CN109042304B - Breeding method of rice blast resistant high-quality high-yield two-line hybrid indica rice combination - Google Patents
Breeding method of rice blast resistant high-quality high-yield two-line hybrid indica rice combination Download PDFInfo
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Abstract
The invention relates to a breeding method of a rice blast resistant high-quality high-yield two-line hybrid indica rice combination, belonging to the technical field of crop genetic breeding. The method relates to a method for breeding a new rice blast resistant two-line sterile line by introducing broad-spectrum and lasting rice blast resistant gene Pigm into the two-line sterile line through a molecular marker assisted selection technology (MAS) and combining with main agronomic character selection, and breeding a rice blast resistant high-quality and high-yield two-line hybrid indica rice combination by matching the bred rice blast resistant two-line sterile line with a strong-superiority and high-quality restorer line, so that the rice blast resistance of a first hybrid generation is improved, and the current rice production requirements are met.
Description
Technical Field
The invention provides a breeding method of a rice blast resistant high-quality high-yield two-line hybrid indica rice combination, belonging to the technical field of crop genetic breeding.
Background
Rice is one of the main grain crops in China and even the world, and high yield, high quality and disease resistance are important breeding targets of the rice. The two-line hybrid rice is widely applied and popularized as an effective way for improving the yield and the quality of the rice, makes great contribution to the safe production of grains, but limits the application in the production due to the fact that the popularized variety is not resistant to rice blast or resistance degeneration in the recent time. Therefore, there is an urgent need to improve the blast resistance of two-line hybrid rice to meet the demand for rice production. The rice blast caused by the Magnaporthe oryzae is one of the most main fungal diseases threatening the safe production of rice in the world, and the yield reduction of rice caused by the rice blast reaches 10 to 30 percent every year in the world. In recent 40 years, rice blast has become a main problem in hybrid rice production due to narrow genetic basis of parents and diversity of highly pathogenic rice blast germs, and in recent years, rice blast continuously and greatly outbreaks occur in rice growing areas such as southwest, middle and downstream of Yangtze river, northeast and the like, and further great hidden danger is brought to the safe production of rice in China. The most economic and effective method for preventing and treating the diseases is to use the resistance genes and cultivate disease-resistant varieties. Related researches on genetic analysis, gene localization, cloning and the like of rice blast resistance have been carried out more. Since 3 independently inherited rice blast resistance genes Pia, Pii and Pik were first reported by Yamasaki and Kiyosawa (1966) in the last 60's century, at least 88 major resistance genes of 73 sites were reported by now, 27 of which were cloned (Chinese national Rice Gene database, http:// www.ricedata.cn/gene; Ma et). Pigm is a new broad-spectrum rice blast resistance gene identified from a Chinese durable rice blast resistant variety, namely, flos Pruni mume No. 4, has a wider resistance spectrum than the accepted broad-spectrum rice blast resistant genes Pi1, Pi2 and Pi3, shows high resistance or immune quilt for 29 of 30 strong pathogenic strains collected from different regions and countries, is positioned in a 70kb interval on a 6 th chromosome of rice, and the positioning and allelism test result shows that the Pigm and Pi2, Pi-9, Pi-z, Pi-zt and the like are mutually linked or allelism. In modern breeding, molecular Marker Assisted Selection (MAS) has become a main approach for breeding disease-resistant varieties, and particularly, cloned disease-resistant genes are utilized to increase the reliability of selection and reduce the breeding workload and the breeding cost. The invention synthesizes agronomic character selection by a molecular Marker Assisted Selection (MAS) technology, introduces broad-spectrum and durable rice blast resistance gene Pigm into two-line sterile line clone 638S, breeds a new rice blast resistance two-line sterile line Yangxian 6S, and further configures a new rice blast resistance high-quality high-yield two-line hybrid indica rice combination, thereby having important practical application value for solving the rice blast hazard in rice production.
The invention content is as follows:
the invention aims to provide a breeding method of a rice blast resistant two-line sterile line.
Another object of the present invention is to provide a method for breeding a high-quality high-yield two-line hybrid indica rice combination resistant to rice blast.
The purpose of the invention can be realized by the following technical scheme:
a method for breeding a rice blast resistant two-line sterile line comprises the following steps:
1) two-line sterile line parents with excellent comprehensive agronomic characters, good combining ability and no resistance to rice blast are taken as rice blast resistance receptor parents, breeding intermediate material parents containing rice blast resistance Pigm are hybridized with rice blast resistance gene donor parents, and a first hybrid generation F is planted1Then using the parent as recurrent parent, backcrossing for one generation, harvesting BC1F1Seeds;
2) planting BC1F1Detecting rice blast resistance gene Pigm through molecular markers, simultaneously investigating main agronomic characters, selecting sterile single plants with the main agronomic characters like recurrent parents and containing the rice blast resistance gene Pigm, cutting and regenerating, and harvesting BC1F2Seeds and establishing a strain;
3) winter planting of BC in Hainan1F2Continuously detecting rice blast resistance gene Pigm through molecular markers, investigating main agronomic characters in spring, selecting excellent fertile single plants containing the rice blast resistance gene Pigm and the agronomic characters like recurrent parents, and harvesting BC1F3Seeds and establishing a strain;
4) in the season ofYangzhou planting BC1F3Using parent one as reference, integrating MAS detection result, main agronomic character, pedigree breeding, selecting sterile single plant, cutting stubble and regenerating, and harvesting BC1F4Seeds, and continuing to establish a strain;
5) carrying out molecular marker detection on rice blast resistant genes Pigm generation by generation, selecting single plants with main agronomic characters like parent I, and repeating the steps 3) and 4) until BC1F7The plants are sown in stages in the generation, and fertility and main agronomic characters are identified; meanwhile, collecting rice ears naturally infected with rice blast from rice blast re-infected areas in fields, culturing germs, selecting representative virulent monospore strain, eluting rice blast spores after amplification culture, adjusting the concentration to 2 x 105. mL < -1 >, using the strain as an inoculum for identification, and respectively carrying out stable BC treatment on stable BC1F7Carrying out resistance identification on the strains of the seedling blast and the panicle blast; and (3) integrating fertility, main agronomic characters and rice blast resistance identification results, selecting stable strains meeting breeding targets, cutting and regenerating to breed a new rice blast resistant two-line sterile line.
Said parent is preferably the clone 639S; the second parent is preferably Yanggao-resistant No. 6; the Yangyang rice No. 6 is obtained by breeding through the following method: hybridizing Yanggao No. 6 as female parent and Gumei No. 4 as male parent, backcrossing for 3 generations, selfing for 4 generations, and hybridizing from BC1F1Beginning generation, detecting rice blast resistant gene Pigm contained in oryzanol No. 4 by using molecular marker InDel 587 in the whole cultivation process until BC3F5Selecting a strain which is homozygous for the target rice blast resistance gene and has other agronomic characters and yield performances basically consistent with those of Yanggao No. 6, and obtaining the Yanggao No. 6 resistant hybrid indica rice restorer of the two lines with high grain weight and rice blast resistance; the yield level and yield composition factors of Yangdao No. 6 are equivalent to those of the contrast Yangdao No. 6, and the thousand grain weight is stabilized to be more than 30.5 g; the artificial inoculation identification of seedling plague and panicle plague is carried out continuously for three years in perennial reissued rice blast identification of rice blast such as Fujian Jianyang, Jiangxi Jinggang mountain and Anhui Huangshan, Yangdao No. 6 shows rice blast resistance and is obviously superior to Yangdao No. 6.
The breeding method also preferably comprises the following steps of 6): in thatPropagation of new rice blast resistant two-line sterile line (BC) in Hainan1F8) And (3) simultaneously, taking the hybrid rice as a female parent and different restorer lines as male parents to configure hybrid combination, and identifying the grouping advantages of the bred rice blast resistant two-line sterile line.
In the step 1), preferably, the first parent is taken as a female parent and the second parent is taken as a male parent for hybridization; in step 2), taking the parent I as a female parent and F as1The generation is backcrossed as a male parent, and only one generation is backcrossed.
Preferably, collecting rice ears naturally infected with rice blast from rice blast retransmission disease area in step 5), culturing pathogenic bacteria, selecting representative virulent monospore strain, expanding culture, eluting rice blast spore, and adjusting concentration to 2 × 105each.mL-1As an inoculum for identifying rice blast resistance.
The two-line sterile line which is bred according to the method and resists rice blast is preferably Yangxian 6S, and is characterized in that: belongs to a medium indica type thermo-sensitive dual-purpose genic male sterile line; the plants are neat and consistent, the leaf sheaths are colorless, the leaves are green, and the column heads and the lemma tips are colorless; sowing Jiangsu spring, sowing beginning period 102 days, full growth days 138 days, plant height 91cm, main stem and leaf number 15-16; the tillering force is strong, the earring forming rate is high, the average single effective ear is 8-9, the ear length is 23cm, the average number of glumes per ear is 160, the ear neck rate is 15%, and the thousand-grain weight is 25 g; the anther is thin and small, the pollen aborts completely, the pollen-free type is taken as the main material, the sterility degree is 100 percent, the sterile plant rate is 100 percent, the starting temperature of fertility conversion is below 23 ℃, and the sterility is stable. The total exposure rate of the stigma is more than 75%, wherein the bilateral exposure rate is more than 45%, the stigma is large, and the vitality is strong; the pre-noon flower percentage is more than 85 percent, and the outcrossing seed setting percentage is 50 percent; resistance: the rice blast resistant rice blast has the comprehensive index of resisting bacterial leaf blight and rice blast of 3.2; rice quality: the coarseness is 79.0 percent, the whole rice rate is 65.1 percent, the aspect ratio is 3.1, the chalky grain rate is 21.0 percent, the chalky degree is 2.9 percent, the transparency is 2 grade, the gel consistency is 81 mm, and the amylose content is 12.9 percent; the reproducibility is good, and the yield per mu is 265 kg in southern reproduction; the seed production yield is high, the seeds are produced with the restorer line Yanghui 1512, and the average yield per mu is more than 200 kilograms; the advantages of the matching groups are strong, and the yield advantages are obvious and the rice blast resistance is realized when the matching groups are matched with different restorer lines.
A breeding method of a rice blast resistance high-quality high-yield two-line hybrid indica type rice combination comprises the steps of configuring a hybrid combination by taking a strong-advantage and high-quality restorer line as a male parent on the basis of breeding a rice blast resistance two-line sterile line, identifying the panicle blast resistance of the hybrid combination at the booting stage on the premise of ensuring that the yield is higher and the yield is increased by No. 4 hybrid progeny, and comprehensively evaluating the rice blast resistance of the configured hybrid combination by combining the rice blast disease nursery resistance identification result; and harvesting rice of hybrid progeny of all the hybrid combinations, and detecting the rice quality of all the hybrid combinations according to the national standard of high-quality rice to obtain the new rice combination of rice blast-resistant high-quality high-yield two-line hybrid indica rice.
The two-line rice blast resistant sterile line is preferably obtained by breeding by the method; further preferably yangxian 6S.
The strong-superiority and high-quality recovery system preferably has Yang Hui 1512.
According to the breeding method, the two-line sterile line Yanghong 6S resistant to rice blast and the restorer line Yanghui 1512 are matched to breed the new Yanghuangyou 612 of the two-line hybrid indica rice with high quality and high yield resistant to rice blast, and the breeding method is characterized in that: yangliangyou 612 belongs to indica type two-line hybrid rice variety; planting middle-season rice in the middle and lower reaches of Yangtze river, wherein the whole growth period is 138.0 days, and the length is 2.4 days longer than that of the No. four rice with Fengliang; the average height of the plant is 129.3cm, the ear length is 25.7cm, the number of effective ears per mu is 17.1 ten thousand, the total number of the ears per mu is 191.8, the seed setting rate is more than 82.6 percent, and the weight of thousand seeds is 26.0 g; resistance: the comprehensive resistance indexes of the rice blast are 3.1 and 2.6 respectively in two years, and the highest neck blast loss rate is grade 5; grade 7 of bacterial leaf blight; brown planthopper grade 9; the heat resistance in the heading stage is grade 3; main indexes of rice quality are as follows: the whole rice rate is 61.5%, the length-width ratio is 3.1, the chalky grain rate is 20%, the chalky degree is 3.1%, the gel consistency is 66mm, the amylose content is 19.0%, the standard reaches the high-quality grade 3 of the national high-quality rice standard, the standard of the Ministry of agriculture, the high-quality grade III of the edible rice variety quality standard, and the like; in 2016, the average yield per mu of the green channel region test of middle and lower indica late maturing groups in Yangtze river is 646.35 kg, and the yield is increased by 6.52 percent compared with the yield of the control Fengliangyou No. four; the test is continued in 2017, the average yield per mu is 639.20 kg, and the yield is increased by 5.09 percent compared with the yield of the control Fengliang No. four, and the extremely obvious level is achieved; the yield of the two-year district test is 642.78 kg per hectare on average, and the yield is increased by 5.80 percent compared with the yield of the control Fengliou No. four; the production test in 2017 shows that the average yield per mu of 659.04 kg is increased by 10.11 percent compared with the control Fengliouyou No. four, and the extremely obvious level is achieved.
The invention has the beneficial effects that:
the invention discloses a breeding method of a rice blast resistant high-quality high-yield two-line hybrid indica rice combination. The method relates to a breeding intermediate material with excellent comprehensive properties, which is the rice blast resistance gene Pigm selected by molecular Marker Assisted Selection (MAS), and the disease resistance gene donor Yangyang No. 6, so that the undesirable linkage drag carried by the original disease resistance gene donor is avoided, and the breeding time is shortened; in the lower generation (BC)1F1) Starting main agronomic character selection, and selecting a single plant with agronomic characters close to the recurrent parent (Longke 638S); advanced generation (BC)1F7) Carrying out fertility and main agronomic character investigation and identification on the stable strain, and carrying out seedling blast and panicle blast rice blast resistance identification on the stable strain; in the whole process, sterile single plants are selected for cutting and regenerating in Yangzhou in the positive season, fertile single plant breeding seeds are selected in Hainan in spring, and the seeds undergo south breeding and north breeding; finally, a new rice blast resistant two-line sterile line is bred by integrating the identification results of fertility, main agronomic characters and rice blast resistance; the sterile line and the high-advantage and high-quality restorer line bred by the method are matched, and the new rice blast-resistant high-quality and high-yield two-line hybrid indica rice combination is bred by carrying out yield advantage identification, rice quality detection, panicle blast inoculation identification and rice blast disease garden resistance identification on the hybrid combination. The hybrid combination has the following advantages: (1) the rice blast resistant gene Pigm is expressed in the combination of the bred two-line sterile line and the hybrid rice through molecular marker assisted selection and genetic improvement, so that the rice blast resistant gene Pigm is resistant to rice blast and beneficial to protecting the intellectual property of varieties. (2) The bred two-line sterile line not only contains rice blast resistance gene Pigm, but also retains the excellent agronomic characters of the receptor parent, and the hybrid combination prepared by using the two-line sterile line as the female parent has high and stable yield. (3) The new combination of two-line hybrid rice bred by taking a high-superiority and high-quality restoring line as a male parent and matching with a newly bred two-line sterile line containing the rice blast resistance gene Pigm not only has the advantages of rice blast resistance and large yield potential, but also has good rice quality which can reach the standard of national high-quality rice.
Description of the drawings:
FIG. 1: the spectrum of the anti-rice blast two-line sterile line Yangxian 6S breeding line is shown schematically
FIG. 2: the schematic diagram of the breeding process of the high-quality high-yield two-line hybrid indica rice variety Yangliangyou 612 capable of resisting rice blast of the invention
The specific implementation mode is as follows:
the breeding method of the rice blast-resistant high-quality high-yield two-line hybrid indica rice combination is further described by combining the attached drawings.
Example 1
1. Breeding materials:
1) the mother plant was 638S of Longke. Longke 638S is a two-line sterile line cultivated by the institute of science and technology in the Yahua variety of Hunan province. The variety has been applied and authorized to the new plant variety protection office of Ministry of agriculture, and the variety right number: CNA20090950.7, available from the Asahua institute of science, Hunan. The variety is a two-line sterile line widely used in recent years in China, and the prepared hybrid rice has the advantages of high yield, high quality, wide adaptability and the like.
2) Yangyang rice No. 6 (Yangyang rice No. 6 x Valley No. 4, BC)3F5) Used as a male parent. Wherein Yanggao No. 6 is cultivated by agricultural science research institute in Rixia river region of Jiangsu. The variety has been applied and authorized to the new plant variety protection office of Ministry of agriculture, and the variety right number: CAN20000091.8, available from Jiangsu gold land species industry Co. The variety is a backbone restoring line of two-line hybrid rice in China, and the prepared hybrid rice has the advantages of high yield, high quality, wide adaptability and the like. The oryza sativa No. 4 is a local indica rice variety in China, carries a rice blast resistance gene Pigm, and is a good germplasm for expressing broad-spectrum and lasting rice blast resistance in China. See in detail: deng Y, ZHai K, Xie Z, Yang D, Zhu X, Liu J, Wang X, Qin P, Yang Y, Zhang G, Li Q, Zhang J, Wu S, Milazzo J, Mao B, Wang E, Xie H, Tharreau D, He Z. epigenetic regulation of anti-inflammatory receptors control blast resistance with yield band. science,2017,355: 1-4. Yanggao No. 6 is the agricultural scientific research in the region of Ri river of Jiangsu, so Yanggao No. 6 is used as female parent, Gumei No. 4 is used as male parent to make hybridization, then Yanggao No. 6 is used as recurrent parent, and is backcrossed for 3 generations, then is self-crossed for 4 generations, and is made into BC1F1Beginning generation, detecting rice blast resistant gene Pigm contained in oryzanol No. 4 by using molecular marker InDel 587 in the whole cultivation process until BC3F5And (3) selecting a strain line which is homozygous for the target rice blast resistance gene and has other agronomic characters and yield performances basically consistent with those of Yanggao No. 6, and obtaining the Yanggao-resistant high-grain-weight two-line hybrid indica rice restorer line. The yield level and yield composition factors of Yangdao No. 6 are equivalent to those of the contrast Yangdao No. 6, and the thousand grain weight is stabilized to be more than 30.5 g; the artificial inoculation identification of seedling plague and panicle plague is carried out continuously for three years in perennial reissued rice blast identification areas of rice blast such as Fujian Jianyang, Jiangxi Jinggang mountain and Anhui Huangshan, Yangdao No. 6 shows rice blast resistance and is obviously superior to the control Yangdao No. 6.
2. The breeding of the rice blast resistant two-line sterile line is shown as the attached figure 1:
1) the two-line sterile line clone 638S with excellent comprehensive agronomic characters and good combining ability but without rice blast resistance is used as a female parent, and a breeding intermediate material containing rice blast resistance gene Pigm is used for resisting Yangdao No. 6 (Yangdao No. 6 multiplied by oryzanol No. 4, BC)3F5) Hybridizing as male parent, planting the first filial generation F1Then taking 638S of Longke as recurrent parent, backcrossing for one generation, and harvesting BC1F1Seeds;
2) planting BC1F140 groups of plants are detected by a molecular marker to detect rice blast resistance gene Pigm, the detection marker is InDel 587 (the primer sequences are 5'-ACTTGCTGGGAGAAGGATT-3' (SEQ ID NO.1) and 5'-AGTTCGTACTTTTCAGGCT-3' (SEQ ID NO.2)), main agronomic characters are considered at the same time, 18 sterile single plants which have the main agronomic characters like recurrent parents and contain the rice blast resistance gene Pigm are selected, stubbles are regenerated, and BC is harvested1F2Seeds and establishing a strain;
3) winter planting of BC in Hainan1F2Continuously detecting rice blast resistance gene Pigm through molecular markers, investigating main agronomic characters in spring, selecting excellent fertile single plants 12 containing the rice blast resistance gene Pigm and the agronomic characters like 638S of Croton, and harvesting BC1F3Seeds and establishing a strain;
4) sowing BC in Yangzhou in season1F3To long runThe family 638S is used as a control, MAS detection results are integrated, main agronomic characters are inspected, genealogical breeding is carried out, 9 sterile single plants are selected, stubble regeneration is carried out, and BC is harvested1F4Seeds, and continuing to establish a strain;
5) carrying out molecular marker detection on rice blast resistant gene Pigm generation by generation, selecting single plants with main agronomic characters like 638S of Croton, repeating the steps 3) and 4) until BC1F7The plants are sown in stages in the generation, and fertility and main agronomic characters are identified; meanwhile, rice ears naturally infected with rice blast are collected from rice blast retransmission disease areas such as Guangdong, Hubei, Hainan, Zhejiang and Jiangsu, germs are cultured, 5 representative virulent monospore strains GD18-3(B1, the pathogenic rate is 65.6%), ES9-5-1(B15, the pathogenic rate is 75.0%), hnn1-1(C1, the pathogenic rate is 59.4%), ZJ3-4(A15, the pathogenic rate is 63.3%) and XK8-4-4(G1, the pathogenic rate is 37.5%) are selected, rice blast spores are eluted after expanded culture, the concentration is adjusted to be 2 x 105. mL-1, and the obtained rice blast spores are used as inoculants for identification and are used for respectively resisting stable BC (rice blast)1F7Carrying out resistance identification on the strains of the seedling blast and the panicle blast rice blast; the evaluation results of fertility, main agronomic characters and rice blast resistance are integrated, the overall character of the strain S score 6 is excellent, the breeding target is met, and the strain S score can be used as a new rice blast resistant two-line sterile line.
6) Propagation of lines S-score 6 (BC) in Hainan1F8) And the hybrid combination is configured by using the Yangxian 6S as a name and using different restorer lines as male parents, and the mating advantages of the bred two-line sterile line Yangxian 6S for resisting the rice blast are identified.
7) The rice blast resistant two-line sterile line Yangxian 6S bred according to the method has the following sterility and characteristic characteristics:
sterility: the Yangzuan 6S is identified under natural conditions of Yangzhou, the sterile stable period is between 7 months, 20 days and 8 months, 30 days, the anther is thin and small, the pollen aborts thoroughly, the pollen-free type is taken as the main, the sterility degree is 100 percent, the sterile plant rate is 100 percent, the bagging selfing maturing rate is 0, and the hybrid male sterile line belongs to a dual-purpose nuclear sterile line with lower starting point temperature. 2017 evaluation of phytotron of the China Rice research institute: yangxian 6S is a thermo-sensitive dual-purpose genic male sterile line, the starting temperature of fertility conversion is below 23 ℃, and the national standard is met.
Agronomic traits: the Yangxian 6S plants are neat and consistent, the leaf sheaths are colorless, the leaves are green, and the column heads and the lemma tips are colorless. Sowing is carried out in 5 months and 10 days in Yangzhou, the initial period of sowing is about 102 days, the total growth days is about 138 days, the plant height is about 91cm, and the number of main stems and leaves is 15-16. The tillering force is strong, the earring forming rate is high, the average single effective ear is 8-9, the ear length is about 23cm, the average number of glumes per ear is about 160, the ear neck covering rate is about 15%, and the thousand-grain weight is about 25 g.
Flowering habit: the flowering period of single spike of Yangxiang 6S is about 7-8 days, and the flowering period of single plant is about 10 days. 3-6 days after heading, the flowering heads account for about 80% of the total flowering quantity, the opening angle of glumes is about 30 degrees, and the variation of the number of single glumes from opening to closing is 80-120 minutes. In fine weather conditions, flowers begin at 8:30 am, the peak of daily flowering is at 9:30-12:00, the pre-noon flowering rate is more than 85%, the total stigma exposure is more than 75%, and the double exposure rate is more than 45%. Under the condition of bagging artificial pollination, the outcrossing seed setting rate can reach about 70 percent; in the seed production field, the outcrossing seed setting rate can reach about 50 percent. Is sensitive to "nine two 0".
Rice quality: according to the detection: the yield of the indica 6S is 79.0 percent, the whole rice rate is 65.1 percent, the chalky grain rate is 21.0 percent, the chalky degree is 2.9 percent, the length-width ratio is 3.1, the amylose content is 12.9 percent, the gel consistency is 8mm, and the transparency is 2 grade.
Resistance: inoculation and identification, wherein bacterial leaf blight is resisted in the indica 6S; 2011-2013, the natural induction identification of perennial rice blast resending areas such as Enshi in Hubei, Jiangxi Tuchuan, Anhui Jinzhai, Hangzhou in Fujian and the like has a comprehensive index below 5; after being mixed, the strong pathotype representative strains of rice blast in 5 different ecological areas of Guangdong, Hubei, Hainan, Zhejiang and Jiangsu are artificially inoculated and identified for two years, the seedling blast can resist the rice blast, and the panicle blast can resist the rice blast with the grade of 3.
Propagation and seed production characteristics: the reproductive performance is better, the breeding is carried out in Hainan in 2013 spring, and the yield per mu is 263.5 kg; the seed production yield is high, the combination seed production of the Yangxi 6S/Yanghui 1512 and the like is carried out in Yangzhou in 2013-2014 for two continuous years, and the average yield per mu is more than 200 kg.
The matching advantages are strong: the Yangxi 6S is matched with different restorer lines, the yield advantage is obvious, all combinations are increased compared with Fengliouyou No. four, and the rice blast resistance is realized.
3. The new rice blast-resistant high-quality high-yield two-line hybrid indica rice is bred with Yangliangyou 612, as shown in the attached figure 2:
1) the new Yangliang hybrid rice 612 with rice blast resistance, high quality and high yield is bred by taking Yanghong 6S as a female parent and Yanghui 1512 as a male parent. Performing advantage identification in Yangzhou in the quarter of 2013, wherein the performance is outstanding, rice blast germs are inoculated in the booting stage, the panicle blast is about 3.0, and the rice blast is resisted; 2014-2015, and the rice blast perennial reissued areas such as Enshi in Hubei, Jiangxi Tuchuan, Anhui Jinzhai, Hangzhou in Fujian and the like naturally induce and identify, wherein the comprehensive indexes of the rice blast are all about 3.0, and the rice blast has excellent performance, abundance, resistance and wide adaptability. 2016-plus 2017 participates in the green channel region test of the late maturing group of the middle and downstream indica in the Yangtze river of the southern rice region of China, 2017 participates in the green channel production test of the late maturing group of the middle and downstream indica in the Yangtze river of the southern rice region of China, and large-area demonstration planting is carried out at the same time. Approved by the national crop variety approval committee in 2018.
2) Characteristic characteristics of Yangliangyou 612
High and stable yield, large yield potential:
in 2016-2017 experiments of participating in green channel region experiments of late-maturing middle indica rice groups in the middle and lower reaches of Yangtze river in the southern rice region of China, the average yield per mu of 646.35 kg in 2016 is increased by 6.52 percent compared with the yield of the Fengliou No. four, which reaches an extremely obvious level; in 2017, the average yield per mu is 639.20 kg, and the yield is increased by 5.09 percent compared with the yield of a control Fengliangyou No. four, and the extremely obvious level is achieved; the yield of the district test in two years is 642.78 kg per hectare on average, and the yield is increased by 5.80 percent compared with the yield of the control Fengliangyou No. four; in the production test in 2017, the average yield per mu of 659.04 kg is increased by 10.11 percent compared with the yield per mu of Fenglian No. four, and the yield per mu reaches an extremely obvious level.
The quality is good, and the commodity is good:
the main indexes of the rice quality are as follows: the rice has the advantages of 61.5% of whole rice rate, 3.1% of aspect ratio, 20% of chalky grain rate, 3.1% of chalky degree, 66mm of gel consistency and 19.0% of amylose content, and reaches the third grade of high quality of the national high-quality rice standard, the third grade of high quality of the edible rice variety quality standard of Ministry of agriculture, and the like.
And (3) rice blast resistance:
the comprehensive rice blast resistance indexes are respectively 3.1 and 2.6 in two years, and the highest neck blast loss rate is grade 5.
The agronomic characters are excellent, and the applicability is wide:
the seedlings are short and strong, the leaf sheaths are short, thick and green, the leaves are straight, the leaf color is dark green, and the tillering performance is strong; the plant type of the adult plant is compact, the average plant height is 129.3cm, the upper three functional leaves are straight, the light receiving posture is good, the sword leaves lift up during maturity, and the green stems are yellow and mature; the tillering performance is strong, the spike shape is large, the spike length is 25.7cm, the effective spike per mu is 17.1 ten thousand, the total number of the total grains per spike is 191.8, the seed setting rate is more than 82.6 percent, and the thousand-grain weight is 26.0 g; the full growth period is 138.0 days, which is 2.4 days longer than the control Fengliangyou No. four, and belongs to the type of the two-line late-maturing hybrid rice of the middle indica type.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (5)
1. A method for breeding a rice blast resistant two-line sterile line is characterized by comprising the following steps:
1) uses two-line sterile line parents which have excellent comprehensive agronomic characters and good combining ability but do not resist rice blast resistance as rice blast resistance gene receptor parents to contain rice blast resistance genesPigmThe breeding intermediate material parent is crossed as the rice blast resistant gene donor parent, and the first hybrid generation F is planted1Then using the parent as recurrent parent, backcrossing for one generation, harvesting BC1F1Seeds;
2) planting BC1F1Population, detection of rice blast resistance genes by molecular markersPigmSimultaneously, the main agronomic characters are inspected, the main agronomic characters are selected like recurrent parents and contain rice blast resistance genesPigmThe sterile single plant is cut and regenerated, and BC is harvested1F2Seeds and establishing a strain;
3) winter planting of BC in Hainan1F2Continuing to detect the rice blast resistance gene by molecular markersPigmInspecting main agronomic characters in spring and selecting rice blast resistance genePigmAnd the agronomic character is like the superior fertile single plant of the parent IHarvesting of BC1F3Seeds and establishing a strain;
4) planting BC in Yangzhou in normal season1F3Using parent-one as contrast, integrating molecular marker auxiliary selection MAS detection result, investigating main agronomic character, genealogical breeding, selecting sterile single plant, cutting stubble and regenerating, and harvesting BC1F4Seeds, and continuing to establish a strain;
5) generation by generation molecular marker detection of rice blast resistance genePigmSelecting single plant with main agronomic characters like parent I, repeating steps 3) and 4) until BC1F7The plants are sown in stages in the generation, and fertility and main agronomic characters are identified; simultaneous respectively stable BC1F7Performing resistance identification on the strains of the Miao plague and the ear plague; synthesizing fertility, main agronomic characters and rice blast resistance identification results, selecting stable strains meeting breeding targets, cutting and regenerating to breed a new rice blast resistant two-line sterile line;
wherein the first parent is Longke 638S; the second parent is Yangyang rice No. 6; the Yangyang rice No. 6 is obtained by breeding through the following method: hybridizing Yanggao No. 6 as female parent and Gumei No. 4 as male parent, backcrossing for 3 generations, selfing for 4 generations, and hybridizing from BC1F1Beginning with generation, detecting the rice blast resistance gene contained in the oryzanol No. 4 by using a molecular marker InDel 587 in the whole cultivation processPigmUp to BC3F5Selecting a strain which is homozygous for the target rice blast resistance gene and has other agronomic characters and yield performances basically consistent with those of Yanggao No. 6, and obtaining the Yanggao No. 6 resistant hybrid indica rice restorer of the two lines with high grain weight and rice blast resistance; the yield level and yield composition factors of Yangdao No. 6 are equivalent to those of the contrast Yangdao No. 6, and the thousand grain weight is stabilized to be more than 30.5 g; the artificial inoculation identification of the seedling plague and the panicle plague is continuously carried out in perennial severe rice blast areas such as Fujian Jianyang, Jiangxi Jinggang mountain, Anhui Huangshan and the like for three years, and the resistance to Yangdao No. 6 shows resistance to rice blast and is obviously superior to that of Yangdao No. 6.
2. The breeding method according to claim 1, characterized in that the breeding method further comprises step 6): the new rice blast resistant two-line sterile line seeds are propagated in Hainan, meanwhile, the seeds are used as female parents, different restoring lines are used as male parents, and hybridization combination is configured to identify the matching advantages of the bred rice blast resistant two-line sterile lines.
3. The breeding method according to claim 1, wherein in step 1), the first parent is used as female parent and the second parent is used as male parent for hybridization; in step 1), taking the parent I as a female parent and F as a female parent1The generation is backcrossed as a male parent, and only backcross the first generation.
4. The breeding method according to claim 1, characterized in that: collecting rice ears naturally infected with rice blast from rice blast retransmission disease area, culturing pathogenic bacteria, selecting representative virulent monospore strain, expanding culture, eluting rice blast spore, and adjusting concentration to 2 × 105each.mL-1As an inoculum for identifying rice blast resistance.
5. A breeding method of a rice blast resistance high-quality high-yield two-line hybrid indica type rice combination is characterized by comprising the steps of configuring a hybrid combination by taking a strong-advantage and high-quality restorer line as a male parent on the basis of breeding a rice blast resistance two-line sterile line, identifying the panicle blast resistance of the hybrid combination at the booting stage on the premise of ensuring the yield to be richer and increasing the yield of No. 4 hybrid progeny, and comprehensively evaluating the rice blast resistance of the configured hybrid combination by combining the rice blast disease nursery resistance identification result; harvesting rice of hybrid progeny of all the hybrid combinations, and detecting the rice quality of all the hybrid combinations according to the national standard of 'high-quality rice', thereby obtaining a new rice combination of two-line hybrid indica rice with high quality and high yield and capable of resisting rice blast; the two-line sterile line resistant to rice blast is obtained by breeding by the method of claim 1 or 2.
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| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
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