CN105638450B - A kind of low erucic acid, the high oil-producing selection of radish - Google Patents
A kind of low erucic acid, the high oil-producing selection of radish Download PDFInfo
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- CN105638450B CN105638450B CN201610008164.8A CN201610008164A CN105638450B CN 105638450 B CN105638450 B CN 105638450B CN 201610008164 A CN201610008164 A CN 201610008164A CN 105638450 B CN105638450 B CN 105638450B
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- erucic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to oily field of plant variety breeding technology, it is related to a kind of low erucic acid, the high oil-producing selection of radish, step includes：It (1) is maternal with the wild low erucic acid oil of content of erucic acid < 5% radish horse bud flower, using the high yield of content of erucic acid > 30%, resistance, the oily use radish red egg-shaped thing of high erucic acid as male parent, florescence is to the flower stripping flower bud emasculation of maternal horse bud, it awards with the sub- pollen of male parent red eggs, hybridization obtains F1；(2) divide single-strain planting F1 and be strictly selfed to obtain F2；(3) content of erucic acid that each F2 single-strain seed is tested using gas chromatograph, selects content of erucic acid < 10%, and the excellent F2 strain of economical character is reserved seed for planting, remaining is eliminated；(4) step (2) and (3) are repeated, the oil of low erucic acid, high yield, resistance and merit is obtained after inbreeding of more generation and selection with radish good fortune come 01, deposit number is CCTCC NO：P201603.Kind of the invention reduces by 80% or more content of erucic acid than original kind.
Description
Technical field
The invention belongs to oily field of plant variety breeding technology, and in particular to a kind of low erucic acid, the high oil-producing breeding side of radish
Method.
Background technique
Oil belongs to Cruciferae Rhaphanus corn radish (Raphanus raphanistrum with radish (Oilseed Radish)
L.sp), very different from common cultivation radish, oil is annual herb with radish, and taproot is thin and delicate, unloose in meat, is bloomed
Phase is early, and seed production is low.
The content of erucic acid is unfavorable for absorption of human body between 25%~45% in general oil radish varieties fatty acid total amount.
Yunnan Wild type oil is grown in 1800 or so alpine belt of height above sea level with radish varieties " horse bud flower ", and plant is short, and resistance is poor, uncomfortable
It closes plains region and southern area is promoted, but its content of erucic acid < 5%, be a kind of excellent breeding material.Gansu local varieties
" red eggs " is up to 150kg/667m with radish yield as oil2, branch capability is strong, downy mildew resistance, and resistant to lodging is a kind of high
It produces, the oil radish varieties resource of highly resistance.
Currently, oil is commercialized with radish first is that need to solve the problems, such as low output, second is that solve the problems, such as that erucic acid is high, lead to
Cross the method for the present invention select low erucic acid, high yield, highly resistance oil with radish new varieties to development oil with radish industry, promote characteristic agriculture
The development of industry and meets the diversity that people consume edible oil and have great importance.
Summary of the invention
It is an object of the invention to overcome the deficiencies of existing technologies, a kind of low erucic acid, the high oil-producing breeding side of radish are provided
Method, the invention is simple and feasible, and the oil cultivated has the characteristics that low erucic acid and high yield with radish varieties.
Previous karyotype studies show that content of erucic acid is controlled by the additive gene of two pairs of dominant effect very littles, high erucic acid base
Because type is (EAEAEBEB), low erucic acid genotype is (eAeAeBeB), wherein the additive effect of each allele is 11-11.5%
Left and right, dominant effect is 2.5-3.0% or so, therefore can be extrapolated, and high erucic acid gene kind erucic acid should be 44~46, low mustard
Acid is 0~5 or so, meets our Observe and measure numerical value.And studies have shown that erucic acid is blastema because of control, so miscellaneous in selection
It must be maternal carry out with low erucic acid kind when handing over parent and backcrossing direction.The present invention utilizes low erucic acid kind (being) and high erucic acid
Kind (being) hybridization obtains F1, and content of erucic acid is to meet e between 25~30%AeAeBeB×EAEAEBEBThe heredity of filial generation
Mode also indicates that erucic acid character inheritance is suitble to the method for the present invention application.
To achieve the above object, the present invention takes following technical scheme：
A kind of low erucic acid, the high oil-producing selection of radish, include the following steps：
(1) (Yunnan Province Kunming vegetable is purchased from the wild low erucic acid oil of content of erucic acid < 5% radish varieties " horse bud flower "
Colza subsidiary) it is female parent, it is oily (sweet with radish varieties " red eggs " with the high yield of content of erucic acid > 30%, resistance and high erucic acid
Respectful province's Zhangye Shandan County rich people seed Co., Ltd) it is male parent, flower bud emasculation is shelled to parental varieties " horse bud flower " at the florescence,
It awards with the pollen of male parent varieties " red eggs ", F1 seed is obtained by hybridization；
(2) F1 is planted with single plant method and be strictly selfed, obtain F2 group；
(3) content of erucic acid that the single-strain seed of each F2 group is tested using gas chromatograph, selects content of erucic acid <
10%, and the excellent F2 strain of economical character is reserved seed for planting, remaining is eliminated；
(4) step (2) and (3) are repeated, after 3-5 generation selfing purifying and selection, obtains low erucic acid, high yield, resistance
It is military that the Chinese Wuhan is delivered on January 5th, 2016 come 01, Raphanus sativus L. with radish good fortune with the oil of merit
The China typical culture collection center preservation of Chinese university, deposit number are CCTCC NO：P201603.
Advantages of the present invention：
Energy quickly breeding of the present invention goes out a kind of oil radish resource of low erucic acid, and the breeding time is short, can put into section faster
It grinds in breeding.The new varieties erucic acid of breeding is low, and yield is high, and resistance is suitable as the excellent backbone male parent system of cenospecies.Make
It can significantly reduce the content of erucic acid in Radish seed oil with the low erucic acid kind that the present invention selects, erucic acid can be reduced than original kind
The characteristics of 80% or more content, greatly improves oil quality, while it is with high yield, resistance be also it China mostly
Area's plantation is taken a firm foundation.
Detailed description of the invention
Fig. 1：It is oil of the invention radish " good fortune is come 01 " breed breeding family tree.
Specific embodiment
Below with reference to embodiment, the invention will be further described.
The selection and breeding family tree of the present embodiment are shown in Fig. 1.
Maternal wild species low erucic acid oil radish varieties " horse bud flower " (are purchased from Yunnan Province Kunming vegetable seed by March, 2006
Subsidiary) and male parent high yield, high erucic acid, resistance oil with radish varieties " red eggs " (Zhangye City, Gansu Province Shandan County rich people kind
Sub- Co., Ltd) in greenhouse nursery, guarantee seedling stage nursery bed temperature is not higher than 20 DEG C for cultivation, it is not less than 5 DEG C, April
Two kinds are transplanted into breeding isolation greenhouse by bottom from nursery, and planting type is according to female parent：The row of male parent is than 1:1 ratio is one to one
Cultivation, transplanting while, carry out impurity elimination according to a conventional method.Mid-May starts the flower that begins when two kinds, first by maternal " horse bud flower "
It has bloomed piece all removals, will have been peeled off for mature bud with tweezers, removal the inside stamen (i.e. stripping flower bud emasculation) then takes corresponding
The pollen of male parent varieties " red eggs " is awarded on maternal " horse bud flower ", 100 pairs of pollination is so repeated, according to oil when harvesting by the end of June
1,100 number of oil 2..... oil is harvested and is recorded, and F1 generation erucic-acid genes type should be EAEAeBeB or eAeAEBEB at this time.2007
It cultivates 100 pairs of first familiar generation combinations March, is selfed with bagging methods, obtain F2 for seed, F2 is answered for erucic-acid genes type at this time
For EAEAeBeB, eAeAEBEB, eAeAeBeB, EAEAEBEB.In March, 2008 respectively individually plants new F2 for seed, together
When with gas chromatograph for determination content of erucic acid, filter out the i.e. genotype eAeAeBeB strain of content of erucic acid < 5%, individually stay
Kind.In March, 2009 cultivates the low erucic acid strain filtered out from F2 respectively in big Tanaka, observes its disease resistance, bolting, degeneration-resistant
Property etc. indexs, emphasis choose high yield, highly resistance strain carry out individual plant selfing reserve seed for planting, repeat it is selected above and selfing purification work extremely
2013, by the selection of 5 generations, separation, after purification low erucic acid, high oil-producing are successfully obtained with radish strain " good fortune is come 01 ".Applicant will
The strain is named as oil with radish good fortune come 01, Raphanus sativus L., and it is military that the Chinese Wuhan is delivered on January 5th, 2016
The China typical culture collection center preservation of Chinese university, deposit number are CCTCC NO：P201603.
Oil of the invention is shown in Fig. 1 with radish good fortune come 01 breeding family tree.
The low erucic acid of breeding of the present invention, high oil-producing are suitable for the plantation of China's most area with radish new varieties " good fortune is come 01 ", yellow
River northern area mid or late April can open field live broadcast, broadcast preceding deep ploughing, play Gansu Province, every 667m2 applies 150kg ternary compound fertilizer and makees base fertilizer,
Cover black film after broadcasting, generally initially spent to July by the end of June, the florescence 20 days or so, by the end of September before can gather in and finish.The Yangtze river basin and with
Open field soil at the beginning of southern area can choose 11 months is cultivated, and preceding deep ploughing is broadcast, and plays Gansu Province, and every 667m2 applies 150kg ternary compound fertilizer and makees bottom
Fertilizer covers black film after broadcasting, begin general March in next year flower, the florescence 20 days or so, can gather in finish by the end of May, not interfere second stubble crop
Cultivation.
It will implement within 2015 resulting cenospecies seed " good fortune comes 01 ", with for example common radish of radish conventional cultivation variety seeds
The kinds such as kind Duanye 13 number, Zhejiang University head, the continent Nan Pan carry out same period comparison sowing and plantation, and planting conditions and management condition are homogeneous
It together, is general planting, Routine Management obtains test result as shown in Table 1.
1 2015 years variety tests of table
| Kind | 667m2Yield/kg | Content of erucic acid/% | Downy mildew, virosis resistance |
| Good fortune comes 01 | 265 | 3.27 | By force |
| Duanye 13 number | 201 | 42.5 | It is weak |
| The continent Nan Pan | 186 | 38.6 | It is relatively strong |
| Zhejiang University head | 192 | 33.5 | By force |
* place：Gansu Zhangye；Sowing time：On April 15th, 2015；Collecting time：On August 25th, 2015
The oil cultivated of the present invention is with radish " good fortune is come 01 " kind, 667m2Average product increases production compared with the continent conventional variety Nan Pan
42.4%；Compared with Zhejiang University head's volume increase 38%, compared with Duanye 13 number volume increase 31.8%.Content of erucic acid reduces by 91.5% compared with the continent Nan Pan；Compared with Zhejiang
Greatly enhance reduction by 90.2%；92.3% is reduced compared with Duanye 13 number.In downy mildew, the resistance of virosis, " good fortune comes 01 " is shown
Strong resistance, and check variety Duanye 13 number and the continent Nan Pan show poor resistance to virosis or downy mildew." good fortune comes 01 "
Low erucic acid, high yield, resistance oil realize desired effect of the invention with radish new varieties.
Claims (1)
1. deposit number is CCTCC NO：The low erucic acid of P201603, high oil-producing use radish good fortune to come 01 as the excellent backbone of cenospecies
The purposes of male parent system.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610008164.8A CN105638450B (en) | 2016-01-07 | 2016-01-07 | A kind of low erucic acid, the high oil-producing selection of radish |
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| CN201610008164.8A CN105638450B (en) | 2016-01-07 | 2016-01-07 | A kind of low erucic acid, the high oil-producing selection of radish |
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| CN105638450A CN105638450A (en) | 2016-06-08 |
| CN105638450B true CN105638450B (en) | 2018-11-20 |
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| CN112544382A (en) * | 2020-12-09 | 2021-03-26 | 湖北莱菔子科技有限公司 | High-yield cultivation method for low-mustard-acid-oil radishes in high-altitude areas |
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| CN1214708C (en) * | 2002-09-27 | 2005-08-17 | 梅时勇 | Rapid breeding selection method of novel radish variety |
| CN102283103B (en) * | 2011-07-11 | 2013-02-13 | 镇江瑞繁农艺有限公司 | Anthocyanin-enriched radish breeding method |
| CN104221847A (en) * | 2013-06-06 | 2014-12-24 | 武汉福来农业科技有限公司 | Breeding method of novel high-oil yield Raphanus sativus. L |
| CN104303995B (en) * | 2014-09-02 | 2016-08-24 | 镇江瑞繁农艺有限公司 | A kind of breeding method of rouge radish |
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Effective date of registration: 20181009 Address after: 430070 2FD, 555 Hongshan Cultural District, Wuhan, Hubei. Applicant after: Hubei Raphani Technology Co., Ltd. Address before: 430070 A block 1288, Ming Ze mansion, Luo Shi Road, Hongshan District, Wuhan, Hubei. Applicant before: WUHAN FULAI AGRICULTURAL TECH. CO., LTD. |
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