CN106106122B - Leaf produces the breeding method of astaxanthin tomato variety - Google Patents
Leaf produces the breeding method of astaxanthin tomato variety Download PDFInfo
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- CN106106122B CN106106122B CN201610003289.1A CN201610003289A CN106106122B CN 106106122 B CN106106122 B CN 106106122B CN 201610003289 A CN201610003289 A CN 201610003289A CN 106106122 B CN106106122 B CN 106106122B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The breeding method of New Tomato Variety ' cloud shrimp No. 2 ', it is female parent with the old kind of Yunnan local ' big flat-top ', production astaxanthin engineering tomato ' red No. 1 of shrimp ' is that male parent is hybridized, and its content is detected with acetone extraction blade astaxanthin, and using UPLC methods.' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high-yield astaxanthin.It is mainly characterized by, plant determinate growth, plant height 130cm or so, kermesinus, robust growth；For stem yellow green to light brown, branch ability is strong；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reach 2.27mg/g.Calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit large red, fruit shoulder aubergine, circular to oblateness, fruit shape index 0.67~0.79, fruit surface smooth, gloss is bright, and pulp is thick, pink colour, and placenta jelly is red, and ventricle 5~7, hardness is moderate, and mean fruit weight 150g, maximum single fruit weight is up to 350g.
Description
Technical field：
The invention belongs to biological technical field, and in particular to the breeding method of tomato plants Hybrid, more specifically, relating to
And with ' big flat-top ' be female parent, it is good to obtain a kind of fruit quality to produce astaxanthin engineering tomato ' red No. 1 of shrimp ' as paternal hybrid, and
The breeding method of the seed of single cross of blade high-yield astaxanthin.
Background technology：
' big flat-top ' is the local old kind that Yunnan Province's Chuxiong Prefecture Yuanmou County is planted earliest, and its plant is determinate growth,
Plant height 120cm or so, growing way is strong, and branch is very competent, and the leaf of stem 8~10 raw 1st inflorescence, belongs to middle-late ripening variety；Adapt to
Property strong, heat-resisting, easily cultivation, fruit-setting rate is high.Fruit type is neat, and oblate, fruit face colouring is uniform, and smooth no rib, gloss is bright, mellow fruit
Real large red, pulp is thick, and ventricle 5~7, hardness is moderate.Mean fruit weight 150g, maximum single fruit weight is up to 350g, in Kunming
Regional spring greenhouse cultivation, it is 160 days or so from harvesting is seeded into.Produce astaxanthin engineering tomato ' red No. 1 of shrimp ' be its plant,
Flower, leaf, fruit are morphologically with Lycopersicon esculentum indifference, the difference mainly in color.Specifically, plant determinate growth, strain
High 110cm or so.The erect type of stem half, the thick 2.6~4.7cm of stem, 4~6 branches, yellow green is to light brown.Common blade profile, petiole
Green is to kermesinus, blade kermesinus.Hermaphrodite flower, calyx kermesinus, radial corolla are orange-yellow orange extremely to Chinese red, flower pesticide
Red, column cap and harbors young fruit brownish red.Fruit is subsphaeroidal, Chinese red, mean fruit weight 100g or so after maturation, its fruit shrimp
The average value of blue or green cellulose content is 1.63mg/g.
It is female parent with the old kind of Yunnan local ' big flat-top ', to produce astaxanthin engineering tomato ' red No. 1 of shrimp ' as paternal hybrid
It is cross-breeding, so as to obtain the breeding method of all excellent New Tomato Varieties of a seed pod leaf quality, there is not been reported with technology.
The content of the invention：
By maternal, production astaxanthin engineering tomato of common cultivation tomato it is that male parent is entered it is an object of the invention to provide one kind
Row crossbreeding, obtains that a kind of fruit quality is good, and the breeding method of the New Tomato Variety of blade high-yield astaxanthin.
The above-mentioned purpose of the present invention is realized by following technical scheme：
The breeding method of tomato variety ' cloud shrimp No. 2 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting,
Seed treatment, content astaxanthin determination step, it is female parent with ' big flat-top ', production astaxanthin engineering tomato ' red No. 1 of shrimp ' is male parent
Hybridized, its content is detected with acetone extraction blade astaxanthin, and using UPLC methods.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein described pollen collection is, male parent is more early than maternal
Sowing 20 days, the full-bloom stage morning, collection male parent ' red No. 1 of shrimp ' petal deploys 170-190 ° and pattern is partially red after dew is dry
Flower, chooses stamen, is spread out on blank sheet of paper, indicates kind name and collection date, is placed in clean place and dries in the shade naturally, treats that flower pesticide splits
Open, when pollen sheds, it is standby to collect pollen；Described maternal emasculation is outer in pollination the previous day selection ' big flat-top ' corolla elongation
Dew, color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, the bud bloomed in next day
Carry out emasculation；Described artificial pollination is 20~25 DEG C of fine day morning 8-9 points, temperature pollinations after daily maternal field dew is dry；
Described fruit harvesting is 50~60 days harvesting ripening fruits after pollination；Described seed treatment is that complete red ripe fruit is adopted
After receipts, treat that after-ripening takes kind in 1~2 day again；Described content astaxanthin measure is to use acetone extraction blade astaxanthin, the detection of UPLC methods
Its content.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein collected in described pollen collection step
Pollen is temporarily stored is stored in -80 DEG C of refrigerators with silica gel with 4 DEG C of refrigerator cold-storages, long-term storage as drier.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein described maternal emasculation step is steady with left hand
Bud base portion is held, the right hand clamps flower pesticide with tweezers and all extracts its stamen, or is together peeled off together with petal, notices that emasculation will be done
Only, isolation is paid attention to after emasculation, while rejects the flower for being not suitable for pollination and selfing fruit.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein described artificial pollination step is optimum flat
Equal temperature is 20~25 DEG C, dips a small amount of pollen point with the rubber tip of common pencil and invests on the column cap of maternal emasculation flower, or
Pollen is directly placed on blank sheet of paper by person, is directly contacted the column cap of emasculation, is dipped a small amount of pollen.It is bent with 6 × 8cm tea bag bag baggings
Safety pin is fixed, listing mark, and Parent combination number and pollination date are indicated on plate, bag taking after pollinating 10 days, is observed simultaneously
Bear fruit situation, in time training cleaning, remove the bud do not pollinated and the inflorescence of new life, training are pruned, extract unnecessary axillary bud
And the branch of excessive growth, carry out liquid manure coordinated management and the prevention and control of plant diseases, pest control.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein only harvesting life in described fruit harvesting step
Long normotrophic fruit, does not harvest unmarked fallen fruits and malformed fruit.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein horizontal with knife in described seed treatment step
Splitted to by fruit, seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, be white to fermentate surface, use
Wooden stick stirs, and seed sinks, and seed is pinched with hand, and pectin separates with seed, without seed is cleaned when sticking sliding sense, removes pulp, debris
With flat seed, load in double gauze bag and squeeze the water out, be spread out on the overhead canvas in bottom or blotting paper and dry in the shade, stir several times, make
The moisture of the surface of the seed evaporates rapidly, until when seed moisture content is down to 7%~8%, paper bag packing, room temperature storage, next year 3
Moon sowing.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein the determination step of described astaxanthin is to wait to plant
Strain is grown up, and the lower 4-5 floor blades in collection New Tomato Variety ' cloud shrimp No. 2 ' top, freeze-dried acquisition blade dry powder, weighs sample
50mg, adds acetone extraction 4 times, until extract is colourless, collects extract and is settled to 5ml, loading determines pigment after suction filtration, sets
3 repetitions, the content of its astaxanthin is determined using ultra-performance liquid chromatography.
A kind of breeding method of tomato variety ' cloud shrimp No. 2 ' of the present invention, it comprises the following steps：(1) pollen collection, father
This was than maternal planting early enough 20 days, and the full-bloom stage morning, collection male parent ' red No. 1 of shrimp ' petal deployed 170-190 ° and flower after dew is dry
The red flower of colour cast, choose stamen in indoor tweezers, spread out on thin and flexible blank sheet of paper, indicate kind name and collection day
Phase, it is placed in clean place and dries in the shade naturally, treat Anther dehiscence, when pollen sheds, collection pollen is standby, and pollen is stored temporarily in 4
DEG C refrigerator, long-term storage are stored in -80 DEG C of refrigerators with silica gel as drier；(2) maternal emasculation, pollination the previous day selection are ' big
Flat-top ' corolla elongation is exposed, and color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture,
The bud bloomed in next day carries out emasculation, and during emasculation, left hand surely holds bud base portion, and the right hand with tweezers clamps flower pesticide, and its stamen is complete
Portion is extractd, or is together peeled off together with petal, is isolated after emasculation, while rejects the flower for being not suitable for pollination and selfing fruit；(3) it is artificial
Pollination, starting to pollinate after maternal field dew is dry, fine day is carried out in morning 8-9 points, and suitable temperature on average of pollinating is 20~25 DEG C,
A small amount of pollen is dipped with the rubber tip of common pencil, point is invested on the column cap of maternal emasculation flower or pollen is placed in into blank sheet of paper
On, the column cap of emasculation is directly contacted, dips a small amount of pollen, with 6 × 8cm tea bag bag baggings, paper clip is fixed, listing mark, board
Parent combination number and pollination date are indicated on son, bag taking after pollinating 10 days, while situation of bearing fruit is observed, training is clear in time
Reason, removes the bud do not pollinated and the inflorescence of new life, training are pruned, and extracts unnecessary axillary bud and the branch of excessive growth, carries out liquid manure association
With management and the preventing and treating of pest and disease damage；(4) fruit harvesting, 50~60 days harvesting ripening fruits after pollination, only harvesting are grown just
Normal fruit, unmarked fallen fruits and malformed fruit are not harvested；(5) after seed treatment, completely red ripe fruit harvesting, after-ripening 1
Kind is taken again within~2 days, laterally fruit is splitted with knife, seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day,
Until fermentate superficial white, is stirred with wooden stick, seed sinks, and seed is pinched with hand, and pectin separates with seed, feels without glutinous slide
Seed is cleaned, is rinsed with clear water, removes pulp, debris and flat seed, loads in double gauze bag and squeezes the water out, be spread out in bottom and make somebody a mere figurehead
Canvas or blotting paper on, stir, the moisture of the surface of the seed is evaporated rapidly, until seed moisture content is down to 7%~8%
When, paper bag packing, room temperature storage, sowing in March in next year；(6) content astaxanthin determines, and New Tomato Variety ' cloud shrimp No. 2 ' is after planting
Plant to be planted is grown up, and the lower 4-5 layer blades in harvesting top, freeze-dried acquisition blade dry powder, is weighed sample 50mg, is added acetone extraction 4
It is secondary, until extract is colourless, collects extract and be settled to 5ml, loading determines pigment after suction filtration, 3 repetitions is set, using superelevation
Effect liquid phase chromatogram UPLC methods determine the content of its astaxanthin.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, by ' big flat-top ' it is female parent wherein described, production shrimp is blue or green
Plain engineering tomato ' red No. 1 of shrimp ' is that the biological characteristics of ' cloud shrimp No. 2 ' that male parent is hybridized to obtain is：' cloud shrimp No. 2 ' combines
The main merit of Parent, fruit quality is good, and blade high-yield astaxanthin, it is mainly characterized by：Plant determinate growth
Type, plant height 130cm or so, kermesinus, robust growth；For stem yellow green to light brown, branch is very competent；Common blade profile, petiole
Yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.27mg/g, and calyx kermesinus is radial
Corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red, fruit large red, fruit shoulder aubergine,
Circle is to oblateness, and fruit shape index 0.67~0.79, fruit surface smooth, gloss is bright, and pulp is thick, pink colour, and placenta jelly is red, the heart
Room 5~7, hardness is moderate, and mean fruit weight 150g, maximum single fruit weight reaches 350g.
Compared with prior art, excellent benefit of the invention is, the tomato variety cultivated by breeding method of the present invention
' cloud shrimp No. 2 ' reaches 2mg/g as objective trait using dry matter weight of leaf astaxanthin average content, fruit properties is taken into account, with plant color
Whether it is mark property of the kermesinus as discriminating Hybrid and ' big flat-top '.With reference to Phenotypic Observation and blade content astaxanthin
Measure assess, ' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high-yield astaxanthin.Its
It is mainly characterized by, plant determinate growth, plant height 130cm or so, kermesinus, robust growth；Stem yellow green is to light brown, branch
It is very competent；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reach
2.27mg/g.Calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit palm fibre
It is red.Fruit large red, fruit shoulder aubergine, circle is to oblateness, and fruit shape index 0.67~0.79, fruit surface smooth, gloss is bright, fruit
Meat is thick, pink colour, and placenta jelly is red, and ventricle 5~7, hardness is moderate, mean fruit weight 150g, and maximum single fruit weight is reachable
350g。
Embodiment：
The essentiality content of the present invention is further illustrated with the concrete instance of the present invention below, but is not limited with this
The fixed present invention.
Embodiment 1：
The hybridizing method of tomato variety ' cloud shrimp No. 2 '：
It is female parent with the old kind of Yunnan local ' big flat-top ', to produce astaxanthin engineering tomato ' red No. 1 of shrimp ' as paternal hybrid
And obtain.
Maternal ' big flat-top ' tomato is the local old kind that Yunnan Province's Chuxiong Prefecture Yuanmou County is planted earliest, and fruit surface smooth is individual
Head is big, and fruit quality is good, the elite plant strain that material to be tested is reserved seed for planting certainly from gardens College of Horticulture of Yunnan Prov Agriculture University.Male parent
' red No. 1 of shrimp ' is production astaxanthin engineering tomato, cultivated and forms in 2009, is that (Huang JC etc., 2013) such as yellow pretty tides passes through
Gene engineering method, which is screened and is separated to from green alga, can be catalyzed beta carotene and luteole synthesizing astaxanthin in plant cell
Assimilation enzyme and '-hydroxylase gene be transferred in wild-type tomatoes plant (cv.UC82B), obtained transgene tomato is (in 2014
On May 14, is in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, preserving number：CGMCC No
No. 9223, Classification And Nomenclature：' red No. 1 of shrimp ' transgene tomato).2010 to 2012 respectively in bioscience institute of Hong Kong University and
Kunming Inst. of Botany, Chinese Academy of Sciences has carried out the plantation of 4 generations by potted plant mode, and character is stable.In March, 2013 starts
Sowing, ' red No. 1 of shrimp ' than ' big flat-top ' early sowing 20 days, May, full-bloom stage was pollinated the previous day, and the elongation of ' big flat-top ' corolla is exposed,
Color from green to yellow, do not open or crack, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, the bud bloomed in next day are gone by petal
It is male clean, pay attention to isolation.When morning dew is done, collection male parent ' red No. 1 of shrimp ' petal deploys 170~190 ° and pattern is partially red
Flower, chooses stamen in indoor tweezers, spreads out and dry in the air on thin and flexible blank sheet of paper, indicates the collection date, is placed in clean ground
Fang Ziran dries in the shade.Anther dehiscence is treated, when pollen sheds, it is standby to collect pollen.Second day, after maternal field dew is dry, fine day was upper
Noon 8-9 points are pollinated.Specifically, dip the column cap that a small amount of pollen point invests maternal emasculation flower with the rubber tip of common pencil
Above or by pollen it is placed on blank sheet of paper, contacts the column cap of emasculation, directly dip a small amount of pollen.It is bent with 6 × 8cm tea bag bag baggings
Safety pin is fixed, listing mark, and Parent combination number and pollination date are indicated on plate.Bag taking after pollinating 10 days, is observed simultaneously
Bear fruit situation, in time training cleaning, remove the bud do not pollinated and the inflorescence of new life, training are pruned, extract unnecessary axillary bud
And the branch of excessive growth, during which pay attention to liquid manure coordinated management and the preventing and treating of pest and disease damage.Ripening fruits can be harvested within 50~60 days after pollination.No
Unmarked fallen fruits and malformed fruit are harvested, only harvest the normal fruit that grows.After complete red ripe fruit harvesting, can after
Kind is taken again within ripe 1~2 day.When taking kind, laterally fruit is splitted with knife, seed and glue flesh are then squeezed into anhydrous nonmetallic vessel
Interior fermentation, fermentation clean seed after 1~2 day, and the place for being placed in divulging information, drying is filtered dry moisture and dried immediately, until seed
Water content is stored when being down to 7%~8%, sowing in March in next year.Treat that the plant of tomato variety ' cloud shrimp No. 2 ' after planting grows up, in good time
The lower 4-5 layer blades in its top are harvested, freeze-drying obtains dry powder.Sample 50mg accurately is weighed, adds acetone extraction 4 times, until extracting
Liquid is colourless, collects extract and is settled to 5ml, loading determines pigment after suction filtration, 3 repetitions is set, using ultra performance liquid chromatography
(UPLC) method determines the content of its astaxanthin.By 4 plantations of 2014 and 2015, Phenotypic Observation and blade astaxanthin contained
The measurement result of amount shows that ' cloud shrimp No. 2 ' character is excellent neat, and fruit quality is good, and blade content astaxanthin is high, and disease resistance
By force.
The character for the hybrid strain that hybridizing method of the present invention is related to is as follows：
' big flat-top '：The local old kind that Yunnan Province's Chuxiong Prefecture Yuanmou County is planted earliest, its plant are determinate growth, strain
High 120cm or so, growing way is strong, and the leaf of stem 8~10 raw 1st inflorescence, belongs to middle-late ripening variety；Strong adaptability, it is heat-resisting, easily plant
Training, fruit-setting rate are high.Fruit type is neat, and oblate, fruit face colouring is uniform, and smooth no rib, gloss is bright, ripening fruits large red, pulp
Thickness, ventricle 5~7, hardness is moderate.Mean fruit weight 150g, maximum single fruit weight is up to 350g, and in Kunming Areas, spring greenhouse is planted
Training, it is 160 days or so from harvesting is seeded into.
Produce astaxanthin engineering tomato ' red No. 1 of shrimp '：Its plant, flower, leaf, fruit are main morphologically with Lycopersicon esculentum indifference
If the difference in color.Specifically, plant determinate growth, plant height 110cm or so.The erect type of stem half, stem thick 2.6~
4.7cm, 4~6 branches, yellow green is to light brown.Common blade profile, petiole green to kermesinus, blade kermesinus.Hermaphrodite flower,
Calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit
Subsphaeroidal, Chinese red, mean fruit weight 100g or so after maturation, the average value of its fruit content astaxanthin is 1.63mg/g.
New Tomato Variety ' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high yield shrimp
Blue or green element.It is mainly characterized by, plant determinate growth, plant height 130cm or so, kermesinus, robust growth；Stem yellow green is to shallow brown
Color, branch are very competent；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content
Up to 2.27mg/g.Calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit
Brownish red.Fruit large red, fruit shoulder aubergine, circle to oblateness, fruit shape index 0.67~0.79, fruit surface smooth, gloss is bright,
Pulp is thick, pink colour, and placenta jelly is red, and ventricle 5~7, hardness is moderate, mean fruit weight 150g, and maximum single fruit weight is reachable
350g。
Claims (9)
1. the breeding method of tomato variety ' cloud shrimp No. 2 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting, kind
Subprocessing, content astaxanthin determination step, are female parent with ' big flat-top ', and production astaxanthin engineering tomato ' red No. 1 of shrimp ' enters for male parent
' cloud shrimp No. 2 ' is cultivated in row hybridization, detects its content with acetone extraction blade astaxanthin, and using UPLC methods, described pollen is adopted
Collection is, male parent than maternal planting early enough 20 days, the full-bloom stage morning after dew is dry, collection male parent ' red No. 1 of shrimp ' petal expansion 170-
190 ° and the partially red flower of pattern, choose stamen, are spread out on blank sheet of paper, indicate kind name and collection date, are placed in clean place
Naturally dry in the shade, treat Anther dehiscence, when pollen sheds, it is standby to collect pollen；Described maternal emasculation is in pollination the previous day selection
The elongation of ' big flat-top ' corolla is exposed, and color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the flower that sheds that do not ftracture
Powder, the bud bloomed in next day carry out emasculation；Described artificial pollination is fine day morning 8-9 points, temperature after maternal field dew is done
20~25 DEG C of pollinations；Described fruit harvesting is 50~60 days harvesting ripening fruits after pollination；Described seed treatment has been
After complete red ripe fruit harvesting, treat after-ripening 1-2 days and take kind again；Described content astaxanthin measure is blue or green with acetone extraction blade shrimp
Element, UPLC methods detect its content.
2. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that：Described pollen collection
The pollen collected in step is temporarily stored is stored in -80 DEG C of ice with silica gel with 4 DEG C of refrigerator cold-storages, long-term storage as drier
Case.
3. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that：Described maternal emasculation
Step is that bud base portion is surely held with left hand, and the right hand clamps flower pesticide with tweezers and all extracts its stamen, or is together shelled together with petal
Fall, emasculation is clean, isolates after emasculation, while rejects the flower for being not suitable for pollination and selfing fruit.
4. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that：Described artificial pollination
Step temperature is 20~25 DEG C, dips a small amount of pollen point with the rubber tip of common pencil and invests on the column cap of maternal emasculation flower,
Or directly pollen is placed on blank sheet of paper, the column cap of emasculation is directly contacted, dips a small amount of pollen, with 6 × 8cm tea bag bag baggings,
Paper clip is fixed, listing mark, and Parent combination number and pollination date are indicated on plate, bag taking after pollinating 10 days, is seen simultaneously
The situation of bearing fruit is examined, in time training cleaning, remove the bud do not pollinated and the inflorescence of new life, training are pruned, extract unnecessary armpit
Bud and the branch of excessive growth, carry out liquid manure coordinated management and the prevention and control of plant diseases, pest control.
5. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that：Described fruit harvesting
The normal fruit that grows only is harvested in step, does not harvest unmarked fallen fruits and malformed fruit.
6. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that：Described seed treatment
Laterally fruit is splitted with knife in step, seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, to fermentation
Thing surface is white, is stirred with wooden stick, and seed sinks, and seed is pinched with hand, and pectin separates with seed, without cleaning kind when sticking sliding sense
Son, pulp, debris and flat seed are removed, load in double gauze bag and squeeze the water out, be spread out on the overhead canvas in bottom or blotting paper
Dry in the shade, stirring several times, makes the moisture of the surface of the seed evaporate rapidly, until when seed moisture content is down to 7%~8%, paper bag bag
Dress, room temperature storage, sowing in March in next year.
7. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that：Described astaxanthin contains
It is that plant to be planted is grown up to measure determination step, the lower 4-5 floor blades in collection New Tomato Variety ' cloud shrimp No. 2 ' top, freeze-dried acquisition leaf
Piece dry powder, weigh sample 50mg, add acetone extraction 4 times, until extract is colourless, collects extract and be settled to 5ml, after suction filtration on
Sample determines pigment, sets 3 repetitions, and the content of its astaxanthin is determined using ultra-performance liquid chromatography.
8. the breeding method of tomato variety ' cloud shrimp No. 2 ', it is characterised in that this method is female parent with ' big flat-top ', produces astaxanthin work
Journey tomato ' red No. 1 of shrimp ' is that male parent carries out hybridization cultivation ' cloud shrimp No. 2 ', is comprised the following steps：(1) pollen collection, male parent is than female
This planting early enough 20 days, the full-bloom stage morning, collection male parent ' red No. 1 of shrimp ' petal deploys 170-190 ° and pattern is partially red after dew is dry
Flower, choose stamen in indoor tweezers, spread out on thin and flexible blank sheet of paper, indicate kind name and collection the date, be placed in
Clean place is dried in the shade naturally, treats Anther dehiscence, and when pollen sheds, collection pollen is standby, and pollen is stored temporarily in 4 DEG C of refrigerators,
Long-term storage is stored in -80 DEG C of refrigerators with silica gel as drier；(2) maternal emasculation, pollination the previous day selection ' big flat-top ' flower
Hat elongation is exposed, and color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, is opened in next day
Colored bud carries out emasculation, and during emasculation, left hand surely holds bud base portion, and the right hand clamps flower pesticide with tweezers and all extracts its stamen,
Or together peeled off together with petal, isolate after emasculation, while reject the flower for being not suitable for pollination and selfing fruit；(3) artificial pollination, it is female
Start to pollinate after Honda dew is dry, fine day is carried out in morning 8-9 points, and suitable temperature on average of pollinating is 20~25 DEG C, with common
The rubber tip of pencil dips a small amount of pollen, and point is invested on the column cap of maternal emasculation flower or pollen is placed on blank sheet of paper, directly
The column cap of emasculation is contacted, dips a small amount of pollen, with 6 × 8cm tea bag bag baggings, paper clip is fixed, listing mark, is indicated on plate
Parent combination number and pollination date, bag taking after pollinating 10 days, while situation of bearing fruit is observed, training cleaning in time, removal is not
The bud of pollination and the inflorescence of new life, training are pruned, and are extractd unnecessary axillary bud and the branch of excessive growth, are carried out liquid manure coordinated management and disease
The preventing and treating of insect pest；(4) fruit harvesting, 50~60 days harvesting ripening fruits, only harvest the normal fruit that grows, no after pollination
Harvest unmarked fallen fruits and malformed fruit；(5) after seed treatment, completely red ripe fruit harvesting, after-ripening takes kind in 1~2 day again,
Laterally fruit is splitted with knife, seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, until fermentate table
Colourless color, is stirred with wooden stick, and seed sinks, and seed is pinched with hand, and pectin separates with seed, is seed capable of washing without glutinous sliding sense, is used
Clear water rinses, and removes pulp, debris and flat seed, loads in double gauze bag and squeezes the water out, is spread out in the overhead canvas in bottom or suction
On water paper, stir, the moisture of the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%~8%, paper bag packing,
Room temperature storage, sowing in March in next year；(6) content astaxanthin determines, and New Tomato Variety ' cloud shrimp No. 2 ' after planting grow up by plant to be planted, adopts
The lower 4-5 layer blades in top are received, freeze-dried acquisition blade dry powder, sample 50mg is weighed, adds acetone extraction 4 times, until extract
It is colourless, collect extract and be settled to 5ml, loading determines pigment after suction filtration, 3 repetitions is set, using ultra performance liquid chromatography
UPLC methods determine the content of its astaxanthin.
9. the breeding method of the tomato variety ' cloud shrimp No. 2 ' as described in claim 1 or 8, it is characterised in that described by ' big
Flat-top ' to be maternal, the biological characteristics of ' cloud shrimp No. 2 ' that production astaxanthin engineering tomato ' red No. 1 of shrimp ' is hybridized to obtain for male parent
Property is：' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high-yield astaxanthin, and it is main special
Levy and be：Plant determinate growth, plant height 170-190cm, kermesinus, robust growth；Stem yellow green is to light brown, branch ability pole
By force；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reach 2.27mg/g, flower
Calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red, and fruit is big
Red, fruit shoulder aubergine, circle to oblateness, fruit shape index 0.67~0.79, fruit surface smooth, gloss is bright, and pulp is thick, pink colour,
Placenta jelly is red, and ventricle 5~7, hardness is moderate, and mean fruit weight 150g, maximum single fruit weight reaches 350g.
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| CN104206139A (en) * | 2014-08-15 | 2014-12-17 | 中国科学院昆明植物研究所 | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato |
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| CN104206139A (en) * | 2014-08-15 | 2014-12-17 | 中国科学院昆明植物研究所 | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato |
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