CN114145225A - Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 - Google Patents
Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 Download PDFInfo
- Publication number
- CN114145225A CN114145225A CN202110825442.XA CN202110825442A CN114145225A CN 114145225 A CN114145225 A CN 114145225A CN 202110825442 A CN202110825442 A CN 202110825442A CN 114145225 A CN114145225 A CN 114145225A
- Authority
- CN
- China
- Prior art keywords
- bolting
- rape
- early
- yield
- moss
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method of early-maturing high-yield lobular wax powder-free rape bolting common fragrant moss 502. The invention selects the cytoplasmic male sterile line 118A as a female parent and the restorer line early bolting 30 as a male parent for hybridization, and obtains the new rape Changxiang moss 502 which is extremely early maturing, crisp and sweet in taste, low in cellulose content, large in bolting leaf ratio, free of wax powder in leaves and bolting stems, high in yield and good in resistance through directional screening. The normal-fragrant moss 502 new variety of the rape stems bred by the breeding method has good commodity performance, is obviously superior to the existing rape stem variety, and has strong commodity competitiveness. The new rape variety bred by the method solves the problem that the early-maturing rape seed stalk variety is not early-maturing and high-yield, obviously improves the planting benefit and promotes the development of the rape seed stalk industry.
Description
Technical Field
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method of early-maturing high-yield lobular wax powder-free rape bolting common fragrant moss 502.
Background
The rape bolts are novel bolt vegetables and have the advantages of rich nutrition, emerald green color, crisp taste, strong vegetable flavor, suitability for various cooking modes and the like. However, the problems of large leaves, few leaves and more leaves of the rape bolts and thick wax powder of the leaves and the stems generally exist in the rape bolts on the market at present, the rape bolts are far inferior to other bolt vegetables in marketability, and the development of the rape bolt industry is seriously influenced.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a breeding method of a rape bolt normal fragrant moss 502 with early maturity, high yield and small leaves and no wax powder, and the bred new variety of normal fragrant moss 502 has small leaves, few bolts and leaves, large bolt-leaf ratio and no wax powder, and can improve the market share after being listed on the market; meanwhile, the method has the characteristics of early maturity and high yield, ensures that the rape stems are listed earlier, and improves the planting benefit.
In order to achieve the purpose, the invention adopts the following technical scheme:
the breeding method of the early-maturing high-yield leaflet wax-free powder rape seed common fragrant moss 502 comprises the following steps of:
(1)F0and (3) hybridization: selecting the cytoplasmic male sterile line 118A as a female parent and the restorer line early bolting 30 as a male parent to carry out hybridization to obtain F1 generation seeds;
the cytoplasmic male sterile line 118A is obtained by hybridizing a sterile line 492A serving as a female parent and a self-selected flowering Chinese cabbage P111 with good taste, multiple branches and thin wax powder serving as a male parent and then performing continuous six-generation backcross and directional breeding;
after the P111 is the rape seed oil 730 is continuously selfed for two generations, the selected individual plants are planted in rows and directionally selected, and finally a multi-branch rape line with light green leaf color and thin wax powder is selected, namely the P111;
(2) planting the F1 generation seeds, performing combined identification, and selecting the growth period, the taste, the leaf size, the bolting leaf ratio, the regeneration capacity, the resistance and the yield, wherein the combined 118A x early bolting 30 has the characteristics of early maturity, crisp and sweet taste, low cellulose content, large bolting leaf ratio, no wax powder on leaves and bolting stems, high yield, good resistance and the like, and the combined 118A x early bolting 30 is the common fragrant moss 502 of the variety.
Further, in the step (2), the selection of the growth period is carried out by recording the sowing period, the seedling emergence period, the bud period, the bolting period and the bolting period of the main bolt; selecting the taste of the flowering Chinese cabbage by a method combining field tasting and stir-frying tasting; selecting the size of the leaves by measuring the length and width of the leaves of the stems and leaves; respectively weighing the bolting leaves and the bolting stems, calculating the bolting leaf ratio, and selecting a combination with the bolting leaf ratio more than 1; screening combinations with strong regeneration capacity by counting the number of the continuous 20-plant bolting roots of each combination; the resistance of the rape bolts is selected through investigation of disease resistance and cold resistance; and (4) selecting the yield of the rape bolts by counting the actual harvest yield of the commercial bolts.
The early bolting 30 is an extremely early-maturing brassica rapa bolting variety bred by Chengdi Tian Chengdi GmbH.
The approval serial number of the sterile line 492A is Xiang Pin approval No. 318.
The rich oil 730 is bred by the crop research institute in Hunan province, and the examined serial number is Xiang Ju oil 2008001.
Compared with the prior art, the invention has the following beneficial effects:
the normal-flavor moss 502 of the new rape seed stalk variety bred by the breeding method has good commodity performance, mainly shows small leaves, large leaf ratio of the rape seed stalk and no wax powder on the leaves and the rape seed stalk, is obviously superior to the existing rape seed stalk variety, and has stronger commodity competitiveness. The invention breeds multi-branch parents, which is the basis for breeding high-yield varieties; two parents with large genetic difference are selected for distant hybridization, and the heterosis is strong, which is the key point for breeding high-yield varieties. The new rape variety bred by the method solves the problem that the early-maturing rape seed stalk variety is not early-maturing and high-yield, obviously improves the planting benefit and promotes the development of the rape seed stalk industry.
Drawings
FIG. 1 shows the spectrum of the 118A breeding line.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
EXAMPLE 1 Breeding of cytoplasmic Male sterile line 118A
(1) Breeding targets: has crisp and sweet taste, multiple branches, strong resistance and strong combining ability.
(2) Creating breeding materials: a plant with light green leaf color, thin wax powder and multiple branches is found from a test sample variety (the variety is Feng oil 730 bred by the Hunan province crop research institute in 2004, examined number Xiang Ju oil 2008001), and the quality detection is carried out after the flowering phase bagging selfing in the same year to achieve the double-low standard through fresh taste. In autumn of 2005, the harvested seeds are planted and observed to have separation phenomenon, in spring of 2006, single plants with good taste, multiple branches and thin wax powder of the flowering Chinese cabbage are selected to be bagged for selfing, disease-free plants are harvested in the mature period, and then quality detection is carried out; in autumn of 2006, planting selected single plants in rows, and in spring of 2007, continuing to perform directional selection on each plant row; and continuously planting selected single plants in rows in autumn in 2007, selecting single plants in plant rows with more regular characters in spring in 2008, and harvesting the plants in rows in the mature period. Selecting spring 2009 and spring 2010 according to the same method; and (4) propagating the collected plant rows according to the plant lines in autumn of 2010 to obtain 8 parts of new materials.
(3) And (3) sterile line breeding: in 2011 in spring, 8 parts of new material are respectively hybridized with a sterile line 492A (Xiang Pin Ju No. 318), the combination characteristics are observed in the next year, the corresponding male parent P111 which can keep sterility and has good sterility character is selected, and the subsequent generations are backcrossed continuously. The specific breeding process comprises the following steps:
f0: 2011 spring, 492A hybridizes to P111.
F1: in spring 2012, the single plants with opened petals and degenerated stamens are selected to be backcrossed with P111, and after the plants are mature, the plants are respectively harvested and threshed.
BC1F 1: in 2013, the descendants are planted in rows, during the flowering phase, the single plants with opened petals and degenerated stamens are selected to be backcrossed with the parent books of the previous generation, and after the plants are mature, the plants are harvested and threshed.
BC2F 1: and in summer of 2013, continuing to plant the backcross progeny in rows, selecting single plants to be backcrossed with the parent numbers of the previous generation in the plant rows with the same flowering phase, opened petals, complete sterility and no micro powder in the flowering phase, investigating the outcrossing maturing rate, and selecting the backcross progeny with high outcrossing maturing rate after maturation to harvest and thresh.
BC3F 1: in 2014, in the spring, single plants are selected from the backcross progeny plant rows with regular characters, consistent flowering phase and better sterile characters and are continuously backcrossed with the parent of the previous generation, and after the plants are mature, progeny with good disease resistance, high outcross seed setting rate and excellent economic characters are selected and mixed for threshing.
BC4F 1: in summer of 2014, planting in different branches, and continuing backcross directional selection.
BC5F1 — BC6F 1: selecting according to similar methods in 2015 spring and 2016 spring.
The combined testing and matching are carried out in 2016, and a sterile line with high quality, good taste of the flowering Chinese cabbage, good sterile property, multiple branches and strong testing and matching advantages is bred in 2016, and is named as 118A.
The 118A breeding pedigree is shown in figure 1:
example 2 Breeding of Normal Moss 502
Preparing a hybrid combination by using 118A and early bolting 30 in Qinghai in 2018 in summer, carrying out combination identification in 2018, respectively recording the sowing period, the emergence period, the bud period, the bolting period and the bolting period of the main bolting of each combination, and selecting the growth period; selecting the taste of the flowering Chinese cabbage by a method combining field tasting and stir-frying tasting; selecting the size of the leaves by measuring the length and width of the leaves of the stems and leaves; respectively weighing the bolting leaves and the bolting stems, calculating the bolting leaf ratio, and selecting a combination with the bolting leaf ratio more than 1; screening combinations with strong regeneration capacity by counting the number of the continuous 20-plant bolting roots of each combination; the resistance condition of each combination is known through the investigation of disease resistance and cold resistance; and finally, counting the actual harvest yield of each combined commodity bolt. By combining the selection, the hybrid combination prepared by 118A and early bolting 30 has the advantages of early development, crisp and sweet taste, good disease and cold resistance, high yield, good commodity and the like.
In order to further understand the combination characteristics of 118A x early young rape shoots 30 (the reference test name is Chang Xiang Tai 502), a new early-maturing rape shoot variety comparison test is arranged in a test field of Chand city agriculture and forestry science research institute in 2019, 15 reference test varieties are used in total, Feng Zao 45 bred by Xinwan agriculture and forestry company in Hunan is used as a reference, a cell test is adopted in the test, the area of a cell is 20 square meters, and the cell is repeatedly designed for three times and randomly. The test results are shown in the following table, and the results show that: the normal fragrant moss 502 is extremely early-maturing, crisp and sweet in taste, low in cellulose content, high in yield, good in resistance and good in marketability. Sowing the seeds at 27 days in 9 months, picking the main young shoots for 52 days from the sowing to the flat head period, and 8 days earlier than 45 days before the control; the length of the leaf is 9.9 cm, the leaf is 3.3 cm shorter than 45 cm earlier than the control, the width of the leaf is 7.2 cm, and the leaf is 4.5 cm narrower than 45 cm earlier than the control; the young shoots and leaves are 0.14 larger than 1.19 and 45 times earlier than the control. The yield of the bolting per mu is 1276.69 kg. Compared with the control Feng, the yield is increased by 269.28 kg 45 times earlier, the yield is increased by 26.73 percent, and the advantages of commodity and yield are stronger.
TABLE 1 table of properties of Chang Xiang Lao 502
Claims (2)
1. A breeding method of early-maturing high-yield lobular wax powder-free rape bolting common fragrant moss 502 is characterized by comprising the following steps:
(1)F0and (3) hybridization: selecting the cytoplasmic male sterile line 118A as a female parent and the restorer line early bolting 30 as a male parent to carry out hybridization to obtain F1 generation seeds;
the cytoplasmic male sterile line 118A is obtained by hybridizing a sterile line 492A serving as a female parent and a self-selected flowering Chinese cabbage P111 with good taste, multiple branches and thin wax powder serving as a male parent and then performing continuous six-generation backcross and directional breeding;
after the P111 is the rape seed oil 730 is continuously selfed for two generations, the selected individual plants are planted in rows and directionally selected, and finally a multi-branch rape line with light green leaf color and thin wax powder is selected, namely the P111;
(2) planting the F1 generation seeds, performing combined identification, and selecting the growth period, the taste, the leaf size, the bolting leaf ratio, the regeneration capacity, the resistance and the yield, wherein the combined 118A multiplied early bolting 30 has the characteristics of early maturity, crisp and sweet taste, low cellulose content, large bolting leaf ratio, no wax powder on leaves and bolting stems, high yield and good resistance, and the long-scented moss 502 of the variety is obtained.
2. The breeding method of the premature high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 as claimed in claim 1, wherein in the step (2), the selection of the growth period is performed by recording the sowing period, the emergence period, the bud period, the bolting period and the bolting period of the main bolt; selecting the taste of the flowering Chinese cabbage by a method combining field tasting and stir-frying tasting; selecting the size of the leaves by measuring the length and width of the leaves of the stems and leaves; respectively weighing the bolting leaves and the bolting stems, calculating the bolting leaf ratio, and selecting a combination with the bolting leaf ratio more than 1; screening combinations with strong regeneration capacity by counting the number of the continuous 20-plant bolting roots of each combination; the resistance of the rape bolts is selected through investigation of disease resistance and cold resistance; and (4) selecting the yield of the rape bolts by counting the actual harvest yield of the commercial bolts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110825442.XA CN114145225A (en) | 2021-07-21 | 2021-07-21 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110825442.XA CN114145225A (en) | 2021-07-21 | 2021-07-21 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114145225A true CN114145225A (en) | 2022-03-08 |
Family
ID=80461787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110825442.XA Pending CN114145225A (en) | 2021-07-21 | 2021-07-21 | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114145225A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145227A (en) * | 2021-07-22 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140018028A (en) * | 2012-08-03 | 2014-02-12 | 권오하 | Brassica rapa. l plant with red color and method for breeding the same |
| CN108124762A (en) * | 2018-01-24 | 2018-06-08 | 常德市农林科学研究院 | A kind of method of chemical agent removal rape stamen |
| CN110235774A (en) * | 2019-03-18 | 2019-09-17 | 长沙丰景大地农业科技有限公司 | A method of cultivating wax powder-free cabbage type rape tender flower stalk |
| CN114145227A (en) * | 2021-07-22 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage |
| CN114793880A (en) * | 2022-01-19 | 2022-07-29 | 常德市农林科学研究院 | Breeding method of precocious high-yield high-oil high-bolting-resistance oil dual-purpose rape oil moss 928 |
-
2021
- 2021-07-21 CN CN202110825442.XA patent/CN114145225A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140018028A (en) * | 2012-08-03 | 2014-02-12 | 권오하 | Brassica rapa. l plant with red color and method for breeding the same |
| CN108124762A (en) * | 2018-01-24 | 2018-06-08 | 常德市农林科学研究院 | A kind of method of chemical agent removal rape stamen |
| CN110235774A (en) * | 2019-03-18 | 2019-09-17 | 长沙丰景大地农业科技有限公司 | A method of cultivating wax powder-free cabbage type rape tender flower stalk |
| CN114145227A (en) * | 2021-07-22 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage |
| CN114793880A (en) * | 2022-01-19 | 2022-07-29 | 常德市农林科学研究院 | Breeding method of precocious high-yield high-oil high-bolting-resistance oil dual-purpose rape oil moss 928 |
Non-Patent Citations (2)
| Title |
|---|
| X.L. ZHANG，等: ""Molecular cloning and expression of the male sterility-related CtYABBY1 gene in flowering Chinese cabbage (Brassica campestris L. ssp chinensis var. parachinensis)"", 《GENETICS AND MOLECULAR RESEARCH》, vol. 13, no. 2, pages 4336 - 4347 * |
| 邓力超，等: ""不同播期对油菜油肥1号菜薹及鲜草产量的影响"", 《现代农业科技》, no. 10, pages 43 - 44 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145227A (en) * | 2021-07-22 | 2022-03-08 | 常德市农林科学研究院 | Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1915049A2 (en) | Low pungency, long day onion | |
| US9320213B2 (en) | Low pungency, long day onion | |
| CN114223533A (en) | Breeding method of high-yield and easy-to-peel tartary buckwheat variety | |
| CN114793880A (en) | Breeding method of precocious high-yield high-oil high-bolting-resistance oil dual-purpose rape oil moss 928 | |
| CN113508749A (en) | High-oil waxy corn hybridization breeding method | |
| CN107278879B (en) | Method for cultivating nutrient-rich loose cauliflower | |
| CN105052732A (en) | Purple cauliflower hybrid seed cultivation method | |
| CN114145225A (en) | Breeding method of early-maturing high-yield leaflet wax-free powder rape bolt normal fragrant moss 502 | |
| CN115669530B (en) | Rice sorghum breeding method | |
| JP7350767B2 (en) | Chia variety called Lemborg | |
| USPP28628P3 (en) | Red watercress plant named ‘RW1’ | |
| CN114145227A (en) | Breeding method of new rape variety oil moss 929 for precocious high-yield flowering cabbage | |
| CN110073972B (en) | Breeding method of purple-leaf white-flower cabbage type hybrid rape combination | |
| CN115152625B (en) | Breeding method of early-maturing summer maize inbred line | |
| KR20130137564A (en) | New strawberry variety jukhyang and the method for breeding the same | |
| CN114875072B (en) | Method for creating germplasm of purple perilla | |
| CN109362560B (en) | Breeding method of sterile line of wild patrinia type three-line indica rice | |
| Javed et al. | Development and comparative studies of double cross tomato hybrids | |
| CN113396814B (en) | Breeding method of disease-resistant, heat-resistant, fast-growing and good-taste non-heading Chinese cabbage | |
| Lin et al. | Taxonomy, Botany and Plant Development | |
| Lin et al. | 2 Taxonomy, Botany and Plant Development | |
| Almekinders et al. | Supporting farmers in maintaining and selecting seeds of local varieties | |
| Rodríguez-Armenta et al. | Characterization of backcross blueberry populations created to introgress Vaccinium arboreum traits into southern highbush blueberry | |
| Koshy | The tapioca plant and methods for evolving improved strains for cultivation | |
| CN116584370A (en) | Creating method of green Chinese onion germplasm resources suitable for northern planting |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |