ 1.2%, gelatinization point (base number) ＜ 7.0 and gel consistence 100mm；
15）Strain plantation 14）The BC of upper choosing1F4-S, to the pollen fertility and infertility of selected strain in temperate-controlled intraocular's weather greenhouse
Conversion temperature is identified, is selected critical temperature of sterility≤23.0 DEG C and the thorough strain breeding seed of sterile phase abortion, that is, is educated
Into the wine brewing glutinous two-line sterile lines of Xian.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113261503A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special indica rice variety for brewing Luzhou-flavor liquor |
| CN113261502A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special rice two-line sterile line for brewing Luzhou-flavor liquor |
| CN113261501A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special glutinous rice variety for brewing Luzhou-flavor liquor |
| CN114766350A (en) * | 2022-05-31 | 2022-07-22 | 绍兴市农业科学研究院 | Breeding method of japonica glutinous rice and application of bred glutinous rice material in yellow rice wine brewing |
-
2017
- 2017-12-19 CN CN201711377659.9A patent/CN108077061A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113261503A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special indica rice variety for brewing Luzhou-flavor liquor |
| CN113261502A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special rice two-line sterile line for brewing Luzhou-flavor liquor |
| CN113261501A (en) * | 2021-05-07 | 2021-08-17 | 湖南杂交水稻研究中心 | Breeding method of special glutinous rice variety for brewing Luzhou-flavor liquor |
| CN114766350A (en) * | 2022-05-31 | 2022-07-22 | 绍兴市农业科学研究院 | Breeding method of japonica glutinous rice and application of bred glutinous rice material in yellow rice wine brewing |
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