CN110150136A - A kind of breeding method of resistance to storage and transportation fine work steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste - Google Patents
A kind of breeding method of resistance to storage and transportation fine work steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste Download PDFInfo
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Abstract
The present invention relates to a kind of breeding methods of the fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste, comprising the following steps: female parent, through 7 generation Single-plant selections, carries out mixing and reserve seed for planting using spring snow Fructus Lycopersici esculenti as source material；Male parent is using Di Fenni tomato as source material, using continuous selfing and molecular marker-assisted selection method, through 4 generation Single-plant selections, 4 generation strains are obtained, in conjunction with resisting etiolation curve leaf disease virus, using continuous selfing and molecular marker-assisted selection method, through 2 generation Single-plant selections, 6 generation strains are obtained, most afterwards through 2 generation Single-plant selections, hybrid strain is removed, mixing is carried out and reserves seed for planting；Maternal and male parent is cross-breeding, the seed of the fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste is obtained.The features such as tomato that the present invention selects has fruit in good taste, and pol is high, and fruit type is straight, and color is good, resistance to storage and transportation, and plant strain growth is healthy and strong, comprehensive disease resistance is strong.
Description
Technical field
The present invention relates to tomato breeding and technical field of cultivation, in particular to a kind of fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste
Breeding method.
Background technique
Tomato, i.e. tomato (scientific name: Lycopersicon esculentum Mill.), are one kind of Solanaceae tomato genus
Annual or herbaceos perennial originates from South America.Tomato is that one kind had not only belonged to vegetables but also belonged to the food of fruit, nutrition
Value is high, rich in vitamin, dietary fiber, minerals, organic acid and polyphenoils etc..Tomato has wide in variety, yield height, battalion
Support it is abundant, it is widely used the advantages that.Other foods in addition, tomato is either arranged in pairs or groups, still stew soup, quick-fried etc. suffer from render palatable,
The effect of fresh, polishing is mentioned, therefore very popular, demand is constantly increasing, and cultivated area is also being growing.
In China, tomato breeding is more external to start late, but the method for breed breeding recent two decades have it is considerable into
Step.With the development of tomato facility cultivation, the demand of storage tolerance, Tomato Quality is declined.The influence of continuous cropping obstacle simultaneously, disease
Insect pest is increasingly severe, such as tomato yellow leaf curl virus sick (TY), root-knot nematode pest and disease damage have seriously affected tomato production.It adopts
With modern breeding method, it is very necessary that the new varieties of the cultivation fine work of resistance to storage and transportation tomato in good taste meet market needs.
Summary of the invention
The present invention for the technical problems in the prior art, provides one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste
Breeding method, the tomato selected have fruit in good taste, and pol is high, and fruit type is straight, and color is good, resistance to storage and transportation, and plant strain growth is strong
Strong, the features such as comprehensive disease resistance is strong.
The technical scheme to solve the above technical problems is that a kind of fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste educates
Kind method, comprising the following steps:
1) maternal breeding: using spring snow Fructus Lycopersici esculenti as source material, with anti-root-knot nematode (Mi), fruit is in good taste, deep powder
Red, robust growth ability of continuously bearing fruit is breeding objective by force, using continuous selfing and molecular marker-assisted selection method, through 7
It for Single-plant selection, carries out mixing and reserves seed for planting, obtain high for self-mating system female parent；
2) breeding of male parent: using Di Fenni tomato as source material, with the resistance to storage and transportation of fruit, fruit type is good, plant strain growth is healthy and strong,
Anti- tomato yellow leaf curl virus is breeding objective, using continuous selfing and molecular marker-assisted selection method, is selected through 4 generation single plants
It selects, obtains 4 generation strains, in conjunction with resisting etiolation curve leaf disease virus, using continuous selfing and molecular marker-assisted selection method, through 2 generations
Single-plant selection obtains 6 generation strains, most removes hybrid strain through 2 generation Single-plant selections afterwards, carries out mixing and reserves seed for planting, and obtains height for self-mating system father
This；
3) crossbreeding: maternal and male parent is cross-breeding, the kind of the fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste is obtained
Son；
Breeding plants breeding using early spring cold canopy and the cold canopy of summer sowing in the step 1) and step 2).
Based on the above technical solution, the present invention can also be improved as follows.
Further, the cold canopy of the summer sowing plants breeding, comprising the following steps:
1. sowing: in mid or late June, the seed that vernalization is showed money or valuables one carries unintentionally matrix hole plate seedling growth；
2. field planting and field management: mid or late July, field planting, built was cultivated after seedling length to 4~5 true leaves, and single pole is whole
Branch, every plant is stayed 4~6 tongue fur fruits, and 2 leaf toppings are stayed at the top of last Honoka sequence；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4, from the second generation point
From group carry out Single-plant selection, the method selected is Marker-assisted selection, then in fructescence according to the appearance of fruit
Quality and nutritional quality preferentially carry out Single-plant selection；
4. selfed seed harvests: to Fruit Ripening of Tomato, fruit being taken to remove the peel, mash, ferment, after cleaning filtering, sunning, obtained
Selfed seed.
Further, the cold canopy of the summer sowing plants breeding, comprising the following steps:
1. sowing: in mid or late June, the seed that vernalization is showed money or valuables one carries unintentionally matrix hole plate seedling growth；
2. field planting and field management: mid or late July, field planting, built was cultivated after seedling length to 4~5 true leaves, and single pole is whole
Branch, every plant is stayed 4~6 tongue fur fruits, and 2 leaf toppings are stayed at the top of last Honoka sequence；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4, from the second generation point
From group carry out Single-plant selection, the method selected is Marker-assisted selection, then in fructescence according to the appearance of fruit
Quality and nutritional quality preferentially carry out Single-plant selection；
4. selfed seed harvests: to Fruit Ripening of Tomato, fruit being taken to remove the peel, mash, ferment, after cleaning filtering, sunning, obtained
Selfed seed.
Further, in the sowing step, vernalization show money or valuables one carries unintentionally seed the preparation method comprises the following steps: tomato seeds are placed in 50~55 DEG C
Water in stir, drop to 25~35 DEG C of stoppings stirring to water temperature, soak seed 5~8h, and then vernalization obtains at 28~30 DEG C.
Further, in the sowing step, the method for nursery are as follows: the every cave of hole tray is put into the seed that 1 vernalization shows money or valuables one carries unintentionally, and will broadcast
Hole tray after kind is placed on vinyl house or the indoor seedbed of sunlight, and 25 DEG C of daytime temperature or more, 16~20 DEG C of night temperature are kept in canopy；
After emergence, maintain 20~25 DEG C of daytime, 10~15 DEG C of night.
Further, in the method for the nursery, further include having when tomato seedling has excessive growth trend, spray the multiple-effect of 20mg/L
The step of azoles solution is controlled.
Further, in the field planting and field management step, the method for field planting are as follows: be colonized in furrow face duplicate rows, furrow are wide
1.25m, seeding row spacing 0.4m × 0.55m, 2200 plants/acre of plantation density.
Further, in the selfing Single-plant selection step, the method for artificial supplementary pollination is using just opening in same strain
Pollen is taken on the anther for the flower put, or is derived from the pollen that oneself takes, carries out supple-mentary pollination, and every fringe stays fruit 3~4.
Further, the step 3) crossbreeding, comprising the following steps:
1. space isolation and sowing: Parent ratio presses paternal plant: planting maternal plant=1:4~8 kind；
2. field management: florescence removal maternal plant pollinates and open flower not in time；
3. pollination: selecting to open and flower that petal is not switched on peels off anther cylinder emasculation on female parent, take in male parent
Pollen is awarded on the gynoecium of maternal emasculation flower, and every fringe sits 3~4 fruits；
4. cenospecies harvests: to Fruit Ripening of Tomato, taking fruit remove the peel, mash, fermenting, clean filtering, dry, obtain miscellaneous
Hand over kind.
Further, the pollination step the following steps are included:
1. adopting powder: after dew is dry on the day before pollination, robust growth fruit type beauty is selected from paternal plant and without disease pest
Harmful plant, the picking same day is in full bloom, normotrophic flower, takes out anther and dries in the shade, temperature is no more than 35 DEG C；
2. powder processed: when 8~9 one-tenth of anther dry, take pollen with pollen sieve sieve, and collect pollen set it is standby in a cool and dry place
With；
3. pollination: selecting that petal emasculation will be opened on healthy and strong maternal plant, dip 2. pollen that step is collected with writing brush
It is applied on flower column cap, every fringe sits 3~4 fruits, and other flowers are all removed.
The beneficial effects of the present invention are: the present invention is female parent with 7 red generation self-mating systems of spring snow, it is selfed with 8 generations of Di Fenni
System is cross-breeding for male parent, obtains first-filial generation steamed dumpling with pork, mushrooms and bamboo shoots tomato.The Hybrid belongs to big pink fruit type, middle cooked food
Kind, one dimensional infinite wall, plant overgrows, and growth potential is stronger.Two times pinnate compound leaves, leaf color are green.First inflorescence 6~9 section of tight knot position,
3~4 section of inflorescence interval section, average every inflorescence spend number 6~7, and continuous bearing capacity is strong.Pericarp is colourless, and fruit is round, green fruit
Shoulder is medium, and fruit tip circle is flat, fruit shoulder dimple, fruit surface smooth, there is micro- rib, and 230~260 grams of single fruit weight.Fruit color depth powder, Guo Gejun
Even, fine work fruit rate is high, and taste is sour-sweet, succulence, and hardness is good, has both mouthfeel and resistance to storage and transportation.Anti- TMV, anti-tomato yellow leaf curl disease
Viral disease (TY-1 gene loci), in anti-root-knot nematode (Mi gene loci), anti mosaic virus of tomato.Wide adaptability, be suitable for south,
Northern protecting field does the autumn and prolongs cultivation, southern autumn outdoor cropping.Yield is different according to area and planting type, general every
Per mu yield can reach 5000~7000kg.
Specific embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
The breeding method of the fine work of the resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste of one kind designed by the present invention, comprising the following steps:
1) maternal breeding: using spring snow Fructus Lycopersici esculenti as source material, with anti-root-knot nematode (Mi), fruit is in good taste, deep powder
Red, robust growth ability of continuously bearing fruit is breeding objective by force, using continuous selfing and molecular marker-assisted selection method, through 7
It for Single-plant selection, carries out mixing and reserves seed for planting, obtain high for self-mating system female parent；
2) breeding of male parent: using Di Fenni tomato as source material, with the resistance to storage and transportation of fruit, fruit type is good, plant strain growth is healthy and strong,
Anti- tomato yellow leaf curl virus is breeding objective, using continuous selfing and molecular marker-assisted selection method, is selected through 4 generation single plants
It selects, obtains 4 generation strains, in conjunction with resisting etiolation curve leaf disease virus, using continuous selfing and molecular marker-assisted selection method, through 2 generations
Single-plant selection obtains 6 generation strains, most removes hybrid strain through 2 generation Single-plant selections afterwards, carries out mixing and reserves seed for planting, and obtains height for self-mating system father
This；
3) crossbreeding: maternal and male parent is cross-breeding, the kind of the fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste is obtained
Son；
Breeding plants breeding using early spring cold canopy and the cold canopy of summer sowing in the step 1) and step 2).
Specifically, early spring cold canopy plantation breeding uses following steps:
1. seeding and seedling raising: mid-January, the seed that vernalization is showed money or valuables one carries unintentionally matrix hole plate seedling growth；
2. field planting and field management: late Febuary, long to 4~5 true leaves, field planting, built cultivation, single pole to tomato seedling
Training, every plant is stayed 4~6 tongue fur fruits, and 2 leaf toppings are stayed at the top of last Honoka sequence；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4.It is separated from F2 generation
Group carry out Single-plant selection, the method selected be Marker-assisted selection, as TY resistance Single-plant selection be in seedling stage with TY-1 divide
Son label is detected, and is retained and is contained markd single plant, and removal is free of markd single plant.Then again fructescence according to fruit
Real exterior quality and nutritional quality carries out Single-plant selection；
4. selfed seed harvests: to Fruit Ripening of Tomato, fruit being taken to remove the peel, mash, ferment, cleaning filtering is dried, and is obtained certainly
Hand over kind.
Wherein, the seed that vernalization shows money or valuables one carries unintentionally stirs the preparation method comprises the following steps: tomato seeds are placed in 50~55 DEG C of water, to water
Temperature drop is stirred to 25~35 DEG C of stoppings, soak seed 5~8h, then at 28~30 DEG C vernalization to get.
Step 1. mesostroma can preferably turf, perlite mixture, volume ratio 3:1, another plus 0.1% mass nitrogen
Phosphorus potassium balance compound fertilizer.Matrix used can select German import KLASMANN turf.
Step 1. in nursery preferred method are as follows: the every cave of hole tray is put into the seed that 1 vernalization shows money or valuables one carries unintentionally, by hole tray after planting
It is placed on vinyl house or the indoor seedbed of sunlight, 25 DEG C of daytime temperature or more, 16~20 DEG C of night temperature or so is kept in canopy.Emergence
Afterwards, mulch is opened in time, maintains 20~25 DEG C of daytime, 10~15 DEG C of night, will ventilate cooling in time when more than 28 DEG C, prevent small
Seedling excessive growth.3~5 leaf phase in seedling stage prevents night temperature too low, otherwise will affect bud differentiation, generates lopsided bud.If there is excessive growth to become
Gesture, ventilate cooling in time, and the paclobutrazol solution of also sprayable 20mg/L is controlled.In order to cultivate strong sprout, to be fitted before field planting
When reducing temperature and control humidity, the anti-adversity ability of rice shoot is improved.
Step is 2. middle to be colonized preferably in the following ways: it is colonized in furrow face duplicate rows, furrow wide 1.25m, seeding row spacing 0.4m ×
0.55m, generally about 2200 plants/acre of plantation density.
Step 2. middle fertilising, the optimum condition to water are as follows: base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage nitrogenous fertilizer
Cannot be excessive, prevent plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, it is such as molten using 1%~2% potassium dihydrogen phosphate
Liquid foliage-spray.
3. step is selfed in Single-plant selection, the method for artificial supplementary pollination is preferably with the just open flower in same strain
Anther on take out pollen, or be derived from the pollen that oneself takes, carry out supple-mentary pollination, every fringe stays fruit 3~4.From F2 generation separation
Group carries out Single-plant selection, and the method selected is Marker-assisted selection, if TY resistance Single-plant selection is in seedling stage with TY-1 molecule
Label is detected, and is retained and is contained markd single plant, and removal is free of markd single plant.Then again fructescence according to fruit
Exterior quality and nutritional quality carry out Single-plant selection.
Step 4. in selfed seed harvest preferably use following methods: after pollination of blooming, by growth and development in 45~60 days,
Harvesting ripe tomato fruit is removed the peel, mashes, fermenting, cleaning filtering, sunning, obtaining selfed seed.
The cold canopy of the summer sowing plants breeding, comprising the following steps:
1. seeding and seedling raising: in mid or late June, the seed that vernalization is showed money or valuables one carries unintentionally matrix hole plate seedling growth；
2. field planting and field management: mid or late July, field planting, built was cultivated after seedling length to 4~5 true leaves, and single pole is whole
Branch, every plant is stayed 4~6 tongue fur fruits, and 2 leaf toppings are stayed at the top of last Honoka sequence；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4.It is separated from F2 generation
Group carry out Single-plant selection, the method selected be Marker-assisted selection, as TY resistance Single-plant selection be in seedling stage with TY-1 divide
Son label is detected, and is retained and is contained markd single plant, and removal is free of markd single plant.Then again fructescence according to fruit
Real exterior quality and nutritional quality carries out Single-plant selection；
4. selfed seed harvests: to Fruit Ripening of Tomato, fruit being taken to remove the peel, mash, ferment, cleaning filtering is dried, and is obtained certainly
Hand over kind.
Specifically preferred mode of operation is identical as the preferred embodiment that early spring cold canopy plantation breeding uses for each step.
2. field management is preferably in the following ways for step: every 7~10 days or according to practical feelings of falling ill after field planting slow seedling
The pre- preventing disease and pest of condition laxative occurs.Main diseases and insect pests have: tomato chlorisis virosis, canker, trialeurodes vaporariorum etc..
The crossbreeding, preferably includes following steps:
1. space isolation and sowing: sealing is obturaged with the above fly net of 60 mesh before and after production of hybrid seeds greenhouse, and Parent ratio is pressed
Paternal plant: it plants maternal plant=1:4~8 kind；
2. field management: florescence removal maternal plant pollinates and open flower not in time；
3. pollination: selecting to open and flower that petal is not switched on peels off anther cylinder emasculation on female parent, take in male parent
Pollen is awarded on the gynoecium of maternal emasculation flower, and every fringe sits 3~4 fruits；
4. cenospecies harvests: to Fruit Ripening of Tomato, taking fruit remove the peel, mash, fermenting, clean filtering, dry, obtain miscellaneous
Hand over kind.
Step 1. in the production of hybrid seeds preferred fertile soil, the convenient region in water source, the above insect prevention of 60 mesh on all air ports of production of hybrid seeds canopy
Net is obturaged, to prevent insect-transmitted virus sick.
Step is 3. middle to pollinate by the way of artificial pollination.The preferred operating procedure of artificial pollination are as follows:
1. adopting powder: after dew is dry on the day before pollination, robust growth fruit type beauty is selected from paternal plant and without disease pest
Harmful plant, the picking same day is in full bloom, normotrophic flower, takes out anther and dries in the shade, temperature is no more than 35 DEG C；
2. powder processed: when 8~9 one-tenth of anther dry, take pollen with pollen sieve sieve, and collect pollen set it is standby in a cool and dry place
With；
3. pollination: selecting that petal emasculation will be opened on healthy and strong maternal plant, dip 2. pollen that step is collected with writing brush
It is applied on flower column cap, every fringe sits 3~4 fruits, and other flowers are all removed.Cenospecies harvests in step 4) are as follows: blooms
After pollination, by growth and development in 45~60 days, harvesting ripe tomato fruit was removed the peel, is mashed, fermenting, cleans filtering, dries,
Obtain selfed seed.
It is the preferred embodiment of the invention below
Embodiment 1
The breeding method of the present embodiment, comprising the following steps:
1) maternal breeding
It is one dimensional infinite wall steamed dumpling with pork, mushrooms and bamboo shoots tomato that Syngenta Co., Ltd cultivates that spring snow is red, and plant overgrows, robust growth, and synthesis is disease-resistant
Property strong, anti-tomato yellow leaf curl virus (anti-TY), anti-root-knot nematode.Fruit pink, fruit surface smooth, fruit type is slightly flat, there is green fruit
Shoulder, 250~300g of single fruit weight, continuous bearing capacity are strong.Fruit soluble solids content is high, and fiber is few, sour-sweet than moderate, mouth
Feel.
It is red for source material with spring snow, add generation and the cold canopy of summer sowing to plant using 4 years early springs cold canopy, selects height through 7 generations
For self-mating system female parent, the strain plant strain growth is healthy and strong, and anti-TY, anti-root-knot nematode, continuous fruit setting ability is strong, and fruit is in good taste.
Specific Breeding Process is specific as follows:
1, early spring cold canopy additive selection
1. seeding and seedling raising: spring snow Red Indian race is put into 55 DEG C of water firstly, carrying out seed presoaking and germinating before sowing by mid-January
In stir rapidly, when water temperature drops to 30 DEG C, stop stirring, seed soaking 8h obtain vernalization then in 28 DEG C of at a temperature of vernalization and show money or valuables one carries unintentionally
Sponge gourd seed；Matrix hole tray is prepared, by turf, perlite turf by volume: perlite=3:1 is mixed, and adds matrix quality
Millesimal N P and K balance compound fertilizer mixes well, and covers plastic film moisturizing and stands 12 hours, fills 100 hole hole trays；Most
Afterwards, the tomato seeds to show money or valuables one carries unintentionally through vernalization are sowed in seedling culture hole plate matrix, depth of planting 1.2cm, 1, every cave seed is broadcast
Vermiculite earthing is used after kind；Hole tray after planting is placed on the seedbed in seedling cultivation greenhouse, to make to keep the temperature, on seedbed upper berth hot line
Small plastic shed is covered, 25 DEG C of daytime temperature or more, 18 DEG C of night temperature or so are kept in canopy.After emergence, open film in time, maintain daytime 20~
25 DEG C, 10~15 DEG C of night, will ventilate cooling in time when more than 28 DEG C, prevent seedling excessive growth.In seedling stage 3~5 leaf phase, prevent night
Temperature is too low, otherwise will affect bud differentiation, generates lopsided bud.If there is excessive growth trend, ventilate cooling in time, also sprayable
The paclobutrazol solution of 20mg/L is controlled.Temperature and control humidity are suitably reduced in order to cultivate strong sprout, before field planting, improve seedling
The anti-adversity ability of seedling；
2. field planting and field management: late Febuary, site preparation apply fertilizer, do furrow, and furrow are 1.25 meters wide, cover white mulch, and duplicate rows is fixed
It plants, seeding row spacing 0.4m × 0.55m, is colonized 2200 plants or so per acre；Base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage nitrogen
Fertilizer cannot be excessive, prevents plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, such as uses 1%~2% potassium dihydrogen phosphate
Solution foliage-spray.Netted melon only retains trunk, extract whole side shoots, timely bud picking；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4.It is separated from F2 generation
Group carry out Single-plant selection, the method selected be Marker-assisted selection, as TY resistance Single-plant selection be in seedling stage with TY-1 divide
Son label is detected, and is retained and is contained markd single plant, and removal is free of markd single plant.Then again fructescence according to fruit
Real exterior quality and nutritional quality carries out Single-plant selection；
4. selfed seed harvests: after pollination, tamato fruit selects fruit after fruit annesl through growth and development in 45~60 days
Good, the best in quality fruit harvesting of type, removes the peel, mashes, and 25~30 DEG C ferment 12 hours, so benefits from clear water washing and filtering, will
To seed dry in time, carry out Packaging and Labeling.
2, summer sowing sunshade plastic greenhouse cultivation breeding
1. seeding and seedling raising: mid or late June carries out seed presoaking and germinating before sowing, spring snow Red Indian race is put into 55 DEG C of water
Stirring rapidly stops stirring when water temperature drops to 30 DEG C, and the 8h that soaks seed obtains what vernalization showed money or valuables one carries unintentionally then in 28 DEG C of at a temperature of vernalization
Tomato seeds；Matrix hole tray is prepared, by turf, perlite turf by volume: perlite=3:1 is mixed, and adds matrix quality thousand
/ mono- N P and K balance compound fertilizer mixes well, and covers plastic film moisturizing and stands 12 hours, fills 100 hole hole trays；Finally,
The tomato seeds to show money or valuables one carries unintentionally through vernalization are sowed in seedling culture hole plate matrix, depth of planting 1.2cm, 1, every cave seed, after planting
With vermiculite earthing；Hole tray after planting is placed on the seedbed in seedling cultivation greenhouse, to make to cool down, seedling cultivation greenhouse should use sunshade net
And Wet-curtain temperature reducing.Day temperature is maintained at 25~30 DEG C, 20 DEG C of night temperature or so, prevents seedling excessive growth.3~5 leaf phase in seedling stage prevents
Only temperature is excessively high, otherwise will affect bud differentiation, generates lopsided bud.If there is excessive growth trend, ventilate cooling in time, can also spray
The paclobutrazol solution for spilling 20mg/L is controlled.In order to cultivate strong sprout, suitable control watering hardening is wanted before field planting, improves rice shoot
Anti-adversity ability；
2. field planting and field management: mid or late July, site preparation apply fertilizer, do furrow, and furrow are 1.25 meters wide, cover black mulch film, duplicate rows
Field planting, seeding row spacing 0.4m × 0.55m are colonized 2200 plants or so per acre；Base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage
Nitrogenous fertilizer cannot be excessive, prevents plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, such as the biphosphate of sprinkling 1%~2%
Potassium foliage-spray.Netted melon only retains trunk, extract whole side shoots, timely bud picking；
After field planting, laxative is primary within every 7~10 days, the pest and disease damages such as prevention chlorisis virus, canker, trialeurodes vaporariorum；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4.It is separated from F2 generation
Group carry out Single-plant selection, the method selected be Marker-assisted selection, as TY resistance Single-plant selection be in seedling stage with TY-1 divide
Son label is detected, and is retained and is contained markd single plant, and removal is free of markd single plant.Then again fructescence according to fruit
Real exterior quality and nutritional quality carries out Single-plant selection；
4. selfed seed harvests: after pollination, tamato fruit selects fruit after fruit annesl through growth and development in 45~60 days
Good, the best in quality fruit harvesting of type, removes the peel, mashes, and 25~30 DEG C ferment 12 hours, so benefits from clear water washing and filtering, will
To seed dry in time, carry out Packaging and Labeling.
2) breeding of male parent
Enlightening Pfennig, one dimensional infinite wall steamed dumpling with pork, mushrooms and bamboo shoots tomato, plant overgrow, and stalk is sturdy, and leaf color is black green, and growth potential is more prosperous.Anti- kind
Eggplant yellow leaf curl is viral (anti-TY).Fruit is round, fruit surface smooth, and fruit is uniform, continuous result energy, 200~250g of single fruit weight；Fruit
It is real hard, resistance to storage and transportation.
Using Di Fenni as source material, adds generation and the cold canopy of summer sowing to plant using 4 years early springs cold canopy, select height through 8 generations
For self-mating system male parent, the strain robust growth, anti-TY, continuous fruit setting ability is strong, fine work fruit rate is high, and the hardness of fruit is good, resistance to storage and transportation.
Breeding Process is identical as maternal Breeding Process.
3) crossbreeding
It is cross-breeding for self-mating system female parent and height for self-mating system male parent with height, obtains tomato combination, had
Body crossbreeding process is as follows:
1. sowing: selection fertile soil irrigates convenient plot, the production of hybrid seeds in greenhouse, and front and back is sealed 60 mesh or more and prevented
Worm net is obturaged, and prevents insect from entering.The ratio of Parent presses male parent: female parent=1:4 kind is planted, and Parent marks；
2. field management: the same production management of production of hybrid seeds field management；
3. pollination: artificial pollination specifically: gather and process pollen: after dew is dry on the day before pollination, being selected in paternal plant
Robust growth fruit type is beautiful and the plant of no disease and pests harm, and the picking same day is in full bloom, normotrophic flower, takes out anther and dries in the shade, temperature
Degree is no more than 35 DEG C, in order to avoid pollen aging and death.Anther 8-9 at it is dry when, i.e., available pollen sieve sieve takes pollen, collects pollen
It sets spare in a cool and dry place.Pollination: select will to open flower that petal is not switched on healthy and strong maternal plant with tweezers gently
Anther cylinder emasculation is peelled off, gynoecium of damaging is sure not, paternal pollen is dipped with writing brush and is gently applied on the column cap of emasculation female parent flower,
Sulfuric acid paper bag in rear enclosure of pollinating, ties sack, and listing mark.Every fringe sits 3~4 fruits, other flowers are all removed, to protect
Demonstrate,prove the plumpness of seed kernel.
4. the harvest of cenospecies: after pollination, tamato fruit selected after fruit annesl through growth and development in 45~60 days
Good, the best in quality fruit harvesting of fruit type, removes the peel, mashes, and 25~30 DEG C ferment 12 hours, then uses clear water washing and filtering, will
Obtained seed is dried in time, carries out Packaging and Labeling.
Embodiment 2
The breeding method of the present embodiment, comprising the following steps:
1) maternal breeding
It is one dimensional infinite wall steamed dumpling with pork, mushrooms and bamboo shoots tomato that Syngenta Co., Ltd cultivates that spring snow is red, and plant overgrows, robust growth, and synthesis is disease-resistant
Property strong, anti-tomato yellow leaf curl virus (anti-TY), anti-root-knot nematode.Fruit pink, fruit surface smooth, fruit type is slightly flat, there is green fruit
Shoulder, 250~300g of single fruit weight, continuous bearing capacity are strong.Fruit soluble solids content is high, and fiber is few, sour-sweet than moderate, mouth
Feel.
It is red for source material with spring snow, add generation and the cold canopy of summer sowing to plant using 4 years early springs cold canopy, selects height through 7 generations
For self-mating system female parent, the strain plant strain growth is healthy and strong, and anti-TY, anti-root-knot nematode, continuous fruit setting ability is strong, and fruit is in good taste.
Specific Breeding Process is specific as follows:
1, early spring cold canopy additive selection
1. seeding and seedling raising: spring snow Red Indian race is put into 54 DEG C of water firstly, carrying out seed presoaking and germinating before sowing by early January
In stir rapidly, when water temperature drops to 32 DEG C, stop stirring, soak seed 7.5h, then in 27 DEG C of at a temperature of vernalization, obtains vernalization dew
White tomato seeds；Matrix hole tray is prepared, by turf, perlite turf by volume: perlite=3:1 is mixed, and adds matrix matter
It measures millesimal N P and K balance compound fertilizer to mix well, covers plastic film moisturizing and stand 11.5 hours, fill 100 holes
Disk；Finally, the tomato seeds to show money or valuables one carries unintentionally through vernalization are sowed in seedling culture hole plate matrix, depth of planting 1cm, 1, every cave seed,
After planting use vermiculite earthing；Hole tray after planting is placed on the seedbed in seedling cultivation greenhouse, to make to keep the temperature, in seedbed upper berth underground heat
Line covers Small plastic shed, and 24 DEG C of daytime temperature or more, 19 DEG C of night temperature or so are kept in canopy.After emergence, film is opened in time, maintains daytime 22
~28 DEG C, 12~15 DEG C of night, will ventilate cooling in time when more than 29 DEG C, prevent seedling excessive growth.In seedling stage 3~5 leaf phase, prevent
Night temperature is too low, otherwise will affect bud differentiation, generates lopsided bud.If there is excessive growth trend, ventilate cooling in time, also sprayable
The paclobutrazol solution of 20mg/L is controlled.Temperature and control humidity are suitably reduced in order to cultivate strong sprout, before field planting, improve seedling
The anti-adversity ability of seedling；
2. field planting and field management: late Febuary, site preparation apply fertilizer, do furrow, and furrow are 1.25 meters wide, cover white mulch, and duplicate rows is fixed
It plants, seeding row spacing 0.4m × 0.55m, is colonized 2200 plants or so per acre；Base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage nitrogen
Fertilizer cannot be excessive, prevents plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, such as uses 1%~2% potassium dihydrogen phosphate
Solution foliage-spray.Netted melon only retains trunk, extract whole side shoots, timely bud picking；
3. selfing purifying: artificial pollination specifically: the plant of selection robust growth fruit type beauty and no disease and pests harm, selection are
The flower that open petal is not switched on gently is peelled off into anther cylinder emasculation with tweezers, is sure not gynoecium of damaging, same plant is won and is taken in
Its blooming flower piece pollinates to emasculation flower, and blooming flower piece is placed in the top of emasculation flower, and anther cylinder is directed at column cap,
It gently shakes, pollen can shed, and be covered with column cap, and sulfuric acid paper bag in rear enclosure of pollinating ties sack, and listing mark.Every fringe sits 2
~4 fruits, other flowers are all removed, to guarantee the plumpness of seed kernel；
4. selfed seed harvests: after pollination, tamato fruit selects fruit after fruit annesl through growth and development in 45~58 days
Good, the best in quality fruit harvesting of type, removes the peel, mashes, and 26~31 DEG C ferment 11.5 hours, so benefits from clear water washing and filtering, will
Obtained seed is dried in time, carries out Packaging and Labeling.
2, summer sowing sunshade plastic greenhouse cultivation breeding
1. seeding and seedling raising: carrying out seed presoaking and germinating before sowing, spring snow Red Indian race is put into fast in 54 DEG C of water mid-June
Speed stirring stops stirring when water temperature drops to 32 DEG C, and the 7.5h that soaks seed obtains what vernalization showed money or valuables one carries unintentionally then in 27 DEG C of at a temperature of vernalization
Tomato seeds；Matrix hole tray is prepared, by turf, perlite turf by volume: perlite=3:1 is mixed, and adds matrix quality thousand
/ mono- N P and K balance compound fertilizer mixes well, and covers plastic film moisturizing and stands 11.5 hours, fills 100 hole hole trays；Most
Afterwards, the tomato seeds to show money or valuables one carries unintentionally through vernalization are sowed, depth of planting 1cm, 1, every cave seed in seedling culture hole plate matrix, is sowed
Vermiculite earthing is used afterwards；Hole tray after planting is placed on the seedbed in seedling cultivation greenhouse, to make to cool down, seedling cultivation greenhouse should use sunshade
Net and Wet-curtain temperature reducing.Day temperature is maintained at 26~32 DEG C, 22 DEG C of night temperature or so, prevents seedling excessive growth.3~5 leaf phase in seedling stage,
It prevents temperature excessively high, otherwise will affect bud differentiation, generate lopsided bud.If there is excessive growth trend, ventilate cooling in time, may be used also
The paclobutrazol solution of sprinkling 22mg/L is controlled.In order to cultivate strong sprout, suitable control watering hardening is wanted before field planting, improves rice shoot
Anti-adversity ability；
2. field planting and field management: mid-July, site preparation apply fertilizer, do furrow, and furrow are 1.25 meters wide, cover black mulch film, and duplicate rows is fixed
It plants, seeding row spacing 0.4m × 0.55m, is colonized 2200 plants or so per acre；Base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage nitrogen
Fertilizer cannot be excessive, prevents plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, such as the potassium dihydrogen phosphate of sprinkling 1%~2%
Foliage-spray.Netted melon only retains trunk, extract whole side shoots, timely bud picking；
After field planting, laxative is primary within every 7~10 days, the pest and disease damages such as prevention chlorisis virus, canker, trialeurodes vaporariorum；
3. selfing purifying: artificial pollination specifically: the plant of selection robust growth fruit type beauty and no disease and pests harm, selection are
The flower that open petal is not switched on gently is peelled off into anther cylinder emasculation with tweezers, is sure not gynoecium of damaging, same plant is won and is taken in
Its blooming flower piece pollinates to emasculation flower, and blooming flower piece is placed in the top of emasculation flower, and anther cylinder is directed at column cap,
It gently shakes, pollen can shed, and be covered with column cap, and sulfuric acid paper bag in rear enclosure of pollinating ties sack, and listing mark.Every fringe sits 2
~4 fruits, other flowers are all removed, to guarantee the plumpness of seed kernel；
4. selfed seed harvests: after pollination, tamato fruit selects fruit after fruit annesl through growth and development in 45~58 days
Good, the best in quality fruit harvesting of type, removes the peel, mashes, and 26~31 DEG C ferment 11.5 hours, so benefits from clear water washing and filtering, will
Obtained seed is dried in time, carries out Packaging and Labeling.
2) breeding of male parent
Enlightening Pfennig, one dimensional infinite wall steamed dumpling with pork, mushrooms and bamboo shoots tomato, plant overgrow, and stalk is sturdy, and leaf color is black green, and growth potential is more prosperous.Anti- kind
Eggplant yellow leaf curl is viral (anti-TY).Fruit is round, fruit surface smooth, and fruit is uniform, continuous result energy, 200~250g of single fruit weight；Fruit
It is real hard, resistance to storage and transportation.
Using Di Fenni as source material, adds generation and the cold canopy of summer sowing to plant using 4 years early springs cold canopy, select height through 8 generations
For self-mating system male parent, the strain robust growth, anti-TY, continuous fruit setting ability is strong, fine work fruit rate is high, and the hardness of fruit is good, resistance to storage and transportation.
Breeding Process is identical as maternal Breeding Process.
3) crossbreeding
It is cross-breeding for self-mating system female parent and height for self-mating system male parent with height, obtains tomato combination, had
Body crossbreeding process is as follows:
1. sowing: selection fertile soil irrigates convenient plot, the production of hybrid seeds in greenhouse, and front and back is sealed 60 mesh or more and prevented
Worm net is obturaged, and prevents insect from entering.The ratio of Parent presses male parent: female parent=1:5 kind is planted, and Parent marks；
2. field management: the same production management of production of hybrid seeds field management；
3. pollination: artificial pollination specifically: gather and process pollen: after dew is dry on the day before pollination, being selected in paternal plant
Robust growth fruit type is beautiful and the plant of no disease and pests harm, and the picking same day is in full bloom, normotrophic flower, takes out anther and dries in the shade, temperature
Degree is no more than 35 DEG C, in order to avoid pollen aging and death.8~9 one-tenth of anther it is dry when, i.e., available pollen sieve sieve takes pollen, collects flower
Powder is set spare in a cool and dry place.Pollination: it is light that the flower tweezers that petal is not switched on will be opened on the healthy and strong maternal plant of selection
Anther cylinder emasculation gently is peelled off, is sure not gynoecium of damaging, dips the column cap that paternal pollen is gently applied to emasculation female parent flower with writing brush
On, sulfuric acid paper bag in rear enclosure of pollinating ties sack, and listing mark.Every fringe sits 3~4 fruits, other flowers are all removed,
To guarantee the plumpness of seed kernel；
4. the harvest of cenospecies: after pollination, tamato fruit selected after fruit annesl through growth and development in 45~58 days
Good, the best in quality fruit harvesting of fruit type, removes the peel, mashes, and 26~31 DEG C ferment 11.5 hours, so benefits from clear water washing and filtering,
Obtained seed is dried in time, carries out Packaging and Labeling.
Embodiment 3
The breeding method of the present embodiment, comprising the following steps:
1) maternal breeding
It is one dimensional infinite wall steamed dumpling with pork, mushrooms and bamboo shoots tomato that Syngenta Co., Ltd cultivates that spring snow is red, and plant overgrows, robust growth, and synthesis is disease-resistant
Property strong, anti-tomato yellow leaf curl virus (anti-TY), anti-root-knot nematode.Fruit pink, fruit surface smooth, fruit type is slightly flat, there is green fruit
Shoulder, 250~300g of single fruit weight, continuous bearing capacity are strong.Fruit soluble solids content is high, and fiber is few, sour-sweet than moderate, mouth
Feel.
It is red for source material with spring snow, add generation and the cold canopy of summer sowing to plant using 4 years early springs cold canopy, selects height through 7 generations
For self-mating system female parent, the strain plant strain growth is healthy and strong, and anti-TY, anti-root-knot nematode, continuous fruit setting ability is strong, and fruit is in good taste.
Specific Breeding Process is specific as follows:
1, early spring cold canopy additive selection
1. seeding and seedling raising: spring snow Red Indian race is put into 56 DEG C of water firstly, carrying out seed presoaking and germinating before sowing by mid-January
In stir rapidly, when water temperature drops to 29 DEG C, stop stirring, seed soaking 9h obtain vernalization then in 27 DEG C of at a temperature of vernalization and show money or valuables one carries unintentionally
Sponge gourd seed；Matrix hole tray is prepared, by turf, perlite turf by volume: perlite=3:1 is mixed, and adds matrix quality
Millesimal N P and K balance compound fertilizer mixes well, and covers plastic film moisturizing and stands 13 hours, fills 100 hole hole trays；Most
Afterwards, the tomato seeds to show money or valuables one carries unintentionally through vernalization are sowed in seedling culture hole plate matrix, depth of planting 0.8cm, 1, every cave seed is broadcast
Vermiculite earthing is used after kind；Hole tray after planting is placed on the seedbed in seedling cultivation greenhouse, to make to keep the temperature, on seedbed upper berth hot line
Small plastic shed is covered, 26 DEG C of daytime temperature or more, 19 DEG C of night temperature or so are kept in canopy.After emergence, open film in time, maintain daytime 20~
26 DEG C, 11~16 DEG C of night, will ventilate cooling in time when more than 28 DEG C, prevent seedling excessive growth.In seedling stage 3~5 leaf phase, prevent night
Temperature is too low, otherwise will affect bud differentiation, generates lopsided bud.If there is excessive growth trend, ventilate cooling in time, also sprayable
The paclobutrazol solution of 20mg/L is controlled.Temperature and control humidity are suitably reduced in order to cultivate strong sprout, before field planting, improve seedling
The anti-adversity ability of seedling；
2. field planting and field management: late Febuary, site preparation apply fertilizer, do furrow, and furrow are 1.25 meters wide, cover white mulch, and duplicate rows is fixed
It plants, seeding row spacing 0.4m × 0.55m, is colonized 2200 plants or so per acre；Base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage nitrogen
Fertilizer cannot be excessive, prevents plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, such as uses 1%~2% potassium dihydrogen phosphate
Solution foliage-spray.Netted melon only retains trunk, extract whole side shoots, timely bud picking；
3. selfing purifying: artificial pollination specifically: the plant of selection robust growth fruit type beauty and no disease and pests harm, selection are
The flower that open petal is not switched on gently is peelled off into anther cylinder emasculation with tweezers, is sure not gynoecium of damaging, same plant is won and is taken in
Its blooming flower piece pollinates to emasculation flower, and blooming flower piece is placed in the top of emasculation flower, and anther cylinder is directed at column cap,
It gently shakes, pollen can shed, and be covered with column cap, and sulfuric acid paper bag in rear enclosure of pollinating ties sack, and listing mark.Every fringe sits 3
~4 fruits, other flowers are all removed, to guarantee the plumpness of seed kernel；
4. selfed seed harvests: after pollination, tamato fruit selects fruit after fruit annesl through growth and development in 47~60 days
Good, the best in quality fruit harvesting of type, removes the peel, mashes, and 24~29 DEG C ferment 12.5 hours, so benefits from clear water washing and filtering, will
Obtained seed is dried in time, carries out Packaging and Labeling.
2, summer sowing sunshade plastic greenhouse cultivation breeding
1. seeding and seedling raising: late June carries out seed presoaking and germinating before sowing, spring snow Red Indian race is put into fast in 56 DEG C of water
Speed stirring stops stirring when water temperature drops to 29 DEG C, and the 9h that soaks seed obtains kind that vernalization shows money or valuables one carries unintentionally then in 27 DEG C of at a temperature of vernalization
Eggplant seed；Matrix hole tray is prepared, by turf, perlite turf by volume: perlite=3:1 is mixed, and matrix quality thousand is added to divide
One of N P and K balance compound fertilizer mix well, cover plastic film moisturizing stand 13 hours, fill 100 hole hole trays；Finally, will
The tomato seeds to show money or valuables one carries unintentionally through vernalization are sowed in seedling culture hole plate matrix, depth of planting 0.8cm, and 1, every cave seed is after planting used
Vermiculite earthing；Hole tray after planting is placed on the seedbed in seedling cultivation greenhouse, to make to cool down, seedling cultivation greenhouse should use sunshade net and
Wet-curtain temperature reducing.Day temperature is maintained at 24~29 DEG C, 19 DEG C of night temperature or so, prevents seedling excessive growth.In seedling stage 3~5 leaf phase, prevent
Temperature is excessively high, otherwise will affect bud differentiation, generates lopsided bud.If there is excessive growth trend, ventilate cooling in time, also sprayable
The paclobutrazol solution of 18mg/L is controlled.In order to cultivate strong sprout, suitable control watering hardening is wanted before field planting, improves the anti-of rice shoot
Inverse ability；
2. field planting and field management: mid or late July, site preparation apply fertilizer, do furrow, and furrow are 1.25 meters wide, cover black mulch film, duplicate rows
Field planting, seeding row spacing 0.4m × 0.55m are colonized 2200 plants or so per acre；Base manure is sufficient, and seedling stage can suitably apply and mention seed manure, but seedling stage
Nitrogenous fertilizer cannot be excessive, prevents plant excessive growth.After the first tongue fur bears fruit, phosphorus potash fertilizer is imposed, such as the biphosphate of sprinkling 1%~2%
Potassium foliage-spray.Netted melon only retains trunk, extract whole side shoots, timely bud picking；
After field planting, laxative is primary within every 7~10 days, the pest and disease damages such as prevention chlorisis virus, canker, trialeurodes vaporariorum；
3. selfing purifying: artificial pollination specifically: the plant of selection robust growth fruit type beauty and no disease and pests harm, selection are
The flower that open petal is not switched on gently is peelled off into anther cylinder emasculation with tweezers, is sure not gynoecium of damaging, same plant is won and is taken in
Its blooming flower piece pollinates to emasculation flower, and blooming flower piece is placed in the top of emasculation flower, and anther cylinder is directed at column cap,
It gently shakes, pollen can shed, and be covered with column cap, and sulfuric acid paper bag in rear enclosure of pollinating ties sack, and listing mark.Every fringe sits 3
~4 fruits, other flowers are all removed, to guarantee the plumpness of seed kernel；
4. selfed seed harvests: after pollination, tamato fruit selects fruit after fruit annesl through growth and development in 47~60 days
Good, the best in quality fruit harvesting of type, removes the peel, mashes, and 24~29 DEG C ferment 12.5 hours, then uses clear water washing and filtering, will
Obtained seed is dried in time, carries out Packaging and Labeling.
2) breeding of male parent
Enlightening Pfennig, one dimensional infinite wall steamed dumpling with pork, mushrooms and bamboo shoots tomato, plant overgrow, and stalk is sturdy, and leaf color is black green, and growth potential is more prosperous.Anti- kind
Eggplant yellow leaf curl is viral (anti-TY).Fruit is round, fruit surface smooth, and fruit is uniform, continuous result energy, 200~250g of single fruit weight；Fruit
It is real hard, resistance to storage and transportation.
Using Di Fenni as source material, adds generation and the cold canopy of summer sowing to plant using 4 years early springs cold canopy, select height through 8 generations
For self-mating system male parent, the strain robust growth, anti-TY, continuous fruit setting ability is strong, fine work fruit rate is high, and the hardness of fruit is good, resistance to storage and transportation.
Breeding Process is identical as maternal Breeding Process.
3) crossbreeding
It is cross-breeding for self-mating system female parent and height for self-mating system male parent with height, obtains tomato combination, had
Body crossbreeding process is as follows:
1. sowing: selection fertile soil irrigates convenient plot, the production of hybrid seeds in greenhouse, and front and back is sealed 60 mesh or more and prevented
Worm net is obturaged, and prevents insect from entering.The ratio of Parent presses male parent: female parent=1:6 kind is planted, and Parent marks；
2. field management: the same production management of production of hybrid seeds field management；
3. pollination: artificial pollination specifically: gather and process pollen: after dew is dry on the day before pollination, being selected in paternal plant
Robust growth fruit type is beautiful and the plant of no disease and pests harm, and the picking same day is in full bloom, normotrophic flower, takes out anther and dries in the shade, temperature
Degree is no more than 35 DEG C, in order to avoid pollen aging and death.8~9 one-tenth of anther it is dry when, i.e., available pollen sieve sieve takes pollen, collects flower
Powder is set spare in a cool and dry place.Pollination: it is light that the flower tweezers that petal is not switched on will be opened on the healthy and strong maternal plant of selection
Anther cylinder emasculation gently is peelled off, is sure not gynoecium of damaging, dips the column cap that paternal pollen is gently applied to emasculation female parent flower with writing brush
On, sulfuric acid paper bag in rear enclosure of pollinating ties sack, and listing mark.Every fringe sits 3~4 fruits, other flowers are all removed,
To guarantee the plumpness of seed kernel；
4. the harvest of cenospecies: after pollination, tamato fruit selected after fruit annesl through growth and development in 47~60 days
Good, the best in quality fruit harvesting of fruit type, removes the peel, mashes, and 24~29 DEG C ferment 12.5 hours, so benefits from clear water washing and filtering,
Obtained seed is dried in time, carries out Packaging and Labeling.
The resulting seed of above three embodiments is planted experimentally, is obtained to planting experimentally after result counts: above-mentioned implementation
The tamato fruit pericarp that the seed of example is born after planting experimentally is colourless, and fruit is round, and green fruit shoulder is medium, and fruit tip circle is flat, fruit shoulder dimple, fruit
Face is smooth, there is micro- rib, and 230~260 grams of single fruit weight.Fruit color depth powder, uniformly, taste is sour-sweet, succulence, and hardness is preferable for fruit,
Very resistance to storage and transportation.During the seed of above-described embodiment is planted experimentally, tomato anti-TMV, anti-tomato yellow leaf curl virus during the growth process
Sick (TY-1 gene loci), in anti-root-knot nematode (Mi gene loci), anti mosaic virus of tomato.Per mu yield can reach 5000~
7500kg。
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of breeding method of the fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste, it is characterised in that the following steps are included:
1) maternal breeding: using spring snow Fructus Lycopersici esculenti as source material, with anti-root-knot nematode (Mi), fruit is in good taste, rediance,
Robust growth ability of continuously bearing fruit is breeding objective by force, using continuous selfing and molecular marker-assisted selection method, through 7 generation single plants
Selection carries out mixing and reserves seed for planting, and obtains high for self-mating system female parent；
2) breeding of male parent: using Di Fenni tomato as source material, with the resistance to storage and transportation of fruit, fruit type is good, plant strain growth is healthy and strong, anti-kind
Eggplant yellow leaf curl virus is breeding objective, is obtained using continuous selfing and molecular marker-assisted selection method through 4 generation Single-plant selections
4 generation strains are obtained, are selected using continuous selfing and molecular marker-assisted selection method through 2 generation single plants in conjunction with resisting etiolation curve leaf disease virus
It selects, obtains 6 generation strains, most remove hybrid strain through 2 generation Single-plant selections afterwards, carry out mixing and reserve seed for planting, obtain height for self-mating system male parent；
3) crossbreeding: maternal and male parent is cross-breeding, the seed of the fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste is obtained；
Breeding plants breeding using early spring cold canopy and the cold canopy of summer sowing in the step 1) and step 2).
2. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 1, which is characterized in that institute
State early spring cold canopy breeding, comprising the following steps:
1. seeding and seedling raising: mid-January, the seed that vernalization is showed money or valuables one carries unintentionally matrix hole plate seedling growth；
2. field planting and field management: late Febuary, to tomato seedling length to 4~5 true leaves, field planting, built is cultivated, Netted melon,
Every plant is stayed 4~6 tongue fur fruits, and 2 leaf toppings are stayed at the top of last Honoka sequence；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4, separates from the second generation
Group carries out Single-plant selection, and the method selected is Marker-assisted selection, then in fructescence according to the exterior quality of fruit
Single-plant selection is preferentially carried out with nutritional quality；
4. selfed seed harvests: to Fruit Ripening of Tomato, taking fruit remove the peel, mash, fermenting, clean filtering, after sunning, being selfed
Kind.
3. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 1, which is characterized in that institute
The cold canopy of the summer sowing stated plants breeding, comprising the following steps:
1. sowing: in mid or late June, the seed that vernalization is showed money or valuables one carries unintentionally matrix hole plate seedling growth；
2. field planting and field management: mid or late July, field planting, built was cultivated, Netted melon after seedling length to 4~5 true leaves,
Every plant is stayed 4~6 tongue fur fruits, and 2 leaf toppings are stayed at the top of last Honoka sequence；
3. being selfed Single-plant selection: to just open flower artificial supplementary pollination, every fringe stays fruit 3~4, separates from the second generation
Group carries out Single-plant selection, and the method selected is Marker-assisted selection, then in fructescence according to the exterior quality of fruit
Single-plant selection is preferentially carried out with nutritional quality；
4. selfed seed harvests: to Fruit Ripening of Tomato, fruit being taken to remove the peel, mash, ferment, after cleaning filtering, sunning, be selfed
Kind.
4. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 2 or 3, feature exist
In, in the sowing step, vernalization shows money or valuables one carries unintentionally the stirring the preparation method comprises the following steps: tomato seeds are placed in 50~55 DEG C of water of seed,
25~35 DEG C of stopping stirrings being dropped to water temperature, soak seed 5~8h, and then vernalization obtains at 28~30 DEG C.
5. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 2 or 3, feature exist
In, in the sowing step, the method for nursery are as follows: the every cave of hole tray is put into the seed that 1 vernalization shows money or valuables one carries unintentionally, and hole tray after planting is set
In on vinyl house or the indoor seedbed of sunlight, 25 DEG C of daytime temperature or more, 16~20 DEG C of night temperature are kept in canopy；After emergence, maintain
20~25 DEG C of daytime, 10~15 DEG C of night.
6. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 5, it is characterised in that: institute
It states in the method for nursery, further includes thering is the paclobutrazol solution for spraying 20mg/L when tomato seedling has excessive growth trend to be controlled
Step.
7. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 2 or 3, feature exist
In, in the field planting and field management step, the method for field planting are as follows: be colonized in furrow face duplicate rows, furrow wide 1.25m, seeding row spacing 0.4m
× 0.55m, 2200 plants/acre of plantation density.
8. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 2 or 3, feature exist
In: in the selfing Single-plant selection step, the method for artificial supplementary pollination uses the anther of the just open flower in same strain
On take pollen, or be derived from the pollen that oneself takes, carry out supple-mentary pollination, every fringe stays fruit 3~4.
9. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 1, which is characterized in that institute
State step 3) crossbreeding, comprising the following steps:
1. space isolation and sowing: Parent ratio presses paternal plant: planting maternal plant=1:4~8 kind；
2. field management: florescence removal maternal plant pollinates and open flower not in time；
3. pollination: selecting to open and flower that petal is not switched on peels off anther cylinder emasculation on female parent, take the pollen in male parent
It awards on the gynoecium of maternal emasculation flower, every fringe sits 3~4 fruits；
4. cenospecies harvests: to Fruit Ripening of Tomato, taking fruit remove the peel, mash, fermenting, clean filtering, dry, hybridized
Kind.
10. the breeding method of one kind fine work of resistance to storage and transportation steamed dumpling with pork, mushrooms and bamboo shoots tomato in good taste according to claim 9, which is characterized in that
The pollination step the following steps are included:
1. adopting powder: after dew is dry on the day before pollination, selecting robust growth fruit type beautiful from paternal plant and no disease and pests harm
Plant, the picking same day is in full bloom, normotrophic flower, takes out anther and dries in the shade, temperature is no more than 35 DEG C；
2. powder processed: when 8~9 one-tenth of anther dry, take pollen with pollen sieve sieve, and collect pollen set it is spare in a cool and dry place；
3. pollination: selecting that petal emasculation will be opened on healthy and strong maternal plant, dip 2. pollen that step is collected with writing brush and smear
On flower column cap, every fringe sits 3~4 fruits, and other flowers are all removed.
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| CN111903448A (en) * | 2020-08-24 | 2020-11-10 | 云南省农业科学院生物技术与种质资源研究所 | Chili plant cultivation method |
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| CN114009335A (en) * | 2021-12-09 | 2022-02-08 | 武汉楚为生物科技有限公司 | Tomato breeding method |
| CN114568296A (en) * | 2022-02-28 | 2022-06-03 | 武汉楚为生物科技有限公司 | Method for cultivating multi-resistance crack-resistant tomato variety |
| CN115669534A (en) * | 2021-11-26 | 2023-02-03 | 天津市农业科学院 | Tomato breeding and planting method with molecular marker assistance and artificial inoculation of pathogenic bacteria |
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| CN111903448A (en) * | 2020-08-24 | 2020-11-10 | 云南省农业科学院生物技术与种质资源研究所 | Chili plant cultivation method |
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| CN115669534A (en) * | 2021-11-26 | 2023-02-03 | 天津市农业科学院 | Tomato breeding and planting method with molecular marker assistance and artificial inoculation of pathogenic bacteria |
| CN115669534B (en) * | 2021-11-26 | 2024-02-23 | 天津市农业科学院 | Molecular marker assisted and artificial inoculation pathogenic bacteria tomato breeding and planting method |
| CN114009335A (en) * | 2021-12-09 | 2022-02-08 | 武汉楚为生物科技有限公司 | Tomato breeding method |
| CN114568296A (en) * | 2022-02-28 | 2022-06-03 | 武汉楚为生物科技有限公司 | Method for cultivating multi-resistance crack-resistant tomato variety |
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Denomination of invention: A breeding method of fine powdered tomato with good taste and storage and transportation resistance Effective date of registration: 20220630 Granted publication date: 20200717 Pledgee: Industrial Bank Limited by Share Ltd. Wuhan branch Pledgor: Wuhan Chuwei Biotechnology Co.,Ltd. Registration number: Y2022420000211 |