CN109618916A - A kind of selection of long grain odor type High quality glutinous rice conventional variety - Google Patents
A kind of selection of long grain odor type High quality glutinous rice conventional variety Download PDFInfo
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- CN109618916A CN109618916A CN201910106804.2A CN201910106804A CN109618916A CN 109618916 A CN109618916 A CN 109618916A CN 201910106804 A CN201910106804 A CN 201910106804A CN 109618916 A CN109618916 A CN 109618916A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of selections of long grain odor type High quality glutinous rice conventional variety, belong to Special Rices breeding technical field, it is with the rice varieties A containing Pigm blast resistant gene, the long dulcet low amylose content rice varieties B of grain, High quality glutinous rice kind C is parent, pass through hybridization, it reestablishes diplomatic relations and pedigree method selects, the technologies such as Characters Identification, realize merit polymerization, it is good finally to select leaf morphology, moderate growth duration, and the grain of rice is elongated, odor type, disease-resistant glutinous rice strain, by its through plus be commissioned to train support after, obtain the high-quality long disease-resistant glutinous rice conventional variety of grain odor type for stablizing heredity.The long grain odor type High quality glutinous rice conventional variety of gained can meet in the market the needs of to extraordinary glutinous rice product, there is popularization and application potential well.
Description
Technical field
The invention belongs to Special Rices breeding technical fields, and in particular to a kind of choosing of long grain odor type High quality glutinous rice conventional variety
Educate method.
Background technique
With the raising of living standards of the people and the adjustment of Crop structure, sufficiently excavates and provided using precious extraordinary rice
Source, reinforces high-quality seed rice resource innovation, expand name, spy, excellent rice cultivated area, meet the growing need in domestic and international market
It wants, has been a main trend of current eating rice production.Glutinous rice has long plantation to go through as the small kind in China's rice-cultivating
History and germ plasm resource abundant.It is glutinous to contain protein, fat, carbohydrate, calcium, phosphorus, iron, the nutritional ingredients such as vitamin B1, B2, with benefit
Middle QI invigorating, warming spleen and stomach and other effects.Many traditional characteristics food, such as the rice dumpling, rice wine, glutinous rice cake can be processed by raw material of glutinous rice
Is liked deeply by the majority of consumers Deng, these food.Since the germ plasm resource of glutinous rice is few, can directly plant development excellent kind
It is few.Glutinous rice conventional variety yield is generally lower than non-glutinous rice, and that there are resistances is poor, adaptability is narrow, palatability is poor, is widely applied difficulty
Greatly, the problems such as economic benefit is low, a large amount of peasants abandon plantation glutinous rice.Therefore, initiative has distinctive long grain, high-quality, disease-resistant, fragrant
Type, and the glutinous rice rice varieties that palatability is good, are the breeding objectives of current glutinous rice.
Summary of the invention
The purpose of the present invention is to provide a kind of selections of long grain odor type High quality glutinous rice conventional variety, and what is be bred as is glutinous
Rice conventional variety leaf morphology is good, moderate growth duration, and has the spies such as distinctive long grain, odor type, high-quality, disease-resistant and palatability be good
Point can meet in the market the needs of to extraordinary glutinous rice product.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of selection of long grain odor type High quality glutinous rice conventional variety, is to choose the rice containing Pigm blast resistant gene
Kind A, the long dulcet low amylose content rice varieties B of grain, it is close that rice matter, which is accredited as the good glutinous rice variety C of national standard,
This, by hybridizing, reestablishing diplomatic relations and the technologies such as pedigree method selection, Characters Identification, selection leaf morphology is good, moderate growth duration, and the grain of rice
Elongated, odor type, disease-resistant glutinous rice strain, by its through plus be commissioned to train support after, obtain stablize heredity the high-quality long disease-resistant glutinous rice of grain odor type
Conventional variety.
The rice varieties A containing Pigm blast resistant gene is " Gu Mei 4 "；The long dulcet low amylose of grain
Content rice varieties B be " Yu Zhenxiang ", 9.0 millimeters of Rice Kernel grain length, length-width ratio 4.8, Coarse Rice Rate 80.0%, chalky grain rate
3%, chalkiness degree 0.4%, 1 grade of transparency；It is " great Feng is glutinous ", gel consistence that rice matter used, which is accredited as the good glutinous rice variety C of national standard,
100 millimeters, amylose content 2.0%~2.1%, length-width ratio 2.7~2.9,88~90 points of Cooking Quality (according to GB/T
15682-2008 " grain and oil detection paddy, rice cooking edible quality sensory evaluation methods " carry out food flavor identification).
The selection of the long grain odor type High quality glutinous rice conventional variety the following steps are included:
(1) the rice varieties A for containing Pigm blast resistant gene is chosen as male parent, and rice matter is accredited as the good glutinous rice product of national standard
Kind C obtains F as female parent, by hybridization1(C/A)；Make male parent, rice with the dulcet low amylose content rice varieties B of long grain
Matter is accredited as the good glutinous rice variety C of national standard as female parent, obtains F by hybridization1(C/B)；Cross combination F is planted respectively1(C/A)、
F1(C/B)And reestablish diplomatic relations, obtain F1(C/B//C/A), plantation, which is reestablished diplomatic relations, combines F1(C/B//C/A), obtain F2For seed；
(2) by F2It is sowed for seed, extracts rice leaf DNA, be pair with the rice varieties A containing Pigm blast resistant gene
According to using the molecular labeling InDel587 with resistant gene close linkage to F2Assisted Selection is marked for each single plant, so
It selects the plant containing resistant gene Pigm to plant afterwards and carries out Resistance screening in rice blast lesion crop field；After rice plant grows up to,
Select that seed elongated (grain >=10.0 millimeters in length, length-width ratio >=5.0), leaf morphology be good, moderate growth duration, Comprehensive Traits are excellent
Single plant strain, obtain F3Generation；
(3) use small-sized hulling mill by F3Single plant carries out out rough, and selecting Coarse Rice Rate >=80%, brown rice color is opaque white color
Strain, by gained F3Brown rice seeds are divided into two；It is control with High quality glutinous rice kind C, by itself and half F3Brown rice seeds are removed respectively
It is rough, be ground up, sieved after carry out amylose content determination, select the rice strain of amylose≤2.1%；It is savory with long grain
Low amylose content rice varieties B be control, by the other half brown rice of the rice strain for amylose≤2.1% chosen
It takes 30 to be placed in the vernalization box for filling distilled water, after being placed in 38 DEG C of incubator presoaking and germinating 12h, opens one by one, use nose immediately
It is smelt, identifies fragrance, eliminates the strain of British plain spirits；
(4) by long grain selected by step (3), with fragrance and there is waxy F3Strain plantation is resisted in rice blast lesion crop field
Disease screening；The water intaking fresh blade of rice identifies fragrance with KOH infusion method, eliminates British plain spirits strain；Strain to be chosen is eared mature
When, therefrom select that plant type is moderate, yielding ability is good, seed elongated (grain length >=10mm, length-width ratio >=5), Comprehensive Traits are excellent
Rice single plant obtains F4Seed；Step (3) are repeated to gained F4Coarse Rice Rate, amylose content and the fragrance of seed reflect
It is fixed, and food flavor identification is carried out to selected strain rice, Cooking Quality >=85 point are selected, mouthfeel is excellent, palatability is good and tool fragrance
Strain；
(5) by F selected by step (4)4Strain is sowed by strain, repeats step (2), (3), antagonism, grain shape, brown rice in (4)
Rate, amylose content, fragrance and food flavor are identified that harvest meets the F of breeding objective5For seed strain；Final warp plus generation
To 7 generations, Other Main Agronomic Characters are stablized, and acquisition yielding ability is good, Heading date and plant height is moderate, rice is in slender type, length and width ratio
Greatly, the good high-quality odor type glutinous rice conventional variety of palatability.
The present invention has the advantage that compared with the prior art
The present invention provides a kind of selection of long grain odor type High quality glutinous rice conventional variety, this method is related to long grain, odor type, excellent
The good glutinous rice rice variety selective of matter, disease-resistant and palatability can meet in the market the needs of to extraordinary glutinous rice product.It specifically has
Have the advantage that (1) improves the resistance of High quality glutinous rice.It is parent with high yield and high quality conventional variety and disease-resistant variety, using routine
Pedigree method is combined with molecular mark technology, and it is good containing resistant gene, yielding ability to cultivate, the wide glutinous rice of adaptability
New varieties；(2) alienation of glutinous rice poor appearance is improved, the commodity value in market is improved.It cultivates with speciality grain, leptosomatic difference
The glutinous rice kind of property appearance, realizes the differentiation with the common glutinous rice commodity in market；(3) under the premise of cultivating fragrance glutinous rice,
High-quality conventional variety characteristic is made full use of, food flavor identification is carried out, selects the High quality glutinous rice that mouthfeel is excellent, palatability is good and has fragrance
Kind.
Detailed description of the invention
Fig. 1 is used as the High quality glutinous rice kind " great Feng is glutinous " of parent and the High quality glutinous rice routine product selected by embodiment 1
The seed length vs figure of kind " fragrant glutinous No. 1 long ".
Fig. 2 is used as the High quality glutinous rice kind " great Feng is glutinous " of parent, rice varieties " Yu Zhenxiang " by embodiment 1 and selected
High quality glutinous rice conventional variety " long fragrant glutinous No. 1 " sample controls figure.
Specific embodiment
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
Embodiment:
(1) it chooses the rice varieties A " Gu Mei 4 " containing Pigm blast resistant gene and is used as male parent, it is excellent that rice matter is accredited as national standard
The glutinous rice variety C " great Feng is glutinous " of matter (100 millimeters of gel consistence, eat by amylose content 2.0%~2.1%, length-width ratio 2.7~2.9
88~90 points of flavor quality) as female parent, F is obtained by hybridization1(C/A)；With the dulcet low amylose content rice product of long grain
Kind B " Yu Zhenxiang " (length-width ratio 4.8, Coarse Rice Rate 80.0%) makees male parent, and High quality glutinous rice kind C " great Feng is glutinous " is as female parent, by miscellaneous
Friendship obtains F1(C/B)；Cross combination F is planted respectively1(C/A)、F1(C/B)And reestablish diplomatic relations, obtain F1(C/B//C/A), plant combination of reestablishing diplomatic relations
F1(C/B//C/A), obtain F2For seed；
(2) by F2It is sowed for seed, extracts rice leaf DNA, be pair with the rice varieties A containing Pigm blast resistant gene
According to using the molecular labeling InDel587 with resistant gene close linkage to F2Assisted Selection is marked for each single plant, so
It selects the plant containing resistant gene Pigm to plant afterwards and carries out Resistance screening in rice blast lesion crop field；After rice plant grows up to,
Select that seed elongated (grain >=10.0 millimeters in length, length-width ratio >=5.0), leaf morphology be good, moderate growth duration, Comprehensive Traits are excellent
Single plant strain, obtain F3Generation；
(3) use small-sized hulling mill by F3Single plant carries out out rough, and selecting Coarse Rice Rate >=80%, brown rice color is opaque white color
Strain, by gained F3Brown rice seeds are divided into two；It is control with High quality glutinous rice kind C " great Feng is glutinous ", by itself and half F3Brown rice kind
Son respectively except it is rough, be ground up, sieved after carry out amylose content determination, select the rice strain of amylose≤2.1%；With water
Rice varieties B " Yu Zhenxiang " is control, takes 30 to be placed in the other half brown rice of the rice strain for amylose≤2.1% chosen
Fill in the vernalization box of 20ml distilled water, after being placed in 38 DEG C of incubator presoaking and germinating 12h, open one by one, immediately with nasil it, mirror
Fixation taste eliminates the strain of British plain spirits；
(4) by long grain selected by step (3), with fragrance and there is waxy F3Strain plantation is resisted in rice blast lesion crop field
Disease screening；The water intaking fresh blade of rice identifies fragrance with KOH infusion method, eliminates British plain spirits strain；Strain to be chosen is eared mature
When, therefrom select that plant type is moderate, yielding ability is good, seed elongated (grain length >=10mm, length-width ratio >=5), Comprehensive Traits are excellent
Rice single plant obtains F4Seed；Step (3) are repeated to gained F4Coarse Rice Rate, amylose content and the fragrance of seed reflect
It is fixed, and according to GB/T 15682-2008 " grain and oil detection paddy, rice cooking edible quality sensory evaluation method " to selected
Strain rice carries out food flavor identification, selects Cooking Quality >=85 point, and mouthfeel is excellent, palatability is good and has the strain of fragrance；
(5) by F selected by step (4)4Strain is sowed by strain, repeats step (2), (3), antagonism, grain shape, brown rice in (4)
Rate, amylose content, fragrance and food flavor are identified that harvest meets the F of breeding objective5For seed strain；Final warp plus generation
To 7 generations, Other Main Agronomic Characters are stablized, and acquisition yielding ability is good, Heading date and plant height is moderate, rice is in slender type, length and width ratio
Greatly, the good high-quality odor type glutinous rice conventional variety of palatability " fragrant glutinous No. 1 long ".
As shown in Figure 1, the paddy seed grain length 9.1mm of parent " great Feng is glutinous ", wide 2.9 mm of grain, length-width ratio 3.1；Breeding
" long fragrant glutinous No. 1 " paddy seed grain length 11.5mm, wide 1.9 mm of grain, length-width ratio 6.1.Compare control " great Feng is glutinous ", breeding
" long fragrant glutinous No. 1 " grain be slender type (by GT/T 19557.7-2004 " new variety of plant specificity, consistency and stability
Testing Guideline rice " in brown rice length grading standard, 7 millimeters of brown rice length > are long grain).
As shown in Fig. 2, " fragrant glutinous No. 1 long " brown rice length-width ratio of breeding is bigger, and the grain of rice is more thin compared with parent " Yu Zhenxiang "
It is long.
The amylose content of " long fragrant glutinous No. 1 " of breeding is 2.0%, paddy seed grain length 11.5mm, wide 1.9 mm of grain,
Length-width ratio is 6.1；It is thin to reach the grain of rice for the sharp sharp spindle of brown rice, 8.6 mm of brown rice grain length, wide 1.5 mm of grain, length-width ratio 5.7
It is long；Kind Cooking Quality 87 divides, and fragrance is medium, and 2 grades of blast resisting, the early season time of infertility 105-108 days or so, plant height
113.4cm, per acre effective 24.3 ten thousand fringe of fringe, every total grain panicle number 132, setting percentage 78.64%, mass of 1000 kernel 16.74g, amylose
Content 2.0%, gel consistence 102.0mm.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (3)
1. a kind of selection of long grain odor type High quality glutinous rice conventional variety, it is characterised in that: choose and contain Pigm blast resisting
The rice varieties A, the long dulcet low amylose content rice varieties B of grain, High quality glutinous rice kind C of gene are parent, are passed through
Hybridize, reestablish diplomatic relations and the technology of pedigree method selection, Characters Identification, choose that leaf morphology is good, moderate growth duration, and the grain of rice is elongated, fragrant
Type, disease-resistant glutinous rice strain, by its through plus be commissioned to train support after, obtain stablize heredity the high-quality long disease-resistant glutinous rice routine product of grain odor type
Kind.
2. the selection of long grain odor type High quality glutinous rice conventional variety according to claim 1, it is characterised in that: including with
Lower step:
(1) the rice varieties A for containing Pigm blast resistant gene is chosen as male parent, and High quality glutinous rice kind C passes through as female parent
Hybridization obtains F1(C/A)；Make male parent with the dulcet low amylose content rice varieties B of long grain, High quality glutinous rice kind C is as female
This, obtains F by hybridization1(C/B)；Cross combination F is planted respectively1(C/A)、F1(C/B)And reestablish diplomatic relations, obtain F1(C/B//C/A), plantation
It reestablishes diplomatic relations and combines F1(C/B//C/A), obtain F2For seed；
(2) by F2It is sowed for seed, extracts rice leaf DNA, be control with the rice varieties A containing Pigm blast resistant gene,
Using the molecular labeling InDel587 with resistant gene close linkage to F2Assisted Selection is marked for each single plant, then selects
It selects the plant plantation containing resistant gene Pigm and carries out Resistance screening in rice blast lesion crop field；After rice plant grows up to, selection
Grain is >=10.0 millimeters long, length-width ratio >=5.0, leaf morphology are good, moderate growth duration, and the excellent single plant strain of Comprehensive Traits obtains
Obtain F3Generation；
(3) by F3Single plant carries out out rough, selects Coarse Rice Rate >=80%, brown rice color for the strain of opaque white color, by gained F3It is rough
Rice seed is divided into two；It is control with High quality glutinous rice kind C, by itself and half F3Brown rice seeds remove respectively it is rough, be ground up, sieved after
Amylose content determination is carried out, the rice strain of amylose≤2.1% is selected；By the water for amylose≤2.1% chosen
The other half brown rice of rice strain takes 30 to be placed in the vernalization box for filling distilled water, after 38 DEG C of presoaking and germinating 12h, opens one by one
It identifies fragrance, eliminates the strain of British plain spirits；
(4) by long grain selected by step (3), with fragrance and there is waxy F3Strain plantation is resisted in rice blast lesion crop field
Disease screening；The water intaking fresh blade of rice identifies fragrance with KOH infusion method, eliminates British plain spirits strain；Strain to be chosen is eared mature
When, therefrom select that plant type is moderate, yielding ability is good, grain is long >=10mm, length-width ratio >=5, the excellent rice single plant of Comprehensive Traits obtains
Obtain F4Seed；Step (3) are repeated to gained F4Coarse Rice Rate, amylose content and the fragrance of seed identified, and to selected
Strain rice carries out food flavor identification, and selection mouthfeel is excellent, palatability is good and has the strain of fragrance；
(5) by F selected by step (4)4Strain is sowed by strain, repeats step (2), (3), antagonism, grain shape, brown rice in (4)
Rate, amylose content, fragrance and food flavor are identified that harvest meets the F of breeding objective5For seed strain；Final warp plus generation
To 7 generations, Other Main Agronomic Characters are stablized, and acquisition yielding ability is good, Heading date and plant height is moderate, rice is in slender type, length and width ratio
Greatly, the good high-quality odor type glutinous rice conventional variety of palatability.
3. the selection of long grain odor type High quality glutinous rice conventional variety according to claim 1 or 2, it is characterised in that: institute
Stating the rice varieties A containing Pigm blast resistant gene is " Gu Mei 4 "；The long dulcet low amylose content rice product of grain
Kind of B is " Yu Zhenxiang ", 9.0 millimeters of Rice Kernel grain length, length-width ratio 4.8；High quality glutinous rice kind C used is " great Feng is glutinous ",
100 millimeters of gel consistence, amylose content 2.0%~2.1%, length-width ratio 2.7~2.9.
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Cited By (6)
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| CN111011205A (en) * | 2019-12-27 | 2020-04-17 | 江苏里下河地区农业科学研究所 | Method for creating low-chalkiness high-rice-blast-resistance fragrant soft rice germplasm |
| CN113179940A (en) * | 2020-01-14 | 2021-07-30 | 亿科国际种业有限公司 | Breeding method of purple-leaf black-japonica fragrant rice variety |
| CN113179941A (en) * | 2020-01-14 | 2021-07-30 | 亿科国际种业有限公司 | Breeding method of' Ganyouxiang No. 3 |
| CN113875579A (en) * | 2021-10-22 | 2022-01-04 | 宁波市农业科学研究院 | Breeding method of rice blast-resistant soft fragrant japonica rice |
| CN115735763A (en) * | 2022-11-08 | 2023-03-07 | 宜春市科学院(江西富硒产业研究院) | Breeding method of conventional variety of long-grain fragrant high-quality rice |
| CN116210581A (en) * | 2023-04-12 | 2023-06-06 | 贵州省农作物技术推广总站 | Breeding method of extra-soft red rice variety |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111011205A (en) * | 2019-12-27 | 2020-04-17 | 江苏里下河地区农业科学研究所 | Method for creating low-chalkiness high-rice-blast-resistance fragrant soft rice germplasm |
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| CN113179940A (en) * | 2020-01-14 | 2021-07-30 | 亿科国际种业有限公司 | Breeding method of purple-leaf black-japonica fragrant rice variety |
| CN113179941A (en) * | 2020-01-14 | 2021-07-30 | 亿科国际种业有限公司 | Breeding method of' Ganyouxiang No. 3 |
| CN113875579A (en) * | 2021-10-22 | 2022-01-04 | 宁波市农业科学研究院 | Breeding method of rice blast-resistant soft fragrant japonica rice |
| CN115735763A (en) * | 2022-11-08 | 2023-03-07 | 宜春市科学院(江西富硒产业研究院) | Breeding method of conventional variety of long-grain fragrant high-quality rice |
| CN116210581A (en) * | 2023-04-12 | 2023-06-06 | 贵州省农作物技术推广总站 | Breeding method of extra-soft red rice variety |
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Application publication date: 20190416 |