CN104255445A - Selective breeding method of new spring-sowing ecological type vegetable soybean variety - Google Patents

Selective breeding method of new spring-sowing ecological type vegetable soybean variety Download PDF

Info

Publication number
CN104255445A
CN104255445A CN201410469331.XA CN201410469331A CN104255445A CN 104255445 A CN104255445 A CN 104255445A CN 201410469331 A CN201410469331 A CN 201410469331A CN 104255445 A CN104255445 A CN 104255445A
Authority
CN
China
Prior art keywords
low temperature
sowing
spring
fresh edible
edible soybean
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410469331.XA
Other languages
Chinese (zh)
Inventor
顾卫红
宋荣浩
杨红娟
朱丽华
马坤
李超汉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANGHAI CO-ELITE AGRICULTURAL SCI-TECH (GROUP) CO LTD
Shanghai Keyuan Seed Co ltd
Shanghai Academy of Agricultural Sciences
Original Assignee
SHANGHAI CO-ELITE AGRICULTURAL SCI-TECH (GROUP) CO LTD
Shanghai Keyuan Seed Co ltd
Shanghai Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANGHAI CO-ELITE AGRICULTURAL SCI-TECH (GROUP) CO LTD, Shanghai Keyuan Seed Co ltd, Shanghai Academy of Agricultural Sciences filed Critical SHANGHAI CO-ELITE AGRICULTURAL SCI-TECH (GROUP) CO LTD
Priority to CN201410469331.XA priority Critical patent/CN104255445A/en
Publication of CN104255445A publication Critical patent/CN104255445A/en
Pending legal-status Critical Current

Links

Landscapes

  • Cultivation Of Plants (AREA)

Abstract

The invention provides a selective breeding method of new spring-sowing ecological type vegetable soybean variety. The method comprises the steps of performing low-temperature resistant and weak-light resistant artificial selfing orientation systemic sieving on continuous 3-5 generations under the naturally low-temperature and weak-illumination cultivation conditions of a field for spring-sowing ecological type vegetable soybean variety with good early-maturing characters; performing sexual hybridization on sieved new vegetable soybean germplasms and vegetable soybean germplasms which are high in quality and yielding ability; performing artificial selfing orientation isolation and sieving on continuous 4-6 generations of the obtained new hybridized vegetable soybean germplasms under the naturally low-temperature and weak-illumination cultivation conditions of the field, and performing enhanced stress resistance sieving on the isolated and sieved target monophyly by the aid of an indoor artificial constant low-temperature weak-illumination identification technology so as to obtain a new early-maturing, high-quality, high-yield vegetable soybean variety with strong resistance to low temperature and weak light.

Description

A kind of selection of environmental Fresh edible soybean new varieties of sowing in spring
Technical field
The invention belongs to crop breeding technical field, relate to a kind of selection of environmental Fresh edible soybean new varieties of sowing in spring specifically.
Background technology
Fresh edible soybean (vegetable soybean) is commonly called as green soy bean, is bulging grain full seed in latter stage and not yet aging, adopts blue or green edible specialized soybean kind when pod color, seed look emerald green.Fresh edible soybean is the traditional characteristics vegetables of south China Area Inhabitants eating, because it is nutritious, the unique deliciousness of mouthfeel, develop into the key farm products that China's export is earned foreign exchange in recent years, area under cultivation expands year by year, only area under cultivation just reaches about 100,000 mu District of Shanghai year, and the provinces such as Zhejiang, Jiangsu, Anhui and Fujian year area under cultivation all more than 500,000 mu, it is in adjustment agricultural planting industry structure, improve farmers' income, increase agricultural benefit aspect and to play a part and important.
In China's traditional cultivation farming system, according to the difference of seedtime, usually again Fresh edible soybean (green soy bean) kind is divided into environmental and environmental two kinds of summer sowing of sowing in spring, wherein, the cultivation of environmental Fresh edible soybean of sowing in spring is produced and is mainly distributed on the south China the Changjiang river and In The Middle And Lower Reaches.Due to environmental Fresh edible soybean of sowing in spring launch early, damage by disease and insect is few, product are of fine quality, safety is high, the dark favor by consumer, the Fresh edible soybean product that market price produces than summer sowing cultivation exceeds 2 ~ 3 times, and economic benefit is also significantly higher than summer sowing cultivation Fresh edible soybean.Nearly ten years, along with the development of China's Protected Production of Vegetables technology, the sow in spring production development of precocious facility cultivation Fresh edible soybean of on the south China the Changjiang river and In The Middle And Lower Reaches is very rapid, and defines the Changjiang river areas to the south of China precocious facility cultivation Fresh edible soybean of sowing in spring gradually and produce the dominant area band of plantation.But owing to lacking special precocity, high-quality, degeneration-resistant (low temperature resistant low light tolerance) Fresh edible soybean new varieties of precocious Protectorate cultivation of sowing in spring, greatly have impact on this area and to sow in spring the stable development that precocious Protectorate cultivation Fresh edible soybean produces.
For a long time, spring sowing cultivation Fresh edible soybean in the Changjiang river areas to the south of China is based on conventional variety, the bad climate conditions such as the consecutive low temperature (5 ~ 15 DEG C) had because this area early spring (March early and middle ten days-April early and middle ten days) is used and continuously wet weather, under early spring Protected Cultivation, conventional Fresh edible soybean kind after planting often occur that seed is not emerged (serious rotten kind) or emerge slowly, grow and the phenomenon such as bad that bears pods, cause Fresh pod yield low, economic benefit declines.Secondly, because the breeding time of conventional variety is partially late, the listing phase of product also postpones, and have influence on this area further and to sow in spring the economic benefit that precocious facility cultivation Fresh edible soybean produces, this becomes the main limiting factor of restriction southern area of China spring sowing type precocious facility cultivation Fresh edible soybean production development.Therefore, to sow in spring the tolerance to low temperature and weak light difference of environmental Fresh edible soybean kind and the present situation partially grown breeding time for this area's routine, the tolerance to low temperature and weak light that innovation seed selection is applicable to southern area facility cultivation in early spring has by force Fresh edible soybean new germ plasm or the new varieties of precocious high-quality proterties concurrently, is the key addressed this problem.
Summary of the invention
The invention provides a kind of selection of environmental Fresh edible soybean new varieties of sowing in spring, the party's ratio juris is:
District of Shanghai is utilized to be used to the consecutive low temperature low-light ecoclimatic condition had early spring, adopt the ealy maturing culture technology of plastic house, the low temperature resistant of continuous 3-5 generation and weak light resistance land for growing field crops natural selection qualification are carried out to the environmental Fresh edible soybean kind of the spring sowing with precocious high-quality proterties, low temperature resistant and the low light tolerance individual plant selfing of getting off of therefrom selecting and remain expand numerous after, by with there is high-quality, the Fresh edible soybean kind matter of high yield or disease resistance trait carries out sexual hybridization, relend the dual authenticate technology helping indoors artificial constant low temperature low-light authenticate technology and land for growing field crops natural low temperature low-light authenticate technology, the selection step by step separation offspring being carried out to objective trait is eliminated in enormous quantities, the precocity that the low temperature resistant and weak light resistance therefrom selected and remain is strong, high-quality, high yield or disease-resistant Fresh edible soybean new varieties.
Specifically, a kind of selection of sowing in spring environmental Fresh edible soybean kind of the present invention is:
Under the natural low temperature low-light condition of land for growing field crops, the low temperature resistant of continuous 3-5 generation and weak light resistance evaluation and screening are carried out to the environmental Fresh edible soybean kind of the spring sowing with precocious high-quality proterties;
Screen the Fresh edible soybean kind matter that obtains again with there is high-quality, the spring sowing type Fresh edible soybean kind matter of high yield struture carries out sexual hybridization;
Artificial autocopulation's directional separation that the hybridization Fresh edible soybean kind matter obtained carries out continuous 4-6 generation again under land for growing field crops natural low temperature and low-light condition is selected, and can obtain low temperature resistant and that weak light resistance is strong precocity, high-quality, high yield or disease-resistant Fresh edible soybean new varieties.
What wherein have the environmental Fresh edible soybean varities practice of spring sowing of precocious high-quality proterties is " blue or green crisp No. two ";
Wherein carry out low temperature resistant and weak light resistance land for growing field crops Natural Selection condition to refer at Jiangsu-zhejiang Shanghai Area one with the used consecutive low temperature (now temperature is generally 0-15 DEG C round the clock) that has and the natural low-light ecoclimatic condition that causes of continuous rainy weather in period in early spring (2-4 month);
Wherein have high-quality, high yield struture spring sowing type Fresh edible soybean kind matter application be " Taiwan 75-1 ".
In particular, the selection of a kind of environmental Fresh edible soybean kind of sowing in spring of the present invention, comprises the steps:
(1) screening of low temperature resistant low light tolerance kind and Superior line thereof:
At Jingxi-Zhejiang-Shanghai area, during annual February 15 to March 1, to sow in spring environmental soybean germplasm, sowing in every 5 days is a collection of, (6 meters across type to be seeded in the big-and-middle canopy being provided with insect protected separation net in three batches, 8 meters across type, interlock steelframe booth) or glass greenhouse in, before sowing, every mu applies 30 kilograms of nitrogen-phosphorus-potassium compound fertilizers and makes base fertilizer, again soil deep tillage is carefully harrowed, make little high furrow, furrow are high 20 centimetres, furrow face width about 250 centimetres, adopt bunch planting way, every bunch planting kind 3, the dark 2-3 centimetre in cave, cave is apart from 25 centimetres, line-spacing about 30 centimetres, the after planting fine earth of cave upper cover 1.5-2.0 cm thick, and in little high furrow surface coverage one deck mulch film, surrounding compresses, be incubated with water conservation.After seed sprouting, surperficial mulch film is needled, exposes seedling, and at booth top cover one deck sunshade net, booth surrounding is opened simultaneously and carry out aeration-cooling control booth Nei Wen and be no more than 25 DEG C, the subnormal 300 μm of ol/ (m of light intensity 2s), other management are same as routine.Whole growth and development stage carries out classification to the emergence rate of each kind, growth potential, breeding time, quality and yield traits and investigates, therefrom select and remain that emergence rate is high, growth potential is strong, breeding time is short, quality better and the high kind of output and Superior line thereof, carry out selfing respectively to reserve seed for planting, therefrom the low temperature resistant low light tolerance kind of primary election and Superior line thereof;
(2) to the environmental Fresh edible soybean kind of the spring sowing with precocious high-quality proterties just elected, under above-mentioned same field cultivating condition, the low temperature resistant of continuous 3-5 generation and weak light resistance Natural Selection is carried out:
(natural temperature and light is utilized according to condition) under above-mentioned same field cultivating condition, through M8003 line and the selfing purifying of the natural tolerance to low temperature and weak light in continuous 3-5 generation, preferably determine the target variety that low temperature resistant and weak light resistance is strong and elite seedling;
The Superior line obtained can be applied following indoor appraising method and carry out identifying and screening: (this refers under the steady temperature and illumination condition of the complete manual control in indoor)
The tolerance to low temperature and weak light authentication method in indoor seedling stage of Fresh edible soybean, namely in germination period germinating box, constant low temperature treatment conditions are 12 DEG C, and after emerging, low temperature and poor light treatment conditions are: light intensity 100 ~ 150 μm of ol/ (m 2s), room temperature 15 DEG C.Under constant low temperature 12 DEG C for the treatment of conditions of setting, soya seeds is seeded in and is equipped with in 50 hole dishes of grow seedling nutritional matrix, seedling medium should be watered sufficient moisture before sowing, every bunch planting kind 3 seeds, the after planting moistening matrix of surface cover about 1.5 cm thick, again in surface cover one deck mulch film, to keep the moisture of matrix.Cave dish is placed in vernalization under the cryogenic conditions of 12 DEG C of setting.After emerging, seedling dislocation is treated to 80 ~ 100 μm of ol/ (m in light temperature 2s), 7-10 days is cultivated under the condition of 15 DEG C, the seedling that health of therefrom selecting and remain survives, transplant planting is in land for growing field crops again, and field production technology carries out production management to these seedling survived routinely, continue to investigate the growth under the nature Thermo-Photo sensitive of land for growing field crops of each preferred monosystem of qualification and individual plant and the situation that bears pods, therefrom further strong, the many monosystems that bears pods of preferred growth gesture and individual plant selfing are reserved seed for planting simultaneously.
(3) screen the Fresh edible soybean monosystem that obtains or individual plant again with there is high-quality, the spring sowing type Fresh edible soybean kind matter of high yield struture carries out sexual hybridization:
In District of Shanghai, during being sowed in spring March 20 to March 25, autumn sowing August 25 to September 5 during, adopt plastic mulching direct sowing culture mode, low temperature resistant and the low light tolerance soybean germplasm filtered out and the germplasm materials with high-quality, high yield or the different excellent economical character such as disease-resistant are seeded in 6 meters respectively across in type standard plastic shed soil or in flowerpot, artificial hybridization is carried out flowering stage, the F1 generation seed obtained continues to plant in plastic shed soil or in flowerpot, carry out the selfing of individual plant bagging flowering stage to reserve seed for planting, a point individual plant is gathered and is obtained F2 for segregation population seed.
(4) the Fresh edible soybean segregation population seed obtained, again under field cultivating condition, carries out the natural separation screening in continuous 4-6 generation, namely obtains the Fresh edible soybean new varieties of low temperature resistant and that weak light resistance is strong precocity, high-quality, high yield:
Begin from F2 generation, during Jingxi-Zhejiang-Shanghai area was February 15 to March 5, by the F2 of acquisition for segregation population seed in three batches (a collection of weekly), adopt plastic mulching direct sowing culture mode, seed is planted in batches and is provided with in the big-and-middle canopy soil of insect protected separation net.Adopt little high furrow bunch planting way, every bunch planting kind 1 seed, cave, apart from 10-15 centimetre, line-spacing 25-30 centimetre, is after planting covered fine earth and mulch film, is carried out water conservation insulation.After seed sprouting, surperficial mulch film is needled, exposes seedling, make its self-sow.Growing period investigates each F2 for the growth potential of segregation population individual plant, the Other Main Agronomic Characters such as habit of growth and breeding time, the individual plant that fresh pod divides individual plant to investigate each segregative line picking time again bear pods number, individual plant pod is heavy, pod is long, pod is wide and the commodity property such as hundred fresh weights, therefrom the excellent list of final election divides individual plant to reserve seed for planting, and obtains F3 for preferable separate system seed.
Early spring next year, by obtain F3 for Superior line seed sowing under same natural planting and selecting pressure condition, continue to be separated to it investigation that progeny population carries out the Other Main Agronomic Characters such as plant height, branch amount, flowering stage, picking time, single-strain legumen number, individual plant pod is heavy, pod is long, pod is wide, hundred fresh weights and fresh grain quality, therefrom the excellent list of final election divides individual plant to reserve seed for planting, and obtains F4 for preferable separate system seed.Begin from F4 generation, lid layer sunshade net is added at booth top after the seed sprouting of each preferable separate system, the progeny population of each segregative line is carried out to the strengthening screening of weak light resistance, low light tolerance through continuous 3 generations has plant height concurrently, branch amount, flowering stage, picking time, single-strain legumen number, individual plant pod is heavy, pod is long, pod is wide, being separated to selfing of the Other Main Agronomic Characters such as hundred fresh weights and fresh grain quality is selected, obtain F7 for preferred monosystem, a large amount of selections again through indoor tolerance to low temperature and weak light in seedling stage are eliminated, further screening obtains precocious, high-quality, tolerance to low temperature and weak light Fresh edible soybean selected objective target monosystem.
The Fresh edible soybean new lines that selection of the present invention is bred as has following features:
● strong stress resistance: many pilots investigate qualification result, these serial new varieties to south continuous print low temperature early spring and the abnormal climate condition such as how overcast and rainy low-light resistance is significantly better than platform 292, Shen is fresh, Zhejiang is fresh and the similar Fresh edible soybean kind such as fresh of reviving, in the normal time, the Fresh edible soybean kind that the present invention is bred as, adopt spring sowing plastic house precocity to plant to accompany, not only emerge neat, and in early spring booth low temperature and poor light according under condition, plant energy normal upright grows, and there will not be excessive growth, lodges and climbs rattan phenomenon.
● prematurity is remarkable: the present invention is on the evaluation and screening basis of strengthening kind tolerance to low temperature and weak light, highlight the precocial selection of kind, therefore, no matter be that spring sowing precocious covering cultivation is accompanied or conventional spring sowing outdoor cropping, the Fresh edible soybean new varieties that the present invention is bred as are all than platform 292, Shen is fresh, Zhejiang is fresh and the similar Fresh edible soybean kind such as fresh of reviving early goes public 5-7 days, fresh pod market price is high, remarkable in economical benefits.
● quality better: the present invention is for Parental Germplasms with the special Fresh edible soybean kind of routine spring sowing type high-quality, carry out the dual evaluation and screening of resistance on this basis, therefore, the new varieties of incubation maintain the soft glutinous high-quality characteristic of easily hotting plate of fresh seed of tailored version Fresh edible soybean.How to plant experimentally qualification to show, not hardening after the fresh beans refrigeration of new varieties, very easily hot plate, mouthfeel is soft glutinous micro-sweet, and quality and flavor is splendid.
● plant and accompany wide adaptability: the present invention's research carries out under the abnormal adverse weather conditions such as the consecutive low temperature had, how overcast and rainy low-light and cold in the late spring are used to early spring in District of Shanghai, has typical representative to the weather in early spring of on the south China the Changjiang river and In The Middle And Lower Reaches.Multiple spot district examination qualification result, the new varieties of incubation have to plant widely accompanies adaptability.
Advantage of the present invention and feature:
The present invention to be used to the typical abnormal climates such as the consecutive low temperature that has and how overcast and rainy low-light early spring for implementing base condition with District of Shanghai, research establishes has practice and application representational land for growing field crops natural pressure selection screening technique, simultaneously by climatic cabinate equipment, research establishes artificial constant low temperature low-light strengthening in indoor seedling stage triage techniques, Fresh edible soybean variety source is carried out to the evaluation and screening of dual resistance, avoid simple land for growing field crops natural pressure is selected and indoors artificial constant pressure is selected lability and non-representativeness, ensure that resistance the selection result accurately and reliably.
The present invention adopts dual resistance triage techniques, the evaluation and screening in continuous 8 generations more than is carried out to spring sowing type soybean resource and breeds, the spring sowing type with objective trait obtained, Early maturity, high-quality, degeneration-resistant (tolerance to low temperature and weak light) Fresh edible soybean new germ plasm, and with it for core parent lines, by with there is high-quality, the Mid-late ripening Fresh edible soybean variety source of high yield struture carries out sexual hybridization, make tolerance to low temperature and weak light and high-quality (the large grain of large pod), yielding ability (many pods) character gene is recombinated, further by dual resistance authenticate technology of the present invention, be separated offspring to hybridization carry out the separation of artificial autocopulation's orientation system and select in a large number step by step to eliminate, be bred as spring sowing type, tolerance to low temperature and weak light has high-quality concurrently by force, the Fresh edible soybean new lines of high yield struture, enrich the species bank of southern spring sowing type Fresh edible soybean.
The spring sowing land for growing field crops natural pressure system of selection that the present invention sets up, in conjunction with artificial constant pressure selection technique in indoor seedling stage, have conveniently, easy to operate, can control and low cost, and practical application is strong, can on the south the Changjiang river and In The Middle And Lower Reaches difference implement base, Soybean Germplasm is carried out to the evaluation and screening in enormous quantities of resistance, and offspring is separated with selfing to hybridization carries out a large amount of selections and eliminate, be conducive to screening the compound new germ plasm that acquisition strong stress resistance has precocity, high-quality, high yielding character concurrently.Current domestic incubation spring sowing type Fresh edible soybean kind mainly based on high-quality, get bumper crops, be mostly applicable on the south the Changjiang river and the conventional spring sowing outdoor cropping in In The Middle And Lower Reaches weather conditions, be not suitable for spring sowing Early maturity and precocious culture underglass.
Accompanying drawing explanation
Fig. 1 is the systematic breeding family tree that 98-11-9 is selected in Shanghai
Fig. 2 is the systematic breeding family tree that 23-9-7 is selected in Shanghai
Embodiment
Below in conjunction with Fig. 1, Fig. 2, describe specific embodiment of the invention item and techniques in detail by embodiment.
Embodiment one
In District of Shanghai, during February 12 to March 1, sowing in every 5 days is a collection of, in three batches will the seed interval sowing of " blue or green shortcake No. two " (Academy of Agricultural Sciences, Shanghai City) in the big-and-middle canopy being provided with insect protected separation net or greenhouse, adopt little high furrow bunch planting planting type, every bunch planting kind 3, cave distance 25 centimetres, line-spacing 35 centimetres, after planting cover fine earth, and adding lid layer mulch film and sunshade net on little high furrow surface, in control booth, soil temperature at night is at 8 DEG C-10 DEG C, and daytime, temperature was at 12 DEG C-15 DEG C.About 12-15 days, seed starts to emerge, and needles mulch film and exposes seedling, makes its self-sow, is added a cover by sunshade net at booth top simultaneously, and in control canopy, light intensity is lower than normal light intensity.Growth of seedling is after one week, and the seedling of healthy growth of therefrom selecting and remain, every cave retains 1 strain, and other management are same as production.Whole growth and development stage investigates the growth potential of each Superior line, pod bearing period of blooming and fresh pod commodity, therefrom preferably obtain tolerance to low temperature and weak light further stronger, bear pods many individual plants, and point individual plant mixes receipts after collecting seed, the seed of mixed receipts is carried out the booth autumn delay add generation expand numerous, the lower temperature resistance of each individual plant Later growth of further observation and yield traits, therefrom further preferably strong stress resistance, bear pods many individual plants, after the mixed receipts of seed, continue to carry out tolerance to low temperature and weak light evaluation and screening to these mixed individual plants of receiving under same cultivation season and cultivation condition spring in Second Year, after the naturally low temperature resistant weak light resistance qualification in the land for growing field crops in continuous 5 generations, final election goes out strong stress resistance and has precocity concurrently, high-quality, the single-strain seed of high yield struture is mixed to be received.
The tolerance to low temperature and weak light individual plant obtained through continuous 5 generations land for growing field crops natural selection qualification is mixed the seed of receipts, further employing artificial tolerance to low temperature and weak light technology in indoor seedling stage carries out strengthening evaluation and screening (evaluation and screening method be same as before), therefrom to select and remain the individual plant of wheat seeding strong stress resistance, transplant behind land for growing field crops, culture technique manages routinely, whole growth and development stage investigates the growth potential of each individual plant further, resistance and bearing pods property, growth potential of therefrom selecting and remain by force (branch more than 2), plant type is upright (low temperature resistant, weak light resistance is strong), many pods (individual plant bears pods more than 20), after the mixed receipts of single-strain seed of standard pod rate high (2-3 grain pod ratio is greater than 70%), continue the Double Selection seed of mixed receipts being carried out to artificial constant pressure selection in indoor seedling stage and the selection of land for growing field crops natural pressure, dual step by step qualification through continuous 2 generations is eliminated with a large amount of selection, the monosystem of final election is as tolerance to low temperature and weak light Fresh edible soybean new lines, this new lines submits preservation to, depositary institution: China typical culture collection center (address: Wuhan University), preserving number is CCTCC NO:P201405, preservation date is on June 5th, 2014, Classification And Nomenclature: pulse family Glycine the cultivated soybean kind Glycine max (L) Merr. soya seeds Shanghai VS98-11-9.
According to germplasm innovation breeding goal (spring sowing type, degeneration-resistant, high-quality, high yield), in District of Shanghai, during August 25 to September 1, adopt the autumn to delay big-and-middle canopy to cover or greenhouse culture mode, by the spring sowing type, Early maturity, high-quality, degeneration-resistant (tolerance to low temperature and weak light) the Fresh edible soybean new lines Shanghai 98-11-9 that obtain through dual evaluation and screening, and spring sowing type, Mid-late ripening, high-quality, high yield, not low temperature resistant Fresh edible soybean kind Taiwan 75-1 that Taiwan is introduced, interval sowing is in big-and-middle canopy soil or flowerpot.Because more precocious than Taiwan 75-1 about 15 days of the breeding time of spring sowing of 98-11-9 is selected in Shanghai, for ensureing flower synchronization, be conducive to the carrying out of artificial hybridization pollination work, simultaneously according to the effect of autumn sowing short-day, should by Taiwan 75-1 5 days in advance and sowing in 7 days respectively.Before sowing, water at the bottom of soil is watered foot, adopt two grain sowing, every cave retains 1 young plant, guarantees that it is nourished and grown.Flowering stage respectively with Shanghai 98-11-9 and Taiwan 75-1 for Parent, carry out positive and negative artificial sexual hybridization, positive and negative F1 generation seed is mixed respectively to be received.During spring in March 5 to the March 15 of next year, respectively the F1 generation of gathering being mixed sowing is seeded in the plastic shed soil being provided with insect protected separation net, adopt conventional cultivation mode to carry out field management after emerging, the maturing stage gathers respectively and obtains F2 for segregation population seed.
During spring next year in February 12 to March 1, mixed for the hybridization F2 generation of acquisition of gathering sowing is seeded in three batches in the big-and-middle canopy or greenhouse being provided with insect protected separation net, adopt little high furrow single-seed drilling mode, spacing in the rows 15-20 centimetre, line-spacing 25-30 centimetre, after planting earthing, and at little high furrow surface cover one deck mulch film and one deck sunshade net, needle mulch film after emerging and expose seedling, and sunshade net is moved lid to booth top, control light intensity lower than the normal light intensity in outside.Other management are same as conventional production.The proterties such as flowering stage, plant type, fine hair look, pattern of each segregative line is instituted an inquiry in the plant blossom phase, investigate the pod bearing habit of each segregative line, branch amount, single-strain legumen number picking time in fresh pod, individual plant pod is heavy, pod is long, pod is wide and fresh beans quality etc., and degeneration-resistant, the high yield of selecting and remain, high-quality individual plant reserves seed for planting, and obtains hybridization F3 for segregation population seed.F3 is adopted for segregation population seed precocious culture underglass technology of sowing in spring, carry out the land for growing field crops natural pressure M8003 line in 5 generations continuously, and be greater than 72% by spring sowing type, degeneration-resistant (low temperature resistant, low light tolerance), the large large grain of pod, high-quality (fresh beans is soft glutinous micro-sweet), Early-mid ripening (time of infertility is less than 85 days), many pods (individual plant bears pods more than 25), 2-3 grain pod rate, pod hair canescence, the light green direction being greater than 35 grams to green, dry seed 100-grain weight of fresh pod, the directed selfing system of point monosystem individual plant is separated to be selected.Mixed sowing obtaining Superior line is withheld in F8.
Again mixed sowing is adopted and manually identify seedling stage and the dual tolerance to low temperature and weak light identification of means that natural selection pressure in land for growing field crops is selected, carry out the directed selfing separation screening in continuous 2 generations, final election target monosystem, and submit preservation to, depositary institution: China typical culture collection center (address: Wuhan University), preservation date: on June 5th, 2014, preserving number is CCTCC NO:P201404, Classification And Nomenclature: pulse family Glycine the cultivated soybean kind Glycine max (L) Merr. soya seeds Shanghai VS23-9-7, through ore grade indexes test and multiple spot district examination variety comparative test, it is strong that this new lines not only shows tolerance to low temperature and weak light, fresh pod commodity is good, output is high, product are of fine quality, and sowing time, elasticity was larger, cultivation adaptability is wider.
Similar with other to Shanghai VS23-9-7, Shanghai VS98-11-9 Fresh edible soybean is carried out resistance contrast, the results are shown in Table 1.
It is as follows that the resistance of table 1 and similar Fresh edible soybean kind and cultivation accompany adaptability parameter to contrast:

Claims (1)

1. a selection for environmental Fresh edible soybean new varieties of sowing in spring, is characterized in that this selection comprises the steps:
(1) under land for growing field crops natural low temperature and low-light condition, the low temperature resistant of continuous 3-5 generation and weak light resistance evaluation and screening are carried out to the environmental Fresh edible soybean kind of the spring sowing with precocious high-quality proterties;
(2) screen the Fresh edible soybean new germ plasm that obtains again with there is high-quality, the Fresh edible soybean kind matter of high yield struture carries out sexual hybridization;
(3) the hybridization Fresh edible soybean new germ plasm obtained is again under land for growing field crops natural low temperature and low-light cultivation condition, carry out the low temperature resistant of continuous 4-6 generation and the screening of weak light resistance artificial autocopulation directional separation, the target monosystem obtained is screened through the strengthening of indoors artificial constant low temperature low-light authenticate technology again, can obtain low temperature resistant and that weak light resistance is strong precocity, high-quality, high yield Fresh edible soybean new varieties.
CN201410469331.XA 2014-09-15 2014-09-15 Selective breeding method of new spring-sowing ecological type vegetable soybean variety Pending CN104255445A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410469331.XA CN104255445A (en) 2014-09-15 2014-09-15 Selective breeding method of new spring-sowing ecological type vegetable soybean variety

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410469331.XA CN104255445A (en) 2014-09-15 2014-09-15 Selective breeding method of new spring-sowing ecological type vegetable soybean variety

Publications (1)

Publication Number Publication Date
CN104255445A true CN104255445A (en) 2015-01-07

Family

ID=52147133

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410469331.XA Pending CN104255445A (en) 2014-09-15 2014-09-15 Selective breeding method of new spring-sowing ecological type vegetable soybean variety

Country Status (1)

Country Link
CN (1) CN104255445A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105941135A (en) * 2016-06-01 2016-09-21 安志强 Cultivating technology of green soy beans
CN106900546A (en) * 2017-03-08 2017-06-30 上海市农业科学院 It is a kind of to keep the stable seed growing method of environmental soybean varieties kind of sowing in spring
CN106962172A (en) * 2017-02-14 2017-07-21 南京市蔬菜科学研究所 A kind of breeding method of Cowpea Variety kind
CN110050686A (en) * 2019-04-09 2019-07-26 武汉弘耕种业有限公司 A kind of selection of disease-resistant high-quality food new soybean varieties
CN114303851A (en) * 2021-12-23 2022-04-12 贵州省油料研究所(贵州省香料研究所) High-yield shade-tolerant soybean variety breeding method suitable for intercropping

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1252211A (en) * 1998-10-22 2000-05-10 王明炎 Vegetable soya bean cultivating process
CN1944649A (en) * 2006-10-27 2007-04-11 上海市农业科学院 Vegetable soy bean DNA finger print map and its construction method and use
CN102246647A (en) * 2011-05-28 2011-11-23 上海市农业科学院 Breeding and cultivation method of spring sowing type vegetable soybean
CN102845298A (en) * 2012-09-20 2013-01-02 江苏省农业科学院 Method for culturing large-grain soybean variety for summer sowing vegetables

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1252211A (en) * 1998-10-22 2000-05-10 王明炎 Vegetable soya bean cultivating process
CN1944649A (en) * 2006-10-27 2007-04-11 上海市农业科学院 Vegetable soy bean DNA finger print map and its construction method and use
CN102246647A (en) * 2011-05-28 2011-11-23 上海市农业科学院 Breeding and cultivation method of spring sowing type vegetable soybean
CN102845298A (en) * 2012-09-20 2013-01-02 江苏省农业科学院 Method for culturing large-grain soybean variety for summer sowing vegetables

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MAKIKO MIMURA ET AL.: "SSR diversity of vegetable soybean [Glycine max (L.) Merr.]", 《GENETIC RESOURCES AND CROP EVOLUTION》 *
杨加银: "菜用大豆育种目标与策略的商榷", 《种子》 *
陈远东等: "菜用大豆耐低温弱光鉴定方法和评价指标的研究", 《中国农学通报》 *
顾卫红等: "菜用大豆新品种"青酥二号"的选育", 《上海农业学报》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105941135A (en) * 2016-06-01 2016-09-21 安志强 Cultivating technology of green soy beans
CN106962172A (en) * 2017-02-14 2017-07-21 南京市蔬菜科学研究所 A kind of breeding method of Cowpea Variety kind
CN106900546A (en) * 2017-03-08 2017-06-30 上海市农业科学院 It is a kind of to keep the stable seed growing method of environmental soybean varieties kind of sowing in spring
CN110050686A (en) * 2019-04-09 2019-07-26 武汉弘耕种业有限公司 A kind of selection of disease-resistant high-quality food new soybean varieties
CN114303851A (en) * 2021-12-23 2022-04-12 贵州省油料研究所(贵州省香料研究所) High-yield shade-tolerant soybean variety breeding method suitable for intercropping

Similar Documents

Publication Publication Date Title
CN102986425A (en) Tea tree and soybean intercropping pattern
CN104255445A (en) Selective breeding method of new spring-sowing ecological type vegetable soybean variety
CN110150136A (en) A kind of breeding method of resistance to storage and transportation fine work steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste
Yasoda et al. Effect of Different Shade Levels on Growth and Yield Performances of Cauliflower.
CN104823832A (en) Disease resistant high quality vegetable soybean new kind seed selection method
CN104221675B (en) Fresh waxy corn interplanting early Chinese cabbage multiple cropping autumn kidney bean 1 year two season three ripe efficient cultivation method
CN108782091A (en) Method for improving seed reproduction yield of asparagus bean in south China
CN104604671A (en) Breeding method for novel vegetable soybean variety with wide adaptability
CN102835306A (en) Breeding method for early-maturing hybrid broccoli in additive selection
JP2003052239A (en) Method for cultivating pachyrhizus erosus
CN104160855A (en) Method for improving quality and increasing number of collard seed production
Singh et al. Jamun
CN106900546B (en) It is a kind of to keep the stable seed growing method of environmental soybean varieties kind of sowing in spring
CN114097600B (en) Breeding method of high-yield and strong-disease-resistance round cabbage
CN107318470A (en) A kind of method for cultivating apocarya fast-growing breeding stock
Hossain et al. Effects of water stress on yield attributes and yield of different mungbean genotypes
CN104255438B (en) A kind of selection of forage grass type Semen sojae atricolor
CN102715080A (en) Cold resistance screening method for northern latitude 37-degree winter sowed pea resources
CN105875151A (en) Rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance
Pandey et al. Evaluation of pole-type French bean genotypes in the mid hills of western Nepal.
JP6277911B2 (en) How to seed Koganebana
Abdelmageed et al. Performance of different tomato genotypes in the arid tropics of Sudan during the summer season. I. Vegetative growth
KR102342057B1 (en) Cold Stress and Clubroot Disease Resistant Novel Chinese Cabbage Variety
Rahman Factors limiting the expansion of summer pulses
Kong et al. Effects of Within-row Intercropping with Snow Peas on Yield and Quality of Cherry Tomatoes in an Organic High Tunnel Production System

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20150107