CN111903500B - Breeding method of fragrant rice blast resistant restorer cinnamon 5886 with high harvest index - Google Patents
Breeding method of fragrant rice blast resistant restorer cinnamon 5886 with high harvest index Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention provides a breeding method of fragrant rice blast resistant restorer of cinnamon 5886 with high harvest index, which comprises the steps of hybridizing the cinnamon 582 with high harvest index as a female parent and the eight cinnamon of a fragrant rice variety as a male parent, performing backcross for 2 times by using the cinnamon 582 as a recurrent parent to construct a segregation population, adopting molecular marker assisted breeding, and polymerizing fragrance genesfgrRice blast resistance genepita、pi1、pi5The fragrant rice blast resistant restorer line rice variety with high harvest index is bred through conventional pedigree breeding, harvest index identification and rice blast resistance identification, and is named as Gui 5886.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a breeding method of a fragrant rice blast resistant restorer namely osmanthus fragrans 5886 with a high harvest index.
Background
The rice harvest index is the ratio of the rice yield to the total biological yield of the aboveground parts. The rice harvest index is an important embodiment of the rice production efficiency, and under the condition of certain total biomass, the improvement of the harvest index is an important way for increasing the yield. The harvest index increases by 0.1 percentage point per hectare per season, meaning that 1500kg of yield can be increased per hectare per season. The improvement of the harvest index of the rice variety has important significance for improving the yield per unit of rice and guaranteeing the national grain safety.
The rice blast harm causes the annual output loss of global rice by about 18 percent, the rice areas in the south and north of China are harmed by different degrees every year, the yield is reduced by 10 to 20 percent generally in serious disease areas in popular years, the serious rate reaches 40 to 50 percent, and local field blocks or even grains are not harvested. The cultivation of broad-spectrum rice blast resistant rice varieties by using rice blast resistant genes is the most economical and effective method for preventing and treating the rice blast. However, in recent years, the rice blast resistant varieties bred in various places in China are low in proportion, and most varieties are still susceptible varieties. The prevention and control of rice blast diseases in China are still severe, and the breeding of rice blast resistance needs to be further strengthened.
Therefore, the breeding of new rice varieties with high harvest index and rice blast resistance plays an important role in high and stable yield of rice.
Disclosure of Invention
Aiming at the defect that the rice blast-resistant variety ratio of rice bred in multiple places of China is low, the invention provides a breeding method of a fragrant rice blast-resistant restorer cinnamon 5886 with high harvest index, and rice plant lines with high harvest index and rice blast resistance are bred in an auxiliary mode through molecular markers.
The invention is realized by the following technical scheme:
a breeding method of fragrant rice blast resistant restorer cinnamon 5886 with high harvest index comprises the following steps:
(1) taking cinnamon 582 as a female parent and Bagui Xiang as a male parent, and hybridizing to obtain an F1 generation;
the cinnamon 582 containspitaA gene; the eight-cinnamon-leaf containsfgr、pi1、pi5、pitaA gene;
saidfgrIs a gene of the fragrance, and the gene is a gene of the fragrance,pi1、pi5、pitais a rice blast resistance gene;
(2) planting F1 generation, backcrossing with Gui582 as recurrent parent, and harvesting BC1F1Seeds;
(3) planting BC1F1Seed, extraction of BC1F1DNA of the generation line, adopting molecular marker to assist, selecting the DNA containing the target genefgr、pita、pi1、pi5Taking the single plant as a female parent and taking the cinnamon 582 as a male parent for backcross, and harvesting the BC2F1Seeds;
(4) planting BC2F1Seeds, selection of seeds containing target gene by molecular marker assistancefgr、pita、pi1、pi5The single plants are mixed and harvested to obtain BC2F2Seeds;
(5) planting BC2F2The seeds are selected to have strong rice blast resistance, good plant leaf type, more big ears, moderate growth period and high setting rateObtaining BC according to single plant of strain with excellent comprehensive character2F3Seeds; planting for two successive generations, and selecting and tracking target gene with the aid of molecular markerfgr、pita、pi1、pi5Directional preferential breeding is carried out, the seeds are gradually eliminated, and seeds with strong resistance are harvested according to single plants;
(6) planting to obtain seeds with strong resistance, and adopting molecular marker to assist selection and tracking of target genefgr、pita、pi1、pi5Directionally selecting strains with high plant height of 100-115 cm, large ears, high maturing rate, moderate growth period, good maturity color and other excellent agronomic characters, identifying a harvest index of 0.50-0.60, and obtaining a target genefgr、pita、pi1、pi5Homozygous, aromatic flavor, thick and strong stem, moderate growth period, good mature color and strong heterosis of matched hybrids, namely a target plant line named as Gui 5886.
The invention carries out genetic improvement from hybrid rice recovery line materials, takes cinnamon 582 as a female parent and osmanthus fragrans as a male parent for hybridization, then takes the cinnamon 582 as a recurrent parent for carrying out 2 times of backcross to construct a segregation population, adopts molecular Marker Assisted (MAS) breeding and polymerizes aroma genesfgrRice blast resistance genepita、pi1、pi5The fragrant rice blast resistant restorer line rice variety with high harvest index is bred through conventional pedigree breeding, harvest index identification and rice blast resistance identification, and is named as Gui 5886.
Further, the harvest index of the cinnamon 582 is 0.58.
Further, the strain with large spike and large grain in the step (6) is 160-200 per spike.
Further, the fructification rate in the step (6) is more than 80%.
The invention has the beneficial effects that:
the rice variety bred by the invention has polymerized fragrance genefgrRice blast resistance genepita、pi1、pi5The rice blast resistance is strong, the plant leaf type is good, the ears are large, the growth period is moderate, the setting rate is high, the harvest index is high and other excellent comprehensive properties are realized.
Drawings
FIG. 1 is a flow chart of breeding of cinnamon 5886 of example 1.
Detailed Description
The invention will be further explained with reference to the drawings.
Example 1
A breeding method of a fragrant rice blast resistant restorer of rice blast 5886 with high harvest index is shown in figure 1, and specifically comprises the following steps:
(1) in the early season of 2012, taking cinnamon 582 as a female parent and eight cinnamon as a male parent for hybridization, and harvesting F1 hybrid seeds;
(2) planting F1 material in late season of 2012, determining true hybrid single plant by molecular marker detection, backcrossing with true hybrid single plant as female parent and Gui 582 as male parent, and harvesting BC1F1Seeds;
(3) planting BC in early season of 20131F1Material for selecting target-containing genes by molecular marker-assisted (MAS) selectionfgr、pita、pi1、pi5Taking the single plant as a female parent and taking the cinnamon 582 as a male parent for backcross, and harvesting the BC2F1Seeds;
the target genefgr、pita、pi1、pi5The primer information of (A) is shown in Table 1;
(4) planting BC in late season of 20132F1Materials containing target genes selected by MASfgr、pita、pi1、pi515 single plants with good comprehensive properties such as good plant leaf morphology, good mature color, strong advantages and the like are mixed and harvested;
(5) planting BC in early 20142F26000 single plants of the material are screened in a Luoxixiang rice blast area of Guangxi Jinxiu rice, single plants with good plant leaf morphology, strong rice blast resistance, more ears and large grains, high maturing rate, moderate growth period, good mature color and other excellent agronomic characters are directionally selected, and 56 target single plants are harvested;
(6) planting BC in 2014 to 20152F3～BC2F5Materials, carrying out rice blast resistance identification in the Guangxi Jinxiuoxiang rice blast area, tracking target genes by MAS, directionally and preferentially breeding, gradually eliminating, and obtaining BC in late season of 20152F525 target single plants;
(7) planting BC from 2016 early season to 2018 late season2F6～BC2F11The material is used in a test field of rice institute of Guangxi academy of agricultural sciences, MAS tracks target genes, plants with the plant height of 100-115 cm, large panicle (160-200 grains/panicle), high seed setting rate (more than 80%), moderate growth period, good mature color and other excellent agronomic characters are selected in an oriented mode to 2018 early season (BC)2F10Generation), 15 target strains are obtained, 5 strains are obtained between 0.50 and 0.60 of the harvest index through the harvest index identification of 15 strains, wherein the strain with the area code of 88 has excellent comprehensive properties, and the target genefgr、pita、pi1、pi5Homozygous, harvest index of 0.56, aromatic flavor, thick and strong stem, plant height of 108.6cm, average 180 grains per spike, grain length of 9.60mm, grain width of 2.32mm, thousand grain weight of 23.2g, moderate growth period, good maturity color and strong heterosis of matched hybrids, and is named as Gui 5886;
(8) in the late season of 2018, carrying out rice blast resistance re-identification on the plant line with the area code of 88, wherein in the Guangxi Jinxiuoxiang rice blast area, the seedling leaf blast is grade 1, and the panicle blast is grade 1; and (4) identifying by artificial inoculation, wherein the seedling leaf blast is grade 1, and the panicle blast is grade 1-3.
Step (3) target Genefgr、pita、pi1、pi5The molecular marker information of (2) is shown in Table 1.
TABLE 1 genes of interestfgr、pita、pi1、pi5Primer information for molecular detection
The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the scope of the present invention is defined by the claims. Various modifications and equivalents may be made thereto by those skilled in the art within the spirit and scope of the present invention, and such modifications and equivalents should be considered as falling within the scope of the present invention.
Claims (4)
1. A breeding method of a fragrant rice blast resistant restoring line osmanthus 5886 with a high harvest index is characterized by comprising the following steps:
(1) taking cinnamon 582 as a female parent and Bagui Xiang as a male parent, and hybridizing to obtain an F1 generation;
the cinnamon 582 containspitaA gene; the eight-cinnamon-leaf containsfgr、pi1、pi5、pitaA gene;
saidfgrIs a gene of the fragrance, and the gene is a gene of the fragrance,pi1、pi5、pitais a rice blast resistance gene;
(2) planting F1 generation, backcrossing with Gui582 as recurrent parent, and harvesting BC1F1Seeds;
(3) planting BC1F1Seed, extraction of BC1F1DNA of the generation line, adopting molecular marker to assist, selecting the DNA containing the target genefgr、pita、pi1、pi5Taking the single plant as a female parent and taking the cinnamon 582 as a male parent for backcross, and harvesting the BC2F1Seeds;
(4) planting BC2F1Seeds, selection of seeds containing target gene by molecular marker assistancefgr、pita、pi1、pi5The single plants are mixed and harvested to obtain BC2F2Seeds;
(5) planting BC2F2Selecting seeds, selecting a plant line with strong rice blast resistance, good plant leaf type, large panicle, moderate growth period and high maturing rate and excellent comprehensive characters, and harvesting BC according to a single plant2F3Seeds; planting for two successive generations, and selecting and tracking target gene with the aid of molecular markerfgr、pita、pi1、pi5Directional preferential breeding is carried out, the seeds are gradually eliminated, and seeds with strong resistance are harvested according to single plants;
(6) planting to obtain seeds with strong resistance, and adopting molecular marker to assist selection and tracking of target genefgr、pita、pi1、pi5Directionally selecting strains with high plant height of 100-115 cm, large ears, high seed setting rate, moderate growth period and good maturity and color and excellent agronomic characters, and identifying harvest indexes, wherein the harvest indexes are 0.50-0.60 and target genesfgr、pita、pi1、pi5Homozygous, aromatic flavor, thick and strong stem, moderate growth period, good mature color and strong heterosis of matched hybrids, namely the target plant lineNamed as Gui 5886;
the markfgrThe primer sequences of the genes are as follows:
FgrF:TACCCCATCAATGGAAATGA；
FgrR:AACCATAGGAGCAGCTGAA；
the markpi1The primer sequences of the genes are as follows:
pi1F:GTGCTGCTGTGGCTAGTTTG；
pi1R:AGTCCCCGCTCAATTTTTCT；
the markpi5The primer sequences of the genes are as follows:
pi5F:ATAGATCATGCGCCCTCTT；
pi5R:TCATACCCCATTCGGTCAT；
the markpitaThe primer sequences of the genes are as follows:
PitaF:CTCCCTTGTTCGGTCAAAA；
YL155：AGCAGGTTATAAGCTAGG；
PitaR:CCACATTGATGAAGCCCAT。
2. the breeding method of the high-harvest-index aromatic rice blast-resistant restorer line cinnamon 5886 according to claim 1, characterized in that: the harvest index of the cinnamon 582 is 0.58.
3. The breeding method of the high-harvest-index aromatic rice blast-resistant restorer line cinnamon 5886 according to claim 1, characterized in that: the strain with large ears and large particles in the step (6) is 160-200 particles/ear.
4. The breeding method of the high-harvest-index aromatic rice blast-resistant restorer line cinnamon 5886 according to claim 1, characterized in that: the setting rate in the step (6) is more than 80%.
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| CN113207682B (en) * | 2021-06-02 | 2022-03-29 | 广西壮族自治区农业科学院 | Rice breeding method for strengthening polygene polymerization and genetic background stability of high-yield, high-quality and biotic and abiotic stress resistant rice |
| CN114041416A (en) * | 2021-06-28 | 2022-02-15 | 广东省农业科学院水稻研究所 | Breeding method of disease-resistant fragrant Guangdong silk seedling rice variety |
| CN115735763A (en) * | 2022-11-08 | 2023-03-07 | 宜春市科学院(江西富硒产业研究院) | Breeding method of conventional variety of long-grain fragrant high-quality rice |
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