CN103782895A - Synthetic method of original colony for corn breeding for reciprocal recurrent selection - Google Patents
Synthetic method of original colony for corn breeding for reciprocal recurrent selection Download PDFInfo
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Abstract
The invention discloses a synthetic method of an original colony for corn breeding for reciprocal recurrent selection, belonging to the technical field of corn breeding, which solves the problem that existing gene linkage degree is high and germplasm resources are relatively narrow. The method comprises the following steps: first, selecting a plurality of selfing line materials with relatively wide hereditary basis and high common combining ability in a same class group; carrying out variance analysis and F index inspect among combining abilities of the materials based on an NCII mating designed test of an AMMI (Additive Main effects and Multiplicative Interaction) model to analyze hereditary effects; assembling incomplete diallel cross combination by using the materials; and assembling a top cross specie according to the non-overlapping principle of germplasm complementation, and after equivalently and uniformly mixing seeds of the top cross, continuously and fully mixing pollen twice to pollinate, wherein the seed obtained by the manner is the original colony for corn breeding. The synthetic method disclosed by the invention can break linkage in the same group to create variation, thereby providing an abundant material basis for recurrent selection and selfing line breeding.
Description
Technical field
The present invention relates to corn breeding techniques field, the synthetic method of initial population for especially a kind of corn breeding for Reciprocal recurrent selection.
Background technology
Corn is to utilize in the world hybrid vigour early and the crop of area maximum, in China's corn breeding long-term practice process, the narrow problem of Germplasm Bases receives much concern always, combine basic material by two inbred lines, and rely on second cycle line breeding technique and pedigree method to carry out corn inbred lines to be traditional breeding technology method, also more to have caused Germplasm Bases narrow.China is not corn cradle, germ plasm resource scarcity, and Germplasm Bases is relatively narrow.The U.S. is corn research in the world and the most flourishing country of Maize Industry, Americanologist is spent more than 80 year and is continued facts have proved of the hard stalk collective species of improvement, the good colony of artificial synthetic hereditary basis, by recurrent selection, improve gradually the frequency of beneficial gene in colony, thereby create and improve the basic material of corn breeding, very feasible.The existing Elite Maize Inbred Lines of China is the treasure of corn breeding practice of innovation, gene linkage degree is higher, with China's the most frequently used monoid inbred line synthetic population and carry out the rare report of Upgrading, particularly can thoroughly break chain synthetic method, especially rare report by design.
Summary of the invention
The object of the present invention is to provide the synthetic method of a kind of corn breeding for Reciprocal recurrent selection initial population.Overcome the not enough problem that traditional colony creates, combining ability test, degree of variation mensuration, incomplete diallel cross, topcross technology are applied to the establishment of colony, be of value to more possible chain, the increase degree of variation of breaking, improve the Breeding Efficiency of excellent corn inbred line.The present invention can break in same monoid chain, creates variation, for next step recurrent selection and inbred line breeding provide abundant material base.
The technical solution adopted in the present invention is:
A synthetic method of using initial population for the corn breeding of Reciprocal recurrent selection, is characterized in that, comprises the following steps:
S1: select that hereditary basis is relatively wide in range, general combining ability is high, belong to more than 10 inbred of same corn germplasm monoid, and material is carried out to variance analysis and F between coordinate force refer to check, hereditary effect analysis;
Described hereditary basis is relatively wide in range is: the NC II Mating design test to selected materials based on AMMI model, measure leaf, spike length, bald length on plant height, fringe position, fringe, fringe is thick, axle is thick, a row grain number, fringe are heavy, fringe grain weighs and 100-grain weight, then these genetic parameters is carried out to genetic variance and Hereditary Capacity.
The additive variance of genetic parameter is all greater than nonadditive variance, that is: additive genetic effect in the highest flight;
Broad-sense heritability is all greater than narrow-sense heritability, that is: variation has more than 50% composition to be determined by inherent cause;
Described general combining ability height is: material is carried out to F value check between coordinate force, and the F value of general combining ability all reaches significance level;
It is to belong to Characterization that described corn inbred line is chosen basic principle;
S2: first round hybridization
Carry out incomplete diallel cross with the inbred of selecting in S1 and obtain hybrid combination, each hybrid combination is gathered in the crops separately, obtains single cross hybrid seed;
S3: second takes turns hybridization
The single cross hybrid seed of gathering in the crops in step S2 is got to equivalent and plant respectively, then carry out topcross according to the not overlapping principle of combined parent strain, each topcross combination results separately, obtain topcross kind seed;
S4: third round hybridization
By the topcross kind seed of gathering in the crops in step S3, get respectively equivalent seed and mix, then get two parts of plantations of equivalent, phase mutual cross between two parts, obtains seed, is hybrid seed one;
S5: fourth round hybridization
The hybrid seed of gathering in the crops in step S4 one is mixed, then get two parts of plantations of equivalent, phase mutual cross between two parts, obtains seed, is hybrid seed two, and hybrid seed two is mixed, and obtains corn breeding initial population.
Preferably, total plant number >=4000 of two parts of plantations described in step S4.
Preferably, total plant number >=4000 of two parts of plantations described in step S5.
Beneficial effect of the present invention: synthesize maize population by the synthetic method of initial population with corn breeding of the present invention, can realize the genetic recombination of corn breeding resource break chain, for continuing improvement and preferentially carrying out inbred line breeding abundant hereditary basis is provided.
Accompanying drawing explanation
Accompanying drawing 1 is the selected inbred line material of embodiment 1 pedigree source;
Accompanying drawing 2 is the flow chart of synthetic initial population.
Embodiment
In order to understand better the present invention, further illustrate content of the present invention below in conjunction with embodiment, but content of the present invention is not only confined to the following examples, embodiment should not regard limiting the scope of the present invention as.
Below in conjunction with example, the present invention will be further described:
Embodiment 1:
A synthetic method of using initial population for the corn breeding of Reciprocal recurrent selection, comprises the following steps (as shown in Figure 2):
S1: choose following starting material (as shown in Figure 1) according to genealogical relationship: yellow early 4,502, river in Shangdong Province Huang 31413, H21, neat 310, lx9801, lx9311, prosperous 7-2, military 314, lx03-2, dredge 92-8, K12, S121, for the synthesis of colony, and verify its genetic parameter by genetic test.With frame in Fig. 1 is selected corn inbred line.It is to belong to Characterization that inbred line is chosen basic principle, from Fig. 1, mutually exists associated.
Yellow early 4: Beijing City Agriculture and Forestry Institute and the Chinese Academy of Agricultural Sciences crop in the superior corn inbred line of the common seed selection seventies, most widely used inbred line in China's tangsipingtou monoid inbred line, the outstanding advantages such as the multiple diseases such as high-combining ability, highly resistant mosaic virus, wide adaptability, plant type compactness, leaf emergence rate is fast, dual spike rate is high, calendar year 2001 is won first-class National Scientific and Technological Progress Award.It is the Inbred Lines that China is openly used.
502: Xi You seeds company of Laizhou City of Shandong Province forms in seed selection in 1991 from " 340 × yellow morning four " cross materials.Authorize corn hybrid seed west beautiful No. 3 (478 × 502), tuck in single 20(8001 × 502) apply this inbred line as parent material.It is the Inbred Lines that China is openly used.
River in Shangdong Province Huang 31413: record the seed selection of this inbred line in " Shandong agricultural science " the 4th phase in 1991.That literary composition green grass or young crops 1331 is male parent, in nineteen eighty-two assembly, forms through selfed breeding take Huang early 4 for maternal.It is parent material that corn hybrid seed cigarette list 16 (8112 × river in Shangdong Province Huang 31413), cigarette list 17 (107 × river in Shangdong Province Huang 31413) and cigarette list No. 18 (Mo17 × river in Shangdong Province Huangs 31413) are applied this selfing.It is the Inbred Lines that China is openly used.
H21: record the seed selection of this inbred line in the 21st the 3rd phase of volume of " Acta Agronomica Sinica " nineteen ninety-five.It is early 4 improvement system of corn self rotary series yellow.Beautiful 16(478 × the H21 in corn hybrid seed Shandong) to apply this selfing be parent material.It is the Inbred Lines that China is openly used.
Neat 310: recording the application of this inbred line in breeding in the 4th phase of " Shandong agricultural science " nineteen ninety, is early 4 improvement system of corn self rotary series yellow.The beautiful 11(neat 302 × neat 310 in corn hybrid seed Shandong) to apply this selfing be parent material.
Lx9801: recording the application of this inbred line in breeding in " Maize Sciences " the 3rd phase in 2003, is the improvement system of corn inbred line 502 and H21.Neat 319 × the lx9801 of the single 981(in corn hybrid seed Shandong) to apply this selfing be parent material.It is the Inbred Lines that China is openly used.
Lx9311: recording the application of this inbred line in breeding in " Maize Sciences " Z1 phase in 2007, is the inbred line that 3 corn corn inbred lines combine (Shandong former 92 × lucky 853) × lx9801 basic material seed selection.Single 6027(lx8376 × the lx9311 in corn hybrid seed Shandong) to apply this selfing be parent material.
Prosperous 7-2: be the corn inbred line of Earthquake of Anyang station in Henan institute of agricultural sciences seed selection, record the breeding and application of this inbred line in " Henan Vocation Technology Normal Inst.'s journal " the 4th phase in 2004.This inbred line is as basic material seed selection, to belong to tangsipingtou monoid take the prosperous list of single cross hybrid No. seven (yellow early four × Weihe River 95) of the institute of agricultural sciences of Changwei, Shandong (academy of agricultural sciences of existing Weifang City) seed selection.It is the Inbred Lines that China is openly used.
Military 314: record the seed selection of this inbred line and the application in breeding in " Shaanxi agricultural science " the 2nd phase in 1998.It is the Inbred Lines that China is openly used.
Lx03-2: record the application of this inbred line in breeding in " Shandong agricultural science " the 7th phase in 2008.This selfing is that (lx9801 × prosperous 7-2) seed selection forms.Single 9032(lx00-1 × the lx03-2 in authorization corn hybrid seed Shandong, Shandong Province) apply this inbred line as parent material.
Dredge 92-8: by Xunxian, the Henan Province institute of agricultural sciences (existing Hebi City academy of agricultural sciences) seed selection, record the application of this inbred line in breeding at " seed " o. 11th in 2007.Country authorization corn hybrid seed is dredged single 20(9058 × dredge 92-8) apply this inbred line as parent material.
K12: record the feature of this inbred line and the application in breeding thereof in " the modern industry of planting " the 4th phase in 2003.This inbred line is the Inbred Lines that China openly applies.
S121: record the feature of this inbred line and the application in breeding thereof in " agricultural science and technology communication " the 2nd phase in 2010, this selfing be red 340 with H201, H204 mixed pollination after through continuous 6 generations self cross and selection be bred as.Liaoning Province's authorization corn hybrid seed good beautiful No. 66 (M54 × S122) is applied this inbred line as parent material.
Material is carried out to variance analysis and the check of F value between coordinate force.
See the following form shown in 1, NC II Mating design test by 13 materials based on AMMI model, leaf, spike length, bald length on investigation plant height, fringe position, fringe, fringe is thick, axle is thick, a row grain number, fringe is heavy, fringe grain is heavy and 100-grain weight, carries out variance assessment and F value between coordinate force and tests.Fig. 2 shows, chooses inbred line GCA(general combining ability) F value all reached utmost point significance level, SCA(specific combining ability) F value all reached significance level.GCA and SCA variance ratio are all obviously greater than 1, prove investigation proterties between the combination of inbred line assembly take additive effect as main, inbred line general combining ability is high, has stronger specific combining ability.
Variance and F value between table 1 coordinate force
Material is carried out to the hereditary effect analysis of major traits.
See the following form shown in 2, NC II Mating design test by 13 materials based on AMMI model, leaf, spike length, bald length on investigation plant height, fringe position, fringe, fringe is thick, axle is thick, a row grain number, fringe are heavy, fringe grain weighs and 100-grain weight, each genetic parameter is carried out to genetic variance and Hereditary Capacity, further understand new colony Genetic Performance.Table 2 shows in selected inbred line, to have abundant genetic variation, has notable difference in colony between individuality, has larger improvement and Selection potential.The additive variance of 11 proterties is all greater than nonadditive variance, and additive genetic effect in the highest flight, improves coordinate force by colony's samsara improvement polymerization minor gene practical.Higher general combining ability is to set up the key of colony, can find out from heritability, and broad-sense heritability is all greater than narrow-sense heritability, illustrates that leaf on fringe, the variation that fringe is thick and axle is thick have more than 50% composition to be determined by inherent cause.
Genetic variance and the Hereditary Capacity of 11 proterties of table 2
S2: incomplete diallel cross
The material of preparing in step S1 is carried out to storeroom incomplete diallel cross, obtain 78 incomplete diallel cross combinations, each combination is gathered in the crops separately, obtains single cross hybrid;
Incomplete diallel cross combination is exemplified as: yellow morning 4/502, yellow early 4/ river in Shangdong Province Huang 31413, yellow early 4/H21
S3: topcross
The seed of gathering in the crops in step S2 is got to equivalent plants respectively, then carry out topcross according to the not overlapping principle of combined parent strain (4 starting materials that the female parent of topcross combination and male parent contain altogether do not overlap), obtain topcross combination and be exemplified as: yellow 31413/ neat 310, the yellow yellow 31413/lx9801 in early 4/502/ river in Shangdong Province of the yellow yellow 31413/H21 in early 4/502/ river in Shangdong Province, yellow early 4/502/ river in Shangdong Province
S4: third round hybridization
By the topcross kind seed of gathering in the crops in step S3, get respectively equivalent seed and mix, then get two parts of plantations of equivalent, plant number at least 2000 strains respectively, phase mutual cross between two parts (in every part, fully then pollen mixture pollinates between two parts mutually), obtains seed, is hybrid seed one;
S5: fourth round hybridization
The hybrid seed of gathering in the crops in step S4 one is mixed, then get two parts of plantations of equivalent, plant number at least 2000 strains respectively, phase mutual cross between two parts (in every part, fully then pollen mixture pollinates between two parts mutually), obtain seed, for hybrid seed two, hybrid seed two is mixed, obtain corn breeding initial population.
As other embodiments, colony synthetic also can select multiple other inbred lines as stock as required.
The above is the preferred embodiment of the present invention; for the practical study technical staff of the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications are also regarded as protection scope of the present invention.
Claims (3)
1. a synthetic method of using initial population for the corn breeding of Reciprocal recurrent selection, is characterized in that, comprises the following steps:
S1: select that hereditary basis is relatively wide in range, general combining ability is high, belong to more than 10 inbred of same corn germplasm monoid, and material is carried out to variance analysis and F between coordinate force refer to check, hereditary effect analysis;
Described hereditary basis is relatively wide in range is: the NC II Mating design test to selected materials based on AMMI model, measure leaf, spike length, bald length on plant height, fringe position, fringe, fringe is thick, axle is thick, a row grain number, fringe are heavy, fringe grain weighs and 100-grain weight, then these genetic parameters are carried out to genetic variance and Hereditary Capacity
The additive variance of genetic parameter is all greater than nonadditive variance, that is: additive genetic effect in the highest flight;
Broad-sense heritability is all greater than narrow-sense heritability, that is: variation has more than 50% composition to be determined by inherent cause;
Described general combining ability height is: material is carried out to F value check between coordinate force, and the F value of general combining ability all reaches significance level;
It is to belong to Characterization that described corn inbred line is chosen basic principle;
S2: first round hybridization
Carry out incomplete diallel cross with the inbred of selecting in S1 and obtain hybrid combination, each hybrid combination is gathered in the crops separately, obtains single cross hybrid seed;
S3: second takes turns hybridization
The single cross hybrid seed of gathering in the crops in step S2 is got to equivalent and plant respectively, then carry out topcross according to the not overlapping principle of combined parent strain, by topcross combination results separately, obtain topcross kind seed;
S4: third round hybridization
By the topcross kind seed of gathering in the crops in step S3, get respectively equivalent seed and mix, then get two parts of plantations of equivalent, phase mutual cross between two parts, obtains seed, is hybrid seed one;
S5: fourth round hybridization
The hybrid seed of gathering in the crops in step S4 one is mixed, then get two parts of plantations of equivalent, phase mutual cross between two parts, obtains seed, is hybrid seed two, and hybrid seed two is mixed, and obtains corn breeding initial population.
2. synthetic method according to claim 1, is characterized in that, total plant number >=4000 of point two parts of plantations described in step S4.
3. synthetic method according to claim 1, is characterized in that, total plant number >=4000 of point two parts of plantations described in step S5.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104798681A (en) * | 2015-05-14 | 2015-07-29 | 阜阳金种子玉米研究所 | Novel summer corn SRP class group selfing line breeding method |
| CN105028175A (en) * | 2015-06-03 | 2015-11-11 | 南京市蔬菜科学研究所 | Breeding method of selfing lines of waxy corns and white waxy corns |
| CN105230470A (en) * | 2015-08-24 | 2016-01-13 | 吉林省吉阳农业科学研究院 | Establishment of dominant tester of northern spring corn |
| CN105230471A (en) * | 2015-08-24 | 2016-01-13 | 梅河口吉洋种业有限责任公司 | Establishment of dominant population of conventional sorghum |
| CN106417003A (en) * | 2016-10-12 | 2017-02-22 | 江西省农业科学院作物研究所 | Breeding method for high-yield variety of Brassica napus |
| CN106718857A (en) * | 2016-11-29 | 2017-05-31 | 云南瑞禾种业有限公司 | Efficient corn variety circulation selection |
| CN107455254B (en) * | 2017-08-10 | 2019-01-11 | 北京市农林科学院 | Corn selects system, method with the polymerization of group's major clique |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104798681A (en) * | 2015-05-14 | 2015-07-29 | 阜阳金种子玉米研究所 | Novel summer corn SRP class group selfing line breeding method |
| CN104798681B (en) * | 2015-05-14 | 2016-11-02 | 阜阳金种子玉米研究所 | A kind of breeding method of summer corn SRP monoid selfing line |
| CN105028175A (en) * | 2015-06-03 | 2015-11-11 | 南京市蔬菜科学研究所 | Breeding method of selfing lines of waxy corns and white waxy corns |
| CN105028175B (en) * | 2015-06-03 | 2018-03-09 | 南京市蔬菜科学研究所 | A kind of selection of waxy corn and white Waxy Corn Inbred Lines |
| CN105230470A (en) * | 2015-08-24 | 2016-01-13 | 吉林省吉阳农业科学研究院 | Establishment of dominant tester of northern spring corn |
| CN105230471A (en) * | 2015-08-24 | 2016-01-13 | 梅河口吉洋种业有限责任公司 | Establishment of dominant population of conventional sorghum |
| CN105230470B (en) * | 2015-08-24 | 2018-05-01 | 吉林省吉阳农业科学研究院 | The establishment of northern spring maize advantage Testers |
| CN105230471B (en) * | 2015-08-24 | 2018-06-05 | 梅河口吉洋种业有限责任公司 | The foundation of conventional sorghum dominant population |
| CN106417003A (en) * | 2016-10-12 | 2017-02-22 | 江西省农业科学院作物研究所 | Breeding method for high-yield variety of Brassica napus |
| CN106417003B (en) * | 2016-10-12 | 2018-06-26 | 江西省农业科学院作物研究所 | A kind of selection of cabbage type rape high-yield variety |
| CN106718857A (en) * | 2016-11-29 | 2017-05-31 | 云南瑞禾种业有限公司 | Efficient corn variety circulation selection |
| CN107455254B (en) * | 2017-08-10 | 2019-01-11 | 北京市农林科学院 | Corn selects system, method with the polymerization of group's major clique |
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