CN105875151A - Rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance - Google Patents
Rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance Download PDFInfo
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- CN105875151A CN105875151A CN201610247543.2A CN201610247543A CN105875151A CN 105875151 A CN105875151 A CN 105875151A CN 201610247543 A CN201610247543 A CN 201610247543A CN 105875151 A CN105875151 A CN 105875151A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance. The method comprises the steps of selecting Brassic campestris ssp. chinensis Makino seeds which are full and uniform in size, sowing the seeds in a nutrient pot containing wet garden soil, spreading a layer of fine garden soil on the surface of the seeds after the seeds are sowed, covering the soil with a sunshade net to preserve moisture for cultivation, and taking off the sunshade net after sprouts of the seeds come out of the soil; when cotyledons of seedlings of Brassic campestris ssp. chinensis Makino are fully unfolded, screening the seedlings once, putting the seedlings in a biochemical tank, filling water until the surface of the water fully submerges the seedlings, putting the biochemical tank under a 32 to 35 DEG C environment, taking out the seedlings from the biochemical tank after two days, performing water-controlled cultivation to enable the seedlings to restore to grow, and statistically collecting the survival rate of the seedlings five days after restoration to growth for identifying the waterlogging tolerance of Brassic campestris ssp. chinensis. By using the rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance, when the cotyledons of the seedlings of Brassic campestris ssp. chinensis Makino are fully unfolded, waterlogging-tolerant or waterlogging-sensitive Brassic campestris ssp. chinensis Makino is identified by using a fully water flooding method and investigating the survival situation of plants.
Description
Technical field
The invention belongs to crop in cruciferae resistance identification technology field, be specifically related to one not balling white
The rapid identification method of dish moisture-proof.
Background technology
Wet damage or crop failure caused by waterlogging is the major natural disasters of many countries in the world.Report according to FAO (Food and Agriculture Organization of the United Nation) (FAO)
Accusing and world's pedological map estimation of International Society of Soil Science's drafting, the most runny soil accounts for 12%,Its
The arable land in the middle whole world about 10% suffers wet damage caused by waterlogging evil, and the irrigated land of about 50% is affected by impeded drainage, leads
Cause crop failure about 20%。China be a waterlogging disaster than more serious country, and gesture in rise year by year,
Especially in the great rivers basin such as the Changjiang river, the Yellow River, the impetus that ecological environment runs down does not obtains essence
Change, flood occurs now and then, and brings harm to the growth of plant, brings to the productive life of people
Huge loss.
Chinese cabbage (Brassic campestris ssp.chinensis Makino) has another name called Plantula Brassicae chinensis,
Green vegetable, originate in China, belong to Cruciferae Vegetables in Brassica.Chinese cabbage is tender with quality, unique flavor,
The feature such as nutritious, occupies an important position in people's lives, particularly the middle and lower reach of Yangtze River and on the south
Area, is popular vegetable that people must eat every day, indispensable.Simultaneously as it is wide in variety, plant
Planting area big, it is possible to the whole year carries out High-quality Cultivation, year-round supply, in lower Yangtze area, not balling is white
The area that produces of dish accounts for the 30～40% of vegetable multiple cropping area.But the lower Yangtze area of China is that China is wet
One of area that damage or crop failure caused by waterlogging is the most serious, especially summer and autumn meet with the impact of Heavy Rain of Typhoon often, during winter in spring
Having a run of wet weather, wet damage or crop failure caused by waterlogging to have a strong impact on its growth, cause its underproduction, quality reduces, and even causes and ruins product.
At present both at home and abroad the most to the research of Cruciferae moisture-proof, but be concentrated mainly on Brassica campestris L, Brassica campestris L. ssp.chinensis and
Chinese cabbages etc., for the moisture-proof research of Brassica campestris L, Brassica campestris L. ssp.chinensis and Chinese cabbage it has been reported that resistance to Chinese cabbage
Though moist authentication method also has been reported that, wait by the Chinese cabbage of different moisture-proofs is studied as,
Finally determine, relative air humidity 95%, 30 DEG C/20 DEG C (daytime 12h/ night 12h) place saturated with soil moisture
Manage 6 leaf phase seedling age 6 days, as the qualification condition of Chinese cabbage moisture-proof difference, with blade chlorisis area
With degree as wet injury graded index, investigate Wet-damage index with this, thus identify Chinese cabbage moisture-proof,
But the Chinese cabbage seedling age selected by the method is too big, identify that flooding degree is the lowest, identify that temperature is on the low side,
Identification of indicator is the most complicated, applies loaded down with trivial details, the moisture-proof Rapid identification of inapplicable high-volume resource, is not suitable for
Popularization and application.
Therefore, it is badly in need of wanting an authentication method that can identify Chinese cabbage moisture-proof fast and accurately, for
The screening of Chinese cabbage moisture-proof germ plasm resource provides technical support.
Summary of the invention
It is an object of the invention to provide the rapid identification method of a kind of Chinese cabbage moisture-proof, it is intended to solve
The Chinese cabbage seedling age selected the authentication method of Chinese cabbage moisture-proof the most at present is too big, identifies waterflooding
Degree is the lowest, identifies that temperature is on the low side, and identification of indicator is the most complicated, applies loaded down with trivial details, inapplicable high-volume resource
The problem of moisture-proof Rapid identification.
The present invention is achieved in that the rapid identification method of a kind of Chinese cabbage moisture-proof, and this is not tied
The rapid identification method of ball Chinese cabbage moisture-proof comprises the following steps:
Choose Chinese cabbage seed, be sown in nutritive cube, carry out seed germination；
After the seedling of Chinese cabbage grows, seedling is screened；
Seedling will be filtered out be placed in biochemical case, and be filled to the water surface and flood filter out seedling completely, be placed in
Water-saving irrigntion is carried out under 32-35 DEG C of environment；
Seedling after water-saving irrigntion is taken out from biochemical case, carries out controlling the seedling after water makes water-saving irrigntion and recover
Naturally growth, recovers the survival rate of the seedling after adding up water-saving irrigntion after nature grows a period of time, and carries out
The qualification of the moisture-proof of Chinese cabbage.
Further, choosing Chinese cabbage seed method is: choose full seed, not balling of uniform size
Chinese cabbage seed；Described being sown in nutritive cube, carrying out seed germination is: choose full seed, size uniform
Chinese cabbage seed uniform broadcasting in the nutritive cube equipped with moistening garden mould, after seed broadcasting, at seed
One layer of fine garden mould is spread on surface, covers water-retaining cultivation with shade net, takes down shade net after seed shows money or valuables one carries unintentionally,
Carry out seed germination.
Further, after the seedling of Chinese cabbage grows, seedling is carried out screening technique is: when balling is not white
When the seedling cotyledon of dish is fully deployed, seedling is carried out primary screening, pull out the seedling that growing way is small and weak, retain
The seedling that growing way is good and consistent；
Further, seedling will be filtered out and be placed in biochemical case, and be filled to the water surface and flood filter out seedling completely,
Carrying out water-saving irrigntion method is: be placed on by the seedling that the growing way filtered out is good and consistent in biochemical case, water filling
Flood the seedling that the growing way filtered out is good and consistent to the water surface completely, and keep two angel's waters surface to flood sieve always
The growing way selected is good and consistent seedling, carries out water-saving irrigntion.
Further, recover to add up after nature grows five days the survival rate of aforementioned seedling, carry out Chinese cabbage
The qualification of moisture-proof.
Further, the screening technique of seed kernel is: after seed harvest, is first sieved by the impurity sieves such as kind of pod
Remove, then with special seed purifier, seed is finely screened, to screen out impurity, the fullest, not rounding
Seed, carry out the most selected to determine that Chinese cabbage identifies seed used the most again.
Further, the breeding method of Chinese cabbage is: select not cultivate the clean garden mould of Chinese cabbage,
Cultivate the double color nutritious alms bowl of plastics that container selection model is medium, moistening garden mould is loaded at the 3/4 of nutritive cube, treats
Spread the moistening garden mould of a layer fine the most again at the surface of the seed, seed is completely covered, and adds a cover shade net
Moisturizing.
Further, the period that Chinese cabbage moisture-proof rapidity is identified is: when Chinese cabbage, to grow to cotyledon complete
When entirely launching.
Further, during water-saving irrigntion and identify that environmental condition is: keep water during whole water-saving irrigntion
Seedling cotyledon is flooded in face always；High for summer in month at 7-8 for keeping variations in temperature to stablize whole qualification environmental condition
Glasshouse under the conditions of temperature is carried out.
The present invention is by carrying out full submerging treatment to Chinese cabbage cotyledon seedling time fully deployed and plant goes
After water logging restoration ecosystem, resistance to survival rate of plant height the state that can restore normal growth after slow Seedling of flooding, and not
The resistance to plant flooded is all dead or death rate high or anhydrate restoration ecosystem time dead or anhydrate restoration ecosystem time delay Seedling
Slowly, thus the kind matter of moisture-proof and not moisture-proof is made a distinction, reach to obtain moisture-proof kind and germplasm materials
Purpose；The Chinese cabbage seedling that the present invention selects is cotyledon period, and seedling age is little, and uses at full waterflooding
Reason, and the glasshouse under the conditions of the natural high-temperature in 7-8 month in summer is carried out, sowing container the most easily selects,
Qualification condition is simple and convenient, can carry out large batch of authentication process, it is easy to promote；The present invention does not only differentiates between
Chinese cabbage kind and the germ plasm resource moisture-proof ability to wet damage or crop failure caused by waterlogging, and can be carried by this authentication method
The moisture-proof of high a certain Chinese cabbage material colony differentiates speed, for Chinese cabbage moisture-proof new varieties
Selection-breeding provide more moisture resistant material, for Chinese cabbage breeding for stress tolerance practice and theoretical research on all
The most valuable.
Accompanying drawing explanation
Fig. 1 is the rapid identification method flow chart of the Chinese cabbage moisture-proof that the embodiment of the present invention provides.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment,
The present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to
Explain the present invention, be not intended to limit the present invention.
Below in conjunction with the accompanying drawings the application principle of the present invention is further described.
As shown in Figure 1: the rapid identification method of the Chinese cabbage moisture-proof of the embodiment of the present invention include with
Lower step:
S101: choose Chinese cabbage seed, is sown in nutritive cube, carries out seed germination；
After the seedling of S102: Chinese cabbage grows, seedling is screened；
S103: will filter out seedling and be placed in biochemical case, is filled to the water surface and floods filter out seedling completely,
It is placed in carrying out water-saving irrigntion under 32-35 DEG C of environmental condition；
S104: take out the seedling after water-saving irrigntion from biochemical case, carries out controlling the children after water makes water-saving irrigntion
Seedling recovers nature growth, recovers the survival rate of the seedling after adding up water-saving irrigntion after nature grows a period of time,
And carry out the qualification of the moisture-proof of Chinese cabbage.
Choosing Chinese cabbage seed is: choose full seed, Chinese cabbage seed of uniform size；Institute
Stating and be sown in nutritive cube, carrying out seed germination is: choose full seed, Chinese cabbage of uniform size
Seed uniform broadcasting is in the nutritive cube equipped with moistening garden mould, after seed broadcasting, spreads one layer thin at the surface of the seed
Thin garden mould, covers water-retaining cultivation with shade net, takes down shade net after seed shows money or valuables one carries unintentionally, and carries out seed germination.
After the seedling of Chinese cabbage grows, seedling is carried out screening is: when the seedling cotyledon of Chinese cabbage
Time fully deployed, seedling is carried out primary screening, pull out the seedling that growing way is small and weak, retain growing way good and consistent
Seedling；
Seedling will be filtered out be placed in biochemical case, and be filled to the water surface and flood filter out seedling completely, flood
Aquaponic is: is placed on by the seedling that the growing way filtered out is good and consistent in biochemical case, is filled to the water surface complete
Flood the seedling that the growing way filtered out is good and consistent, and keep two angel's waters surface to flood the growing way filtered out always
Good and consistent seedling, carries out water-saving irrigntion.
Seedling after water-saving irrigntion is taken out from biochemical case and is: water-saving irrigntion is two days later by after water-saving irrigntion
Seedling is taken out from biochemical case；The seedling after water-saving irrigntion is added up in described recovery after naturally growing a period of time
Survival rate carries out being accredited as of the moisture-proof of Chinese cabbage: recover to add up aforementioned seedling after nature grows five days
Survival rate carry out the qualification of moisture-proof of Chinese cabbage.
The screening technique of seed kernel is: after seed harvest, is first screened out by the impurity sieves such as kind of pod, then uses
Seed is finely screened by special seed purifier, to screen out impurity, seed the fullest, not rounding,
Finally carry out the most selected to determine that Chinese cabbage identifies seed used the most again.
The type of rearing of Chinese cabbage is: selects not cultivate the clean garden mould of Chinese cabbage, cultivates and hold
The double color nutritious alms bowl of plastics that model is medium selected by device, is loaded at the 3/4 of nutritive cube by moistening garden mould, treats after planting
Spread the moistening garden mould of a layer fine again at the surface of the seed, seed is completely covered, and adds a cover shade net moisturizing.
The period that Chinese cabbage moisture-proof rapidity is identified is: when Chinese cabbage grow to cotyledon fully deployed time.
During water-saving irrigntion and identify that environmental condition is: keep the water surface to flood during whole water-saving irrigntion always
No seedling cotyledon；For keeping variations in temperature to stablize whole qualification environmental condition under the conditions of 7-8 month summer high temperature
The glasshouse of (32-35 DEG C) is carried out.
Below in conjunction with specific embodiment, the application principle of the present invention is further described.
Embodiment 1, the moisture-proof of the Chinese cabbage of different cultivars is identified
Material to be tested: all material in embodiment is by Shanghai academy of agricultural sciences horticulture institute green vegetable problem
Group provides.Selected kind be the middle and lower reach of Yangtze River and on the south the current main breed in region, and pass through
Cultivation practices for many years has had understanding to the moisture-proof of these kinds.No. 1 is that new short green grass or young crops is (medium
Moisture-proof), No. 2 be the new summer blue or green No. 2 (not moisture-prooves), No. 3 be SUZHOUQING(sic) (not moisture-proof), No. 4 for black
Oil winter (moisture-proof).
Test method: test is carried out in greenhouse, Inst. of Gardening, Academy of Agriculture of Shanghai proving ground.
Choose full seed, the Chinese cabbage seed of four kinds of different cultivars of uniform size, uniform broadcasting equipped with
In the nutritive cube of moistening garden mould, after seed broadcasting, spread one layer of fine garden mould at the surface of the seed, use shade net
Covering water-retaining cultivation, for the accuracy of guarantee test, each kind is respectively provided with 3 repetitions, and arranges not
Submerging treatment is comparison (CK)；When the seedling cotyledon of the Chinese cabbage of experimental cultivar is fully deployed, to children
Seedling carries out primary screening, pulls out the seedling that growing way is small and weak, retains the seedling that growing way is good and consistent；Will be for examination children
Seedling is placed in biochemical case, and each kind is all provided with 3 times and repeats, and repeats for three times to be placed on different biochemical case
In, it is filled to the water surface and floods completely for examination seedling, and keep the water surface in qualification process to flood for examination seedling always,
Cultivate one day, two days and three days with this understanding；Respectively will behind full water-saving irrigntion one day, two days and three days
Taking out from biochemical case for examination seedling, control hydroponics makes to recover nature growth for examination seedling, recovers nature growth
After five days, statistics is used for identifying the moisture-proof of Chinese cabbage for the survival rate of examination seedling.Seedling percent=(flood
Water survives plant number/experimental cultivar total plant number after recovering) × 100%.Different cultivars Chinese cabbage moisture-proof
Qualification result (seeing table 1).
The qualification result of table 1 different cultivars Chinese cabbage moisture-proof
As shown in Table 1, the Chinese cabbage of different cultivars, at different Submergence time, recovers raw after taking-up
Long seedling percent significant difference.After one day restoration ecosystem of waterflooding, the seedling of four different cultivars is the most slow
All survive after Seedling；The survival rate of the seedling after three days restoration ecosystem of waterflooding is the most relatively low, it is impossible to by difference
The Variety identification of moisture-proof degree comes；And the seedling percent of waterflooding restoration ecosystem two days later shows as 4
Number ＞ No. 2 ＞ of No. 1 ＞ 3, consistent with the result of cultivation practices gained of the present invention.Each not balling is white
Vegetable kind, because of different Screening of Germplasm selection-breeding gained, indirectly obtains the material of different germ plasm resource
Moisture-proof is different, can not only obtain different cultivars Chinese cabbage moisture-proof by this kind of authentication method
Qualification result, the moisture-proof degree of Chinese cabbage difference Germplasms can be identified equally, for
The new varieties of selection-breeding Chinese cabbage moisture-proof provide foundation.For the material of medium moisture-proof, Ke Yigen
According to the different moisture-proof abilities resistance to wet stock of separation screening the most targetedly, resistance to for Chinese cabbage
The selection-breeding of moist new varieties provides more germ plasm resource.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Any amendment, equivalent and the improvement etc. made within bright spirit and principle, should be included in the present invention
Protection domain within.
Claims (8)
1. the rapid identification method of a Chinese cabbage moisture-proof, it is characterised in that this not balling white
The rapid identification method of dish moisture-proof comprises the following steps:
Choose Chinese cabbage seed, be sown in nutritive cube, carry out seed germination；
After the seedling of Chinese cabbage grows, seedling is screened；
Seedling will be filtered out be placed in biochemical case, and be filled to the water surface and flood the seedling filtered out completely, at 32 DEG C
Water-saving irrigntion is carried out under-35 DEG C of environment；
Seedling after water-saving irrigntion is taken out from biochemical case, carries out controlling water, make the seedling after water-saving irrigntion extensive
Multiple nature growth, adds up the survival rate of the seedling after water-saving irrigntion, and carries out not balling after recovering nature growth
The qualification of the moisture-proof of Chinese cabbage.
2. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 1, it is characterised in that
Choosing Chinese cabbage seed method is:
After seed harvest, first kind of a pod sieve is screened out, then with seed purifier, seed is finely screened,
To screen out containing impurity, seed the fullest, not rounding, carry out the most selected the most again
Chinese cabbage identifies seed used, selects full seed, Chinese cabbage seed of uniform size.
3. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 1, it is characterised in that
Being sown in nutritive cube, carrying out seed germination method is:
Choose full seed, Chinese cabbage seed uniform broadcasting of uniform size in the battalion equipped with moistening garden mould
Support in alms bowl, after seed broadcasting, spread one layer of fine garden mould at the surface of the seed, cover water-retaining cultivation with shade net,
After seed shows money or valuables one carries unintentionally, take down shade net, carry out seed germination.
4. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 1, it is characterised in that
After the seedling of Chinese cabbage grows, seedling is carried out screening technique is:
When the seedling cotyledon of Chinese cabbage is fully deployed, seedling is carried out primary screening, pulls out growing way weak
Little seedling, retains the seedling that growing way is good and consistent.
5. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 1, it is characterised in that
Seedling will be filtered out be placed in biochemical case, and be filled to the water surface and flood filter out seedling completely, carry out waterflooding training
Breeding method is:
The seedling that the growing way filtered out is good and consistent is placed in biochemical case, is filled to the water surface and floods sieve completely
The seedling that the growing way selected is good and consistent, under 32 DEG C of-35 DEG C of environment, and keeps two angel's waters surface to flood always
The growing way filtered out is good and consistent seedling, carries out water-saving irrigntion.
6. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 1, it is characterised in that
Recover the survival rate of the seedling after adding up water-saving irrigntion after nature grows five days, carry out the moisture-proof of Chinese cabbage
The qualification of property.
7. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 3, it is characterised in that
The breeding method of Chinese cabbage seed is:
Selecting not cultivate the clean garden mould of Chinese cabbage, the plastics cultivating container selection model medium are double-colored
Nutritive cube, loads moistening garden mould at the 3/4 of nutritive cube, treats to spread one layer fine at the surface of the seed the most again
Moistening garden mould, is completely covered seed, and adds a cover shade net moisturizing.
8. the rapid identification method of Chinese cabbage moisture-proof as claimed in claim 1, it is characterised in that
The qualification of the moisture-proof of described Chinese cabbage is carried out in 7-8 month and in glasshouse.
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| CN201610247543.2A CN105875151A (en) | 2016-04-20 | 2016-04-20 | Rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance |
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| CN201610247543.2A CN105875151A (en) | 2016-04-20 | 2016-04-20 | Rapid identification method of Brassic campestris ssp. chinensis Makino waterlogging tolerance |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107543904A (en) * | 2017-09-28 | 2018-01-05 | 江苏丘陵地区镇江农业科学研究所 | A kind of method for evaluating rape water-logging resistance |
| CN112430601A (en) * | 2020-11-23 | 2021-03-02 | 安徽农业大学 | Cold resistance related gene of non-heading Chinese cabbage and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4109026A (en) * | 1977-07-25 | 1978-08-22 | The United States Of America As Represented By The Secretary Of The Army | Process for producing compacted, dehydrated, uncooked cabbage and cabbage product thereof |
| CN101315358A (en) * | 2008-07-17 | 2008-12-03 | 江汉大学 | Technical appraisement method for genetype moisture-proof ability of cowpea |
| CN101990821A (en) * | 2010-10-14 | 2011-03-30 | 中国农业科学院油料作物研究所 | Method for identifying moisture resistance of sesame during germination |
| CN104404124A (en) * | 2014-11-20 | 2015-03-11 | 上海市农业科学院 | Identification method for clubroot resistance of non-heading cabbages |
-
2016
- 2016-04-20 CN CN201610247543.2A patent/CN105875151A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4109026A (en) * | 1977-07-25 | 1978-08-22 | The United States Of America As Represented By The Secretary Of The Army | Process for producing compacted, dehydrated, uncooked cabbage and cabbage product thereof |
| CN101315358A (en) * | 2008-07-17 | 2008-12-03 | 江汉大学 | Technical appraisement method for genetype moisture-proof ability of cowpea |
| CN101990821A (en) * | 2010-10-14 | 2011-03-30 | 中国农业科学院油料作物研究所 | Method for identifying moisture resistance of sesame during germination |
| CN104404124A (en) * | 2014-11-20 | 2015-03-11 | 上海市农业科学院 | Identification method for clubroot resistance of non-heading cabbages |
Non-Patent Citations (2)
| Title |
|---|
| 张永吉: "不结球白菜耐湿性鉴定及其生理机制研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 李云等: "油菜苗期耐淹性快速筛选方法的建立及验证", 《中国油料作物学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107543904A (en) * | 2017-09-28 | 2018-01-05 | 江苏丘陵地区镇江农业科学研究所 | A kind of method for evaluating rape water-logging resistance |
| CN112430601A (en) * | 2020-11-23 | 2021-03-02 | 安徽农业大学 | Cold resistance related gene of non-heading Chinese cabbage and application thereof |
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Application publication date: 20160824 |
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| RJ01 | Rejection of invention patent application after publication |