CN107182776A - A kind of cross breeding method of strawberry - Google Patents

A kind of cross breeding method of strawberry Download PDF

Info

Publication number
CN107182776A
CN107182776A CN201710500417.8A CN201710500417A CN107182776A CN 107182776 A CN107182776 A CN 107182776A CN 201710500417 A CN201710500417 A CN 201710500417A CN 107182776 A CN107182776 A CN 107182776A
Authority
CN
China
Prior art keywords
seedling
bud
hybridizing
strawberry
breeding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710500417.8A
Other languages
Chinese (zh)
Inventor
张建军
杨雷
李莉
杨莉
董辉
马文会
杨秋叶
范婧芳
李海山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shijiazhuang Institute of Fruit Trees of Hebei Academy of Agriculture and ForestrySciences
Original Assignee
Shijiazhuang Institute of Fruit Trees of Hebei Academy of Agriculture and ForestrySciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shijiazhuang Institute of Fruit Trees of Hebei Academy of Agriculture and ForestrySciences filed Critical Shijiazhuang Institute of Fruit Trees of Hebei Academy of Agriculture and ForestrySciences
Priority to CN201710500417.8A priority Critical patent/CN107182776A/en
Publication of CN107182776A publication Critical patent/CN107182776A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • A01G13/02Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0237Devices for protecting a specific part of a plant, e.g. roots, trunk or fruits
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/027Apparatus for pollination
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Toxicology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention provides a kind of cross breeding method of strawberry, and it is cross-breeding with American-European kind (♀) × Japanese kind (♂) or Japanese kind (♀) × America and Europe's kind (♂), specifically includes following steps:After the pollen of collection male parent, maternal bud emasculation, pollination, seed collection, sowing, the cultivation by hybridizing seedling, the primary election for hybridizing seedling, the final election for hybridizing seedling, the final election of hybridization seedling, final final election goes out the steps such as target strain.The first familiar generation that this method is cultivated is by American-European kind together with the sets of advantages of Japanese kind, and the new varieties selected have the advantages that quality is excellent, yield is high, hardness is big, disease resistance is strong.

Description

A kind of cross breeding method of strawberry
Technical field
The present invention relates to planting technology field, more particularly to a kind of cross breeding method of strawberry.
Background technology
Strawberry belongs to rose family Fragaria herbaceos perennial, and fruit colour is gorgeous, is of high nutritive value, is referred to as in fruit Queen, is well received by consumers.Strawberry occupies critical role in Fruits production, and Strawberry industry turns into China's fruit industry to be sent out One of exhibition most fast industry, more than 2,000,000 mu of current China strawberry area, the t of annual production more than 200 ten thousand, the first in the world, wherein 85% For facility cultivation.Wherein protecting field forcing culture (shallow dormancy kind) Strawberry ripening morning, price are high, from field planting to maturation normally only The time of 3 first quarter moons is needed, the time of fruit maturation typically starts from mid-December, can be largely ripe to New Year's Day and the Spring Festival, Now strawberry is the unique fresh fruit of in the market, and price is unusual in 40 yuan/kilogram to 160 yuan/kilogram, economy effect according to area Benefit is very notable.
The shallow dormancy main breed of the suitable forcing culture of current China mainly includes American-European kind " sweet tea Charlie " and Japanese product Plant " a chapter Ji " and " beauty " etc..The American-European general hardness of kind is big, better resistance, yield are high, and fresh food quality difference is not by Consumption of China Person welcomes;Japanese variety and quality is excellent, but fruit is soft, disease resistance is poor, numerous seedling difficult, seriously constrains the health of China's strawberry industry Development.
The content of the invention
It is an object of the invention to the defect for solving above-mentioned prior art presence, there is provided a kind of quality is excellent, yield is high, hardness Greatly, the cross breeding method of the strong strawberry of disease resistance.
A kind of cross breeding method of strawberry, it is characterised in that with American-European kind (♀) × Japanese kind (♂) or Japanese product Plant (♀) × American-European kind (♂) to be cross-breeding, specifically include following steps:
Step 1:Gather the pollen of male parent;
Step 2:Maternal bud emasculation, pollination;
When being pollinated, pollination contact head uses the solid paper web that newspaper is rolled into, and paper web one end is whittled into cone with knife, The one end cut dips pollen, and contact head gently contacts epiclinal column cap and completes pollination;Then newspaper bag is put, and report Paper bag stitch is real;
Step 3:Seed collection;
Strawberry fruit is picked when fruit is fully ripe and seed is collected with beater, is dried in the shade in room temperature and room temperature is deposited Put 10d;
Step 4:Sowing;
Step 5:Grow directly from seeds by the cultivation for hybridizing seedling, the primary election for hybridizing seedling, the final election for hybridizing seedling, hybridization After the final election of seedling, final final election goes out target strain.
Further, method as described above, including:The pollen of male parent is gathered described in step 1 to be included:From paternal plant Just budding, healthy and strong bud is taken, the petal on bud is peeled off with the pincet of tip, the stamen inside bud Take off, stamen is placed on clean template, make its natural loose powder.
Further, in method as described above, step 2 before being pollinated, including:The selection flower that a bud just ready to burst Flower bud, bud is carefully peeled off with the tweezers of tip, removes the stamen inside bud, selects to be pollinated during calm, fine day 10 to 16.
Further, being sowed in method as described above, step 4 includes:Seed is encased with gauze in 30 DEG C of temperature before sowing 24h is soaked in water, the seed with gauze, which is put into 0 DEG C~7 DEG C refrigerator moisture keepings, afterwards refrigerates 7d, and treated seed point is sowed at In hole tray, 2~4mm of program request depth.
Further, method as described above, the cultivation of the hybridization seedling includes:Cross hybrid seedling in seedlings nursing plate is grown to During with 2~3 true leaves, plant and arrive seedling breeding garden, Routine Management.
Further, method as described above, after final election goes out target strain, in addition to:Regional testing:To what is filtered out Target strain is in different eco-species growing areas, the regional testing of continuous 3 years of progress;It is steady that the strain of regional testing such as shows character Determine, meet breeding objective, then new lines are authorized and named.
Beneficial effect:
The invention provides a kind of cross breeding method of strawberry, the first familiar generation that this method is cultivated is by American-European kind and day Be kind sets of advantages together, the new varieties selected have the advantages that quality is excellent, yield is high, hardness is big, disease resistance is strong; The present invention uses newspaper paper web to have the advantages that to injure pistil stigma small, saving pollen, uniform pollination as pollinating tool;This Invention newspaper bag entangles allocarpy, can guarantee that the flower just pollinated not by the advantage of other pollen contaminations;The present invention uses report Paper bag good permeability, can guarantee that the normal growth and development of allocarpy;The method of present invention beater harvest hybrid seed has Advantage more than efficiency high, harvest seed;The type of seeding of the present invention has the advantages that emergence rate is high, it is neat to emerge.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, below technical scheme in the present invention carry out it is clear Chu, it is fully described by, it is clear that described embodiment is a part of embodiment of the invention, rather than whole embodiments.It is based on Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made Embodiment, belongs to the scope of protection of the invention.
The invention provides a kind of cross breeding method of strawberry, comprise the following steps:
1st, it is cross-breeding with American-European kind (♀) × Japanese kind (♂) or Japanese kind (♀) × America and Europe's kind (♂).
2nd, the pollen of male parent is gathered:Take that a bud just ready to burst from paternal plant, healthy and strong bud, with the pincet of tip The petal on bud is peeled off, carefully the stamen inside bud is taken off, stamen is placed on clean template, makes it Natural loose powder.It is mounted in after pollen dispersed is good stand-by in medical vial.
3rd, female parent selection:Growth selection is healthy and strong, plant that is being endangered without disease pest is used as female parent.
4th, maternal bud emasculation, pollination:The selection bud that a bud just ready to burst, carefully peels off bud with the tweezers of tip, goes Fall the stamen inside bud, select to be pollinated during calm, fine day 10 to 16, the solid paper that pollination contact head is rolled into using newspaper Cylinder, paper web one end is whittled into cone with knife, and the one end cut is put into the medical vial of full blossom powder and dips pollen, contact head Gently contact epiclinal column cap and complete pollination;Then put the newspaper bag pasted, fasten small label, and with nylon rope report Paper bag stitch is real.
5th, seed collection:Strawberry fruit is picked when fruit is fully ripe and seed is collected with beater, in room temperature Dry in the shade and room temperature storage 10d.
6th, sow:Seed being encased with gauze before sowing and 24h being soaked in 30 DEG C of warm water, the seed with gauze is put into afterwards 0 DEG C~7 DEG C refrigerator moisture keepings refrigerate 7d, treated seed point are sowed in hole tray, 2~4mm of program request depth.
7th, the cultivation of seedling is hybridized:When cross hybrid seedling in seedlings nursing plate is grown to 2~3 true leaves, plant and arrive seedling Cultivate garden, Routine Management.
8th, the primary election of seedling is hybridized:The hybridization seedling direct planting cultivated to primary election garden, carried out according to breeding objective The identification of character:Including fruit quality, fruit shapes, fruit color, the hardness of fruit, plant yield, plant disease resistance etc., so that Tentatively select the excellent strain of target.
9th, the final election of seedling is hybridized:The identification of character is carried out according to breeding objective to the excellent strain selected, fruit product are included Matter, fruit shapes, fruit color, the hardness of fruit, plant yield, plant disease resistance etc., so that final election goes out target major clique.
10th, the final election of seedling is hybridized:The major clique gone out to final election continues to carry out contrast screening according to breeding objective, final to determine Select target strain.
11st, regional testing:To the target strain that filters out in different eco-species growing areas, the region examination of continuous 3 years is carried out Test.
12nd, the authorization of new varieties:The strain of regional testing such as shows character stabilization, meets breeding objective, then to new product It is to be authorized and named.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that:It still may be used To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic; And these modification or replace, do not make appropriate technical solution essence depart from various embodiments of the present invention technical scheme spirit and Scope.

Claims (6)

1. a kind of cross breeding method of strawberry, it is characterised in that with American-European kind (♀) × Japanese kind (♂) or Japanese kind (♀) × American-European kind (♂) is cross-breeding, and specifically includes following steps:
Step 1:Gather the pollen of male parent;
Step 2:Maternal bud emasculation, pollination;
When being pollinated, pollination contact head uses the solid paper web that newspaper is rolled into, and paper web one end is whittled into cone with knife, cut One end dip pollen, contact head gently contacts epiclinal column cap and completes pollination;Then newspaper bag is put, and newspaper bag Stitch is real;
Step 3:Seed collection;
Strawberry fruit is picked when fruit is fully ripe and seed is collected with beater, dried in the shade and room temperature storage in room temperature 10d;
Step 4:Sowing;
Step 5:By the cultivation for hybridizing seedling, the primary election for hybridizing seedling, the final election for hybridizing seedling, hybridization seedling After final election, final final election goes out target strain.
2. according to the method described in claim 1, it is characterised in that including:The pollen of male parent is gathered described in step 1 to be included:From Take that a bud just ready to burst on paternal plant, healthy and strong bud, the petal on bud is peeled off with the pincet of tip, bud The stamen of the inside is taken off, and stamen is placed on clean template, makes its natural loose powder.
3. according to the method described in claim 1, it is characterised in that in step 2 before being pollinated, including:Selection is in bud Bud to be put, bud is carefully peeled off with the tweezers of tip, removes the stamen inside bud, when selecting calm, fine day 10 to 16 Pollinated.
4. according to the method described in claim 1, it is characterised in that sowing includes in step 4:Before sowing seed is encased with gauze 24h is soaked in 30 DEG C of warm water, the seed with gauze, which is put into 0 DEG C~7 DEG C refrigerator moisture keepings, afterwards refrigerates 7d, by treated kind Son point is sowed in hole tray, 2~4mm of program request depth.
5. according to the method described in claim 1, it is characterised in that the cultivation of the hybridization seedling includes:In seedlings nursing plate When cross hybrid seedling is grown to 2~3 true leaves, plant and arrive seedling breeding garden, Routine Management.
6. according to the method described in claim 1, it is characterised in that after final election goes out target strain, in addition to:
Regional testing:To the target strain that filters out in different eco-species growing areas, the regional testing of continuous 3 years is carried out;Region The strain of experiment such as shows character stabilization, meets breeding objective, then new lines is authorized and named.
CN201710500417.8A 2017-06-27 2017-06-27 A kind of cross breeding method of strawberry Pending CN107182776A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710500417.8A CN107182776A (en) 2017-06-27 2017-06-27 A kind of cross breeding method of strawberry

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710500417.8A CN107182776A (en) 2017-06-27 2017-06-27 A kind of cross breeding method of strawberry

Publications (1)

Publication Number Publication Date
CN107182776A true CN107182776A (en) 2017-09-22

Family

ID=59881641

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710500417.8A Pending CN107182776A (en) 2017-06-27 2017-06-27 A kind of cross breeding method of strawberry

Country Status (1)

Country Link
CN (1) CN107182776A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111316920A (en) * 2020-04-15 2020-06-23 蚌埠海上明珠农业科技发展有限公司 Fengxiang all-star hybrid strawberry
CN111345238A (en) * 2020-04-15 2020-06-30 蚌埠海上明珠农业科技发展有限公司 Hybrid strawberry of rosette and agaricus blazei

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103814812A (en) * 2014-03-12 2014-05-28 中国热带农业科学院热带作物品种资源研究所 Method for cross-breeding of guava
CN104081912A (en) * 2014-07-21 2014-10-08 甘肃省治沙研究所 Method for seed germination and seedling growing on wild lycium ruthenicum
CN104335891A (en) * 2013-08-01 2015-02-11 东港市草莓研究所 A cultivation method of new strawberry species
CN104472344A (en) * 2014-12-23 2015-04-01 江苏省农业科学院 Breeding method of large fruit type day-neutralstrawberry
CN104885899A (en) * 2014-03-05 2015-09-09 刘亚新 New high-yield and high-quality strawberry variety, breeding method, cultivation method and applications thereof
CN104938325A (en) * 2015-06-23 2015-09-30 安徽省农业科学院园艺研究所 Strawberry hybridization and seed seedling method
CN105265304A (en) * 2014-07-30 2016-01-27 河北省农林科学院昌黎果树研究所 Chinese chestnut cross pollination method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104335891A (en) * 2013-08-01 2015-02-11 东港市草莓研究所 A cultivation method of new strawberry species
CN104885899A (en) * 2014-03-05 2015-09-09 刘亚新 New high-yield and high-quality strawberry variety, breeding method, cultivation method and applications thereof
CN103814812A (en) * 2014-03-12 2014-05-28 中国热带农业科学院热带作物品种资源研究所 Method for cross-breeding of guava
CN104081912A (en) * 2014-07-21 2014-10-08 甘肃省治沙研究所 Method for seed germination and seedling growing on wild lycium ruthenicum
CN105265304A (en) * 2014-07-30 2016-01-27 河北省农林科学院昌黎果树研究所 Chinese chestnut cross pollination method
CN104472344A (en) * 2014-12-23 2015-04-01 江苏省农业科学院 Breeding method of large fruit type day-neutralstrawberry
CN104938325A (en) * 2015-06-23 2015-09-30 安徽省农业科学院园艺研究所 Strawberry hybridization and seed seedling method

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
北京农业大学等: "《作物栽培学(下册)》", 31 May 1962, 农业出版社 *
吕中伟等: "《葡萄高产栽培与果园管理》", 30 June 2015, 陕西新华出版传媒集团 陕西科学技术出版社 *
朱立新等: "《草莓园艺工培训教材》", 30 June 2008, 金盾出版社 *
王桂霞等: "草莓新品种——‘冬香’的选育", 《果农之友》 *
范双喜等: "《园艺植物栽培学》", 31 August 2007, 中国农业大学出版社 *
赵锦彪等: "《桃生产配套技术手册》", 28 February 2013, 中国农业出版社 *
雷世俊等: "《草莓高效栽培》", 31 December 2015, 山东科学技术出版社 *
龙兴桂: "《苹果栽培管理实用技术大全》", 31 December 1993, 农业出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111316920A (en) * 2020-04-15 2020-06-23 蚌埠海上明珠农业科技发展有限公司 Fengxiang all-star hybrid strawberry
CN111345238A (en) * 2020-04-15 2020-06-30 蚌埠海上明珠农业科技发展有限公司 Hybrid strawberry of rosette and agaricus blazei

Similar Documents

Publication Publication Date Title
CN103975849B (en) A kind of precocity, the breeding of disease-resistant, the pale reddish brown Broccoli of loose type and breeding method
US6355865B1 (en) Pollenizer plants for use in the production of seedless watermelon
CN102524053B (en) Seed production method for hybrid pepper variety
CN101496494B (en) Crossbreeding method for variety of luffa
CN101438675B (en) Method for breeding hybrid variety of Camellia oleifera
CN103975848B (en) Breeding and cultivating method for early-matured and disease-resistant brassica oleracea
JP2006507796A (en) A tetraploid watermelon that produces small fruits
CN103348910A (en) Method for transforming sterile line of Chinese kale by utilizing vernalization and generation adding
CN103181291A (en) Cultivation and selection method of red sage seeds with high germination rate
CN103168681B (en) Breeding method of early-ripe and disease-resistant broccolini
CN107182776A (en) A kind of cross breeding method of strawberry
CN103988775A (en) Method for breeding and cultivating early ripening disease-resistant stem broccoli
US20190200553A1 (en) Method for Producing Rice Haploid by Rice X Maize Hybridization
CN101720664A (en) Cultivation method of oranges
CN106106122B (en) Leaf produces the breeding method of astaxanthin tomato variety
CN115039688A (en) Oat crossbreeding method based on potted plant planting
CN111642388B (en) Creating method of excellent germplasm of red flower ginkgo strawberry
CN111011206A (en) Method for cultivating new pepper variety by distant hybridization grafting
CN105075852A (en) Method for hybridizing and breeding water dropwort
CN111742840A (en) Method for mutation breeding of tetraploid of black vegetable
CN106417005B (en) Water chestnut artificial hybridization breeding method
CN106879457B (en) A kind of selection of high yielding soybeans kind
CN106106121B (en) A kind of breeding method of leaf production astaxanthin tomato variety
CN103155849A (en) Breeding method of selenium-enriched cocozelle variety
CN107295967A (en) A kind of gumbo combined hybrid method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170922

RJ01 Rejection of invention patent application after publication