CN107079817A - Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method - Google Patents

Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method Download PDF

Info

Publication number
CN107079817A
CN107079817A CN201710472440.0A CN201710472440A CN107079817A CN 107079817 A CN107079817 A CN 107079817A CN 201710472440 A CN201710472440 A CN 201710472440A CN 107079817 A CN107079817 A CN 107079817A
Authority
CN
China
Prior art keywords
pocket
blue
seedling
lanzhou
tissue culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710472440.0A
Other languages
Chinese (zh)
Inventor
王莲辉
田凡
颜风霞
杨林
姜运力
侯娜
李从瑞
罗在柒
潘得权
廖小锋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUIZHOU PROVINCIAL ACADEMY OF FORESTRY SCIENCES
Original Assignee
GUIZHOU PROVINCIAL ACADEMY OF FORESTRY SCIENCES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GUIZHOU PROVINCIAL ACADEMY OF FORESTRY SCIENCES filed Critical GUIZHOU PROVINCIAL ACADEMY OF FORESTRY SCIENCES
Priority to CN201710472440.0A priority Critical patent/CN107079817A/en
Publication of CN107079817A publication Critical patent/CN107079817A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method is related to the Plant Tissue Breeding mating system of bioengineering, comprises the following steps:A, artificial hybridization pollination;B, aseptic seeding and protocorm sprout the stage;C, Proliferation, Differentiation cultivation stage;D, strengthening seedling and rooting stage;E, hardening and transplanting." excellent pocket blue " combines the merit of parents, compact with plant type, and flower-shape is unique elegant, and pattern is splendid beautiful, it is easy to cultivate, the advantages of average florescence is up to 50 days, it is to avoid spend the long easy lodging of several single and scape and the shortcoming fractureed.Pass through the inventive method; seed germination rate and flowering rate are high; cultivation period is 34 years; it is the high efficiency method for test tube propagation for obtaining a large amount of " excellent pocket is blue " seedlings, high with germination rate, seedling is fast; the advantage such as seedling quality better, growth potential be strong; ordered one test tube seedling can be obtained, and substantial amounts of seedling is provided in a short time, so as to preferably protect wild resource.

Description

Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method
Technical field
The present invention relates to the Plant Tissue Breeding mating system of bioengineering, more particularly to a kind of Lanzhou-Xinjiang kind of pocket is " excellent The tissue culture and rapid propagation method of excellent pocket orchid ".
Background technology
Orchid is one of maximum family of nature, is the hugest and high class of Evolutionary Levels in flowering plant One of group, is only second to composite family among angiosperm and grass family is located at the third-largest section.Global orchid there are about 700 Category, 20000-30000 kinds are distributed widely in the broad area from temperate zone, subtropical zone to the torrid zone.The orchid that China has found and named The category of flower about more than 170 more than 1300 is planted, wherein 2/3 is epiphytic orchid, 1/3 is Terrestrial orchid.At present, whole orchid has been put into 《CITS》Annex I or II.Guizhou is the plateau province of a karst landform extensive development Part, subtropical climate is belonged on geographical environment, with the various and good hydro-thermal of unique complicated topography and geomorphology, vegetation pattern Condition, suitable various plants are multiplied and lived, while also having bred more rich orchid resource.Orchid family plant is distributed with Guizhou Province within the border The category of thing about 76 more than 320 is planted, including substantial amounts of mutation and kind.
Paphiopedilum(Paphiopedilum Pfitz)Plant is one of species of orchid most ornamental value, and pocket is blue Due to unique flower moulding, gorgeous flower color, it is lasting view and admire the florescence and with high ornamental value, the whole world is wide General cultivation.China is one of main place of production of cypripedium, be world's Paphiopedilum important distribution and many novel species mould The formula place of production, there is 18 kinds(Chen, 1999).From in the early 1980s, the cypripedium of China begins to appear in European & American Market; The end of the eighties to the mid-90 is the peak of China's Wild Paphiopedilum platymiscium trade, and pocket orchid market expands rapidly, countless Plant appear in some American-European plantations and flowers market.
Pocket Lanzhou and Xinjiang kind " excellent "( Paphiopedilum GZSLKY Youyou), on 08 17th, 2016 The Royal Horticultural Society(Royal Horticultural Society abbreviations RHS)Log in, and obtain orchid new varieties and step on Remember certificate.The Royal Horticultural Society is that unique, authority orchid new varieties log in mechanism in the world, and the mechanism only logs in head Secondary incubation orchid category between and interspecific hybrid." excellent pocket is blue " is blue with long valve pocket(Paphiopedilum dianthum)For Female parent, leaflet pocket is blue(Paphiopedilum barbigerum)For male parent, the method seed selection of being cross-breeding is formed,
Due to " excellent pocket blue " embryonic development not exclusively, breed difficult in its natural state, it is extremely low to there is seed germination rate, planting percent It is low, the shortcomings of speed of growth is slow;Traditional ramificatiom breeding method, the cycle is long, and breeding coefficient is low, can only breed 1~3 every year Times, Sterile culture difficulty is big.Therefore how to facilitate method quickly to breed " excellent pocket is blue " with plant biological has turned into orchid family plant Thing child care and the emphasis problem of application.
The crossbreeding of Paphiopedilum by Germination Condition due to being limited, and development is very slow, since orchid is sterile After seeding technique application, cypripedium crossbreeding is developed rapidly.But " excellent pocket is blue " belongs to orchid family, Paphiopedilum and planted The new varieties of thing, its tissue culture and rapid propagation method is still in groping, processing stage, and " excellent pocket is blue " seed is extremely small, using other The ripe tissue-culturing rapid propagation method for culturing seedlings of Paphiopedilum, causes abortion rate high, or even the state of stamen abortion occur.And orchid Cultivation period is again long, only hybridization, nursery stage easily will 1-3.
So study a kind of abortion rate it is low, using tissue culture and rapid propagation method, volume facility method for culturing seedlings can be achieved, just turn into " excellent pocket is blue " quickly moves towards market, meets the important prerequisite condition of people's demand.
The content of the invention
To solve " excellent pocket is blue " in the prior art, because embryonic development is incomplete, the technology that difficulty is bred in its natural state is asked A kind of topic, abortion rate of present invention offer is low, can greatly improve the high efficiency method for test tube propagation of breeding coefficient, i.e. pocket Lanzhou-Xinjiang Kind " excellent pocket is blue " tissue culture and rapid propagation method.
Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method, comprises the following steps:
A, artificial hybridization pollination:It is female parent with long valve pocket orchid, leaflet pocket orchid is male parent, the long valve pocket for choosing robust growth is blue female This and male parent carry out artificial supplementary pollination when blooming, 370 to 390 days after pollination, will be developed to and are mature on the whole and uncracked Fruit is used as explant;
B, aseptic seeding and protocorm sprout the stage:From full fruit with alcohol-pickled, it is placed in mercuric chloride solution and sterilizes, Aseptic water washing, cuts fruit with scalpel, brown powder seed is inoculated on culture medium with transfer needle, cultivates 80-100 My god, form protocorm and bud mixture;
C, Proliferation, Differentiation cultivation stage:To obtain more seedlings, bud and protocorm line and staff control are seeded in 1/2MS additions 6- Benzylaminopurine (6-BA), α-naphthylacetic acid(NAA)On culture medium, cultivated through 140-160 days, grow up to grow thickly it is small with short Seedling;
D, strengthening seedling and rooting stage:The clustered plants grown up to are separated, 1/4MS addition α-naphthylacetic acids are inoculated into(NAA)Culture medium On, the intact plant until growing up to tool 2-3 bars root, 3-4 piece leaves.
E, hardening and transplanting:Refined under natural lighting before the bottle outlet when test tube seedling length is to 3-4 centimetres, bottle outlet in greenhouse Seedling, then takes out it from vial, moves into broken bark and mixed-matrix humous;Strengthen temperature, temperature, shelter from heat or light and Ventilating management, " excellent pocket blue " can normal growth, the transplanting survival rate of seedling is up to more than 90%;Periodically pour once it is nitrogenous, The composite fertilizer of phosphorus, potassium, thin fertile duty is applied, and the Lanzhou-Xinjiang kind of pocket " excellent pocket is blue " kind can be set up by strengthening pest and disease damage pipe irrigation project in cultivation The industrialization breeding method system of seedling.
The beneficial effects of the invention are as follows:" excellent pocket is blue " combines the merit of parents, and compact with plant type, flower-shape is only Special elegant, pattern is splendid beautiful, it is easy to cultivate, and the average florescence, what it maintained female parent spent more characteristic and male parent up to 50 days Short scape characteristic, it is to avoid the long easy lodging of several single and scape and the shortcoming fractureed are spent, with high ornamental value.Seed Germination rate and flowering rate are high, and cultivation period is 3-4, are the treasures of great ornamental value in the Lanzhou-Xinjiang product of pocket, and the present invention passes through pocket The tissue culture propagation method research of Lanzhou-Xinjiang kind " excellent pocket is blue ", can improve breeding coefficient, be to obtain a large amount of " excellent pocket is blue " to plant The high efficiency method for test tube propagation of seedling, high with germination rate, seedling is fast, the advantage such as seedling quality better, growth potential are strong, can obtain Ordered one test tube seedling, and provide substantial amounts of seedling in a short time, provides one and has for the seeling industry of " excellent pocket is blue " The approach of effect, so as to preferably protect wild resource.
Brief description of the drawings
The brief description of the drawings of the present invention is as follows:
Fig. 1:Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " picture of blooming;
Fig. 2:Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " cultivation picture.
Embodiment
The present invention is further illustrated below:
Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method, comprises the following steps:
A, artificial hybridization pollination:It is female parent with long valve pocket orchid, leaflet pocket orchid is male parent, the long valve pocket for choosing robust growth is blue female This and male parent carry out artificial supplementary pollination when blooming, 370 to 390 days after pollination, will be developed to and are mature on the whole and uncracked Fruit is used as explant;
B, aseptic seeding and protocorm sprout the stage:From full fruit with alcohol-pickled, disappear in the mercuric chloride solution being placed in Poison, aseptic water washing cuts fruit with scalpel, brown powder seed is inoculated on culture medium with transfer needle, cultivates 80- 100 days, form protocorm and bud mixture;
C, Proliferation, Differentiation cultivation stage:To obtain more seedlings, bud and protocorm line and staff control are seeded in 1/2MS additions 6- Benzylaminopurine (6-BA), α-naphthylacetic acid(NAA)On culture medium, cultivated through 140-160 days, grow up to grow thickly it is small with short Seedling;
D, strengthening seedling and rooting stage:The clustered plants grown up to are separated, 1/4MS addition α-naphthylacetic acids are inoculated into(NAA)Culture medium On, the intact plant until growing up to tool 2-3 bars root, 3-4 piece leaves;
E, hardening and transplanting:Natural lighting lower refining seedling before the bottle outlet when test tube seedling length is to 3-4 centimetres, bottle outlet in greenhouse, so It is taken out from vial afterwards, moved into broken bark and mixed-matrix humous;Strengthen temperature, temperature, shelter from heat or light and divulge information Management, " excellent pocket blue " can normal growth, the transplanting survival rate of seedling is up to more than 90%;Periodically pour once nitrogenous, phosphorus, potassium Composite fertilizer, thin fertile duty applied, and the Lanzhou-Xinjiang kind of pocket " excellent pocket is blue " seedling can be set up by strengthening pest and disease damage pipe irrigation project in cultivation Industrialization breeding method system.
Further, the tissue-cultured seedling of " excellent pocket is blue " is obtained, the crossover process between male parent and female parent is to realize following three The most important step of individual problem:1st, whether the seed obtained is " excellent pocket is blue " new varieties;2nd, the seed that hybridization is obtained is It is no to cultivate " excellent pocket is blue ";Whether " excellent pocket is blue " the 3rd, cultivated reaches quality requirements.Specifically way is:August The first blue season of flowers of pocket, the artificial situation of blooming for controlling to adjust Parent, when maternal long valve pocket orchid bud opens soon, sprinkling is once Concentration promotes long valve pocket orchid to bloom for 1000PPM ethephon (CEPHA),2-(chloroethyl) phosphonic acid, promotes leaflet pocket orchid to bloom with same method again after one week, Bloomed with long valve pocket orchid and carry out artificial supplementary pollination again after starting the clock two weeks, the orchid secretion stigma of this duration valve pocket is largely glued Liquid, leaflet pocket orchid powder is vigourous, and hybridization affinity is strong, best results, completely filled fruit, and survival rate is high after seed tissue culture.Pass through The method, the fruit-setting rate for obtaining " excellent pocket is blue " seed brings up to more than 95%, and maternal, the above-mentioned selection at the florescence of male parent is to carry The deciding factor of high fruit-setting rate.(It is only 20% due to lacking fruit-setting rate under effective insect pollinator, natural conditions), and fruit Full, planting percent is high after seed tissue culture.
Further, described when using alcohol-pickled in step B, from 75% alcohol, soak time is 5min;Rise Sterilization refers to 0.1% mercuric chloride solution in mercury solution, soaks 10 min;Described sterilized water is distilled water or suds.
Further, in step B, described culture medium refers to that 1/2MS adds 6- benzylaminopurines (6-BA) 0.5-1.0 Mg/litre, α-naphthylacetic acid(NAA0.1-0.2 mg/litre culture mediums.Culture medium be tissue-cultured seedling can be formed and grow extremely weigh The factor wanted.And the above-mentioned formula and concentration of 6- benzylaminopurines, α-naphthylacetic acid are even more the pass for determining tissue-cultured seedling success or not Key.
Further, in step B, aseptic seeding and protocorm sprouting stage carry out next step, abortion when cultivating 90 days Rate is minimum.
Further, in step C in culture medium prescription, 6- benzylaminopurines (6-BA) concentration be 2-4 mg/litres, α- Methyl α-naphthyl acetate(NAA)Concentration is 0.1-0.5 mg/litres, and incubation time is to be preferred for 150 days.
Further, in step D, clustered plants are inoculated into 1/4MS addition α-naphthylacetic acids after separating(NAA)0.1-0.5 millis G/l culture medium on, culture grows up to after 55-65 days has 2-3 bars roots, the intact plant of 3-4 piece leaves.
Further, in step E, when test tube seedling length is to 3-4 centimetres, the natural lighting lower refining seedling before bottle outlet in greenhouse 10 days, then it is taken out from vial, clean the culture medium of root, it is 4 to move into broken bark and fertile soil:1 mixed base In matter.Greenhouse keeps sheltering from heat or light and divulging information for 15-28 DEG C of temperature, more than 80% humidity, 70-80%, and " excellent pocket is blue " can be just It is frequently grown, the transplanting survival rate of seedling is up to more than 90%.Pour within usual 15 days or so a nitrogen:Phosphorus:The ratio of potassium is 14:16; 1000 times of 15 composite fertilizer, thin fertile duty is applied, and strengthens pest and disease damage pipe irrigation project in cultivation.
Protection scope of the present invention is not limited only in the technical scheme disclosed in embodiment, all present patent applications respectively The step of technical scheme, the method that tissue-culturing rapid propagation is carried out to " excellent pocket is blue ", and carried out on the basis of above-mentioned technical proposal Improvement, equivalent substitution, each fall within protection scope of the present invention.

Claims (8)

  1. Pocket Lanzhou and Xinjiang 1. kind " excellent pocket is blue " tissue culture and rapid propagation method, comprises the following steps:
    A, artificial hybridization pollination:It is female parent with long valve pocket orchid, leaflet pocket orchid is male parent, the long valve pocket for choosing robust growth is blue female This and male parent carry out artificial supplementary pollination when blooming, 370 to 390 days after pollination, will be developed to and are mature on the whole and uncracked Fruit is used as explant;
    B, aseptic seeding and protocorm sprout the stage:From full fruit with alcohol-pickled, disappear in the mercuric chloride solution being placed in Poison, aseptic water washing cuts fruit with scalpel, brown powder seed is inoculated on culture medium with transfer needle, cultivates 80- 100 days, form protocorm and bud mixture;
    C, Proliferation, Differentiation cultivation stage:To obtain more seedlings, bud and protocorm line and staff control are seeded in 1/2MS additions 6- Benzylaminopurine (6-BA), α-naphthylacetic acid(NAA)On culture medium, cultivated through 140-160 days, grow up to grow thickly it is small with short Seedling;
    D, strengthening seedling and rooting stage:The clustered plants grown up to are separated, 1/4MS addition α-naphthylacetic acids are inoculated into(NAA)Culture medium On, the intact plant until growing up to tool 2-3 bars root, 3-4 piece leaves;
    E, hardening and transplanting:Natural lighting lower refining seedling before the bottle outlet when test tube seedling length is to 3-4 centimetres, bottle outlet in greenhouse, so It is taken out from vial afterwards, moved into broken bark and mixed-matrix humous;Strengthen temperature, temperature, shelter from heat or light and divulge information Management, " excellent pocket blue " can normal growth, the transplanting survival rate of seedling is up to more than 90%;Periodically pour once nitrogenous, phosphorus, potassium Composite fertilizer, thin fertile duty applied, and the Lanzhou-Xinjiang kind of pocket " excellent pocket is blue " seedling can be set up by strengthening pest and disease damage pipe irrigation project in cultivation Industrialization breeding method system.
  2. The Lanzhou-Xinjiang kind of pocket according to claim 1 2. " excellent pocket is blue " tissue culture and rapid propagation method, it is characterised in that:The first pocket of August Blue season of flowers, the artificial situation of blooming for controlling to adjust Parent when maternal long valve pocket orchid bud opens soon, sprays a concentration Promote long valve pocket orchid to bloom for 1000PPM ethephon (CEPHA),2-(chloroethyl) phosphonic acid, promote leaflet pocket orchid to bloom with same method again after one week, with length Valve pocket orchid blooms start the clock two weeks after carry out artificial supplementary pollination again, this duration valve pocket orchid secretion stigma a large amount of mucus, Leaflet pocket orchid powder is vigourous, and hybridization affinity is strong, best results, completely filled fruit, and survival rate is high after seed tissue culture.
  3. The Lanzhou-Xinjiang kind of pocket according to claim 1 3. " excellent pocket is blue " tissue culture and rapid propagation method, it is characterised in that:In step B, Described when using alcohol-pickled, from 75% alcohol, soak time is 5min;Sterilization refers to 0.1% mercuric chloride in mercuric chloride solution Solution, soaks 10 min;Described sterilized water is distilled water or suds.
  4. The Lanzhou-Xinjiang kind of pocket according to claim 1 4. " excellent pocket is blue " tissue culture and rapid propagation method, it is characterised in that:In step B, Described culture medium refers to 1/2MS addition 6- benzylaminopurine (6-BA) 0.5-1.0 mg/litres, α-naphthylacetic acid(NAA0.1- 0.2 mg/litre culture medium.
  5. The Lanzhou-Xinjiang kind of pocket according to claim 1 5. " excellent pocket is blue " tissue culture and rapid propagation method, it is characterised in that:In step B, Aseptic seeding and protocorm sprouting stage carry out next step when cultivating 90 days.
  6. The Lanzhou-Xinjiang kind of pocket according to claim 1 6. " excellent pocket is blue " tissue culture and rapid propagation method, it is characterised in that:In step C In culture medium prescription, 6- benzylaminopurines (6-BA) concentration is 2-4 mg/litres, α-naphthylacetic acid(NAA)Concentration is 0.1-0.5 Mg/litre, incubation time is to be preferred for 150 days.
  7. The Lanzhou-Xinjiang kind of pocket according to claim 1 7. " excellent pocket is blue " tissue culture and rapid propagation method, it is characterised in that:In step D, Clustered plants are inoculated into 1/4MS addition α-naphthylacetic acids after separating(NAA)On the culture medium of 0.1-0.5 mg/litres, 55-65 is cultivated Grow up to tool 2-3 bars root, the intact plant of 3-4 piece leaves after it.
  8. 8. the Lanzhou-Xinjiang kind of pocket " excellent pocket is blue " tissue culture and rapid propagation method according to claim 1-7 any claims, it is special Levy and be:In step E, when test tube seedling length is to 3-4 centimetres, the natural lighting lower refining seedling before bottle outlet in greenhouse 10 days, then It is taken out from vial, the culture medium of root is cleaned, it is 4 to move into broken bark and fertile soil:In 1 mixed-matrix;Greenhouse Keep 15-28 DEG C of temperature, more than 80% humidity, 70-80% to shelter from heat or light and divulge information, " excellent pocket is blue " can normal growth, into The transplanting survival rate of seedling is up to more than 90%;Pour within usual 15 days or so a nitrogen:Phosphorus:The ratio of potassium is 14:16;15 composite fertilizer 1000 times, thin fertile duty is applied, and strengthens pest and disease damage pipe irrigation project in cultivation.
CN201710472440.0A 2017-06-21 2017-06-21 Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method Pending CN107079817A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710472440.0A CN107079817A (en) 2017-06-21 2017-06-21 Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710472440.0A CN107079817A (en) 2017-06-21 2017-06-21 Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method

Publications (1)

Publication Number Publication Date
CN107079817A true CN107079817A (en) 2017-08-22

Family

ID=59605891

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710472440.0A Pending CN107079817A (en) 2017-06-21 2017-06-21 Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method

Country Status (1)

Country Link
CN (1) CN107079817A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108770680A (en) * 2018-05-23 2018-11-09 广东省农业科学院环境园艺研究所 A kind of method of the sapling multiplication and cultivation of red rosy clouds pocket orchid
CN109258463A (en) * 2018-09-18 2019-01-25 广西壮族自治区林业科学研究院 A kind of asexual reproduction method of paphiopedilum armeniacum
CN110089428A (en) * 2019-04-10 2019-08-06 福建省农业科学院作物研究所 A kind of pocket orchid hybrid seed aseptic seeding growing seedlings method
CN110149950A (en) * 2019-06-06 2019-08-23 广西壮族自治区中国科学院广西植物研究所 Improve the method and its test solution of the sub- development quality of white flower pocket orchid species

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102884980A (en) * 2012-09-17 2013-01-23 贵州省林业科学研究院 Quick tissue propagation method of paphiopedilum bellatulum
CN105165622A (en) * 2015-10-12 2015-12-23 临沂大学 Tissue culture rapid propagation method for paph appletonianum

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102884980A (en) * 2012-09-17 2013-01-23 贵州省林业科学研究院 Quick tissue propagation method of paphiopedilum bellatulum
CN105165622A (en) * 2015-10-12 2015-12-23 临沂大学 Tissue culture rapid propagation method for paph appletonianum

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
贵阳晚报: "贵州兜兰 育出新种", 《贵阳晚报》 *
邵莉楣等: "《植物生长调节剂应用手册》", 31 July 2000 *
陈红岩等: "兜兰属植物引种栽培及应用研究", 《中国植物园》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108770680A (en) * 2018-05-23 2018-11-09 广东省农业科学院环境园艺研究所 A kind of method of the sapling multiplication and cultivation of red rosy clouds pocket orchid
CN109258463A (en) * 2018-09-18 2019-01-25 广西壮族自治区林业科学研究院 A kind of asexual reproduction method of paphiopedilum armeniacum
CN109258463B (en) * 2018-09-18 2022-08-02 广西壮族自治区林业科学研究院 Vegetative propagation method of paphiopedilum armeniacum
CN110089428A (en) * 2019-04-10 2019-08-06 福建省农业科学院作物研究所 A kind of pocket orchid hybrid seed aseptic seeding growing seedlings method
CN110149950A (en) * 2019-06-06 2019-08-23 广西壮族自治区中国科学院广西植物研究所 Improve the method and its test solution of the sub- development quality of white flower pocket orchid species
CN110149950B (en) * 2019-06-06 2021-12-03 广西壮族自治区中国科学院广西植物研究所 Method for improving development quality of paphiopedilum albolanum seeds and test solution thereof

Similar Documents

Publication Publication Date Title
CN102187810B (en) Tissue culture propagation method for curcuma soloensis
CN103348920B (en) Rapid propagation method for high quality seedlings of Kyara
CN103461135A (en) Method for propagating hemerocallis hybridus
CN103704130A (en) Chinese orchid and cymbidium hybridum hybrid seedling raising method
CN107079817A (en) Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method
CN102090337A (en) Rapid propagation method of rhododendron latoucheae
CN101180950B (en) Tissue cultivation rapid breeding method of spring dendrobium stem
CN104145814B (en) Method for obtaining regeneration plants by stem tissue culture of cerasus cerasoides (var. cerasoides)
CN106577284B (en) A kind of method of yellow lady's-slipper tissue-culturing rapid propagation and florescence control
CN108401903A (en) A method of improving barley microspores culture callus yield and green seedling
CN101263783B (en) Method for using herbicide barban to fast breed aloe all-male plant strain
CN104221861A (en) Method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue
CN101766123B (en) Method for rapid propagation of zephyr lily
CN105010142A (en) Vietnamese Aquilaria agallocha Roxb tissue culture method
CN102119663A (en) Tissue culture quick propagation technology of clematis mademe Julia correvon
CN102884980B (en) Quick tissue propagation method of paphiopedilum bellatulum
CN106577280A (en) Rapid propagation aseptic seedling by means of tender stem segments and blades of merrillanthus hainanensis
CN100391333C (en) Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum
CN106605596B (en) A method of mass propagation Lycoris aurea is occurred by body embryo
CN105580729A (en) Culture method of cymbidium hybrid orchids
CN102499091B (en) Method for obtaining regeneration plants of petunia hybrida by anther culture
KR101281934B1 (en) Nado Lantern New breed Prince
CN112616675B (en) Tissue culture and rapid propagation method for Zingiber dance
CN108157166A (en) A kind of breed of variety of pocket Lanzhou and Xinjiang and tissue culture and rapid propagation method
CN108112479A (en) A kind of stem section of papaya sprout Bud Differentiation vacantly plants leaf promoting root growth method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170822

RJ01 Rejection of invention patent application after publication