CN105580729A - Culture method of cymbidium hybrid orchids - Google Patents
Culture method of cymbidium hybrid orchids Download PDFInfo
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- CN105580729A CN105580729A CN201610053360.7A CN201610053360A CN105580729A CN 105580729 A CN105580729 A CN 105580729A CN 201610053360 A CN201610053360 A CN 201610053360A CN 105580729 A CN105580729 A CN 105580729A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
A culture method of cymbidium hybrid orchids is characterized by comprising steps of greenhouse culture and blooming regulation of parent cymbidium hybrid, hybrid orchids and cymbidium materials: a) culture of a female parent material; b) culture of a male parent material; c) hybrid pollination; d) ovary and seed development; e) sterile sowing and the like. The method is used for culturing good hybrid orchid offspring materials with characteristics of cymbidium; different from existing cymbidium hybrid and other cymbidium sinense hybrid orchids, cymbidium ensifolium hybrid orchids and the like in the aspect of morphological characteristics, growth characteristics, stress resistance and the like, the cymbidium hybrid orchids have excellent properties of the cymbidium, and the variety type of the cymbidium hybrid orchids can be enriched.
Description
Technical field
The invention belongs to field of plant breeding, be specifically related to a kind of method for creating of Cymbidium hybrid orchid.
Background technology
The hybridization that hybrid cymbidium (CymbidiumHybrid) is grown nonparasitically upon another plant kind for part great Hua type in the orchid family epidendrumKind, the crossbreeding history of existing more than 100 year of hybrid cymbidium, their parent is mainly great Hua typeTorrid zone epiphytic orchid class, usually will pass through repetitious hybridization, could form valuable kind, is the worldOne of cattleya that upper sales volume is the highest. Although hybrid cymbidium pattern is bright-coloured, the florescence is long, sight is high, is deeply subject toWorld orchid fan's welcome, but due to the many British plain spirits of hybrid cymbidium, therefore its fancy points still can changeEnter. And the orchid family epidendrum abundant species approximately has the remaining kind of 68-70. Grow nonparasitically upon another plant kind except the torrid zone, also have largeGround, the temperate zone non-hibernating eggs of amount, as the Chunlan in traditional state orchid, orchid, sword-leaved cymbidium, Chinese cymbidium, lotus lobe orchid, cold blueDeng, have at present the materials such as the raw orchid in ground, part temperate zone and hybrid cymbidium to hybridize, cultivate sight between great HuaThe hybrid orchid of the blue intermediate form of orchid and state, has pattern bright-coloured, the features such as tool fragrance.
But current cultivated hybrid orchid kind mostly is the product of the rear seed selection of the hybridization such as hybrid cymbidium and sword-leaved cymbidium and Chinese cymbidiumKind, and important kind in the orchid raw orchid that is temperate zone has good sight, aromatic flavour, and byIn with the kind florescence Time Inconsistencies such as hybrid cymbidium, there is no at present the breed of variety of orchid hybrid orchid and go out.
Summary of the invention:
The object of this invention is to provide a kind of method for creating of Cymbidium hybrid orchid, adopt proper technology control great HuaOrchid blooms and hybridizes with orchid, cultivates novel hybrid orchid kind, can enrich the kind of Cymbidium hybrid orchidType, the sight of raising hybrid orchid.
Technical scheme of the present invention is as follows:
A creation method for novel Cymbidium hybrid orchid, comprises parent's hybrid cymbidium, hybrid orchid and orchid materialThe step of the hot-house culture of material and the regulation and control of blooming:
A) cultivation of maternal material: taking hybrid cymbidium and hybrid orchid kind as maternal, the maturation of growth 4-5Plant is planted in greenhouse, and winter-spring season control is nourished and grown, and controls N, P, K trophic level, reducesNitrogen, improves phosphorus potash fertilizer concentration, and suitably control temperature early stage, and 3-4 month is improved temperature, controls it in the springJi Kaihua;
B) cultivation of male parent material: select perennial orchid for maternal, by N, P, K nutrition and lightAccording to temperature control, promote orchid bloom in 3-4 month;
C) hybridization pollination: separate massula with tweezers from male parent orchid Flowering Plants, maternal hybrid cymbidium or assortedHand over list on blue Flowering Plants the flowers are in blossom after putting and remove massula, award the massula with male parent orchid, every flower award withOrchid massula 4-6, to guarantee pollination;
D) ovary and seed development: the hybrid cymbidium after pollination is petal wilting after one week, and two Zhou Houneng observeOvary is obviously expanded, and cuts off withered petal and sepal, prevents growing of bacterium and fungi; Growth 210-270After it, the fruit pod of can gathering before fruit pod flavescence cracking.
After the step of the hot-house culture of described parent's hybrid cymbidium, hybrid orchid and orchid material and the regulation and control of bloomingAlso comprise the step of filial generation aseptically sowing seeds:
E) axenic germination culture medium: add 6-BA0.5-2.0 taking 1/2MS or MS as minimal mediummg·L-1And NAA0.5-2.0mgL-1；
F) aseptic seeding and sprouting: the mellow fruit pod of gathering, running water is shown at superclean bench after rinsingFace sterilization treatment, first uses 75% Ethanol Treatment 1 minute, then processes 30-40 with 1% clorox and divideClock, aseptic water washing 3-4 time cuts fruit pod on aseptic filter paper, takes out seed, is seeded in to sprout and cultivatesBase;
G) filial generation plant regeneration: add NAA0.5-1.0mgL taking MS as minimal medium-1With1.0-2.0mg·L-1Suitable protocorm is grown and plant regeneration;
H) plantlet of transplant of taking root: the plant that takes root is opened bottleneck domestication 3-5d, cleans after culture medium with 800-1000Bravo is doubly processed 1-3h, and adopting sphagna is that matrix is transplanted in 2.5 cun of nutritive cubes, after transplanting, controls greenhouseTemperature is at 25 DEG C, and relative air humidity grows new root for 80%, 20 day later and survives, and grows and plants after 6 monthsStrain is further transplanted to larger nutritive cube further growth.
Described step e), step g) and the culture medium of step in h) in add respectively 3% sucrose and 100-150ml·L-1Coconut milk, active carbon 1-2%, pH value is adjusted to 5.4-5.6, and condition of culture is illumination1000lx, 16h photoperiod, 25 DEG C of cultivations.
The invention has the beneficial effects as follows:
Because the seed development of hybrid cymbidium and other kind of material filial generation formation of Cymbidium is incomplete, without embryoBreast, under regular situation, extremely difficult sprouting, need to adopt aseptic seeding technology to sprout, and between planting, edge far away is assorted in additionA little less than handing over offspring's viability, thereby offspring sprouts and breeding difficulty, and aseptic seeding technology can be passed through culture mediumIn nutritional labeling and plant growth regulator promote filial generation seed development and maturation germination. Germinated plantletAfter further growth and seedling, elite plant strain be can in progeny population, filter out, amount reproduction and popularization carried outApplication.
The inventive method is taking the orchid in cymbidium edaphic orchids as male parent, by with maternal hybrid cymbidium or hybridizationBlue hybridization, filial generation aseptically sowing seeds, after Germination And Seedling, in progeny population, filter out have orchid andThe novel Cymbidium hybrid orchid of hybrid cymbidium feature, orchid hybrid orchid can be by the thick flavor of orchid, strong stress resistanceTo transfer to pattern bright-coloured etc. proterties, in the hybrid cymbidium of fast growth and other hybrid orchids.
The inventive method can be for cultivating the good hybrid orchid progeny material with orchid feature, with existing largeFlower orchid and other Chinese cymbidium hybrid orchids and sword-leaved cymbidium hybrid orchid etc. are in the side such as morphological feature and growth characteristics and resistanceFace difference, possesses the merit of orchid, can enrich the variety type of Cymbidium hybrid orchid.
Detailed description of the invention
The invention will be further described below:
Embodiment 1:
1. taking hybrid orchid ' Korea S's peach blossom ' as maternal, orchid is that male parent is hybridized, and filial generation is asepticSowing;
A. the cultivation of maternal material: it is maternal selecting hybrid orchid kind ' Korea S's peach blossom ', growth 4-5'sRipe plant is planted in greenhouse, and winter-spring season control is nourished and grown, and adopts low nitrogen, and spending of high phosphorus, potassium is manyMany No. 2 nutrient solutions water weekly 1 time, and suitably control temperature early stage, and the later stage is improved temperature, impels its 3-4Month blooms;
B. the cultivation of male parent material: select perennial orchid for maternal, by N, P, K nutrition and lightAccording to temperature control, promote orchid bloom in 3-4 month;
C. hybridization pollination: separate pollen with tweezers fast from male parent orchid Flowering Plants, the blue Flowering Plants of hybridization of female parentOn list the flowers are in blossom removes massula after putting, award the massula with male parent orchid, every flower is awarded with orchid massula4-6, to guarantee pollination;
D. ovary and seed development: the hybrid orchid after pollination is petal wilting after 1 week, and 2 Zhou Houneng observe sonRoom obviously expands, and cuts off withered petal and sepal, prevents growing of bacterium and fungi; Grow after 240 days,Fruit pod maturation, the fruit pod of gathering before flavescence cracking.
2 filial generation aseptically sowing seeds:
A. axenic germination culture medium: taking MS as minimal medium, add 6-BA0.5mgL-1WithNAA2.0mg·L-1；
B. aseptic seeding and sprouting: the mellow fruit pod of gathering, running water is shown at superclean bench after rinsingFace sterilization treatment, first uses 75% Ethanol Treatment 1 minute, then processes 30 points with 1% cloroxClock, aseptic water washing 4 times cuts fruit pod on aseptic filter paper, takes out seed, is seeded in to sprout and cultivatesBase; Sowing filial generation seed after 60 days sprouts successively;
C. filial generation plant regeneration: protocorm and the root-like stock sprouted to 5.0mm left and right are inoculated in to MSCulture medium adds NAA0.5mgL-1And 6-BA1.0mgL-1Culture medium, promote protocorm grow andPlant regeneration, protocorm Germination And Seedling successively after 30-60 days, and there is adventitious root;
D. the plantlet of transplant of taking root: the plant that takes root is opened bottleneck domestication 3-5d, cleans after culture medium with 1000 timesBravo process 2h, be colonizated in 2.5 cun of nutritive cubes taking sphagna as matrix, transplant after control greenhouse temperature exist25 DEG C, relative air humidity grows new root for 80%, 20 day later and survives, and the plant after 6 months that grows enters oneStep is transplanted to 3 cun of nutritive cube further growths, and the plant that obtains all shows as epiphytic orchid and temperate zone and give birth to orchidFeature, can further screen elite plant strain and be promoted as new material.
Above condition used medium, except indicating, all adds 3% sucrose and 100-150mlL-1CoconutJuice, active carbon 1%, pH value is adjusted to 5.6, and condition of culture is illumination 1000lx, 16h photoperiod, 25 DEG C of trainingsSupport.
Embodiment recited above is described the preferred embodiment of the present invention, not to thisBright design and scope limit, and are not departing under design concept prerequisite of the present invention unskilled labor in this areaVarious modification and improvement that journey technical staff makes technical scheme of the present invention, all should fall into guarantor of the present inventionProtect scope, the technology contents of request protection of the present invention has all been documented in claims.
Claims (3)
1. a creation method for Cymbidium hybrid orchid, it is characterized in that comprising parent's hybrid cymbidium, hybrid orchid andThe step of the hot-house culture of orchid material and the regulation and control of blooming:
A) cultivation of maternal material: taking hybrid cymbidium and hybrid orchid kind as maternal, the maturation of growth 4-5Plant is planted in greenhouse, and winter-spring season control is nourished and grown, and controls N, P, K trophic level, reducesNitrogen, improves phosphorus potash fertilizer concentration, and suitably control temperature early stage, and 3-4 month is improved temperature, promotes it in the springJi Kaihua;
B) cultivation of male parent material: select perennial orchid for maternal, by N, P, K nutrition and lightAccording to temperature control, promote orchid bloom in 3-4 month;
C) hybridization pollination: separate massula with tweezers from male parent orchid Flowering Plants, maternal hybrid cymbidium or assortedHand over list on blue Flowering Plants the flowers are in blossom after putting and remove massula, award the massula with male parent orchid, every flower award withOrchid massula 4-6, to guarantee pollination;
D) ovary and seed development: the hybrid cymbidium after pollination or hybrid orchid be petal wilting after one week, after two weeksCan observe ovary and obviously expand, cut off withered petal and sepal, prevent growing of bacterium and fungi; GrowthAfter 210-270 days, the fruit pod of can gathering before fruit pod flavescence cracking.
2. according to the method for creating of Cymbidium hybrid orchid described in claims 1, it is characterized in that described parent is largeAfter the step of the hot-house culture of flower orchid, hybrid orchid and orchid material and the regulation and control of blooming, also comprise filial generationThe step of aseptically sowing seeds:
E) axenic germination culture medium: add 6-BA0.5-2.0 taking 1/2MS or MS as minimal mediummg·L-1And NAA0.5-2.0mgL-1；
F) aseptic seeding and sprouting: the mellow fruit pod of gathering, running water is shown at superclean bench after rinsingFace sterilization treatment, first uses 75% Ethanol Treatment 1 minute, then processes 30-40 with 1% clorox and divideClock, aseptic water washing 3-4 time cuts fruit pod on aseptic filter paper, takes out seed, is seeded in to sprout and cultivatesBase;
G) filial generation plant regeneration: add NAA0.5-1.0mgL taking MS as minimal medium-1With1.0-2.0mg·L-1Suitable protocorm is grown and plant regeneration;
H) plantlet of transplant of taking root: the plant that takes root is opened bottleneck domestication 3-5d, cleans after culture medium with 800-1000Bravo is doubly processed 1-3h, and adopting sphagna is that matrix is transplanted in 2.5 cun of nutritive cubes, after transplanting, controls greenhouseTemperature is at 25 DEG C, and relative air humidity 80%, grows new root in 20-30 days later and survive, and grows after 6 monthsPlant is further transplanted to larger nutritive cube further growth.
3. according to the method for creating of Cymbidium hybrid orchid described in claims 2, it is characterized in that described stepE), step g) and the culture medium of step in h) in add respectively 3% sucrose and 100-150mlL-1Coconut palmSub-juice, active carbon 1-2%, pH value is adjusted to 5.4-5.6, and condition of culture is illumination 1000lx, 16h light weekPhase, 25 DEG C of cultivations.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258377A (en) * | 2018-11-14 | 2019-01-25 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | A kind of method of bean cotyledon orchid species matter initiative |
| CN109984030A (en) * | 2019-04-18 | 2019-07-09 | 福建农林大学 | A kind of Cymbidium lianpan unisexuality kind vitro Regeneration System is established and rapid propagation method |
| CN111758561A (en) * | 2020-07-24 | 2020-10-13 | 广东省农业科学院环境园艺研究所 | Sterile sowing and breeding method of hybrid orchid seeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258377A (en) * | 2018-11-14 | 2019-01-25 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | A kind of method of bean cotyledon orchid species matter initiative |
| CN109984030A (en) * | 2019-04-18 | 2019-07-09 | 福建农林大学 | A kind of Cymbidium lianpan unisexuality kind vitro Regeneration System is established and rapid propagation method |
| CN109984030B (en) * | 2019-04-18 | 2022-06-17 | 福建农林大学 | Establishment and rapid propagation method of in vitro regeneration system of cymbidium tortisepalum unisexual variety |
| CN111758561A (en) * | 2020-07-24 | 2020-10-13 | 广东省农业科学院环境园艺研究所 | Sterile sowing and breeding method of hybrid orchid seeds |
| CN111758561B (en) * | 2020-07-24 | 2022-04-15 | 广东省农业科学院环境园艺研究所 | Sterile sowing and breeding method of hybrid orchid seeds |
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