CN109042304A - The selection of blast resisting good quality and high output two-line hybrid rice combination - Google Patents
The selection of blast resisting good quality and high output two-line hybrid rice combination Download PDFInfo
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- CN109042304A CN109042304A CN201811024177.XA CN201811024177A CN109042304A CN 109042304 A CN109042304 A CN 109042304A CN 201811024177 A CN201811024177 A CN 201811024177A CN 109042304 A CN109042304 A CN 109042304A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The present invention relates to a kind of selections of blast resisting good quality and high output two-line hybrid rice combination, belong to Crop Genetic Breeding technical field.This method is related to through Molecular Marker Assisted Selection Technology (MAS), it is selected in conjunction with Other Main Agronomic Characters, wide spectrum, resistance to rice blsat gene Pigm are imported into two-line sterile line, select new blast resisting two-line sterile line, and utilize the blast resisting two-line sterile line and strong advantage, high-quality restorer combo being bred as, the combination of blast resisting good quality and high output two-line hybrid rice is selected, the rice blast resistance of the first generation of hybrid is improved, it is made to meet the needs of current Rice Production.
Description
Technical field
The present invention provides a kind of selection of blast resisting good quality and high output two-line hybrid rice combination, belongs to crop genetic
Breeding technical field.
Background technique
Rice is one of China or even world's staple food crop, high yield, it is high-quality, disease-resistant be the important breeding mesh of rice
Mark.Double-linear hybrid rice is widely used and promotes as a kind of effective way for improving yield and quality of rice, pacifies for grain
Full production makes a great contribution, but limits in production since blast resisting or resistance are not degenerated for commercial variety in the recent period
Using.Therefore, there is an urgent need to improve the rice blast resistance of double-linear hybrid rice, meets the needs of Rice Production.By Pyricularia oryzae
Rice blast caused by (Magnaporthe oryzae) is one of the main fungus disease for threatening the safety in production of world's rice,
The global paddy underproduction as caused by rice blast every year reaches 10%-30%.In the past 40 years, due to the narrow and high cause of parent's hereditary basis
The diversity of characteristic of disease Pyricularia oryzae, rice blast has become hybrid rice and produces the main problem faced, and rice blast exists in recent years
The rice workspaces such as southwest, the middle and lower reach of Yangtze River and northeast continue great outburst, even more bring huge hidden danger to the production of China's rice safety.
It is then the such disease method the most cost-effective of prevention and treatment using resistant gene, cultivation disease-resistant variety.Related rice blast resistance
The correlative studys such as genetic analysis, gene mapping and cloning have more development.From the Yamasaki sixties in last century and
Since Kiyosawa (1966) first reported rice blast resistance gene Pia, Pii and Pik of 3 independent inheritances, up to now,
At least report 88 Major resistance genes in 73 sites, wherein 27 be cloned (China national paddy gene database,
http://www.ricedata.cn/gene；Ma et).Pigm is identified from Chinese resistance to rice blsat kind paddy plum No. 4
A new wide spectrum rice blast resistance gene, the anti-spectrum than generally acknowledged wide spectrum blast resistant gene Pi1, Pi2, Pi3 is wider, right
29 performance highly resistances or immune quilt in the 30 strong pathogenic strain of various regions and nations are located in rice
On No. 6 chromosomes in the section 70kb, positioning and allelism test are chain each other with Pi2, Pi-9, Pi-z, Pi-zt etc. as the result is shown
Or equipotential.In modern breeding, molecular marker assisted selection (MAS) has become a kind of main path for cultivating disease-resistant variety,
In particular with the disease-resistant gene cloned, it can increase the reliability of selection, mitigate breeding work amount and breeding cost.This hair
It is bright to pass through molecular marker assisted selection (MAS) technology, comprehensive agronomy character determination, by wide spectrum, resistance to rice blsat gene Pigm
The grand section 638S of two-line sterile line is imported, new blast resisting two-line sterile line is selected and raises Xian 6S, and then configure blast resisting
Good quality and high output two-line hybrid rice Combination nova has important practical application value to rice blast harm in Rice Production is solved.
Summary of the invention:
The purpose of the present invention is to provide a kind of selections of blast resisting two-line sterile line.
It is a further object of the present invention to provide the selections of blast resisting good quality and high output two-line hybrid rice combination.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of selection of blast resisting two-line sterile line, comprising the following steps:
1) excellent with comprehensive agronomy character, coordinate force is good, but the two-line sterile line parent one of blast resisting resistance does not resist
Rice blast receptor parent, it is miscellaneous with the two blast resisting genetic donor parent of breeding intermediate materials parent of the Pigm containing blast resistant gene
It hands over, plants first generation of hybrid F1, then recurrent parent made with parent one, first backcross generation harvests BC1F1Seed；
2) BC is planted1F1Group by Markers for Detection blast resistant gene Pigm, while investigating Other Main Agronomic Characters,
Select Other Main Agronomic Characters as recurrent parent and the sterile single plant of the Pigm containing blast resistant gene, Ratooning reproduction harvests BC1F2Kind
Son establishes strain；
3) winter plants BC in Hainan1F2, Markers for Detection blast resistant gene Pigm is continued through, spring investigates master
Economical character is wanted, selects Pigm containing blast resistant gene, economical character as the excellent fertile single plant of recurrent parent, harvests BC1F3Kind
Son establishes strain；
4) positive season plants BC in Yangzhou1F3, compared with parent one, comprehensive MAS testing result, Other Main Agronomic Characters, pedigree
Breeding, the sterile single plant of middle choosing, Ratooning reproduction harvest BC1F4Seed continues to establish strain；
5) it proceeds by generations Markers for Detection blast resistant gene Pigm, select Other Main Agronomic Characters as the list of parent one
Strain repeats step 3), 4), until BC1F7, interval sowing is carried out to strain from generation to generation at this, identifies fertility, Other Main Agronomic Characters；Together
Shi Liyong cultivates germ from the rice disease fringe of rice blast resend ward field acquisition natural infection rice blast, selects representative strong
Virulence single-ascospore strain expands and elutes rice blast fungus spore after cultivating, adjusting concentration to 2 × 105 mL-1, used as identification
Inoculum, respectively to stable BC1F7Strain carries out seedling pest, fringe pest blast resistance identification；Comprehensive fertility, Other Main Agronomic Characters,
For blast resistance identification as a result, selection meets the stable strain of breeding objective, Ratooning reproduction is bred as new two system of blast resisting not
It educates and is.
The parent one preferably grand section 639S；The preferably anti-Yang No.6 rice of parent two；The anti-Yang No.6 rice passes through following
Method breeding obtains: making female parent with Yang No.6 rice, makees paternal hybrid with paddy plum No. 4, then make recurrent parent, backcrossing 3 with Yang No.6 rice
Then in generation, was selfed for 4 generations, from BC1F1In generation, starts, and entire cultivating process detects paddy plum No. 4 using molecular labeling InDel 587 and contains
Blast resistant gene Pigm, until BC3F5Generation, selection target rice blast resistance gene homozygosis and other economical characters and yield
Performance and the almost the same strain of Yang No.6 rice obtain the blast resisting height grain weight anti-Yang No.6 rice of two-line hybrid rice restorer；It is anti-
Yang No.6 rice yield level and Yield And Yield Components with to compare Yang No.6 rice suitable, mass of 1000 kernel is stable at 30.5 grams or more；Seedling pest
With the artificial infection idenfication of fringe pest, area is retransmitted throughout the year in rice blast such as Fujian Jianyang, Jinggangshan County, Jiangxi Province, Mt. Huang in Anhui within continuous 3 years
Rice blast identification, Yang No.6 rice show blast resisting, are significantly better than Yang No.6 rice.
The selection further preferably includes step 6): expanding numerous new blast resisting two-line sterile line (BC in Hainan1F8)
Seed, meanwhile, female parent is made with it, makees male parent with different restorers, configures cross combination, identify two system of blast resisting of incubation not
Educate the combo advantage for being.
Preferably with parent one it is maternal in step 1), is that male parent hybridizes with parent two；In step 2), with the work of parent one
Female parent, with F1In generation, makees male parent backcrossing, and only first backcross generation.
Preferably by the rice disease fringe from rice blast resend ward field acquisition natural infection rice blast, culture in step 5)
Germ selects representative strong virus force single-ascospore strain, elutes rice blast fungus spore after expanding culture, adjusts concentration to 2 × 105A mL-1, as inoculum used in identification blast resistance identification.
The two-line sterile line that the blast resisting being bred as according to the method for the invention is bred as preferably raises Xian 6S, feature
Are as follows: indica type temperature sensitive dual purpose genic male sterile line in category；Plant neat and consistent, leaf sheath is colourless, leaf green, and column cap and bran point are colourless；River
Soviet Union sows in spring, and duration from seeding to heading 102 days, full fertility day 138 days, plant height 91cm, leaf number of the main culm 15-16；Tillering ability is stronger, at
Fringe rate is higher, and average the effective fringe 8-9 of single plant, spike length 23cm, average grains per panicle 160, fringe packet neck granule rate 15%, thousand
Weigh 25 grams；Anther slight of stature, pollen abortion thoroughly, based on non-pollen type, sterile degree 100%, sterile plant rate 100%, fertility turn
The origin temp changed is at 23 DEG C hereinafter, sterility is stablized.Total 75% or more the exposing ratio of column cap, wherein bilateral exposing ratio 45% with
On, column cap is big, energetic；Morning spends 85% or more rate, outcrossing seed-setting rate 50%；Resistance: middle bacterial blight-resisting, rice blast are comprehensive
Index 3.2, middle blast resisting；Meter Zhi: brown rice yield 79.0%, head rice rate 65.1%, length-width ratio 3.1, chalky grain rate 21.0%,
Chalkiness degree 2.9%, 2 grades of transparency, 81 millimeters of gel consistence, amylose content 12.9%；Can numerous property it is preferable, the numerous per mu yield 265 in south
Kilogram；Hybrid seed yield is high, raises extensive 1512 production of hybrid seeds with restorer, average yield per mu is at 200 kilograms or more；Combo advantage is not strong, and not
With restorer combo, yield heterosis is obvious and blast resisting.
A kind of selection of blast resisting good quality and high output two-line hybrid rice combination, is included in and is bred as two system of blast resisting
On the basis of sterile line, male parent configuration cross combination is made with strong advantage, high-quality restorer, hybrid generation is guaranteeing yield richer two
Under the premise of excellent No. 4 volume increase, boot stage identifies the neck blast resistance of cross combination, in combination with rice blast sick nursery Resistance Identification knot
Fruit, the rice blast resistance of the cross combination of overall merit configuration；The paddy of all cross combination hybrid generations is harvested, referring to country
" high quality paddy " standard detects the rice quality of all cross combinations, and it is new to obtain blast resisting good quality and high output two-line hybrid rice
Combination.
The blast resisting two-line sterile line is preferably obtained by above-mentioned method breeding；Further preferably raise Xian 6S.
The strong advantage, high-quality restorer preferably raise extensive 1512.
According to selection of the present invention, Xian 6S is raised with blast resisting two-line sterile line raises extensive 1512 with restorer and match
Group is bred as blast resisting good quality and high output two-line hybrid rice Combination nova and raises two excellent 612, and feature is: raising two excellent 612 and belongs to
Indica two line hybrid rice kind；Make a season semilate rice in the middle and lower reach of Yangtze River to plant, the time of infertility 138.0 days, than compareing Feng Liangyou
It is 2.4 days No. four long；Plant height is averaged 129.3cm, spike length 25.7cm, per acre effective fringe 17.1 ten thousand, every total grain panicle number 191.8, knot
Real 82.6% or more rate, 26.0 grams of mass of 1000 kernel；Resistance: rice blast comprehensive resistance index 2 years are respectively 3.1,2.6, panicle blast damage
Mistake rate is 5 grades highest；7 grades of bacterial leaf-blight；9 grades of brown paddy plant hopper；3 grades of heading stage heat resistance；Rice matter main indicator: head rice rate
61.5%, length-width ratio 3.1, chalky grain rate 20%, chalkiness degree 3.1%, gel consistence 66mm, amylose content 19.0%, up to state
3 grades of " high quality paddy " standard high quality of family, the Ministry of Agriculture " edible rice varieties quality " standard high quality are third；Under in participation the Changjiang river in 2016
In trip in ripe late group of green channel regional testing of Xian, 646.35 kilograms of average yield per mu, than compareing rich two excellent No. four volume increase 6.52%；
Continuous examination in 2017, than compareing rich two excellent No. four volume increase 5.09%, reaches extremely significant level by 639.20 kilograms of average yield per mu；The area Liang Nian
Average 642.78 kilograms of output per hectare is tried, than compareing rich two excellent No. four volume increase 5.80%；Pilot production in 2017, average yield per mu
659.04 kilograms, than compareing rich two excellent No. four volume increase 10.11%, reach extremely significant level.
Beneficial effects of the present invention:
The invention patent is a kind of selection of blast resisting good quality and high output two-line hybrid rice combination.This method is related to
To by molecular marker assisted selection (MAS) blast resistant gene Pigm, and the anti-Yang No.6 rice of disease-resistant gene donor is Comprehensive Traits
Excellent breeding intermediate materials avoid bad Linkage drag entrained by original disease-resistant gene donor, shorten breeding time；
In low generation (BC1F1) Other Main Agronomic Characters selection is begun to, and select economical character close to the list of recurrent parent (grand section 638S)
Strain；Advanced lines (BC1F7) fertility, Other Main Agronomic Characters investigation identification are carried out to stable strain, and seedling pest, fringe pest rice are carried out to it
Blast disease resistance identification；Whole process repeats to select sterile single plant Ratooning reproduction in positive season Yangzhou, selects fertile list in spring Hainan
Strain breeding seed, experience Nan Fanbei educate；Finally, comprehensive fertility, Other Main Agronomic Characters, blast resistance identification result are bred as new
Blast resisting two-line sterile line；The sterile line and strong advantage, high-quality restorer combo being bred as using the above method, and to hybridization group
It closes and carries out yield heterosis identification, rice quality detection, fringe pest inoculated identification and rice blast sick nursery Resistance Identification, it is high-quality to be bred as blast resisting
High yield two-line hybrid rice Combination nova.The cross combination has the advantage that blast resistant gene Pigm is passed through molecule mark by (1)
Remember assisted Selection and genetic improvement, expresses that it in the two-line sterile line and Hybrid Rice Combination of incubation, and then blast resisting, have
Benefit protection kind intellectual property.(2) two-line sterile line being bred as, does not contain only blast resistant gene Pigm, and remains receptor parent
This excellent economical character makees cross combination high yield, the stable yields of maternal configuration with it.(3) restorer strong with advantage, high-quality makees father
This, the double-linear hybrid rice Combination nova being bred as with the two-line sterile line combo of Pigm containing blast resistant gene being newly bred as not only resists
Rice blast, yield potentiality are big, and rice quality is excellent, and rice matter is up to " national high quality paddy " standard.
Detailed description of the invention:
Fig. 1: Xian 6S breeding pedigree schematic diagram is raised for blast resisting two-line sterile line of the invention
Fig. 2: two excellent 612 breeding process schematic diagrames are raised for blast resisting good quality and high output two-line hybrid rice kind of the invention
Specific embodiment:
A kind of selection for blast resisting good quality and high output two-line hybrid rice combination that the following is further explained with reference to the attached drawings.
Embodiment 1
1, breeding material:
1) made with grand section 638S maternal.Grand section 638S is the two-line sterile line that Hunan Ya Huazhong industry research institute cultivates.
The kind applies and authorizes to Plant new variety protection office of the Ministry of Agriculture, and kind power number: CNA20090950.7 can be purchased from
Hunan Ya Huazhong industry research institute.The kind is the two-line sterile line that China promotes the use of a large area in recent years, prepared miscellaneous
Rice is handed over to have many advantages, such as that yield is high, quality is excellent, wide adaptability.
2) anti-Yang No.6 rice (Yang No.6 rice × Gu Mei 4, BC3F5) make male parent.Wherein, Yang No.6 rice is Lixiahe region in Jiangsu
What Institute of agricultural sciences was cultivated.The kind applies and authorizes to Plant new variety protection office of the Ministry of Agriculture, kind power number:
CAN20000091.8 can be purchased from Jiangsu gold soil Zhong Ye Co., Ltd.The kind is that the backbone of China's two-line hybrid rice restores
System, prepared hybrid paddy rice have many advantages, such as that yield is high, quality is excellent, wide adaptability.Paddy plum No. 4 are China place rice varieties,
Rice blast resistance gene Pigm is carried, is the elite germplasm of China's performance wide spectrum, lasting rice blast resistance.It is detailed in: Deng Y,
Zhai K,Xie Z,Yang D,Zhu X,Liu J,Wang X,Qin P,Yang Y,Zhang G,Li Q,Zhang J,Wu
S,Milazzo J,Mao B,Wang E,Xie H,Tharreau D,He Z.Epigenetic regulation of
antagonistic receptors confers rice blast resistance with yield
balance.Science,2017,355:1-4.Anti- Yang No.6 rice is Inst. of Agricultural Science, Lixiahe Prefecture, Jiangsu Prov. with Yang No.6 rice
Make female parent, makees paternal hybrid with paddy plum No. 4, then recurrent parent is made with Yang No.6 rice, be returned for 3 generations, be then selfed for 4 generations, from BC1F1Generation
Starting, entire cultivating process detects the paddy plum No. 4 blast resistant gene Pigm contained using molecular labeling InDel 587, until
BC3F5In generation, selection target rice blast resistance gene is homozygous and other economical characters and yield show and Yang No.6 rice is almost the same
Strain obtains the blast resisting height grain weight anti-Yang No.6 rice of two-line hybrid rice restorer.Anti- Yang No.6 rice yield level and yield structure
At the factor with to compare Yang No.6 rice suitable, mass of 1000 kernel is stable at 30.5 grams or more；The artificial infection idenfication of seedling pest and fringe pest, even
The identification of area's rice blast, Yang No.6 rice performance are retransmitted throughout the year in rice blast such as Fujian Jianyang, Jinggangshan County, Jiangxi Province, Mt. Huang in Anhui within continuous 3 years
Blast resisting is significantly better than control Yang No.6 rice.
2, the breeding of blast resisting two-line sterile line, such as attached drawing 1:
1) excellent with comprehensive agronomy character, coordinate force is good, but the grand section 638S of two-line sterile line of not blast resisting resistance makees
Female parent, with the anti-Yang No.6 rice of breeding intermediate materials of the Pigm containing blast resistant gene (Yang No.6 rice × Gu Mei 4, BC3F5) make male parent
First generation of hybrid F is planted in hybridization1, then recurrent parent made with grand section 638S, first backcross generation harvests BC1F1Seed；
2) BC is planted1F140 plants of group, by Markers for Detection blast resistant gene Pigm, detection is labeled as InDel
587 (primer sequences are as follows: 5'-ACTTGCTGGGAGAAGGATT-3'(SEQ ID NO.1) and 5'-
AGTTCGTACTTTTCAGGCT-3'(SEQ ID NO.2)), while Other Main Agronomic Characters are investigated, select Other Main Agronomic Characters picture
Sterile single plant 18 of recurrent parent and the Pigm containing blast resistant gene, Ratooning reproduction harvest BC1F2Seed establishes strain；
3) winter plants BC in Hainan1F2, Markers for Detection blast resistant gene Pigm is continued through, spring investigates master
Economical character is wanted, excellent 12 plants of the fertile single plant of Pigm containing blast resistant gene, economical character as grand section 638S, harvest are selected
BC1F3Seed establishes strain；
4) positive season sows BC in Yangzhou1F3, compared with grand section 638S, comprehensive MAS testing result investigates Main Agronomic
Shape, pedigree breeding is middle to select 9 sterile single plants, and Ratooning reproduction harvests BC1F4Seed continues to establish strain；
5) it proceeds by generations Markers for Detection blast resistant gene Pigm, select Other Main Agronomic Characters as the list of grand section 638S
Strain repeats step 3), 4), until BC1F7, interval sowing is carried out to strain from generation to generation at this, identifies fertility, Other Main Agronomic Characters；Together
Rice of the Shi Liyong from the rice blast resend ward field such as Guangdong, Hubei, Hainan, Zhejiang and Jiangsu acquisition natural infection rice blast
Sick fringe, cultivate germ, select 5 representativeness strong virus force single-ascospore strain GD18-3 (B1, pathogenicity rate 65.6%), ES9-5-1 (B15,
Pathogenicity rate 75.0%), hnn1-1 (C1, pathogenicity rate 59.4%), (G1 is caused by ZJ3-4 (A15, pathogenicity rate 63.3%) and XK8-4-4
Sick rate 37.5%), expand and elutes rice blast fungus spore after cultivating, adjusting concentration to 2 × 105 mL-1, used as identification
Inoculum, respectively to stable BC1F7Strain carries out seedling pest, fringe pest blast resistance identification；Comprehensive fertility, Other Main Agronomic Characters,
Blast resistance identification meets breeding objective, can be used as new two system of blast resisting not as a result, the whole characters of strain S points 6 are excellent
It educates and is.
6) expand numerous strain S points of 6 (BC in Hainan1F8), and named as to raise Xian 6S, meanwhile, made with it is maternal, with not
Make male parent with restorer, configures cross combination, identify that the blast resisting two-line sterile line of incubation raises the combo advantage of Xian 6S.
7) Xian 6S is raised according to the blast resisting two-line sterile line that above method is bred as, it is special with following sterility and feature
Property:
Sterility: raising Xian 6S and identify under the natural conditions of Yangzhou, and sterile stationary phase, anther was thin 20 days-August 30th July
It is small, pollen abortion thoroughly, based on non-pollen type, sterile degree 100%, sterile plant rate 100%, bagging self-fruitful rate be 0, belong to
In the lower dual purpose genic male sterile line of origin temp.The identification of 2017 China Paddy Rice Inst's phjytotrons: raising Xian 6S is temperature sensitive two
With line with genic sterile, the origin temp of Fertility al-teration is at 23 DEG C hereinafter, meeting ministry standard.
Economical character: raising Xian 6S plant neat and consistent, and leaf sheath is colourless, leaf green, and column cap and bran point are colourless.Yangzhou May
It sows within 10th, duration from seeding to heading 102 days or so, full fertility day 138 days or so, plant height 91cm or so, leaf number of the main culm 15-16
?.Tillering ability is stronger, and the percentage of earbearing tiller is higher, and average the effective fringe 8-9 of single plant, spike length 23cm or so, average grains per panicle 160
Left and right, fringe packet neck granule rate 15% or so, 25 grams of mass of 1000 kernel or so.
Flowering habit: it raises Xian 6S mono- the Honoka phase 7-8 days or so, the single plant florescence 10 days or so.The 3-6 days after heading, account for total
80% or so of the amount of blooming, the spreading angle of grain husk flower are 30 ° or so, single grain husk the flowers are in blossom to number luffing when being closed be 80-120 points
Clock.Under the conditions of fair weather, begin when the morning 8:30 flower, is peak of blooming day when 9:30-12:00, morning 85% or more rate of flower,
Column cap total exposed 75% or more, wherein 45% or more double exposing ratios.In the case where bagging artificial pollination, outcrossing seed-setting rate can
Up to 70% or so；In seed farm, outcrossing seed-setting rate is up to 50% or so.To " 920 " sensitivity.
Rice quality: according to detection: it is white to raise Xian 6S brown rice yield 79.0%, head rice rate 65.1%, chalky grain rate 21.0%, chalk
Spend 2.9%, length-width ratio 3.1, amylose content 12.9%, gel consistence 8mm, 2 grades of transparency.
Resistance: inoculated identification raises bacterial blight-resisting in Xian 6S；2011-2013 is in enshi, Jiangxi Suichuan, Anhui
The rice blast such as Jinzhai County, Shanghang, Fujian retransmit the identification of area's spontaneous induction throughout the year, and composite index is below 5；Continuous 2 years with Guangdong,
Hubei, Hainan, 5 planted in different ecological areas in Zhejiang and Jiangsu rice blast strong pathological form representative strain mixing after artificial infection idenfication,
Seedling pest blast resisting, resists in performance by 3 grades of fringe pest or so.
Crossbreeding characteristic: can numerous property it is preferable, the spring in 2013 is bred in Hainan, 263.5 kilograms of per mu yield；Hybrid seed yield
Height, continuous 2 years of 2013-2014 raise Xian 6S/ in Yangzhou and raise the extensive 1512 equal combination production of hybrid seeds, average yield per mu 200 kilograms with
On.
Combo advantage is strong: with Xian 6S and different restorer combos is raised, yield heterosis is obvious, and all combinations all relatively compare rich two
Excellent No. four volume increase, and blast resisting.
3, blast resisting good quality and high output two-line hybrid rice Combination nova raises two excellent 612 breeding, such as attached drawing 2:
1) make female parent to raise Xian 6S, make male parent to raise extensive 1512, combo is bred as blast resisting, good quality and high output double-line hybrid water
Rice Combination nova raises two excellent 612.Positive season in 2013 carries out advantage identification in Yangzhou, has outstanding performance, and boot stage is inoculated with Pyricularia oryzae, fringe
Pest 3.0 or so, middle blast resisting；2014-2015 carry out variety comparative test, and enshi, Jiangxi Suichuan, Anhui Jinzhai County,
The rice blast such as Shanghang, Fujian retransmit the identification of area's spontaneous induction throughout the year, rice blast composite index 3.0 or so, show it is excellent, rich,
Anti-, wide adaptability.2016-2017 participates in ripe late group of green channel regional testing of Xian in the middle and lower reach of Yangtze River of national South Rice Region of China,
Ripe late group of green channel pilot production of Xian in the middle and lower reach of Yangtze River of the country South Rice Region of China of participation in 2017, while large-scale demonstration kind
It plants.Pass through national crop varietal approval committee within 2018.
2) two excellent 612 feature is raised
Stable high yield, yield potentiality are big:
2016-2017 is participated in the middle and lower reach of Yangtze River of national South Rice Region of China in ripe late group of green channel regional testing of Xian,
2016 646.35 kilograms of annual per mu yields reach extremely significant level than compareing rich two excellent No. four volume increase 6.52%；2017 annual mus
639.20 kilograms are produced, than compareing rich two excellent No. four volume increase 5.09%, reaches extremely significant level；Try average output per hectare in the area Liang Nian
642.78 kilograms, than compareing rich two excellent No. four volume increase 5.80%；Pilot production in 2017, compares by 659.04 kilograms of average yield per mu
According to rich two excellent No. four volume increase 10.11%, extremely significant level is reached.
Best in quality, commodity is good:
Rice matter main indicator: head rice rate 61.5%, length-width ratio 3.1, chalky grain rate 20%, chalkiness degree 3.1%, gel consistence
66mm, amylose content 19.0%, up to national 3 grades of " high quality paddy " standard high quality, the Ministry of Agriculture " edible rice varieties quality " mark
It is quasi- high-quality third.
Blast resisting:
Rice blast comprehensive resistance index 2 years are respectively 3.1,2.6, and panicle blast loss late is 5 grades highest.
Economical character is excellent, and applicability is wide:
Seedling is short strong, and the short and thick green of leaf sheath, blade is straight and upright, and leaf color is dark green, and stooling is stronger；Strain plant type is compacter, strain
The average 129.3cm of height, top three open that functional leaf is straight and upright, and light posture is good, lift on sword-like leave when mature, bar blueness seed yellow maturity lover；
Stooling is strong, fringe type is big, spike length 25.7cm, per acre effective fringe 17.1 ten thousand, every total grain panicle number 191.8, setting percentage 82.6% with
On, 26.0 grams of mass of 1000 kernel；It is the time of infertility 138.0 days, excellent 2.4 days No. four longer than compareing rich two, belong to middle Xian ripe double-line hybrid late
Rice type.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (10)
1. a kind of selection of blast resisting two-line sterile line characterized by comprising
1) excellent with comprehensive agronomy character, coordinate force is good, but the two-line sterile line parent one of blast resisting resistance does not make anti-rice blast
Ospc gene receptor parent makees blast resistant gene donor parents with the breeding intermediate materials parent two of the Pigm containing blast resistant gene
First generation of hybrid F is planted in hybridization1, then recurrent parent made with parent one, first backcross generation harvests BC1F1Seed；
2) BC is planted1F1Group by Markers for Detection blast resistant gene Pigm, while investigating Other Main Agronomic Characters, selection
Other Main Agronomic Characters harvest BC as recurrent parent and the sterile single plant of the Pigm containing blast resistant gene, Ratooning reproduction1F2Seed,
Establish strain；
3) winter plants BC in Hainan1F2, Markers for Detection blast resistant gene Pigm is continued through, spring investigates main agriculture
Skill character selects Pigm containing blast resistant gene, economical character as the excellent fertile single plant of parent one, harvests BC1F3Seed is built
Vertical strain；
4) positive season plants BC in Yangzhou1F3, compared with parent one, comprehensive MAS testing result investigates Other Main Agronomic Characters, pedigree
Breeding, the sterile single plant of middle choosing, Ratooning reproduction harvest BC1F4Seed continues to establish strain；
5) single plant of Markers for Detection blast resistant gene Pigm, selection Other Main Agronomic Characters as parent one, weight are proceeded by generations
Multiple step 3), 4), until BC1F7, interval sowing is carried out to strain from generation to generation at this, identifies fertility, Other Main Agronomic Characters；The same time-division
It is other to stable BC1F7Strain carries out seedling pest, fringe pest blast resistance identification；Comprehensive fertility, Other Main Agronomic Characters, rice blast are anti-
Property qualification result, selection meets the stable strain of breeding objective, and Ratooning reproduction is bred as new blast resisting two-line sterile line.
2. selection according to claim 1, it is characterised in that the parent one is grand section 639S；Parent two is anti-
Yang No.6 rice；Breeding obtains the anti-Yang No.6 rice by the following method: making female parent with Yang No.6 rice, it is miscellaneous to make male parent with paddy plum No. 4
It hands over, then recurrent parent is made with Yang No.6 rice, be returned for 3 generations, be then selfed for 4 generations, from BC1F1In generation, starts, and entire cultivating process, which utilizes, to be divided
Son label InDel 587 detects the paddy plum No. 4 blast resistant gene Pigm contained, until BC3F5Generation, selection target rice blast are anti-
Property gene pure and other economical characters and yield performance with the almost the same strain of Yang No.6 rice, obtain blast resisting height grain weight
The anti-Yang No.6 rice of two-line hybrid rice restorer；Anti- Yang No.6 rice yield level and Yield And Yield Components with to compare Yang No.6 rice suitable,
Mass of 1000 kernel is stable at 30.5 grams or more；The artificial infection idenfication of seedling pest and fringe pest, continuous 3 years in Fujian Jianyang, Jiangxi Jing Gang
The rice blast such as mountain, Mt. Huang in Anhui retransmit the identification of area's rice blast throughout the year, and Yang No.6 rice shows blast resisting, is significantly better than Yang No.6 rice.
3. selection according to claim 1 or 2, it is characterised in that the selection further includes step 6):
Numerous new blast resisting two-line sterile line (BC is expanded in Hainan1F8) seed, meanwhile, female parent is made with it, makees male parent with different restorers,
Cross combination is configured, identifies the combo advantage of the blast resisting two-line sterile line of incubation.
4. selection according to claim 1 or 2, it is characterised in that be female parent, with parent with parent one in step 1)
Two are hybridized for male parent；In step 2), female parent is made with parent one, with F1In generation, makees male parent backcrossing, and only first backcross generation.
5. selection according to claim 1 or 2, it is characterised in that: in step 5), using from rice blast resend ward
Field acquires the rice disease fringe of natural infection rice blast, cultivates germ, representative strong virus force single-ascospore strain is selected, after expanding culture
Rice blast fungus spore is eluted, adjusts concentration to 2 × 105A mL-1, as inoculum used in identification blast resistance identification.
6. selection according to claim 1 or 2, which is characterized in that method according to claim 1 or claim 2 is bred as
Blast resisting be bred as two-line sterile line be raise Xian 6S, feature are as follows: indica type temperature sensitive dual purpose genic male sterile line in category；Plant
Neat and consistent, leaf sheath is colourless, leaf green, and column cap and bran point are colourless；Jiangsu is sowed in spring, and duration from seeding to heading 102 days, full fertility day
138 days, plant height 91cm, leaf number of the main culm 15-16；Tillering ability is stronger, and the percentage of earbearing tiller is higher, and average the effective fringe 8-9 of single plant, fringe
Long 23cm, average grains per panicle 160, fringe packet neck granule rate 15%, 25 grams of mass of 1000 kernel；Anther slight of stature, pollen abortion thoroughly, with
Based on non-pollen type, sterile degree 100%, sterile plant rate 100%, the origin temp of Fertility al-teration is at 23 DEG C hereinafter, sterility is steady
It is fixed.Total 75% or more the exposing ratio of column cap, wherein 45% or more bilateral exposing ratio, column cap is big, energetic；Morning flower rate 85% with
On, outcrossing seed-setting rate 50%；Resistance: middle bacterial blight-resisting, rice blast composite index 3.2, middle blast resisting；Meter Zhi: brown rice yield
79.0%, head rice rate 65.1%, length-width ratio 3.1, chalky grain rate 21.0%, chalkiness degree 2.9%, 2 grades of transparency, gel consistence 81
Millimeter, amylose content 12.9%；Can numerous property it is preferable, south it is numerous 265 kilograms of per mu yield；Hybrid seed yield is high, raises with restorer extensive
1512 production of hybrid seeds, average yield per mu is at 200 kilograms or more；Combo advantage is strong, and different restorer combos, and yield heterosis is obvious and anti-rice
Seasonal febrile diseases.
7. a kind of selection of blast resisting good quality and high output two-line hybrid rice combination, it is characterised in that be included in and be bred as anti-rice
On the basis of seasonal febrile diseases two-line sterile line, male parent configuration cross combination is made with strong advantage, high-quality restorer, hybrid generation is guaranteeing to produce
Under the premise of measuring richer two excellent No. 4 volume increase, boot stage identifies the neck blast resistance of cross combination, in combination with rice blast sick nursery resistance
Qualification result, the rice blast resistance of the cross combination of overall merit configuration；The paddy of all cross combination hybrid generations is harvested, is joined
According to national " high quality paddy " standard, the rice quality of all cross combinations is detected, obtains blast resisting good quality and high output double-line hybrid
Long-grained nonglutinous rice Combination nova.
8. selection according to claim 7, it is characterised in that the blast resisting two-line sterile line passes through right
It is required that method breeding described in 1 or 2 obtains；It is preferred that raising Xian 6S described in claim 6.
9. selection according to claim 8, it is characterised in that the strong advantage, high-quality restorer be raise it is extensive
1512。
10. the selection according to any one of claim 7~9, it is characterised in that according to the selection, with
Blast resisting two-line sterile line raises Xian 6S and restorer raises extensive 1512 combo, is bred as blast resisting good quality and high output two-line hybrid rice
Combination nova raises two excellent 612, and feature is: raising two excellent 612 and belongs to Indica two line hybrid rice kind；Make in the middle and lower reach of Yangtze River
The plantation of one season semilate rice, it is the time of infertility 138.0 days, excellent 2.4 days No. four longer than compareing rich two；Plant height is averaged 129.3cm, spike length
25.7cm, effective fringe 17.1 ten thousand per acre, every total grain panicle number 191.8,82.6% or more setting percentage, 26.0 grams of mass of 1000 kernel；Resistance:
Rice blast comprehensive resistance index 2 years are respectively 3.1,2.6, and panicle blast loss late is 5 grades highest；7 grades of bacterial leaf-blight；Brown paddy plant hopper 9
Grade；3 grades of heading stage heat resistance；Rice matter main indicator: head rice rate 61.5%, length-width ratio 3.1, chalky grain rate 20%, chalkiness degree
3.1%, gel consistence 66mm, amylose content 19.0%, up to national 3 grades of " high quality paddy " standard high quality, the Ministry of Agriculture " edible rice
Variety and quality " standard high quality is third；In the participation middle and lower reach of Yangtze River in 2016 in ripe late group of green channel regional testing of Xian, average mu
646.35 kilograms are produced, than compareing rich two excellent No. four volume increase 6.52%；Continuous examination in 2017,639.20 kilograms of average yield per mu, than control
Rich two excellent No. four volume increase 5.09%, reach extremely significant level；The area Liang Nian tries average 642.78 kilograms of output per hectare, richer by two than compareing
Excellent No. four volume increase 5.80%；Pilot production in 2017,659.04 kilograms of average yield per mu, than compareing rich two excellent No. four volume increase
10.11%, reach extremely significant level.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618916A (en) * | 2019-02-02 | 2019-04-16 | 福建农林大学 | A kind of selection of long grain odor type High quality glutinous rice conventional variety |
| CN112586340A (en) * | 2020-12-04 | 2021-04-02 | 国家粳稻工程技术研究中心 | New combined breeding method for high-quality eurytopic hybrid rice |
| CN113179884A (en) * | 2021-04-29 | 2021-07-30 | 山东省农业科学院作物研究所 | Method for cultivating high-yield wheat with gibberellic disease resistance in Huanghuai wheat area based on polygene polymerization |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004066719A1 (en) * | 2003-01-31 | 2004-08-12 | Plant Genome Center Co., Ltd. | Method of quickly breeding improved plant variety |
| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN103250635A (en) * | 2013-05-31 | 2013-08-21 | 江苏沿海地区农业科学研究所 | Breeding method and seed production method of high-grain-weight and high-quality two-line hybrid rice |
| CN103497996A (en) * | 2013-09-22 | 2014-01-08 | 江苏里下河地区农业科学研究所 | Molecular marker InDe1587 for detecting rice blast resistant gene Pigm(t) of rice #4 |
| CN103598085A (en) * | 2013-10-28 | 2014-02-26 | 江苏沿海地区农业科学研究所 | Breeding method of photo-thermo-sensitive genic male sterile line of quality rice |
| CN103975846A (en) * | 2014-05-15 | 2014-08-13 | 江西省农业科学院水稻研究所 | Novel method for creating dual-purpose rice genic male sterile line |
| CN104969855A (en) * | 2015-07-30 | 2015-10-14 | 江苏里下河地区农业科学研究所 | Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance |
| WO2016101859A1 (en) * | 2014-12-22 | 2016-06-30 | 广东省农业科学院植物保护研究所 | Rice blast resistant gene pi50, preparation methods therefor, and applications thereof |
| CN106636354A (en) * | 2016-11-17 | 2017-05-10 | 福建农林大学 | Method for increasing disease-resistant breeding efficiency of hybrid rice |
| CN107306786A (en) * | 2017-04-25 | 2017-11-03 | 江苏里下河地区农业科学研究所 | A kind of breeding method of the anti-fall double-linear hybrid rice kind of high-grain weight excellent-quality blast resisting |
| US20180206426A1 (en) * | 2016-09-29 | 2018-07-26 | Wuhan Polyploid Biotechnology Co. Ltd | Polyploid two-line hybrid rice and breeding method thereof |
-
2018
- 2018-09-04 CN CN201811024177.XA patent/CN109042304B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004066719A1 (en) * | 2003-01-31 | 2004-08-12 | Plant Genome Center Co., Ltd. | Method of quickly breeding improved plant variety |
| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN103250635A (en) * | 2013-05-31 | 2013-08-21 | 江苏沿海地区农业科学研究所 | Breeding method and seed production method of high-grain-weight and high-quality two-line hybrid rice |
| CN103497996A (en) * | 2013-09-22 | 2014-01-08 | 江苏里下河地区农业科学研究所 | Molecular marker InDe1587 for detecting rice blast resistant gene Pigm(t) of rice #4 |
| CN103598085A (en) * | 2013-10-28 | 2014-02-26 | 江苏沿海地区农业科学研究所 | Breeding method of photo-thermo-sensitive genic male sterile line of quality rice |
| CN103975846A (en) * | 2014-05-15 | 2014-08-13 | 江西省农业科学院水稻研究所 | Novel method for creating dual-purpose rice genic male sterile line |
| WO2016101859A1 (en) * | 2014-12-22 | 2016-06-30 | 广东省农业科学院植物保护研究所 | Rice blast resistant gene pi50, preparation methods therefor, and applications thereof |
| CN104969855A (en) * | 2015-07-30 | 2015-10-14 | 江苏里下河地区农业科学研究所 | Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance |
| US20180206426A1 (en) * | 2016-09-29 | 2018-07-26 | Wuhan Polyploid Biotechnology Co. Ltd | Polyploid two-line hybrid rice and breeding method thereof |
| CN106636354A (en) * | 2016-11-17 | 2017-05-10 | 福建农林大学 | Method for increasing disease-resistant breeding efficiency of hybrid rice |
| CN107306786A (en) * | 2017-04-25 | 2017-11-03 | 江苏里下河地区农业科学研究所 | A kind of breeding method of the anti-fall double-linear hybrid rice kind of high-grain weight excellent-quality blast resisting |
Non-Patent Citations (5)
| Title |
|---|
| 何奎等: "两系杂交水稻新组合隆两优281", 《杂交水稻》 * |
| 农业农村部: "扬两优612", 《中华人民共和国农业农村部公告第65号》 * |
| 向聪等: "分子标记辅助选择改良C815S的稻瘟病抗性", 《湖南农业大学学报(自然科学版)》 * |
| 李育红等: "杂交籼稻稻瘟病抗性分子标记辅助育种的探索与实践", 《扬州大学学报（农业与生命科学版）》 * |
| 殷得所等: "抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用", 《中国水稻科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618916A (en) * | 2019-02-02 | 2019-04-16 | 福建农林大学 | A kind of selection of long grain odor type High quality glutinous rice conventional variety |
| CN112586340A (en) * | 2020-12-04 | 2021-04-02 | 国家粳稻工程技术研究中心 | New combined breeding method for high-quality eurytopic hybrid rice |
| CN113179884A (en) * | 2021-04-29 | 2021-07-30 | 山东省农业科学院作物研究所 | Method for cultivating high-yield wheat with gibberellic disease resistance in Huanghuai wheat area based on polygene polymerization |
| CN113179884B (en) * | 2021-04-29 | 2022-05-10 | 山东省农业科学院作物研究所 | Method for cultivating high-yield wheat with gibberellic disease resistance in Huanghuai wheat area based on polygene polymerization |
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