CN115735763A - Breeding method of conventional variety of long-grain fragrant high-quality rice - Google Patents
Breeding method of conventional variety of long-grain fragrant high-quality rice Download PDFInfo
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- CN115735763A CN115735763A CN202211393054.XA CN202211393054A CN115735763A CN 115735763 A CN115735763 A CN 115735763A CN 202211393054 A CN202211393054 A CN 202211393054A CN 115735763 A CN115735763 A CN 115735763A
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Abstract
The invention belongs to the technical field of rice breeding and discloses a breeding method of a conventional variety of long grain fragrance type high-quality rice. Hybridizing the primary female parent and the primary male parent to obtain F1 generation seeds; planting to obtain F2 generation rice plants; extracting DNA of long-grained rice plants with excellent performance based on the selected field characters; by utilizing a molecular marker assisted selection technology, carrying out close interlocking molecular marker detection on the extracted DNA aroma gene and the Pigm rice blast resistance gene, screening rice plants which simultaneously have the aroma gene and the Pigm rice blast resistance gene and have long grain characters as a second-generation female parent, and carrying out backcross with a primary male parent to obtain BC1F 2-generation rice; multiple selfing is carried out on the rice of BC1F3 generation, and a high-quality rice conventional variety with long-grain odor type and rice blast resistance is bred by combining the auxiliary selection of molecular markers of the fragrance gene and the Pigm rice blast resistance gene. The invention breeds the high-quality conventional rice variety which has slender grains, rice blast resistance, fragrant type and good palatability.
Description
Technical Field
The invention belongs to the technical field of rice breeding, and relates to a breeding method of a conventional variety of long grain fragrance type high-quality rice.
Background
At present, the food is the national security important foundation for transporting the people's life in the food affairs state. The key point of grain delivery safety lies in the fact that grain storage is performed in the ground and grain storage is performed in the technical strategy, and seeds are 'chips' of agriculture, so that high-quality and high-yield varieties are the basis for ensuring grain safety.
In recent years, with the increasing living standard of people, people's demand for quality of rice taste is increasing, and research on rice breeding for improving quality of rice taste is increasingly gaining importance. Although the existing breeding technology has the technology of breeding excellent rice varieties, the existing breeding technology mainly focuses on the optimization of yield traits and disease resistance traits, and the obtained rice varieties have poor palatability; the prior high-quality slender white rice processing method is often low in the rice finishing rate; the prior art has no method for breeding conventional varieties of long grain aromatic high-quality rice.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) The prior art mainly focuses on the optimization of yield traits and disease resistance traits, and the obtained rice variety has poor palatability.
(2) The prior fine and slender rice processing method is often low in rice finishing rate.
(3) The prior art has no method for breeding conventional varieties of long grain aromatic high-quality rice.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a breeding method of a conventional variety of long grain fragrance type high-quality rice.
The invention is realized in such a way that the breeding method of the conventional variety of the long grain odor type high-quality rice comprises the following steps:
selecting parents, namely selecting a high-quality long-grain conventional rice variety as a primary female parent, and selecting an odor type conventional rice variety containing a Pigm rice blast resistance gene as a primary male parent to perform hybridization to obtain F1 generation seeds;
planting the F1 generation seeds to obtain F2 generation rice plants; performing DNA extraction based on selection of rice plants with excellent field character performance and long grains;
thirdly, performing close interlocking molecular marker detection on the extracted DNA by using a molecular marker assisted selection technology, screening rice plants which simultaneously have the fragrance genes and the Pigm rice blast resistance genes and have long grain characters as second-generation female parents, and performing backcross with the primary male parents to obtain BC1F 2-generation rice;
and step four, performing multiple selfing on the rice of BC1F2 generation, and breeding into a high-quality rice conventional variety with long-grain fragrance type and rice blast resistance according to the auxiliary selection of the fragrance gene and the Pigm rice blast resistance gene molecular marker until the BC1F8 generation.
Further, the step two of selecting rice plants with excellent field character performance and long grains based on the F2 and extracting DNA comprises the following steps:
cutting leaves of rice plants, cleaning the cut leaves, drying the cleaned leaves at 55-65 ℃, crushing the leaves by using a crusher, and sieving the crushed leaves to obtain leaf powder;
ultrasonically and uniformly mixing the leaf powder and the DNA extracting solution, and adding steel balls into the mixed liquid; heating in water bath, and performing ultrasonic oscillation;
taking out the steel balls, naturally cooling the mixed liquid to room temperature, and centrifuging; taking the supernatant, uniformly mixing the supernatant with isopropanol, and standing for a period of time at the temperature of minus 10 ℃;
and centrifuging the liquid after standing again, taking supernate, washing the supernate by using absolute ethyl alcohol, naturally drying the supernate, and adding deionized water to dissolve the supernate to obtain a DNA extracting solution.
Further, the screening comprises: sieving with 80-100 mesh sieve.
Further, the heating temperature of the water bath is 50-55 ℃.
Further, the ultrasonic oscillation time is 15-20min.
Further, the centrifuging comprises: centrifugation was carried out at 8000r/s for 15min.
Further, the detection of the aroma gene and the Pigm rice blast resistance gene tightly interlocked molecular markers based on the extracted DNA by using a molecular marker assisted selection technology in the third step comprises:
designing a specific primer based on a molecular marker RSP04 closely interlocked with rice aroma genes and a molecular marker InDel587 closely interlocked with rice blast resistance genes;
mixing the designed specific primers, the extracted DNA and water to prepare a reaction system, and performing PCR amplification to obtain a molecular marker RSP04 tightly interlocked with the fragrance gene and a molecular marker InDel587 tightly interlocked with the rice blast resistance gene;
the molecular markers RSP04 and InDel587 are adopted to carry out auxiliary selection on BC1F2 generation rice, and long-grain rice excellent single plants with fragrance genes and rice blast resistance genes are screened.
Further, multiple selfing is carried out on the rice of BC1F2 generation, and the high-quality rice conventional variety with long-grain odor type rice blast resistance is bred to BC1F8 generation by combining the auxiliary selection of molecular markers of the fragrance gene and the Pigm rice blast resistance gene.
By combining all the technical schemes, the invention has the advantages and positive effects that: the invention breeds a high-quality conventional rice variety with long and thin grains, rice blast resistance, fragrance and good palatability; the invention combines the conventional pedigree method with the molecular marker assisted breeding technology to breed the conventional variety of the long grain odor type high-quality rice combining the advantages of parents, improves the production benefit of planting farmers and meets the higher requirement of people on the taste quality of the rice.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
FIG. 1 is a flow chart of a breeding method of a conventional variety of long grain aromatic high-quality rice provided by an embodiment of the invention.
FIG. 2 is a flow chart of a method for detecting a scent gene and a Pigm rice blast resistance gene based on extracted DNA by using a molecular marker assisted selection technology provided by an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a breeding method of a conventional variety of long grain fragrance type high-quality rice, and the invention is described in detail with reference to the accompanying drawings.
As shown in fig. 1, the breeding method of the conventional variety of the long grain aroma type high-quality rice provided by the embodiment of the invention comprises the following steps:
s101, performing parent selection, selecting a high-quality long-grain conventional rice variety as a primary female parent, and selecting an odor type conventional rice variety containing a Pigm rice blast resistance gene as a primary male parent to perform hybridization to obtain F1 generation seeds;
s102, planting the F1 generation seeds to obtain F2 generation rice plants; performing DNA extraction based on selection of rice plants with excellent field character performance and long grains;
s103, performing close interlocking molecular marker detection on the extracted DNA by using a molecular marker assisted selection technology, screening rice plants which simultaneously have the fragrance genes and the Pigm rice blast resistance genes and have long grain characters as second-generation female parents, and performing backcross with the primary male parents to obtain BC1F 2-generation rice;
and S104, selfing for multiple times based on the rice of BC1F2 generation, and breeding a high-quality rice conventional variety with long-grain fragrance type and rice blast resistance to BC1F8 generation by combining with the auxiliary selection of the fragrance gene and the Pigm rice blast resistance gene molecular marker.
The method for extracting the DNA of the rice plant which is excellent in field character performance and long in grain based on selection provided by the embodiment of the invention comprises the following steps:
cutting leaves of rice plants, cleaning the cut leaves, drying the cleaned leaves at 55-65 ℃, crushing the leaves by using a crusher, and sieving the crushed leaves to obtain leaf powder;
ultrasonically and uniformly mixing the leaf powder and the DNA extracting solution, and adding steel balls into the mixed liquid; heating in water bath, and performing ultrasonic oscillation;
taking out the steel ball, naturally cooling the mixed liquid to room temperature, and centrifuging; taking the supernatant, uniformly mixing the supernatant with isopropanol, and standing for a period of time at the temperature of minus 10 ℃;
and centrifuging the liquid after standing again, taking supernate, washing the supernate by using absolute ethyl alcohol, naturally drying the supernate, and adding deionized water to dissolve the supernate to obtain a DNA extracting solution.
Further, the screening comprises: sieving with 80-100 mesh sieve.
Further, the heating temperature of the water bath is 50-55 ℃.
Further, the ultrasonic oscillation time is 15-20min.
Further, the centrifuging comprises: centrifugation was carried out at 8000r/s for 15min.
As shown in FIG. 2, the detection of closely interlocked molecular markers of scent genes and Pigm rice blast resistance genes based on extracted DNA using molecular marker assisted selection technology provided by the embodiments of the present invention comprises:
s201, designing a specific primer based on a molecular marker RSP04 tightly interlocked with rice aroma genes and a molecular marker InDel587 tightly interlocked with rice blast resistance genes;
s202, mixing and preparing a reaction system based on the designed specific primers and the extracted DNA and water, and carrying out PCR amplification to obtain a molecular marker RSP04 tightly interlocked with the fragrance gene and a molecular marker InDel587 tightly interlocked with the rice blast resistance gene;
the molecular markers RSP04 and InDel587 are adopted to carry out auxiliary selection on BC1F2 generation rice, and long-grain rice excellent single plants with fragrance genes and rice blast resistance genes are screened.
Further, multiple selfing is carried out on rice of BC1F2 generation, and the high-quality rice conventional variety with the fragrance type and the rice blast resistance is bred to BC1F8 generation by combining with the auxiliary selection of the molecular markers of the fragrance gene and the Pigm rice blast resistance gene.
The embodiment of the invention carries out multiple selfing on BC1F2 generation rice, combines the auxiliary selection of molecular markers of the fragrance gene and the Pigm rice blast resistant gene, and grows into a high-quality rice conventional variety with long-grain fragrance type rice blast resistance up to BC1F8 generation.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (8)
1. A breeding method of a conventional variety of long grain odor type high-quality rice is characterized by comprising the following steps:
selecting parents, namely selecting a high-quality long-grain conventional rice variety as a primary female parent, and selecting a fragrant conventional rice variety containing Pigm rice blast resistance genes as a primary male parent for hybridization to obtain F1 generation seeds;
planting the F1 generation seeds to obtain F2 generation rice plants; performing DNA extraction based on selection of rice plants with excellent field character performance and long grains;
thirdly, performing close interlocking molecular marker detection on the extracted DNA by using a molecular marker assisted selection technology, screening rice plants which simultaneously have the fragrance gene and the Pigm rice blast resistance gene and have long grain characters as a second-generation female parent, and performing backcross with a primary male parent to obtain BC1F 2-generation rice;
and step four, performing multiple selfing on the rice of BC1F2 generation, and breeding into a high-quality rice conventional variety with long-grain fragrance type and rice blast resistance according to the auxiliary selection of the fragrance gene and the Pigm rice blast resistance gene molecular marker until the BC1F8 generation.
2. The method for breeding the conventional variety of long grain aromatic high-quality rice as claimed in claim 1, wherein the step two of selecting long-grain rice plants with excellent field performance based on F2 and performing DNA extraction comprises:
cutting leaves of rice plants, cleaning the cut leaves, drying the leaves at the temperature of 55-65 ℃, crushing the leaves by using a crusher, and sieving the crushed leaves to obtain leaf powder;
ultrasonically and uniformly mixing the leaf powder and the DNA extracting solution, and adding steel balls into the mixed liquid; heating in water bath, and performing ultrasonic oscillation;
taking out the steel balls, naturally cooling the mixed liquid to room temperature, and centrifuging; taking the supernatant, uniformly mixing the supernatant with isopropanol, and standing for a period of time at the temperature of minus 10 ℃;
and centrifuging the liquid after standing again, taking supernate, washing the supernate by using absolute ethyl alcohol, naturally drying the supernate, and adding deionized water to dissolve the supernate to obtain a DNA extracting solution.
3. The method for breeding the conventional variety of the long grain aromatic high-quality rice as claimed in claim 2, wherein the screening comprises: sieving with 80-100 mesh sieve.
4. The method for breeding the conventional variety of the long grain aromatic high-quality rice as claimed in claim 2, wherein the water bath heating temperature is 50-55 ℃.
5. The method for breeding the conventional variety of long grain aromatic high-quality rice as claimed in claim 2, wherein the ultrasonic oscillation time is 15-20min.
6. The method for breeding the conventional variety of the long grain aromatic high-quality rice as claimed in claim 2, wherein the centrifugation comprises: centrifugation was carried out at 8000r/s for 15min.
7. A method for breeding a conventional variety of long grain odor type high quality rice as claimed in claim 1, wherein the step three of utilizing molecular marker assisted selection technology to perform the detection of the closely interlocked molecular markers of the aroma gene and the Pigm rice blast resistant gene based on the extracted DNA comprises:
designing a specific primer based on a molecular marker RSP04 closely interlocked with rice aroma genes and a molecular marker InDel587 closely interlocked with rice blast resistance genes;
mixing the designed specific primers, the extracted DNA and water to prepare a reaction system, and performing PCR amplification to obtain a molecular marker RSP04 tightly interlocked with the fragrance gene and a molecular marker InDel587 tightly interlocked with the rice blast resistance gene;
the molecular markers RSP04 and InDel587 are adopted to carry out auxiliary selection on BC1F2 generation rice, and long-grain rice excellent single plants with fragrance genes and rice blast resistance genes are screened.
8. The breeding method of conventional varieties of long grain odor type high quality rice as claimed in claim 1, wherein the conventional varieties of long grain odor type high quality rice are bred by multiple selfing based on BC1F3 generation rice and combining with the auxiliary selection of molecular markers of odor genes and Pigm rice blast resistance genes. The invention breeds a high-quality conventional rice variety with super-slender grains, rice blast resistance, fragrance and good palatability.
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Citations (6)
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| CN101921761A (en) * | 2008-04-18 | 2010-12-22 | 山东省水稻研究所 | Molecular marker for co-separating from aroma character |
| CN109618916A (en) * | 2019-02-02 | 2019-04-16 | 福建农林大学 | A kind of selection of long grain odor type High quality glutinous rice conventional variety |
| CN110169354A (en) * | 2019-06-19 | 2019-08-27 | 湖南杂交水稻研究中心 | A kind of pyramiding breeding method of blast resisting, excellent flavour rice kind |
| CN111011205A (en) * | 2019-12-27 | 2020-04-17 | 江苏里下河地区农业科学研究所 | Method for creating low-chalkiness high-rice-blast-resistance fragrant soft rice germplasm |
| CN111670805A (en) * | 2020-07-13 | 2020-09-18 | 江苏沿海地区农业科学研究所 | Breeding method of new long-grain fragrant soft disease-resistant japonica rice variety |
| CN111903500A (en) * | 2020-07-31 | 2020-11-10 | 广西壮族自治区农业科学院 | Breeding method of fragrant rice blast resistant restorer cinnamon 5886 with high harvest index |
-
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- 2022-11-08 CN CN202211393054.XA patent/CN115735763A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921761A (en) * | 2008-04-18 | 2010-12-22 | 山东省水稻研究所 | Molecular marker for co-separating from aroma character |
| CN109618916A (en) * | 2019-02-02 | 2019-04-16 | 福建农林大学 | A kind of selection of long grain odor type High quality glutinous rice conventional variety |
| CN110169354A (en) * | 2019-06-19 | 2019-08-27 | 湖南杂交水稻研究中心 | A kind of pyramiding breeding method of blast resisting, excellent flavour rice kind |
| CN111011205A (en) * | 2019-12-27 | 2020-04-17 | 江苏里下河地区农业科学研究所 | Method for creating low-chalkiness high-rice-blast-resistance fragrant soft rice germplasm |
| CN111670805A (en) * | 2020-07-13 | 2020-09-18 | 江苏沿海地区农业科学研究所 | Breeding method of new long-grain fragrant soft disease-resistant japonica rice variety |
| CN111903500A (en) * | 2020-07-31 | 2020-11-10 | 广西壮族自治区农业科学院 | Breeding method of fragrant rice blast resistant restorer cinnamon 5886 with high harvest index |
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