CN107079810A - One-hill-multiple-plant strengthens the inbred line breeding method of the resistance to close lodging resistance of corn - Google Patents
One-hill-multiple-plant strengthens the inbred line breeding method of the resistance to close lodging resistance of corn Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
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Abstract
The present invention, which provides one-hill-multiple-plant, strengthens the inbred line breeding method of the resistance to close lodging resistance of corn, comprises the following steps:A, the hybrid seed for obtaining 2 corn excellent strain hybridization are used as S0For seed, S0In generation, selects excellent strain selfing to obtain S1For seed;B, one-hill-multiple-plant high-density planting S1Generation, the individual plant selfing that male and female are coordinated reserves seed for planting before grain weight per panicle 10% selfing fruit ear per fringe;C, by head progeny row one-hill-multiple-plant high-density planting S2 generations, to selecting the individual plant that male and female are coordinated to carry out selfing in every cave of head progeny row one end five, remaining individual plant opens pollination, and triple screenings are carried out to head progeny row;D, plantation S3Generation, with it and 45 existing conventional self-mating system test crosses, test cross F1Multi-point sampler is carried out, according to strong adaptability, quality be excellent, yield general combining ability measurement result height is screened, the head progeny row selected is the resistance to close anti-fall self-mating system of corn.The method of the present invention can make the underground root system and aerial root of educated self-mating system all very flourishing, with excellent resistance to close capacity for the resistance to lodging.
Description
Technical field
The present invention relates to breeding technique, specifically a kind of one-hill-multiple-plant strengthens the inbred line breeding side of the resistance to close lodging resistance of corn
Method.
Background technology
At present, the High planting of the most of corn inbred lines of in the market, lodging resistance can not all adapt to corn mechanization process
Demand for development, lodging, the problem of falling folding are very serious.Especially root lodges, and will have a strong impact on the production of hybrid seeds of preparing hybrid kind and examines
The important step such as fixed, is the critical defect of corn inbred line.By improving seed selection density, the resistance to close anti-fall of kind can be increased
Property.But traditional resistance to close system of selection is to be uniformly distributed, focus on resistance to close selection on the ground, the soil ignored between different plants is supported
Divide competition, it is impossible to really realize the seed selection of resistance to close anti-fall material.
The problem of in order to solve current most of corn inbred line High plantings, lodging resistance difference, we manage according to genetic breeding
By with reference to breeding practice, it is proposed that a kind of new one-hill-multiple-plant strengthens the inbred line breeding method of the resistance to close lodging resistance of corn, realizes
Inbred line breeding method is again broken through.A kind of new one-hill-multiple-plant strengthens the inbred line breeding side of the resistance to close lodging resistance of corn
Method can be not only given full play to using the competition for space between different plants and ground illumination competition, can also extend downwardly into ground
Below face, soil nutrient, the water competition between different plants are realized, so as to select underground root system and aerial root is more sent out
Reach, the stronger corn inbred line of High planting lodging resistance, be a kind of selection of highly effective.
The content of the invention
The present invention, which provides a kind of one-hill-multiple-plant, strengthens the inbred line breeding method of the resistance to close lodging resistance of corn.
A kind of one-hill-multiple-plant of the present invention strengthens the inbred line breeding method of the resistance to close lodging resistance of corn, including following step
Suddenly:
Based material S is selected based on A, the hybrid seed for obtaining 2 corn excellent strain hybridization0For seed, S0In generation, selects excellent
Strain selfing obtains S1For seed;
B, one-hill-multiple-plant high-density planting S1Generation, the individual plant coordinated at the florescence to male and female carries out selfing, will be single in the maturity period
10% selfing fruit ear is individually reserved seed for planting per fringe before Ear weight, obtains S2For seed;
C, by head progeny row one-hill-multiple-plant high-density planting S2 generations, coordinate at the florescence to selecting male and female in every first five cave of head progeny row one end
Individual plant carries out selfing, and remaining individual plant opens pollination, and the maturity period carries out triple screenings to head progeny row, specific as follows:
C1, the average grain weight per panicle to open pollination individual plant filter out the heavier head progeny row of average grain weight per panicle as index;
C2, C1 selected head progeny row is using empty stalk strain ratio and the uncomfortable strain ratio sum of male and female as index, the empty stalk strain ratio of screening
The relatively low head progeny row with the uncomfortable strain ratio sum of male and female;
C3, C2 selected head progeny row is so that plant type is compact, spinning is concentrated soon, loose powder and spinning interval are short, rhizome is strong, living
The characters such as stalk maturation are that index carries out comprehensive screening, retain the head progeny row for meeting all indexs;
The selfing individual plant of each selected head progeny row is individually reserved seed for planting, S is obtained3For seed;
D, plantation S3Generation, with it and several existing conventional self-mating system test crosses, test cross F1Multi-point sampler is carried out, according to adaptation
Property strong, quality is excellent, general combining ability measurement result of yield height is screened, the head progeny row selected is the resistance to close anti-fall selfing of corn
System.
Wherein, the S0In generation, selects excellent strain selfing to obtain S1For seed, S is specially planted0More than 100 plants are obtained for seed
Plant, chooses more than 50 plants of well-grown plant bagging selfing, obtain at least more than 30 selfing fruit ear or 10000
Selfing seed above is used as S1For seed.
Wherein, the one-hill-multiple-plant is more than every 2 plants of cave (containing), preferably to be planted with every cave 2-3 plants of pattern, more excellent
The pattern for electing every 3 plants of cave as is planted.
Wherein, the high density is 8000-12000 plants/acre of planting density.
Wherein, described one end is the convenient progress pollination operation close to the one end in aisle.
Wherein, the heavier head progeny row of average grain weight per panicle is filtered out described in C1, preparation proportion is 20%.
Wherein, empty stalk strain ratio and the relatively low head progeny row of the uncomfortable strain ratio sum of male and female are screened described in C2, preparation proportion is
20%-25%, preferably 20%.
Wherein, it is that fringe portion blade and the angle of stalk are less than or equal to 30 ° that the plant type is compact.
Wherein, it is described to spin fast and be grouped as in head progeny row the different individual plants spinning phases and concentrate within 2 days.
Wherein, the loose powder and the spinning interval short loose powder phase for each individual plant are differed within 2 days with the spinning phase.
Wherein, the rhizome it is strong for the lodging of whole breeding time stem fall folding and/or root lodging fall folding sum less than 3%, it is empty
Bar rate is less than 3%.
Wherein, the straw staygreen is that wax ripeness latter stage plant leaf keeps green.
Wherein, the multiple spot is accredited as the testing site more than 15 and tested.
Wherein, the strong adaptability is that ecotope Major Diseases are reached with moderate resistance above rank resistance.
Wherein, it is seed Crude starch content >=73% that the quality is excellent.
Wherein, the general combining ability measurement result height of the yield refers to test cross F1Yield is higher than local conventional Hybrid pair
According to more than 10%.
The present invention also provides the inbred line breeding method of the one-hill-multiple-plant enhancing resistance to close lodging resistance of corn in corn variety
Application in breeding.
The beneficial effects of the invention are as follows:
A kind of inbred line breeding method of one-hill-multiple-plant enhancing resistance to close lodging resistance of corn provided by the present invention can make choosing
The underground root system and aerial root for educating self-mating system are all very flourishing, could power with excellent High planting and anti-.
It is poor that the method solves most of self-mating system High plantings, lodging resistance, especially root serious lodging the problem of so that
Substantially increase the resistance to close lodging tolerance of Breeding of Inbred Lines, it is ensured that breeding work is smoothed out.
Brief description of the drawings
Fig. 1 is the flow chart for the inbred line breeding method that one-hill-multiple-plant of the present invention strengthens the resistance to close lodging resistance of corn.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Kind capital 92, capital 724, capital MC01 are public kind involved by embodiment.
Embodiment 1
Flow according to Fig. 1 carries out the planting density comparative test of the resistance to close anti-fall inbred line breeding of corn, specific as follows:
A, selection self-mating system capital 92, the phase mutual cross of capital 2416 obtain the hybridization group of 92 × capital of capital 2416 in Beijing within 2014
Close, plantation gained hybrid seed obtains S0For 100 plants of plant, and choose well-grown 50 plants of plant bagging selfing;Obtain 30
Individual selfing fruit ear, thereon seed be used as S1For seed.
B、S1In generation, starts trait segregation, thus claims S1It is basic population on behalf of choosing.Beijing little Tang Shan breedings base according to
8000 plants/acre (per 2 plants of cave), 12000 plants/acre (per 3 plants of cave), 3 planting densities of 18000 plants/acre (per 3 plants of cave) are planted
Plant.At the florescence to S1The individual plant that male and female are coordinated in generation carries out selfing, and the maturity period harvests the fruit ear of selfing individual plant.By all harvests
The independent threshing of fruit ear, weighing, according to grain weight per panicle descending order, by the selected reservation of before ranking 10% fruit ear, as S2
For seed.8000 plants/acre, under 12000 plants/acre of planting density, 200 head progeny rows are selected in respectively;18000 plants/acre of plantation is close
Under degree, the selection pressure of high intensity causes 95% plant the incongruous empty bar phenomenon of male and female occur, is only selected in 100 head progeny rows.
C, then, the S2 of selected reservation is planted by head progeny row, with the 3 of 8000 plants/acre, 12000 plants/acre, 18000 plants/acre
The cropping pattern in individual planting density and every 3 plants of cave is planted.First five of close field aisle in the florescence is to S2 head progeny row materials
In cave, the individual plant selfing for selecting male and female to coordinate, remaining individual plant opens pollination, and the maturity period carries out triple screenings to head progeny row, specifically such as
Under:
C1, the average grain weight per panicle to open pollination individual plant filter out before average grain weight per panicle 20% head progeny row as index.
8000 plants/acre, under 12000 plants/acre of planting density, 40 head progeny rows are selected in respectively;It is high-strength under 18000 plants/acre of planting density
The selection pressure of degree causes more than 95% plant the incongruous empty bar phenomenon of male and female occur, is only selected in 5 head progeny rows.
C2, using empty stalk strain ratio and the uncomfortable strain ratio sum of male and female as index, the empty stalk strain ratio of screening and the uncomfortable strain of male and female
The relatively low preceding 20%-25% of ratio sum head progeny row.8000 plants/acre, under 12000 plants/acre of planting density, 40- is selected in respectively
50 head progeny rows;Under 18000 plants/acre of planting density, the selection pressure of high intensity causes more than 95% plant male and female occur not
The empty bar phenomenon of mediation, is only selected in 5 head progeny rows.
C3, with plant type is compact, spinning is fast and concentrates, loose powder and the characters such as interval short, rhizome is strong, straw staygreen of spinning
Comprehensive screening is carried out for index:The compact plant type is that fringe portion blade and the angle of stalk are less than or equal to 30 °.The spinning
It is fast and be grouped as in head progeny row the different individual plants spinning phases and concentrate within 2 days.The loose powder and spinning interval are short for each individual plant
The loose powder phase with spinning the phase differ within 2 days.The rhizome is strong, and for the lodging of whole breeding time stem, folding or root lodging fall to roll over always
With less than 2%.The straw staygreen is that wax ripeness latter stage plant leaf keeps green.
8000 plants/acre, under 12000 plants/acre of planting density, the head progeny row 20 for meeting all indexs is retained respectively;18000
Plant/acre planting density under, retain and meet the head progeny rows 2 of all indexs.
The selfing individual plant of each selected head progeny row is individually reserved seed for planting, S is obtained3For seed;
D, plantation S3Generation, using it as parent to be measured, with the test cross such as existing conventional self-mating system capital 724, capital MC01, DH382.
Test cross F1Multiple spot survey is carried out in 15 testing sites on the ground such as Beijing, Henan, Shandong, Shanxi, Jilin, Liaoning, Heilungkiang, Inner Mongol
Examination, according to strong adaptability, quality be excellent, yield general combining ability measurement result height is screened.The strong adaptability is to life
State area Major Diseases reach that moderate resistance above rank resistance, lodging rate are less than 3% less than 5%, empty bar rate;The excellent quality is seed
Grain Crude starch content >=73%;The general combining ability measurement result height of the yield refers to test cross F1Yield higher than control 10% with
On.The control is local conventional Hybrid.
The head progeny row that 8000 plants/acre, 12000 plants/acre, 18000 plants/acre of 3 planting densities are selected is respectively corn selfing
It is capital 92A-1, capital 92A, capital 92A-2 etc..The performance of each self-mating system is as follows:
Described corn inbred line capital 92A-1 shows as that plant type half is compact, and the angle of fringe portion blade and stalk is 30 °;Fringe
The different individual plant spinning phases concentrate on 2 days in row;Loose powder and spinning interval are long, and the loose powder phase of each individual plant differs 2 with the spinning phase
My god;Rhizome is general, almost without aerial root, and whole breeding time averagely lodges the rate of falling folding for 2-3%;Average sky bar rate is 2-3%;
Wax ripeness latter stage plant leaf keeps green;Moderate resistance base rot disease, rough dwarf disease, smut, curved born of the same parents bacterium leaf spot;It is Resistance To Helminthosporium Turcicum, small
Pinta;Combination seed Crude starch average content 73.5% is measured, the control of test cross yield result average specific is high by more than 10.0%.
The resistance to close anti-fall self-mating system capital 92A of described corn shows as that plant type is compact, and the angle of fringe portion blade and stalk is
24°;Spinning is fast and concentrates, and the different individual plant spinning phases concentrate on 1-2 days in head progeny row;Loose powder and spinning interval are short, each individual plant
The loose powder phase with spinning the phase differ 1-2 days;Rhizome is strong, and aerial root is flourishing, and the rate of falling folding that averagely lodges of whole breeding time is
1.0%;Average sky bar rate is 1.5%;Wax ripeness latter stage plant leaf keeps green;High anti-base rot disease and rough dwarf disease, anti-black powder
Disease, moderate resistance northern and southern leaf blight and curved born of the same parents bacterium leaf spot etc.;It is measured combination seed Crude starch average content 75.0%, test cross yield result
Average specific control is high by 13.0%.
Described corn inbred line capital 92A-2 shows as that plant type is compact, and the angle of fringe portion blade and stalk is 30 °;Spinning
Concentrate soon, the different individual plants spinning phases concentrate on 2 days in head progeny row;Loose powder and spinning interval it is short, the loose powder phase of each individual plant with
The spinning phase differs 2 days;Rhizome is general, and aerial root is general, and the rate of falling folding that averagely lodges of whole breeding time is 4%;Average sky bar rate is
3%;Wax ripeness latter stage plant leaf keeps green;Anti- or moderate resistance base rot disease, rough dwarf disease, anti-smut, northern and southern leaf blight, feel curved born of the same parents
Bacterium leaf spot etc.;Combination seed Crude starch average content 72.0% is measured, the control of test cross yield result average specific is high by 5% or so.
Embodiment 2
Flow according to Fig. 1 carries out the cropping pattern comparative test of the resistance to close anti-fall inbred line breeding of corn, specific as follows:
A, selection G. eurycarpa self-mating system capital 92, the phase mutual cross of capital 2416 obtain 92 × capital of capital 2416 miscellaneous in Beijing within 2014
Combination is handed over, plantation gained hybrid seed obtains S0For 100 plants of plant, and choose well-grown 50 plants of plant bagging selfing;Obtain
30 selfing fruit ears are obtained, seed is used as S thereon1For seed.
B、S1In generation, starts trait segregation, thus claims S1It is basic population on behalf of choosing.Beijing little Tang Shan breedings base according to
12000 plants/acre of planting density and every 1 plant of cave, every 3 plants of cave, every 5 plants of cave, line-spacing 55.00cm cropping pattern is planted.
At the florescence to S1The individual plant that male and female are coordinated in generation carries out selfing, and the maturity period harvests the fruit ear of selfing individual plant.By all harvest fruits
The independent threshing of fringe, weighing, according to grain weight per panicle descending order, by the selected reservation of before ranking 10% fruit ear, as S2Generation
Seed.Cropping pattern per 1 plant of cave, per 3 plants of cave is selected in 200 fruit ears respectively;Cropping pattern per 5 plants of cave causes whole plant
Male and female are uncomfortable or empty bar, do not obtain normal solid fruit ear.
C, then, 200 head progeny rows are planted into by the S2 of selected reservation by head progeny row, with 12000 plants/acre of planting density and every
1 plant of cave, every 3 plants of cave, every 5 plants of cave, line-spacing 55.00cm cropping pattern is planted.It is close in the florescence is to S2 head progeny row materials
In first five cave in field aisle, the individual plant selfing for selecting male and female to coordinate, remaining individual plant opens pollination, and the maturity period carries out three to head progeny row
Screen again, it is specific as follows:
C1, the average grain weight per panicle to open pollination individual plant filter out before average grain weight per panicle 20% head progeny row as index.
Cropping pattern per 1 plant of cave, per 3 plants of cave is selected in 40 head progeny rows respectively.
C2, using empty stalk strain ratio and the uncomfortable strain ratio sum of male and female as index, the empty stalk strain ratio of screening and the uncomfortable strain of male and female
The relatively low preceding 20%-25% of ratio sum head progeny row.Cropping pattern per 1 plant of cave, per 3 plants of cave is selected in 40-50 head progeny row respectively.
C3, with plant type is compact, spinning is fast and concentrates, loose powder and the characters such as interval short, rhizome is strong, straw staygreen of spinning
Comprehensive screening is carried out for index:The compact plant type is that fringe portion blade and the angle of stalk are less than or equal to 30 °.The spinning
It is fast and be grouped as in head progeny row the different individual plants spinning phases and concentrate within 2 days.The loose powder and spinning interval are short for each individual plant
The loose powder phase with spinning the phase differ within 2 days.The rhizome is strong, and for the lodging of whole breeding time stem, folding or root lodging fall to roll over always
With less than 2%.The straw staygreen is that wax ripeness latter stage plant leaf keeps green.
Cropping pattern per 1 plant of cave, per 3 plants of cave retains the head progeny row 20 for meeting all indexs respectively.
The selfing individual plant of each selected head progeny row is individually reserved seed for planting, S is obtained3For seed.
D, plantation S3Generation, using it as parent to be measured, with the test cross such as existing conventional self-mating system capital 724, capital MC01, DH382.
Test cross F1Multiple spot survey is carried out in 15 testing sites on the ground such as Beijing, Henan, Shandong, Shanxi, Jilin, Liaoning, Heilungkiang, Inner Mongol
Examination, according to strong adaptability, quality be excellent, yield general combining ability measurement result height is screened.The strong adaptability is to life
State area Major Diseases reach that moderate resistance above rank resistance, lodging rate are less than 3% less than 5%, empty bar rate;The excellent quality is seed
Grain Crude starch content >=73%;The general combining ability measurement result height of the yield refers to test cross F1Yield higher than control 10% with
On.The control is local conventional Hybrid.
The head progeny row that cropping pattern per 1 plant of cave, per 3 plants of cave is selected is respectively corn inbred line capital 92B-1, capital 92B etc..Respectively
The performance of individual self-mating system is as follows:
Described corn inbred line capital 92B-1 shows as that plant type is compact, and the angle of fringe portion blade and stalk is 30 °;Spinning
Concentrate soon, the different individual plants spinning phases concentrate on 2 days in head progeny row;Loose powder and spinning interval are slightly long, the loose powder phase of each individual plant
Differed 2 days with the spinning phase;Rhizome is general, and aerial root is general, and the rate of falling folding that averagely lodges of whole breeding time is more than 5%;Average sky
Bar rate is 3%;Wax ripeness latter stage plant leaf keeps green;It is anti-or moderate resistance base rot disease, rough dwarf disease, smut, northern and southern leaf blight, curved
Born of the same parents' bacterium leaf spot etc.;Combination seed Crude starch average content 72.0% is measured, test cross yield result average specific control high 9.0% is left
It is right.
The resistance to close anti-fall self-mating system capital 92B of described corn shows as that plant type is compact, and the angle of fringe portion blade and stalk is
26°;Spinning is fast and concentrates, and the different individual plant spinning phases concentrate on 1-2 days in head progeny row;Loose powder and spinning interval are short, each individual plant
The loose powder phase with spinning the phase differ 1-2 days;Rhizome is strong, and aerial root is flourishing, and the rate of falling folding that averagely lodges of whole breeding time is
2.0%;Average sky bar rate is 2.5%;Wax ripeness latter stage plant leaf keeps green;High anti-base rot disease and rough dwarf disease, anti-black powder
Disease, moderate resistance northern and southern leaf blight and curved born of the same parents bacterium leaf spot etc.;It is measured combination seed Crude starch average content 74.0%, test cross yield result
Average specific control is high by 12.0%.
Claims (10)
1. a kind of one-hill-multiple-plant strengthens the inbred line breeding method of the resistance to close lodging resistance of corn, it is characterised in that comprise the following steps:
Based material S is selected based on A, the hybrid seed for obtaining 2 corn excellent strain hybridization0For seed, S0In generation, selects excellent strain certainly
Friendship obtains S1For seed;
B, one-hill-multiple-plant high-density planting S1Generation, the individual plant coordinated at the florescence to male and female carries out selfing, in the maturity period by grain weight per panicle
Preceding 10% selfing fruit ear is individually reserved seed for planting per fringe, obtains S2For seed;
C, by head progeny row one-hill-multiple-plant high-density planting S2 generations, at the florescence to selecting the individual plant that male and female are coordinated in every first five cave of head progeny row one end
Selfing is carried out, remaining individual plant opens pollination, and the maturity period carries out triple screenings to head progeny row, specific as follows:
C1, the average grain weight per panicle to open pollination individual plant filter out the heavier head progeny row of average grain weight per panicle as index;
C2, C1 selected head progeny row screens empty stalk strain ratio and female using empty stalk strain ratio and the uncomfortable strain ratio sum of male and female as index
The relatively low head progeny row of male uncomfortable strain ratio sum;
C3, C2 selected head progeny row with plant type is compact, spinning is fast and concentrates, loose powder with spinning interval is short, rhizome is strong, live stalk into
The character such as ripe is that index carries out comprehensive screening, retains the head progeny row for meeting all indexs;
The selfing individual plant of each selected head progeny row is individually reserved seed for planting, S is obtained3For seed;
D, plantation S3Generation, with it and several existing conventional self-mating system test crosses, test cross F1Carry out multi-point sampler, according to strong adaptability,
Quality is excellent, yield general combining ability measurement result height is screened, and the head progeny row selected is the resistance to close anti-fall self-mating system of corn.
2. selection as claimed in claim 1, it is characterised in that the S0In generation, selects excellent strain selfing to obtain S1For seed, specifically
For plantation S0More than 100 plants of plant is obtained for seed, more than 50 plants of well-grown plant bagging selfing is chosen, obtains at least
The selfing fruit ear of more than 30 or the selfing seed of more than 10000 are used as S1For seed.
3. selection as claimed in claim 1, it is characterised in that the one-hill-multiple-plant is with more than 2 plants of every cave, preferably often
3 plants of cave.
4. selection as claimed in claim 1, it is characterised in that the high density is 8000-12000 plants/acre of plantation
Density.
5. selection as claimed in claim 3, it is characterised in that the high-density planting is with every mu of 4000 caves.
6. selection as claimed in claim 1, it is characterised in that the heavier fringe of average grain weight per panicle is filtered out described in C1
OK, preparation proportion is 20%;Empty stalk strain ratio and the relatively low head progeny row of the uncomfortable strain ratio sum of male and female, preparation proportion are screened described in C2
For 20%-25%.
7. selection as claimed in claim 1, it is characterised in that the spinning is fast and is grouped as in head progeny row different individual plants and tells
The silk phase was concentrated within 2 days.
8. selection as claimed in claim 1, it is characterised in that the short loose powder and spinning interval are each individual plant
The loose powder phase is differed within 2 days with the spinning phase.
9. selection as claimed in claim 1, it is characterised in that the multiple spot is accredited as the testing site more than 15 and surveyed
Examination.
10. selection as claimed in claim 1, it is characterised in that the general combining ability measurement result height of the yield refers to
Test cross F1Yield is higher than local conventional Hybrid control more than 10%.
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CN107593302A (en) * | 2017-10-10 | 2018-01-19 | 平顶山市农业科学院 | A kind of method accelerated New variety of leek and cultivate process |
CN109566397A (en) * | 2018-12-17 | 2019-04-05 | 北京市农林科学院 | Using single fruit Ear weight as the corn inbred lines method of core index |
CN115589939A (en) * | 2022-10-12 | 2023-01-13 | 新疆农垦科学院(Cn) | Breeding method of silage corn male parent |
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