CN104335891A - A cultivation method of new strawberry species - Google Patents
A cultivation method of new strawberry species Download PDFInfo
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- CN104335891A CN104335891A CN201310331481.XA CN201310331481A CN104335891A CN 104335891 A CN104335891 A CN 104335891A CN 201310331481 A CN201310331481 A CN 201310331481A CN 104335891 A CN104335891 A CN 104335891A
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Abstract
The invention relates to the technical field of agriculture bioengineering and particularly relates to a cultivation method of new strawberry species. The method includes steps of: pushing petals aside of female parent flowers in a big bud stage; pulling up all the stamens; picking male parent flowers to pollinate the female parent flowers; covering the flower heads with paper bags after pollination; removing the paper bags when receptacles begin expand after 10-15 d and bear young fruits; picking mature hybrid fruits after 30-40 days successively; cleaning seeds, air-drying; putting the seeds into a refrigerator; refrigerating at 1-3 DEG C and keeping for further use; sieving sandy soil with a sieve having a size of 1-2.5 mm, autoclaving, disinfecting and seeding; and after the seeds survive, planting seedlings having 4-5 leaves to a greenhouse, accelerating culture, and performing strain evaluation screening in fields to obtain the screened new strawberry species. By adoption of a crossbreeding method, strawberry individuals with different excellent properties are hybridized, and through genetic recombination, individuals with heterosis are obtained.
Description
Technical field
The present invention relates to agro-biological engineering technical field, particularly relate to a kind of New Strawberry Variety breeding method.
Background technology
The registered kind of world strawberry more than 3000, wherein the U.S., Japan are Strwberry Breeding big countries, and kind incubation quantity is many, updating decision, is in Strwberry Breeding leading position, the world.
Due to China's strawberry cultivars be mostly introduce world Two decades years before kind (world-class new quality product kind can not be introduced in time because China does not still carry out kind Protection Code); although whole nation strawberry production area reaches 1,700,000 mu; rank first in the world; but Yield of Strawberry on average every mu only have 1-1.5 ton; 1/2nd output of the U.S., Spain and Japan; and bad, disease resistance and storage tolerance are poor; strawberry commodity value and export volume are affected; therefore from these aspects, the Shang Shu world of China strawberry production " micro state ".
Donggang City is that the whole nation is introduced a fine variety strawberry and continuity production development the earliest and forms the strawberry production base of strong industry, but it is the same with the whole nation, quote external kind for many years, greenhouse by solar heat kind of planting is divided into two classes always: a class is the American-European kind (Du Kela, Tu Dela etc.) that output is high, poor quality, disease resistance strong, fortune storage property is good; One class be best in quality, taste-aromatic but disease resistance poor, yield poorly, not storage tolerance Japan cultivars (a chapter Ji, Feng Xiang, precious hand over early raw etc.); These kinds be all last century the nineties from the external kind introduced, although each tool high yield between kind, high-quality, storage tolerance, the different advantageous characteristic feature characteristic such as disease-resistant, but because strange land breeding condition limit, the ecological reproductive condition such as temperature, light, water, soil of Dandong can not be adapted to completely, limited to the larger raising of Donggang City's Yield of Strawberry, quality and benefit.Therefore, cultivating the new varieties being applicable to this area working condition, for peasant and Strawberry industryization development provide the optimum kind being superior to existing production kind, is very necessary and very urgent tackling of key scientific and technical problems task.
Summary of the invention
(1) technical problem that will solve:
The present invention will cultivate that resting stage is shallow, disease resistance is strong, output is high, best in quality, hardness good, kind is facilitated in the greenhouse of fruit shape U.S..
(2) technical scheme
The petal that 1. female parent is in large flower bud phase flower by a kind of New Strawberry Variety breeding method is pushed aside, pulls out whole stamen,
2. win male parent flower to pollinate to female parent, with paper bag, fresh idea packaged after pollination,
3. when 10-15d holder starts to expand and bears young fruit, paper bag is removed, successively the hybridization fruit of maturation is taken off after 30-40 days, by wash seeds, dry after be put in refrigerator, refrigerate stand-by under 1-3 DEG C of condition;
4. get sandy loam, sow after autoclaving sterilization after crossing 1-2.5mm sieve;
5. after surviving, the young plant Miao Dingzhi that grows directly from seeds possessing 4-5 sheet leaf is carried out field strain Evaluating and screening to green house forcing culture, obtain screening new varieties.
When pulling out whole stamen, column cap can not be hurt; Put paper bag immediately after pulling out whole stamen until open when pollinating, with paper bag, fresh idea is packaged immediately after pollination.
Pollination is the flower of petal male parent do not launched, and collects in clean culture dish, and the column cap of maternal emasculation is formerly smeared gently, or direct tweezers are pulled up male parent flower pesticide and are put on the column cap of maternal gynoecium.
Sowing be by sterilizing after soil be dispensed in the middle of flowerpot, evenly play wherein by seed, seed covers the thin soil of one one-tenths 2-3mm, overlay film moisturizing of spraying water, moves on in green house and survives the winter, move to nursery after seedling winter.
Before sowing, soil soaks in basin, then taking-up control is dry; Water spray is evenly squirted by top layer earthing with sprayer.
Autoclaving described in step 4 is sterilizing 15min under 0.1Mpa temperature 120 DEG C of conditions.
The most in good time phase of pollination is April, and the most in good time phase of sowing is the September then obtaining seed.
Advantage of the present invention
Take cross breeding method, the strawberry individuality of different merit is hybridized, by genetic recombination, obtain and there is heterotic individuality.Advantage: the bion that according to prediction, the excellent genes restructuring be positioned on two organisms can be obtained merit set, realize directive breeding, and the new individual characters produced is more stable.The method is simple to operate, and purpose is strong.
Cultivate in hybridization in the process of new varieties, select the parents with merit, make the merit probability of the comprehensive parents of offspring individuals large, hybrid vigour is obvious, easily obtains desirable offspring.Emasculation bagging before pollination is to ensure that offspring's parental trait is excellent pure; The method that pollinating process is taked is to gather in the crops well-developed hybridization fruit, obtains the seed of the sufficient full maturation of quantity; The clear water extraction of true seed, the cold storage of refrigerator, the sterilization processing of sowing seed soil and the precision management of seedling, to improve true seed emergence rate, to obtain the seedling number of sufficient amount, thus improve the probability that there is comprehensive excellent genes individuality and occur, realize precocity, high-quality, high yield, disease-resistant breeding objective.
Embodiment
For selecting the improved seeds of precocity, high yield, high-quality, disease-resistant suitable forcing culture, the high yield that the fine quality proterties taking comprehensive Japan cultivars to have and American-European kind have and disease resistance trait hybridization obtain new quality product kind.Be used in the main object that kind that local forcing culture does very well configures as hybrid combination.
With reference to domestic and international Strwberry Breeding skill, in conjunction with our experience for many years, formulate strawberry crossbreeding program.The quality of the suitable Donggang City's greenhouse culture of choosing preferably Japan " a chapter Ji " be maternal, and disease resistance is comparatively strong, good " Spain " Du Kela of Fruit Economic Traits is male parent, hybridizes.9-11 point in morning April 13 (fine day) in 1998, in strawberry research institute science and technology Demonstration Base greenhouse, carry out field hybridization.Selected robust growth, kind pure " a chapter Ji " plant are as maternal acceptor, and pushed aside by the petal being in large flower bud phase flower with tweezers, pull out whole stamen, attention can not hurt column cap, puts paper bag immediately after emasculation.Win male parent petal not launch and the filigree of stamen is sturdy, the flower of flower pesticide obesity, collect in clean culture dish, the column cap of maternal emasculation is formerly smeared gently, or direct tweezers are pulled up male parent flower pesticide and are put on the column cap of maternal gynoecium, rear paper bag of pollinating packages fresh idea, hangs up the label with father, parental varieties title and pollination date simultaneously.When 10-15d holder starts to expand and bears young fruit (young fruit nearly unguiculus lid is large), then paper bag is removed.Fully ripe hybridization fruit takes off by 17-21 day in May successively, does wash seeds, dries process, obtain true seed 260 altogether, make a record, be put in refrigerator, refrigerate under 1-3 DEG C of condition.Sowing in September then, get the sandy loam of safe no drug residue, cross the laggard horizontal high voltage sterilizing of dusting cover (0.1Mpa 120 DEG C of sterilizing 15min) sterilization, then the fine earth after sterilization is dispensed in the middle of flowerpot, before sowing, flowerpot soil is put into and soaks in basin, take out control more dry, even seed is play wherein, spray water after seed covering the thin soil of one one-tenth 2-3mm, water spray is evenly squirted by top layer earthing with the little sprayer of Domestic portable (2L), and mist flower wants thin, carefully seed spray can not be rushed out, overlay film moisturizing, manages with delicacy.Move on to winter in green house and survive the winter, the strain of investigation seedling number 208.Seedling is moved to nursery by April 6 next year, survives 165 strains, be set as 165 strains to investigation on May 20.The young plant Miao Dingzhi that grows directly from seeds possessing 4-5 sheet leaf is carried out field strain Evaluating and screening to green house forcing culture by September 10.Fruit harvest in June, 2000 terminates.In 165 strains of field planting code name ZA8-1 with precocious, individual large, fruit colour is gorgeous, mouthfeel good, disease-resistant, hardness large, high yield, the advantages such as disease resistance is strong are as choosing.Carry out seeding propagation then, at the beginning of 9 months, ZA8-1 is colonizated in greenhouse by solar heat, with " Feng Xiang ", " a chapter Ji ", " Du Kela " for contrast, proceed field evaluation final election.Mainly detailed investigation and research are carried out to its botanical character, biological property, phenophase, fruit properties, yielding ability, disease resistance.Until June calendar year 2001, fruit harvesting terminated.Finally from 165 hybridization seedlings, select the U.S. kind of red reality (primary ZA8-1).
2001.9-2003.9 facilitates main breed " Feng Xiang ", " a chapter Ji ", " Du Kela " for contrast with greenhouse, greenhouse by solar heat multispots trial is carried out on the original seed demonstration of strawberry research institute of Donggang City field, Qian Yang town, Yi Quan town, Temple of the Dragon King town and other places, " red real beautiful " is good in each point performance, and proterties is stablized.Period carries out seeding propagation by detoxication and tissue culture, is introduced a fine variety on our province and Xinjiang, Jilin, Heilungkiang and other places and applies.Strawberry research institute test site result of the test shows, the beautiful 667m of red reality
2output is respectively than a chapter Ji, Du Kela, Feng Xiang volume increase 93.63%, 33.34%, 107.22%.The U.S. common manifestation high yield and high quality of red reality of all the other tests, demonstration pilot project and high benefit.(attached: Donggang City strawberry red reality of tested point U.S. feature application form 1-4 in 2003).Nikkei in January 10 Liaoning Province crop varietal approval committee name in 2005, be bred as red solid beautiful strawberry new varieties, the same year is by the qualification of Dandong city-level scientific and technological achievement.Domestic expert appraisal is thought, the U.S. new varieties of red reality " 1, disease resistance is strong; 2, yielding ability is good; 3, best in quality; 4, storage tolerance; 5, hothouse production is applicable to ", " this achievement level is reached advanced world standards "; Thereafter apply in Donggang City and national many places; This achievement is cited as Dandong City's scientific and technological progress second prize for 2009.
Hong Shimeishi China registration self-fertile the 32nd New Strawberry Variety, " having filled up China's self-fertile greenhouse high-quality, high yield, disease-resistant variety blank " applies the maximum domestic kind of area up to now.
In experiment and demonstration production process, in conjunction with the U.S. habit feature of red reality, sum up corresponding supporting high quality and yield cultivation techniques.
Variety characteristic and technical essential
(1) kind morphological feature
The strong and healthy golden mean of the Confucian school of the U.S. growing way of red reality, blade subcircular, dark green leaf color is glossy, and the strain number of sheets is than Feng Xiang and the few 2-3 sheet of a chapter Ji, and plant height 28-30cm, strain state half the first transaction of a day's business inflorescence is lower than blade face, and the thick flower bud of handle is large, Post flowering 30-40 days fruit complete ripeness.Stolon pumping ability is strong, and reproduction coefficient is similar to Du Kela higher than Feng Xiang and a chapter Ji, and when numerous seedling stage, temperature was more than 30 DEG C, maternal plant lobus cardiacus is slightly in distorted shape (but sub-seedling does not have this shape).Flower bud differentiation is more Zao than Du Kela, and the maturing stage shifts to an earlier date about 25 days, easily forms bud after especially sub-seedling goes out root.Resting stage is shallow, and namely less than 7 DEG C 150 hours accumulated temperature amounts meet broken dormancy.
(2) suitable cultivation form
The U.S. kind of red reality is suitable for northern greenhouse or southern Small plastic shed forcing culture, can gather in the crops for a long time from mid-December in Operation in Dandong Area to June in next year.But the north early spring large cold canopy Semi-forcing culture or outdoor cropping growing way excessively prosperous, flower_bud formation is few, and output is not high.
(3) high quality and yield cultivation techniques code
1, seedling
Select detoxication and tissue culture former sapling multiplication production seedling, numerous seedling field answers that soil fertility is fertile, permeability good, row irrigation is convenient.Field planting at the beginning of 4 months, every strain seedling takes up an area 0.6m
2, the sub-seedling of every strain breeding 30-40 strain then, to breed sub-seedling then as production seedling, quality standard is for there being 5-7 sheet expanded leaves, and petiole is sturdy, thick more than the φ 0.8cm of rhizome, and coring is flourishing, without damage by disease and insect.
2, soil and Fertilization
In greenhouse by solar heat, field requires that soil property is fertile, and short texture, the content of organic matter is more than 1.5%.667m
2execute decomposed manure 3000-4000kg, diammonium phosphate 15kg, potassium dihydrogen phosphate 10kg.Base manure, with soil deeptillage 30cm whole layer placement of fertilizer, ploughs the thin rear ridging of even rake, row spacing 85-90cm, ridge height 25-30cm (the low then ridge of subterranean water level is short, and subterranean water level height then ridge is high).
3, planting density, the degree of depth, direction
At the beginning of resident time north late August to 9 month, south mid-September to early October, 667m
2to plant 9000-10000 strain, large-ridge double-row, little line-spacing 25-30cm, spacing in the rows 15-17cm.
During field planting, the rice shoot back of a bow is beneficial to flowers and fruits ventilation and daylighting towards furrow.Plant more than the seedling degree of depth and do not bury the heart, under not crab rooting be advisable.For alleviating slow seedling and improving survival rate, sunshade net is utilized to be advisable.
4, canopy film covers front field management
Seedling will water after planting in time, will keep ground moistening before slow seedling.When young leaves normally launches, during phenomenon that have in the morning " guttation ", show that new root produces, irrigation times can reduce, and carries out intertill and clean tillage.The stolon will extractd dead leaf after slow seedling in time and just occur.
5, canopy film and mulch film is covered
The time of northern area covering with plastic film and canopy film, at early and middle ten days in October, namely carries out during daily mean temperature less than 10 DEG C.For increasing the illuminance in greenhouse, the good polyvinyl chloride Anti-fogging Film of light transmission is adopted to be advisable.Unregistered land film selected by mulch film, to reduce Weed infestation.
6, gibberellin uses
Red real U.S.A does not generally use gibberellin.Cause seedling dormancy comparatively dark as postponed because of upper greenhouse, after greenhouse heating, can spray 5ppm concentration gibberellin a week, every strain consumption 5 milliliters, emphasis is sprayed on Miao Xinchu.Gibberellin consumption can not be excessive, otherwise cause excessive growth and early ageing.
7, flower and fruit management
Red real U.S.A has stronger habit of yielding positive results continuously, must carry out flower and fruit thinning management talent and ensure that fruit is large, poppyhead sequence no matter several stay 3-4 fruit, stay 3 fruits after second batch of inflorescence, the 3rd batch afterwards inflorescence stay 3 fruits.Removing the flowers and fruits time carries out early, avoids losing nutrient.
8, honeybee pollination
Start to put honeybee supplementary pollination in a suitable place to breed in the early flowering season (5% blooms), honeybee amount is advisable with every strain one honeybee.In order to prevent honeybee from fleeing, gauze to be added a cover in ventilation.Should note when pesticide grown and chemical fertilizer in canopy protecting honeybee.
9, temperature adjusting
Temperature indoor temperature height will adapt to strawberry growth habit, and before both having shown the flower bud phase vegetative growth phase, temperature was 28-30 DEG C, and bud stage 25-28 DEG C, fruit expanding period is advisable with 20-25 DEG C.Whole night breeding time, minimum temperature can not lower than 6 DEG C.Fruit expanding period day and night temperature is not more than 12 DEG C and is conducive to increasing fruit pol and hardness.
10, humidity regulation
Moisture management is the important step meeting strawberry physiological requirements and reduce damage by disease and insect.Water capacity requires flower bud differentiation period 60%, and vegetative growth phase 70%, flowering fruit bearing stage 80% as well.Temperature indoor air relative humidity is advisable with less than 70%, and humidity is excessive will ventilate in time.Irrigation method is best with drip irrigation, also the middle shallow ridges duty of little duplicate rows can fill with, does not advocate major groove flood irrigation.Space observation in plant and canopy, when taking off curtain morning, plant leaf has guttation, does not have fog as well in canopy, and blade does not have " guttation " may soil lack of water, canopy intrinsic fog large may soil partially flooded.Red real beautiful gathering a few days ago and can not pouring water, to ensure the hardness of fruit and fine quality.
11, Nutrition Management
The Strawberry in Greenhouse using sufficient base fertilizer is not advocated and is imposed solid chemical fertilizer, once fertilizer deficiency, supplements by drip irrigation or foliar application.Drip irrigation imposes the concentration of urea or other chemical fertilizer can not higher than 0.3%.The foliage fertilizer usable highly effective liquid compoiste fertilizer sprayed, sprayed once every 10 ~ 15 days.
12, the extermination of disease and insect pest
Although red reality U.S. Variety Disease-resistance insect pest ability is strong, also the integrated control of very important various damage by disease and insect, adheres to relying mainly on prevention, and is auxiliary principle to control, and reduces chemical pesticide as far as possible and uses, based on agronomic measures and low silkworm raiser's medicine.If any Avermectin 2000 times or 15% pyridaben 2000 times of two spotted spider mite spray 1.8%.Trialeurodes vaporariorum, aphid use 10% Imidacloprid 1500 or 50% Aphox 2500 times.Gather and discontinue medication for first 15 days, fumigate with fumicants between picking time.
13, gather, pack and market
Harvest maturity is foundation with market sale, and finding a good sale in gathers ripely to be advisable with eight points, and nearly pin to be ripely advisable with 9 points.Collecting time is to be advisable during blade dew eliminating in early morning and before the dusk.
Packaging can adopt bubble chamber or plastic packing box, and bubble chamber generally fills the two-layer fruit of pendulum in 1-2kg fresh fruit case, and plastics package is box-packed can be quantitative by number.Because the U.S. hardness of red reality is better, shelf life is long, can long distance shipping and marketing.
14, flowers and fruits and blade manage continuously
The U.S. growing way of red reality is strong and healthy, and continuous bearing capacity is strong, to last flowering fruit bearing stage from yielding positive results, will carry out flower thinning, fruit thinning work all the time, is thinning out residual flower, senior flower and stubble in time, is thinning out disease fruit, malformed fruit 2, reaches the object of high-quality, high yield.Remove Lao Ye, sick leaf simultaneously, promote that young leaves is sprouted, for fruit development provides sufficient nutrition.
Innovative point and useful achievement
By the strawberry that the present invention produces---red real U.S.A has following characteristics
1, disease resistance is strong.High mildew-resistance and gray mold, also have stronger resistance to other damage by disease and insect, obviously can reduce times for spraying, be conducive to Green fruit production in production.
2, yielding ability is good, every 667m2 more than average yield 5000kg, is significantly higher than current greenhouse by solar heat most high yielding variety Du carat.
3, best in quality.Titrable acidity 0.6%, soluble solid 10.0%, total Soluble Sugar 6.0%.Fruit is large, and one-level sequence fruit mean fruit weight is 45.7g, and maximum single fruit weight is 106.4g, and fruit is sour-sweet moderate, mouthfeel is good, savory, to a certain extent its maternal chapter Ji's of heredity taste and fruit type feature, belong to " hesperidium " class high quality fruit, commodity value is high, and the market competitiveness is strong.
4, storage tolerance.The hardness of fruit is large, is 0.55kg/cm2, and close to male parent Du carat, be significantly better than a maternal chapter Ji, storage property improves greatly, and can find a good sale in all parts of the country, shelf life is long.
5, Cultivation in Greenhouse is applicable to.This kind dormancy is shallow, early ripe, more Zao than Du Kela 20 days, more late than a chapter Ji 10 days.
2005-2009 Project Unit sells nearly 2,000,000 strains of red reality U.S. original seed seedling, can expand numerous 1.2 hundred million strain breeding seedlings, adds that in all parts of the country introducing a fine variety from this Project Unit expands numerous amount about 300,000,000 strain breeding seedling, accumulative more 60,000 mu of production application area.Compare with old kind Japan Feng Xiang, red real U.S. every mu of volume increase 2.5-3 ton, increment 15000 yuan, accumulative volume increase strawberry 14-17 ten thousand tons, increases social benefit 900,000,000 yuan.
Claims (7)
1. a New Strawberry Variety breeding method, is characterized in that:
1. petal female parent being in large flower bud phase flower is pushed aside, pulls out whole stamen,
2. win male parent flower to pollinate to female parent, with paper bag, fresh idea packaged after pollination,
3. when 10-15d holder starts to expand and bears young fruit, paper bag is removed, successively the hybridization fruit of maturation is taken off after 30-40 days, by wash seeds, dry after be put in refrigerator, refrigerate stand-by under 1-3 DEG C of condition;
4. get sandy loam, sow after autoclaving sterilization after crossing 1-2.5mm sieve;
5. after surviving, the young plant Miao Dingzhi that grows directly from seeds possessing 4-5 sheet leaf is carried out field strain Evaluating and screening to green house forcing culture, obtain screening new varieties.
2., by New Strawberry Variety breeding method according to claim 1, it is characterized in that: when pulling out whole stamen, can not column cap be hurt; Put paper bag immediately after pulling out whole stamen until open when pollinating, with paper bag, fresh idea is packaged immediately after pollination.
3. by New Strawberry Variety breeding method according to claim 1, it is characterized in that: pollination is the flower of petal male parent do not launched, collect in clean culture dish, the column cap of maternal emasculation is formerly smeared gently, or direct tweezers are pulled up male parent flower pesticide and are put on the column cap of maternal gynoecium.
4. by New Strawberry Variety breeding method according to claim 1, it is characterized in that: sowing be by sterilizing after soil be dispensed in the middle of flowerpot, seed is evenly play wherein, seed covers the thin soil of one one-tenth 2-3mm, the moisturizing of water spray overlay film, move on to winter in green house and survive the winter, after seedling, move to nursery.
5. by New Strawberry Variety breeding method according to claim 4, it is characterized in that: before sowing, soil soaks in basin, then taking-up control is dry; Water spray is evenly squirted by top layer earthing with sprayer.
6., by New Strawberry Variety breeding method according to claim 1, it is characterized in that: autoclaving described in step 4 is sterilizing 15min under 0.1Mpa temperature 120 DEG C of conditions.
7., by New Strawberry Variety breeding method according to claim 1, it is characterized in that: the most in good time phase of pollination is April, the most in good time phase of sowing is the September then obtaining seed.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938325A (en) * | 2015-06-23 | 2015-09-30 | 安徽省农业科学院园艺研究所 | Strawberry hybridization and seed seedling method |
| CN106973784A (en) * | 2017-03-31 | 2017-07-25 | 平凉锐博知识产权服务有限公司 | A kind of breeding method of New Strawberry Variety |
| CN107182776A (en) * | 2017-06-27 | 2017-09-22 | 河北省农林科学院石家庄果树研究所 | A kind of cross breeding method of strawberry |
| CN111859672A (en) * | 2020-07-22 | 2020-10-30 | 南京工业职业技术大学 | Intelligent construction method for ecological environment of strawberry and bee composite facility |
| CN114793882A (en) * | 2022-04-21 | 2022-07-29 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | Breeding method and application of pink strawberry |
-
2013
- 2013-08-01 CN CN201310331481.XA patent/CN104335891A/en active Pending
Non-Patent Citations (1)
| Title |
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| 江苏省农业科学院园艺研究所等: "《草莓品种与栽培》", 30 June 1993 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938325A (en) * | 2015-06-23 | 2015-09-30 | 安徽省农业科学院园艺研究所 | Strawberry hybridization and seed seedling method |
| CN106973784A (en) * | 2017-03-31 | 2017-07-25 | 平凉锐博知识产权服务有限公司 | A kind of breeding method of New Strawberry Variety |
| CN107182776A (en) * | 2017-06-27 | 2017-09-22 | 河北省农林科学院石家庄果树研究所 | A kind of cross breeding method of strawberry |
| CN111859672A (en) * | 2020-07-22 | 2020-10-30 | 南京工业职业技术大学 | Intelligent construction method for ecological environment of strawberry and bee composite facility |
| CN114793882A (en) * | 2022-04-21 | 2022-07-29 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | Breeding method and application of pink strawberry |
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