CN104255438B - A kind of selection of forage grass type Semen sojae atricolor - Google Patents

A kind of selection of forage grass type Semen sojae atricolor Download PDF

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CN104255438B
CN104255438B CN201410527254.9A CN201410527254A CN104255438B CN 104255438 B CN104255438 B CN 104255438B CN 201410527254 A CN201410527254 A CN 201410527254A CN 104255438 B CN104255438 B CN 104255438B
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seed
individual plant
selection
yield
hybridization
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CN104255438A (en
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姜慧新
翟桂玉
曹阳
刘栋
王兆凤
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SHANDONG ANIMAL HUSBANDRY MAIN STATION
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SHANDONG ANIMAL HUSBANDRY MAIN STATION
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Abstract

The present invention relates to soybean varieties breeding technique field, selection particularly to a kind of forage grass type Semen sojae atricolor: hand over 38 for maternal with bean, wild soybean DYKSW 3 is male parent, carry out hybridization and obtain first-filial generation seed, inbreeding of more generation purification is carried out by pedigree method, select upright and that branch amount is many individual plant to reserve seed for planting during selfing, to obtain final product.The kind selected revolutionizes the growth characteristics that its male parent is wound around, vertical growth, and branch from the beginning of basal part of stem, branch quantity many, have again the characteristic that male parent leaf amount is big concurrently, and bear pods from the beginning of basal part of stem, pod is close and real, is the medium annual feeding soybean varieties of a quality height, yield, has higher feeding value.

Description

A kind of selection of forage grass type Semen sojae atricolor
Technical field
The present invention relates to soybean varieties breeding technique field, particularly to the selection of a kind of forage grass type Semen sojae atricolor.
Background technology
Leguminous forage because of its be of high nutritive value, good palatability and become the high quality grass of Livestock, be Livestock day The important component of grain composition, and China's high-quality legume forage kind is poor, East China suitable cultivation leguminous forage kind is very the fewest Very few, therefore, quality is excellent, yield is high, adaptable leguminous forage kind in cultivation, it has also become the task of top priority.
Annual wild soybean is widely distributed in the Delta Region of The Yellow River in Shandong, has that stem is soft, blade amount big, nutrition The features such as abundant, green forage yield height, good palatability, feeding value is high, disease and insect resistance and strong adaptability.But its serious winding is raw Long characteristic so that it is under unicast conditions, bottom blade is often the most withered and the most yellow because sheltering from heat or light, and causes the fall of herbage quality and yield Low, also it is unfavorable for mechanized harvest, it is difficult to large-scale production.Therefore, a kind of upright forage grass type Semen sojae atricolor of selection-breeding is as herbage, tool There is important production practical significance.
Summary of the invention
In order to solve wild soybean because serious winding characteristic causes the problem that quality and yield is low, the invention provides One utilizes the cultivated soybean vertical property, carries out selection cross with annual wild soybean, cultivates vertical growth, herbage quality The selection of the forage grass type Semen sojae atricolor of the forage grass type new soybean varieties that excellent and yield is higher.
The present invention is achieved by the following measures:
The selection of a kind of forage grass type Semen sojae atricolor comprises the following steps:
With bean friendship 38 for maternal, wild soybean DYKSW-3 is male parent, carries out artificial pollination, it is thus achieved that first-filial generation seed, plants Planting rear selfing, the independent sowing of every strain obtains second filial generation seed, plantation, and from second filial generation plant, screening is upright and branch amount is many Individual plant is reserved seed for planting, and carries out inbreeding of more generation purification, to obtain final product.
Described selection, preferably from the beginning of second filial generation, filters out upright and stem branch amount from segregating generation Individual plant more than more than 10 is reserved seed for planting.
Described selection, preferably with the yield and quality of plant nutrition body as breeding goal, from inbreeding of more generation purification After strain in select the strain that yield is high, quality is excellent to reserve seed for planting.
Described selection, preferably screening individual plant forage yield strain the highest, that crude protein content is higher than 18% is reserved seed for planting.
Described selection, preferably includes following steps:
(1) plantation first-filial generation seed, selfing, the independent sowing of every strain, obtain second filial generation seed;
(2) press individual plant branch plantation second filial generation seed, in the plant of trait segregation, upright individual plant is marked and Individually sowing, and carry out the observation of branch amount between individual plant and compare, choosing upright and that stem branch amount is higher than 10 individual plant is Hybridization three generations's seed;
(3) plantation hybridization three generations's seed, continuing to choose upright and that stem branch amount is higher than 10 individual plant is four generations of hybridization Seed;
(4) plant hybridization four generation seeds, carry out the test of full-bloom stage forage yield and nutrient content, screen individual plant forage yield The highest, the crude protein content strain higher than 18%, results hybridization five generation seeds, to obtain final product.
Described selection, preferably male parent female parent interlacing plantation.
Described selection, preferably artificial pollination use the mode of " emasculation in evening, early pollination ", and maternal the cultivated soybean selects The alabastrum that dew is purple, 4-6 point emasculation in the afternoon, with the exposed stigma of nearest blade protection, morning next day, 7-9 point took after pollen immediately Pollination.
Beneficial effects of the present invention:
1, Shandong raise Semen sojae atricolor No. 2 revolutionize its male parent be wound around growth characteristics, vertical growth, and branch open from basal part of stem Begin, branch quantity many, have again the characteristic that male parent leaf amount is big concurrently, and bear pods from the beginning of basal part of stem, pod is close and real, is a quality Annual feeding soybean varieties high, that yield is medium, has higher feeding value.Variety test shows, full-bloom stage is cut Cut green forage yield 32154 kg/hm2, hay yield 9887.4 kg/hm2
2, raising No. 2 stem and leaf of Semen sojae atricolor due to Shandong and grow in great numbers, leaf amount is big, and nutrition content is high, full-bloom stage plant, dry Middle crude protein content can reach 19.7 %, and ADF content 36.1 %, NDF content 45.6 %, and good palatability, be Herbivores High quality grass.
Detailed description of the invention
In order to be better understood from the present invention, further illustrate below in conjunction with specific embodiment.
Embodiment 1:
2006: plantation bean friendship 38, shore duty bean one, holy rich middle bean 31, the soybean varieties of fodder food 4 different ripening stages of bean With 8 different ecological type wild soybeans (WFLSW-2, WFQSW-2, WFJPW-1, ZBHPW-1, DYKSW-3, QDJSW-3, DYKYW-4, BZZYW-4), use artificial pollination mode, the sowing time see table.
Results of hybridization see table.
The first-filial generation seed of results in upper table is planted, gathers in the crops second filial generation seed, plant second filial generation seed 250 to 350, observing character, result see table.
Because bean hands over 38 to be maternal, DYKSW-3 is that the filial generation of male parent occurs in that upright and that branch amount is many individual plant, institute To select this combination to carry out further selfing purification.
" bean hand over No. 2 x of the 38(people defend 4) for female parent, wild soybean DYKSW-3 is male parent " first-filial generation seed F1.
2007: plantation first-filial generation seed F1,8 combinations are received the strain of true hybrid 10, the independent sowing of every strain altogether, obtained hybridization two For seed F2.
2008: by individual plant branch plantation second filial generation seed F2.In the plant of trait segregation, upright individual plant is carried out Labelling and independent sowing, and carry out the observation of branch amount between individual plant and compare, choose uprightly and stem branch amount is higher than 10 Individual plant is hybridization three generations seed F3.
2009: plantation F3 for seed, and continuing to choose upright and that stem branch amount is higher than 10 individual plant is four generations of hybridization Seed F4.
2010: F4 is for seed for kind, carries out the test of full-bloom stage forage yield and nutrient content, screens individual plant forage yield The highest, the crude protein content strain higher than 18%, results hybridization five generation seed F5, enter district's examination and pilot production.
2011 2013 years in Jinan, Tai'an, Wudi, the Tai Er village and Zhangqiu the strain of screening is carried out variety protection examination Test, regional testing and pilot production, it is thus achieved that the growth characteristics of multiple years, yield situation and attributional analysis data.
2013, on the basis of to test data analyzer for many years, specify its stability and grass yield, declare and raise Semen sojae atricolor for Shandong No. 2 feeding soybean varieties.
Parent is the most on the books in following documents and materials:
1. 8 different ecological type wild soybeans of male parent (WFLSW-2, WFQSW-2, WFJPW-1, ZBHPW-1, DYKSW-3, QDJSW-3, DYKYW-4, BZZYW-4) in Zhai Gui jade thesis for the doctorate, it is disclosed report, " forage grass of annual wild soybean is raw Produce performance and culture and utility technical research ", Zhai Guiyu, Agricultural University Of Nanjing, 2007, thesis for the doctorate.
2. maternal bean hands over No. 2 x of the 38(people to defend 4) it is in the news in the following references: " farmers' staff officer " 1994.4 phase 17-18 page, Summer soybean new varieties " peaceful No. 4 of mountain " Hu Xulian, Gai Cuixiang, Chen Yilan.
3. maternal shore duty bean one (day general election × " shore 89036 ") is in the news in the following references: " Semen sojae atricolor circular " 2004 the 4th No. one and cultivation technique Xu Qing Xin Lirui red Zhang Junshengs of phase page 10 new soybean varieties shore duty bean.
Shandong is raised Semen sojae atricolor No. 2 and is suitable to grow under warm and moist weather conditions, needs sufficient sunlight, and plant needs at trophophase Water, fertilizer are plentiful, require soil tight, but acid ground is unfavorable for Nodule Growth, and period of duration is about 130 d.
Shandong raise Semen sojae atricolor No. 2 revolutionize its male parent be wound around growth characteristics, vertical growth, and branch open from basal part of stem Begin, branch quantity many, have again the characteristic that male parent leaf amount is big concurrently, and bear pods from the beginning of basal part of stem, pod is close and real, is a quality Annual feeding soybean varieties high, that yield is medium, has higher feeding value.Variety test shows, full-bloom stage is cut Cut green forage yield 32154 kg/hm2, hay yield 9887.4 kg/hm2
Raising No. 2 stem and leaf of Semen sojae atricolor due to Shandong and grow in great numbers, leaf amount is big, and nutrition content is high.Full-bloom stage plant, in dry Crude protein content can reach 19.7 %, ADF content 36.1 %, NDF content 45.6 %.And good palatability, it is the excellent of Herbivores Matter forage grass.
Variety yield shows
No. 2 regional experiment of variety yield result of Semen sojae atricolor are raised in Shandong
Within 2011 to 2012, stand in the examination of Jinan district, the examination of Tai'an district is stood and the regional experiment of variety knot of Wudi herbage experimental station Fruit display, with wild soybean as check variety, cradles at full-bloom stage, and within 2011, green forage yield averagely reaches 32085.1 kg/ hm2, hay yield reaches 9572.7 kg/hm2, grass yield and contrast ratio all reach significant level;Within 2012, green forage yield is average Reach 31106.7 kg/hm2, hay yield averagely reaches 9453.0 kg/hm2, except testing site, Wudi is caused serious because of heavy rain Flooding outside flood, Jinan and examination station, Tai'an district grass yield all reach significant level.
No. 2 pilot production yield result of Semen sojae atricolor are raised in Shandong
(2) pilot production
2012 to 2013 in Wudi, the Tai Er village and Zhangqiu carry out pilot production, result of the test shows, with wild soybean For check variety, cradle at full-bloom stage, in the test data of totally 6 years of 3 testing sites, have the yield data difference of 5 years to reach aobvious Work level.Wherein, fresh grass in 2012 and Radix Glycyrrhizae average product are respectively 31299.4 kg/hm2With 9412.5 kg/hm2, comparison According to improving 10.1% and 15.3%, fresh grass in 2013 and Radix Glycyrrhizae average product are respectively 30907.1 kg/hm2With 9350.6 kg/hm2 , improve 9.4% and 12.8% than comparison.
Adaptation zone: suitably more than, middle fertility the deepest at the whole province's soil layer, in sun-drenched neutrality or alkalescence soil Growth.
Cultural technigue:
Plot: need to deep plough and rake, loosing soil is smooth.Base fertilizer to be used sufficient during whole ground, per hectare can use fertilizer 30000 Kg, or use compound fertilizer 300 kg.
Sowing: sow in spring or summer sowing.Drilling or bunch planting;Drill spacing 40~50 cm, seed level 3~5 cm, sowing Amount 30~45 kg/hm2;Bunch planting distance between rows and hills is 30~40 cm, seed level 3~5 cm, application rate 22.5~30 kg/hm2
Field management: weeding in wanting in time after emerging, determines irrigation time and number of times according to soil moisture content.Have stronger Premunition, pest and disease damage is few.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not limited by embodiment System, the change made, modifies, combines, substitutes, simplifies and all should be under other any spirit without departing from the present invention and principle Equivalence substitute mode, within being included in protection scope of the present invention.

Claims (5)

1. the selection of a forage grass type Semen sojae atricolor, it is characterised in that comprise the following steps:
With bean friendship 38 for maternal, wild soybean DYKSW-3 is male parent, carries out artificial pollination, it is thus achieved that first-filial generation seed, after plantation Selfing, the independent sowing of every strain obtains second filial generation seed, plantation, screens upright and stem branch amount 10 from second filial generation plant Above individual plant is reserved seed for planting, and carries out inbreeding of more generation purification, to obtain final product.
Selection the most according to claim 1, it is characterised in that with the yield and quality of plant nutrition body for selection-breeding mesh Mark, screens the strain that individual plant forage yield is the highest, crude protein content is higher than 18% from inbreeding of more generation strain after purification and reserves seed for planting.
Selection the most according to claim 1, it is characterised in that comprise the following steps:
(1) plantation first-filial generation seed, the independent sowing of every strain, obtain second filial generation seed;
(2) press individual plant branch plantation second filial generation seed, in the plant of trait segregation, upright individual plant is marked and individually Sowing, and carry out the observation of branch amount between individual plant and compare, choosing upright and that stem branch amount is higher than 10 individual plant is hybridization Three generations's seed;
(3) plantation hybridization three generations's seed, continues to choose upright and that stem branch amount is higher than 10 individual plant for hybridization four generation seeds;
(4) planting hybridization four generation seeds, carry out the test of full-bloom stage forage yield and nutrient content, screening individual plant forage yield is Height, the crude protein content strain higher than 18%, results hybridization five generation seeds, to obtain final product.
Selection the most according to claim 1, it is characterised in that male parent female parent interlacing is planted.
Selection the most according to claim 1, it is characterised in that artificial pollination uses the side of " emasculation in evening, early pollination " Formula, maternal the cultivated soybean selects the alabastrum that dew is purple, and 4-6 point emasculation in the afternoon, with the exposed stigma of nearest blade protection, next day Morning, 7-9 point was pollinated after taking pollen immediately.
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