KR101881992B1 - New chinese cabbage variety - Google Patents

Abstract

To a new variety of Chinese cabbage plants and parts thereof.

Description

New variety Chinese cabbage plant {New chinese cabbage variety}

To a new variety of Chinese cabbage plants and parts thereof.

Chinese cabbage campestris spp. Pekinensis) is a plant belonging to the crucifers and Chinese cabbages. Chinese cabbage is one of the most used vegetables in Korea as a major ingredient of kimchi. It has a high moisture content and is rich in fiber and vitamins A and C.

The leaves of Chinese cabbage are spiral-shaped and shortened in stem shape to have a rosette shape. Leaves from roots are light green, and the flowers of Chinese cabbage are dark yellow crucifixes, including one pistil and six stamens. Chinese cabbage has a characteristic that it grows when it becomes a high temperature long day after a flower bud occurs at a low temperature.

Varieties of Chinese cabbages are classified according to the type of plant or cultivation period. Fuller species that are full of foliage are divided into a fuller type in which the leaves are gathered in the central part but not overlapping ends, and a foreskin type in which the leaves grow beyond the central part and cover the ends like cabbage.

The development of cabbage varieties with higher product value and higher utility is required.

One aspect provides a new variety of Chinese cabbage plants.

Another aspect provides a leaf, stem, seed, or cell obtained from the plant.

One aspect is that a new variety of Chinese cabbage ( Brassica campestris ssp . pekinensis) plants.

In the plant, the height of the plant may be a height measured at the same time point for a control cabbage grown under the same conditions as an average height. The height of the plant may be measured at 85 days to 110 days from the date of planting the Chinese cabbage, for example, at the autumn. The height of the plant may be measured at the maturity stage of autumn cultivation of Chinese cabbage. The term 'maturity' may mean the completion phase of the Chinese cabbage, and the Chinese cabbage that has reached maturity may be in a state where it can be harvested. The plant may be one in which the leaf is differentiated and grows, and then the leaves are filled and filled to form a sphere. The step of completing the conclusion may be a step in which the conclusion is not further advanced. The height of the plant may be 10% or more, for example, 20% or more, or 30% or more of the control cultivar. The plant height may be 10% to 30%, 15% to 30%, or 10% to 20% larger than the control varieties, for example. The height of the plant may be 40 cm to 55 cm at the maturity stage. The plant may be cultivated at a temperature ranging from -3 ° C to 30 ° C. The plant may be sown in the Korean weather from July to August, for example from August 1 to August 20. The control varieties may be bulbamplus.

Unless otherwise stated in the present specification, the characteristics of the cabbage cultivars are determined by the requirements of Article 30 of the New Plant Protection Act and Article 33 of the Enforcement Decree of the same Act, "Chinese cabbage (Brassica campestris L. spp. Pekinensis (Lour.) Rupr.)", Which specifies the rules for conducting the characterization tests required for the cultivation review under Article 47, (Ministry of Agriculture and Forestry, National Seed Management Office, 2005: http./www.seed.go.kr) ". Unless otherwise noted, the quantitative traits of the characteristics of the cabbage cultivars represent an average value.

In the plant, the height of the sphere may be a height measured at the same time point for a control cabbage grown under the same conditions as an average height. The height of the sphere may be measured between 85 days and 110 days from the day when the Chinese cabbage is sown. The height of the sphere may be measured at the mature stage of the Chinese cabbage. The height of the sphere may be at least 10%, at least 15%, or at least 20% greater than the control varieties. The height of the sphere may be 10% to 15%, 15% to 20%, or 20% to 30% larger than the control varieties, for example. The height of the sphere may be 30 cm to 40 cm at the mature stage. The control varieties may be bulbamplus.

The plant may have the following morphological characteristics.

(1) The outer leaf color is dark green.

(2) The leaf length is long.

(3) Plant height is large.

(4) Plant growth habits are generally semi-elongatable.

(5) The sphere has a long oval shape. In other words, it is a form similar to a needle.

(6) The stomach of the sphere shows a little proptogenicity.

(7) The color of the sphere is 4.0 (1 = white ~ 5.0 = orange) in the ripening stage of autumn cultivation.

The plant may be one having characteristics distinct from the control varieties described below. The control cultivar is Bulam Plus.

(1) The outer leaf length is longer than the control varieties.

(2) The outer leaf width is narrower than the control varieties.

(3) Appearance of Outer Leaf The filed varieties are long oval and contrast varieties are inverted oval.

(4) The tip of the outer leaf is filed. The varieties are flat and the control varieties are round.

(5) The unevenness of the outer leaves is more severe than the control varieties.

(6) The intensity of the color of the outer leaf is thicker than the control varieties.

(7) The hairs of the outer leaves are less than those of the control varieties.

(8) The wavy shape of the edge of the outer leaf is stronger than the control varieties.

(9) The jagged edge of the outer leaf is stronger than the control varieties.

The plant may be derived from a seed deposited with Accession No. KCTC 12954BP. The new variety of Chinese cabbage plants of the present invention was designated as "Brassica campestris spp. Pekinensis Mi In 1ho" and the seeds were transferred to the KCTC Microorganism Resource Center, an international depository institution under the Budapest Treaty on December 2, 2015, as KCTC 12954BP ≪ / RTI >

Another aspect provides a plant part of the plant or a progeny plant derived therefrom. The sub-body may be selected from the group consisting of protoplasts, ovules, cells, pollen grains, embryos, cotyledons, hypocotyledons, roots, meristems, ), A stem, or a leaf. The derived offspring plants include seeds, or tissue cultures. The leaves and / or stems obtained from the Chinese cabbage plants can be used variously as food materials such as kimchi, herbs and salads. The seeds and / or cells obtained from the Chinese cabbage plants may be used to grow other cabbage plants, lines, or cultivars.

The above-mentioned Chinese cabbage plant is more suitable for producing pickled foods such as kimchi because the height of the plant is high and the height of the sphere is large and the shape of the vertically cut face is elliptic, compared with the existing Chinese cabbage variety 'Bulam Plus'. That is, the yield is more than 10% by weight on the basis of the control varieties. The Chinese cabbage plant has a small core part, which is smaller than 5% by weight, and thus has a high yield of kimchi.

The Chinese cabbage plants were cultivated in a small amount and the individuals were selected as non-M-68, and the leaves were large and dark green, no symptoms were observed, no embryogenesis was observed, In this study, we investigated the effect of the M-43 and M-43 on the growth and development of microorganisms. It was selected by confirming that the sphere is large and beautiful and the quantity is large.

Another aspect provides a method of producing the Chinese cabbage plant, comprising seeding and growing the seed of the Chinese cabbage plant in a soil or nutrient medium.

According to one aspect, the plant of the Chinese cabbage variety is high in plant height and tall in the sphere, and the shape of the vertically cut surface is long oval shape, so that the product value is higher than that of the existing cabbage variety and can be used as a food material for various purposes.

The leaves, stems, seeds, or cells obtained from the Chinese cabbage plants according to one aspect can be used to produce the Chinese cabbage plants or produce foods using them.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 shows a breeding history chart of the cabbage cultivars of the present invention. Fig.
2 shows a breeding diagram of the cabbage cultivar of the present invention.
3 is a view showing the Chinese cabbage cultivars and the control varieties of the present invention.
Fig. 4 is a view showing vertically cut surfaces of the Chinese cabbage cultivars and control cultivars cultivated in the local cultivation area.
Fig. 5 is a view showing a view of the cultivated cabbage cultivars and control varieties of the invention cultivated in the cultivation areas of the Hainan area of Jeonnam.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments and are not intended to limit the scope of the present invention.

Example  1: Cultivating new varieties

Unless otherwise noted, microbial cultivation, crossbreeding and selection procedures for cultivating the following new variety of cabbage were carried out from January 2003 to November 2015 in Daedeok-myeon, Anseong-si, Gyeonggi-do. The region has an average annual temperature of 24.7 ° C from 2013 to 2015 and an annual average minimum temperature of 12.3 ° C. The average annual minimum temperature is the average of the daily minimum temperatures. The average annual precipitation was cultivated in the area having the characteristics of 1033 mm from 2013 to 2015.

(1) Material collection and system upbringing

Material collection and system cultivation for oilseed of the breed of the present invention was carried out through micropore culture.

Specifically, in 2003, boram 3, which is one of the most popular varieties available in the market, was purchased and disclosed to cultivate micro - organisms for the purpose of early cultivation. A total of 37 doubled haploids (DH) were characterized in the autumn of 2005-2006. The leaves were large and dark green, no symptoms were observed, no embryo development was observed, and 68 leaves were selected as myopteran and extreme life span. This line was named M-68. Afterwards, the system was tested in combination with several other systems.

In addition, the varieties of long oval type, called 형 type, introduced in China in 2002, have a leaf color of green color, a long oval shape, a mildew of 55 days, . Therefore, in order to cultivate the varieties of beech cabbage, it was announced in 2004 to cultivate 5 lines. As a result of the cultivation test, the system with no disease on the leaves and the employee's sickness and the yellowing was selected and named as M-43 in 2007.

Culture and characterization of the microspore cultivation was conducted according to the following procedure.

(1.1) Cultivation of microspheres

Bulam 3 and Post 55 were planted in the soil. The plants were cultivated in a greenhouse at a temperature of 5 ℃ for about 30 days. For extraction of the microspores, the induced buds were sampled and only the buds longer than the petals (also called "buds") were selected. The selected buds were surface-sterilized in 1% sodium hypochlorite solution for 15 minutes and then rinsed three times for 3 minutes with sterilized water. The sterilized buds were placed in a bowl and 2 to 3 ml of B5 medium supplemented with 13% sucrose (Gamborg et al., Experimental Cell Research, 1968, vol. 50, no. 1, , And the microparticle solution was filtered through a 45 mu m sieve. The filtered solution was further added with B5 medium supplemented with 13% sucrose, the volume was adjusted to 10 ml, and the tube was centrifuged at 1,000 rpm for 3 minutes. The supernatant was discarded and the B5 medium was washed twice . The washed microspheres were suspended in a 1: 2 NLN (Lichter R. et al., Z. Pflanzenphysiol., 1982, 105, 427-434) liquid medium The concentration was adjusted to 50,000 cells / mL. The well-mixed microsomal solution was dispensed into a Petri dish (60 × 15 mm) with a pipette in 3 mL increments, followed by triple sealing with parafilm.

(1.2) Regeneration into plants

The sealed petri dish was incubated for 1 day at an initial incubation temperature of 32.5 ° C, and then induced to grow for 9 days at 25 ° C at room temperature. After incubation, the cells were transferred to a shaking incubator and further cultured at 70-80 rpm for 10 days. The formed pellets were transferred to a basic solid medium (MS: Murashige and Skoog, 1962, Physiol. Plant 15 (3): 473-497) supplemented with 0.25% gelrite and incubated at 25 ± 2 ° C for 16 hours / For 4 weeks. The induced seedlings were planted in 8 cm pots with horticultural soil and purified for 3 weeks using a net image (L 119 cm × H 35 cm × W 74 cm).

(1.3) Cultivation test

The purified plants were subjected to low temperature treatment at 5 ° C for 30 days in order to induce flowering, and then planted in a large flowerpot (H30.5 cm × ID 26.2 cm × Bottom ID 20 cm) containing horticultural soil and cultivated in a vinyl house, The characteristics were tested. In particular, the lines derived from the cultivation of the bullae of No. 3 and No. 55 were named M-68 and M-43.

(2) Combination creation and verification

Since 2007, various combinations using these two systems have been developed and tested. Then, in 2013, the combination of the two systems, ie, M-68 x M-43 Combinations were created and validated. As a result, it was selected as excellent combination with long oval shape which is not circulated in Korea until now and which is large and beautiful in quantity and high in quantity.

It was planted and cultivated on August 10, the proper time of autumn test, and it was officially confirmed in field around September 5. As a result, the yield was more than 10% by weight on the basis of the control cultivar on November 10, And the like.

(3) Direct adaptive test

Fire M-68 x M-43 Since the combination was finally selected, the two regions of Goesan, Goesan, Chonbuk, and Haenam, Chonbuk province were officially planted on August 20 and September 2, and harvested on October 22 and November 7, respectively. In addition, in 2015, in Goesan, Chungcheongbuk-do and Chonnam, Haenam, in the same region as July 2013 and August 3, respectively, August 28 and September 8, and November 10 and November 11 And harvested. The two-year fall test was stable.

(4) Characteristics of cultivated varieties of the present invention

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 shows a breeding history chart of the cabbage cultivars of the present invention. Fig. As shown in Fig. 1, the Chinese cabbage cultivar of the present invention was cultivated from 2003 to 2015.

2 shows a breeding diagram of the cabbage cultivar of the present invention. As shown in FIG. 2, the Chinese cabbage cultivar of the present invention was cultivated through the non-M-68 strain breeding, the M-43 strain breeding, the combination test and the farm demonstration test. Specifically, in 2002, the company acquired the number 3 varieties of Bulam 3 and announced to cultivate the microspore for the purpose of cultivating the excellent purple in 2003. In 2004, it selected 24 strains, Respectively. In addition, the varieties of Supplement No. 55 in 2003 were collected and announced in 2004 for the cultivation of the microspheres. In 2005, 5 strains were selected and the system was named as M-43, . In 2013, M-68 × M-43 combination test will be selected for the first time, and the best combination of long oval shape will be selected. The 2014 ~ 2015 will be applied to Chinese cabbage farmers in Goesan, Chonbuk and Chonnam province. It was named as the first beautiful cabbage variety.

Table 1 shows the results of comparing the number 1 of the Chinese cabbage cultivars of the present invention and the control cultivar Boram plus.

 The results shown in Table 1 were obtained by harvesting on November 10, 94 days after sowing, which was determined to be the optimum time for harvesting after sowing and cultivating on August 7 in Anseong, Gyeonggi Province. The cultivation was carried out in the open field. In Table 1, characteristics 1. Outer leaf: posture, characteristic 5. Outer leaf: shape, characteristic 19. Outer leaf: length of the middle rib, and characteristic 24. Sphere: 8, and Fig. 9, respectively.

As shown in Table 1, the Chinese cabbage cultivar of the present invention has the following morphological characteristics.

(1) The outer leaf color is dark green.

(2) The leaf length is long.

(3) Plant height is large.

(4) Plant growth habits are generally semi-elongatable.

(5) The sphere has a long oval shape. In other words, it is a form similar to a needle.

(6) The stomach of the sphere shows a little proptogenicity.

(7) The color of the sphere is about 4.0 (1 = white ~ 5.0 = orange) in the ripening period of the autumn cultivation.

In addition, the Chinese cabbage cultivar of the present invention may have characteristics different from the control cultivar Boram plus.

(1) The outer leaf length is longer than the control varieties.

(2) The outer leaf width is narrower than the control varieties.

(3) Appearance of Outer Leaf The filed varieties are long oval and contrast varieties are inverted oval.

(4) The tip of the outer leaf is filed. The varieties are flat and the control varieties are round.

(5) The unevenness of the outer leaves is more severe than the control varieties.

(6) The intensity of the color of the outer leaf is thicker than the control varieties.

(7) The hairs of the outer leaves are less than those of the control varieties.

(8) The wavy shape of the edge of the outer leaf is stronger than the control varieties.

(9) The jagged edge of the outer leaf is stronger than the control varieties.

3 is a view showing the Chinese cabbage cultivars and the control varieties of the present invention. In Fig. 3, the left side is the control varietal bulbamplus, and the right side is the cabbage cultivar No. 1 of the present invention. The Chinese cabbage in Fig. 3 was planted in Anseong-si, Gyeonggi-do, which was sown on August 7, 2015 and harvested on November 10th. As shown in Fig. 3, the cabbage cultivars of the present invention are long oval in shape such that the plant is taller and the height of the sphere is larger than that of the control varieties, and the shape of the vertically cut surface is like a needle.

Fig. 4 is a view showing vertically cut surfaces of the Chinese cabbage cultivars and control cultivars cultivated in the local cultivation area. 4, the left side is the control varieties, and the right side is the cabbage varieties No. 1 of the present invention. The Chinese cabbage of FIG. 4 was planted in the Goesan area, which was sown on July 31, 2015 and harvested on November 10, and was grown in the autumn. As shown in Fig. 4, the cabbage cultivars of the present invention are larger in plant height and larger in height than the control varieties, and have a long oval shape in the shape of a vertically cut face. Accordingly, the cabbage cultivar of the present invention showed an increase of 10% or more in weight on the weight basis as compared with the control varieties.

 FIG. 5 is a view showing a view of a plant of a Chinese cabbage cultivar cultivated in a plantation area in the Hainan region of Chonnam Province. The Chinese cabbage of Fig. 5 was planted on July 31, 2015, and harvested on November 11, and grown in autumn. As shown in Fig. 5, the cabbage cultivars of the present invention are uniform.

The characteristics of the Chinese cabbage cultivars shown in Table 1 are as follows: Table 1 of Annex 1 of Seed Management Guideline Article 30 of the New Plant Protection Act and Article 33 of the Enforcement Decree of the same Act; (Chinese cabbage) (Brassica campestris L. spp. Pekinensis (Lour.) Rupr) (Ministry of Agriculture, Forestry and Fisheries, National Institute of Advanced Industrial Science and Technology) Seed Management Station, 2005: http./www.seed.go.kr) ". Unless otherwise noted, the quantitative traits of the characteristics of the cabbage cultivars represent an average value.

Korean Collection for Type Cultures KCTC12954BP 20151202

Claims (5)

A new variety of Chinese cabbage ( Brassica campestris ssp . Pekinensis) plant, which has a larger plant height and a larger tiller than its control varieties, Bulam Plus,
Wherein the plant is deposited with the seed deposit number KCTC 12954BP. The plant according to claim 1, which has the following characteristics in comparison with the control cultivar Boram-plus:
(1) The outer leaf length is longer than the control varieties.
(2) The outer leaf width is narrower than the control varieties.
(3) Appearance of Outer Leaf The filed varieties are long oval and contrast varieties are inverted oval.
(4) The tip of the outer leaf is filed. The varieties are flat and the control varieties are round.
(5) The unevenness of the outer leaves is more severe than the control varieties.
(6) The intensity of the color of the outer leaf is thicker than the control varieties.
(7) The hairs of the outer leaves are less than those of the control varieties.
(8) The wavy shape of the edge of the outer leaf is stronger than the control varieties.
(9) The jagged edge of the outer leaf is stronger than the control varieties. delete A plant part of the plant of claim 1. The method according to claim 4, wherein the protoplast, the ovule, the cell, the pollen grain, the embryo, the cotyledon, the hypocotyledon, the root, the anther, Seed part body.

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