CN107455255B - A kind of selection of broccoli - Google Patents
A kind of selection of broccoli Download PDFInfo
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- CN107455255B CN107455255B CN201710806430.6A CN201710806430A CN107455255B CN 107455255 B CN107455255 B CN 107455255B CN 201710806430 A CN201710806430 A CN 201710806430A CN 107455255 B CN107455255 B CN 107455255B
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Abstract
The invention discloses a kind of selection of broccoli, former broccoli self incompatible line (male parent) and broccoli (fertile, maternal) are first hybridized by the emasculation florescence, and the florescence is returned between four generations, then florescence plant through honeybee free pollination again for rear emasculation.On the one hand, purificated and rejuvenated original parent, on the other hand, by each generation, repeatedly the florescence pollinates repeatedly, weaken original parental autocopulation incompatibility, self-compatibility enhancing, is gradually changed into self-mating system, for utilizing cytoplasm male sterility line breeding, improve breeding efficiency, the shortcomings that self incompatible line breeding needs artificial flower bud phase cabage-type rape is overcome, while the phenomenon that degenerating due to many years selfing parental trait of also purificating and rejuvenating, as plant growing way is strong, it is few to lose flower bud rate, pollen amount is more, setting percentage height etc., has found new method for broccoli purification and rejuvenation.
Description
Technical field
The present invention relates to a kind of selections of broccoli, belong to broccoli breeding technical field.
Background technique
Broccoli (Brassica deracea L.var italic P) also known as broccoli, broccoli belong to Cruciferae rue
Using green bouquet as a mutation of product in a kind of sedge category brassica specie, it is rich for the tender scape of green children and bud, nutrition to eat part
Richness contains protein, sugar, fat, vitamin and carrotene, has antitumaous effect containing giucosinolate, deep by the joyous of consumer
It meets, has become international market top grade green vegetables.Broccoli has stomach invigorating aid digestion and is rich in anthocyanidin and cancer-resisting substance,
In cancer-resisting substance it is high, be a kind of delicious high-quality with health care perfect combination, high-grade functional form green vegetables.Currently,
The positive large area of Vegetable Base plants broccoli, to supply domestic and international market.
From late nineteen nineties, China starts to have introduced some external kinds, nearly ten years, broccoli cultivated area broccoli
Rapid growth, a lot of country's broccoli seeds still need to external import, and seed price is expensive.First-filial generation at present in production is advantageous
A large amount of manpower and time is spent to carry out parent with self incompatible line preparing hybrid kind, but using the self incompatible line production of hybrid seeds
It breeds (passing through artificial bud pollination), cost is very high, and popularizing area is restricted by production of hybrid seeds amount.More disadvantageously parent is continuous for a long time certainly
Transaction generates viability and degenerates, and lose some excellent characters can, the hybrid rate of cenospecies is extremely difficult to 100%.It is also advantageous
With the method for dominant genic male sterility preparing hybrid kind, but dominant genic male sterility is cumbersome, complicated, is not easy to push away
Extensively.It is more using cytoplasmic male sterility preparing hybrid kind, what it is due to harvesting is nutrition organs, is not seed, does not need
Restorer, it is only necessary to which male parent system, male parent system can be self-mating system and be also possible to self incompatible line, can using the method production of hybrid seeds
To overcome a series of defects for utilizing the production of hybrid seeds such as self incompatible line, the hybrid rate 100% of cenospecies.Therefore new varieties, green wood
The breeding of material, to foreign monopolization is broken, the kind for promoting independent intellectual property right is had a very important significance.
In Middle And Lower Reaches of Changjiang River is suitble to broccoli ecological breeding, has accumulated to breeding broccoli new varieties new material abundant
Germ plasm resource.From original self incompatible line preparing hybrid kind, cytoplasm male sterility line preparing hybrid kind is arrived, needs to change breeding
Mode, from a large amount of screening self incompatible line parent materials to the screening of self-mating system parent material.The excellent selfing originally accumulated
Not affine system's parent material is on the verge of being replaced or upgrading, such as long-term continuous selfing are also easy to produce viability degeneration, make some excellent
Character lose, if plant growing way is weak, lose that flower bud rate is high, and pollen amount tails off, the hybrid rate of cenospecies is extremely difficult to 100%, special
It is not that self incompatible line needs artificial flower bud phase cabage-type rape, the disadvantages of heavy workload, parent propagation is difficult.Not with cytoplasmic male
The extensive utilization for being is educated, how the merit of the excellent broccoli self incompatible line parent material of past accumulation is purified
Rejuvenation preservation is gone down, and be changed into self-compatibility by self-incompatibility, for broccoli material breeding provide it is some newly
Method is even more important.
Summary of the invention
Goal of the invention: in order to solve the above-mentioned technical problems, the present invention provides a kind of selections of broccoli.
Technical solution: to achieve the goals above, the invention discloses a kind of selections of broccoli, including following step
It is rapid:
(1) First Year, broccoli self incompatible line parent material are sowed at the beginning of 10 months, are colonized crop field at the beginning of 11 months.From October
Just after planting to second year florescence in March, weak seedling is pulled out in field observation screening, different strain, and it is strong to select and remain growth potential, anti-sclerotiniose, black
Maize ear rot is strong, and growth is neat, and bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good, and stamen prosperity etc. is most
There is the typical plant of this parent material for parental plant 3-5 plants of purification and rejuvenation.This 3-5 plants at the beginning of parent material second year 5 months from plant
Clip axillary bud carries out tissue cultures in strain, and numerous by subculture, expansion, annual tissue cultures 15-65 plants spare, as purification and rejuvenation parent
This typical case plant (male parent), tissue culture propagation are studied referring to article broccoli male sterile line group culturation rapid propagating technology
(Zhang Zhen is superfine, Jiangsu's agriculture journal, 2011,27 (3): 680-681), similarly hereinafter, seedling are tamed and dociled at the beginning of the laboratory cultures, annual 10 months
Change, is colonized crop field at the beginning of 11 months.
(2) second year March, the excellent broccoli of precocity of 3-5 plants of above-mentioned purification and rejuvenation parent typical case plant and Field Screening
For F1 kind (fertile) in peak period of blooming, every plant is stayed 3-5 branch, is stayed second day bud that will be opened about 10-15, has been opened
The flower put, small bud are all extractd, and manually shell female parent of the whole strain bagging isolation as first-filial generation after flower bud emasculation, third day
It is used as male parent by above-mentioned 3-5 plants of purification and rejuvenation parent typical case plant, pollen mixture painting, which is touched, hybridizes one after above-mentioned emasculation
It pollinates and hybridizes on the column cap of godmother's sheet, obtain F1 first-filial generation seed.
(3) second year October, above-mentioned acquisition F1 first-filial generation seed, sowing at the beginning of 10 months, field planting mid-November, laboratory are every
Year 15-65 plants of optimum periods domestications of purification and rejuvenation parental plant of tissue cultures are colonized, and are after planting bloomed from early October to March next year
Phase investigates Biology character compared with parent typical case plant (male parent) with purificating and rejuvenating, and weak seedling is pulled out in field observation screening,
Different strain, selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong, and growth is neat, and bouquet is semicircle, and flower bud development is normal, nectary hair
It reaches, stigma development is good, and 4 plants of plant of the typical case such as stamen prosperity are used as first backcross generation plant (female parent).March next year is in Sheng of blooming
Phase, every plant of above-mentioned (female parent) stay 5 branches, stay second day bud that will be opened about 10-15, the flower opened, small flower
Flower bud is all extractd, while the bud that second day will be opened carries out whole strain bagging isolation after artificial stripping flower bud emasculation, wherein 2 plants
(female parent) third day morning 9-10 point touches the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) after emasculation
Column cap pollination, 3-4 point is repeated to apply and be touched 1 time in the afternoon, and morning next day 9-10 point repeats once, this 2 plants (female parent) are returned
Generation seed BC1 makees backcrossing of lower generation female parent, obtains first backcross generation seed and is denoted as the backcrossing female parent that B-grade in the first class C1 makees lower generation；It is 2 plants another (female
This), the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) is touched the column after emasculation by third day morning 9-10 point
Head pollination, acquisition first backcross generation seed are denoted as second BC1 and compare, and investigate seed-setting rate, florescence self-compatable index respectively.
(4) third year, above-mentioned 2 plants of (female parent) B-grade in the first class C1, above-mentioned another 2 plants of (female parent) second BC1, sowing, mid-November at the beginning of 10 months
Separately field planting, 15-65 plants of optimum period domestication field planting of purification and rejuvenation parental plant of laboratory tissue culture, from early October after planting extremely
Weak seedling is pulled out in florescence March next year, field observation screening, different strain, selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong respectively,
Growth is neat, and bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good, the plant such as stamen prosperity and with purification
Most similar each 2 plants of rejuvenation parent typical case plant (male parent) are used as second backcross generation plant (female parent).March next year in peak period of blooming,
2 plants of B-grade in the first class C1,2 plants of second BC1 respectively stay 5 branches, stay second day bud that will be opened about 10-15, the flower opened,
Small bud is all extractd, while the bud that second day will be opened carries out whole strain bagging isolation after artificial stripping flower bud emasculation.First
2 plants of third day morning 9-10 points of BC1 touch the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) after emasculation
Column cap pollination, 3-4 point in afternoon repeat apply touches 1 time, morning next day 9-10 point repeats once, will purification and rejuvenation parent typical case plant
The pollination of the column cap after emasculation is touched in the pollen mixture painting of strain (male parent), this 2 plants (female parent) acquisition second backcross generation seeds are denoted as first
It is maternal that BC2 makees the backcrossing of lower generation.2 plants of second BC1 compare, third day morning 9-10 point, respectively plant purification and rejuvenation parent typical case
The pollination of the column cap after emasculation is touched in the pollen mixture painting of strain (male parent), and acquisition second backcross generation seed is denoted as second BC2 and makees lower generation time
Maternal control is handed over, investigates seed-setting rate, florescence self-compatable index respectively.
(5) the 4th years, 2 plants of above-mentioned B-grade in the first class C2,2 plants of seeds of second BC2, sowing, was separately colonized mid-November at the beginning of 10 months,
15-65 plants of optimum period domestication field planting of purification and rejuvenation parent typical case plant of laboratory tissue culture, from early October after planting to next year 3
Weak seedling is pulled out in month florescence, field observation screening, different strain, selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong respectively, is grown whole
Together, bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good, stamen prosperity etc. and with purification and rejuvenation parent's allusion quotation
Each 2 plants of the most similar plant of type plant (male parent) are used as third backcross generation plant (female parent).March next year in peak period of blooming, B-grade in the first class C2's
2 plants, 2 plants of second BC2 are respectively stayed 5 branches, stay second day bud that will be opened about 10-15, the flower opened, small flower
Flower bud is all extractd, while the bud that second day will be opened carries out whole strain bagging isolation after artificial stripping flower bud emasculation.The 2 of B-grade in the first class C2
The pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) is touched the column cap after emasculation by strain third day morning 9-10 point
Pollination, 3-4 point in afternoon are repeated to apply and be touched 1 time, and morning next day 9-10 point repeats once, by the parent typical case plant (father that purificates and rejuvenates
Originally the pollination of the column cap after emasculation is touched in pollen mixture painting), this 2 plants (female parent) acquisition third backcross generation's seeds are denoted as B-grade in the first class C3 work
Lower generation backcrossing is maternal.2 plants of second BC2 compare, third day morning 9-10 point, and will purificate and rejuvenate parent typical case plant (father respectively
Originally the pollination of the column cap after emasculation is touched in pollen mixture painting), and acquisition third backcross generation's seed is denoted as second BC3 and makees backcrossing of lower generation female parent
Seed-setting rate, florescence self-compatable index are investigated in control respectively.
(6) the 5th years, 2 plants of above-mentioned B-grade in the first class C3,2 plants of seeds of second BC3, sowing, was separately colonized mid-November at the beginning of 10 months,
15-65 plants of optimum period domestication field planting of purification and rejuvenation parent typical case plant of laboratory tissue culture, from early October after planting to next year 3
Weak seedling is pulled out in month florescence, field observation screening, different strain, selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong respectively, is grown whole
Together, bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good, stamen prosperity etc. and with purification and rejuvenation parent's allusion quotation
Each 2 plants of the most similar plant of type plant (male parent) as four generation plant (female parent) of backcrossing.March next year is in peak period of blooming, above-mentioned first
2 plants of BC3,2 plants every plant of second BC3 stay 5 branches, stay second day bud that will be opened about 10-15, opened
Flower, the bud that small bud is all extractd, while second day will be opened carry out whole strain bagging isolation after artificial stripping flower bud emasculation,
Wherein 2 plants of B-grade in the first class C3, third day morning 9-10 point touch the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent)
Column cap pollination after emasculation, 3-4 point in afternoon are repeated to apply and be touched 1 time, and morning next day 9-10 point repeats once, by the parent that purificates and rejuvenates
The pollination of the column cap after emasculation is touched in the pollen mixture painting of typical plant (male parent), this 2 plants (female parent) acquisitions are returned four generation seeds
It is denoted as B-grade in the first class C4, investigates seed-setting rate, florescence self-compatable index.2 plants of second BC3 compare, third day morning 9-10 point,
The pollination of the column cap after emasculation is touched into the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) respectively, obtains backcrossing four
Second BC4 is denoted as seed and makees the maternal control of backcrossing of lower generation, investigates seed-setting rate, florescence self-compatable index respectively.
(7) the 6th years, 2 plants of above-mentioned B-grade in the first class C4,2 plants of seeds of second BC4, sowing, was separately colonized mid-November at the beginning of 10 months,
Separately field planting compares for 15-65 plants of optimum period domestications of purification and rejuvenation parent typical case plant of laboratory tissue culture, broadcasts from early October
To the plant that florescence March next year, above-mentioned B-grade in the first class C4 are colonized after kind, field observation screening pulls out weak seedling, different strain selects and remain growth potential
By force, anti-sclerotiniose, black rot are strong, and growth is neat, and bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good,
Stamen prosperity etc. and individually it is isolated with purification and rejuvenation parent typical case plant (male parent) most similar plant as new parental plant.
March next year in florescence by honeybee free pollination, the seed harvested on new parental plant, parent's kind after as purificating and rejuvenating
Son as original silkworm egg, and investigates seed-setting rate, florescence self-compatable index.The plant of above-mentioned second BC4 field planting, selects and remain 2 plants, and 1
Every plant is stayed 5 branches in peak period of blooming March next year for strain, is stayed second day bud that will be opened about 10-15, has been opened
Flower, the bud that small bud is all extractd, while second day will be opened carry out whole strain bagging isolation after artificial stripping flower bud emasculation,
The pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) is touched the column cap after emasculation and awarded by third day morning 9-10 point
Powder obtains five generation seeds of backcrossing and is denoted as second BC5 (florescence), and investigates seed-setting rate, florescence self-compatable index.Another 1 plant time
March in year, every plant was stayed 5 branches, and the bud that morning 9-10 point will open second and third day carries out artificial stripping flower bud in peak period of blooming
The pollination of the column cap after having shelled flower bud emasculation is touched in the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) after emasculation (to have opened
The flower put, small bud are all extractd), whole strain bagging isolation obtains five generation seeds of backcrossing and is denoted as second BC5 (flower bud phase), and investigates
Seed-setting rate, flower bud phase self-compatable index.
Entire breeding period administration is as follows:
(1) prepare before nursery, build greenhouse, select topography dry, greenhouse is built in place that is flat, having drained, takes shelter from rain, is high
Warm Shad density fly net；Matrix selects vegetables special seedling substrate (the Denmark's Pin Shi matrix sold in the market, matrix organic
Total amount >=25, nitrogen, phosphorus, potassium total content >=2, total porosity 50-70% are rich in microelement, and nutrient is comprehensive, also rich in other
Active promoting root growth substance.PH6.0-7.0, cushion performance are good)；
(2) it sows, Nutrition Soil is filled up in the hole tray and is compacted, treated the seed of every bunch planting one, then use nutrition
Soil covering, surface strikes off, is put into preprepared sink, is watered with water, spreads newspaper above, with sharp moisturizing；
(3) seedling management, Qi Miaohou remove newspaper in time, and fine day is sufficiently light-exposed by seedling, when noon temperature is higher than 30 DEG C
Lid sunshade net.No matter fine day keeps matrix to moisten degree with the cloudy day, (yellowish with stromal surface, matrix hand pinches a little water outlet i.e.
Can) depending on；Moisture is controlled after Qi Miao；Ventilation is kept in canopy, keeps matrix wet, and appropriate ventilation reduces humidity in canopy.
(4) Chicken dung 2500kg/667 ㎡ is applied in site preparation and fertilization field site preparation and fertilization, crop field, N:P:K=15:15:15's
Ternary compound fertilizer 50kg/667 ㎡ is ploughed in conjunction with rake and is applied down, is uniformly mixed with soil, shallow ridges flat furrow is made into when playing furrow, even ditch is made into
The flat furrow of 1.3m, furrow ditch depth 15cm, the wide 30cm of furrow ditch, and build greenhouse.
(5) it is colonized, is colonized crop field when seedling has 5~6 leaves, the seeding row spacing ㎝ of 40 ㎝ × 40 pours root water.
(6) field management is combined to loosen the soil and shallowly be earthed up, and promotes plant strain growth.Second year beginning of spring temperature is at 10 DEG C or more
Overlay film rain shelter cultivation, surrounding do not set apron.Fine day noon cuts bouquet with scraper, guarantees that bouquet is loose, and 300 times of stones are touched in incision painting
Sulphur mixture, and siphonal lobe, old leaf are removed in time, prevent and treat pest and disease damage.
(7) after blooming period management bolting, a bamboo bar is inserted, with increasing for bolting, successive 2~3 bondage lodging-preventions, each
Cell is strictly isolated with 60 mesh yarn accounts.Bolting latter stage successively carries out pinching, defloration to coming into bloom during this period, regulation florescence,
Xie Huahou.Secondary flower is extractd, in time to promote full seed.
(8) prevention and control of plant diseases, pest control.It is prevented and treated cabbage caterpillar 1 time with Dipel (Bt)；With 10% imidacloprid wettable powder aphid
Worm 2 times.With 75% Bravo wettable powder, 600 times of liquid, with 75% sclerotium spirit wettable powder, 500 times of liquid prevention and treatment downy mildew,
Sclerotiniose each 1 time.
(9) it is harvested when seed collection kind pod 2/3 turns yellow, plants grain flavescence brown, is conducted batch-wise daily, keeps seed as complete as possible
It is complete mature, and sunning in time, threshing, bask seeds, the storage preservation when seed moisture content is up to 7% or less.
Technical effect: compared with the existing technology, present invention has the advantage that
Former broccoli self incompatible line (male parent) and broccoli (fertile, maternal), are first hybridized by the emasculation florescence, after go
The male florescence again, which was returned between four generations, then florescence strain, passes through honeybee free pollination (breeding is referring to attached drawing 1).On the one hand, it purificates and rejuvenates
Parent originally, on the other hand, by each generation, repeatedly the florescence pollinates repeatedly, weakens original parental autocopulation incompatibility,
Self-compatibility enhancing, is gradually changed into self-mating system, for improving breeding efficiency using cytoplasm male sterility line breeding, gram
The shortcomings that self incompatible line breeding needs artificial flower bud phase cabage-type rape has been taken, while also having been purificated and rejuvenated since many years are selfed parent's property
The phenomenon that shape is degenerated loses that flower bud rate is few, and pollen amount is more if plant growing way is strong, setting percentage height etc. (referring to table 1- table 4), multiple to purify
It is strong to have found new method.
Detailed description of the invention
Fig. 1 is 0918 (new) parent of broccoli purification and rejuvenation map of the present invention.
Specific embodiment
With reference to the accompanying drawing and specific example, the present invention is furture elucidated, it should be understood that these examples are merely to illustrate this hair
Bright rather than limit the scope of the invention, after the present invention has been read, those skilled in the art are to of the invention various etc.
The modification of valence form falls within the application range as defined in the appended claims.
Embodiment 1:
1,2010, broccoli self incompatible line parent material 0918 totally 1, flower bud phase self-compatable index was 9.3, flower
Phase self-compatable index 0.26.Sowing on October 15th, 2010, field planting on November 20 crop field.Field management is referring to entire breeding time
It manages (1)-(9).From sowing to florescence in March, 2011, weak seedling is pulled out in field observation screening, different strain, and it is strong to select and remain growth potential,
Anti- sclerotiniose, black rot are strong, and growth is neat, and bouquet is semicircle, and top bouquet is small and loose, bud is thin, stem length, and lateral branching is sprouted
Power is strong, and flower bud development is normal, and nectary is flourishing, and stigma development is good, and the typical of the most parent material 0918 such as stamen prosperity is planted
Strain stays 5 plants to purificate and rejuvenate parent typical case plant (male parent).The morning for parent typical case plant (male parent) and proper seeding of purificating and rejuvenating
Green F1 kind (fertile) screens 2 plants of green F1 plant (female parent) in stalwartness in plant flowering ripe excellent broccoli.2011
March, every plant of above-mentioned 2 plants (female parents) stayed 5 branches, and every plant is stayed the second day bud that will be opened about 10-15 in peak period of blooming
Flower that is a, having opened, it is latter that the bud that small bud is all extractd, while second day will be opened carries out artificial stripping flower bud emasculation
And be isolated with 60 mesh yarn accounts, third day morning 9-10 point touches the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent)
The column cap after emasculation, which has been pollinated, hybridizes, and obtains first cross seed F1 about 270, florescence self-compatable index is 9.1.
2, from 15-20 progress laboratory of clip axillary bud in purification and rejuvenation parent typical case plant (male parent) at the beginning of 5 months 2011
Tissue cultures, numerous by subculture, expansion, annual tissue cultures 15-65 plants are used as purification and rejuvenation parent typical case plant (male parent), for after
It is used for breeding, seedling is colonized crop field in domestication at the beginning of laboratory cultures, 10 months, optimum period at the beginning of annual 11 months.
3, in October, 2011, above-mentioned 2 plants (female parents) obtain F1 first cross seed, 150 are sowed at the beginning of 10 months, mid-November
130 plants of field planting is tamed, field planting mid-November at the beginning of 20 plants of the purification and rejuvenation parent typical case plant of laboratory tissue culture 10 months, from
After planting to florescence in March, 2012 at the beginning of 10 months, with investigation Biology compared with parent typical case plant (male parent) of purificating and rejuvenating
Character, field observation screening, pulls out weak seedling, different strain, selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong, and growth is neat, bouquet
Semicircle, top bouquet is small and loose, bud is thin, stem length, and lateral branching germination is strong, and flower bud development is normal, and nectary is flourishing, column cap
It physically well develops, 4 plants of typical plant of stamen prosperity etc. are used as first backcross generation plant (female parent).In March, 2012 in peak period of blooming, on
It states every plant of (female parent) and stays 5 branches, stay second day bud that will be opened about 10-15, the flower opened, small bud is complete
Portion is extractd, while the bud that second day will be opened be isolated together with 60 mesh yarn accounts after manually shelling flower bud emasculation.Wherein 2 plants
(female parent), third day morning 9-10 point touch the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) after emasculation
Column cap pollination, 3-4 point in afternoon are repeated to apply and be touched 1 time, and morning next day 9-10 point repeats once, this 2 plants (female parent) acquisition backcrossings one
It is denoted as the backcrossing female parent that B-grade in the first class C1 makees lower generation for seed, obtains first backcross generation seed B-grade in the first class C1 about 120, florescence self-compatable index
8.6.Another 2 plants (female parents), third day morning 9-10 point, respectively by the pollen mixture painting of parent typical case plant (male parent) of purificating and rejuvenating
The pollination of the column cap after emasculation is touched, acquisition first backcross generation seed is denoted as second BC1 and makees the maternal control of backcrossing of lower generation, obtains and be returned one
For seed second BC1 about 80, florescence self-compatable index 6.1.
4,2012, above-mentioned 2 plants of (female parent) B-grade in the first class C1, above-mentioned another 2 plants of (female parent) second BC1 seed, sowing 110 on October 12
Grain, 75, November 15 was colonized 105 plants, 70 plants respectively, was separately colonized, and the purification and rejuvenation parent of laboratory tissue culture is typical
It is tamed at the beginning of 30 plants of plant 10 months, the field planting of optimum period November 18, the plant of B-grade in the first class C1, second BC1 field planting, from early October after planting extremely
Weak seedling is pulled out in florescence in March, 2013, field observation screening, different strain, and it is strong to select and remain growth potential respectively, anti-sclerotiniose, black rot
By force, growth is neat, and bouquet is semicircle, and top bouquet is small and loose, bud is thin, stem length, and lateral branching germination is strong, and flower bud development is just
Often, nectary is flourishing, and stigma development is good, stamen prosperity etc. and with purification and rejuvenation parent typical case plant (male parent) most similar each 2
Strain is used as second backcross generation plant (female parent).In March, 2013 in peak period of blooming, 2 plants of B-grade in the first class C1,2 plants of second BC1 respectively stay 5 branches
Item stays second day bud that will be opened about 10-15, and the flower opened, small bud was all extractd, while by second day
The bud that will be opened is isolated with 60 mesh yarn accounts together after carrying out artificial stripping flower bud emasculation, wherein the 2 of B-grade in the first class C1 plant (female parent), third
The pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) is touched the pollination of the column cap after emasculation by its morning 9-10 point, under
Noon 3-4 point is repeated to apply and be touched 1 time, and morning next day 9-10 point repeats once, this 2 plants (female parent) acquisition second backcross generation seeds are denoted as first
BC2 makees backcrossing of lower generation female parent, obtains second backcross generation seed B-grade in the first class C2 about 91, florescence self-compatable index 7.6.2 plants of second BC1
It compares, third day morning 9-10 point, the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) is touched gone respectively
Column cap pollination after hero, acquisition second backcross generation seed are denoted as second BC2 and make the maternal control of backcrossing of lower generation, obtain second backcross generation seed second
BC2 about 58, florescence self-compatable index 4.8.
5,2013,2 plants of above-mentioned B-grade in the first class C2,2 plants of seeds of second BC2, October 14 Bo Zhong not be 80, and 50, November 18
It is colonized 70 plants respectively, 45 plants, is separately colonized, is tamed and dociled at the beginning of 25 plants of the purification and rejuvenation parent typical case plant of laboratory tissue culture 10 months
Change, field planting on November 18, the plant of B-grade in the first class C2, second BC2 field planting, from early October after planting to florescence in March, 2014, field is seen
Screening to be examined, pulls out weak seedling, different strain selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong respectively, and growth is neat, and bouquet is semicircle,
Top bouquet is small and loose, bud is thin, stem length, and lateral branching germination is strong, and flower bud development is normal, and nectary is flourishing, and stigma development is good
It is good, stamen prosperity etc. and with most similar each 2 plants of purification and rejuvenation parent typical case plant (male parent) it is (female as third backcross generation plant
This).In March, 2014 in peak period of blooming, 2 plants of B-grade in the first class C2,2 plants of second BC2 respectively stay 5 branches, stay second day flower that will be opened
Flower bud about 10-15, the flower opened, small bud is all extractd, while the bud that second day will open manually being shelled
It is isolated together with 60 mesh yarn accounts after flower bud emasculation, wherein the 2 of B-grade in the first class C2 plant (female parent), third day morning 9-10 point will purificate and rejuvenate close
The pollination of the column cap after emasculation is touched in the pollen mixture painting of this typical case plant (male parent), and 3-4 point in afternoon, which repeats to apply, touches 1 time, next day
Morning 9-10 point repeats once, this 2 plants (female parent) acquisition third backcross generation's seeds are denoted as B-grade in the first class C3 and make backcrossing of lower generation female parent, obtains
Third backcross generation's seed B-grade in the first class C3 about 72, florescence self-compatable index 5.1.2 plants of second BC2 compare, third day morning 9-10
The pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) is touched the pollination of the column cap after emasculation respectively, is returned by point
It hands over three generations's seed to be denoted as second BC3 and makees the maternal control of backcrossing of lower generation, obtain third backcross generation's seed second BC3 about 31, florescence selfing parent
With index 3.6.
6,2014,2 plants of above-mentioned B-grade in the first class C3,2 plants of seeds of second BC3 sowed 70 on October 10 respectively, and 30, November
It is colonized within 11st 60 plants, 25 plants respectively, is separately colonized, at the beginning of 35 plants of the purification and rejuvenation parent typical case plant of laboratory tissue culture 10 months
Domestication, field planting on November 10.The plant of B-grade in the first class C3, second BC3 field planting, from early October after planting to florescence in March, 2015, field
Observation screening, pulls out weak seedling, different strain, selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong respectively, and growth is neat, bouquet semicircle
Shape, top bouquet is small and loose, bud is thin, stem length, and lateral branching germination is strong, and flower bud development is normal, and nectary is flourishing, stigma development
Well, stamen prosperity etc. and with most similar each 2 plants of purification and rejuvenation parent typical case plant (male parent) it is (female as four generation plant of backcrossing
This).In March, 2015 in peak period of blooming, 2 plants of B-grade in the first class C3,2 plants of second BC3 respectively stay 5 branches, stay second day flower that will be opened
Flower bud about 10-15, the flower opened, small bud is all extractd, while the bud that second day will open manually being shelled
It is isolated together with 60 mesh yarn accounts after flower bud emasculation.Wherein 2 plants (female parents) of B-grade in the first class C3, third day morning 9-10 point will purificate and rejuvenate close
The pollination of the column cap after emasculation is touched in the pollen mixture painting of this typical case plant (male parent), and 3-4 point in afternoon, which repeats to apply, touches 1 time, next day
Morning 9-10 point repeats once, this 2 plants (female parent) acquisitions are returned four generation seeds and are denoted as B-grade in the first class C4, obtains four generation seed first of backcrossing
BC4 about 42, florescence self-compatable index 4.1.2 plants of second BC3 compare, third day morning 9-10 point, respectively that purification is multiple
The pollination of the column cap after emasculation is touched in the pollen mixture painting of strong parent typical case plant (male parent), is obtained four generation seeds of backcrossing and is denoted as second
BC4 makees the maternal control of backcrossing of lower generation, obtains four generation seed second BC4 about 11 of backcrossing, florescence self-compatable index 2.1.
7,2015,2 plants of above-mentioned B-grade in the first class C4,2 plants of seeds of second BC4 sowed 40,10, November October 13 respectively
It is colonized within 16th 35 plants, 8 plants respectively, separately cultivates, at the beginning of 20 plants of the purification and rejuvenation parent typical case plant of laboratory tissue culture 10 months
Domestication, field planting on November 16.From early October after planting to florescence in March, 2016, the plant of above-mentioned B-grade in the first class C4 field planting, field is seen
Screening is examined, pulls out weak seedling, different strain selects and remain that growth potential is strong, and anti-sclerotiniose, black rot are strong, and growth is neat, and bouquet is semicircle, top
Bouquet is small and loose, bud is thin, stem length, and lateral branching germination is strong, and flower bud development is normal, and nectary is flourishing, and stigma development is good, male
Stamen prosperity etc. and individually it is isolated with the pure most similar plant of rejuvenation parent typical case plant (male parent) as 20 plants new of parental plant.
In March, 2016 in florescence by honeybee free pollination, about 3 grams of seed harvested on above-mentioned 20 plants of new parental plants, for purification
New parent seed after rejuvenation, as original silkworm egg, florescence self-compatable index is 6.1.The plant of above-mentioned second BC4 field planting, choosing
2 plants are stayed, wherein 1 plant of in March, 2016, in peak period of blooming, every plant is stayed 5 branches, stays the second day bud that will be opened about 10-15
Flower that is a, having opened, small bud are all extractd, while whole after the artificial stripping flower bud emasculation of bud progress that second day will be opened
Strain bagging isolation, third day morning 9-10 point touch the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) in emasculation
Column cap pollination afterwards obtains five generation BC5 seeds of backcrossing and is denoted as second BC5 (florescence) 2, florescence self-compatable index 0.4；Another 1 plant
In March, 2016, every plant was stayed 5 branches, and the bud that morning 9-10 point will open second and third day carries out artificial in peak period of blooming
The pollination of the column cap after having shelled flower bud emasculation is touched into the pollen mixture painting of purification and rejuvenation parent typical case plant (male parent) after stripping flower bud emasculation
(flower opened, small bud are all extractd), in whole strain bagging isolation, acquisition five generations of backcrossing, are denoted as second BC5 (flower bud phase), and seed is about
45, flower bud phase self-compatable index 8.9.
Entire breeding period administration is as follows:
(1) prepare before nursery, build greenhouse, select topography dry, greenhouse is built in place that is flat, having drained, takes shelter from rain, is high
Warm Shad density fly net；Matrix selects vegetables special seedling substrate (the Denmark's Pin Shi matrix sold in the market, matrix organic
Total amount >=25, nitrogen, phosphorus, potassium total content >=2, total porosity 50-70% are rich in microelement, and nutrient is comprehensive, also rich in other
Active promoting root growth substance.PH6.0-7.0, cushion performance are good)；
(2) it sows, Nutrition Soil is filled up in the hole tray and is compacted, treated the seed of every bunch planting one, then use nutrition
Soil covering, surface strikes off, is put into preprepared sink, is watered with water, spreads newspaper above, with sharp moisturizing；
(3) seedling management, Qi Miaohou remove newspaper in time, and fine day is sufficiently light-exposed by seedling, when noon temperature is higher than 30 DEG C
Lid sunshade net.No matter fine day keeps matrix to moisten degree with the cloudy day, (yellowish with stromal surface, matrix hand pinches a little water outlet i.e.
Can) depending on；Moisture is controlled after Qi Miao；Ventilation is kept in canopy, keeps matrix wet, and appropriate ventilation reduces humidity in canopy.
(4) Chicken dung 2500kg/667 ㎡ is applied in site preparation and fertilization field site preparation and fertilization, crop field, N:P:K=15:15:15's
Ternary compound fertilizer 50kg/667 ㎡ is ploughed in conjunction with rake and is applied down, is uniformly mixed with soil, shallow ridges flat furrow is made into when playing furrow, even ditch is made into
The flat furrow of 1.3m, furrow ditch depth 15cm, the wide 30cm of furrow ditch, and build greenhouse.
(5) it is colonized, is colonized crop field when seedling has 5~6 leaves, the seeding row spacing ㎝ of 40 ㎝ × 40 pours root water.
(6) field management is combined to loosen the soil and shallowly be earthed up, and promotes plant strain growth.Second year beginning of spring temperature is at 10 DEG C or more
Overlay film rain shelter cultivation, surrounding do not set apron.Fine day noon cuts bouquet with scraper, guarantees that bouquet is loose, and 300 times of stones are touched in incision painting
Sulphur mixture, and siphonal lobe, old leaf are removed in time, prevent and treat pest and disease damage.
(7) after blooming period management bolting, a bamboo bar is inserted, with increasing for bolting, successive 2~3 bondage lodging-preventions, each
Cell is strictly isolated with 60 mesh yarn accounts.Bolting latter stage successively carries out pinching, defloration to coming into bloom during this period, regulation florescence,
Xie Huahou.Secondary flower is extractd, in time to promote full seed.
(8) prevention and control of plant diseases, pest control.It is prevented and treated cabbage caterpillar 1 time with Dipel (Bt)；With 10% imidacloprid wettable powder aphid
Worm 2 times.With 75% Bravo wettable powder, 600 times of liquid, with 75% sclerotium spirit wettable powder, 500 times of liquid prevention and treatment downy mildew,
Sclerotiniose each 1 time.
(9) it is harvested when seed collection kind pod 2/3 turns yellow, plants grain flavescence brown, is conducted batch-wise daily, keeps seed as complete as possible
It is complete mature, and sunning in time, threshing, bask seeds, the storage preservation when seed moisture content is up to 7% or less.
Advantage: former broccoli self incompatible line (male parent) and broccoli (fertile, maternal), it is first miscellaneous by the emasculation florescence
It hands over, the florescence is returned between four generations, then florescence plant through honeybee free pollination again for rear emasculation (breeding is referring to attached drawing 1).On the one hand, it purifies
The original parent of rejuvenation, on the other hand, by each generation, repeatedly the florescence pollinates repeatedly, makes original parental autocopulation incompatibility
Weaken, self-compatibility enhancing is gradually changed into self-mating system, for utilizing cytoplasm male sterility line breeding, improves breeding effect
Rate overcomes the shortcomings that self incompatible line breeding needs artificial flower bud phase cabage-type rape, while also purificating and rejuvenating since many years are selfed
The phenomenon that parental trait is degenerated loses that flower bud rate is few, and pollen amount is more if plant growing way is strong, and setting percentage height etc. (referring to table 1- table 4) is
Purification and rejuvenation have found new method.
The new backcross progeny morphological variation of table 1 0918 performance
Table 2 0918 and the florescence in each generation and flower bud phase self-compatable index
(new) florets feature of table 3 0918 and 0918 and Flowering Characteristics
The main plant character of table 4 0918 and 0918 (new) parent
Claims (8)
1. a kind of selection of broccoli, which comprises the following steps:
(1) First Year, broccoli self incompatible line parent material are sowed at the beginning of 10 months, are colonized crop field at the beginning of 11 months, broadcast from early October
To second year florescence in March after kind, weak seedling is pulled out in field observation screening, different strain, and it is strong to select and remain growth potential, anti-sclerotiniose, black rot
By force, growth is neat, and bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good, stamen prosperity most this parent
The typical plant of this material is parental plant 3-5 plants of purification and rejuvenation, this 3-5 plants is cut from plant at the beginning of parent material second year 5 months
Axillary bud is taken to carry out tissue cultures, numerous by subculture, expansion, annual tissue cultures 15-65 plants spare, and parent is typical as purification and rejuvenation
Plant, similarly hereinafter, seedling are colonized crop field at the beginning of 11 months in domestication at the beginning of laboratory cultures, annual 10 months；
(2) second year March, the fertile excellent broccoli of precocity of 3-5 plants of above-mentioned purification and rejuvenation parent typical case plant and Field Screening
For F1 kind in peak period of blooming, every plant is stayed 3-5 branch, stays second day bud that will be opened about 10-15 a, the flower opened,
Small bud is all extractd, and manually shells female parent of the whole strain bagging isolation as first-filial generation after flower bud emasculation, and third day will be above-mentioned
3-5 plants of purification and rejuvenation parent typical case plant be used as male parent, it is maternal that pollen mixture is applied to first-filial generation after above-mentioned emasculation
Column cap on pollinate hybridization, obtain F1 first-filial generation seed；
(3) second year October, above-mentioned acquisition F1 first-filial generation seed, sowing at the beginning of 10 months, field planting mid-November, annual group of laboratory
Purification and rejuvenation parental plant 15-65 plants of optimum periods domestication field planting of culture is knitted, from early October after planting to florescence March next year, with
Purification and rejuvenation parent typical case plant compares investigation Biology character, and field observation screening pulls out weak seedling, different strain selects and remain life
Growing way is strong, and anti-sclerotiniose, black rot are strong, and growth is neat, and bouquet is semicircle, and flower bud development is normal, and nectary is flourishing, and stigma development is good
Good, flourishing 4 plants of the typical plant of stamen is used as first backcross generation plant；March next year, above-mentioned every plant was stayed 5 branches in peak period of blooming, and was stayed
About 10-15, second day bud that will be opened, the flower opened, small bud is all extractd, while second day will be opened
The bud put carries out whole strain bagging isolation after artificial stripping flower bud emasculation, the parent wherein 2 plants of third day morning 9-10 points will purificate and rejuvenate
The pollen mixture of typical plant is applied to the pollination of the column cap after emasculation, and 3-4 point repeats to smear 1 time in the afternoon, morning next day 9-
10 points repeat once, this 2 plants acquisition first backcross generation seed BC1 make backcrossing of lower generation female parent, obtain first backcross generation seed and are denoted as first
The backcrossing that BC1 makees lower generation is maternal；Another 2 plants, third day morning 9-10 point applies the pollen mixture for parent typical case plant of purificating and rejuvenating
Column cap pollination after being put on emasculation, acquisition first backcross generation seed are denoted as second BC1 and compare, and investigate seed-setting rate, flower respectively
Phase self-compatable index；
(4) third year, above-mentioned 2 plants of B-grade in the first class C1, above-mentioned another 2 plants of second BC1, sowing, is separately colonized, laboratory mid-November at the beginning of 10 months
Purification and rejuvenation parental plant 15-65 plants of optimum periods domestication field planting of tissue cultures, from early October after planting to florescence March next year,
It is selected by step (3) standard and purificates and rejuvenates most similar each 2 plants of parent typical case plant as second backcross generation plant, March next year
In peak period of blooming, 2 plants of B-grade in the first class C1,2 plants of second BC1 press step (3) identical processing method, obtain second backcross generation seed respectively
It is denoted as B-grade in the first class C2 and second BC2, makees backcrossing of lower generation female parent and the maternal control of backcrossing of lower generation respectively, respectively investigation seed-setting rate, the florescence
Self-compatable index；
(5) the 4th years, 2 plants of above-mentioned B-grade in the first class C2,2 plants of seeds of second BC2, sowing, was separately colonized mid-November at the beginning of 10 months, tests
15-65 plants of optimum period domestication field planting of purification and rejuvenation parent typical case plant of room tissue cultures, are after planting opened to March next year from early October
Florescence is selected by step (3) standard and purificates and rejuvenates each 2 plants of the most similar plant of parent typical case plant as third backcross generation's plant
Strain, March next year in peak period of blooming, 2 plants of B-grade in the first class C2,2 plants of second BC2 press step (3) identical processing method, returned respectively
It hands over three generations's seed to be denoted as B-grade in the first class C3 and second BC3, makees backcrossing of lower generation female parent and the maternal control of backcrossing of lower generation respectively, investigate seed respectively
Setting percentage, florescence self-compatable index；
(6) the 5th years, 2 plants of above-mentioned B-grade in the first class C3,2 plants of seeds of second BC3, sowing, was separately colonized mid-November at the beginning of 10 months, tests
15-65 plants of optimum period domestication field planting of purification and rejuvenation parent typical case plant of room tissue cultures, are after planting opened to March next year from early October
Florescence is selected by step (3) standard and purificates and rejuvenates each 2 plants of the most similar plant of parent typical case plant as four Dai Zhi of backcrossing
Strain, March next year in peak period of blooming, 2 plants of above-mentioned B-grade in the first class C3,2 plants of second BC3 press step (3) identical processing method, obtain respectively
Four generation seeds must be returned and be denoted as B-grade in the first class C4 and second BC4, investigate seed-setting rate, florescence self-compatable index respectively；
(7) the 6th years, 2 plants of above-mentioned B-grade in the first class C4,2 plants of seeds of second BC4, sowing, was separately colonized mid-November at the beginning of 10 months, tests
Separately field planting compares for 15-65 plants of optimum period domestications of purification and rejuvenation parent typical case plant of room tissue cultures, from early October after planting
To florescence March next year, the plant of above-mentioned B-grade in the first class C4 field planting is selected by step (3) standard and purificates and rejuvenates parent typical case plant most
Similar plant is individually isolated as new parental plant；March next year is in florescence by honeybee free pollination, new parental plant
The seed of upper harvest, the parental seed after as purificating and rejuvenating, as original silkworm egg, and investigates seed-setting rate, florescence selfing parent
And index；The plant of above-mentioned second BC4 field planting, selects and remain 2 plants, 1 plant of March next year, every plant was stayed 5 branches in peak period of blooming, and stayed second
About 10-15, its bud that will be opened, the flower opened, small bud is all extractd, while second day will be opened
Bud carries out whole strain bagging isolation after artificial stripping flower bud emasculation, and third day morning 9-10 point is mixed by parent typical case plant of purificating and rejuvenating
It closes pollen and is applied to the pollination of the column cap after emasculation, the backcrossing five generations seed for obtaining the florescence is denoted as second BC5, and investigates seed-setting
Rate, florescence self-compatable index；Another 1 plant of March next year, every plant was stayed 5 branches in peak period of blooming, morning 9-10 point by second and third
The pollen mixture for parent typical case plant of purificating and rejuvenating is applied to after the artificial stripping flower bud emasculation of its bud that will be opened progress and has been shelled
Column cap pollination after flower bud emasculation, the flower opened, small bud are all extractd, and whole strain bagging isolation obtains the backcrossing five of flower bud phase
It is denoted as second BC5 for seed, and investigates seed-setting rate, flower bud phase self-compatable index.
2. the selection of broccoli according to claim 1, which is characterized in that need to build greenhouse before breeding, select
Topography is dry, and place that is flat, having drained builds greenhouse, takes shelter from rain, high temperature Shad density fly net；Matrix selects vegetables nursery special
Use matrix.
3. the selection of broccoli according to claim 1, which is characterized in that the type of seeding are as follows: in hole tray
It fills up Nutrition Soil and is compacted, treated the seed of every bunch planting one, then covered with Nutrition Soil, surface strikes off, and is put into advance
Ready sink is watered with water, spreads newspaper above, with sharp moisturizing；
Seedling management method are as follows: Qi Miaohou removes newspaper in time, and fine day is sufficiently light-exposed by seedling, when noon temperature is higher than 30 DEG C
Lid sunshade net；Depending on no matter fine day and cloudy day keep matrix to moisten degree；Moisture is controlled after Qi Miao；Ventilation is kept in canopy,
Keep matrix wet, appropriate ventilation reduces humidity in canopy；
Site preparation and fertilization method are as follows: Chicken dung 2500kg/667m is applied in field site preparation and fertilization, crop field2, the three of N:P:K=15:15:15
Component composite fertilizer 50kg/667 m2, plough and apply down in conjunction with rake, be uniformly mixed with soil, shallow ridges flat furrow are made into when playing furrow, even ditch is made into
The flat furrow of 1.3m, furrow ditch depth 15cm, the wide 30cm of furrow ditch, and build greenhouse.
4. the selection of broccoli according to claim 1, which is characterized in that the method for the field planting are as follows: have to seedling
Crop field is colonized when there are 5~6 leaves, the cm of seeding row spacing 40cm × 40 pours root water.
5. the selection of broccoli according to claim 1, which is characterized in that in the selection, field management
Method are as follows: shallowly earthed up in conjunction with loosening the soil, promote plant strain growth, second year beginning of spring temperature overlay film at 10 DEG C or more is taken shelter from rain cultivation
Training, surrounding do not set apron, and fine day noon cuts bouquet with scraper, guarantee that bouquet is loose, and incision smears 300 times of lime sulfurs, and
Removal siphonal lobe, old leaf in time prevents and treats pest and disease damage.
6. the selection of broccoli according to claim 1, which is characterized in that in the selection, blooming period management
Method are as follows: after bolting, a bamboo bar is inserted, with increasing for bolting, successive 2~3 bondage lodging-preventions, 60 mesh yarns of each cell
Account is strictly isolated, and bolting latter stage successively carries out pinching, defloration to coming into bloom during this period, and regulation florescence, Xie Huahou is plucked in time
Except secondary flower, to promote full seed.
7. the selection of broccoli according to claim 1, which is characterized in that in the selection, pest and disease damage is anti-
Control method are as follows: use Bacillus thuringiensis Prevention cabbage caterpillar 1 time；With 10% imidacloprid wettable powder aphid 2 times, with 75,%00 bacterium
600 times of liquid of clear wettable powder prevent and treat downy mildew, sclerotiniose each 1 time with 75% sclerotium spirit wettable powder, 500 times of liquid.
8. the selection of broccoli according to claim 1, which is characterized in that in the selection, seed collection
Method are as follows: kind pod 2/3 turns yellow, kind grain flavescence brown when harvest, be conducted batch-wise daily, make seed full maturity as far as possible, and and
When sunning, threshing, bask seeds, when seed moisture content is up to 7% or less, storage is saved.
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| CN101263781A (en) * | 2007-03-15 | 2008-09-17 | 浙江神良种业有限公司 | Broccoli male sterility line transfer and hybrid preparation method |
| CN102835306A (en) * | 2012-09-26 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
| CN105075854A (en) * | 2015-09-21 | 2015-11-25 | 镇江瑞繁农艺有限公司 | Breeding method for early-maturing and disease-resistant broccoli |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101263781A (en) * | 2007-03-15 | 2008-09-17 | 浙江神良种业有限公司 | Broccoli male sterility line transfer and hybrid preparation method |
| CN102835306A (en) * | 2012-09-26 | 2012-12-26 | 江苏丘陵地区镇江农业科学研究所 | Breeding method for early-maturing hybrid broccoli in additive selection |
| CN105075854A (en) * | 2015-09-21 | 2015-11-25 | 镇江瑞繁农艺有限公司 | Breeding method for early-maturing and disease-resistant broccoli |
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