CN101485288A - Method for breeding anti-dry heat-resisting Cymbidium hybridum - Google Patents
Method for breeding anti-dry heat-resisting Cymbidium hybridum Download PDFInfo
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- CN101485288A CN101485288A CNA2009100680042A CN200910068004A CN101485288A CN 101485288 A CN101485288 A CN 101485288A CN A2009100680042 A CNA2009100680042 A CN A2009100680042A CN 200910068004 A CN200910068004 A CN 200910068004A CN 101485288 A CN101485288 A CN 101485288A
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Abstract
The invention relates to a seed selection method for drought and heat resistant cymbidium hybridum. The method comprises the following steps: (1) carrying out the screening and parent selection on seed sources according to the standard of drought and heat resistance; (2) hybridizing the parents which have the drought and heat resistant characteristic and are selected in the step (1); (3) carrying out the seed asepsis culture; (4) carrying out the filial generation culture; and (5) carrying out the breed screening of the filial generation according to the standard of drought and heat resistance so as to complete the seed selection. The seed selection method for drought and heat resistant cymbidium hybridum can make full use of cymbidium hybridum resources at both home and abroad to culture a new cymbidium hybridum species with the proprietary intellectual property rights. The new cymbidium hybridum has the advantages of draught resistance, high temperature resistance, and adaptation to be implanted in North China.
Description
Technical field
The present invention relates to technical field of flower breeding, especially a kind of method for breeding anti-dry heat-resisting Cymbidium hybridum.
Background technology
Hybrid cymbidium is the Da Hua kind of growing nonparasitically upon another plant in the orchid family Cymbidium, and it is formed by the hybridization of Da Hua class original seed usually.Characteristics such as hybrid cymbidium owing to have spends that number is many, lovely luster and plant are tall and big are subjected to liking of flowers fan deeply.Current, China mainly also rests on culture technique, flower forcing technology and the quick propagating technology, and hybrid cymbidium rearing new variety technology is also lacked further investigation the research of hybrid cymbidium.Hybrid cymbidium originates in China west and south, the happiness warm and weather of feeling nice and cool summer in winter, and happiness high humidity high light, the growth thermophilic is 10 ℃ to 25 ℃.Nocturnal temperature is advisable with about 10 ℃, especially flowering stage with temperature maintenance can kind of blooming period prolonging to 4 more than 5 ℃, below 15 ℃ to 5 months.Northern China is dry and make a clear distinction between the four seasons, summer is very hot and winter is cold unusually, because the hybrid cymbidium happiness is warm, moistening and half cloudy environment, therefore its north can occur can not normal growth, bloom less, phenomenon such as the florescence is short, can not satisfy vast northern flowers fan's demand.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of hybrid cymbidium rearing new variety new method is provided.The hybrid cymbidium new varieties of cultivating with this method have anti-do heat-resisting, can normal growth and advantage such as bloom.
The present invention solves its technical problem and is achieved through the following technical solutions:
A kind of method for breeding anti-dry heat-resisting Cymbidium hybridum is characterized in that: its selection may further comprise the steps:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects;
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized;
(3), seed asepsis is cultivated;
(4), filial generation is cultivated;
(5), screening varieties is carried out in filial generation, promptly finish seed selection according to anti-dried heat-resistant standard.
And the anti-standard of doing in the described step (1) is: relative air humidity is not higher than at 20% o'clock, and the normal growth duration was not less than 45 days; Heat-resistant standard is: when temperature was 35 ℃, the normal growth duration was not less than 45 days; When temperature be higher than 35 ℃, when being no more than 41 ℃, the normal growth duration was not less than 45 days.
And the hybridization that the parent is carried out in the step (2) is quadrature or reciprocal cross, and the parent after the artificial supplementary pollination soon carries out quadrature or reciprocal cross, and environmental temperature is not less than 18 ℃, and relative moisture is not less than 15%.
And, after artificial supplementary pollination is finished in the step (2), when capsule presents little Huang, can cut and carry out aseptic seeding.
And the concrete steps of described step (3) are: the alcohol of use 70% is cleaned the capsule surface, cuts under gnotobasis, takes out seed; With 0.1% mercuric chloride sterilization 20～25 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate, condition of culture: 20～26 ℃ of temperature, humidity is no more than 70%, intensity of illumination 7000LUX, medium: MS+6-BA 1.0+IBA 0.1.
And described step (4) comprising: divide bottle, constantly divide a bottle subculture with plant strain growth; Transplant, when height of seedling pushes up bottle cap and is 8～12cm in the blake bottle, carry out acclimatization and transplants; Hardening, it is half-open to cultivate bottle cap, cultivates under 1000LUX illumination 5～7 days, throws off bottle cap, and hardening is 2～3 days again; Transplant; Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance; Matrix: 800 times of liquid of pine bark+tpn, under 4000Lux illumination, cultivated in preceding 15～20 days, increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
Advantage of the present invention and beneficial effect are:
1. the hybrid cymbidium new varieties that adopt the method for the invention to cultivate can resist winter and spring the air drying, can also resist summer high temperature, than existing kind winter-spring season air drought and summer high temperature had stronger patience, the natural weather condition that can adapt to the North China, can normal growth, bloom and the florescence long.
2. the new varieties plant of cultivating according to the method applied in the present invention is tall and big, flower is big, pattern is bright-coloured, red kind symbol happy and lucky, and yellow kind symbol is noble elegant, is particularly suitable for China's market comsupton in Spring Festival custom.
3. when breeding method of the present invention is selected hybrid strain, conscientiously investigate parent's affiliation, count from this generation of parent, on shifted for 6 generations onto, thereby avoided the parent select to slip up some adverse effects that may bring, as the regeneration of the transmission of inbreeding depression, serious plant disease, bad proterties and reduction of whole resistance against diseases or the like, guaranteed that effectively the kind of new hybrid cymbidium is good.
4. culture medium prescription germination rate height, the differentiation rate height that the aseptic seeding step is adopted among the present invention, the differentiation rate of transferring for the second time can reach 4～5 times, releases souls from purgatory that to contain aquosity seedling incidence low, only is 2%, therefore can greatly improve the cultivation survival rate of hybrid cymbidium.
5. the present invention is a kind of method for breeding anti-dry heat-resisting Cymbidium hybridum, and utilization the method for the invention can make full use of domestic and international hybrid cymbidium resource, cultivates the hybrid cymbidium new varieties with independent intellectual property right.This new hybrid cymbidium has resist drying, high temperature resistant, suits in the advantage of northern China plantation.
Description of drawings
Fig. 1 is a cultivation flow chart of the present invention.
Embodiment
The invention will be further described below by specific embodiment, and following examples are descriptive, is not determinate, can not limit protection scope of the present invention with this.
A kind of method for breeding anti-dry heat-resisting Cymbidium hybridum, its selection may further comprise the steps:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects
Collect provenance, the provenance of collecting is screened according to anti-dried heat-resistant standard, select the parent.
Anti-doing: be meant anti-air drying, promptly relative air humidity is lower than at 20% o'clock, and the normal growth duration was not less than 45 days.
Heat-resisting: mainly be meant the ability of resisting high temperature summer, promptly when temperature was 35 ℃, the normal growth duration was not less than 45 days (the intermittent processing); When temperature be higher than 35 ℃, when being no more than 41 ℃ (intermittent handle), the normal growth duration was not less than 45 days.
Also to consider 5 pairs of proterties in addition, promptly pattern, spend size, plant height, length of blade and soccer fraud caulome long-pending.Pattern, preferential white, the red and yellow kind selected.Flower diameter is greater than 12 centimetres, and plant height is greater than 1.0 meters.Upright blade or near upright, the soccer fraud belemnoid goes out obviously, and volume is big.
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized
The parent who selects is carried out quadrature or reciprocal cross, and in the hope of the acquisition seed, and the check parent is as male parent or maternal characteristic.The flower that selection was bursted forth 10～15 days is chosen the flower cap with the toothpick of fining away, and pollinium is chosen, and sticks on the muciparous column cap in stamen column top.Hang up label, indicate time and parent's title, put in the greenhouse and cultivate.Control the temp. and humidity environment in greenhouse well, guarantee parent's normal growth.18 ℃～27 ℃ of temperature, relative moisture is not less than 20%.
(3), seed asepsis is cultivated
After the artificial supplementary pollination, note observing the ovary situation of change, when capsule presents little Huang, can cut and carry out aseptic seeding.
Alcohol with 70% is cleaned the capsule surface, and (superclean bench) cuts under gnotobasis, takes out seed.With 0.1% mercuric chloride sterilization 20～25 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate.
Condition of culture: 20～26 ℃ of temperature, humidity is no more than 70%, intensity of illumination 7000LUX.Medium: MS+6-BA 1.0+IBA 0.1.
(4), filial generation is cultivated
Divide bottle: seed turns green, during the high 2～3cm of bud, promptly carries out the branch bottle on the little protocorm.Every bottle is stayed 5～8 of plantlets.Constantly divide a bottle subculture with plant strain growth.
Transplant: when height of seedling pushes up bottle cap in the blake bottle (8～12cm), carry out acclimatization and transplants.Hardening: it is half-open to cultivate bottle cap, puts than (1000LUX) under the intense light irradiation and cultivates 5～7 days.Throw off bottle cap, hardening is 2～3 days again, transplants.Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance.Matrix: 800 times of liquid of pine bark+tpn.Put in preceding 15～20 days under the weak light (4000Lux) and cultivate.Increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
(5), according to anti-dried heat-resistant standard screening varieties is carried out in filial generation.
Periodic observation progeny plant growing state, record and evaluation filial generation performance.According to anti-dried heat-resistant standard, emphasis observation plant is selected standard compliant kind at last to the resistance and the adaptability of temperature, humidity.
Embodiment 1:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects
Collect provenance, the provenance of collecting is screened according to anti-dried heat-resistant standard, select the parent.Select the parent to be: favourite Cym.Ly510 Enzan Moon ' Ai Nair ' and crescent Cym.Lovely moon crescent.
Anti-doing: be meant anti-air drying, promptly relative air humidity is not higher than at 20% o'clock, and the normal growth duration was not less than 45 days.
Heat-resisting: mainly be meant the ability of resisting high temperature summer, promptly when temperature was 35 ℃, the normal growth duration was not less than 45 days (the intermittent processing); When temperature be higher than 35 ℃, when being no more than 41 ℃ (intermittent handle), the normal growth duration was not less than 45 days.
Also to consider 5 pairs of proterties in addition, promptly pattern, spend size, plant height, length of blade and soccer fraud caulome long-pending.Pattern, preferential white, the red and yellow kind selected.Flower diameter is greater than 12 centimetres, and plant height is greater than 1.0 meters.Upright blade or near upright, the soccer fraud belemnoid goes out obviously, and volume is big.
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized
The parent who selects is carried out quadrature, in the hope of obtaining seed.
Quadrature:
Favourite Cym.Ly510 Enzan Moon ' Ai Nair ' * crescent Cym.Lovely mooncrescent
The flower that selection was bursted forth 10 days is chosen the flower cap with the toothpick of fining away, and pollinium is chosen, and sticks on the muciparous column cap in stamen column top.Hang up label, indicate time and parent's title, put in the greenhouse and cultivate.Control the temp. and humidity environment in greenhouse well, guarantee parent's normal growth.Temperature is 18 ℃, and relative moisture is not less than 20%.
(3), seed asepsis is cultivated
After the artificial supplementary pollination, note observing the ovary situation of change, when capsule presents little Huang, can cut and carry out aseptic seeding.
Alcohol with 70% is cleaned the capsule surface, and (superclean bench) cuts under gnotobasis, takes out seed.With 0.1% mercuric chloride sterilization 20 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate.
Condition of culture: 20 ℃ of temperature, humidity is no more than 70%, intensity of illumination 7000LUX.Medium: MS+6-BA 1.0+IBA 0.1.
(4), filial generation is cultivated
Divide bottle: seed turns green, during the high 2cm of bud, promptly carries out the branch bottle on the little protocorm.Every bottle is stayed 5 of plantlets.Constantly divide a bottle subculture with plant strain growth.
Transplant: when height of seedling pushes up bottle cap in the blake bottle (8cm), carry out acclimatization and transplants.Hardening: it is half-open to cultivate bottle cap, puts than (10000LUX) under the intense light irradiation and cultivates 5 days.Throw off bottle cap, hardening is 2 days again, transplants.Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance.Matrix: 800 times of liquid of pine bark+tpn.Put in preceding 15～20 days under the weak light (4000Lux) and cultivate.Increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
(5), according to anti-dried heat-resistant standard screening varieties is carried out in filial generation.
Periodic observation progeny plant growing state, record and evaluation filial generation performance.According to anti-dried heat-resistant standard, emphasis observation plant is selected standard compliant kind at last to the resistance and the adaptability of temperature, humidity.
The experimental data form:
| Relative moisture | Air themperature | Quantity | Growth time | Survival rate |
| 15％ | 35℃～41℃ | 30 | 45 days | 100％ |
| 5％ | 35℃～41℃ | 30 | 45 days | 91％ |
| 15％ | 35℃～41℃ | 30 | 90 days | 45％ |
Annotate: air themperature is an alternate treatment, and be 35 ℃～41 ℃ up at 3 o'clock in afternoon the morning 10, and be 20 ℃～25 ℃ up to 10 o'clock the next mornings afternoon 3.Air humidity also is an alternate treatment, and be 15% or 5% up at 3 o'clock in afternoon the morning 10, and be 60%～70% up to 10 o'clock the next mornings afternoon 3.
Embodiment 2:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects
Collect provenance, the provenance of collecting is screened according to anti-dried heat-resistant standard, select the parent.Select the parent to be: favourite Cym.Ly510Enzan Moon ' Ai Nair ' and crescent Cym.Lovely moon crescent.
Anti-doing: be meant anti-air drying, promptly relative air humidity is not higher than at 15% o'clock, and the normal growth duration was not less than 45 days.
Heat-resisting: mainly be meant the ability of resisting high temperature summer, promptly when temperature was 35 ℃, the normal growth duration was not less than 45 days (the intermittent processing); When temperature be higher than 35 ℃, when being no more than 41 ℃ (intermittent handle), the normal growth duration was not less than 45 days.
Also to consider 5 pairs of proterties in addition, promptly pattern, spend size, plant height, length of blade and soccer fraud caulome long-pending.Pattern, preferential white, the red and yellow kind selected.Flower diameter is greater than 12 centimetres, and plant height is greater than 1.0 meters.Upright blade or near upright, the soccer fraud belemnoid goes out obviously, and volume is big.
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized
The parent who selects is carried out reciprocal cross, in the hope of obtaining seed.
Reciprocal cross:
Crescent Cym.Lovely moon crescent * favourite Cym.Ly510Enzan Moon ' Ai Nair '
The flower that selection was bursted forth 15 days is chosen the flower cap with the toothpick of fining away, and pollinium is chosen, and sticks on the muciparous column cap in stamen column top.Hang up label, indicate time and parent's title, put in the greenhouse and cultivate.Control the temp. and humidity environment in greenhouse well, guarantee parent's normal growth.Temperature is 27 ℃, and relative moisture is not less than 20%.
(3), seed asepsis is cultivated
After the artificial supplementary pollination, note observing the ovary situation of change, when capsule presents little Huang, can cut and carry out aseptic seeding.
Alcohol with 70% is cleaned the capsule surface, and (superclean bench) cuts under gnotobasis, takes out seed.With 0.1% mercuric chloride sterilization 25 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate.
Condition of culture: temperature is 26 ℃, and humidity is no more than 70%, intensity of illumination 7000LUX.Medium: MS+6-BA 1.0+IBA 0.1.
(4), filial generation is cultivated
Divide bottle: seed turns green, during the high 3cm of bud, promptly carries out the branch bottle on the little protocorm.Every bottle is stayed 8 of plantlets.Constantly divide a bottle subculture with plant strain growth.
Transplant: when height of seedling pushes up bottle cap in the blake bottle (12cm), carry out acclimatization and transplants.Hardening: it is half-open to cultivate bottle cap, puts than (10000LUX) under the intense light irradiation and cultivates 7 days.Throw off bottle cap, hardening is 3 days again, transplants.Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance.Matrix: 800 times of liquid of pine bark+tpn.Put in preceding 15 days under the weak light (4000Lux) and cultivate.Increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
(5), according to anti-dried heat-resistant standard screening varieties is carried out in filial generation.
Periodic observation progeny plant growing state, record and evaluation filial generation performance.According to anti-dried heat-resistant standard, emphasis observation plant is selected standard compliant kind at last to the resistance and the adaptability of temperature, humidity.
The experimental data form:
| Relative moisture | Air themperature | Quantity | Growth time | Survival rate |
| 20％ | 35℃～41℃C | 30 | 90 days | 100％ |
| 15％ | 35℃～41℃ | 30 | 45 days | 89％ |
| 5％ | 35℃～41℃ | 30 | 90 days | 45％ |
Embodiment 3:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects
Collect provenance, the provenance of collecting is screened according to anti-dried heat-resistant standard, select the parent.Select the parent to be: sweet moonlight Cym.Organdy ' Moonlight ' and crescent Cym.Lovelymoon crescent
Anti-doing: be meant anti-air drying, promptly relative air humidity is not higher than at 15% o'clock, and the normal growth duration was not less than 45 days.
Heat-resisting: mainly be meant the ability of resisting high temperature summer, promptly when temperature was 35 ℃, the normal growth duration was not less than 45 days (the intermittent processing); When temperature be higher than 35 ℃, when being no more than 41 ℃ (intermittent handle), the normal growth duration was not less than 45 days.
Also to consider 5 pairs of proterties in addition, promptly pattern, spend size, plant height, length of blade and soccer fraud caulome long-pending.Pattern, preferential white, the red and yellow kind selected.Flower diameter is greater than 12 centimetres, and plant height is greater than 1.0 meters.Upright blade or near upright, the soccer fraud belemnoid goes out obviously, and volume is big.
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized
The parent who selects is carried out quadrature, in the hope of obtaining seed.
Quadrature:
Sweet moonlight Cym.Organdy ' Moonlight ' * crescent Cym.Lovely mooncrescent
The flower that selection was bursted forth 12 days is chosen the flower cap with the toothpick of fining away, and pollinium is chosen, and sticks on the muciparous column cap in stamen column top.Hang up label, indicate time and parent's title, put in the greenhouse and cultivate.Control the temp. and humidity environment in greenhouse well, guarantee parent's normal growth.Temperature is 22 ℃, and relative moisture is not less than 20%.
(3), seed asepsis is cultivated
After the artificial supplementary pollination, note observing the ovary situation of change, when capsule presents little Huang, can cut and carry out aseptic seeding.
Alcohol with 70% is cleaned the capsule surface, and (superclean bench) cuts under gnotobasis, takes out seed.With 0.1% mercuric chloride sterilization 23 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate.
Condition of culture: temperature is 24 ℃, and humidity is no more than 70%, intensity of illumination 7000LUX.Medium: MS+6-BA 1.0+IBA 0.1.
(4), filial generation is cultivated
Divide bottle: seed turns green, during the high 2.5cm of bud, promptly carries out the branch bottle on the little protocorm.Every bottle is stayed 6 of plantlets.Constantly divide a bottle subculture with plant strain growth.
Transplant: when height of seedling pushes up bottle cap in the blake bottle (10cm), carry out acclimatization and transplants.Hardening: it is half-open to cultivate bottle cap, puts than (10000LUX) under the intense light irradiation and cultivates 6 days.Throw off bottle cap, hardening is 2 days again, transplants.Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance.Matrix: 800 times of liquid of pine bark+tpn.Put in preceding 15～20 days under the weak light (4000Lux) and cultivate.Increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
(5), according to anti-dried heat-resistant standard screening varieties is carried out in filial generation.
Periodic observation progeny plant growing state, record and evaluation filial generation performance.According to anti-dried heat-resistant standard, emphasis observation plant is selected standard compliant kind at last to the resistance and the adaptability of temperature, humidity.
The experimental data form:
| Relative moisture | Air themperature | Quantity | Growth time | Survival rate |
| 20％ | 35℃～41℃ | 30 | 90 days | 97％ |
| 15％ | 35℃～41℃ | 30 | 45 days | 91％ |
| 5％ | 35℃～41℃ | 30 | 90 days | 43％ |
Embodiment 4:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects
Collect provenance, the provenance of collecting is screened according to anti-dried heat-resistant standard, select the parent.Select the parent to be: sweet moonlight Cym.Organdy ' Moonlight ' and crescent Cym.Lovelymoon crescent
Anti-doing: be meant anti-air drying, promptly relative air humidity is not higher than at 15% o'clock, and the normal growth duration was not less than 45 days.
Heat-resisting: mainly be meant the ability of resisting high temperature summer, promptly when temperature was 35 ℃, the normal growth duration was not less than 45 days (the intermittent processing); When temperature be higher than 35 ℃, when being no more than 41 ℃ (intermittent handle), the normal growth duration was not less than 45 days.
Also to consider 5 pairs of proterties in addition, promptly pattern, spend size, plant height, length of blade and soccer fraud caulome long-pending.Pattern, preferential white, the red and yellow kind selected.Flower diameter is greater than 12 centimetres, and plant height is greater than 1.0 meters.Upright blade or near upright, the soccer fraud belemnoid goes out obviously, and volume is big.
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized
The parent who selects is carried out quadrature, in the hope of obtaining seed.
Reciprocal cross:
Crescent Cym.Lovely moon crescent * sweet moonlight Cym.Organdy ' Moonlight '
The flower that selection was bursted forth 10～15 days is chosen the flower cap with the toothpick of fining away, and pollinium is chosen, and sticks on the muciparous column cap in stamen column top.Hang up label, indicate time and parent's title, put in the greenhouse and cultivate.Control the temp. and humidity environment in greenhouse well, guarantee parent's normal growth.Temperature is 18～27 ℃, and relative moisture is not less than 20%.
(3), seed asepsis is cultivated
After the artificial supplementary pollination, note observing the ovary situation of change, when capsule presents little Huang, can cut and carry out aseptic seeding.
Alcohol with 70% is cleaned the capsule surface, and (superclean bench) cuts under gnotobasis, takes out seed.With 0.1% mercuric chloride sterilization 20～25 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate.
Condition of culture: temperature is 20～26 ℃, and humidity is no more than 70%, intensity of illumination 7000LUX.Medium: MS+6-BA 1.0+IBA 0.1.
(4), filial generation is cultivated
Divide bottle: seed turns green, during the high 2～3cm of bud, promptly carries out the branch bottle on the little protocorm.Every bottle is stayed 5～8 of plantlets.Constantly divide a bottle subculture with plant strain growth.
Transplant: when height of seedling pushes up bottle cap in the blake bottle (8～12cm), carry out acclimatization and transplants.Hardening: it is half-open to cultivate bottle cap, puts than (10000LUX) under the intense light irradiation and cultivates 5～7 days.Throw off bottle cap, hardening is 2～3 days again, transplants.Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance.Matrix: 800 times of liquid of pine bark+tpn.Put in preceding 15～20 days under the weak light (4000Lux) and cultivate.Increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
(5), according to anti-dried heat-resistant standard screening varieties is carried out in filial generation.
Periodic observation progeny plant growing state, record and evaluation filial generation performance.According to anti-dried heat-resistant standard, emphasis observation plant is selected standard compliant kind at last to the resistance and the adaptability of temperature, humidity.
The experimental data form:
| Relative moisture | Air themperature | Quantity | Growth time | Survival rate |
| 20％ | 35℃～41℃ | 30 | 90 days | 97％ |
| 15％ | 35℃～41℃ | 30 | 45 days | 91％ |
| 5％ | 35℃～41℃ | 30 | 90 days | 45％ |
Embodiment 5:
Adopt Baigong master Cym.Enzan Vintage ' Shiranui ' and lucky star Cym.Luckygloria fukunokami quadrature.Other are same as embodiment 1.
Embodiment 6
Adopt Baigong master Cym.Enzan Vintage ' Shiranui ' and lucky star Cym.Luckygloria fukunokami reciprocal cross.Other are same as embodiment 3.
The present invention also can adopt the Da Hua subgenus parent in other the orchid family Cymbidium to hybridize mutually, as: midsummer Cym.Enzan Summer ' Pious ', lucky star Cym.Lucky gloriafukunokami, triumphant girl Cym.Kenny wine color, Baigong master Cym.Enzan Vintage ' Shiranui ' etc.Other parent's selection cross process is identical substantially with said process, no longer repeats at this.
Claims (6)
1, a kind of method for breeding anti-dry heat-resisting Cymbidium hybridum is characterized in that: its selection may further comprise the steps:
(1), according to anti-dried heat-resistant standard provenance is screened and the parent selects;
(2), the anti-parent who does heat-resistant quality that has who step (1) is filtered out is hybridized;
(3), seed asepsis is cultivated;
(4), filial generation is cultivated;
(5), screening varieties is carried out in filial generation, promptly finish seed selection according to anti-dried heat-resistant standard.
2. method for breeding anti-dry heat-resisting Cymbidium hybridum according to claim 1 is characterized in that: the anti-standard of doing in the described step (1) is: relative air humidity is not higher than at 20% o'clock, and the normal growth duration was not less than 45 days; Heat-resistant standard is: when temperature was 35 ℃, the normal growth duration was not less than 45 days; When temperature be higher than 35 ℃, when being no more than 41 ℃, the normal growth duration was not less than 45 days.
3. method for breeding anti-dry heat-resisting Cymbidium hybridum according to claim 1, it is characterized in that: the hybridization that the parent is carried out in the step (2) is quadrature or reciprocal cross, parent after the artificial supplementary pollination soon carries out quadrature or reciprocal cross, and environmental temperature is not less than 18 ℃, and relative moisture is not less than 15%.
4. method for breeding anti-dry heat-resisting Cymbidium hybridum according to claim 3 is characterized in that: after artificial supplementary pollination is finished in the step (2), when capsule presents little Huang, can cut and carry out aseptic seeding.
5. method for breeding anti-dry heat-resisting Cymbidium hybridum according to claim 1 is characterized in that: the concrete steps of described step (3) are: the alcohol of use 70% is cleaned the capsule surface, cuts under gnotobasis, takes out seed; With 0.1% mercuric chloride sterilization 20～25 minutes, aseptic water washing 3 times, seed is seeded in advance on the prepared culture medium, put in the culturing room and cultivate, condition of culture: 20～26 ℃ of temperature, humidity is no more than 70%, intensity of illumination 7000LUX, medium: MS+6-BA 1.0+IBA 0.1.
6. method for breeding anti-dry heat-resisting Cymbidium hybridum according to claim 1 is characterized in that: described step (4) comprising: divide bottle, constantly divide a bottle subculture with plant strain growth; Transplant, when height of seedling pushes up bottle cap and is 8～12cm in the blake bottle, carry out acclimatization and transplants; Hardening, it is half-open to cultivate bottle cap, cultivates under 1000LUX illumination 5～7 days, throws off bottle cap, and hardening is 2～3 days again; Transplant; Clean the medium that adheres on the plantlet, put into the matrix of getting ready in advance; Matrix: 800 times of liquid of pine bark+tpn, under 4000Lux illumination, cultivated in preceding 15～20 days, increase intensity of illumination later on gradually, be advisable to be no more than 20000Lux.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657073A (en) * | 2012-04-20 | 2012-09-12 | 重庆市农业科学院 | Heat-resisting japonica rice breeding method |
| CN102893871A (en) * | 2012-10-24 | 2013-01-30 | 泰安市泰山林业科学研究院 | Method for crossbreeding Cymbidium faberi |
| CN104982314A (en) * | 2015-06-26 | 2015-10-21 | 绵阳市仙龙生物技术有限公司 | Cultivation method for cymbidium |
| CN105580729A (en) * | 2016-01-26 | 2016-05-18 | 南京农业大学 | Culture method of cymbidium hybrid orchids |
| CN113854132A (en) * | 2021-09-07 | 2021-12-31 | 嵩明博源农业科技有限公司 | Cultivation method for promoting cymbidium hybridum seedlings to bloom in advance |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1320853C (en) * | 2005-04-22 | 2007-06-13 | 云南农业大学 | Method for breeding precious cross bred orchid |
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2009
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657073A (en) * | 2012-04-20 | 2012-09-12 | 重庆市农业科学院 | Heat-resisting japonica rice breeding method |
| CN102893871A (en) * | 2012-10-24 | 2013-01-30 | 泰安市泰山林业科学研究院 | Method for crossbreeding Cymbidium faberi |
| CN102893871B (en) * | 2012-10-24 | 2013-11-20 | 泰安市泰山林业科学研究院 | Method for crossbreeding Cymbidium faberi |
| CN104982314A (en) * | 2015-06-26 | 2015-10-21 | 绵阳市仙龙生物技术有限公司 | Cultivation method for cymbidium |
| CN105580729A (en) * | 2016-01-26 | 2016-05-18 | 南京农业大学 | Culture method of cymbidium hybrid orchids |
| CN113854132A (en) * | 2021-09-07 | 2021-12-31 | 嵩明博源农业科技有限公司 | Cultivation method for promoting cymbidium hybridum seedlings to bloom in advance |
| CN113854132B (en) * | 2021-09-07 | 2023-03-14 | 嵩明博源农业科技有限公司 | Cultivation method for promoting early blooming of cymbidium hybridum seedlings |
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