CN106106122A - Leaf produces the breeding method of astaxanthin tomato variety - Google Patents
Leaf produces the breeding method of astaxanthin tomato variety Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The breeding method of New Tomato Variety ' cloud shrimp No. 2 ', with the old kind of Yunnan local ' big flat-top ' for maternal, producing astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' is that male parent hybridizes, and with acetone extraction blade astaxanthin, and uses UPLC method to detect its content.' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high-yield astaxanthin.It is mainly characterized by, plant determinate growth, plant height about 130cm, kermesinus, robust growth；Stem yellow green is to light brown, and branch ability is strong；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.27mg/g.Calyx kermesinus, radial corolla is orange-yellow, and to Chinese red, flower pesticide is orange to red, stigma and harbor young fruit brownish red.Fruit large red, fruit shoulder aubergine, circular to oblate, fruit shape index 0.67～0.79, fruit surface smooth, gloss is bright, and sarcocarp is thick, pink colour, and placenta jelly is red, ventricle 5～7, and hardness is moderate, mean fruit weight 150g, and maximum single fruit weight is up to 350g.
Description
Technical field:
The invention belongs to biological technical field, be specifically related to the breeding method of tomato plants Hybrid, more specifically, relate to
And with ' big flat-top ' for maternal, to produce astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ', to be that paternal hybrid obtains a kind of fruit quality good, and
The breeding method of the seed of single cross of blade high-yield astaxanthin.
Background technology:
' big flat-top ' is the local old kind that Yunnan Province's Chuxiong Prefecture Yuanmou County is planted the earliest, and its plant is determinate growth,
Plant height about 120cm, growing way is strong, and branch is very competent, and stem 8～10 leaf raw 1st inflorescence, belongs to middle-late ripening variety；Adapt to
Property strong, heat-resisting, easily cultivate, fruit-setting rate is high.Fruit type is neat, oblate, and really face colouring is uniform, and smooth without rib, gloss is bright, mellow fruit
Real large red, sarcocarp is thick, and ventricle 5～7, hardness is moderate.Mean fruit weight 150g, maximum single fruit weight is up to 350g, in Kunming
Area greenhouse cultivation in spring, from being seeded into, to gather be about 160 days.Produce astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' be its plant,
Flower, leaf, fruit the most morphologically with the difference in Lycopersicon esculentum indifference, mainly color.Specifically, plant determinate growth, strain
High about 110cm.Stem half erect type, stem thick 2.6～4.7cm, 4～6 branches, yellow green is to light brown.Common blade profile, petiole
Green to kermesinus, blade kermesinus.Hermaphrodite flower, calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange extremely
Redness, stigma and harbor young fruit brownish red.Fruit is subsphaeroidal, Chinese red after maturation, mean fruit weight about 100g, its fruit shrimp
The meansigma methods of blue or green cellulose content is 1.63mg/g.
With the old kind of Yunnan local ' big flat-top ' for maternal, to produce astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' as paternal hybrid
It is cross-breeding, thus there is not been reported with technology to obtain the breeding method of the most excellent New Tomato Variety of a kind of fruit leaf quality.
Summary of the invention:
It is an object of the invention to provide a kind of with common cultivation Fructus Lycopersici esculenti be maternal, produce astaxanthin engineering Fructus Lycopersici esculenti and enter for male parent
Row cross-breeding, it is thus achieved that a kind of fruit quality is good, and the breeding method of the New Tomato Variety of blade high-yield astaxanthin.
The above-mentioned purpose of the present invention is realized by techniques below scheme:
The breeding method of tomato variety ' cloud shrimp No. 2 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting,
Seed treatment, content astaxanthin determination step, with ' big flat-top ' for maternal, producing astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' is male parent
Hybridize, with acetone extraction blade astaxanthin, and use UPLC method to detect its content.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein said pollen collection is, male parent is more Zao than maternal
Sowing 20 days, full-bloom stage gathers male parent ' red No. 1 of shrimp ' petal expansion 170-190 ° and pattern is the reddest after dew is dry the morning
Flower, chooses stamen, spreads out on blank sheet of paper, indicates kind name and gathers the date, is placed in clean place and naturally dries in the shade, treats that flower pesticide splits
Open, when pollen sheds, collect pollen standby；Described maternal emasculation is to select outside the elongation of ' big flat-top ' corolla the previous day in pollination
Dew, color from green to yellow, do not open or crack by petal, and flower pesticide is yellow green, but the pollen that sheds that do not ftractures, in the alabastrum that next day blooms
Carry out emasculation；Described artificial pollination is after female parent every day field dew is dry, fine day 8-9 in morning point, temperature 20～25 DEG C of pollinations；
Described fruit harvesting is 50～60 days mature fruits of gathering after pollination；Described seed treatment is complete red ripe Fruit
After receipts, treat after-ripening 1～take kind again in 2 days；It is with acetone extraction blade astaxanthin, the detection of UPLC method that described content astaxanthin measures
Its content.
The breeding method of tomato variety ' cloud shrimp No. 2 ', collects in wherein said pollen collection step as mentioned
Pollen is temporarily stored with 4 DEG C of refrigerator cold-storages, and long-term storage silica gel is stored in-80 DEG C of refrigerators as desiccant.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein said maternal emasculation step is steady with left hand
Holding alabastrum base portion, right hand tweezers are clamped flower pesticide and are all extractd by its stamen, or together peel off together with petal, notice that emasculation to be done
Only, note isolation after emasculation, reject flower and the selfing fruit being not suitable for pollination simultaneously.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein said artificial pollination step is optimum flat
All temperature is 20～25 DEG C, dips a small amount of pollen point by the rubber tip of common pencil and invests on the stigma of maternal emasculation flower, or
Pollen is directly placed on blank sheet of paper by person, directly contacts the stigma of emasculation, dips a small amount of pollen.With 6 × 8cm tea bag bag bagging, bent
Safety pin is fixed, listing mark, and plate is indicated Parent combination number and pollination date, and bag taking after pollinating 10 days is observed simultaneously
Bear fruit situation, cleaning of pruning in time, remove the alabastrum and the inflorescence of new life do not pollinated, training is pruned, and extracts unnecessary axillalry bud
And the branch of excessive growth, carry out liquid manure coordinated management and the prevention and control of plant diseases, pest control.
The breeding method of tomato variety ' cloud shrimp No. 2 ', life of only gathering in wherein said fruit harvesting step as mentioned
Long normotrophic fruit, do not gather unmarked fallen fruits and malformed fruit.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, horizontal with knife in wherein said seed treatment step
Cut open to by fruit, ferment 1～2 day in seed and glue flesh are clamp-oned anhydrous nonmetallic vessel, be white to fermented product surface, use
Wooden stick stirs, and seed sinks, and pinches seed with hands, and pectin separates with seed, cleans seed, remove sarcocarp, foreign material during without glutinous sliding sense
With flat seed, squeeze the water out in loading double gauze bag, be spread out in the built on stilts canvas in bottom or absorbent paper and dry in the shade, stir several times, make
The moisture of the surface of the seed evaporates rapidly, during until seed moisture content is down to 7%～8%, and paper bag packing, room temperature storage, next year 3
Moon sowing.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, the determination step of wherein said astaxanthin is, waits to plant
Strain is grown up, and gathers the lower 4-5 floor blade in New Tomato Variety ' cloud shrimp No. 2 ' top, freeze-dried acquisition blade dry powder, weighs sample
50mg, adds acetone extraction 4 times, until extract is colourless, collects extract and is settled to 5ml, and after sucking filtration, loading measures pigment, arranges
3 repetitions, use ultra-performance liquid chromatography to measure the content of its astaxanthin.
The breeding method of a kind of tomato variety ' cloud shrimp No. 2 ' of the present invention, it comprises the steps: (1) pollen collection, father
This gathers male parent ' red No. 1 of shrimp ' petal and launches 170-190 ° and flower after dew is dry than maternal planting early enough 20 days, full-bloom stage the morning
The flower that colour cast is red, chooses stamen in indoor tweezers, spread out thin and on the blank sheet of paper of flexible, indicate kind name and gather day
Phase, being placed in clean place and naturally dry in the shade, treat Anther dehiscence, when pollen sheds, collect pollen standby, pollen is stored temporarily in 4
DEG C refrigerator, long-term storage silica gel is stored in-80 DEG C of refrigerators as desiccant；(2) maternal emasculation, pollination selects ' big the previous day
Flat-top ' corolla elongation exposes, and color from green to yellow, do not open or crack by petal, and flower pesticide is yellow green, but the pollen that sheds that do not ftractures,
The alabastrum bloomed next day carries out emasculation, and during emasculation, left hand surely holds alabastrum base portion, and right hand tweezers clamp flower pesticide by complete for its stamen
Portion extracts, or together peels off together with petal, isolates after emasculation, rejects flower and the selfing fruit being not suitable for pollination simultaneously；(3) artificial
Pollination, starts pollination after maternal field dew is dry, fine day the morning 8-9 point carry out, suitable temperature on average of pollinating is 20～25 DEG C,
Dipping a small amount of pollen by the rubber tip of common pencil, point is invested on the stigma of maternal emasculation flower or pollen is placed in blank sheet of paper
On, directly contact the stigma of emasculation, dip a small amount of pollen, with 6 × 8cm tea bag bag bagging, paper clip is fixed, listing mark, board
Parent combination number and pollination date is indicated on son, bag taking after pollinating 10 days, observe situation of bearing fruit simultaneously, training is clear in time
Reason, removes the alabastrum and the inflorescence of new life do not pollinated, and training is pruned, and extracts unnecessary axillalry bud and the branch of excessive growth, carries out liquid manure association
With management and the preventing and treating of pest and disease damage；(4) fruit harvesting, 50～60 days mature fruits of gathering after pollination, just only gathering growth promoter
Normal fruit, do not gather unmarked fallen fruits and malformed fruit；(5) seed treatment, after complete red ripe fruit harvesting, after-ripening 1
～take kind again in 2 days, laterally fruit is cut open with knife, ferments 1～2 day in seed and glue flesh are clamp-oned anhydrous nonmetallic vessel,
Until fermented product superficial white, stirring with wooden stick, seed sinks, and pinches seed with hands, and pectin separates with seed, feels without glutinous cunning
Clean seed, rinse with clear water, remove sarcocarp, foreign material and flat seed, squeeze the water out in loading double gauze bag, be spread out in bottom built on stilts
Canvas or absorbent paper on, stir, make the moisture of the surface of the seed evaporate rapidly, until seed moisture content is down to 7%～8%
Time, paper bag packing, room temperature storage, sow March in next year；(6) content astaxanthin measures, and New Tomato Variety ' cloud shrimp No. 2 ' is after planting
Plant to be planted is grown up, the lower 4-5 layer blade in top of gathering, and freeze-dried acquisition blade dry powder weighs sample 50mg, adds acetone extraction 4
Secondary, until extract is colourless, to collect extract and be settled to 5ml, after sucking filtration, loading measures pigment, arranges 3 repetitions, uses superelevation
Effect liquid phase chromatogram UPLC method measures the content of its astaxanthin.
The breeding method of tomato variety ' cloud shrimp No. 2 ' as mentioned, wherein said is maternal by ' big flat-top ', produces shrimp blue or green
Element engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' is that male parent carries out hybridizing the biological characteristics of ' the cloud shrimp No. 2 ' that obtain and is: ' cloud shrimp No. 2 ' combines
The main merit of Parent, fruit quality is good, and blade high-yield astaxanthin, it is mainly characterized by: plant determinate growth
Type, plant height about 130cm, kermesinus, robust growth；Stem yellow green is to light brown, and branch is very competent；Common blade profile, petiole
Yellow green is to kermesinus, and blade kermesinus, its dry astaxanthin average content reaches 2.27mg/g, and calyx kermesinus is radial
Corolla is orange-yellow, and to Chinese red, flower pesticide is orange to red, stigma and harbor young fruit brownish red, fruit large red, fruit shoulder aubergine,
Circular to oblate, fruit shape index 0.67～0.79, fruit surface smooth, gloss is bright, and sarcocarp is thick, pink colour, and placenta jelly is red, the heart
Room 5～7, hardness is moderate, mean fruit weight 150g, and maximum single fruit weighs 350g.
Compared with prior art, the excellent benefit of the present invention is, the tomato variety cultivated by breeding method of the present invention
' cloud shrimp No. 2 ' reaches 2mg/g as objective trait with dry matter weight of leaf astaxanthin average content, takes into account fruit properties, with plant color
Whether it is that kermesinus is as the mark property differentiating Hybrid and ' big flat-top '.In conjunction with Phenotypic Observation and blade content astaxanthin
Mensuration assessment, ' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high-yield astaxanthin.Its
It is mainly characterized by, plant determinate growth, plant height about 130cm, kermesinus, robust growth；Stem yellow green is the most light brown, branch
Very competent；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches
2.27mg/g.Calyx kermesinus, radial corolla is orange-yellow, and to Chinese red, flower pesticide is orange to red, stigma and harbor young fruit palm fibre
Red.Fruit large red, fruit shoulder aubergine, circular to oblate, fruit shape index 0.67～0.79, fruit surface smooth, gloss is bright, really
Meat is thick, pink colour, and placenta jelly is red, ventricle 5～7, and hardness is moderate, mean fruit weight 150g, maximum single fruit weight up to
350g。
Detailed description of the invention:
Further illustrate the essentiality content of the present invention below by the concrete instance of the present invention, but do not limit with this
Determine the present invention.
Embodiment 1:
The hybridizing method of tomato variety ' cloud shrimp No. 2 ':
With the old kind of Yunnan local ' big flat-top ' for maternal, to produce astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' as paternal hybrid
And obtain.
Maternal ' big flat-top ' Fructus Lycopersici esculenti is the local old kind that Yunnan Province's Chuxiong Prefecture Yuanmou County is planted the earliest, and fruit surface smooth is individual
Head is big, and fruit quality is good, and material to be tested derives from gardens College of Horticulture of Yunnan Prov Agriculture University from the elite plant strain reserved seed for planting.Male parent
' red No. 1 of shrimp ' is to produce astaxanthin engineering Fructus Lycopersici esculenti, cultivated in 2009 and forms, and is that (Huang JC etc., 2013) such as yellow pretty tides pass through
Beta-carotene and zeaxanthin synthesizing astaxanthin in plant cell is screened and be separated to be catalyzed to gene engineering method from chlorella
Assimilation enzyme and '-hydroxylase gene proceed in wild-type tomatoes plant (cv.UC82B), the transgenic Fructus Lycopersici esculenti obtained is (in 2014
On May 14, at the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is: CGMCC No
No. 9223, Classification And Nomenclature: ' red No. 1 of shrimp ' transgenic Fructus Lycopersici esculenti).2010 to 2012 respectively in bioscience institute of Hong Kong University and
Kunming Inst. of Botany, Chinese Academy of Sciences has carried out the plantation of 4 generations by potted plant mode, and character is stable.In March, 2013 starts
Sowing, ' red No. 1 of shrimp ' ratio ' big flat-top ' early sowing 20 days, May, full-bloom stage was pollinated the previous day, the elongation of ' big flat-top ' corolla was exposed,
Color from green to yellow, do not open or crack by petal, and flower pesticide is yellow green, but the pollen that sheds that do not ftractures, the alabastrum bloomed next day goes
Male clean, note isolation.When the morning, dew was done, gather male parent ' red No. 1 of shrimp ' petal expansion 170～190 ° and pattern is the reddest
Flower, choose stamen in indoor tweezers, spread out dry in the air thin and on the blank sheet of paper of flexible, indicate collection the date, be placed in clean ground
Fang Ziran dries in the shade.Treat Anther dehiscence, when pollen sheds, collect pollen standby.Second day, after maternal field dew is dry, fine day was upper
Noon 8-9 point is pollinated.Concrete, dip a small amount of pollen point by the rubber tip of common pencil and invest the stigma of maternal emasculation flower
Above or pollen is placed on blank sheet of paper, the stigma of contact emasculation, directly dips a small amount of pollen.With 6 × 8cm tea bag bag bagging, bent
Safety pin is fixed, listing mark, and plate is indicated Parent combination number and pollination date.Bag taking after pollinating 10 days, observes simultaneously
Bear fruit situation, cleaning of pruning in time, remove the alabastrum and the inflorescence of new life do not pollinated, training is pruned, and extracts unnecessary axillalry bud
And the branch of excessive growth, period notes the preventing and treating of liquid manure coordinated management and pest and disease damage.After pollination 50～60 days mature fruits of can gathering.No
Gather unmarked fallen fruits and malformed fruit, the normal fruit of growth promoter of only gathering.After complete red ripe fruit harvesting, can after
Within ripe 1～2 day, take kind again.When taking kind, laterally fruit is cut open with knife, then seed and glue flesh are clamp-oned anhydrous nonmetallic vessel
Interior fermentation, cleans seed after fermenting 1～2 day, the place being placed in ventilating, being dried is filtered dry moisture dry in the sun immediately, until seed
Water content is down to storage when 7%～8%, sows March in next year.Treat that tomato variety ' cloud shrimp No. 2 ' plant after planting grows up, in good time
Gathering, it pushes up lower 4-5 layer blade, and lyophilization obtains dry powder.Accurately weigh sample 50mg, add acetone extraction 4 times, until extracting
Liquid is colourless, collects extract and is settled to 5ml, and after sucking filtration, loading measures pigment, arranges 3 repetitions, uses Ultra Performance Liquid Chromatography
(UPLC) method measures the content of its astaxanthin.Through 4 plantations of 2014 and 2015, Phenotypic Observation and blade astaxanthin contained
The measurement result of amount shows, ' cloud shrimp No. 2 ' character is excellent neatly, and fruit quality is good, and blade content astaxanthin is high, and disease resistance
By force.
The character of the hybrid strain that hybridizing method of the present invention relates to is as follows:
' big flat-top ': the local old kind that Yunnan Province's Chuxiong Prefecture Yuanmou County is planted the earliest, its plant is determinate growth, strain
High about 120cm, growing way is strong, and stem 8～10 leaf raw 1st inflorescence, belongs to middle-late ripening variety；Strong adaptability, heat-resisting, easily plant
Training, fruit-setting rate is high.Fruit type is neat, oblate, and really face colouring is uniform, and smooth without rib, gloss is bright, mature fruit large red, sarcocarp
Thickness, ventricle 5～7, hardness is moderate.Mean fruit weight 150g, maximum single fruit weight, up to 350g, is planted at Kunming Areas booth in spring
Training, from being seeded into, to gather be about 160 days.
Produce astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ': its plant, flower, leaf, fruit the most morphologically with Lycopersicon esculentum indifference, main
If the difference in color.Specifically, plant determinate growth, plant height about 110cm.Stem half erect type, stem thick 2.6～
4.7cm, 4～6 branches, yellow green is to light brown.Common blade profile, petiole is green to kermesinus, blade kermesinus.Hermaphrodite flower,
Calyx kermesinus, radial corolla is orange-yellow, and to Chinese red, flower pesticide is orange to red, stigma and harbor young fruit brownish red.Fruit
Subsphaeroidal, Chinese red after maturation, mean fruit weight about 100g, the meansigma methods of its fruit content astaxanthin is 1.63mg/g.
New Tomato Variety ' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high yield shrimp
Blue or green element.It is mainly characterized by, plant determinate growth, plant height about 130cm, kermesinus, robust growth；Stem yellow green is to shallow brown
Color, branch is very competent；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content
Reach 2.27mg/g.Calyx kermesinus, radial corolla is orange-yellow, and to Chinese red, flower pesticide is orange to red, stigma and harbor young fruit
Brownish red.Fruit large red, fruit shoulder aubergine, circular to oblate, fruit shape index 0.67～0.79, fruit surface smooth, gloss is bright,
Sarcocarp is thick, pink colour, and placenta jelly is red, ventricle 5～7, and hardness is moderate, mean fruit weight 150g, maximum single fruit weight up to
350g。
Claims (10)
1. the breeding method of tomato variety ' cloud shrimp No. 2 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting, kind
Son processes, content astaxanthin determination step, and with ' big flat-top ' for maternal, producing astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' is that male parent is entered
' cloud shrimp No. 2 ' is cultivated in row hybridization, with acetone extraction blade astaxanthin, and uses UPLC method to detect its content.
2. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 1, it is characterised in that: described pollen collection
It is that male parent gathers male parent ' red No. 1 of shrimp ' petal and launches 170-after dew is dry than maternal planting early enough 20 days, full-bloom stage the morning
190 ° and the reddest flower of pattern, choose stamen, spread out on blank sheet of paper, indicates kind name and gathers the date, being placed in clean place
Naturally dry in the shade, treat Anther dehiscence, when pollen sheds, collect pollen standby；Described maternal emasculation is to select the previous day in pollination
The elongation of ' big flat-top ' corolla exposes, and color from green to yellow, do not open or crack by petal, and flower pesticide is yellow green, but the flower that sheds that do not ftractures
Powder, the alabastrum bloomed next day carries out emasculation；Described artificial pollination is after maternal field dew is done, fine day 8-9 in morning point, temperature
20～25 DEG C of pollinations；Described fruit harvesting is 50～60 days mature fruits of gathering after pollination；Described seed treatment has been
After complete red ripe fruit harvesting, treat after-ripening 1～take kind again in 2n days；It is with acetone extraction blade shrimp that described content astaxanthin measures
Blue or green element, UPLC method detects its content.
3. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 2, it is characterised in that: described pollen collection
The pollen collected in step is temporarily stored with 4 DEG C of refrigerator cold-storages, and long-term storage silica gel is stored in-80 DEG C of ice as desiccant
Case.
4. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 2, it is characterised in that: described maternal emasculation
Step is surely to hold alabastrum base portion with left hand, and right hand tweezers are clamped flower pesticide and all extractd by its stamen, or together shell together with petal
Falling, emasculation is clean, isolates after emasculation, rejects flower and the selfing fruit being not suitable for pollination simultaneously.
5. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 2, it is characterised in that: described artificial pollination
Step temperature is 20～25 DEG C, dips a small amount of pollen point by the rubber tip of common pencil and invests on the stigma of maternal emasculation flower,
Or directly pollen is placed on blank sheet of paper, directly contacts the stigma of emasculation, dip a small amount of pollen.With 6 × 8cm tea bag bag bagging,
Paper clip is fixed, listing mark, and plate is indicated Parent combination number and pollination date, and bag taking after pollinating 10 days is seen simultaneously
Examine the situation of bearing fruit, cleaning of pruning in time, remove the alabastrum and the inflorescence of new life do not pollinated, training is pruned, and extracts unnecessary axil
Bud and the branch of excessive growth, carry out liquid manure coordinated management and the prevention and control of plant diseases, pest control.
6. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 2, it is characterised in that: described fruit harvesting
Step is only gathered the normal fruit of growth promoter, do not gather unmarked fallen fruits and malformed fruit.
7. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 2, it is characterised in that: described seed treatment
Fruit is laterally cut open with knife by step, ferments 1～2 day in seed and glue flesh are clamp-oned anhydrous nonmetallic vessel, to fermentation
Thing surface is white, stirs with wooden stick, and seed sinks, and pinches seed with hands, and pectin separates with seed, cleans and plant during the glutinous sliding sense of nothing
Son, removes sarcocarp, foreign material and flat seed, squeezes the water out in loading double gauze bag, is spread out in the built on stilts canvas in bottom or absorbent paper
Dry in the shade, stir several times, make the moisture of the surface of the seed evaporate rapidly, during until seed moisture content is down to 7%～8%, paper bag bag
Dress, room temperature storage, sow March in next year.
8. the breeding method of tomato variety ' cloud shrimp No. 2 ' as claimed in claim 2, it is characterised in that: described astaxanthin
Determination step is, plant to be planted is grown up, and gathers the lower 4-5 floor blade in New Tomato Variety ' cloud shrimp No. 2 ' top, freeze-dried acquisition blade
Dry powder, weighs sample 50mg, adds acetone extraction 4 times, until extract is colourless, collects extract and is settled to 5ml, loading after sucking filtration
Measure pigment, 3 repetitions are set, use ultra-performance liquid chromatography to measure the content of its astaxanthin.
9. the breeding method of tomato variety ' cloud shrimp No. 2 ', it is characterised in that it comprises the steps: (1) pollen collection, male parent ratio
Maternal planting early enough 20 days, full-bloom stage gathers male parent ' red No. 1 of shrimp ' petal expansion 170-190 ° and pattern is inclined after dew is dry the morning
Red flower, chooses stamen in indoor tweezers, spread out thin and on the blank sheet of paper of flexible, indicate kind name and gather the date, put
Naturally drying in the shade in clean place, treat Anther dehiscence, when pollen sheds, collect pollen standby, pollen is stored temporarily in 4 DEG C of ice
Case, long-term storage silica gel is stored in-80 DEG C of refrigerators as desiccant；(2) maternal emasculation, pollination selects ' big flat-top ' the previous day
Corolla elongation exposes, and color from green to yellow, do not open or crack by petal, and flower pesticide is yellow green, but the pollen that sheds that do not ftractures, next day
The alabastrum bloomed carries out emasculation, and during emasculation, left hand surely holds alabastrum base portion, and right hand tweezers are clamped flower pesticide and all plucked by its stamen
Remove, or together peel off together with petal, isolate after emasculation, reject flower and the selfing fruit being not suitable for pollination simultaneously；(3) manually award
Powder, starts pollination after maternal field dew is dry, fine day the morning 8-9 point carry out, suitable temperature on average of pollinating is 20～25 DEG C, use
The rubber tip of common pencil dips a small amount of pollen, and point is invested on the stigma of maternal emasculation flower or is placed on blank sheet of paper by pollen,
Directly contacting the stigma of emasculation, dip a small amount of pollen, with 6 × 8cm tea bag bag bagging, paper clip is fixed, and listing mark, on plate
Indicate Parent combination number and pollination date, bag taking after pollinating 10 days, observe situation of bearing fruit, cleaning of pruning in time simultaneously, go
Except the alabastrum do not pollinated and the inflorescence of new life, training is pruned, and extracts unnecessary axillalry bud and the branch of excessive growth, carries out liquid manure coordinated management
Preventing and treating with pest and disease damage；(4) fruit harvesting, 50～60 days mature fruits of gathering after pollination, the normal fruit of growth promoter of only gathering
Real, do not gather unmarked fallen fruits and malformed fruit；(5) seed treatment, after complete red ripe fruit harvesting, after-ripening 1～2 days are again
Take kind, laterally fruit is cut open with knife, ferment 1～2 day in seed and glue flesh are clamp-oned anhydrous nonmetallic vessel, until fermentation
Thing superficial white, stirs with wooden stick, and seed sinks, and pinches seed with hands, and pectin separates with seed, without glutinous sliding sense kind the most capable of washing
Son, rinses with clear water, removes sarcocarp, foreign material and flat seed, squeezes the water out in loading double gauze bag, is spread out in the canvas that bottom is built on stilts
Or in absorbent paper, stir, make the moisture of the surface of the seed evaporate rapidly, during until seed moisture content is down to 7%～8%, paper bag
Packaging, room temperature storage, sow March in next year；(6) content astaxanthin measures, and New Tomato Variety ' cloud shrimp No. 2 ' after planting plant to be planted is long
Greatly, the lower 4-5 layer blade in top of gathering, freeze-dried acquisition blade dry powder, weigh sample 50mg, add acetone extraction 4 times, until taking out
Extract is colourless, collects extract and is settled to 5ml, and after sucking filtration, loading measures pigment, arranges 3 repetitions, uses ultra high efficiency liquid phase color
Spectrum UPLC method measures the content of its astaxanthin.
10. the breeding method of the tomato variety ' cloud shrimp No. 2 ' as described in claim 1 or 9, it is characterised in that described by ' big
Flat-top ' it is maternal, producing astaxanthin engineering Fructus Lycopersici esculenti ' red No. 1 of shrimp ' is the biological characteristics that male parent carries out hybridizing ' the cloud shrimp No. 2 ' that obtain
Property is: ' cloud shrimp No. 2 ' is combined with the main merit of Parent, and fruit quality is good, and blade high-yield astaxanthin, and it is the most special
Levy as plant determinate growth, plant height 170-190cm, kermesinus, robust growth；Stem yellow green is the most light brown, branch ability pole
By force；Common blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.27mg/g, flower
Calyx kermesinus, radial corolla is orange-yellow, and to Chinese red, flower pesticide is orange to red, stigma and harbor young fruit brownish red, and fruit is big
Redness, fruit shoulder aubergine, circular to oblate, fruit shape index 0.67～0.79, fruit surface smooth, gloss is bright, and sarcocarp is thick, pink colour,
Placenta jelly is red, ventricle 5～7, and hardness is moderate, mean fruit weight 150g, and maximum single fruit weighs 350g.
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| CN109964580A (en) * | 2017-12-28 | 2019-07-05 | 云南生物谷药业股份有限公司 | A kind of seed growing method of high cycleanine and cepharanthine content Stephania epigaea |
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| US20030126649A1 (en) * | 2002-01-03 | 2003-07-03 | Kuehn Michael Edwin | Inbred tomato line 294 |
| CN104145678A (en) * | 2014-08-15 | 2014-11-19 | 中国科学院昆明植物研究所 | Cultivation method for promoting growth of transgenic tomatoes and accumulation of astaxanthin |
| CN104206139A (en) * | 2014-08-15 | 2014-12-17 | 中国科学院昆明植物研究所 | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato |
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| CN104206139A (en) * | 2014-08-15 | 2014-12-17 | 中国科学院昆明植物研究所 | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato |
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| CN106577254A (en) * | 2016-11-30 | 2017-04-26 | 福建省农业科学院茶叶研究所 | Tool set and pollination method for increasing artificial pollination efficiency of tea trees |
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| CN109964580A (en) * | 2017-12-28 | 2019-07-05 | 云南生物谷药业股份有限公司 | A kind of seed growing method of high cycleanine and cepharanthine content Stephania epigaea |
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