CN105052732A - Purple cauliflower hybrid seed cultivation method - Google Patents
Purple cauliflower hybrid seed cultivation method Download PDFInfo
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- CN105052732A CN105052732A CN201510625929.8A CN201510625929A CN105052732A CN 105052732 A CN105052732 A CN 105052732A CN 201510625929 A CN201510625929 A CN 201510625929A CN 105052732 A CN105052732 A CN 105052732A
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Abstract
The invention relates to the field of crop cultivation, in particular to a purple cauliflower hybrid seed cultivation method. A mediummorchid parent 97.f3-7-3-1a3 is taken as a main body parent to be respectively hybridized with purple-flower vegetable material ZiYin-1 with Europe consanguinity, variant distant related ZiPa (purple cabbage) according to a seed cultivation object design technical scheme by use of existing germplasm resources; backcross or continuous backcross is performed on the F1 generation, individual selection and autocopuation in a net chamber are performed BC1, BC2 and descendants by virtue of a pedigreen method according to the seed cultivation object, so that two stable-character and good-regularity self-incompatible lines are line-bred after 6-7 generations; the hybrid F1 generation has great heterosis and has yield which is increased by not less than 80% than those of parents, and ball flowers are purple red in color, compact, gorgeous and glossy, taste crisp, tender and sweet if being not cooked, taste delicious, tasty and refreshing if being cooked, and are named as ZIJING-1.
Description
Technical field
The present invention relates to breed of crop field, particularly a kind of breeding method of purple flower cabbage Hybrid.
Background technology
Cauliflower belongs to a mutation of Cruciferae Brassica genus brassica specie, and formal name used at school BrassicaoleraceaL.var.botrytisL., is developed by wild cabbage, and original producton location is at the Mediterranean Region in Europe.And a rising star in pale reddish brown Cai Shi cauliflower family, be that in Cruciferae breeding in recent years newly creates, its bouquet is rendered as gorgeous purple, and eating mouth feel is good.
What is more important, pale reddish brown cabbage except the vitamin C there is general cauliflower being rich in, also containing more special bioactivator---anthocyan, for alimentology with medical circle is confessed has anti-ageing, preventing tumor, the effect such as hypotensive.Adapt in the world that people are to the ever-increasing demand of natural nutrition health food, Japan, Denmark, Holland, the U.S. etc. have carried out the breeding research of pale reddish brown cabbage in succession.In order to break the monopoly position of external Seed enterprises in the high-end vegetable-seed industry market of China, promote the national market competitiveness of planting industry, we are from calendar year 2001, start the breeding research of pale reddish brown cabbage.
Brassicaceous vegetable will cultivate elite hybrid, and first will cultivate economical character is good, genotypic differences is large self incompatible line or male sterile line, this just requires original breeding material as much as possible.
And the pale reddish brown cabbage breeding in various countries at that time is also in the initial stage, add blockade on new techniques, the starting material that can collect is little:
1, purple 97f
3-7-3-1a
3, by the filial generation of common cauliflower, the self incompatible line cultivated voluntarily, occurs that purple makes a variation, forms through directive breeding.Purple in bouquet, thick, comparatively consolidation, sphere is bright and clean, heavy about 0.8kg, 75 days vegetative period, black rot resistance, and virus disease, as accompanying drawing 1;
2, purple draws-1 Denmark's material, original name Qarnet, is introduced, have European blood relationship by Henan, Zhengzhou skill kind industry.Plant experimentally performance: bouquet aubergine, thinner more loose, sphere is not bery flat, and heavy about 0.3-0.5kg, in about 135 days vegetative period, can only gather before the winter on a small quantity, can not directly utilize, as accompanying drawing 2 on producing.Obviously, be difficult to reach breeding objective by means of only two parts of materials.
Summary of the invention
For the defect that above prior art exists, the object of the invention is: the breeding method that a kind of purple flower cabbage Hybrid is provided, introduce material purple cabbage between genotypic differences very large mutation, expand the heterogeneity between progeny material, select the selfing material that economical character is excellent, further assembly goes out influential point set.
Its technical scheme of dealing with problems is:
A breeding method for purple flower cabbage Hybrid, is made up of following steps:
Step one:
With purple flower cabbage self incompatible line 97f3-7-3-1a3 in locality voluntarily seed selection for female parent, draw-1 for male parent with Europe pale reddish brown dish material purple and carry out hybrid combination, obtain F1 generation crossbreed; Next year for female parent with F1 generation crossbreed, draws-1 for male parent (recurrent parent) with purple and backcrosses, obtain backcross progeny BC1, be called for short BC1;
With the purple Pa of purple cabbage self incompatible line for female parent, middle purple flower cabbage self incompatible line 97f3-7-3-1a3 is that male parent combines, and obtains distant hybrids F1 between mutation; Next year for female parent with this F1 generation crossbreed, is that male parent (recurrent parent) backcrosses with 97f3-7-3-1a3, obtains backcross progeny BC0; For increasing the genotype of 97f3-7-3-1a3, the 3rd year is female parent with BC0 again, is that male parent backcrosses, obtains secondary backcross progeny BC2, be called for short BC2 with 97f3-7-3-1a3;
Step 2:
By BC
1plant the colony about 250 strains, according to breeding objective, about selecting fine individual plant 20 strain, 15 strains of choosing immigration winter survival in greenhouse are implanted in same community, timely cover INTERNET ROOMS when buddingging eventually, and the florescence carries out selfing by number respectively, seed presses individual plant results, and next year, kind became plant.Each generation presses pedigree method seed selection later; Through 6 generations, obtain stability self incompatible line BC
1-2-1-3N-6a, skeleton symbol is expressed as purple a
12,as accompanying drawing 3;
By BC
2plant the colony about 350 strains, according to breeding objective, about selecting fine individual plant 25 strain, choosing moves into winter survival in greenhouse 15 strain eventually, field planting is in same community, timely cover INTERNET ROOMS when buddingging, the florescence carries out selfing by number respectively, and seed presses individual plant results, next year, kind became plant, each generation presses pedigree method seed selection later, through 6 generations, obtains stability self incompatible line BC
2b
2-2-8-6-2-1, skeleton symbol is expressed as purple b
2-1, as accompanying drawing 4,5,6,7;
Step 3:
When the 4th generation, choose BC respectively
1, BC
2offspring fine individual plant carries out test cross in pairs, and test cross seed is carried out ore grade indexes test,
To the 6th generation, confirm purple a
12× purple b
2-1 is optimum combination, and its No., first generation cross-fertilize seed called after amethyst, as accompanying drawing 8.
Step 4:
With the purple a of the maternal self incompatible line of described stabilization characteristics of genetics
12b purple with male parent self incompatible line
2-1 hybridizes, and produces amethyst purple flower broccoli variety seed.
In the step one of said method, male parent purple draws-1 and shifts to an earlier date sowing in 15 days, with florescence alternation, facilitates father, maternal flower synchronization.
The purple Pa and middle purple flower cabbage test material 97f of violet cabbage test material
3-7-3-1a
3belong to Cruciferae Brassica genus brassica specie, the two is edge far away between mutation, although chromosome is all 2n=2x=18, but genotypic difference is very large, economical character is completely different, its intermolecular hybrid with middle purple flower cabbage test material continuous backcross, object is: 1, to expand and other genotypic differences backcrossed between material.2, the purple gene type of violet cabbage is obtained.3,97f
3-7-3-1a
3genotype at BC
2in account for 87.5%, more easily select the excellent strain of purple flower cabbage in offspring.
In said method step one, described change interspecific hybridization is carried the previous year and is carried out, to make overall breeding work synchronous as far as possible.
In said method step one, described purple Pa be free in ripe type purple cabbage self incompatible line, as accompanying drawing 4.
In said method step 2, isolated area is set, breeds the purple a of maternal self incompatible line at nylon wire indoors artificial cabage-type rape respectively by operational procedure
12b purple with male parent self incompatible line
2-1.
In said method step 4, maternal is 1:1 with the planting proportion of male parent, must carry out North China seeding under the condition of greenhouse protection.
Original breeding material in said method and hybridization, backcross progeny are biennial plant in North China.General in nursery on 20-July 15 in June, seedling age is planted in experimental field in about 30 days, partition number field planting.Between 10-November, bouquet grows up to, and is now the critical period of Single-plant selection, according to breeding objective roguing, and selected individual plant prescinds 75% after bouquet grows up to, and does wound antisepsis process.The tolerant minimum temperature of pale reddish brown dish examination material is-2 DEG C, and North China's winter low temperature is many below-7 DEG C, so will be transplanted into before the winter, greenhouse is warming survives the winter.Branch out spring after buddingging and isolate by nylon wire or guard on system frame, come into bloom the weak flower bud of the weak branch of strict combing, and the florescence carries out individual plant stripping flower bud selfing or hybridization according to plan, backcrosses.
Be seeded into next year from examination material and gather in the crops new test material seed, the time cycle of 330-350 days be experienced.
In said method, the selected individual plant of test material, fully will grow up at bouquet, when base portion starts loosening, selects sunny drier weather, carries out cutting ball process in 9-16 time.Slice off part and account for 75-80%, only retain bouquet avris 1-2 beads, with the consumption nutrition of branching out preventing bouquet too much.Carry out sterilization treatment with gauze pulverized limestone bag immediately after cutting ball, or with the process of 600-650ppm carbendazim, prevent wound from festering.Will be with the mulch that sticks together when being transplanted into greenhouse before the winter, soil sticks together diameter about 35cm.Wintering Period every other day temperature more than 6 DEG C will be leaked informaton cooling, when the time is 10-16.
Accompanying drawing explanation
Institute's accompanying drawing is the real photograph in field of different times test material.
Fig. 1, purple 97f
3-7-3-1a
3.
Fig. 2, purple draw-1.
Fig. 3, purple a
12.
Fig. 4, be purple Pa and purple 97f
3-7-3-1a
3the F1 generation of inter-subspecies hybrid enters roguing.
Fig. 5, be inter-subspecies hybrid BC
2field enters roguing.
Fig. 6, be inter-subspecies hybrid BC
2forth generation field enters roguing.
Fig. 7, purple b
2-1.
No. one, Fig. 8, amethyst.
Embodiment
Embodiment one
This example determines one of breeding technique scheme according to Double-directional back, and the selection of the purple a12 of self incompatible line is cultivated:
Step one:
Make not affine system 97f3-7-3-1a3 by oneself for female parent with purple cauliflower in being bred as, the pale reddish brown dish material purple introduced with Denmark draws-1 for male parent hybridizes, and obtains F1 generation hybrid; Second Year for female parent with F1 generation hybrid, draws-1 for recurrent parent with purple and backcrosses, obtain the material B C1 that backcrosses;
Step 2:
From generation to generation next, BC1 kind is planted the colony about 250 strains, when having mid-October bouquet to grow up to, carry out field selection, selected individual plant requires the full purple of bouquet, comparatively large thicker, vegetative period, 80-90 days, disease-resistant, about entering roguing 20 strain, be with Tu Tuo to move into greenhouse before winter, heel in same community, on back cover of buddingging, nylon wire is isolated;
Step 3:
Post flowering plant division selfing, select that male gynoecium is normal, bud is large, column cap is longer, the individual plant that pollen amount is larger carries out selfing, the general large flower bud selecting will to bloom after 2-3 days, with tweezers by the strip off gently of the tip of flower bud, expose column cap, the mature pollen of the flower opened with homophyletic, award on the column cap exposed, pollination flower bud number about 50, pollinating when the 8:00-16:40 of fine day, from cabage-type rape to pod angle yellow maturity, is 50-60 days, by system plant division results, dry, micelle, enter bag, numbering harvest and store; Step 4:
Above-mentioned dividing receives individual plant, and succeeding year plants into plant respectively, forms a plant group, and growing period is observed with regard to aspects such as growth potential, resistance, blade profile, plant types and recorded; Carry out observation with regard to aspects such as the size of ball, thickness, color and luster, fineness, elasticities after going out ball to record; Comprehensive whole observed result, first selects optimum plant, more therefrom selects fine individual plant; It is auxiliary for selecting a more excellent plant simultaneously, therefrom suitably selects a little excellent strain; Before winter, band sticks together immigration greenhouse, and also will detect affinity index: manual self-pollination, every strain process 80-100 flower, detects fecundity in about 35 days, average each angle fruit set seeds number be less than 1.5 show that there is higher not affine trend, retained; Eliminated more than the individual plant of 1.6.
When selection was cultivated to the 4th, 5 generation, smear the individual plant that flower measures, average each angle fruit sets seeds number can not more than 0.6, and in other words, affinity index will below 0.6.
When selection is cultivated to BC
1afterwards the 4th from generation to generation time, it is important to note that:
1, the uniformity of main body proterties is noted, if the color of such as bouquet all aubergines; Grow up to from first bouquet and reach harvesting standard to last several bouquet, the time interval more than 20 days, so that the crossbreed be made into is moderate for picking time later, can not drag seedling;
2, the material selection that enters in direction of backcrossing with another one carries out mensurations and combines, and detect the hybrid vigour situation of selfing on two different directions not affinity storeroom, the number of combinations of mensuration is that 6-10 is individual, and be called product based material, next year carries out ore grade indexes test.
When selection is cultivated to BC
1afterwards the 5th, 6 from generation to generation time, important work is:
1, carry out ore grade indexes test, determine the optimum strain in ore grade indexes test.Require that first-filial generation increases by more than 50% than Parent average yield in output;
2, determine optimum Self-Incompatibility material according to ore grade indexes result, then through selfing, Identification between plant, eliminate outstanding not, merit is accumulated, purifying, draw the self incompatible line that a proterties is stable.The excellent Self-Incompatibility formed in this example is BC
1-2-1-3N-6a, skeleton symbol is expressed as purple a12.Its major traits compares as table 1 with hybridization, the original parent that backcrosses.
Table 1, purple a12 and initial cross, backcross parent COMPARISON OF CHARACTERS
As shown in Table 1, purple a12 Self-Incompatibility ties up to merit economical character combining Parent in starting material, overcomes the deficiency of parents.In 90 days vegetative period, between parents, affinity index is 0.6, and main body proterties is stablized, and disease resistance is strong, and bouquet is thick, and aubergine is gorgeous, is excellent self incompatible line.Female parent is used as in suitable hybrid seeding afterwards.
Embodiment two
The present embodiment backcrosses in breeding technique scheme according to two-way, the purple b of another relative self incompatible line
2the selection of-1 is cultivated:
Step one:
For causing the larger Traits change between the progeny material in embodiment one, the present embodiment selects the purple Pa of mutation medium purple wild cabbage self incompatible line for maternal, with middle purple cauliflower self incompatible line 97f
3-7-3-1a
3carry out hybridization for male parent and obtain cross materials F1;
Step 2:
Due to interbreed, genotypic difference is too large, and above-mentioned F1 shows extremely strong heterosis, hybrid vigor, and bouquet is a mauve colored core, so, plant for female parent with the selected of F1 again, use 97f
3-7-3-1a
3for recurrent parent backcrosses, obtain backcross progeny BC
0, continue next year to backcross, obtain BC
2;
Step 3:
By above-mentioned BC
2plant the colony about 350 strains, growing period observes record in detail, for occurring the plant of some proterties close to pale reddish brown dish, as main separation material, entering roguing is 10-12 strain, is with the immigration greenhouse heat-insulating that sticks together to survive the winter before the winter, heel in same community, nylon wire on back cover of buddingging is isolated, Post flowering plant division selfing, and bud of selecting and remain is large, Pistil And Stamen is normal, pollen amount is many, the plant that column cap is longer, when the yellow maturity of pod angle, by be plant division results, dry, threshing, enter bag, numbering harvest and store;
Step 4:
Above-mentioned dividing receives individual plant, and succeeding year plants into plant respectively, forms a plant group, and growing period is observed with regard to aspects such as growth potential, resistance, blade profile, plant types and recorded; Carry out observation with regard to aspects such as the size of ball, thickness, color and luster, fineness, elasticities after going out ball to record, comprehensive whole observed result, first selects optimum plant, then in optimum plant, select fine individual plant; It is auxiliary for selecting a more excellent plant simultaneously, therefrom suitably selects a little excellent strain.Before winter, band sticks together immigration greenhouse, and also will detect affinity index: manual self-pollination, every strain process 80-100 flower, detects fecundity in about 35 days, average each angle fruit set seeds number be less than 1.5 show that there is higher not affine trend, retained; Eliminated more than the individual plant of 1.6.
When selection is cultivated to BC
2the the 4th, 5 generation afterwards, smear the individual plant that flower measures, average each angle fruit sets seeds number can not more than 0.6, and in other words, affinity index will below 0.6.
When selection is cultivated to BC
2afterwards the 5th, 6 from generation to generation time, except routine work, important work is:
1, carry out ore grade indexes test, determine the optimum strain in ore grade indexes test;
2, according to strain test qualification result, determine optimum Self-Incompatibility material, then through selecting good strains in the field for seed selfing, Identification between plant, selecting in excellent excellent, making that the merit of most optimum materials is accumulated further, purifying, finally form the stable self incompatible line of a proterties.The excellent Self-Incompatibility formed in this example is BC
2-b
2-2-8-6-2-1, skeleton symbol is expressed as purple b
2-1, its major traits compares as table 2 with hybridization, the original parent that backcrosses.
Table 2, purple b
2-1 with initial cross backcross parent COMPARISON OF CHARACTERS
As shown in Table 2, purple b2-1 Self-Incompatibility ties up to advantage economical character combining Parent in starting material, overcomes the deficiency of parents, especially inherits the purple gene type of purple Pa, and with 97f
3-7-3-1a
3continuity backcrosses and has abandoned the leaf-head proterties of violet cabbage in offspring's directive breeding.In 83 days vegetative period, between parents, affinity index is 0.6, and main body proterties is stablized, and disease resistance is strong, bouquet aubergine, in thick, smooth, be excellent self incompatible line.And be used as male parent in suitable crossbreed afterwards.
Embodiment three
The present embodiment, according to the two-way breeding technique scheme that backcrosses, is selected to cultivate the large self incompatible line of Traits change in two different directions, is selected the Hybrid that economical character is excellent on this basis.
Step is as follows:
Step one:
Ore grade indexes is tested:
When two-way backcross breeding technique scheme implementation to the 4th of backcross progeny from generation to generation time, now main body proterties is (as plant type, the aubergine of bouquet, the thickness of bouquet, disease resistance etc.) show out and unanimous on the whole, determination test to be carried out in time, method is the fine individual plant that will two different directions be selected, carry out hybrid combination by number, each combined hybrid 35-40 flower, results about 150, seed, selected combination next year carries out ore grade indexes test, selected number of combinations 6-10, eliminate solid few combination, next year carry out ore grade indexes test,
Ore grade indexes test is field trial, nursery on June 25,22-25 in July day field planting, duplicate rows district, the long 10m of row, line-spacing 0.55m, spacing in the rows 0.5m, every community plants 40 strains, compares with parent's (Parent), period is done in detail to observe and records, when mid-October, bouquet grew up to, recording according to observing, eliminating strain outstanding not, to select and remain 2-3 excellent strain, when bouquet is gathered, poidometer produces, and adds up, obtain mean value to each crop, for 2010 annual ore grade indexes result of the tests, table 3.
Table 3,2010 annual pale reddish brown vegetable system authentication test results
As shown in Table 3, No. 005 strain, i.e. BC
1-2-1-3N-6 × BC
2-b
2this combination of-2-8-6-2, growth is neat, and curd color is that aubergine is gorgeous, ball is thick, and the heavy 1.46kg of average single ball, cell production 58.4kg, amounts to per mu yield 3540.5kg, ranks first place in Yield compari, and disease resistance is strong, strain of attaching most importance to.In conjunction with result of the test in former years, No. 005 strain Comprehensive Traits is excellent, tentatively names as No. one, amethyst, and is further purified and optimum choice its parent.
Step 2:
Varietal yield test:
On the basis of ore grade indexes test, do varietal yield test (No. one, amethyst and its parents compare) 2011 years further.
Table 4,2011 annual amethysts varietal yield test result
From table 4 in conjunction with field observation, the growth of No. one, amethyst is neat, and disease resistance is strong, grow vigorous, hybrid vigour is strong, and plant is large, from field planting to gathering about 86 days, bouquet aubergine, gorgeous glossy, bouquet consolidation, ball is large, and ball is thick regular, the heavy 1.43kg of average single ball, amounting to 667m2 output is 3467.8kg, a12 volume increase 76.54% more purple than female parent, b2-1 volume increase 98.61% more purple than male parent.The seed that No. one, amethyst, thousand kernel weight 3.5-3.8g, seed coat is dark purple brown, and kind benevolence is puce, and bud seedling is aubergine, and be lilac after true leaf grows, background color is blue-green, and bouquet is gorgeous aubergine.
Meaning of the present invention is:
1, under the awkward situation that germ plasm resource is rare, adopt the two-way breeding method that backcrosses, cultivated the not affine system of the large parental autocopulation of Traits change, and cultivated into new type of health vegetables further---No. one, purple flower cabbage crossbreed amethyst;
2, change vegetables high-end kind of industry market in the past and, by the situation monopolized, improve national self-confidence and sci-tech innovation ability of planting industry abroad.
The parent of an above-mentioned amethyst cauliflower crossbreed, maternal purple a12, with male parent b
2-1, all have and necessarily breed quantity.
In addition to the above embodiments, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of application claims.
Claims (4)
1. a breeding method for purple flower cabbage Hybrid, is characterized in that comprising the following steps:
Step one,
With purple flower cabbage self incompatible line 97f in locality voluntarily seed selection
3-7-3-1a
3for female parent, draw-1 for male parent with Europe pale reddish brown dish material purple and carry out hybrid combination, obtain F1 generation crossbreed; Next year for female parent with F1 generation crossbreed, draws-1 for male parent with purple and backcrosses, obtain backcross progeny BC1, be called for short BC1;
With the purple Pa of purple cabbage self incompatible line for female parent, middle purple flower cabbage self incompatible line 97f
3-7-3-1a
3for male parent combines, obtain distant hybrids F1 between mutation; Next year with this F1 generation crossbreed for female parent, with 97f
3-7-3-1a
3for male parent backcrosses, obtain backcross progeny BC0; For increasing 97f
3-7-3-1a
3genotype, the 3rd year take BC0 as female parent again, with 97f
3-7-3-1a
3for male parent backcrosses, obtain secondary backcross progeny BC2, be called for short BC2;
Step 2,
BC1 kind is planted the colony about 250 strains, according to breeding objective, emphasis carries out the selection of fine individual plant when the economic characters such as curd color, size, thickness, the degree of packing fully manifest, about selected individual plant 20 strain, choosing moves into 15 strain field planting of winter survival in greenhouse in same community eventually, timely cover INTERNET ROOMS when buddingging, the florescence carries out selfing by number respectively, and seed presses individual plant results, next year, kind became plant, each generation presses pedigree method seed selection later, through 6 generations, obtains stability self incompatible line BC
1-2-1-3N-6a, skeleton symbol is expressed as purple a
12;
BC2 kind is planted the colony about 350 strains, according to breeding objective, emphasis carries out the selection of fine individual plant when the economic characters such as curd color, size, thickness, the degree of packing fully manifest, about selected individual plant 25 strain, eventually choosing moves into winter survival in greenhouse 15 strain, field planting in same community, timely cover INTERNET ROOMS when buddingging, florescence carries out selfing by number respectively, seed presses individual plant results, and next year, kind became plant, and each generation presses pedigree method seed selection later, through 6 generations, obtain stability self incompatible line BC
2b
2-2-8-6-2-1, skeleton symbol is expressed as purple b
2-1;
Step 3,
When the 4th generation, choosing BC1, BC2 offspring fine individual plant respectively and carry out test cross in pairs, test cross seed is carried out ore grade indexes test, with comparatively early checking hybrid vigour from generation to generation, to the 6th generation, confirming purple a
12× purple b
2-1 is optimum combination, its No., first generation cross-fertilize seed called after amethyst;
Step 4,
With the purple a of the maternal self incompatible line of described stabilization characteristics of genetics
12b purple with male parent self incompatible line
2-1 carries out hybridization produces amethyst purple flower broccoli variety seed.
2. the breeding method of a kind of purple flower cabbage Hybrid according to claim 1, is characterized in that: become interspecific hybridization described in step one and carry the previous year and carry out, to make overall breeding work synchronous as far as possible; Described purple Pa be free in ripe type purple cabbage self incompatible line.
3. the breeding method of a kind of purple flower cabbage Hybrid according to claim 1, is characterized in that: arrange isolated area in step 2, breeds the purple a of maternal self incompatible line respectively by operational procedure at nylon wire indoors artificial cabage-type rape
12b purple with male parent self incompatible line
2-1.
4. the breeding method of a kind of purple flower cabbage Hybrid according to claim 1, is characterized in that: maternal described in step 4 is 1:1 with the planting proportion of male parent, must carry out North China seeding under the condition of greenhouse protection.
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| CN107278879A (en) * | 2017-07-31 | 2017-10-24 | 北京市农林科学院 | A kind of breeding method of eutrophy loose type cauliflower |
| CN108362649A (en) * | 2017-12-18 | 2018-08-03 | 温州科技职业学院 | A method of the detection purity of hybrid of the color based on Cauliflower hypocotyl |
| CN110301348A (en) * | 2019-08-12 | 2019-10-08 | 浙江省农业科学院 | A kind of selection of purple loose type broccoli |
| CN110301350A (en) * | 2019-08-12 | 2019-10-08 | 浙江省农业科学院 | A kind of selection of purple pagoda cauliflower cenospecies |
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Application publication date: 20151118 |