CN105028175B - A kind of selection of waxy corn and white Waxy Corn Inbred Lines - Google Patents
A kind of selection of waxy corn and white Waxy Corn Inbred Lines Download PDFInfo
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- CN105028175B CN105028175B CN201510296688.7A CN201510296688A CN105028175B CN 105028175 B CN105028175 B CN 105028175B CN 201510296688 A CN201510296688 A CN 201510296688A CN 105028175 B CN105028175 B CN 105028175B
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Abstract
The invention discloses a kind of waxy corn and the selection of white Waxy Corn Inbred Lines.Wherein, the selection of the Waxy Corn Inbred Lines comprises the following steps：It is female parent by one of glutinous corn variety in some glutinous corn varieties, other glutinous corn varieties are that male parent is hybridized, and form multiple cross combinations.One of cross combination is selected, pollen and the mixed in equal amounts for collecting other cross combinations are uniform.The cross combination chosen is pollinated using well mixed pollen, obtains pollen and the target hybrid seed for the cross combination chosen.According to default objective trait, selfed breeding is carried out to the target hybrid seed, obtains stable Waxy Corn Inbred Lines.In above-mentioned breeding process, the seed selection that white Waxy Corn Inbred Lines then can be achieved in white-kernel step is selected and remain in increase.Pollinated by hybridization and pollen mixture, assemble favorable genes resource, the self-mating system of incubation is adapted to a variety of ecotypes, high temperature resistant and resistance to low temperature protrude, and disease-resistant resistance is stronger.
Description
Technical field
The present invention relates to plant breeding technique field, more particularly to the seed selection side of a kind of waxy corn and white Waxy Corn Inbred Lines
Method.
Background technology
With the raising of living standards of the people and the reinforcement of health care consciousness, species of the people to Waxy Maize product, battalion
Form part, appearance luster and healthcare function etc. and propose new requirement.Fresh, tasty waxy corn product is due to its flavor
It is the features such as unique, nutritious, health leisure, deep to be liked by consumers in general, plus suitable store and processing can year-round supply,
The market demand constantly expands.
Further, since Waxy Maize breeding time is shorter, cultivates relative ease, remarkable in economical benefits, make its cultivated area
Constantly expand.
But it is varied with weather in recent years, Glutinous Corn Varieties are added with new demand, does not require nothing more than quality
It is excellent, yield is high, with greater need for possessing the characteristics of wide adaptability, strong stress resistance.
Therefore, cultivate can be configured to high-quality, high yield, good stress resistance, wide adaptability Waxy Maize cenospecies from
Hand over the demand of system more and more stronger.But existing eurytopicity, high-quality, high yield waxy corn landrace are deficienter, selection choosing
The glutinous corn variety brought out is difficult to meet the market demand.
Therefore, prior art is to be developed.
The content of the invention
In view of in place of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of waxy corn and white waxy corn from
Hand over the selection of system, it is intended to solve the glutinous corn variety that existing glutinous corn variety selection obtains and be difficult to meet the market demand
The problem of.
In order to achieve the above object, this invention takes following technical scheme：
A kind of Waxy Corn Inbred Lines selection, wherein, comprise the following steps：
Some glutinous corn varieties are selected, are female parent by one of glutinous corn variety in some glutinous corn varieties, other
Glutinous corn variety is that male parent is hybridized, and forms multiple cross combinations；
One of cross combination in the multiple cross combination is selected, it is miscellaneous to collect in addition to the cross combination chosen other
The pollen of friendship combination is simultaneously uniform by the pollen mixed in equal amounts of each cross combination；
The cross combination chosen is pollinated using the well mixed pollen, obtains pollen and the hybridization group chosen
The target hybrid seed of conjunction；
According to default objective trait, selfed breeding is carried out to the target hybrid seed, obtains stable waxy corn certainly
Hand over system.
Described Waxy Corn Inbred Lines selection, wherein, some glutinous corn varieties include：Su Yu is glutinous 901, Chongqing is glutinous
930th, osmanthus is glutinous 519, Su Yu glutinous 639 and beautiful agriculture crystalline substance are glutinous；Su Yu glutinous 901 is selected as female parent.
Described Waxy Corn Inbred Lines selection, wherein, it is described choose be combined as Su Yu glutinous 901 and Chongqing glutinous 930.
Described Waxy Corn Inbred Lines selection, wherein, described collection other hybridization groups in addition to the cross combination chosen
The step of pollen of conjunction is specially：Respectively by respectively 5 plants of the plantation of the seed of 4 cross combination, after forming plant, each hybridization group is obtained
Close some grams of pollen of plant.
Described Waxy Corn Inbred Lines selection, wherein, some grams of pollen is specially 5g.
Described Waxy Corn Inbred Lines selection, wherein, the default objective trait includes：Head progeny row and plant are degeneration-resistant
Property, fruit ear commodity.
Described Waxy Corn Inbred Lines selection, wherein, the selfed breeding method specifically comprises the following steps：By institute
State target hybrid seed and plant some strains, bagging is selfed after forming plant；And select and remain neat kernel arrangement, fringe after fruit ear harvest
Line number is the fruit ear of 14 rows and full seed, and a point single fringe threshing obtains single fringe seed；Head progeny row is divided to plant in single fringe seed of acquisition,
According to default objective trait, in plant selfing 5-6 generations corresponding to selection, stable Waxy Corn Inbred Lines are obtained.
Described Waxy Corn Inbred Lines selection, wherein, methods described also includes：Stable waxy corn will be obtained to be selfed
Single fringe seed of system divides head progeny row to plant, and each head progeny row plants some strains, selects the selfing of fine individual plant bagging, conventional according to self-mating system
Selection was continuously selfed for 5~6 generations.
A kind of selection for applying the white Waxy Corn Inbred Lines of inbred line breeding method seed selection as described above, wherein, institute
State the selections of white Waxy Corn Inbred Lines and above-mentioned " forming multiple cross combinations " the step of and " obtain pollen with choosing respectively
Cross combination target hybrid seed " the step of after select and remain white-kernel.
Beneficial effect：The selection of a kind of waxy corn provided by the invention and white Waxy Corn Inbred Lines, selection performance are excellent
Good glutinous corn variety is hybridized, then takes equivalent pollen to be mixed respectively after cross combination is planted.Then using mixing
Pollen afterwards is pollinated, so as to which the excellent gene of separate sources resource be flocked together, there is provided more rich is glutinous
Corn gene combination supplies seed selection.Finally the method according still further to conventional herd breeding is bred as stable self-mating system, and preparation possesses eurytopicity, excellent
The excellent New Waxy Maize Hybrids of matter, high yield characteristics, effectively meet the market demand.
Brief description of the drawings
Fig. 1 is the method flow diagram of the Waxy Corn Inbred Lines selection of the specific embodiment of the invention.
Embodiment
The present invention provides the selection of a kind of waxy corn and white Waxy Corn Inbred Lines.To make the purpose of the present invention, technology
Scheme and effect are clearer, clear and definite, and the present invention is described in more detail for the embodiment that develops simultaneously referring to the drawings.It should manage
Solution, specific embodiment described herein only to explain the present invention, are not intended to limit the present invention.
A kind of as shown in figure 1, Waxy Corn Inbred Lines selection for the specific embodiment of the invention.The selection bag
Include following steps：
S1, some glutinous corn varieties of selection, are female parent by one of glutinous corn variety in some glutinous corn varieties, its
His glutinous corn variety is that male parent is hybridized, and forms multiple cross combinations.
Some glutinous corn varieties can be any suitable Good Quality Glutinous Corn kind or strain, can also be according to reality
The suitable glutinous corn variety of the purposive selection of border demand and quantity.In certain specific embodiment of the invention, it can select to make
With Su Yu is glutinous 901, Chongqing is glutinous 930, osmanthus is glutinous 519, Su Yu glutinous 639 and beautiful agriculture crystalline substance are glutinous, Su Yu glutinous 901 is selected as female parent.It is above-mentioned
Glutinous corn variety is that the parameter such as existing general types, specific character is well known to those skilled in the art, and is not gone to live in the household of one's in-laws on getting married herein
State.
Material based on the excellent glutinous corn variety of above-mentioned selection different geographical, pollinated by following hybridization and pollen mixture
Mode, favorable genes resource can be assembled, the self-mating system for causing to be bred as adapts to a variety of ecotypes, high temperature resistant and low temperature resistant
Performance protrudes, and disease-resistant resistance is stronger.
After the cross combination of the formation can specifically refer to the seed formed by two mixing breeds or seed plantation
The plant of formation or the life entity for possessing the genotype that the two mixing breeds are formed.For example, the cross combination can be
Leaf, fruit or waxy corn plant etc..
One of cross combination in S2, the multiple cross combination of selection, collect in addition to the cross combination chosen it
The pollen of his cross combination is simultaneously uniform by the pollen mixed in equal amounts of each cross combination.
The selection of specific cross combination can be selected according to specific situation, the cross combination chosen should have compared with
More excellent (or target) character, because its genotype ratio in the gene pool being subsequently formed is higher.The one of the present invention
In individual specific embodiment, the cross combination of selection is Su Yu glutinous 901 and Chongqing glutinous 930.
S3, using the well mixed pollen cross combination chosen is pollinated, it is miscellaneous with choosing to obtain pollen
Hand over the target hybrid seed of combination.
After step S1 obtains the seed of multiple cross combinations plant is formed, it is necessary to be planted.Choose multiple hybridization groups
It is one of as Pollon receiver (female parent) in conjunction.Obtain the pollen of other cross combination plant and its mixed in equal amounts is uniform
The plant of the cross combination to choosing pollinates afterwards.Specifically used chooses cross combination plant quantity and pollen quantity can
It is actually needed and is determined with foundation.For example, when having planted more cross combination plant, it is necessary to obtain pollen quantity then compared with
It is more.The method of specific pollen mixing can use existing conventional mixed method, such as stirring etc..
After the pollen of each cross combination of above-mentioned mixing again with choose cross combination to be hybridized by the way of, can will not
The excellent gene of same source (the i.e. above-mentioned glutinous corn variety used) flock together, and are provided for follow-up selfed breeding
One huge gene pool, multiple excellent genetic integration can be realized, there is the larger glutinous jade for selecting suitable trait
The possibility of rice kind.
S4, according to default objective trait, selfed breeding is carried out to the target hybrid seed, obtains stable waxy corn
Self-mating system.
After target hybrid seed (being formed by pollen mixture and the cross combination chosen) is obtained, plantation is formed after plant i.e.
Its specific character of observable.The method of selfed breeding can be used to obtain finally stable Waxy Corn Inbred Lines.Specific selfing
Selection can use existing conventional selfed breeding method, can also be according to the specific selfed breeding side needed to routine
Method is for further adjustments.
The objective trait refers to the specific character requirement of target seed selection plant, such as tassel row number, plant resistance, fruit ear
Turgor etc..In certain specific embodiment of the invention, the default objective trait specifically includes：Tassel row number, plant
The commodity of resistance, fruit ear.Wherein, the commodity of fruit ear is well known to those skilled in the art, and refers to the business for influenceing fruit ear
The specific character such as product outward appearance, such as the neat degree of fruit ear, if it is full, whether size consistent etc..
It should be noted that sequence number before above-mentioned steps is only representing the logical relation between step, rather than limit
Determine the operation order between step.Those skilled in the art the order of above-mentioned steps can be exchanged in practical operation or
Person increases/economization other suitable steps.
Specifically, the method for the selfed breeding specifically includes：
First, the target hybrid seed is planted into some strains, bagging is selfed after forming plant；And selected after fruit ear harvest
Stay neat kernel arrangement, the fruit ear that tassel row number is 14 rows and full seed, a point single fringe threshing obtains single fringe seed.
Then, head progeny row is divided to plant in single fringe seed of acquisition, according to default objective trait, plant selfing corresponding to selection
In 5-6 generations, obtain stable Waxy Corn Inbred Lines.
Wherein, it is described that the target hybrid seed is planted into some strains as 20 plants.Selected with reference to above-mentioned specific embodiment
Some glutinous corn varieties, Waxy Corn Inbred Lines wide adaptability, the strong stress resistance of the stabilization finally obtained.Economical character is excellent, plants
Strain robust growth, plant type are graceful, rushed on middle, leaf color is dark green, bract consolidation, suitable dense planting.And fruit ear commodity is good, quality
Good, the pure white color of fruit ear seed, head progeny row marshalling, full seed, fruit ear are well-balanced, nutritious, have the unique taste, and pericarp is thin, glutinous
Property is strong.
Certainly, the stable Waxy Corn Inbred Lines can as outstanding basic material, carry out further seed selection or
Prepare the glutinous corn variety needed for obtaining.For example, can be further by the Waxy Corn Inbred Lines of aforementioned stable and purplish red waxy corn
The Hybrid grain color that self-mating system prepares acquisition is red and white, is not faded after boiling, be red white clearly demarcated, well can meet market
Demand.
It is preferred that the selection can also include：Single fringe seed point of stable Waxy Corn Inbred Lines will be obtained
Head progeny row plantation, each head progeny row plant some strains, select the selfing of fine individual plant bagging, continuous certainly according to self-mating system conventional herd breeding method
Handed over for 5~6 generations.More excellent Waxy Corn Inbred Lines can further be selected by the method for above-mentioned further inbred line breeding.Its
In, it is specially 15 plants that each head progeny row, which plants some strains,.
Present invention also offers a kind of method using the above-mentioned white Waxy Corn Inbred Lines of selection seed selection.The seed selection is white
The method of waxy corn is specially respectively in step S1 " forming multiple cross combinations " and " obtaining pollen and choose for step S2
The target hybrid seed of cross combination " only selects and remain white-kernel afterwards.Selecting and remain white-kernel can ensure to be selfed in follow-up waxy corn
It is white waxy corn so as to realize the seed selection of white Waxy Corn Inbred Lines in Breeding Process.
One specific embodiment of selection of the present invention, it is described in detail below：
First, in March, 2010~June, 10 plants of Su Yu glutinous 901 are planted in Nanjing, Chongqing is glutinous 930, osmanthus is glutinous 519, Su Yu glutinous 639
Brilliant glutinous each 5 plants with beautiful agriculture.It is female parent with Su Yu glutinous 901, respectively so that glutinous 930, osmanthus of changing is glutinous 519, Su Yu glutinous 639 and beautiful agriculture is brilliant glutinous is
Male parent hybridizes 2 fringes respectively, obtains glutinous 901X Chongqings glutinous 639 Hes of the glutinous glutinous glutinous 901X Su Yu of glutinous 519, the Su Yu in 901X osmanthus of 930, Su Yu of Su Yu
The seed of brilliant glutinous 4 cross combination of the glutinous 901X jade agricultures of Su Yu, separates threshing, selects and remain white-kernel.
Secondly, in July, 2010~September, each 5 plants of 4 cross combinations are planted in Nanjing, Post flowering takes the glutinous 901X of Su Yu respectively
Brilliant 5 grams glutinous of the pollen of the glutinous 901X jade agricultures of the glutinous 901X Su Yu glutinous 639 and Su Yu of glutinous 519, the Su Yu in osmanthus, gives combination Su Yu after being well mixed
Glutinous 2~3 plants of 930 hybridization pollination in glutinous 901X Chongqings, white-kernel is selected and remain in the target hybrid seed of harvest.
Again, in October, 2010~2011 year 2 months, in the target seed plantation 20 that Sanya, Hainan obtains above-mentioned steps
Strain, whole baggings are selfed, and neat kernel arrangement, the row of tassel row number 14,2~3, the fruit ear of full seed are selected and remain after seed maturity, point
Single fringe threshing.
Finally, in March, 2011~2013 year 2 months, the side in Nanjing and Sanya, Hainan according to corn inbred line conventional herd breeding
Method, and combine the selection such as plant resistance, fruit ear commodity fine individual plant in head progeny row and be continuously selfed for 5~6 generations, in 2 months 2013
It is bred as stable white Waxy Corn Inbred Lines (being named as white Waxy Corn Inbred Lines QN-51).It is and numerous by being selfed or mixing the method handed over
Grow original seed.
The above-mentioned white Waxy Corn Inbred Lines QN-51 selected feature is specially：In the In Nanjing spring in the time of infertility
Broadcast 88 days, autumn sowing 71 days or so.Seedling leaf sheath aubergine, dark green leaf color, cane green, aerial root is flourishing, capacity for the resistance to lodging is strong.Strain
High 156 centimetres, 51 centimetres of Ear height, total number of sheets 17~18, fringe upper blade number is 5, tassel branch number 15-18, pollen
Amount is big, and the green white of filigree, fruit ear cartridge type, 16~17 centimetres of spike length, the row of tassel row number 14~16, average row grain number 32, grain color is clean
White, thousand 329 grams of fresh weights, 228 grams of dry weight.Variable rate technology：The diseases such as anti-large and small pinta, head smut, banded sclerotial blight.
A kind of selection for applying the white Waxy Corn Inbred Lines of inbred line breeding method seed selection as described above, wherein, institute
State the selections of white Waxy Corn Inbred Lines and above-mentioned " forming multiple cross combinations " the step of and " obtain pollen with choosing respectively
Cross combination target hybrid seed " the step of after select and remain white-kernel.
It is understood that for those of ordinary skills, can be with technique according to the invention scheme and this hair
Bright design is subject to equivalent substitution or change, and all these changes or replacement should all belong to the guarantor of appended claims of the invention
Protect scope.
Claims (3)
1. a kind of selection of Waxy Corn Inbred Lines, it is characterised in that comprise the following steps：
Glutinous corn variety is selected, is female parent by one of glutinous corn variety in glutinous corn variety, other glutinous corn varieties are
Male parent is hybridized, and forms multiple cross combinations；
One of cross combination in the multiple cross combination is selected, collects other hybridization groups in addition to the cross combination chosen
The pollen of conjunction is simultaneously uniform by the pollen mixed in equal amounts of each cross combination；
The cross combination chosen is pollinated using the well mixed pollen, obtains pollen and the cross combination chosen
Target hybrid seed；
According to default objective trait, selfed breeding is carried out to the target hybrid seed, obtains stable Waxy Corn Inbred Lines；
The glutinous corn variety includes：Su Yu is glutinous 901, Chongqing is glutinous 930, osmanthus is glutinous 519, Su Yu glutinous 639 and beautiful agriculture crystalline substance are glutinous；Su Yu is glutinous
901 are selected as female parent；
The collection is specially the step of the pollen of other cross combinations in addition to the cross combination chosen：Respectively by 4 hybridization groups
Respectively 5 plants of the plantation of the seed of conjunction, after forming plant, obtains the pollen of each cross combination plant；
The default objective trait includes：Head progeny row and plant resistance, fruit ear commodity；
The selfed breeding method specifically comprises the following steps：
The target hybrid seed is planted into some strains, bagging is selfed after forming plant；And seed row is selected and remain after fruit ear harvest
The fruit ear that row are neat, tassel row number is 14 rows and full seed, a point single fringe threshing obtain single fringe seed；By single fringe seed of acquisition point
Head progeny row is planted, and according to default objective trait, in plant selfing 5-6 generations corresponding to selection, obtains stable Waxy Corn Inbred Lines.
2. the selection of Waxy Corn Inbred Lines according to claim 1, it is characterised in that described to obtain each cross combination
The pollen of plant is 5g.
A kind of 3. white Waxy Corn Inbred Lines of selection seed selection for applying the Waxy Corn Inbred Lines as described in claim 1-2 is any
Selection, it is characterised in that " forming multiple cross combinations " the step of and " obtain pollen and the hybridization group chosen respectively
White-kernel is selected and remain after the step of target hybrid seed of conjunction ".
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105961186A (en) * | 2016-05-11 | 2016-09-28 | 南京市蔬菜科学研究所 | Breeding method of excellent super sweet corn inbreeding line |
| CN106962178A (en) * | 2017-04-26 | 2017-07-21 | 重庆市农业科学院 | A kind of selection of black waxy corn cenospecies |
| CN115623982A (en) * | 2022-09-09 | 2023-01-20 | 邯郸市农业科学院 | Breeding method of drought-tolerant and disease-resistant corn |
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