CN106106121B - A kind of breeding method of leaf production astaxanthin tomato variety - Google Patents
A kind of breeding method of leaf production astaxanthin tomato variety Download PDFInfo
- Publication number
- CN106106121B CN106106121B CN201610003043.4A CN201610003043A CN106106121B CN 106106121 B CN106106121 B CN 106106121B CN 201610003043 A CN201610003043 A CN 201610003043A CN 106106121 B CN106106121 B CN 106106121B
- Authority
- CN
- China
- Prior art keywords
- fruit
- cloud
- shrimp
- red
- astaxanthin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The breeding method of New Tomato Variety ' cloud shrimp No. 1 ', so that ' mango 1 ' and CLN2037E filial generation ' cloud is anti-No. 1 ' is female parents, production astaxanthin engineering tomato ' red No. 1 of shrimp ' is that male parent is hybridized, and its content is detected with acetone extraction blade astaxanthin, and using UPLC methods.' cloud shrimp No. 1 ' is combined with the main merit of Parent, and disease resistance is strong and high-yield astaxanthin.It is mainly characterized by, plant one dimensional infinite wall, more than plant height 200cm, and growth potential is extremely strong；Anti- late blight, early blight and virosis；Stem green is to kermesinus, and branch ability is strong, and leaf yield is high；Common blade profile, petiole yellow green to kermesinus, vein yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reach 2.02mg/g.Calyx yellow green is to kermesinus, and radial corolla yellow to Chinese red, flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit is orange red, and fruit shoulder aubergine, oblong, fruit shape index 1.07~1.25, fruit surface smooth, gloss is bright, and pulp is thick, and ventricle 4, hardness is big, and mean fruit weight 120g, maximum single fruit weight is up to more than 200g.
Description
Technical field：
The invention belongs to biological technical field, and in particular to the breeding method of tomato plants Hybrid, more specifically, relating to
And with ' mango -1 ' be it is maternal, ' CLN2037E ' be ' cloud resists No. 1 ' that paternal hybrid comes be female parent, to produce astaxanthin engineering kind
Eggplant ' red No. 1 of shrimp ' is that paternal hybrid obtains the breeding method that a kind of leaf produces the tomato triple hybrid of astaxanthin.
Background technology：
It ' mango -1 ', is to be selected by tomato project team of gardens College of Horticulture of Yunnan Prov Agriculture University through 3 years that ' cloud is anti-No. 1 ' female parent, which is,
The self-mating system educated, there is the characters such as high anti-late blight, early blight and virosis, high yield, fruit orange colour, a single fruit weight 200g left sides
It is right.
' cloud is anti-No. 1 ' male parent is ' CLN2037E ', is to be introduced by Asia Vegetable Research centre of development (Taiwan), through Yunnan agriculture
The self-mating system that the generation of tomato project team of study column woods College of Horticulture of sparetime university 3 obtains after purification, there is high anti-late blight, early blight and virus
The characters such as disease, high yield, fruit are large red, and single fruit weight is 60g or so.
' cloud is anti-No. 1 ' is so that ' mango -1 ' is maternal, ' CLN2037E ' obtains for paternal hybrid, is by Yunnan in 2007 years
Tomato project team of gardens College of Horticulture of agriculture university lasts seed selection in 3 years, and its plant is one dimensional infinite wall, more than plant height 200cm,
Grow strong, category middle-late ripening variety；Fruit large red, uniform coloring, oblong, fruit surface smooth, gloss is bright, and pulp is thick, the heart
Room 4, hardness is big, and storage tolerance is good, shelf life length, mean fruit weight 120g, and maximum single fruit weight can be up to more than 200g, in elder brother
Bright regional spring greenhouse cultivation, it is 180 days or so from harvesting is seeded into.
Produce astaxanthin engineering tomato ' red No. 1 of shrimp ' be its plant, flower, leaf, fruit morphologically with Lycopersicon esculentum indifference, it is main
If the difference in color.Specifically, plant one dimensional infinite wall, plant height 110cm or so.The erect type of stem half, stem thick 2.6~
4.7cm, 4~6 branches, yellow green is to light brown.Common blade profile, petiole green to kermesinus, blade kermesinus.Calyx is dark red
Color, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit is subsphaeroidal, into
Chinese red, mean fruit weight 100g or so after ripe, the average value of its fruit content astaxanthin is 1.63mg/g.
With ' mango -1 ' be it is maternal, ' CLN2037E ' be ' cloud resists No. 1 ' that paternal hybrid comes be female parent, to produce astaxanthin
Engineering tomato ' red No. 1 of shrimp ' is cross-breeding for male parent, so as to obtain a kind of cultivation side of leaf production astaxanthin New Tomato Variety
There is not been reported with technology for method.
The content of the invention：
It is an object of the invention to provide one kind so that ' mango -1 ' is maternal, ' CLN2037E ' kind for coming for paternal hybrid
Eggplant ' cloud anti-No. 1 ' is female parent, and production astaxanthin engineering tomato is cross-breeding for male parent, obtains that a kind of disease resistance is strong, leaf high yield
The breeding method of the New Tomato Variety of astaxanthin.
The above-mentioned purpose of the present invention is realized by following technical scheme：
The breeding method of tomato variety ' cloud shrimp No. 1 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting,
Seed treatment, content astaxanthin determination step, using by ' mango -1 ' is ' cloud anti-1 that maternal, CLN2037E obtains as paternal hybrid
Number ' for female parent, production astaxanthin engineering tomato ' red No. 1 of shrimp ' it is that male parent is hybridized, with acetone extraction blade astaxanthin, and use
UPLC methods detect its content.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein described pollen collection step is, male parent is than female
This early sowing 20 days, the full-bloom stage morning, collection male parent ' red No. 1 of shrimp ' petal deploys 170-190 ° and pattern is partially red after dew is dry
Flower, chooses stamen, is spread out on paper, indicates kind name and collection date, dries in the shade naturally, treat Anther dehiscence, when pollen sheds, receives
It is standby to collect pollen；Described maternal emasculation is, pollination the previous day selection ' cloud is anti-No. 1 ' corolla elongation is exposed, color from green to yellow,
Petal is not opened or crack, flower pesticide carries out emasculation in shed pollen, the bud bloomed in next day of yellow green but do not ftracture；Described
Artificial pollination be maternal field dew it is dry after, pollinated in fine day morning 8-9 points, 20~25 DEG C of temperature；Described fruit harvesting is
50~60 days harvesting ripening fruits after pollination；Described seed treatment is after-ripening 1~2 day after complete red ripe fruit harvesting
Kind is taken again；Described content astaxanthin measure is to use acetone extraction blade astaxanthin, and UPLC methods detect its content.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein collected in described pollen collection step
Pollen is temporarily stored in 4 DEG C of refrigerators, and long-term storage silica gel is made drier and preserved in -80 DEG C of refrigerators.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein described maternal emasculation step is steady with left hand
Bud base portion is held, the right hand clamps flower pesticide with tweezers and all extracts its stamen, or is together peeled off together with petal, and emasculation is clean, goes
Isolate after hero, while reject the flower for being not suitable for pollination and selfing fruit.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein described artificial pollination step is at 20~25 DEG C,
A small amount of pollen point is dipped with the rubber tip of common pencil to invest on the column cap of maternal emasculation flower, or is directly placed in pollen white
On paper, the column cap of emasculation is directly contacted, dips a small amount of pollen, with 6 × 8cm tea bag bag baggings, paper clip is fixed, listing mark,
Parent combination number and pollination date are indicated on plate, bag taking after pollinating 10 days, observes situation of bearing fruit, in time training cleaning,
Remove the bud do not pollinated and the inflorescence of new life, training are pruned, extract unnecessary axillary bud and the branch of excessive growth, carry out liquid manure collaboration
Management and the preventing and treating of pest and disease damage.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein only harvesting growth hair in described fruit harvesting
Normal fruit is educated, does not harvest unmarked fallen fruits and malformed fruit.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein in described seed treatment step that fruit is horizontal
To splitting, seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, be white to fermentate surface, stirred with wooden stick
Dynamic, seed sinks, and seed is pinched with hand, and pectin separates with seed, without seed is cleaned when sticking sliding sense, removes pulp, debris and flat seed,
Load in double gauze bag and squeeze the water out, be spread out on the overhead canvas in bottom or blotting paper and dry in the shade, stir several times, make the surface of the seed
Moisture evaporate rapidly, until seed moisture content is when being down to 7%~8%, paper bag packing, room temperature storage, sowing in March in next year.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein the determination step of described astaxanthin is to wait to plant
Strain is grown up, and the lower 4-5 floor blades in collection New Tomato Variety ' cloud shrimp No. 1 ' top, freeze-dried acquisition blade dry powder, weighs sample
50mg, adds acetone extraction 4 times, until extract is colourless, collects extract and is settled to 5ml, loading determines pigment after suction filtration, sets
3 repetitions, the content of its astaxanthin is determined using ultra performance liquid chromatography UPLC methods.
The breeding method of tomato variety ' cloud shrimp No. 1 ', it comprises the following steps：(1) pollen collection, male parent is than maternal early sowing
20 days, the full-bloom stage morning, collection male parent ' red No. 1 of shrimp ' petal deployed 170-190 ° and the partially red flower of pattern after dew is dry, in
Indoor tweezers choose stamen, spread out on thin and flexible blank sheet of paper, indicate kind name and collection date, are placed in clean ground
Fang Ziran dries in the shade, and treats Anther dehiscence, and when pollen sheds, collection pollen is standby, and pollen is stored temporarily in 4 DEG C of refrigerators, long-term storage
It is stored in silica gel as drier in -80 DEG C of refrigerators；(2) maternal emasculation, pollination the previous day selection ' cloud is anti-No. 1 ' corolla elongation are outer
Dew, color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, the bud bloomed in next day
Emasculation is carried out, during emasculation, left hand surely holds bud base portion, and the right hand clamps flower pesticide with tweezers and all extracts its stamen, or together with flower
Valve together peels off, and isolates after emasculation, while rejects the flower for being not suitable for pollination and selfing fruit；(3) artificial pollination, daily maternal field
Start to pollinate after dew is dry, fine day is carried out in morning 8-9 points, and suitable temperature on average of pollinating is 20~25 DEG C, with common pencil
Rubber tip dip a small amount of pollen, point is invested on the column cap of maternal emasculation flower or pollen is placed on blank sheet of paper, directly contact
The column cap of emasculation, a small amount of pollen is dipped, with 6 × 8cm tea bag bag baggings, paper clip is fixed, listing mark, and father and mother are indicated on plate
This combination number and pollination date, bag taking after pollinating 10 days, while situation of bearing fruit is observed, training cleaning in time, remove and do not pollinate
Bud and new life inflorescence, training prunes, and extracts unnecessary axillary bud and the branch of excessive growth, carries out liquid manure coordinated management and disease pest
Harmful preventing and treating；(4) fruit harvesting, 50~60 days harvesting ripening fruits, do not harvest unmarked fallen fruits and malformed fruit after pollination,
Only harvest the normal fruit that grows；(5) after seed treatment, completely red ripe fruit harvesting, after-ripening takes kind for 1~2 day again, uses
Knife laterally splits fruit, and seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, until fermentate surface
White, stirred with wooden stick, seed sinks, and seed is pinched with hand, and pectin separates with seed, without seed is cleaned when sticking sliding sense, uses clear water
Rinsing, pulp, debris and flat seed are removed, load in double gauze bag and squeeze the water out, be spread out in the overhead canvas in bottom or blotting paper
On, stir, the moisture of the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%~8%, paper bag packing, normal temperature
Storage, sowing in March in next year；(6) content astaxanthin determines, and New Tomato Variety ' cloud shrimp No. 1 ' after planting grow up by plant to be planted, harvesting top
Lower 4-5 layer blades, freeze-dried acquisition blade dry powder, weigh sample 50mg, add acetone extraction 4 times, until extract is colourless,
Collect extract and be settled to 5ml, loading determines pigment after suction filtration, sets 3 repetitions, is surveyed using ultra performance liquid chromatography UPLC methods
The content of its fixed astaxanthin.
The breeding method of tomato variety ' cloud shrimp No. 1 ' as mentioned, wherein it is described with by ' mango -1 ' for it is maternal,
CLN2037E is that ' cloud is anti-No. 1 ' that paternal hybrid obtains is maternal, and production astaxanthin engineering tomato ' red No. 1 of shrimp ' is that male parent progress is miscellaneous
Hand over and cultivate the obtained biological characteristics of ' cloud shrimp No. 1 ' and be：' cloud shrimp No. 1 ' is combined with the main merit of Parent, disease-resistant
Property strong and high-yield astaxanthin.It is mainly characterized by, plant one dimensional infinite wall, more than plant height 200cm, robust growth, growth potential pole
By force；Anti- late blight, early blight and virosis；Stem green, branch ability is strong, and leaf yield is high；Common blade profile, petiole green, vein
Yellow green, blade kermesinus, its dry astaxanthin average content reach 2.02mg/g.Calyx green, radial corolla yellow is extremely
Chinese red, flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit is orange red, fruit shoulder aubergine, oblong, fruit
Shape index 1.07~1.25, fruit surface smooth, gloss is bright, and pulp is thick, and ventricle 4, hardness is big, mean fruit weight 120g, maximum single
Fruit weight is up to more than 200g.
Compared with prior art, excellent benefit of the invention is, the tomato variety cultivated by breeding method of the present invention
' cloud shrimp No. 1 ' reaches 2mg/g as objective trait using dry matter weight of leaf astaxanthin average content, and Fruit Characteristics are secondary character, are taken into account
Resistance and stability etc., whether it is that kermesinus is used as the mark property for differentiating Hybrid and ' cloud is anti-No. 1 ' using plant color.Knot
The measure for closing Phenotypic Observation and blade content astaxanthin is assessed, and ' cloud shrimp No. 1 ' is combined with the main merit of Parent, disease-resistant
Property strong and high-yield astaxanthin.It is mainly characterized by, plant one dimensional infinite wall, more than plant height 200cm, and growth potential is extremely strong；Anti- late blight
Disease, early blight and virosis；Stem green is to kermesinus, and branch ability is strong, and leaf yield is high；Common blade profile, petiole yellow green is to dark
Red, vein yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reach 2.02mg/g.Calyx is yellowish green
Color is to kermesinus, and radial corolla yellow to Chinese red, flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit orange
Red, fruit shoulder aubergine, oblong, fruit shape index 1.07~1.25, fruit surface smooth, gloss is bright, and pulp is thick, ventricle 4, firmly
Degree is big, and mean fruit weight 120g, maximum single fruit weight is up to more than 200g.
Embodiment：
The essentiality content of the present invention is further illustrated with the concrete instance of the present invention below, but is not limited with this
The present invention.
Embodiment 1：
The hybridizing method of tomato variety ' cloud shrimp No. 1 '：
With composite-crossing method, so that ' mango -1 ' is maternal, ' CLN2037E ' is ' cloud resists No. 1 ' that paternal hybrid comes to be
Female parent, obtained with producing astaxanthin engineering tomato ' red No. 1 of shrimp ' for paternal hybrid.
Maternal ' cloud is anti-No. 1 ' tomato breeding lines are Yunnan Province " 15 " Program for Tackling Key Problems《High-quality tomato hybrid new breed seed selection and
Supporting development technique research》One of achievement, last 3 years by tomato project team of gardens College of Horticulture of Yunnan Prov Agriculture University within 2007
Seed selection success.Male parent ' red No. 1 of shrimp ' is production astaxanthin engineering tomato, cultivated and forms in 2009, is (the Huang JC such as yellow pretty tide
Deng 2013) screen and be separated to from green alga by gene engineering method and can be catalyzed beta carotene and maize in plant cell
The assimilation enzyme and '-hydroxylase gene of plain synthesizing astaxanthin are transferred in wild-type tomatoes plant (cv.UC82B), obtained transgenosis kind
Eggplant is (on May 14th, 2014 in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, preserving number
For：CGMCC No 9223, Classification And Nomenclature：' red No. 1 of shrimp ' transgene tomato).2010 to 2012 respectively in Hong Kong University
Bioscience institute and Kunming Inst. of Botany, Chinese Academy of Sciences have carried out the plantation of 4 generations by potted plant mode, and character is stable.
Start to sow within 2013, ' red No. 1 of shrimp ', full-bloom stage pollination the previous day in May, will ' cloud is anti-No. 1 ' flower than ' cloud is anti-No. 1 ' early sowing 20 days
Hat elongation is exposed, and color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, is opened in next day
Colored bud emasculation is clean, pays attention to isolation.When morning dew is done, collection male parent ' red No. 1 of shrimp ' petal deploys 180 ° or so and flower
The red flower of colour cast, choose stamen in indoor tweezers, spread out and dry in the air on thin and flexible blank sheet of paper, indicate the collection date, be placed in
Naturally dry in the shade in clean place.Anther dehiscence is treated, when pollen sheds, it is standby to collect pollen.Second day, after maternal field dew is dry,
Fine day is pollinated in morning 8-9 points.Specifically, dipping a small amount of pollen point with the rubber tip of common pencil invests maternal emasculation
It is placed on the column cap of flower or by pollen on blank sheet of paper, contacts the column cap of emasculation, directly dips a small amount of pollen.With 6 × 8cm tea bags
Bag bagging, paper clip are fixed, listing mark, and Parent combination number and pollination date are indicated on plate.Bag taking after pollinating 10 days,
Situation of bearing fruit is observed simultaneously, in time training cleaning, remove the bud do not pollinated and newborn inflorescence, training are pruned, extract more
Remaining axillary bud and the branch of excessive growth, during which pay attention to liquid manure coordinated management and the preventing and treating of pest and disease damage.It can adopt within about 50~60 days after pollination
Receive ripening fruits.Unmarked fallen fruits and malformed fruit are not harvested, only harvest the normal fruit that grows.Complete red ripe fruit
After real harvesting, can after-ripening take kind again within 1~2 day.When taking kind, laterally fruit is splitted with knife, then squeezed into seed and glue flesh
Ferment in anhydrous nonmetallic vessel, fermentation cleans seed after 1~2 day, and the place for being placed in divulging information, drying is filtered dry moisture and immediately
Dry, until seed moisture content is stored when being down to 7%~8%, sowing in March in next year.Treat tomato variety ' cloud shrimp No. 1 ' after planting
Plant grows up, and the lower 4-5 layer blades in its top of timely collecting, freeze-drying obtains dry powder.Sample 50mg accurately is weighed, adds acetone extraction
4 times, until extract is colourless, collects extract and be settled to 5ml, loading determines pigment after suction filtration, sets 3 repetitions, using super
High performance liquid chromatography (UPLC) method determines the content of its astaxanthin.By 4 times of 2014 and 2015 plantations, Phenotypic Observation and
The measurement result of blade content astaxanthin shows that ' cloud shrimp No. 1 ' character is excellent neat, and blade content astaxanthin is high, and disease resistance
By force.
The character for the hybrid strain that hybridizing method of the present invention is related to is as follows：
' cloud anti-No. 1 ' female parent is：' mango -1 ', selected by tomato project team of gardens College of Horticulture of Yunnan Prov Agriculture University through 3 years
The self-mating system educated, there is the characters such as high anti-late blight, early blight and virosis, high yield, fruit orange colour, a single fruit weight 200g left sides
It is right.
' cloud anti-No. 1 ' male parent is：' CLN2037E ', introduced by Asia Vegetable Research centre of development (Taiwan), through Yunnan agriculture
The self-mating system that the generation of tomato project team of study column woods College of Horticulture of sparetime university 3 obtains after purification, there is high anti-late blight, early blight and virus
The characters such as disease, high yield, fruit are large red, and single fruit weight is 60g or so.
' cloud is anti-No. 1 '：Seed selection in 3 years was lasted by tomato project team of gardens College of Horticulture of Yunnan Prov Agriculture University in 2007, it is planted
Strain is one dimensional infinite wall, and more than plant height 200cm, growth is strong, belongs to middle-late ripening variety；Fruit large red, uniform coloring are long oval
Shape, fruit surface smooth, gloss is bright, and pulp is thick, and ventricle 4, hardness is big, and storage tolerance is good, shelf life length, mean fruit weight 120g,
Maximum single fruit weight, in Kunming Areas spring greenhouse cultivation, can be harvested as 180 days or so up to more than 200g from being seeded into.
Produce astaxanthin engineering tomato ' red No. 1 of shrimp '：Its plant, flower, leaf, fruit are main morphologically with Lycopersicon esculentum indifference
If the difference in color.Specifically, plant one dimensional infinite wall, plant height 110cm or so.The erect type of stem half, stem thick 2.6~
4.7cm, 4~6 branches, yellow green is to light brown.Common blade profile, petiole green to kermesinus, blade kermesinus.Calyx is dark red
Color, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit is subsphaeroidal, into
Chinese red, mean fruit weight 100g or so after ripe, the average value of its fruit content astaxanthin is 1.63mg/g.
The new varieties ' cloud shrimp No. 1 ' formed, which are cultivated, through composite-crossing is combined with the main merit of 3 parents, disease resistance
By force, high-yield astaxanthin.It is mainly characterized by, and ' cloud shrimp No. 1 ' is combined with the main merit of Parent, and disease resistance is strong and high yield
Astaxanthin.It is mainly characterized by, plant one dimensional infinite wall, more than plant height 200cm, and growth potential is extremely strong；Anti- late blight, early blight and
Virosis；Stem green is to kermesinus, and branch ability is strong, and leaf yield is high；Common blade profile, petiole yellow green to kermesinus, vein are yellow
Green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.02mg/g.Calyx yellow green to kermesinus,
For radial corolla yellow to Chinese red, flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit is orange red, and fruit shoulder is purple
Red, oblong, fruit shape index 1.07~1.25, fruit surface smooth, gloss is bright, and pulp is thick, and ventricle 4, hardness is big, average single
Fruit weight 120g, maximum single fruit weight is up to more than 200g.
Claims (9)
1. the breeding method of tomato variety ' cloud shrimp No. 1 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting, kind
Subprocessing, content astaxanthin determination step, using by ' mango -1 ' is ' cloud anti-1 that maternal, CLN2037E obtains as paternal hybrid
Number ' for female parent, production astaxanthin engineering tomato ' red No. 1 of shrimp ' it is that male parent is hybridized, with acetone extraction blade astaxanthin, and use
UPLC methods detect its content, and described pollen collection step is that for male parent than maternal early sowing 20 days, the full-bloom stage morning treated that dew is done
Afterwards, gather male parent ' red No. 1 of shrimp ' petal and deploy 170-190 ° and the partially red flower of pattern, choose stamen, spread out on paper, indicate product
Kind name and collection date, dry in the shade naturally, treat Anther dehiscence, when pollen sheds, it is standby to collect pollen；Described maternal emasculation is,
Pollination the previous day selection ' cloud anti-No. 1 ' corolla elongation is exposed, color from green to yellow, petal do not open or crack, flower pesticide in yellow green,
But shed pollen, the bud bloomed in next day of not ftractureing carries out emasculation；Described artificial pollination is after maternal field dew is done, fine
Pollinated when its morning 8-9 point, 20~25 DEG C of temperature；Described fruit harvesting is 50~60 days harvesting ripening fruits after pollination；
After described seed treatment is complete red ripe fruit harvesting, after-ripening takes kind in 1~2 day again；Described content astaxanthin determines
With acetone extraction blade astaxanthin, UPLC methods detect its content.
2. the breeding method of tomato variety ' cloud shrimp No. 1 ' as claimed in claim 1, it is characterised in that：Described pollen collection
The pollen collected in step is temporarily stored in 4 DEG C of refrigerators, and long-term storage silica gel is made drier and preserved in -80 DEG C of refrigerators.
3. the breeding method of tomato variety ' cloud shrimp No. 1 ' as claimed in claim 1, it is characterised in that：Described maternal emasculation
Step is that bud base portion is surely held with left hand, and the right hand clamps flower pesticide with tweezers and all extracts its stamen, or is together shelled together with petal
Fall, emasculation is clean, isolates after emasculation, while rejects the flower for being not suitable for pollination and selfing fruit.
4. the breeding method of tomato variety ' cloud shrimp No. 1 ' as claimed in claim 1, it is characterised in that：Described artificial pollination
Step temperature dips a small amount of pollen point at 20~25 DEG C, with the rubber tip of common pencil and invested on the column cap of maternal emasculation flower,
Or directly pollen is placed on blank sheet of paper, the column cap of emasculation is directly contacted, dips a small amount of pollen, with 6 × 8cm tea bag bag baggings,
Paper clip is fixed, listing mark, and Parent combination number and pollination date are indicated on plate, and bag taking after pollinating 10 days, observation is sat
Fruit situation, training cleaning in time, removes the bud do not pollinated and the inflorescence of new life, training are pruned, extract unnecessary axillary bud and
The branch of excessive growth, carry out liquid manure coordinated management and the preventing and treating of pest and disease damage.
5. the breeding method of tomato variety ' cloud shrimp No. 1 ' as claimed in claim 1, it is characterised in that：Described fruit harvesting
In only harvesting grow normal fruit, do not harvest unmarked fallen fruits and malformed fruit.
6. the breeding method of tomato variety ' cloud shrimp No. 1 ' as claimed in claim 1, it is characterised in that：Described seed treatment
Fruit is laterally splitted in step, seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, to fermentate surface
For white, stirred with wooden stick, seed sinks, and seed is pinched with hand, and pectin separates with seed, without seed is cleaned when sticking sliding sense, removes
Pulp, debris and flat seed, load in double gauze bag and squeeze the water out, be spread out on the overhead canvas in bottom or blotting paper and dry in the shade, turn over
It is dynamic the moisture of the surface of the seed is evaporated rapidly several times, until when seed moisture content is down to 7%~8%, paper bag packing, normal temperature
Storage, sowing in March in next year.
7. the breeding method of tomato variety ' cloud shrimp No. 1 ' as claimed in claim 1, it is characterised in that：Described astaxanthin contains
It is that plant to be planted is grown up to measure determination step, the lower 4-5 floor blades in collection tomato variety ' cloud shrimp No. 1 ' top, freeze-dried acquisition blade
Dry powder, sample 50mg is weighed, add acetone extraction 4 times, until extract is colourless, collected extract and be settled to 5ml, loading after suction filtration
Pigment is determined, 3 repetitions are set, the content of its astaxanthin is determined using ultra performance liquid chromatography UPLC methods.
8. the breeding method of tomato variety ' cloud shrimp No. 1 ', it is characterised in that it comprises the following steps：(1) pollen collection, male parent ratio
Maternal early sowing 20 days, the full-bloom stage morning, collection male parent ' red No. 1 of shrimp ' petal deploys 170-190 ° and pattern is partially red after dew is dry
Flower, choose stamen in indoor tweezers, spread out on thin and flexible blank sheet of paper, indicate kind name and collection the date, be placed in
Clean place is dried in the shade naturally, treats Anther dehiscence, and when pollen sheds, collection pollen is standby, and pollen is stored temporarily in 4 DEG C of refrigerators,
Long-term storage is stored in -80 DEG C of refrigerators with silica gel as drier；(2) maternal emasculation, pollination the previous day selection ' cloud is anti-No. 1 ' flower
Hat elongation is exposed, and color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, is opened in next day
Colored bud carries out emasculation, and during emasculation, left hand surely holds bud base portion, and the right hand clamps flower pesticide with tweezers and all extracts its stamen,
Or together peeled off together with petal, isolate after emasculation, while reject the flower for being not suitable for pollination and selfing fruit；(3) artificial pollination, it is female
Start to pollinate after Honda dew is dry, fine day is carried out in morning 8-9 points, and suitable temperature of pollinating is 20~25 DEG C, with common pencil
Rubber tip dip a small amount of pollen, point is invested on the column cap of maternal emasculation flower or pollen is placed on blank sheet of paper, directly contact
The column cap of emasculation, a small amount of pollen is dipped, with 6 × 8cm tea bag bag baggings, paper clip is fixed, listing mark, and father and mother are indicated on plate
This combination number and pollination date, bag taking after pollinating 10 days, while situation of bearing fruit is observed, training cleaning in time, remove and do not pollinate
Bud and new life inflorescence, training prunes, and extracts unnecessary axillary bud and the branch of excessive growth, carries out liquid manure coordinated management and disease pest
Harmful preventing and treating；(4) fruit harvesting, 50~60 days harvesting ripening fruits, do not harvest unmarked fallen fruits and malformed fruit after pollination,
Only harvest the normal fruit that grows；(5) after seed treatment, completely red ripe fruit harvesting, after-ripening takes kind for 1~2 day again, uses
Knife laterally splits fruit, and seed and glue flesh are squeezed into anhydrous nonmetallic vessel and fermented 1~2 day, until fermentate surface
White, stirred with wooden stick, seed sinks, and seed is pinched with hand, and pectin separates with seed, without seed is cleaned when sticking sliding sense, uses clear water
Rinsing, pulp, debris and flat seed are removed, load in double gauze bag and squeeze the water out, be spread out in the overhead canvas in bottom or blotting paper
On, stir, the moisture of the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%~8%, paper bag packing, normal temperature
Storage, sowing in March in next year；(6) content astaxanthin determines, and New Tomato Variety ' cloud shrimp No. 1 ' after planting grow up by plant to be planted, harvesting top
Lower 4-5 layer blades, freeze-dried acquisition blade dry powder, weigh sample 50mg, add acetone extraction 4 times, until extract is colourless,
Collect extract and be settled to 5ml, loading determines pigment after suction filtration, sets 3 repetitions, is surveyed using ultra performance liquid chromatography UPLC methods
The content of its fixed astaxanthin.
9. the breeding method of the tomato variety ' cloud shrimp No. 1 ' as described in claim 1 or 8, it is characterised in that it is described with by
' mango -1 ' is maternal, CLN2037E is that ' cloud is anti-No. 1 ' that paternal hybrid obtains is maternal, produces astaxanthin engineering tomato ' shrimp red 1
Number ' it is that the biological characteristics that male parent carries out ' cloud shrimp No. 1 ' that hybridization cultivation obtains is：' cloud shrimp No. 1 ' is combined with the master of Parent
Want merit, disease resistance is strong and high-yield astaxanthin, it is characterized in that, plant one dimensional infinite wall, more than plant height 200cm, growth potential
It is extremely strong；Anti- late blight, early blight and virosis；Stem green is to kermesinus, and branch ability is strong, and leaf yield is high；Common blade profile, petiole
Yellow green to kermesinus, vein yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.02mg/
G, calyx yellow green to kermesinus, radial corolla yellow to Chinese red, flower pesticide is orange to red, column cap and to harbor young fruit reddish brown
Color, fruit is orange red, and fruit shoulder aubergine, oblong, fruit shape index 1.07~1.25, fruit surface smooth, gloss is bright, and pulp is thick,
Ventricle 4, hardness is big, and mean fruit weight 120g, maximum single fruit weight reaches more than 200g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610003043.4A CN106106121B (en) | 2016-01-04 | 2016-01-04 | A kind of breeding method of leaf production astaxanthin tomato variety |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610003043.4A CN106106121B (en) | 2016-01-04 | 2016-01-04 | A kind of breeding method of leaf production astaxanthin tomato variety |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106106121A CN106106121A (en) | 2016-11-16 |
| CN106106121B true CN106106121B (en) | 2017-11-21 |
Family
ID=57269744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610003043.4A Expired - Fee Related CN106106121B (en) | 2016-01-04 | 2016-01-04 | A kind of breeding method of leaf production astaxanthin tomato variety |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106106121B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577254B (en) * | 2016-11-30 | 2019-03-22 | 福建省农业科学院茶叶研究所 | A kind of Tool Box and its pollinating method improving tea tree artificial pollination efficiency |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6861580B2 (en) * | 2002-01-03 | 2005-03-01 | Harris Moran Seed Company | Inbred tomato line 294 |
| CN104145678B (en) * | 2014-08-15 | 2016-02-24 | 中国科学院昆明植物研究所 | A kind of cultivation method promoting transgene tomato growth and astaxanthin accumulation |
| CN104206139B (en) * | 2014-08-15 | 2016-02-24 | 中国科学院昆明植物研究所 | A kind of method improving content astaxanthin in astaxanthin-containing transgene tomato output and transgene tomato |
-
2016
- 2016-01-04 CN CN201610003043.4A patent/CN106106121B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN106106121A (en) | 2016-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103975849B (en) | A kind of precocity, the breeding of disease-resistant, the pale reddish brown Broccoli of loose type and breeding method | |
| CN103703974A (en) | Method for cultivating sweet potatoes | |
| Tammaro | Saffron (Crocus sativus L.) in Italy | |
| CN107432243B (en) | A kind of selection of cold-resistant, resistance to bolting cabbage | |
| CN113080056B (en) | Cultivation method of ginseng and Ji fruit | |
| Sharma et al. | Pigeonpea | |
| CN106106122B (en) | Leaf produces the breeding method of astaxanthin tomato variety | |
| CN106106121B (en) | A kind of breeding method of leaf production astaxanthin tomato variety | |
| CN103988775A (en) | Method for breeding and cultivating early ripening disease-resistant stem broccoli | |
| CN107455255B (en) | A kind of selection of broccoli | |
| CN111642388B (en) | Creating method of excellent germplasm of red flower ginkgo strawberry | |
| CN109329043A (en) | A kind of selection of small seed type pumpkin rootstock kind | |
| CN101720664A (en) | Cultivation method of oranges | |
| CN105918109B (en) | A kind of fast dish selection of purple | |
| CN101861828B (en) | Pineapple hybridization method | |
| CN106613895B (en) | Leaf produces the breeding method of astaxanthin tomato | |
| Opeña et al. | Hybrid seed production in tomato | |
| CN102246693B (en) | Method for creating new peanut specie in space breeding | |
| Singh et al. | Studies on Parkia timoriana (DC.) Merr: an endemic and economically important tree species of north east India | |
| CN105638449B (en) | A kind of high yield, long fruit pod, single pod seediness grain oil radish selection | |
| LU505436B1 (en) | Breeding method for blight-resistant bitter gourd pistillate line | |
| Qin et al. | Responses of hypocotyl elongation to light and sowing depth in peanut seedlings | |
| CN112544366B (en) | Cultivation method of passion fruit seedlings | |
| NL2032712B1 (en) | Artificial hybridization breeding method of mango | |
| Uretsky | Development and evaluation of interspecific Cucurbita maxima x Cucurbita moschata hybrids for processing squash |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171121 Termination date: 20200104 |